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35 pages, 2859 KB  
Article
Laser Linewidth Effects in Continuous-Variable QKD: Simulation-Based Analysis and Optimization Guidelines for Defense-Grade Secure System
by Seyed Saman Mahjour and Fernando M. Araújo-Moreira
Photonics 2026, 13(5), 432; https://doi.org/10.3390/photonics13050432 (registering DOI) - 27 Apr 2026
Abstract
Continuous-Variable Quantum Key Distribution (CV-QKD) offers practical advantages for secure communication, but laser linewidth-induced phase noise remains a critical performance limitation. This work presents a comprehensive simulation-based analysis quantifying the impact of laser linewidth on secret key rate (SKR) in Gaussian-modulated coherent-state CV-QKD [...] Read more.
Continuous-Variable Quantum Key Distribution (CV-QKD) offers practical advantages for secure communication, but laser linewidth-induced phase noise remains a critical performance limitation. This work presents a comprehensive simulation-based analysis quantifying the impact of laser linewidth on secret key rate (SKR) in Gaussian-modulated coherent-state CV-QKD systems. We develop a detailed noise model incorporating detector electronics, Raman scattering, phase recovery, ADC quantization, and laser relative intensity noise. Through systematic parameter sweeps spanning linewidths from 10 Hz to 250 kHz, modulation variances from 1 to 20 SNU, and fiber distances up to 100 km, we identify three distinct operational regimes and optimization strategies for both transmitted local oscillator (TLO) and local–local oscillator (LLO) configurations under homodyne and heterodyne detection. Results show that metropolitan-scale links (50 km) require linewidths below 5 kHz to maintain secure operation, with performance decreasing beyond 25 kHz. We demonstrate that modulation variance must be jointly optimized with laser quality, with optimal values decreasing from 3–4 SNU at narrow linewidths to 2–2.5 SNU at moderate linewidths. The analysis reveals asymmetric sensitivity in LLO systems where local oscillator linewidth degrades performance more strongly than signal laser linewidth. These quantitative findings provide practical design guidelines for achieving secure CV-QKD operation over metropolitan distances with realistic hardware constraints, supporting deployment of defense-grade quantum communication networks. Full article
(This article belongs to the Special Issue Quantum Optics: Communication, Sensing, Computing, and Simulation)
17 pages, 3790 KB  
Article
Characterization of the Fiber Protein C-Terminal Domain from Klebsiella pneumoniae Phage KlebP_144 and Evaluation of Its Anti-Capsular Activity
by Bogdana I. Kravchuk, Natalia N. Golosova, Ekaterina A. Kondakova, Yana A. Khlusevich, Vyacheslav I. Yakubovskij, Margarita I. Arisova, Yuliya N. Kozlova, Nina V. Tikunova and Andrey L. Matveev
Int. J. Mol. Sci. 2026, 27(9), 3883; https://doi.org/10.3390/ijms27093883 (registering DOI) - 27 Apr 2026
Abstract
Klebsiella pneumoniae, a member of the ESKAPEE group of priority pathogens, has become one of the most challenging bacterial pathogens in modern clinical practice, largely due to its multidrug resistance and the immune-evasive effect of its capsular polysaccharide (CPS). Phage-encoded depolymerases, which [...] Read more.
Klebsiella pneumoniae, a member of the ESKAPEE group of priority pathogens, has become one of the most challenging bacterial pathogens in modern clinical practice, largely due to its multidrug resistance and the immune-evasive effect of its capsular polysaccharide (CPS). Phage-encoded depolymerases, which selectively degrade the capsular polysaccharide, have emerged as promising antimicrobial agents capable of restoring bacterial susceptibility to both immune clearance and phage infection. The fragment corresponding to the C-terminal region of a putative depolymerase of bacteriophage KlebP_144, namely DepKP144ΔC, was cloned, expressed in E. coli, and purified using immobilized metal affinity chromatography. DepKP144ΔC displays an enzymatic activity against capsular polysaccharides of 100% K1 capsular-type strains and 85% K2 capsular-type strains, including classical and hypervirulent isolates. It was demonstrated that this protein is capable of inhibiting K. pneumoniae biofilm formation, but it is unable to disrupt mature biofilms. In vivo experiments using a murine K. pneumoniae infection model further confirmed its therapeutic potential: treatment with DepKP144ΔC improved survival rate in mice infected with K2-type K. pneumoniae, indicating significant attenuation of bacterial virulence. Therefore, these results demonstrate the potential role of the C-terminal domain of the bacteriophage KP144 tail-fiber protein in phage entry and show that its carbohydrate-recognition motifs possess enzymatic activity against the Klebsiella capsular polysaccharides. Full article
(This article belongs to the Special Issue New Insight into Bacteriophage and Their Potential Applications)
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35 pages, 19590 KB  
Review
Research Status, Challenges and Future Perspectives of Geological Hazard Monitoring Methods in Mining Areas
by Yanjun Zhang, Yue Sun, Yueguan Yan, Shengliang Wang and Lina Ge
Remote Sens. 2026, 18(9), 1333; https://doi.org/10.3390/rs18091333 (registering DOI) - 27 Apr 2026
Abstract
Geological hazards induced by large-scale and high-intensity mining activities worldwide are primary drivers of regional ecological degradation and pose significant threats to human safety and property. To construct efficient monitoring systems and enhance early warning capabilities, it is essential to clarify the formation [...] Read more.
