Search Results (2,703)

Elucidating the molecular mechanisms underlying beef quality differences is crucial for precision breeding of high-quality cattle. In this study, we first characterized the myofibrillar morphology of high-quality (H group) and low-quality (L group) beef samples using hematoxylin–eosin (HE) staining. Transcriptomic and metabolomic analyses were then conducted to reveal the molecular regulatory basis of quality variation. HE staining revealed highly significant differences in muscle fiber area and diameter between H and L groups (p < 0.01), along with significant differences in muscle fiber density (p < 0.05), but no significant differences in muscle fiber perimeter. Furthermore, by focusing on five core metabolic pathways shared across the transcriptome and metabolome datasets, 30 differentially expressed genes (DEGs) and 14 differentially accumulated metabolites (DAMs) were identified. Pearson correlation analysis revealed synergistic regulation between DEGs and DAMs: AMPD2 modulates umami flavor by regulating inosine accumulation via the purine metabolism pathway; ACOX3 promotes unsaturated fatty acid synthesis and intramuscular fat deposition through carbohydrate metabolism; genes in the glycolysis/gluconeogenesis pathway maintain post-slaughter muscle pH homeostasis, thereby influencing beef tenderness. Collectively, this study integrates morphological and molecular evidence to elucidate the multi-level basis of beef quality formation, providing key candidate genes, metabolites, and pathways for molecular breeding. These findings offer comprehensive theoretical and technical support for the sustainable development of the premium beef industry. Full article

This article examines how hybrid polypropylene fibers of three different lengths affect the mechanical and fracture properties of lightweight structural concrete with lightweight ceramic aggregate. Four mixtures were produced: a reference lightweight concrete and three fiber-reinforced variants with total dosages of 3, 6, and 9 kg/m³ in a fixed length ratio of 4:1:1. Standard tests determined the bulk density, cube compressive strength, splitting tensile strength, modulus of elasticity, and fracture parameters using a three-point bend test. Compared to the reference concrete, the fibers did not significantly change the compressive strength but consistently increased the tensile strength and energy absorption after cracking. The highest fracture energy and toughness were obtained at the highest dosage, while excessive fiber content reduced the static compressive modulus. Full article

Background and Objectives: Extracellular matrix (ECM) and collagen remodeling contribute to chronic kidney disease (CKD) progression and vascular access dysfunction. Conventional histological techniques rely on staining and provide limited sensitivity for detecting early or subtle ECM alterations. Nonlinear optical imaging modalities, including second-harmonic generation (SHG), third-harmonic generation (THG), and multiphoton fluorescence (MPF) microscopy, enable label-free, high-resolution visualization of fibrillar collagen and may offer additional structural information. This study aimed to evaluate the added value of nonlinear imaging beyond conventional histology for assessing ECM remodeling in renal and vascular tissues. Materials and Methods: A systematic literature review was conducted in accordance with the PRISMA 2020 guidelines. PubMed and Web of Science were searched for studies published between 1 January 2015, and 4 April 2025, investigating ECM or collagen remodeling in renal or vascular tissues using SHG, THG, or MPF microscopy. After screening 115 records, 10 studies were included in the qualitative synthesis. In addition, representative SHG, THG, and MPF images of excised human arteriovenous fistula (AVF) tissue were acquired as illustrative feasibility examples to demonstrate the application of these imaging modalities. The use of human tissue was approved by the Vilnius Regional Biomedical Research Ethics Committee (approval No. 2022/6-1443-917). Results: The included studies demonstrated that nonlinear microscopy enables label-free assessment of collagen density, organization, and fiber orientation. SHG imaging differentiated healthy from diseased tissues and has been reported to support fibrosis assessment and staging in preclinical and selected clinical studies and revealed microstructural remodeling patterns not readily detected by conventional histology. The illustrative AVF images demonstrated collagen disorganization consistent with patterns reported in the reviewed literature and are presented solely to demonstrate imaging feasibility, without implying disease phenotype or clinical outcome associations. Conclusions: Nonlinear optical microscopy provides complementary structural information on ECM organization that is not accessible with standard histological techniques. Further validation and methodological standardization are required to support its broader application in clinical nephrology and vascular medicine. Full article

