Search Results (333)

Allotetraploid fish produced by distant hybridization are valuable germplasm for the mass production of sterile triploids. The allotetraploid crucian–carp hybrid (4nAT, 4n = 200) is derived from the intergeneric cross between a female red crucian carp (Carassius auratus red var., 2n = 100) and a male common carp (Cyprinus carpio L., 2n = 100). However, after 33 successive generations, this lineage faces a critical bottleneck in maintaining male fertility. The present study aimed to develop new biomarkers for testicular development and characterize the associated functional gene expression profile in 4nAT. Following whole-genome resequencing and selection signature analysis of 15 male 4nAT individuals from each of the high-development group (HDG) and low-development group (LDG), ZSWIM7 (Zinc Finger SWIM-Type Containing 7), a gene implicated in reproductive development, was selected as a candidate for further fertility association studies. Seven SNPs were screened in the coding region of ZSWIM7 of 70 4nAT males; among these, SNP3 (c.23T/C) exhibited a significant correlation between genotypes and testicular development: individuals with the CT genotype showed a higher gonadosomatic index (1.17 ± 0.68 vs. 0.65 ± 0.50) and greater counts of mature spermatozoa (2537.67 ± 283.95 vs. 341.56 ± 121.66) compared to those with the TT genotype. Further quantitative PCR and immunofluorescence assays demonstrated that ZSWIM7 was highly expressed in the testis and specifically localized to the nuclei of early meiotic primary spermatocytes. Collectively, these results establish ZSWIM7 as a promising biomarker for 4nAT testicular development, offering a potential molecular tool for maintaining male fertility in allotetraploid fish breeding. Full article

This study investigated single-nucleotide polymorphisms (SNPs) within the CAPN1 316, CAPN1 4751, and CAST 2959 markers using high-resolution melting (HRM) analysis to predict meat tenderness in crossbred beef cattle. Tenderness was assessed using the Warner–Bratzler shear force (WBSF) test, with results expressed in grams (g), representing the force required to shear muscle fibers. Significant differences in phenotypic data were observed among the genotypic groups. The finding showed that polymorphisms at CAPN1 316, CAPN1 4751, and CAST 2959 exert interactive effects on meat quality traits. Notably, the TT genotype at CAPN1 4751 increased the adjusted WBSF (aWBSF) by approximately 792 g, indicating that TT was an unfavorable variant for tenderness. These results support the use of marker-assisted selection strategies in which the TT genotype is managed to minimize its frequency while other relevant markers are concurrently monitored, thereby enhancing genetic progress in meat tenderness across commercial cattle populations. This study demonstrated that CAPN1 4751 could serve as an effective marker for genetic selection in crossbred beef cattle and confirmed the efficiency of HRM analysis as a molecular tool for SNP genotyping. In conclusion, the findings provided an alternative approach for SNP detection in livestock breeding programs and represented an important step toward improving meat quality, meeting consumer expectations, and supporting the long-term sustainability of Thailand’s beef industry. The results highlighted the polygenic nature of meat tenderness and emphasized the importance of integrating multiple SNP markers to accurately assess the genetic potential for meat quality traits in cattle. Full article

PIN-LIKES (PILS) auxin transport genes play key roles in plant development, but their functions and molecular mechanism in soybean yield remain unclear. Here, we characterized the 44-member soybean GmPILS genes via comprehensive analyses. Phylogenetic analysis classified GmPILS into three subfamilies, with most proteins being hydrophobic, stable, and membrane-localized. Chromosomal distribution showed random scattering across 17 chromosomes, with gene duplication driving family expansion. Expression profiling identified GmPILS36 and GmPILS40 as seed-specific and differentially expressed between cultivated Suinong14 (SN14) and wild ZYD00006 (ZYD06) soybeans. Population genetic analyses revealed GmPILS40 experienced a domestication bottleneck without yield-related superior haplotypes, while GmPILS36 underwent selection during landrace-to-improved variety domestication. A coding region CC/TT natural variation in GmPILS36 (S/A substitution) was significantly associated with seed weight per plant and 100-seed weight, with the TT genotype conferring superior traits. This study provides insights into GmPILS genes’ evolution and identifies GmPILS36 as an important candidate gene for further functional study and investigation of the molecular mechanisms regulating soybean yield. Full article