Geological hazards induced by large-scale and high-intensity mining activities worldwide are primary drivers of regional ecological degradation and pose significant threats to human safety and property. To construct efficient monitoring systems and enhance early warning capabilities, it is essential to clarify the formation mechanisms of various hazards and the suitability of corresponding technologies. Focusing on four typical geological hazards prevalent in mining areas (surface subsidence, ground fissures, landslides, collapses, and sinkholes), this paper characterizes their specific features and monitoring requirements. It systematically analyzes the physical principles, accuracy levels, and technical advantages and limitations of ground-based, aerial, and spaceborne monitoring, as well as multi-source remote sensing data fusion and emerging technologies (e.g., distributed optical fiber, light detection and range, microseismical monitoring, and deep learning). Utilizing case studies from an open-pit coal mine in Turkey and a loess gully mining area in China, the paper evaluates the effectiveness of methods like multi-temporal InSAR and UAV photogrammetry in identifying the evolution of these hazards. The findings indicate that the technological framework for mining area monitoring is transitioning from single-method approaches to integrated systems. However, given the complex mining environment, several bottleneck challenges remain, including single data dimensions, the limited environmental adaptability of aerospace remote sensing, insufficient stability of deep monitoring equipment, and weak anti-interference capabilities under extreme operating conditions. Consequently, this paper proposes that future innovations in geological hazard monitoring in mining areas will focus on multi-platform hierarchical collaboration, the development of multi-parameter fusion early warning criteria, and the construction of digital and visual platforms. Constructing a comprehensive monitoring system characterized by multi-scale collaboration and dynamic prediction capabilities is vital for improving safety standards in mining areas and achieving coordinated development between resource exploitation and environmental protection. The findings provide a theoretical foundation for the precise prevention and control of mining hazards, as well as for land ecological restoration. Full article
(This article belongs to the Special Issue Applications of Photogrammetry and Lidar Techniques in Mining Areas)
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32 pages, 2930 KB  
Review
Review of the Phosphorylation of Lignocellulosic Fibers: Reaction Products, Characterization, and Potential Applications
by Lahbib Abenghal, Dan Belosinschi, Hamid Lamoudan, Aleksandra Mikhailidi and François Brouillette
Fibers 2026, 14(5), 50; https://doi.org/10.3390/fib14050050 (registering DOI) - 27 Apr 2026
Abstract
Natural fibers are among the most extensively exploited bio-based materials in industry due to their abundance, affordability, and biodegradability. However, their intrinsic properties often require improvement through chemical, mechanical, or enzymatic treatments to expand their applications. Phosphorylation is a highly effective chemical modification [...] Read more.