Data on microplastic contamination in pristine caves are rare, thus limiting our understanding of its pervasiveness in intact underground ecosystems. Here, we quantified microplastics in sediments from two newly discovered, extremely remote caves (Maucci and Luftloch) and compared them with a frequently visited cave (Trebiciano), all three of which are hydraulically connected to the Reka/Timavo River in the Classic Karst (NE Italy). Sediment samples were collected along river-to-slope transects and analyzed for microplastics using density separation and μFT-IR spectroscopy. Average contamination levels were comparable across caves, ranging from 84.7 to 105.9 items kg⁻¹ (dry weight). Fibers and fragments dominated, with similar polymer spectra across sites—polypropylene (PP, 29–42%), polyethylene (PE, 19–27%), and polyethylene terephthalate (PET, 33–46%). Microplastic abundance systematically increased with elevation, up to ~4–12× from river-proximal to high-bench sediments. Polymer-resolved trends reflected density-coupled, flood-driven sorting with low-density PP and PE accumulated on higher benches and denser PET depleted aloft, indicating slackwater retention at flood crests and re-entrainment of lower benches during recession. These findings suggest that indirect riverine inputs of microplastics outweigh direct human contamination and provide the first baseline for pristine Timavo caves—serving as reference sites for background microplastic levels in the Classic Karst and similar karst systems worldwide. Full article

The pursuit of alternatives to nonrenewable materials has stimulated the development of sustainable materials with improved performance, particularly polymer composites reinforced with plant-based fibers. In this study, eucalyptus fibers were thermally treated and evaluated as eco-friendly reinforcements for polyester composites, aiming to enhance their physical and mechanical properties. The fibers were subjected to heat treatments between 140 and 230 °C in a Macro-ATG oven, followed by analyses of anatomical characteristics and chemical composition. Composites containing 25% fiber reinforcement were produced using an orthophthalic unsaturated polyester matrix catalyzed with methyl ethyl ketone peroxide, with untreated fibers used as references. Thermal treatment induced significant modifications in fiber morphology and composition, including increases in cell wall fraction at 170 and 200 °C and higher cellulose contents at 140 and 170 °C. Mechanical performance was assessed through tensile, flexural (modulus of rupture—MOR), modulus of elasticity (E_B), and impact tests. Composites reinforced with heat-treated fibers exhibited lower apparent density and, notably, those treated at 230 °C showed markedly reduced water absorption and enhanced tensile strength compared with the control. Overall, treatment at 230 °C proved most effective, highlighting the potential of thermally modified eucalyptus fibers as viable reinforcements for high-performance, bio-based polymer composites. Full article

Background: One method for the radiation therapy of rectal cancer is to set patients supine and treat them with volumetric modulated arc therapy (VMAT). The posterior skin dose is of concern due to undesirable bolusing from mounting surfaces the patient lays upon, namely the carbon fiber couch (CFC). The posterior skin dose may be mitigated by positioning the patient on top of a low-density material that separates the patient from the CFC. Purpose: Our objective was to determine the reduction in the posterior surface dose when a mattress or foam board is used to prop the patient away from the CFC. Materials and Methods: Three clinical rectal cancer patient VMAT plans were selected. A solid water phantom with optically stimulated luminescence dosimeters (OSLDs) placed at the posterior surface was mounted using three setups: directly on the CFC, with a mattress on the CFC, and with a 10 cm thick foam board on the CFC. The three VMAT plans were delivered to this phantom, with OSLDs measuring the posterior surface dose with each setup. In the treatment planning system (TPS), the CFC only, mattress, and foam board setups were simulated on the patient’s anatomy with posterior surface doses reported. Results: The OSLD measurements in the phantom showed that the mattress reduced the posterior surface dose on average by 1.3%, and the foam board reduced the dose by 8.3%. The TPS estimates demonstrated that, on average, the mattress reduced the surface dose by 15.8%, and the foam board reduced the dose by 33.0%. It is likely that the TPS had limitations accurately modeling the surface dose, so OSLD measurements were closer to clinical reality. Conclusions: The mattress does not reduce the posterior skin dose enough to warrant its use as a skin sparing device. The CFC produces a bolusing effect that can be reduced by separating the patient from the CFC with a 10 cm thick foam board. Full article

