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23 pages, 5185 KiB  
Article
Comparative Analysis of the NorSand and HS Small Constitutive Models for Evaluating Static Liquefaction in a Silt Derived from Mine Tailings
by Matias Muñoz-Gaete, Ricardo Gallardo, Edison Atencio, Ricardo Moffat, Pablo F. Parra, Carlos Cacciuttolo and William Araujo
Appl. Sci. 2025, 15(15), 8726; https://doi.org/10.3390/app15158726 (registering DOI) - 7 Aug 2025
Abstract
The representation and assessment of static liquefaction in mine tailings is a significant challenge due to the severe environmental and social damage it can cause. This phenomenon, known for its catastrophic nature, is triggered when the undrained shear strength is exceeded by a [...] Read more.
The representation and assessment of static liquefaction in mine tailings is a significant challenge due to the severe environmental and social damage it can cause. This phenomenon, known for its catastrophic nature, is triggered when the undrained shear strength is exceeded by a static loading stress. In this study, the constitutive models HSS and NS were evaluated to calibrate the experimental curves from an isotropically consolidated undrained (CIU) triaxial test on a low-plasticity silt derived from mine tailings. An axisymmetric model was developed in Plaxis 2D for calibration, followed by a sensitivity analysis of the parameters of both constitutive models, using the RMSE to validate their accuracy. The results indicate that the proposed methodology adequately simulates the experimental curves, achieving an RMSE of 8%. After calibration, a numerical model was implemented to evaluate the propagation of the PFS of a mine tailings storage facility using both models, in terms of excess pore pressures, shear strains, and p’-q diagrams at three control points. The results show that both models are capable of representing the PFS; however, the HSS model reproduces the experimental curves more accurately, establishing itself as an ideal tool for simulating undrained behavior and, consequently, the phenomenon of static liquefaction in mine tailings. Full article
(This article belongs to the Special Issue Mining Engineering: Present and Future Prospectives)
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16 pages, 1481 KiB  
Article
Effects of Underwater Noise Exposure on Early Development in Zebrafish
by Tong Zhou, Yuchi Duan, Ya Li, Wei Yang and Qiliang Chen
Animals 2025, 15(15), 2310; https://doi.org/10.3390/ani15152310 - 7 Aug 2025
Abstract
Anthropogenic noise pollution is a significant global environmental issue that adversely affects the behavior, physiology, and auditory functions of aquatic species. However, studies on the effects of underwater noise on early developmental stages of fish remain scarce, particularly regarding the differential impacts of [...] Read more.
Anthropogenic noise pollution is a significant global environmental issue that adversely affects the behavior, physiology, and auditory functions of aquatic species. However, studies on the effects of underwater noise on early developmental stages of fish remain scarce, particularly regarding the differential impacts of daytime versus nighttime noise exposure. In this study, zebrafish (Danio rerio) embryos were exposed to control group (no additional noise), daytime noise (100–1000 Hz, 130 dB, from 08:00 to 20:00) or nighttime noise (100–1000 Hz, 130 dB, from 20:00 to 08:00) for 5 days, and their embryonic development and oxidative stress levels were analyzed. Compared to the control group, the results indicated that exposure to both daytime and nighttime noise led to delays in embryo hatching time and a significant decrease in larval heart rate. Notably, exposure to nighttime noise significantly increased the larval deformity rate. Noise exposure, particularly at night, elevated the activities of catalase (CAT) and glutathione peroxidase (GPX), as well as the concentration of malondialdehyde (MDA), accompanied by upregulation of antioxidant-related gene expression levels. Nighttime noise exposure significantly increased the abnormality rate of otolith development in larvae and markedly downregulated the expression levels of otop1 related to otolith development regulation, while daytime noise exposure only induced a slight increase in the otolith abnormality rate. After noise exposure, the number of lateral neuromasts in larvae decreased slightly, yet genes (slc17a8 and capgb) related to hair cell development were significantly upregulated. Overall, this study demonstrates that both daytime and nighttime noise can induce oxidative stress and impair embryonic development of zebrafish, with nighttime noise causing more severe damage. Full article
(This article belongs to the Section Animal Physiology)
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19 pages, 17158 KiB  
Article
Deep Learning Strategy for UAV-Based Multi-Class Damage Detection on Railway Bridges Using U-Net with Different Loss Functions
by Yong-Hyoun Na and Doo-Kie Kim
Appl. Sci. 2025, 15(15), 8719; https://doi.org/10.3390/app15158719 (registering DOI) - 7 Aug 2025
Abstract
Periodic visual inspections are currently conducted to maintain the condition of railway bridges. These inspections rely on direct visual assessments by human inspectors, often requiring specialized equipment such as aerial ladders. However, this method is not only time-consuming and costly but also involves [...] Read more.
