Search Results (836)

Honey bees are constantly exposed to various environmental threats, among which pesticide pollution, including fungicides, is one of the most serious. The bees were 3 days old when they received the experimental solution. This study aimed to evaluate the behavior and mortality of honey bee workers exposed to a commercial formulation of the fungicide tebuconazole (Tebu^® EW, a.i. 25.8%; HELM, Hamburg, Germany). The experiment was conducted under laboratory conditions and lasted 7 days for all experimental groups. The fungicide solution was prepared by adding 6.25 mL of Tebu^® EW per 1 L of water, corresponding to 156.25 mg of tebuconazole (active ingredient) in the prepared solution of sugar syrup. The solution was served in 5 mL dispensers (=group feeding) placed in the cages. This concentration was used for the acute-exposure group (24 h). After 24 h bees were supplied with untreated sugar syrup for the remainder of the experiment. For the chronic-exposure group (168 h), the solution was a 1000-fold dilution of the acute solution, containing 0.15625 mg tebuconazole, dissolved in sugar syrup, provided continuously for 7 days with daily replacement. After 7 days, bee behavior was recorded using a camera and analyzed with Noldus Observer XT software (12.5: Windows 7 64-bit (SP1) version) Five basic honey bee behaviors were examined: walking, flight, self-grooming, contact between individuals and stillness. The results showed statistically significant differences between the experimental groups and the control group (α = 0.05) in the duration of walking, contact between individuals and self-grooming, and the frequency of walking and flight. This was particularly evident for self-grooming; the longer the group was exposed to tebuconazole, the less time the bees spent on this behavior (the acute group spent 47% less time self-grooming and the chronic group spent 88.8% less time self-grooming compared to the control group). Meanwhile, the frequency of walking and flying increased significantly with increasing exposure. No significant differences were observed in the survival between the groups. Based on these findings, it can be concluded that the fungicide containing tebuconazole significantly affects the behavior of honey bee workers. Full article

Yaks (Bos grunniens) on the Qinghai–Tibetan Plateau face severe nutritional limitations during the dry season due to dependence on highly lignified, low-quality roughage. Identifying safe and effective rumen regulators capable of enhancing fiber utilization in this species is therefore of great practical importance. This study employed a two-pronged approach integrating in vitro mechanistic investigation and in vivo validation to evaluate the effects of the amphoteric surfactant cocamidopropyl betaine (CAPB) on rumen fermentation, the micro-spatial distribution of digestive enzymes, apparent total tract digestibility, and the macroscopic growth performance of yaks. In the in vitro fermentation trial (Experiment 1), a randomized block design was employed where a straw-based high-forage diet was used as the substrate and supplemented with 0, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0% CAPB (based on substrate dry matter, DM) for a 48 h batch culture. The results showed that as the CAPB supplementation level increased, cumulative gas production, the degradation rates of DM and neutral detergent fiber (NDF), and the yields of total volatile fatty acids and microbial protein all exhibited significant quadratic responses (p < 0.05), peaking at the 0.5–1.0% supplementation levels. Concurrently, CAPB significantly promoted the transfer and release of carboxymethyl cellulase and xylanase into the free liquid phase (p < 0.01). In the in vivo validation trial (Experiment 2), 24 healthy growing male yaks (initial body weight 131.2 ± 8.4 kg) were allocated in a completely randomized design to four groups and fed a basal diet supplemented with 0, 0.5, 1.0, or 2.0% CAPB for 44 days. The results indicated that, while maintaining a stable DM intake, the addition of 0.5% CAPB significantly increased the average daily gain (ADG) of yaks (p < 0.05), improved the feed-to-gain ratio, and significantly enhanced the apparent total tract digestibility of NDF and ether extract (p < 0.05). However, when the supplementation dose exceeded the safety threshold (≥2.5% in vitro and ≥2.0% in vivo), both fermentation parameters and growth advantages declined. In conclusion, under the present experimental conditions, 0.5% CAPB improved roughage fermentation efficiency, putatively through an ‘enzyme elution’ mechanism, and was associated with macroscopic improvements in NDF and EE apparent digestibility and ADG in growing yaks. These findings identify 0.5% CAPB as a promising candidate rumen regulator for improving roughage utilization in growing yaks; broader generalization will require larger-scale and longer-duration trials. Full article