Alpacas thrive in Andean ecosystems, efficiently converting natural pasture into products such as fiber and meat, making their breeding a production alternative in Guamote. Intensive grazing and the shift in the spatial distribution of plants due to climate change negatively impact the moorlands. In this context, this study analyzed the influence of floristic composition on the productivity and quality of natural pastures. The methodology included a floristic inventory in a sample of 98 cells in four communities, collecting flora data using the Parker method to measure species composition, density, and cover. In addition, soil fertility and nutritional quality of desirable pastures were assessed through physical and chemical analyses. Principal component and cluster analyses were then applied to correlate the variables. The results showed 26 species, with Poaceae and Asteraceae standing out as dominant and abundant. Tablillas and Pull Quishuar stood out for their productivity and carrying capacity (4.83 t/ha), while Galte Bisñag showed high protein and plant vitality in their pastures. Component 1 stood out for its high production (3.71 t/ha) and carrying capacity in fertile soils; Axis 2 linked Galte Bisñag with high nutritional quality and vegetation cover, while Axis 3 related Asaraty with compacted soils and an intermediate balance. The direct influence between floral species and the productivity of natural pastures leads to the exploration and implementation of measures for sustainable grazing. Full article

Background/Objectives: End-stage renal disease (ESRD) is characterized by profound and progressive microvascular dysfunction that contributes significantly to systemic morbidity. Because the retinal and renal microcirculations share structural and physiological similarities, optical coherence tomography (OCT) and OCT angiography (OCTA) have emerged as promising tools for detecting ocular microvascular changes that may parallel systemic vascular injury. This systematic review aimed to consolidate evidence on chronic retinal and choroidal alterations in ESRD as assessed by OCT and OCTA. Methods: A systematic search of PubMed/MEDLINE (inception–June 2025) was performed using combinations of terms related to OCT, OCTA, ESRD, and hemodialysis. After removing duplicates and screening titles, abstracts, and full texts, we included clinical studies involving adults with ESRD or undergoing dialysis that reported chronic or baseline OCT/OCTA findings. Non-English publications, editorials, conference abstracts, case reports, and studies limited to acute pre-/post-dialysis changes were excluded. Seventeen studies met eligibility criteria. Acute findings were summarized narratively only when no chronic data existed for a specific parameter but were not incorporated into the primary synthesis. Results: Across eligible studies, chronic structural and perfusion abnormalities were consistently reported, including thinning of the retinal nerve fiber and ganglion cell layers, reduced macular and peripapillary vascular densities, enlarged foveal avascular zones, and decreased choroidal thickness. These alterations aligned with markers of disease severity and systemic microvascular burden. Conclusions: Retinal imaging reveals reproducible chronic microvascular changes in ESRD and may serve as an accessible adjunct for systemic vascular assessment. We highlight the potential significance of retinal vascular screening in this population and the need for more standardized imaging protocols to support the effective integration of retinal biomarkers into CKD diagnostic and monitoring strategies. Full article

To meet the requirements of next-generation spacecraft thermal protection systems for lightweight materials with high strength, effective thermal insulation, and superior ablation resistance, a novel POSS-modified phenolic aerogel/quartz fiber composite (POSS-PR/QF) was developed using a thiol–ene click reaction combined with a sol–gel process. Covalent incorporation of polyhedral oligomeric silsesquioxanes (POSS) into the phenolic matrix effectively eliminates nanoparticle aggregation and improves interfacial compatibility. As a result, the modified resin is suitable for resin transfer molding (RTM) processes. The resulting composite exhibited an aerogel-like porous structure with enhanced crosslinking density, thermal stability, and oxidation resistance. At 7.5 wt% POSS loading, the composite achieved low density (~0.7 g·cm⁻³) and outstanding mechanical properties, with tensile, flexural, compressive, and interlaminar shear strengths increased by 114%, 79%, 29%, and 104%, respectively. Its thermal conductivity (0.0619 W/(m·K)) and ablation rates were also markedly reduced. Mechanistic studies revealed that POSS undergoes in situ ceramification to form SiO₂ and SiC phases, which create a dense protective barrier. In addition, this ceramification process promotes char graphitization, thereby enhancing oxidation resistance and thermal insulation. This work provides a promising approach for designing lightweight, high-performance, and multifunctional thermal protection materials for aerospace applications. Full article