Trinidad and Tobago (T&T) has regional influence in the Caribbean, with a diversified economy focused on energy, manufacturing, and finance compared to the tourism-related economies of most of the other Caribbean islands. Notwithstanding, this has not translated into equitable opportunities for all, specifically for people with disabilities. A lack of disaggregated employment data thwarts effective policy planning for people with disabilities. This research sought to examine the barriers to their employment in T&T. Underpinned by the social model of disability, a concurrent mixed-methods approach was employed. Emanating from interviews with people with disabilities and key informants, challenges to employment access, employer perceptions, and apathy emerged as key themes, together with the underlying issues of a lack of legislation and inequitable access to mainstream education. The survey findings indicated that 64% of employers had never employed people with disabilities, 57% expressed neutrality regarding future employment of such individuals, and 69% had not introduced workplace accommodations. A key recommendation of the study is the establishment of an employer resource centre that assists employers in creating and maintaining inclusive workplace accommodations for people with disabilities. This study is the first in Trinidad and Tobago to examine these research objectives from multiple perspectives. Full article

Text-to-Speech (TTS) methods typically employ a sequential approach with an Acoustic Model (AM) and a vocoder, using a Mel spectrogram as an intermediate representation. However, in home environments, TTS systems often struggle with issues such as inadequate robustness against environmental noise and limited adaptability to diverse speaker characteristics. The quality of the Mel spectrogram directly affects the performance of TTS systems, yet existing methods overlook the potential of enhancing Mel spectrogram quality through more comprehensive speech features. To address the complex acoustic characteristics of home environments, this paper introduces AirSpeech, a post-processing model for Mel-spectrogram synthesis. We adopt a Generative Adversarial Network (GAN) to improve the accuracy of Mel spectrogram prediction and enhance the expressiveness of synthesized speech. By incorporating additional conditioning extracted from synthesized audio using specified speech feature parameters, our method significantly enhances the expressiveness and emotional adaptability of synthesized speech in home environments. Furthermore, we propose a global normalization strategy to stabilize the GAN training process. Through extensive evaluations, we demonstrate that the proposed method significantly improves the signal quality and naturalness of synthesized speech, providing a more user-friendly speech interaction solution for smart home applications. Full article

Background: Long pulse width stimulation (LPWS; 120–150 ms) has the potential to stimulate denervated muscles in persons with spinal cord injury (SCI). We examined whether testosterone treatment (TT) + LPWS would increase skeletal muscle size, leg lean mass and improve overall metabolic health in SCI persons with denervation. We hypothesized that one year of combined TT + LPWS would downregulate gene expression of muscle atrophy and upregulate gene expression of muscle hypertrophy and increase mitochondrial health in SCI persons with lower motor neuron (LMN) injury. Methods: Ten SCI participants with chronic LMN injury were randomized into either 12 months, twice weekly, of TT + LPWS (n = 5) or a TT+ standard neuromuscular electrical stimulation (NMES; n = 5). Measurements were conducted at baseline (week 0), 6 months following training (post-intervention 1), and one week following 12 months of training (post-intervention 2). Measurements included body composition assessment using magnetic resonance imaging (MRI) and dual x-ray absorptiometry (DXA). Metabolic profile assessment encompassed measurements of resting metabolic rate, carbohydrate and lipid profiles. Finally, muscle biopsy was captured to measure RNA signaling pathways and mitochondrial oxidative phosphorylation. Results: Compliance and adherence were greater in the TT + NMES compared to the TT + LPWS group. There was a 25% increase in the RF muscle CSA following P1 measurement in the TT + LPWS group. There was a recognizable non-significant decrease in intramuscular fat in both groups. There was a trend (p = 0.07) of decrease in trunk fat mass following TT + LPWS, with an interaction (p = 0.037) in android lean mass between groups. There was a trend (p = 0.08) in mean differences in DXA-visceral adipose tissue (VAT) between groups at P1 measurements. For genes targeting muscle atrophy, TT + LPWS showed a trending decline in MURF1 and FOXO3 genes returning to similar levels as TT + NMES before 12 months. Conclusions: These pilot data demonstrated the safety of applying LPWS in persons with SCI. Six months of TT + LPWS demonstrated increases in rectus femoris muscle CSA. The effects on muscle size were modest between groups. Signaling pathway analysis suggested downregulation of genes involved in muscle atrophy pathways. Future clinical trials may consider a home-based approach with more frequent applications of LPWS. Full article