Monolepta hieroglyphica Motschulsky (Coleoptera: Chrysomelidae) (M. hieroglyphica) is widely distributed in China. Its larvae are soil pests that cause severe damage to the seeds and roots of economically important crops such as corn, cotton, and millet. This study investigated the effects of four temperatures (25, 28, 31, and 34 °C) on the survival rate, food consumption (3rd instar), pupation rate, emergence rate, biometric indices (weight and length), and antioxidant enzyme activity of immature M. hieroglyphica. High temperatures (31 °C and 34 °C) adversely affected developmental duration, survival rates, and feeding efficiency. The highest pupation rate, emergence rate, and biometric indices were observed at 28 °C, after which these metrics steadily declined as the temperature increased. Notably, emergence was completely inhibited at 34 °C, resulting in the absence of biometric data. These changes correspond with the temperature-dependent regulation of antioxidant enzyme activities (SOD, CAT, GST, and POD). This study identified the optimal temperature range and critical high-temperature threshold for immature M. hieroglyphica, providing key biological parameters for predicting population dynamics and outbreak risks under climate warming, and offering a scientific basis for precise monitoring and temperature-based integrated pest management strategies. Full article

Long-duration animal trajectories are central to computational ethology, yet constructing large rodent cohorts remains costly, time-intensive, and constrained by animal-use considerations. We present a sensor-aware generative framework that separates latent behavioral dynamics from sensing-induced observation distortion to synthesize observed-domain trajectories that are behaviorally plausible while reproducing proxy-referenced observation distortions. The framework combines a run-level semi-Markov ethology model, occupancy calibration, and state-conditioned kinematic generation with a regime-dependent Ultra-Wideband observation channel that explicitly captures Line-of-Sight and Non-Line-of-Sight sensing conditions. Using four UWB sessions, this proof-of-concept study models three states—exploring, feeding, and burrowing—and evaluates realism through state occupancy, state-conditioned kinematic divergence, residual-domain agreement, and mean-squared displacement across time lags. We further assess whether sensor-aware conditioning improves robustness under LoS/NLoS domain shift in downstream trajectory classification. Sensor-aware conditioning yields stable mixed-domain performance with AUC = 0.995, whereas condition-agnostic baselines decline to AUC = 0.974 and AUC = 0.901. These results support the feasibility of sensor-aware in silico ethology as a proof-of-concept framework for controlled robustness studies and algorithm evaluation under proxy-referenced observation distortion. Because the present evaluation is based on four UWB sessions and uses a smoothed UWB-derived reference trajectory rather than independent ground truth, broader applications to synthetic-cohort generation, disease modeling, and statistical power-analysis workflows should be considered future directions requiring validation in larger datasets. Full article

The present work examines a severe, long-lived bow echo in South China during 12–13 April 2016 and investigates the favorable factors for its strength and longevity using a series of 20 cloud-resolving ensemble experiments. Analysis of observational data indicated that this system developed near a surface front under unstable and favorable conditions with dynamic uplifting by approaching troughs at 500–700 hPa. After formation, it propagated rapidly toward the east–southeast across South China and made landfall in Southern Taiwan. The ensemble used four different datasets as initial and boundary conditions and started at five different initial times, whereby comparing the better-performing members with worse ones, four key factors promoting its strength and longevity were identified: (1) A stronger and moister low-level southwesterly flow to the south of the front to enhance convergence and moisture flux at the leading edge—where a stronger inflow with higher equivalent potential temperature (θ_e) values could feed into the bow echo—leading to a stronger and taller updraft and overall more abundant hydrometeors and rainfall; (2) stronger northwesterly to westerly winds near 700 hPa and thus stronger low-level vertical wind shear, resulting in a stronger rear inflow jet (RIJ), bookend vortices behind the bow apex, and, eventually, a faster propagation speed; (3) a deeper low to the northeast of the bow echo near 850 hPa, where its circulation also helped to bring in low-θ_e air from farther away and enhance the RIJ and cold pool; and (4) a convective initiation location farther to the east in a more favorable environment, with higher θ_e and a faster speed to remain in such a better environment. Helped by the above factors, the bow echo in the present case could reach the observed severity and long duration (~15 h) through interactions and reinforcement among its structural components, including the tilted updraft/downdraft, the low-level inflow and stratiform region, the RIJ and bookend vortices, and the cold pool and gust front. Full article