The lightning strike protection (LSP) performance of nickel-coated carbon fiber nonwoven veils (NiCVs) with varying areal densities, integrated onto the surface of CFRP laminates, was evaluated through simulated lightning strike tests. Post-strike damage was evaluated through visual inspection, non-destructive ultrasonic testing, residual strength measurements, and microstructural examinations. Results indicated that the protection effectiveness improved with increasing NiCV areal density. The laminate with a 68 g/m² NiCV layer showed substantially reduced damage—its damage volume, damage area, and maximum damage depth decreased to 18%, 40%, and 51% of those of the control laminate—and it retained 95% of the reference compression strength, demonstrating the strong post-strike protection capability of this lightweight veil. A detailed analysis suggested that the NiCV LSP performance may arise from a mechanism involving high electrical conductivity, a thermally stable coated-fiber skeleton, as well as a distributed nonwoven network architecture. These results highlight NiCV as a promising functional approach for enhancing the lightning strike protection of CFRP aerostructures. Full article

In this study, X70 line pipe steels were subjected to different hot rolling treatments under three conditions with varying roughing (R) and finishing (F) reductions while maintaining the same total reduction to investigate the effect on drop weight tear test (DWTT) toughness and hydrogen-induced damage as assessed through electrochemical charging. Scanning Electron Microscope (SEM) images were used to analyze microstructure phases and their volume fractions, while Electron Backscatter Diffraction (EBSD) provided quantitative microscopy, and X-ray analysis examined crystallographic texture. Although all steels exhibited similar microstructure phases, the effective grain size and morphology varied slightly across the thickness. As these variations were minor, the focus shifted to other microstructural features such as textural characteristics. Overall, the steel with the medium R/F reduction demonstrated improved DWTT performance and greater hydrogen cracking and blistering resistance. This was attributed to stronger Transformed Brass (TBr) and Transformed Copper (TC) components, weaker Rotated-Cube (RC) texture, and lower Kernel Average Misorientation (KAM) values. Across the three steels in this work, this study demonstrates that increased fraction of blocky austenite/martensite as secondary phases, high geometrically necessary dislocation (GND) density, and RC texture negatively affect both DWTT and hydrogen damage resistance, whereas gamma (γ)-fiber and {332}<113> textures have positive effects. Improving these metallurgical factors can therefore boost toughness and reduce hydrogen-induced damage in line-pipe steels. Full article

The evolution of next-generation urban infrastructure necessitates the deployment of intelligent pavement systems capable of real-time data acquisition, adaptive response, and predictive analytics. This article presents the design, implementation, and performance evaluation of the smart pavement system incorporating multimodal embedded sensors for traffic density analysis, structural health monitoring, and environmental surveillance. SPS integrates piezoelectric transducers, micro-electro-mechanical system accelerometers, inductive loop coils, fiber Bragg grating (FBG) sensors, and capacitive moisture and temperature sensors within the asphalt and sub-base layers, forming a distributed sensor network that interfaces with an edge-AI-enabled data acquisition and control module. Each sensor node performs localized pre-processing using low-power microcontrollers and transmits spatiotemporal data to a centralized IoT gateway over an adaptive mesh topology via long-range wide-area network or 5G-Vehicle-to-Everything protocols. Data fusion algorithms employing Kalman filters, sensor drift compensation models, and deep convolutional recurrent neural networks enable accurate classification of vehicular loads, traffic, and anomaly detection. Additionally, the system supports real-time air pollutant detection (e.g., NO₂, CO, and PM2.5) using embedded electrochemical and optical gas sensors linked to mobile roadside units. Field deployments on a 1.2 km highway testbed demonstrate the system’s capability to achieve 95.7% classification accuracy for vehicle type recognition, ±1.5 mm resolution in rut depth measurement, and ±0.2 °C thermal sensitivity across dynamic weather conditions. Predictive analytics driven by long short-term memory networks yield a 21.4% improvement in maintenance planning accuracy, significantly reducing unplanned downtimes and repair costs. The architecture also supports vehicle-to-infrastructure feedback loops for adaptive traffic signal control and incident response. The proposed SPS architecture demonstrates a scalable and resilient framework for cyber-physical infrastructure, paving the way for smart cities that are responsive, efficient, and sustainable. Full article