Cardiovascular diseases have become a leading global health burden, with rising mortality worldwide. WNT and JAK/STAT have been highlighted as emerging biomarkers in cardiovascular disease pathogenesis. This study assessed the Wnt/JAK-STAT signaling pathway in relation to SFRP5 and genetic polymorphisms in cardiac patients. This prospective case–control study included 100 patients with various cardiac diseases (IHD, valvular heart disease, HF, cardiomyopathy, and arrhythmia) and 50 matched healthy controls. Clinical and echocardiographic assessments were performed. Plasma SFRP5, Wnt5a, and JAK levels were measured using ELISA; STAT5A expression by flow cytometry; and SFRP5 (rs780369540) gene polymorphism by TaqMan real-time PCR were also performed in all participants. Cardiac patients showed significantly higher median BMI (33 vs. 28.5 kg/m², p = 0.001) and markedly increased median value of each Wnt5a (16.85 vs. 5.6 pg/mL, p < 0.001), median JAK (9.45 vs. 2.4 pg/mL, p < 0.001), and STAT5A expression (87.55% vs. 33%, p < 0.001), with lower SFRP5 levels (4 vs. 6.7 ng/L, p < 0.001) compared to control. The SFRP5 (rs780369540) T allele was more frequent in patients (51.5% vs. 32%, p = 0.001), and dominant TT + TC genotypes were higher (66% vs. 42%, p = 0.005) compared to the control group. TT carriers showed higher median Wnt5a, lower median SFRP5, and reduced ejection fraction compared to other genotypes (TC, CC) carriers. Multivariate analysis identified elevated Wnt5a, JAK, and decreased SFRP5 as independent predictors of cardiovascular disease (p < 0.05). Cardiac patients showed altered WNT5a, JAK, and SFRP5 levels. SFRP5 polymorphism predicted cardiovascular risk independently. Full article

Background: Individual differences in immune responses to African swine fever virus (ASFV), whether induced by vaccination or natural infection, may be linked to genetic variation in the genes involved in antigen presentation. Methods: A total of nine pigs from the 112-population were selected for RNA-seq analysis. To pinpoint key transcription factors (TFs) regulating gene expression in the lymph nodes, weighted Kendall’s Tau rank correlation analysis was performed to link the TF binding potential with the extent of differential expression of target genes. Results: CD8⁺ T cells expressing a specific epitope of the ASFV p72 protein (ACD8⁺) accounted for 41% of the total CD8⁺ T cells in peripheral blood. A total of 2062 transcripts were identified as differentially expressed across the nine pigs (q-value < 1 × 10⁻⁸). Differential expression levels of the target genes for MECP2, ETS1, ZBTB33, ELK4, and E2F4 were significantly correlated with their TF binding potential (p < 0.05). Six SNPs were identified in the promoter region of ELK4. Analysis of the 112-pig population revealed that SNPs at S.-404A>G and S.-668C>T loci were significantly associated with ACD8⁺ levels (q-value < 0.01). Individuals with the AA genotype at S.-404A>G had significantly higher ACD8⁺ counts compared to those with AG and GG genotypes (q-value < 0.05). At the S.-668C>T locus, ACD8⁺ levels were highest in the CC genotype, followed by CT and TT genotypes, with CC showing notably higher ACD8⁺ counts (q-value < 0.05). Notably, the S.-404A>G site overlaps with potential binding sites for TFs FOXA2, GATAs, and TRPS1, while the S.-668C>T site lies within the binding regions for NR1H3, RARA, VDR, and NR1I3. Conclusion: These mutations may disrupt TFs binding to the ELK4 promoter, potentially reducing ELK4 expression and impairing antigen processing and presentation. Full article