To address the poor coating quality and low efficiency of Astragalus membranaceus seed pelletizing, this study combined theoretical analysis, DEM simulations, and experiments. The motion and force conditions of seed-powder particles were analyzed to identify key parameters. Using the coefficient of variation (Cv) as the evaluation index, the disc diameter, pan edge inclination, and rotational speed were optimized via response surface methodology. The optimal structural parameters were 605.5 mm, 15.7°, and 20.3 r·s⁻¹. Liquid adhesion was represented by a custom time-varying cohesion model in DEM. Physical experiments showed that the optimized structure increased the pelletization qualification rate from 74.8% to 94.3%. Orthogonal experiments further optimized the process parameters: a single powder feed of 20 g, a single binder solution feed of 25 mL, and a coating duration of 8 min, achieving a qualification rate of 98.3%. Seedling emergence tests revealed that pelleted seeds had a significantly higher emergence rate (97.6%) than non-pelleted seeds (67.3%). These findings provide theoretical and technical references for pelletizing the coating of irregularly shaped seeds. Full article

Apolygus lucorum (Meyer-Dür) is a phytozoophagous crop pest. While the effects of plant-based diets on its development and reproduction have been extensively studied, the combined effects of plant- and prey-based diets on these traits remain poorly understood. This study systematically evaluated the effects of plant-only, prey-only, and mixed plant–prey diets on A. lucorum nymphal survival and development, as well as adult longevity and fecundity, under controlled laboratory conditions. The results demonstrate that diet composition significantly affected nymphal survival and developmental progression. Nymphs fed exclusively on prey (Aphis gossypii Glover or Bemisia tabaci (Gennadius) nymphs) failed to complete juvenile development. Although a diet of Helicoverpa armigera (Hübner) eggs alone enabled some individuals to reach adulthood, survival rates were significantly lower than those in mixed-diet treatments. Mixed feeding markedly improved nymphal survival, with the highest rates observed in groups fed green beans + H. armigera eggs and cotton leaves + B. tabaci nymph combinations (both 64.45%). The developmental duration was also influenced. Mixed diets, particularly green beans + H. armigera eggs, significantly shortened each instar and the total developmental time (11.04 ± 0.17 d), whereas a diet of cotton leaves alone prolonged development (19.45 ± 0.24 d). Adult longevity and reproductive output were likewise diet-dependent. The longest lifespans were recorded in adults fed green beans alone or green beans + H. armigera eggs, while the shortest lifespan was observed for those fed only cotton leaves. Successful oviposition was only achieved following four dietary treatments: green beans alone, green beans + H. armigera eggs, H. armigera eggs alone, and cotton leaves + H. armigera eggs. Among these, the green bean + H. armigera egg diet yielded the best reproductive performance, featuring the shortest pre-oviposition period (5.82 ± 0.60 d), the longest oviposition period (19.41 ± 1.68 d), and the highest mean fecundity per female (238.35 ± 25.51 eggs). This underscores the reproductive advantage of a mixed plant–prey diet. This study clarifies how dietary conditions shape the survival, development, and reproduction of A. lucorum, highlighting its strong reliance on nutritional quality for key life-history traits. These findings offer valuable insights into the ecological adaptations underlying the feeding behavior of this insect. Full article