Natural fibers are among the most extensively exploited bio-based materials in industry due to their abundance, affordability, and biodegradability. However, their intrinsic properties often require improvement through chemical, mechanical, or enzymatic treatments to expand their applications. Phosphorylation is a highly effective chemical modification that enables the covalent grafting of phosphate groups onto the fiber backbone. These functionalities enhance hydrophilicity, anionic charge density, swelling capacity, and water uptake, while significantly improving flame-retardant performance. In addition, phosphorylation can reduce energy consumption and production costs in the manufacture of functionalized micro- and nanofibrillated fibers, as the increased swelling facilitates fibrillation. Consequently, phosphorylated fibers are suitable for water treatment, biomedical devices, construction materials, and other advanced materials. Dozens of reagents and various synthetic routes have been explored to perform this reaction, each producing materials with distinct properties. Phosphorus content remains the primary parameter used to assess modification efficiency. This literature review examines existing phosphorylation methods, including reagents, substrates, and characterization techniques, and discusses applications such as flame retardancy, thermal insulation, ion exchange, energy storage, electrodes, and battery recycling. It also briefly addresses key challenges, including limited hydroxyl accessibility, control of the degree of substitution, potential cellulose degradation, and scalability constraints. Full article
(This article belongs to the Collection Review Papers of Fibers)
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20 pages, 909 KB  
Article
Effects of NSP Enzymes and Candida tropicalis KKU20 on the Nutritional and Fermentation Characteristics of Corn Dust
by Tanapon Mattayaruk, Yotsapon Yangngam, Seangla Cheas, Chanon Suntara, Metha Wanapat, Chanadol Supapong, Areerat Lunpha, Ruangyote Pilajun, Payungsuk Intawicha and Anusorn Cherdthong
Fermentation 2026, 12(5), 213; https://doi.org/10.3390/fermentation12050213 (registering DOI) - 27 Apr 2026
Abstract
Corn dust is an abundant agro-industrial by-product with potential as an alternative energy source. Its use in animal feeding, however, is restricted by high fiber content and low digestibility. This study evaluated the effects of non-starch polysaccharide (NSP) enzymes and yeast (Candida [...] Read more.
Corn dust is an abundant agro-industrial by-product with potential as an alternative energy source. Its use in animal feeding, however, is restricted by high fiber content and low digestibility. This study evaluated the effects of non-starch polysaccharide (NSP) enzymes and yeast (Candida tropicalis KKU20) on the chemical composition, fermentation characteristics, and microbial populations of fermented corn dust. The experiment was conducted using a completely randomized design with a 3 × 2 factorial arrangement plus an additional control treatment. Factor A consisted of three levels of enzyme supplementation (0.02%, 0.04%, and 0.06% of dry matter), and Factor B consisted of yeast supplementation (without yeast or with C. tropicalis KKU20, approximately 1 × 1013 cells/g of inoculum). The control treatment consisted of fermented corn dust without enzyme or yeast supplementation. Samples were fermented for 15 days prior to analysis. Yeast inoculation increased crude protein and non-fiber carbohydrate contents while reducing neutral detergent fiber, acid detergent fiber, and acid detergent lignin (p < 0.05). Significant enzyme × yeast interactions were observed for several components, particularly fiber fractions (p < 0.05). The reduction in fiber was more pronounced when enzymes were combined with yeast. Predicted energy values, including metabolizable and digestible energy, were increased following yeast supplementation (p < 0.05). Fermentation characteristics were mainly affected by yeast. Yeast-treated samples exhibited higher pH and ammonia–nitrogen concentrations, indicating increased nitrogen turnover during fermentation. In contrast, lactic and propionic acid concentrations were higher in treatments without yeast, while yeast inoculation was associated with lower acetic acid and slightly higher butyric acid levels. Microbial analysis indicated interactions between treatments for lactic acid bacteria populations, reflecting competition for available substrates. No coliform bacteria were detected, indicating acceptable hygienic quality. Overall, yeast inoculation modified the chemical composition of corn dust, particularly by increasing crude protein and reducing fiber fractions, while NSP enzymes contributed to fiber degradation, especially when combined with yeast. However, these changes reflect compositional modification rather than confirmed feeding value, and further evaluation under rumen or in vivo conditions is required. Full article
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26 pages, 20901 KB  
Article
Equivalent Refractive Index Modeling and Multidomain Characterization of the Temperature Response of Loss in Fiber-Optic Macro-Bends
by Haihui Shen, Dong Yang, Hu Han and Jianli Liu
Sensors 2026, 26(9), 2688; https://doi.org/10.3390/s26092688 (registering DOI) - 26 Apr 2026
Abstract
In the oil and gas industry, fiber-optic telemetry is hindered by transmission degradation from high-temperature macro-bend loss. In this study, to address the lack of a unified model, we develop a numerical framework incorporating both bending-dominated effects and thermo-optic modulation. We systematically analyze [...] Read more.
