Search Results (7,680)

Hexavalent chromium (Cr(VI)) contamination in agricultural soils poses a significant risk to environmental and food safety owing to its high mobility and acute toxicity. To investigate possible mitigation strategies, a greenhouse pot experiment was conducted using sandy loam and silty loam soils spiked with Cr(VI) at 30 mg kg⁻¹ and amended with natural clinoptilolite and modified HDTMA-Br (hexadecyl-trimethyl-ammonium-bromide) zeolite, while celery (Apium graveolens) was cultivated to assess chromium bioavailability and plant accumulation. Hexavalent chromium concentrations declined in all treatments (up to 88.2% in sandy loam and 73.5% in silty loam), indicating progressive reduction to Cr(III), although amendment effectiveness varied by soil type. In addition, celery accumulated extremely high chromium concentrations, particularly in sandy loam soil, where root Cr(VI) reached 1776 mg kg⁻¹, indicating substantial safety concerns. Translocation factor values were below 1 across treatments, indicating limited relocation of Cr from roots to shoots. In the zeolite treatments, Cr(VI) concentrations in aboveground biomass decreased; however, plant uptake was not completely inhibited. Nonetheless, the high bioaccumulation factor (Cr in plant over available Cr in soil) of as high as 34 in the Cr(VI)-amended treatment indicated an uptake potential under Cr load. We conclude that modified zeolite was successful in mitigating Cr(VI) uptake in plants. Further investigation on the effectiveness of the materials in open-field conditions is required to establish a remediation framework for Cr species Full article

Background/Objectives: This regional case study evaluated the affordability and serving accuracy of a publicly available one-week DASH meal plan for single-woman households using Supplemental Nutrition Assistance Program (SNAP) benefits in central Kentucky. Methods: For each food item in the one-week plan, total grocery costs and per-serving costs were calculated using January 2025 prices from two national grocery chains commonly patronized in an urban area in central Kentucky. Calculated costs were compared to the average weekly food cost for women aged 20–50 years in a single household based on the Thrifty Food Plan (TFP). Servings for food groups and categories were calculated using MyPlate and American Heart Association guidelines to compare with those reported in the one-week plan. Results: The total grocery cost was $262.17, including staple foods expected to last more than a week. The adjusted per-serving cost of $82.90 was 21.19% higher than the average weekly food cost based on the January 2025 TFP. All food groups and categories except dairy showed differences of at least one serving between our calculations and the one-week plan. Conclusions: Findings from this case study on grocery costs suggest that the one-week plan may pose affordability challenges in this regional context and continued evaluation of whether SNAP benefit allotments based on TFP adequately reflect regional food cost variations may be warranted. Discrepancies in total servings highlight the need to improve the accuracy of publicly available DASH resources and to review these materials for consistency and accuracy. Full article

The global rise in obesity and metabolic disorders has intensified interest in dietary bioactives capable of improving glycemic control and metabolic health. Inositols, particularly D-pinitol, have emerged as insulin-sensitizing cyclitols with potential metabolic relevance. Carob (Ceratonia siliqua L.), one of the richest natural sources of D-pinitol, represents a promising nutritional matrix for metabolic regulation. This narrative review critically evaluates current evidence on the role of D-pinitol in glucose homeostasis and energy balance, integrating data from chemical characterization studies, mechanistic research, preclinical models, and human clinical trials assessing purified D-pinitol and D-pinitol–rich preparations, particularly from carob-derived sources. Available evidence suggests that D-pinitol may enhance insulin signaling efficiency, primarily through PI3K/Akt-dependent pathways, modulate hepatic metabolic flexibility, and influence endocrine balance without acting as a classical hypoglycemic agent. Preclinical models consistently report improvements in insulin sensitivity, lipid handling, oxidative stress parameters, and tissue-specific metabolic adaptations. In contrast, clinical trials in healthy, prediabetic, and type 2 diabetic individuals show more heterogeneous outcomes, including attenuation of postprandial glycemia, reductions in circulating insulin and HOMA-IR, and modest improvements in lipid and inflammatory markers. Overall, carob-derived D-pinitol appears to act as a potential insulin-sensitizing metabolic modulator with context-dependent effects influenced by metabolic phenotype and food matrix composition. However, available data remains limited and heterogeneous, with most data derived from preclinical studies and relatively small clinical trials. These findings should therefore be interpreted with caution. Larger, longer-term randomized controlled trials using standardized preparations are required to establish clinical relevance and translational applicability. Notably, the contribution of other bioactive components within the carob matrix cannot be excluded. Full article