Background: School feeding programs aim to improve child nutrition, and they may influence weight outcomes insofar as program modalities and household responses alter children’s total energy intake. This is especially relevant in countries facing the double burden of malnutrition, where undernutrition and micronutrient deficiencies coexist with rising overweight and obesity. This study estimates the effect of Peru’s former National School Feeding Program on obesity and excess weight among children aged 36 to 59 months under a selection-on-observables identification strategy and assesses whether impacts differ across operational modalities, particularly breakfast-only versus breakfast plus lunch and ready-to-eat rations versus foods delivered for preparation. Methods: We use repeated cross-sectional microdata from the Demographic and Health Survey (ENDES) pooled over 2014 to 2018 and link them to administrative information. The sample includes 18,959 children aged 36 to 59 months. To improve comparability, we estimate propensity score weights targeting the average treatment effect on the treated (ATT) using a machine learning generalized boosted model (GBM), and assess covariate balance using standardized mean differences and Kolmogorov–Smirnov statistics. Identification assumes conditional independence given observed covariates and overlap (common support). Main estimates rely on weighted probit models with fixed effects, progressively adding exposure duration, modality indicators, and controls. Distributional effects are examined using quantile regression on the continuous weight-for-height z-score. Results: Without differentiating modalities, beneficiary status is not associated with a statistically significant change in obesity, while pooled baseline estimates indicate a statistically significant higher probability of excess weight. Modality-specific results show that obesity declines only when Qali Warma is delivered as breakfast plus lunch through products to be prepared (approximately −1.0 percentage point in parsimonious models and −0.4 percentage points after controls). Evidence for excess weight is directionally consistent by modality but less conclusive once controls are included. Conclusions: Qali Warma’s effects on early-childhood weight outcomes depend on implementation modality. Evaluations of school feeding programs should incorporate operational heterogeneity, particularly during program redesign. Full article

This experiment aimed to evaluate physiological and behavioral responses of crossbred Dehong dairy buffaloes to heat stress (HS) in comparison with those in a thermoneutral (TN) environment. Twelve crossbred dairy buffaloes at similar lactation stages were randomly allocated to two groups of six animals each. Six buffaloes were exposed to HS conditions and the other six to TN conditions in an open loose-housing barn without individual stalls. Respiration rates were manually recorded at 08:00 h, 13:00 h, and 18:00 h. Duration and frequency of behaviors (standing, lying, feeding, and drinking) were continuously monitored using digital cameras for 20 consecutive days. Compared with the TN group, HS-exposed buffaloes exhibited markedly higher respiration rates (p < 0.001) and feeding frequencies (p < 0.05), but significantly shorter feeding duration throughout the observation period (p < 0.05). No significant differences were observed in the time spent standing, lying, or drinking between the two groups (p ≥ 0.05). Under HS conditions, buffaloes preferred a vertical lying posture to reduce exposure to intense solar radiation. These results suggest that crossbred Dehong dairy buffaloes can adapt to heat stress by modulating their physiological and behavioral strategies. The observed changes in physiological indices and behavioral patterns provide fundamental data for further elucidating the heat stress adaptation mechanisms in dairy buffaloes. Full article

Inefficient urban traffic management remains a critical challenge, as conventional signal controllers—built on fixed timing plans—cannot cope with the dynamic nature of modern city traffic. This study addresses this limitation by developing a decentralized MARL-based framework capable of coordinating five interconnected intersections as a unified traffic cell. Central to the proposed solution is the Strategic Anti-Blocking Phase Adjustment (SAPA) module, which enables intersections to autonomously modify phase durations in response to real-time traffic conditions. The framework is designed to handle heterogeneous demand patterns, with particular emphasis on arterial corridors connecting urban centers to peripheral zones. Integration of a Visible Light Communication (VLC) network allows continuous monitoring of key variables, including vehicle kinematics and pedestrian activity, feeding the agents with rich environmental feedback. Experimental evaluation confirms the effectiveness of the approach: the SAPA-augmented DQN achieves roughly 33% shorter vehicle queues and a ~70% reduction in pedestrian waiting counts relative to a standard DQN baseline. Remarkably, these gains bring the value-based method to a performance level comparable to MAPPO, a considerably more complex multi-agent policy optimization algorithm, establishing SAPA as an efficient and scalable enhancement for intelligent urban traffic control. Full article