In the oil and gas industry, fiber-optic telemetry is hindered by transmission degradation from high-temperature macro-bend loss. In this study, to address the lack of a unified model, we develop a numerical framework incorporating both bending-dominated effects and thermo-optic modulation. We systematically analyze the coupled responses of multimode (MMF) and single-mode (SMF) fibers at 1.55 μm across varying temperatures (303.15~483.15 K) and bending radii (1~12 mm). Power spectral density (PSD) and phase spectra are utilized to characterize the loss response and explore its modulation mechanisms. Our results indicate that the MMF temperature response is relatively smooth, with a peak magnitude of 103. In the frequency domain, increased bending raises the MMF PSD main peak by over an order of magnitude, enhancing structural features. While the MMF phase response exhibits a wide dynamic range under tight bending, it becomes unstable in weak modulation regions. Conversely, SMF exhibits more pronounced structural fluctuations (order of 104) but maintains a continuous, smooth phase gradient, demonstrating superior stability. Furthermore, MMF frequency-domain characteristics are highly wavelength-dependent (1.2~2.0 μm), whereas SMF fluctuations remain below 10%, indicating a higher parameter robustness. These findings provide a theoretical foundation for optimizing downhole fiber-optic telemetry selection and signal processing strategies. Full article
(This article belongs to the Section Optical Sensors)
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22 pages, 6790 KB  
Article
Enhancing the Capacity of Voided Reinforced GFRP One-Way Slabs Using Tact Bundled Waste Plastic Bottles
by Hend S. Zayan, Ziadoon M. Ali, Omar Z. Amer and Akram S. Mahmoud
Eng 2026, 7(5), 196; https://doi.org/10.3390/eng7050196 (registering DOI) - 26 Apr 2026
Abstract
Many researchers are interested in the behavior of voided concrete members, which are used to increase flexural capacity by increasing the section depth. However, few studies have concerned ways to expand the voids to increase the self-weight loss while maintaining the permitted carrying [...] Read more.
Many researchers are interested in the behavior of voided concrete members, which are used to increase flexural capacity by increasing the section depth. However, few studies have concerned ways to expand the voids to increase the self-weight loss while maintaining the permitted carrying capacity of these members. This study deals with the effect of a void scheme to further reduce the weight of glass fiber-reinforced polymer (GFRP) one-way reinforced concrete (RC) slabs by benefiting from the anti-classical curvature of one-way RC slabs. This study also incorporates green structures by using non-degradable plastic bottles in the RC one-way slabs. Three void configurations are used in this study: straight-line, back-to-back, and front-to-front void schemes. Specimens with the straight-line void configuration achieve the highest ultimate load capacity, reaching 91% of the solid reference slab’s capacity if reinforced near the minimum reinforcement ratio and 101% if used with a 30% larger GFRP reinforcement ratio. The back-to-back curved configuration is identified as the most efficient design, providing a 10.3% reduction in self-weight while retaining 83% of the solid slab’s ultimate load capacity, as compared with the front-to-front configuration, which retains only 73%. Full article
(This article belongs to the Section Chemical, Civil and Environmental Engineering)
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22 pages, 5736 KB  
Review
A Comparative Review of Biological, Electrochemical, and Membrane-Based Methods for Direct Ocean Carbon Capture
by Zhe Wang, Jiayu Zheng, Siyuan Guo, Ting Zhang, Zhen Wang, Hang Cao, Gang Kevin Li, Shupeng Li and Yi Yang
Materials 2026, 19(9), 1763; https://doi.org/10.3390/ma19091763 (registering DOI) - 26 Apr 2026
Abstract
Direct ocean carbon capture (DOC) has emerged as a promising strategy for mitigating atmospheric CO2 levels and addressing ocean acidification. Unlike direct air carbon capture methods, DOC leverages the ocean’s vast carbon storage capacity, offering a scalable and efficient route for carbon [...] Read more.