South Africa’s food system reflects a growing imbalance between nutritionally rich indigenous foods and the increasing dominance of commercially processed alternatives. Despite well documented health, cultural, and environmental benefits, indigenous leafy vegetables and edible insects remain marginal within formal markets and everyday diets. This systematic review synthesised 141 empirical and theoretical studies to examine how household attitudes, consumption behaviours, and market structures interact to shape the role of indigenous foods in South Africa. The review identifies a consistent pattern in which positive perceptions of indigenous foods do not translate into regular consumption. Rural households continue to utilise these foods as part of seasonal and livelihood strategies, while uptake in urban areas remains uneven and context specific. Emerging interest among certain consumer segments highlights potential for product diversification and market development, particularly where indigenous foods are adapted to align with modern preferences. However, this potential is constrained by weak value chain integration, limited standardisation, and the absence of reliable consumption data. These structural limitations restrict both market participation and consumer access, reinforcing the peripheral position of indigenous foods within the broader food system. The findings suggest improving availability, strengthening markets, and enhancing positioning critical for inclusion of indigenous foods in diets. Full article

Genetically modified (GM) lactic acid bacteria (LAB) are gaining attention as tools for innovation in the food sector, health applications, and industrial processes. LAB have long been used safely due to their GRAS/QPS status, making them suitable for improving fermentation and synthesizing specific and beneficial metabolites. Advances in genomics and gene editing have significantly expanded the available tools, ranging from classical mutagenesis to site-specific recombination, homologous recombination in non-coding regions, CRISPR-based systems, and food-grade chromosomal integration. These approaches enable the insertion of desired genes and the development of engineered strains with tailored functionalities. GM-LAB are also being studied as live delivery systems for therapeutic molecules, including cytokines, hormones, antimicrobial peptides, and vaccine antigens. Engineered strains of Lactococcus lactis and Lactobacillus spp. have yielded promising outcomes in applications such as mucosal immunization, modulation of inflammatory and metabolic responses, and inhibition of pathogenic microorganisms, including multidrug-resistant bacteria. From an industrial perspective, several studies highlight their potential for cost-effective recombinant protein production and the synthesis of high-value metabolites through fermentation. However, within the European Union, their use is subject to stringent regulatory oversight, requiring comprehensive molecular and environmental risk assessments, careful evaluation of horizontal gene transfer, and a preference for markerless chromosomal integrations. Despite these constraints, GM-LAB offer significant potential to improve food quality, sustainability, and human health. Full article

Copper (Cu) is an essential micronutrient, but poorly controlled inputs may increase phytotoxicity risks and alter soil–plant nutrient dynamics. Therefore, Cu formulations that regulate rhizosphere Cu availability are of agronomic interest. This study compared non-encapsulated Cu nanoparticles (CuNPs) and alginate-encapsulated Cu nanoparticles (eCuNPs) in a 42-day pot experiment with Lactuca sativa L. grown in two agricultural soils with different properties, applying 0, 10, 25, 50, and 100 mg of Cu kg⁻¹. Soil properties, Rhizzo-extractable Cu as a proxy of available Cu, plant biomass, Cu accumulation, and nutrient concentrations were evaluated. Rhizzo-extractable Cu increased with dose under CuNPs, particularly in the soil with lower organic matter and ECEC, whereas eCuNPs maintained values closer to the control levels. In the soil with higher organic matter and ECEC, CuNPs were associated with reduced shoot and root biomass at higher doses, while eCuNPs showed a more variable response and, in some cases, higher biomass values. In contrast, biomass remained low across all treatments in the more limiting soil. Cu accumulated mainly in roots, and foliar Cu (FW) remained low and close to typical values reported for lettuce in the USDA FoodData Central database. Alginate encapsulation may reduce short-term Cu mobilization in the rhizosphere and could represent a promising strategy to improve the safety of CuNP applications, particularly in soils with higher organic matter and ECEC. Full article