Periodic visual inspections are currently conducted to maintain the condition of railway bridges. These inspections rely on direct visual assessments by human inspectors, often requiring specialized equipment such as aerial ladders. However, this method is not only time-consuming and costly but also involves significant safety risks. Therefore, there is a growing need for a more efficient and reliable alternative to traditional visual inspections of railway bridges. In this study, we evaluated and compared the performance of damage detection using U-Net-based deep learning models on images captured by unmanned aerial vehicles (UAVs). The target damage types include cracks, concrete spalling and delamination, water leakage, exposed reinforcement, and paint peeling. To enable multi-class segmentation, the U-Net model was trained using three different loss functions: Cross-Entropy Loss, Focal Loss, and Intersection over Union (IoU) Loss. We compared these methods to determine their ability to distinguish actual structural damage from environmental factors and surface contamination, particularly under real-world site conditions. The results showed that the U-Net model trained with IoU Loss outperformed the others in terms of detection accuracy. When applied to field inspection scenarios, this approach demonstrates strong potential for objective and precise damage detection. Furthermore, the use of UAVs in the inspection process is expected to significantly reduce both time and cost in railway infrastructure maintenance. Future research will focus on extending the detection capabilities to additional damage types such as efflorescence and corrosion, aiming to ultimately replace manual visual inspections of railway bridge surfaces with deep-learning-based methods. Full article
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18 pages, 865 KiB  
Review
Proteomics-Based Approaches to Decipher the Molecular Strategies of Botrytis cinerea: A Review
by Olivier B. N. Coste, Almudena Escobar-Niño and Francisco Javier Fernández-Acero
J. Fungi 2025, 11(8), 584; https://doi.org/10.3390/jof11080584 - 6 Aug 2025
Abstract
Botrytis cinerea is a highly versatile pathogenic fungus, causing significant damage across a wide range of plant species. A central focus of this review is the recent advances made through proteomics, an advanced molecular tool, in understanding the mechanisms of B. cinerea infection. [...] Read more.