Direct ocean carbon capture (DOC) has emerged as a promising strategy for mitigating atmospheric CO2 levels and addressing ocean acidification. Unlike direct air carbon capture methods, DOC leverages the ocean’s vast carbon storage capacity, offering a scalable and efficient route for carbon dioxide removal. This systematic comparative review categorizes existing DOC methods into three types: (1) biological carbon capture, which relies on photosynthesis by microalgae and marine microorganisms; (2) electrochemical carbon capture, which utilizes water electrolysis to generate H+ and OH ions for pH-driven CO2 removal; and (3) physical carbon capture, which employs hollow fiber membranes to directly separate CO2 from seawater. For each technology, we evaluate efficiency, energy consumption, cost, technology readiness level (TRL), scalability, and major challenges. By integrating recent pilot data and providing a critical assessment, this review offers a roadmap for future research in direct seawater CO2 capture. The comparative analysis reveals that electrochemical methods achieve the highest efficiency (60–85%) but face membrane fouling and electrode degradation challenges, while biological methods offer low-energy operation but suffer from slow kinetics and high harvesting costs, and membrane-based methods provide high removal rates (up to 94%) but require improved fouling resistance. Full article
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16 pages, 4163 KB  
Article
Methods for Improving the Straightness Accuracy of Laser Fiber-Based Collimation Measurement
by Ying Zhang, Peizhi Jia, Qibo Feng, Fajia Zheng, Fei Long, Chenlong Ma and Lili Yang
Sensors 2026, 26(9), 2676; https://doi.org/10.3390/s26092676 (registering DOI) - 25 Apr 2026
Abstract
Laser fiber-based collimation straightness measurement can eliminate the intrinsic drift of the laser source while offering a simple configuration and simultaneous measurement of straightness in two orthogonal directions. As a high-precision optoelectronic sensing method, it has been widely used for the measurement of [...] Read more.
Laser fiber-based collimation straightness measurement can eliminate the intrinsic drift of the laser source while offering a simple configuration and simultaneous measurement of straightness in two orthogonal directions. As a high-precision optoelectronic sensing method, it has been widely used for the measurement of straightness, parallelism, perpendicularity, and multi-degree-of-freedom geometric errors. However, two common issues remain in practical applications. One is the nonlinear response of the four-quadrant detector, the core position-sensitive sensor, which is caused by detector nonuniformity and the quasi-Gaussian distribution of the spot. The other is the degradation of measurement performance by atmospheric inhomogeneity and air turbulence along the optical path, particularly in long-distance measurements. To address these issues, a two-dimensional planar calibration method is first proposed to replace conventional one-dimensional linear calibration. A polynomial surface-fitting model is introduced to correct the nonlinear response and inter-axis coupling errors of the four-quadrant photoelectric sensor. Simulation and experimental results show that the proposed method significantly reduces the standard deviation of calibration residuals and improves measurement accuracy. In addition, based on our previously developed common-path beam-drift digital compensation method, comparative experiments were carried out on double-pass common-path and single-pass optical configurations employing corner-cube retroreflectors, and theoretical simulations were performed to analyze the influence of air-turbulence disturbances on measurement stability. Both theoretical and experimental results show that the double-pass common-path configuration exhibits more pronounced temporal drift. Therefore, a real-time digital compensation method for beam drift in long-distance single-pass common-path measurements is proposed. Experimental results demonstrate that the proposed method effectively suppresses drift induced by environmental air turbulence and thereby improving the accuracy and stability of long-travel geometric-error and related straightness measurement for machine-tool linear axes. Full article
(This article belongs to the Special Issue Intelligent Sensors and Signal Processing in Industry—2nd Edition)
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29 pages, 7625 KB  
Article
The Effect of the Extraction Medium (A Natural Deep Eutectic Solvent-Derived System vs. Ethanol) on the Properties of Electrospun PVA Fibers Containing Quercus robur Extracts
by Julia Wnękowicz, Daniel Szopa, Paulina Wróbel, Julia Zwolińska, Maciej Kaniewski, Jacek Chęcmanowski and Anna Witek-Krowiak
Materials 2026, 19(9), 1730; https://doi.org/10.3390/ma19091730 - 24 Apr 2026
Viewed by 98
Abstract
This study examined how the extraction medium used to obtain Quercus robur extracts influenced the properties of electrospun poly(vinyl alcohol) (PVA) mats intended for potential active packaging applications. Extracts prepared with 50% ethanol and with a choline chloride:lactic acid:water system were incorporated into [...] Read more.