Red walnuts have been widely studied because of their strong antioxidant activity and ornamental value. However, research on the mechanism of anthocyanin biosynthesis in walnuts remains in the initial stage. The regulatory mechanism of TT2-type R2R3-MYB transcription factors in anthocyanin biosynthesis in walnuts is also unclear. Therefore, this study used ‘D2-1’ and ‘Jinghong 1’ walnuts as plant materials. The testa of ‘Jinghong 1’ was red, and its anthocyanin content was significantly higher than that of ‘D2-1’, mainly composed of cyanidin-3-O-arabinoside, cyanidin-3-O-galactoside, and cyanidin-3-O-glucoside. Differentially expressed genes between ‘D2-1’ and ‘Jinghong 1’ testa were enriched in phenylpropanoid biosynthesis and flavonoid biosynthesis. Next, this study identified a TT2-type R2R3-MYB transcription factor JrMYB1L, which was involved in regulating the anthocyanin biosynthesis in the testa of ‘Jinghong 1’. The overexpression of JrMYB1L could promote anthocyanin accumulation in walnut leaves and activate the expression of JrCHS, JrCHI, JrF3H, JrDFR, JrANS, JrUFGT, JrLAR, and JrANR. In addition, yeast two-hybrid results proved that JrMYB1L, JrbHLH42, and JrWD40 proteins could interact with each other. The results of yeast one-hybrid and dual-luciferase assays indicated that JrMYB1L could activate the expression of JrCHS and JrUFGT by binding to their promoters. Based on the above results, this study proposed a possible regulatory mechanism. JrMYB1L activated the expression of JrCHS and JrUFGT in the form of JrMYB1L-JrbHLH42-JrWD40 complex, thereby promoting anthocyanin accumulation in the testa of ‘Jinghong 1’. In summary, this study lays a theoretical foundation for revealing the regulatory mechanism of anthocyanin biosynthesis in red walnut and contributes to the breeding of new varieties of red walnuts with more edible and ornamental value. Full article

The development of parallel audio corpora for Turkic languages, such as Kazakh, Uzbek, and Tatar, remains a significant challenge in the development of multilingual speech synthesis, recognition systems, and machine translation. These languages are low-resource in speech technologies, lacking sufficiently large audio datasets with aligned transcriptions that are crucial for modern recognition, synthesis, and understanding systems. This article presents the development and experimental evaluation of a speech corpus focused on Turkic languages, intended for use in speech synthesis and automatic translation tasks. The primary objective is to create parallel audio corpora using a cascade generation method, which combines artificial intelligence and text-to-speech (TTS) technologies to generate both audio and text, and to evaluate the quality and suitability of the generated data. To evaluate the quality of synthesized speech, metrics measuring naturalness, intonation, expressiveness, and linguistic adequacy were applied. As a result, a multimodal (Kazakh–Turkish, Kazakh–Tatar, Kazakh–Uzbek) corpus was created, combining high-quality natural Kazakh audio with transcription and translation, along with synthetic audio in Turkish, Tatar, and Uzbek. These corpora offer a unique resource for speech and text processing research, enabling the integration of ASR, MT, TTS, and speech-to-speech translation (STS). Full article