Background/Objectives: Nutrition is essential to outcomes in critically ill children; however, optimal timing, route, and composition of feeding remain uncertain. Prior studies demonstrate considerable variability in study design, patient populations, and outcome measures, limiting comparability. This review synthesizes international pediatric intensive care unit (PICU) nutrition studies evaluating timing, route, and content of nutritional interventions and summarizes associated clinical outcomes and nutritional adequacy. Methods: A comprehensive scoping review was conducted using the PICOS framework. PubMed and Embase databases were searched for studies published between 2015 and 2025 enrolling critically ill children ≤21 years old admitted to PICUs. Eligible studies assessed timing (early vs. late enteral nutrition), nutritional composition, or feeding route (enteral vs. parenteral). Screening and full-text review were performed independently by two reviewers using Covidence, with discrepancies resolved by a third reviewer. Quality assessment used STROBE. The protocol was registered with PROSPERO. Results: Of 652 identified records, 30 studies met inclusion criteria. Studies were conducted primarily in the United States (27%), with additional contributions from Spain and Brazil (10% each) and several other countries. Study designs included randomized controlled trials (27%) and observational studies (73%). Interventions examined feeding route (14%), nutritional content (38%), and timing (48%). Frequently reported outcomes included feeding intolerance or adverse events, duration of mechanical ventilation, time to nutrition goals, PICU length of stay, mortality, and nutritional adequacy. Conclusions: The contemporary PICU nutrition literature demonstrates persistent heterogeneity in practice and outcomes. This review identifies ongoing gaps in timing, delivery, and adequacy of nutritional support. Full article

Background/Objectives: Appetite-regulating hormones are bioactive components of human milk. We tested the associations of leptin and adiponectin with infant growth and eating behaviors to age 6 months. Methods: In a cohort of 70 healthy, full-term infants and their mothers, human milk adiponectin and leptin were assayed at age 2 months (m). At infant ages 2, 4, and 6 m, infant anthropometry was obtained, mothers reported feeding frequency, duration, and breastfeeding intensity and completed the Baby Eating Behavior Questionnaire (Enjoyment of Food, Food Responsiveness, and General Appetite), and infant sucking vigor using an artificial nipple (burst duration and sucking frequency) was measured. Mothers reported demographics, gestational diabetes and pre-pregnancy body mass index (BMI), gestational age, and infant birthweight. Multivariate models evaluated predictors of leptin and adiponectin, and associations of leptin and adiponectin with infant growth and eating behaviors. Results: Human milk leptin was predicted by maternal BMI (β = 0.02) and breastfeeding intensity (β = −0.32). Regarding infant growth, infant weight-for-age and weight-for-length z-scores at 6 m were predicted by leptin (β = 0.91 and β = 1.22, respectively) and adiponectin (β = 0.01 and β = 0.01, respectively). Regarding infant eating behaviors, feeding duration at 2 m and feeding frequency at 4 m were predicted by adiponectin (β = 0.03 and β = −0.02, respectively). Conclusions: Human milk leptin and adiponectin may contribute to weight gain in early infancy, but the effect does not appear to be mediated substantially by infant eating behaviors. Further investigation into the metabolic programming of early infant weight gain is warranted. Full article