This study examined how the extraction medium used to obtain Quercus robur extracts influenced the properties of electrospun poly(vinyl alcohol) (PVA) mats intended for potential active packaging applications. Extracts prepared with 50% ethanol and with a choline chloride:lactic acid:water system were incorporated into PVA spinning solutions, and their effects on solution properties, fiber morphology, thermal behavior, crosslinking response, and polyphenol release were evaluated. The type of extraction medium affected both the electrospinning process and the structure of the resulting materials. Ethanol-derived extracts reduced solution viscosity and promoted the formation of thinner fibers, whereas systems containing the choline chloride:lactic acid:water-derived extract showed higher conductivity and lower electrospinning stability. Crosslinking with tannic acid in water led to the collapse of the fibrous structure, while ethanolic tannic acid treatment preserved the nanofibrous morphology more effectively. FTIR analysis indicated differences in intermolecular interactions within the polymer matrix, consistent with the observed changes in structural stability and release behavior. Thermal analysis showed that ethanol-derived extracts lowered the thermal stability of the PVA matrix, whereas the choline chloride:lactic acid:water-derived system altered the degradation pathway and increased the amount of solid residue formed during heating. Release studies demonstrated a rapid burst release for ethanol-based mats and a more sustained release profile for mats containing the choline chloride:lactic acid:water-derived extract. Selected extract-containing and ethanol–tannic acid-crosslinked mats also showed antibacterial activity against Staphylococcus aureus. The results showed that the extraction medium significantly affected polymer–extract interactions and the functional properties of electrospun PVA mats. At the same time, the conclusions refer specifically to the tested solvent systems, and broader generalization to other natural deep eutectic solvent-type formulations requires further comparative studies. Full article
22 pages, 8493 KB  
Article
Effect of Microscopic Pore Structure on the Mechanical Properties of Raw Phosphogypsum–Basalt Fiber Cementitious Materials
by Guihong Xu, Li He, Yan Zhang, Weihong Xu, Ziwei Chen, Qianbao Fang, Yonghui Li and Weibiao Zhang
Buildings 2026, 16(9), 1654; https://doi.org/10.3390/buildings16091654 - 23 Apr 2026
Viewed by 184
Abstract
This study investigates the mechanical properties and internal pore structure characteristics of raw phosphogypsum–basalt fiber (RPG-BF) cementitious materials with varying raw phosphogypsum (PG) replacement ratios. Specifically, six different PG addition levels (0%, 3%, 6%, 9%, 12%, and 15% by mass of cementitious materials) [...] Read more.
This study investigates the mechanical properties and internal pore structure characteristics of raw phosphogypsum–basalt fiber (RPG-BF) cementitious materials with varying raw phosphogypsum (PG) replacement ratios. Specifically, six different PG addition levels (0%, 3%, 6%, 9%, 12%, and 15% by mass of cementitious materials) with a constant basalt fiber dosage of 0.1% (by volume of concrete) were adopted. The mechanical properties of RPG-BF cementitious materials were evaluated by testing the 7-day and 28-day compressive strengths, 28-day split tensile strength, and 28-day flexural strength. Meanwhile, the pore distribution characteristics of the RPG-BF cementitious materials were systematically analyzed using liquid nitrogen adsorption (LNA) tests and scanning electron microscopy (SEM) observations. The experimental results indicate the following: (a) With an increase in PG content, the mechanical properties of RPG-BF cementitious materials exhibit a significant downward trend: the 28-day compressive strength, split tensile strength, and flexural strength decrease by 49%, 44%, and 43%, respectively. (b) The internal pores of the RPG-BF cementitious materials possess excellent fractal characteristics, with fractal dimensions ranging from 2.52 to 2.62. As the PG content increases, the pore structure becomes more intricate and less homogeneous, which is a microstructural factor associated with the degradation of mechanical properties. (c) There exists a strong Pearson’s linear correlation (R > 0.82, with R2 ranging from 0.67 to 0.94) between the pore fractal dimension of RPG-BF cementitious materials and their 7-day/28-day compressive strength, split tensile strength, and flexural strength. (d) SEM observations show that the quantity of micropores and microcracks in the RPG-BF cementitious materials increases with increasing PG content, further confirming deterioration of the material microstructure. Full article
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18 pages, 8664 KB  
Article
Metagenomic Profiling Reveals Extensive Bacterial Diversity in Chicken Manure and Associated Contaminated Wastewater
by Sadir Zaman, Nawab Ali, Waheed Ullah, Nadia Taimur, Noor ul Akbar, Aiman Waheed, Niaz Muhammad and Muhammad Saeed Khan
Int. J. Mol. Sci. 2026, 27(9), 3741; https://doi.org/10.3390/ijms27093741 - 23 Apr 2026
Viewed by 131
Abstract
Chicken manure and its potential to contaminate water systems through the dispersal of pathogenic bacteria are major concerns in environmental and public health. In this study, a metagenomic analysis was employed to systematically identify and compare bacterial assemblages in chicken manure (CM) and [...] Read more.