Text-to-speech (TTS) systems based on neural networks have undergone a significant evolution, taking a step forward towards achieving human-like quality and expressiveness, which is crucial for applications such as social media content creation and voice interfaces for visually impaired individuals. An entire branch of research, known as Expressive Text-to-speech (ETTS), has emerged to address the so-called one-to-many mapping problem, which limits the naturalness of generated output. However, most ETTS systems applying explicit style modeling treat the prediction of prosodic features as a regressive, rather than generative, process and, consequently, do not capture prosodic diversity. We address this problem by proposing a novel technique for inference-time prediction of speaking-style features, which leverages a diffusion framework for sampling from a learned space of Global Style Tokens-based embeddings, which are then used to condition a neural TTS model. By incorporating the diffusion model, we can leverage its powerful modeling capabilities to learn the distribution of possible stylistic features and, during inference, sample them non-deterministically, which makes the generated speech more human-like by alleviating prosodic monotony across multiple sentences. Our system blends a regressive predictor with a diffusion-based generator to enable smooth control over the diversity of generated speech. Through quantitative and qualitative (human-centered) experiments, we demonstrated that our system generates expressive human speech with non-deterministic high-level prosodic features. Full article

Background: Pediatric patients with beta-thalassemia (BT) face unique immunologic challenges due to chronic transfusions and viral exposure. Hepatitis C virus (HCV), a common infection in polytransfused individuals, may influence immune polarization. However, the combined effect of chronic HCV and host immunogenetics on allergic sensitization remains incompletely understood. Objective: To assess total serum IgE levels and allergic manifestations in HCV-positive vs. HCV-negative BT patients, and explore associations with common polymorphisms in IL10, TLR7, IL4, and IFNG genes Methods: This cross-sectional observational study enrolled 46 BT patients (37 HCV-positive, 9 HCV-negative) and 50 healthy controls. Clinical allergy history, total IgE levels (ELISA), and skin prick tests (SPT) for aeroallergens were collected. Genotyping for IL10 −1082, TLR7 rs179008, IL4 −589, and IFNG +874 polymorphisms was performed. Associations between genotypes, HCV status, and IgE levels were analyzed descriptively due to small sample size Results: HCV-positive BT patients had lower mean IgE levels (18.73 ± 4.2 IU/mL) and fewer reported allergic symptoms (21.6%) compared to HCV-negative counterparts (118.76 ± 7.9 IU/mL; 55.5%). The IL10 −1082 AA and TLR7 rs179008 TT genotypes were more common in the HCV-positive group and were associated with lower IgE levels. No associations were noted for IL4 or IFNG variants. Splenectomy appeared to further modify IgE levels in HCV-negative patients. Due to limited power and absence of multivariate analysis, findings are exploratory. These preliminary observations may inform future studies of immune deviation in chronically infected pediatric cohorts. Conclusions: Chronic HCV infection may contribute to immune tolerance and reduced allergic expression in BT patients, potentially modulated by IL10 and TLR7 genotypes. Further studies with functional immune profiling and larger cohorts are required. Full article

We investigate a combined conservative field, in which classical gravitational and electrostatic sources also exhibit mutual interactions. Hitherto neglected, the coupling between mass and charge may be necessary for constructing a unified conservative force field generated by a single underlying source. We determine the coupling constant of the cross-field components as the geometric mean (G-M) of Newton’s G and Coulomb’s K constants, in both SI units and dimensionless form. Consequently, for two identical objects, the cross-force ($F_{\times }$) is the G-M of the familiar Newton ($F_{\mathrm{g}}$) and Coulomb ($F_{\mathrm{e}}$) forces, so that $F_{\times }=\sqrt{F_{\mathrm{g}}{F}_{\mathrm{e}}}$, where $F_{\mathrm{g}}\ll F_{\times }\ll F_{\mathrm{e}}$. Remarkably, such cross-forces should be measurable in torsion balance experiments involving a suspended neutral mass interacting with a partially ionized gas. Furthermore, we apply our new formulation to estimate the dimensionless amplitude $\parallel {\mathcal{h}}_{\alpha \beta }^{\mathrm{TT}}\parallel$ of gravitational waves that are emitted by inspiraling Reissner–Nordström (RN) black hole binaries, expressed in terms of ratios of the four fundamental lengths of the problem: the distance to the binary D, the binary separation R, the Schwarzschild radius $R_S\propto 2M$ of mass M, and the RN charge (Q) length scale $L_Q\propto 2Q$. In this classical setting with speeds much lower than the speed of light c in vacuum, the surprising appearance of the maximum relativistic tension force $F_{\max }=c^4/\left(4G\right)$ is duly noted. Full article