Propolis has long been recognized for its biological properties, but its availability is increasingly threatened by apiary losses in agricultural areas. One response to this problem is the development of urban apiaries, located in urbanized environments and often established for educational and promotional purposes. In this study, edible pullulan films were prepared with the addition of 10, 20, and 30% propolis extract obtained from an urban apiary located in Toruń, Poland. The effect of these coatings applied to cherries on fruit spoilage, the growth inhibition of Aspergillus niger and Penicillium chrysogenum, and changes in pH, titratable acidity, soluble solids content, and fruit color were evaluated. The films showed both antimicrobial and antioxidant properties. The biological evaluation demonstrated dose-dependent antimicrobial activity, with inhibition zones ranging from 7.27 to 17.23 mm for fungi and from 7.09 to 16.22 mm for bacteria, with the strongest effects observed against L. monocytogenes, C. krusei, and P. chrysogenum. Antioxidant activity, determined using the DPPH radical scavenging assay, increased with propolis concentration and reached 29.44% for films containing 30% urban propolis extract. Moreover, pullulan coatings enriched with propolis reduced mold counts on cherries after 96 h of storage to 2.82 log CFU/g for P. chrysogenum and 2.72 log CFU/g for A. niger, compared with 7.02 and 7.17 log CFU/g, respectively, in uncoated fruit. The influence of urban propolis extract on the thickness and color of the obtained films was also demonstrated. It was found that pullulan coatings with urban propolis extract applied on cherries showed fungistatic properties against P. chrysogenum and A. niger. The developed films and coatings show potential for use in food technology to support the preservation of perishable raw materials; however, further studies are needed to confirm their effectiveness under broader storage conditions. Full article

Landmark epidemiological studies and clinical trials, such as the Seven Countries Study, the Lyon Diet Heart Study, the PREDIMED Study and the CORDIOPREV Study, have shown significant reductions in cardiovascular events in those following the Mediterranean diet (MD). The aim of the present work is to summarize the most robust available evidence and the major biological pathways underlying the protective effects of the MD, with particular emphasis on the role of PAF inhibitors. Mechanistically, MD functions through a complex synergy of antioxidant, anti-inflammatory, and antithrombotic effects that collectively improve lipid profiles, enhance endothelial function, optimize postprandial metabolism and cell membrane signaling, making it a functional model for human longevity. The PAF-Implicated Atherosclerosis Theory has emerged as a key unifying framework, proposing that Platelet-Activating Factor (PAF)—a highly potent lipid inflammatory mediator—plays a central role in the initiation and progression of atherosclerosis. Oxidized LDL promotes the production of PAF and PAF-like lipids, leading to endothelial dysfunction, vascular inflammation, and atherosclerotic plaque formation. Traditional Mediterranean foods are rich in natural PAF inhibitors, particularly the polar lipid fractions of extra virgin olive oil, as well as wine, fish, vegetables, onions, and garlic. Animal studies demonstrate that these compounds can reduce or even regress atherosclerotic lesions, independently of serum cholesterol levels. Human dietary interventions have further shown that MD-based meals and functional foods enriched with PAF inhibitors reduce PAF activity and improve thrombosis-related biomarkers. This mechanistic framework helps explain phenomena such as the “French Paradox” and the cardio-protective effects associated with fish consumption. Moreover, the extraction of PAF inhibitors from Mediterranean food by-products, such as olive pomace, offers promising ecological and economic advantages. Collectively, targeting PAF and increasing dietary intake of PAF inhibitors represent promising strategies for the prevention and management of atherosclerosis and other inflammatory diseases, supporting the view that PAF may function as a major, modifiable risk factor in these conditions. Full article

This study investigated the bactericidal effect and examined the associated cellular damage of low temperature plasma (LTP) combined with slightly acidic electrolyzed water (SAEW) against Listeria monocytogenes. Single-factor experiments were conducted to assess the bactericidal efficacy under individual treatment conditions, followed by the evaluation of three different combination sequences. An orthogonal experimental design was performed to optimize the key parameters, and the optimal treatment conditions were determined as LTP at 45 W with an electrode spacing of 1 mm for 2 min, combined with SAEW at an available chlorine concentration (ACC) of 30 mg/L. Under these conditions, confocal laser scanning microscopy (CLSM) with SYTO 9/PI staining confirmed that the combined treatment caused cell death, as indicated by loss of membrane integrity in treated cells. A resuscitation assay further ruled out the viable but non-culturable (VBNC) state, as no bacterial growth was detected after 48 h of enrichment. The leakage of intracellular proteins and nucleic acids was measured using the Coomassie Brilliant Blue method combined with a microplate reader, and changes in cellular morphology were observed by scanning electron microscopy (SEM). The results demonstrated that SAEW+LTP treatment exerted a distinct effect, significantly disrupting bacterial cell membrane integrity, inducing the leakage of intracellular contents, and causing obvious morphological damage to the bacterial cells. In conclusion, the combined treatment of LTP and SAEW significantly improved the bactericidal efficiency against L. monocytogenes, which may be due to the combined disruptive effects on membrane integrity and subsequent structural and functional damage to the cells. Future investigations are needed to unravel the precise mechanisms, establish the efficacy against a wider panel of strains, and explore the potential for practical application in food matrices. Full article