Chicken manure and its potential to contaminate water systems through the dispersal of pathogenic bacteria are major concerns in environmental and public health. In this study, a metagenomic analysis was employed to systematically identify and compare bacterial assemblages in chicken manure (CM) and in a contaminated sample of chicken manure wastewater (CMW). Whole DNA was extracted from CM and CMW, followed by whole-genome shotgun sequencing; data analysis was done using online Galaxy software (ver. 26.0.1.dev1). Metagenomic analysis reveals a complex One Health challenge. Data showed that CM and CMW are different in their microbiota, as indicated by a distinct separation of beta diversity values and limited overlapping of species between sample types. In the current study, we found a greatly significant common functional set of adapted bacterial masses, including major pathogenic bacterial groups as well as opportunistic and environmental bacterial species, indicative of a direct contamination from CM and CMW. Notably, in both CM and CMW, a plethora of opportunistic, enteric, and environmental pathogens like Escherichia coli, Salmonella enterica, and Acinetobacter baumannii were found, coupled with an indication of a direct functional flow between both ecosystems as tangled reservoirs. Chicken manure samples showed differences in taxonomic composition and inferred functional profiles at the time of sampling: CM1 was pathogen-enriched, CM2 exhibited strong nitrogen-supportive metabolism, CM3 was dominated by fiber-degrading decomposers, and CM4 showed high methane-producing potential with environmental risk. Such findings underscore the raising of chickens as a potential source of harmful bacteria for the environment. It is important to note that this study represents a preliminary investigation with certain limitations, including the absence of biological replicates, lack of temporal sampling, and limited capacity to infer dynamic ecological interactions. Yet this metagenomic report is more about describing the taxonomy and functional potential of the bacteria, rather than discussing the actual ecological processes of these microorganisms in the environment. Future studies will be required to explore these aspects. Full article
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26 pages, 3412 KB  
Article
Experimental Investigation on the Effect of Wetting–Drying Cycles on Bond Performance of GFRP Adhesive Anchors in Concrete
by Yifan Xu, Wensheng Liang, Xianghong Ding and Yanjie Wang
Buildings 2026, 16(9), 1649; https://doi.org/10.3390/buildings16091649 - 22 Apr 2026
Viewed by 161
Abstract
The long-term durability of adhesive anchors in aggressive environments is a critical concern for infrastructure safety, with steel corrosion being one of the most detrimental phenomena. While glass fiber-reinforced polymer (GFRP) anchors offer corrosion-resistant alternatives to steel anchors in harsh marine environments, the [...] Read more.
The long-term durability of adhesive anchors in aggressive environments is a critical concern for infrastructure safety, with steel corrosion being one of the most detrimental phenomena. While glass fiber-reinforced polymer (GFRP) anchors offer corrosion-resistant alternatives to steel anchors in harsh marine environments, the bond performance at the anchorage interface progressively deteriorates under wetting–drying (WD) cycles, which may compromise long-term anchorage integrity. However, the bond characteristics of GFRP anchors under WD exposure, particularly the development of predictive models, remain insufficiently understood. This paper presents an experimental investigation into the impact of WD cycles on the bond of GFRP adhesive anchors in concrete. Twenty-four specimens were tested under pull-out loads, considering two key variables: bonded length (40 mm and 80 mm, corresponding to 5 and 10 times the bar diameter) and number of WD cycles (0, 30, 60, and 90). Artificial seawater was prepared via ASTM D1141-98 to simulate marine exposure conditions. The results revealed that both bond strength and bond stiffness decreased significantly with increasing WD cycles, while the failure mode progressively shifted from the bar–adhesive interface to the adhesive–concrete interface. Based on the experimental data, a cycle-dependent bond strength model was developed to predict the bond degradation of the anchor–concrete interface after WD exposure. Requiring only the undegraded concrete strength, the proposed model effectively captures the coupled effects of WD cycles and bonded length on bond strength degradation, presenting a practical tool for the durability design and service life evaluation of GFRP anchorage systems in coastal and marine environments. Full article
19 pages, 2392 KB  
Article
Synergistic Modification of Steam Explosion Combined with Enzymatic Hydrolysis on Wheat Bran to Improve Dough Properties, Bread Quality, and In Vitro Digestibility
by Xiaoxuan Li, Xiaomeng Guo, Jie Yu, Zixin Zhao, Xue Tian, Wenjie Sui, Jing Meng, Tao Wu and Min Zhang
Foods 2026, 15(9), 1465; https://doi.org/10.3390/foods15091465 - 22 Apr 2026
Viewed by 218
Abstract
Wheat bran, as a major nutrient-rich agricultural by-product, is underutilized due to poor functional properties. This study investigated the synergistic effects of steam explosion (SE), enzymatic hydrolysis (EH), and SE combined with EH (SE-EH) on wheat bran to improve the dough properties, bread [...] Read more.