The thermally efficient and lightweight TRC/CLCi composite panels for functional and smart building envelopes, funded by the iclimabuilt project (Grant Agreement no. 952886), offer innovative solutions to sustainably address common failure risks in facade systems. This work specifically emphasizes strategies for mitigating structural, thermal, and fire-related failures through targeted material selection, advanced design methodologies, and rigorous validation protocols. To effectively mitigate structural failures, high-pressure concrete (HPC) reinforced with carbon fibers is utilized, significantly enhancing tensile strength, reducing susceptibility to cracking, and improving overall durability. To counteract thermal bridging—a critical failure mode compromising energy efficiency and structural integrity—the panels employ specially designed glass-fiber reinforced pins connecting HPC outer layers through the cellular lightweight concrete (CLC) insulation core that has a density of around 70 kg/m³ and a thermal conductivity in the range 35 mW/m∙K comparable to those of expanded polystyrene and Rockwool. These connectors ensure effective load transfer and maintain optimal thermal performance. A central focus of the failure mitigation strategy is robust fire behavior. The developed panels undergo rigorous standardized fire tests, achieving an exceptional reaction to fire classification of A2. This outcome confirms that HPC layers maintain structural stability and integrity even under prolonged fire exposure, effectively preventing catastrophic failures and ensuring occupant safety. In conclusion, this work highlights explicit failure mitigation strategies—reinforced concrete materials for structural stability, specialized glass-fiber connectors to prevent thermal bridging, rigorous fire behavior protocols, and comprehensive thermal performance validation—to produce a facade system that is robust, energy-efficient, fire-safe, and sustainable for modern buildings. Full article

The steel industry contributes to approximately 7%–9% of global anthropogenic CO_2(g) emissions, with traditional blast furnace–basic oxygen furnace (BF–BOF) routes emitting up to 1.8 t_CO2 per ton of steel. In contrast, Electric Arc Furnace (EAF) steelmaking, especially when integrated with hydrogen direct-reduced iron (DRI), can reduce emissions by over 40%, positioning EAFs as a key enabler of low-carbon metallurgy. However, despite its lower direct emissions, the EAF process still depends on fossil carbon sources for slag foaming and FeO reduction, which are essential for arc stability and energy efficiency. Slag foaming plays a critical role in controlling the thermal efficiency of the EAF by shielding the electric arc, reducing radiative heat losses, and stabilizing the arc’s behavior. This review examines the mechanisms of slag foaming, discussed through empirical models that consider the foaming index (Σ) and slag foaming rate as critical parameters, and highlights the influence of physical properties such as slag viscosity, surface tension, and density on gas bubble retention. Also, the work embraces the potential use of alternative carbon sources including biochar, biomass, and waste-derived materials such as plastics and rubber to replace fossil-based reductants and foaming agents in EAF operations. Finally, it discusses the use of new materials with a biological base, such as nanocellulose, to serve as reactive templates for producing nanohybrid materials, containing both oxides, which can contribute to slag basicity (MgO and/or CaO, for example), together with a reactive carbonaceous phase, derived from the organic fiber’s thermal degradation, which could contribute to slag foaming, and could replace part of the fossil fuel charge to be employed in the EAF process. In this context, the development and characterization of renewable carbonaceous materials capable of simultaneously reducing FeO and promoting slag foaming are essential to achieving net-zero steel production and enhancing the sustainability of EAF-based steelmaking. Full article

Magnetorheological (MR) materials, with the ability of vectorial response, offer exciting opportunities for next-generation wearables and soft robotic systems. Although some existing MR materials and fiber designs can produce directional responses, they typically rely on strategies—such as hard-magnetic loading or pre-magnetization—that constrain safety and large-scale manufacturability. This Communication highlights a paradigm-shifting advance reported by Pu et al., that a soft-magnetic fibrous architecture achieves genuine vector-stimuli-responsiveness under low, safe magnetic fields without pre-magnetization. We articulate the great breakthrough of this work through a hierarchical design framework, demonstrating how the synergistic innovation at the material (magnetic dipole aligned in low-density polyethylene), fiber (drawing-induced magnetic easy axis), yarn (twist-induced cooperative effects), and fabric (vertical or horizontal magnetic field response capability) levels collectively resolves the longstanding trade-offs between performance, manufacturability, and safety. As a result, this strategy demonstrates strong universality in terms of materials, although only the carbonyl iron particles were used. This approach not only enables programmable bending, stiffening, shear, and compression in textiles but also establishes a versatile platform for magneto-programmable systems. Furthermore, we delineate the critical challenges and future trajectories—from theoretical modeling and integration of complementary stimuli to the development of three-dimensional textile architectures—that this new platform opens for the fields of haptics, soft robotics, and adaptive wearables. Full article