Botrytis cinerea is a highly versatile pathogenic fungus, causing significant damage across a wide range of plant species. A central focus of this review is the recent advances made through proteomics, an advanced molecular tool, in understanding the mechanisms of B. cinerea infection. Recent advances in mass spectrometry-based proteomics—including LC-MS/MS, iTRAQ, MALDI-TOF, and surface shaving—have enabled the in-depth characterization of B. cinerea subproteomes such as the secretome, surfactome, phosphoproteome, and extracellular vesicles, revealing condition-specific pathogenic mechanisms. Notably, in under a decade, the proportion of predicted proteins experimentally identified has increased from 10% to 52%, reflecting the rapid progress in proteomic capabilities. We explore how proteomic studies have significantly enhanced our knowledge of the fungus secretome and the role of extracellular vesicles (EVs), which play key roles in pathogenesis, by identifying secreted proteins—such as pH-responsive elements—that may serve as biomarkers and therapeutic targets. These technologies have also uncovered fine regulatory mechanisms across multiple levels of the fungal proteome, including post-translational modifications (PTMs), the phosphomembranome, and the surfactome, providing a more integrated view of its infection strategy. Moreover, proteomic approaches have contributed to a better understanding of host–pathogen interactions, including aspects of the plant’s defensive responses. Furthermore, this review discusses how proteomic data have helped to identify metabolic pathways affected by novel, more environmentally friendly antifungal compounds. A further update on the advances achieved in the field of proteomics discovery for the organism under consideration is provided in this paper, along with a perspective on emerging tools and future developments expected to accelerate research and improve targeted intervention strategies. Full article
(This article belongs to the Special Issue Plant Pathogenic Sclerotiniaceae)
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12 pages, 498 KiB  
Article
Virulence of Metarhizium robertsii Strains Isolated from Forest Ecosystems Against Wax Moths (Galleria mellonella, Achroia grisella) and Pine Processionary (Thaumetopoea pityocampa) Larvae
by Spiridon Mantzoukas, Vasileios Papantzikos, Chrysanthi Zarmakoupi, Panagiotis A. Eliopoulos, Ioannis Lagogiannis and George Patakioutas
Biology 2025, 14(8), 1009; https://doi.org/10.3390/biology14081009 - 6 Aug 2025
Abstract
Entomopathogenic fungi (EPF) are one of the most environmentally friendly ways to control a plethora of chewing insects such as T. pityocampa, G. mellonella, and A. grisella. Bioassay of EPF on these highly damaging pests is considered important in the [...] Read more.
Entomopathogenic fungi (EPF) are one of the most environmentally friendly ways to control a plethora of chewing insects such as T. pityocampa, G. mellonella, and A. grisella. Bioassay of EPF on these highly damaging pests is considered important in the face of climate change in order to research alternative solutions that are capable of limiting chemical control, the overuse of which increases insects’ resistance to chemical compounds. In this study, the insecticidal virulence of Metarhizium robertsii isolates, retrieved from forest ecosystems, was tested on second-instar larvae of T. pityocampa, G. mellonella, and A. grisella. Bioassays were carried out in the laboratory, where experimental larvae were sprayed with 2 mL of a six-conidial suspension from each isolate. Mortality was recorded for 144 h after exposure. Mean mortality, lethal concentrations, sporulation percentage, and sporulation time were estimated for each isolate. Metarhizium isolates resulted in the highest mortality (89.2% for G. mellonella and 90.2% for A. grisella). Based on the LC50 estimates determined by the concentration–mortality relationships for the tested fungal isolates, we demonstrated significant virulence on larvae of G. mellonella, A. grisella, and T. pityocampa. Our results indicate that entomopathogenic fungi have the potential to become a very useful tool in reducing chemical applications. Full article
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17 pages, 1750 KiB  
Review
Reproductive Challenges in Ruminants Under Heat Stress: A Review of Follicular, Oocyte, and Embryonic Responses
by Danisvânia Ripardo Nascimento, Venância Antonia Nunes Azevedo, Regislane Pinto Ribeiro, Gabrielle de Oliveira Ximenes, Andreza de Aguiar Silva, Efigênia Cordeiro Barbalho, Laryssa Gondim Barrozo, Sueline Cavalcante Chaves, Maria Samires Martins Castro, Erica Costa Marcelino, Leopoldo Rugieri Carvalho Vaz da Silva, André Mariano Batista and José Roberto Viana Silva
Animals 2025, 15(15), 2296; https://doi.org/10.3390/ani15152296 - 6 Aug 2025
Abstract
This review aims to discuss how heat stress affects ovarian follicles and oocytes, steroidogenesis, and embryo development in ruminants. The literature shows that quiescent primordial follicles appear to be less susceptible to heat stress, but from the primary follicle stage onwards, they begin [...] Read more.
