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New Insight into Bacteriophage and Their Potential Applications

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 20 August 2026 | Viewed by 808

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Institute for Digital Molecular Analytics and Science (IDMxS), Nanyang Technological University (NTU), 59 Nanyang Drive, Experimental Medicine Building, Level 6 & 7, Singapore 636921, Singapore
Interests: phage; antibacterial drug; mycobacterial
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Special Issue Information

Dear Colleagues,

The field of bacteriophage research is undergoing a rapid transformation, driven by an ever-deepening understanding of phage biology and its translational potential. As the smallest yet most abundant biological entities, bacteriophages play a pivotal role in microbial ecosystems and serve as powerful tools for elucidating bacterial processes. Advances in genomics, structural biology, and synthetic biology have unveiled new dimensions of phage–host interactions, fostering innovative applications that span medicine, agriculture, and biotechnology.

In response to this exciting progress, we are delighted to introduce this Special Issue, entitled New Insight into Bacteriophage and Their Potential Applications”. This Special Issue welcomes contributions that explore groundbreaking discoveries in phage biology, uncover novel mechanisms of action, and highlight emerging applications of bacteriophages. By showcasing interdisciplinary research, this Special Issue aims to bridge the gap between fundamental science and practical innovations, paving the way for transformative solutions to contemporary challenges such as antimicrobial resistance, environmental sustainability, and precision medicine.

Dr. Wilfried Moreira
Guest Editor

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Keywords

  • bacteriophage
  • phage biology
  • phage-host interactions
  • antimicrobial resistance

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Published Papers (1 paper)

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Research

17 pages, 3790 KB  
Article
Characterization of the Fiber Protein C-Terminal Domain from Klebsiella pneumoniae Phage KlebP_144 and Evaluation of Its Anti-Capsular Activity
by Bogdana I. Kravchuk, Natalia N. Golosova, Ekaterina A. Kondakova, Yana A. Khlusevich, Vyacheslav I. Yakubovskij, Margarita I. Arisova, Yuliya N. Kozlova, Nina V. Tikunova and Andrey L. Matveev
Int. J. Mol. Sci. 2026, 27(9), 3883; https://doi.org/10.3390/ijms27093883 - 27 Apr 2026
Viewed by 246
Abstract
Klebsiella pneumoniae, a member of the ESKAPEE group of priority pathogens, has become one of the most challenging bacterial pathogens in modern clinical practice, largely due to its multidrug resistance and the immune-evasive effect of its capsular polysaccharide (CPS). Phage-encoded depolymerases, which [...] Read more.
Klebsiella pneumoniae, a member of the ESKAPEE group of priority pathogens, has become one of the most challenging bacterial pathogens in modern clinical practice, largely due to its multidrug resistance and the immune-evasive effect of its capsular polysaccharide (CPS). Phage-encoded depolymerases, which selectively degrade the capsular polysaccharide, have emerged as promising antimicrobial agents capable of restoring bacterial susceptibility to both immune clearance and phage infection. The fragment corresponding to the C-terminal region of a putative depolymerase of bacteriophage KlebP_144, namely DepKP144ΔC, was cloned, expressed in E. coli, and purified using immobilized metal affinity chromatography. DepKP144ΔC displays an enzymatic activity against capsular polysaccharides of 100% K1 capsular-type strains and 85% K2 capsular-type strains, including classical and hypervirulent isolates. It was demonstrated that this protein is capable of inhibiting K. pneumoniae biofilm formation, but it is unable to disrupt mature biofilms. In vivo experiments using a murine K. pneumoniae infection model further confirmed its therapeutic potential: treatment with DepKP144ΔC improved survival rate in mice infected with K2-type K. pneumoniae, indicating significant attenuation of bacterial virulence. Therefore, these results demonstrate the potential role of the C-terminal domain of the bacteriophage KP144 tail-fiber protein in phage entry and show that its carbohydrate-recognition motifs possess enzymatic activity against the Klebsiella capsular polysaccharides. Full article
(This article belongs to the Special Issue New Insight into Bacteriophage and Their Potential Applications)
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