Efficient soil fertility monitoring is essential for sustainable agriculture, food security, and environmental management across Africa, yet conventional laboratory methods remain prohibitively costly and slow for continental-scale applications. Soil spectroscopy is considered as a rapid, non-destructive alternative with transformative potential. This review provides a systematic synthesis of spectroscopic applications across Africa, encompassing laboratory, field, airborne, and satellite-based platforms, while examining major data sources including the Africa Soil Information Service (AfSIS) and GEO-CRADLE spectral libraries. We critically evaluate the evolution of modeling approaches, revealing that Partial Least Squares Regression (PLSR) dominates, but a shift toward advanced frameworks like hybrid physically based models, ensemble learning and deep neural networks is essential. Critically, we identify a pronounced imbalance wherein laboratory spectroscopy prevails while imaging and satellite-based approaches remain comparatively underutilized, despite their unparalleled potential for scaling point measurements to continental extents. The review consolidates findings on key soil properties, demonstrating consistent successes for primary constituents with direct spectral responses (i.e., organic carbon), while revealing relative uncertainty for properties inferred indirectly via covariance (e.g., available phosphorus, potassium). Despite significant local and regional progress, the absence of a standardized pan-African spectral library and the intractable transferability problem remain formidable barriers. Future research must pivot decisively toward imaging spectroscopy and satellite platforms, mitigating PLSR dominance through systematic adoption of ensemble methods, transfer learning, and model harmonization frameworks to fully operationalize these technologies in support of Africa’s sustainable development goals. Full article

Spent coffee grounds (SCGs) are one of the most abundant agro-industrial by-products worldwide, with 650 kg generated per ton of green coffee processed, corresponding to an estimated global production of 6.7 million tons in 2022/2023. Improper disposal of SCG raises environmental concerns, while their reuse offers opportunities for sustainable resource management and circular economy strategies. This review examines SCG valorization by addressing their chemical composition, functional properties, and key applications in sectors such as food, agriculture, environmental remediation, bioenergy, and selected industrial fields, including pharmaceuticals, cosmetics, construction materials, and functional devices. In this context, it discusses technological approaches, performance outcomes, and implementation considerations, emphasizing the multifunctional potential of SCGs as a renewable feedstock capable of reducing waste, improving resource efficiency, and generating economic value. By consolidating the current state of knowledge and exploring diverse valorization pathways, this work frames SCG utilization within a circular bioeconomy framework and highlights how innovative applications can transform this widely available waste into sustainable and economically valuable products. Full article

Background/Objectives: Fruit and vegetable (FV) availability/accessibility are associated with increased intake of FVs and are important determinants of intake. The aim of this analysis was to evaluate the pre-post changes in an FV accessibility intervention and examine cross-sectional associations between accessibility domains and diet quality categories at pre- and post-intervention. Methods: Thirty parent–child dyads (mean age = 41.2 ± 4.7; 9.2 ± 1.9) completed an 8-week pre-post intervention. Assessments included perceptions of accessibility, the Home Food Inventory with added accessibility domains, and three-day diet records used to calculate HEI-2020 scores. Stuart–Maxwell tests were used to evaluate changes in categorical responses, paired t-tests assessed pre-post changes, and independent t-tests compared accessibility by HEI category. Results: Parents reported a perceived increase in frequency of having the form of FVs prepared/ready for use (p = 0.034). No significant pre–post changes were observed in objective FV availability/accessibility domains, FV intake, or HEI scores for children and parents. Exploratory analyses showed that children and parents with HEI-total scores above national averages had higher mean FV location and visibility, with post-intervention visibility being significantly associated with higher HEI among children (p = 0.048) and location being significantly associated with higher HEI among parents at pre- (p = 0.033) and post-intervention (p = 0.046). Conclusions: The FV accessibility intervention did not significantly improve objective HFE accessibility or diet quality in this small sample. Exploratory findings suggest that FV accessibility domains may be associated with diet quality; however, these observations are preliminary. Larger and longer-term studies are needed to determine whether modifying FV accessibility can meaningfully improve children’s dietary intake. Full article