This review aims to discuss how heat stress affects ovarian follicles and oocytes, steroidogenesis, and embryo development in ruminants. The literature shows that quiescent primordial follicles appear to be less susceptible to heat stress, but from the primary follicle stage onwards, they begin to suffer the consequences of heat stress. These adverse effects are exacerbated when the follicles are cultured in vitro. In antral follicles, heat stress reduces granulosa cell viability and proliferation in both in vivo and in vitro models. Oocyte maturation, both nuclear and cytoplasmic, is also compromised, and embryo quality declines under elevated thermal conditions. These effects are linked to intracellular disturbances, including oxidative imbalance, mitochondrial dysfunction, and altered hormonal signaling. The differences between in vivo and in vitro responses reflect the complexity of the biological impact of heat stress and emphasize the protective role of the physiological microenvironment. A better understanding of how heat stress alters the function of ovarian follicles, oocytes, and embryos is crucial. This knowledge is critical to devise effective strategies that mitigate damage, support fertility, and improve outcomes in assisted reproduction for livestock exposed to high environmental temperatures. Full article
(This article belongs to the Special Issue Heat Stress in Animal Oocytes: Impacts, Evaluation, and Alleviation)
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17 pages, 2538 KiB  
Article
Influence of Abrasive Flow Rate and Feed Rate on Jet Lag During Abrasive Water Jet Cutting of Beech Plywood
by Monika Sarvašová Kvietková, Ondrej Dvořák, Chia-Feng Lin, Dennis Jones, Petr Ptáček and Roman Fojtík
Appl. Sci. 2025, 15(15), 8687; https://doi.org/10.3390/app15158687 (registering DOI) - 6 Aug 2025
Abstract
Cutting beech plywood using abrasive water jet (AWJ) technology represents a significant area of research due to increasing demands for precision, quality, and environmental sustainability in manufacturing processes within the woodworking industry. AWJ technology enables non-contact cutting of materials without causing thermal deformation [...] Read more.
Cutting beech plywood using abrasive water jet (AWJ) technology represents a significant area of research due to increasing demands for precision, quality, and environmental sustainability in manufacturing processes within the woodworking industry. AWJ technology enables non-contact cutting of materials without causing thermal deformation or mechanical damage, which is crucial for preserving the structural integrity and mechanical properties of the plywood. This article investigates cutting beech plywood using technical methods using an abrasive water jet (AWJ) at 400 MPa pressure, with Australian garnet (80 MESH) as the abrasive material. It examines how abrasive mass flow rate, traverse speed, and material thickness affect AWJ lag, which in turn influences both cutting quality and accuracy. Measurements were conducted with power abrasive mass flow rates of 250, 350, and 450 g/min and traverse speeds of 0.2, 0.4, and 0.6 m/min. Results show that increasing the abrasive mass flow rate from 250 g/min to 350 g/min slightly decreased the AWJ cut width by 0.05 mm, while further increasing to 450 g/min caused a slight increase of 0.1 mm. Changes in traverse speed significantly influenced cut width; increasing the traverse speed from 0.2 m/min to 0.4 m/min widened the AWJ by 0.21 mm, while increasing it to 0.6 m/min caused a slight increase of 0.18 mm. For practical applications, it is recommended to use an abrasive mass flow rate of around 350 g/min combined with a traverse speed between 0.2 and 0.4 m/min when cutting beech plywood with AWJ. This balance minimizes jet lag and maintains high surface quality comparable to conventional milling. For thicker plywood, reducing the traverse speed closer to 0.2 m/min and slightly increasing the abrasive flow should ensure clean cuts without compromising surface integrity. Full article
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14 pages, 5840 KiB  
Article
Paint Removal Performance and Sub-Surface Microstructural Evolution of Ti6Al4V Alloy Using Different Process Parameters of Continuous Laser Cleaning
by Haoye Zeng, Biwen Li, Liangbin Hu, Yun Zhang, Ruiqing Li, Chaochao Zhou and Pinghu Chen
Coatings 2025, 15(8), 916; https://doi.org/10.3390/coatings15080916 (registering DOI) - 6 Aug 2025
Abstract
Laser cleaning technology has been increasingly applied in the removal of damaged protective coatings from aircraft components due to its environmental friendliness and high efficiency. Appropriate laser cleaning process parameters improve cleaning efficiency while preventing substrate damage. In this study, a Gaussian continuous-wave [...] Read more.