Using alternative energy sources for animal feed, such as hybrid corn varieties rather than genetically modified ones, is important. Therefore, the objective of this work was to assess the effects of supplementation at the end of gestation with Mexican Puma hybrid corn grain on productive and behavioral parameters in sheep. Twenty Columbia multiparous ewes were used; along with their diet, they were provided 600 g/animal/day of cracked corn during the last 20 days of pregnancy and the first week of lactation. The animals were divided into two groups: one fed commercial cracked corn (n = 11) and the other Mexican Tlaoli Puma hybrid cracked corn (n = 9). The productive parameters evaluated in the mother were: body weight, body condition score (BCS), feed intake, weight change, glucose, and ketone body levels, as well as the estimated quality of milk using Brix refractometer values on days 15 and 30 of lactation. In lambs, their rectal and external temperature was measured 2 h after birth, while their weight was measured 2 h after birth and every week until week 6 postpartum. Behavioral parameters were measured in the first two hours postpartum, including the maternal latency of cleaning the offspring, duration of the first cleaning episode, the lamb’s latencies of standing and nursing, and vocalizations in mother and lamb. Weight, BCS and weight change were not affected by the group but were affected by time; these parameters increased at the end of gestation and decreased significantly after delivery (p < 0.05). Ketone body levels were not affected by group or time (p > 0.05) and remained at low values. Glucose levels were not affected by the group but were affected by time; they increased significantly after birth (p < 0.05). Feed intake was similar in both groups (p > 0.05) and decreased as parturition approached (p < 0.05). The estimated milk quality was not affected by the group, nor by the time (p > 0.05). Mothers in both groups began cleaning their offspring within the first three minutes after giving birth and emitted a similar frequency of vocalizations (p > 0.05). However, mothers in the commercial maize group had a longer cleaning episode than those in the hybrid maize group (p < 0.05). The lambs in both groups stood up within the first half hour of birth, suckled before one hour after birth and emitted a similar number of vocalizations (p > 0.05). Temperatures and lamb weight were similar in both groups (p > 0.05); however, lamb weight increased as they aged (p < 0.05). It is concluded that supplementing sheep at the end of gestation with Puma hybrid Mexican corn grain can yield similar productive and behavioral benefits as supplementing with commercial grain. Full article

Long-term cold exposure reduces livestock welfare and productivity in Inner Mongolia. This study assessed cold stress effects on 60 two-month-old female Dorper × Mongolia lambs allocated to four sheltering conditions (n = 15): indoor pens with enclosed housing (IP), outdoor pens (OP), house with playground pens (OPP), and polytunnel pens (PP). Compared with IP, OP exhibited significantly lower temperature, humidity, CO₂ concentration, NH₃ concentration, and WCI, and significantly higher wind speed and solar radiant heat (p < 0.001). Humidity, CO₂ concentration, and NH₃ concentration in PP was lower than in IP, but higher than in OP (p < 0.001); temperature, wind speed, and WCI did not differ significantly between PP and IP. ADG was significantly lower in OP and OPP than in IP (p < 0.001), whereas PP did not differ from IP. F:G was higher in OP than in IP and PP (p = 0.040). Feeding duration had significant effects on ACTH, leptin, T₃, T₄, TP, urea, TG, NEFA, LDL, and HDL concentrations. Rearing environment significantly affected GLU, ALB, LDH, and TG. Feeding duration × sheltering conditions interaction significantly influenced ACTH, TP, ALB, urea, LDH, TG, LDL, and HDL. OP induced cold stress and dysfunction, while IP and PP produced milder responses. PP raised indoor temperatures substantially, and is thus optimal for winter lamb production. Full article

The purpose of this study was to examine relationships between infant feeding and parenting self-efficacy. Mothers (N = 121) receiving home visiting reported on PSE and infant feeding at two times (e.g., longitudinally). Mothers were exclusively formula feeding (46.7%), exclusively breastfeeding (19.8%) or combining breastfeeding and formula (33.1%). Infant feeding was regressed on parenting self-efficacy and relevant demographics using logistic regression. Mothers with higher parenting self-efficacy were more likely to be exclusively formula feeding or combination feeding at Time 1. Continued breastfeeding was not predicted by self-efficacy but rather by working status and earlier supplementation. Results suggest higher parenting self-efficacy associated with formula feeding suggests social reinforcement or feelings of success around the enactment of or choice in infant feeding method. Lower parenting self-efficacy associated with initial breastfeeding suggests unsuccessful enactment (i.e., breastfeeding challenges) or negative social reinforcement. More research is needed to understand infant feeding norms and practices in relationship to parenting self-efficacy to best promote breastfeeding intervention and support maternal mental health. Practitioners should work to extend exclusive breastfeeding through supportive positive reinforcement, while limiting formula supplementation. The importance of parental leave for longer breastfeeding duration should be considered when establishing leave policies. Full article