To combat climate change, farmers want to develop sustainable agriculture that enhances food production while strengthening their capacity to cope with extreme weather events and pest and disease pressures. Promoting agroecological farming practices is a promising approach in enhancing sustainability and strengthening the climate-resilient farming systems. Recent research often overlooks to what extent the agroecological farming practices (AFP) provide a measurable advantage over non-AFP methods under increasing environmental challenges. In this regard, this study compares the extent of climate resilience between AFP mango-based farming systems and non-AFP mango-based farming systems in southern Ethiopia. AFP adopters applied ecological principles like intercropping, integrated pest management, agroforestry, canopy management, varietal diversity, and water and soil preservation to enhance biodiversity and soil health, and boost productivity and ecosystem services. The study employed a mixed-method design, drawing on the data from 395 selected households. The resilience of AFP and non-AFP farming systems was assessed by computing the 13 agroecosystem indicators of climate resilience using the Self-evaluation and Holistic Assessment of Climate Resilience of Farmers and Pastoralists (SHARP+) tool. Households in AFP mango-based farming system demonstrated greater diversification in agricultural production system compared to those in non-AFP mango farming system. The analysis of climate resilience indicators showed that the mango production systems under the AFP were more climate-robust than their conventional systems. Both the compound resilience score and the household resilience index showed that the mango farming systems under AFP substantially enhanced climate resilience. Hence, coordinated supports from the extension services, NGOs, and researchers are needed to scale up these benefits of AFP. Strengthening the AFP mango farming requires addressing the key barriers such as market access, input availability, and crop diversification strategies. This paper identifies important avenues for further AFP research in Sub-Saharan African countries. Full article

Global climate change is rapid and poses an alarming threat to agricultural production, significantly impacting economies. Modern agriculture has strongly emphasized improving nutrient availability in crops to address rising malnutrition and contribute to global food security. However, abiotic stresses, including warmer temperatures, drought, waterlogging stress, and elevated CO_2, have critical direct and indirect effects on nutrient availability in plants. This systematic review was conducted in accordance with the PRISMA guidelines. The literature survey followed a time period of 2–5 months, during which the conceptualization, analysis, writing, and editing of the article were conducted. In the present era, it is essential to adopt measures to improve the nutritional value [enhance Nutrient Use Efficiency (NUE)] and nutrient management of plant-based foods. Plant-associated microbiomes have co-evolved with their plant counterparts and perform essential functions in nutrient acquisition, including microbial sensing and cross-talk with the plant host, nutrient uptake and sharing, and signaling mechanisms. In natural and agricultural ecosystems, plant microbiomes offer major opportunities and can be harnessed to sustainably supply essential plant nutrients and improve NUE in crops of global importance. Crop-associated microbiomes can be precisely tailored to achieve targeted outcomes, enhancing nutrient acquisition and utilization via microbiome engineering. However, bridging knowledge gaps, understanding microbial colonization, plant–microbiome dynamics, and adopting precise editing approaches are crucial to boost sustainable outcomes and crop productivity. By elucidating plant microbiome crosstalk and microbe–microbe signaling, a better understanding of microbe-mediated nutrient acquisition in plants can be achieved, defining key implications in global food security. Full article

Agriculture in the Pacific is driven primarily by small-scale private farmers, many of whom do not have access to soil testing services or advice, nor the means to interpret analytical results into soil management and agronomic recommendations. Soil degradation through the process of acidification poses a significant risk to food and income security as it directly threatens crop productivity. The nutritional quality of food crops may also be affected through sub-optimal nutrient uptake by plants and nutrient imbalances. The dataset reported here provides a useful platform for the development of a decision-support tool (DST) that will assist Fiji farmers in understanding and managing soil pH and soil acidity. The DST will enable making informed decisions about liming to help correct soil pH. To support this development, historical soil pH data available from the Pacific Soils Portal were combined with updated analyses of agricultural soils from 17 locations in Viti Levu Island (Fiji) collected during a field campaign undertaken in August 2025. The soils were sampled at two depth intervals (0–15 and 15–30 cm) and analyzed for pH using a variety of methods. These methods included direct field measurements using a portable pH-meter as well as traditional laboratory determinations. Of the soils sampled, it was found that most soils exhibited pH levels below 7, which were observed for both depth intervals. Across all samples taken in 2025, it was found that 54.3% of them had soil pH < 5, 38.6% had soil pH between 5 and 6, and 7.1% had pH > 6 (based on soil pH_1:5 soil-to-water method). Depending upon specific land uses, climate and cropping intensity, it was recommended that routine liming be built into soil fertility management programs to help farmers overcome soil acidity-related constraints to production. Liming frequency, timing of application and application rate will need to be determined for specific soil and cropping situations; however, it was suggested that soil pH was not changed by more than 1 unit each time lime was applied. Such an approach should reduce the risk of soil organic matter loss through accelerated mineralization, which would be challenging to restore in that environment if soils remained under continuous cropping. The analytical information contained in this article expanded and updated the datasets available in the Pacific Soils Portal. Furthermore, this work provided an opportunity to build analytical expertise in aspects of soil chemistry at local organizations to support academic and extension activities as well as the ongoing development of the Pacific Soils Portal. Full article