Laser cleaning technology has been increasingly applied in the removal of damaged protective coatings from aircraft components due to its environmental friendliness and high efficiency. Appropriate laser cleaning process parameters improve cleaning efficiency while preventing substrate damage. In this study, a Gaussian continuous-wave laser was used to remove the 120 μm coating on the surface of Ti6Al4V alloy. The influence of laser power (100 W to 200 W) and scanning speed (520 mm/min to 610 mm/min) on the paint removal effect was explored based on paint removal rate, surface roughness, microstructural evolution, and the hardness’ change in the direction of heat transfer. The results reveal that optimal paint removal parameters are achieved at a laser power of 100 W with a scanning speed of 550 mm/min. The surface roughness of the sample after paint removal (55 nm) is similar to that of the original substrate (56 nm). Through EBSD analysis, the influence of laser thermal accumulation on the microstructure of the substrate is relatively small. The average hardness of the cross-section after cleaning was 347 HV, which was only 3.41% higher than that of the original substrate. This confirms that parameter-controlled laser cleaning can effectively remove ~120 μm thick paint layers without inflicting damage on the substrate. Full article
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19 pages, 3596 KiB  
Article
Radon Exposure to the General Population of the Fernald Community Cohort
by John F. Reichard, Swade Barned, Angelico Mendy and Susan M. Pinney
Atmosphere 2025, 16(8), 939; https://doi.org/10.3390/atmos16080939 (registering DOI) - 5 Aug 2025
Abstract
The Fernald Feed Materials Production Center (FMPC), located in Fernald, Ohio, USA, released radon (Rn) as a byproduct of the processing of uranium materials during the years from 1951 to 1989. Rn is a colorless, odorless gas that emits charged alpha radiation that [...] Read more.
The Fernald Feed Materials Production Center (FMPC), located in Fernald, Ohio, USA, released radon (Rn) as a byproduct of the processing of uranium materials during the years from 1951 to 1989. Rn is a colorless, odorless gas that emits charged alpha radiation that interacts with cells in the lung and trachea-bronchial tree, leading to DNA damage, mutations, and tumor initiation. The purpose of this project was to use evidence collected by the Fernald Dosimetry Reconstruction Project and other sources to estimate the outdoor Rn exposure to individuals in the community immediately surrounding the FMPC during the years of plant operation. Using previously tabulated source terms, diffusion and meteorological data, and self-reported detailed residential histories, we estimated radon exposure for approximately 9300 persons who lived at more than 14,000 addresses. The results indicated that a portion of the population cohort experiences mean annual Rn exposure exceeding the U.S. Environmental Protection Agency (EPA) action limit of 4 pCiL−1. These exposure estimates support the analysis of the incidence of lung cancer in the Fernald Community Cohort (FCC). Full article
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18 pages, 1602 KiB  
Article
Interacting Effects of Heat and Nanoplastics Affect Wheat (Triticum turgidum L.) Seedling Growth and Physiology
by Debora Fontanini, Stefania Bottega, Monica Ruffini Castiglione and Carmelina Spanò
Plants 2025, 14(15), 2426; https://doi.org/10.3390/plants14152426 - 5 Aug 2025
Abstract
Nano- and microplastic pollution, together with the ongoing rise in global temperatures driven by climate change, represent increasingly critical environmental challenges. Although these stressors often co-occur in the environment, their combined effects on plant systems remain largely unexplored. To test the hypothesis that [...] Read more.