Bimodal emotion recognition based on audio and text is widely adopted in video-constrained real-world applications such as call centers and voice assistants. However, existing systems suffer from limited cross-domain generalization and monolingual bias. To address these limitations, a cross-lingual bimodal emotion recognition method is proposed, integrating Mamba-based temporal encoders for audio (Wav2Vec2.0) and text (Jina-v3) with a Transformer-based cross-modal fusion architecture (BiFormer). Three corpus-adaptive augmentation strategies are introduced: (1) Stacked Data Sampling, in which short utterances are concatenated to stabilize sequence length; (2) Label Smoothing Generation based on Large Language Model, where the Qwen3-4B model is prompted to detect subtle emotional cues missed by annotators, producing soft labels that reflect latent emotional co-occurrences; and (3) Text-to-Utterance Generation, in which emotionally labeled utterances are generated by ChatGPT-5 and synthesized into speech using the DIA-TTS model, enabling controlled creation of affective audio–text pairs without human annotation. BiFormer is trained jointly on the English Multimodal EmotionLines Dataset and the Russian Emotional Speech Dialogs corpus, enabling cross-lingual transfer without parallel data. Experimental results show that the optimal data augmentation strategy is corpus-dependent: Stacked Data Sampling achieves the best performance on short, noisy English utterances, while Label Smoothing Generation based on Large Language Model better captures nuanced emotional expressions in longer Russian utterances. Text-to-Utterance Generation does not yield a measurable gain due to current limitations in expressive speech synthesis. When combined, the two best performing strategies produce complementary improvements, establishing new state-of-the-art performance in both monolingual and cross-lingual settings. Full article

Preeclampsia (PE), a major cause of maternal and perinatal morbidity, is a hypertensive pregnancy disorder with poorly defined pathogenesis. While dysregulation of core circadian genes including brain and muscle ARNT-like 1 (BMAL1; also termed ARNTL) and circadian locomotor output cycles kaput (CLOCK) has been implicated in PE, the contribution of their genetic polymorphisms to PE remains unclear. In this case–control study, polymorphisms in BMAL1 and CLOCK were genotyped using MassARRAY in 202 PE patients (97 early-onset [eoPE], 105 late-onset [loPE]) and 400 controls. Following genotyping and linkage disequilibrium-pruning (r² > 0.8) to retain representative tag SNPs, the final set for association analysis comprised three non-redundant BMAL1 SNPs (rs4757144, rs11022780, rs969485) and one CLOCK SNP (rs1048004). After confounder adjustment, no significant associations were detected for CLOCK variants, whereas the BMAL1 rs11022780 variant demonstrated a significant protective effect against PE (TT vs. CC: OR = 0.26 [95% CI 0.09–0.78]; recessive model: OR = 0.25 [95% CI 0.09–0.74]), particularly in the eoPE subgroup. Expression quantitative trait locus (eQTL) analysis confirmed that this SNP correlated with BMAL1 mRNA expression in whole blood, and protein–protein interaction analysis highlighted BMAL1′s central role in circadian networks, implying a genetically influenced regulatory mechanism of PE through BMAL1 expression. Full article