Wheat bran, as a major nutrient-rich agricultural by-product, is underutilized due to poor functional properties. This study investigated the synergistic effects of steam explosion (SE), enzymatic hydrolysis (EH), and SE combined with EH (SE-EH) on wheat bran to improve the dough properties, bread quality, and in vitro starch digestion. Results showed that SE destroyed the dense structure of wheat bran to form a porous surface morphology and promoted the conversion of insoluble dietary fiber (IDF) to soluble dietary fiber (SDF). This structural loosening facilitated further fiber degradation for subsequent EH and achieved the obvious improvements in hydration properties after combined treatment. For the dough system, the addition of SE-EH bran increased the water absorption, hardness, and viscosity, but reduced the development and stability time of the dough, in comparison with the control dough. These changes suggested that the modified bran altered dough hydration behavior and gluten network continuity, contributing to the increment of bread’s specific volume. The starch hydrolysis rate of bread adding SE-EH wheat bran was decreased; the slowly digestible starch (SDS) and resistant starch (RS) contents were 2.59-fold and 1.31-fold higher than the control group, respectively. Additionally, the incorporation of modified wheat bran delayed bread hardening during storage, with the SE-modified group showing the best effect. Wheat bran modification enhanced its processing functionality, providing a feasible approach for bread production to improve storage stability and nutritional quality. Full article
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13 pages, 1228 KB  
Article
Multi-Target Restoration of Dermal Elastic Fibers Through Elastin Upregulation, Elastase Suppression, and Scaffold Reinforcement
by Sanghyun Ye, Seongsu Kang, Eui Taek Jeong, Seung-Hyun Jun and Nae-Gyu Kang
Curr. Issues Mol. Biol. 2026, 48(5), 431; https://doi.org/10.3390/cimb48050431 - 22 Apr 2026
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Abstract
Elastic fibers are key components of the skin extracellular matrix and are essential for maintaining skin integrity and elasticity. During skin aging, particularly photoaging, elastic fiber integrity is progressively compromised by increased elastase activity and the downregulation of elastin and scaffold-related gene expression. [...] Read more.
Elastic fibers are key components of the skin extracellular matrix and are essential for maintaining skin integrity and elasticity. During skin aging, particularly photoaging, elastic fiber integrity is progressively compromised by increased elastase activity and the downregulation of elastin and scaffold-related gene expression. Therefore, effective strategies to preserve elastic fiber function should address not only elastin synthesis but also enzymatic degradation and scaffold integrity. In this study, we investigated a multitarget approach to restoring the elastic fiber network by modulating elastin production, elastase activity, and scaffold protein expression. We found that Copper Tripeptide-1 enhanced elastin expression and secretion, ethyl ferulate inhibited elastase activity, and cedrol promoted scaffold-related gene expression and microfibrillar protein restoration in dermal fibroblasts. To assess the biological relevance of this approach, the combined treatment was evaluated using UV-damaged human skin biopsy samples. This combination effectively mitigated UV-induced elastic fiber disruption and significantly improved fiber architecture, as confirmed by immunofluorescence staining and scanning electron microscopy. These findings indicate that coordinated modulation of elastin production, proteolytic protection, and scaffold reinforcement is essential for maintaining elastic fiber integrity and represents a promising approach for preserving skin elasticity during aging. Full article
(This article belongs to the Special Issue Exploring Molecular Pathways in Skin Health and Diseases)
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