Nano- and microplastic pollution, together with the ongoing rise in global temperatures driven by climate change, represent increasingly critical environmental challenges. Although these stressors often co-occur in the environment, their combined effects on plant systems remain largely unexplored. To test the hypothesis that their interaction may exacerbate the effects observed under each stressor individually, we investigated the response of seedlings of Triticum turgidum to treatments with fluorescent polystyrene nanoplastics under optimal (25 °C) and elevated (35 °C) temperature conditions. We evaluated seedling growth, photosynthetic pigment content, and oxidative stress markers using both biochemical and histochemical techniques. In addition, we assessed enzymatic and non-enzymatic antioxidant responses. The use of fluorescently labeled nanoplastics enabled the visualization of their uptake and translocation within plant tissues. Elevated temperatures negatively affect plant growth, increasing the production of proline, a key protective molecule, and weakly activating secondary defense mechanisms. Nanoplastics disturbed wheat seedling physiology, with these effects being amplified under high temperature conditions. Combined stress enhances nanoplastic uptake in roots, increases oxidative damage, and alters antioxidant responses, reducing defense capacity in leaves while triggering compensatory mechanisms in roots. These findings underscore a concerning interaction between plastic pollution and climate warming in crop plants. Full article
(This article belongs to the Section Plant Physiology and Metabolism)
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24 pages, 11081 KiB  
Article
Quantifying Wildfire Dynamics Through Spatio-Temporal Clustering and Remote Sensing Metrics: The 2023 Quebec Case Study
by Tuğrul Urfalı and Abdurrahman Eymen
Fire 2025, 8(8), 308; https://doi.org/10.3390/fire8080308 - 5 Aug 2025
Viewed by 60
Abstract
Wildfires have become increasingly frequent and destructive environmental hazards, especially in boreal ecosystems facing prolonged droughts and temperature extremes. This study presents an integrated spatio-temporal framework that combines Spatio-Temporal Density-Based Spatial Clustering of Applications with Noise (ST-DBSCAN), Fire Radiative Power (FRP), and the [...] Read more.
Wildfires have become increasingly frequent and destructive environmental hazards, especially in boreal ecosystems facing prolonged droughts and temperature extremes. This study presents an integrated spatio-temporal framework that combines Spatio-Temporal Density-Based Spatial Clustering of Applications with Noise (ST-DBSCAN), Fire Radiative Power (FRP), and the differenced Normalized Burn Ratio (ΔNBR) to characterize the dynamics and ecological impacts of large-scale wildfires, using the extreme 2023 Quebec fire season as a case study. The analysis of 80,228 VIIRS fire detections resulted in 19 distinct clusters across four fire zones. Validation against the National Burned Area Composite (NBAC) showed high spatial agreement in densely burned areas, with Intersection over Union (IoU) scores reaching 62.6%. Gaussian Process Regression (GPR) revealed significant non-linear relationships between FRP and key fire behavior metrics. Higher mean FRP was associated with both longer durations and greater burn severity. While FRP was also linked to faster spread rates, this relationship varied by zone. Notably, Fire Zone 2 exhibited the most severe ecological impact, with 83.8% of the area classified as high-severity burn. These findings demonstrate the value of integrating spatial clustering, radiative intensity, and post-fire vegetation damage into a unified analytical framework. Unlike traditional methods, this approach enables scalable, hypothesis-driven assessment of fire behavior, supporting improved fire management, ecosystem recovery planning, and climate resilience efforts in fire-prone regions. Full article
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17 pages, 826 KiB  
Review
Mechanisms and Impact of Acacia mearnsii Invasion
by Hisashi Kato-Noguchi and Midori Kato
Diversity 2025, 17(8), 553; https://doi.org/10.3390/d17080553 - 4 Aug 2025
Viewed by 69
Abstract
Acacia mearnsii De Wild. has been introduced to over 150 countries for its economic value. However, it easily escapes from plantations and establishes monospecific stands across plains, hills, valleys, and riparian habitats, including protected areas such as national parks and forest reserves. Due [...] Read more.
Acacia mearnsii De Wild. has been introduced to over 150 countries for its economic value. However, it easily escapes from plantations and establishes monospecific stands across plains, hills, valleys, and riparian habitats, including protected areas such as national parks and forest reserves. Due to its negative ecological impact, A. mearnsii has been listed among the world’s 100 worst invasive alien species. This species exhibits rapid stem growth in its sapling stage and reaches reproductive maturity early. It produces a large quantity of long-lived seeds, establishing a substantial seed bank. A. mearnsii can grow in different environmental conditions and tolerates various adverse conditions, such as low temperatures and drought. Its invasive populations are unlikely to be seriously damaged by herbivores and pathogens. Additionally, A. mearnsii exhibits allelopathic activity, though its ecological significance remains unclear. These characteristics of A. mearnsii may contribute to its expansion in introduced ranges. The presence of A. mearnsii affects abiotic processes in ecosystems by reducing water availability, increasing the risk of soil erosion and flooding, altering soil chemical composition, and obstructing solar light irradiation. The invasion negatively affects biotic processes as well, reducing the diversity and abundance of native plants and arthropods, including protective species. Eradicating invasive populations of A. mearnsii requires an integrated, long-term management approach based on an understanding of its invasive mechanisms. Early detection of invasive populations and the promotion of public awareness about their impact are also important. More attention must be given to its invasive traits because it easily escapes from cultivation. Full article
(This article belongs to the Special Issue Plant Adaptation and Survival Under Global Environmental Change)
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35 pages, 698 KiB  
Review
Mechanistic Role of Heavy Metals in Driving Antimicrobial Resistance: From Rhizosphere to Phyllosphere
by Rahul Kumar, Tanja P. Vasić, Sanja P. Živković, Periyasamy Panneerselvam, Gustavo Santoyo, Sergio de los Santos Villalobos, Adeyemi Nurudeen Olatunbosun, Aditi Pandit, Leonard Koolman, Debasis Mitra and Pankaj Gautam
Appl. Microbiol. 2025, 5(3), 79; https://doi.org/10.3390/applmicrobiol5030079 - 4 Aug 2025
Viewed by 71
Abstract
Heavy metal pollution represents a pervasive environmental challenge that significantly exacerbates the ever-increasing crisis of antimicrobial resistance and the capacity of microorganisms to endure and proliferate despite antibiotic interventions. This review examines the intricate relationship between heavy metals and AMR, with an emphasis [...] Read more.
Heavy metal pollution represents a pervasive environmental challenge that significantly exacerbates the ever-increasing crisis of antimicrobial resistance and the capacity of microorganisms to endure and proliferate despite antibiotic interventions. This review examines the intricate relationship between heavy metals and AMR, with an emphasis on the underlying molecular mechanisms and ecological ramifications. Common environmental metals, including arsenic, mercury, cadmium, and lead, exert substantial selective pressures on microbial communities. These induce oxidative stress and DNA damage, potentially leading to mutations that enhance antibiotic resistance. Key microbial responses include the overexpression of efflux pumps that expel both metals and antibiotics, production of detoxifying enzymes, and formation of protective biofilms, all of which contribute to the emergence of multidrug-resistant strains. In the soil environment, particularly the rhizosphere, heavy metals disrupt plant–microbe interactions by inhibiting beneficial organisms, such as rhizobacteria, mycorrhizal fungi, and actinomycetes, thereby impairing nutrient cycling and plant health. Nonetheless, certain microbial consortia can tolerate and detoxify heavy metals through sequestration and biotransformation, rendering them valuable for bioremediation. Advances in biotechnology, including gene editing and the development of engineered metal-resistant microbes, offer promising solutions for mitigating the spread of metal-driven AMR and restoring ecological balance. By understanding the interplay between metal pollution and microbial resistance, we can more effectively devise strategies for environmental protection and public health. Full article
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30 pages, 1511 KiB  
Review
Environmental and Health Impacts of Pesticides and Nanotechnology as an Alternative in Agriculture
by Jesús Martín Muñoz-Bautista, Ariadna Thalía Bernal-Mercado, Oliviert Martínez-Cruz, Armando Burgos-Hernández, Alonso Alexis López-Zavala, Saul Ruiz-Cruz, José de Jesús Ornelas-Paz, Jesús Borboa-Flores, José Rogelio Ramos-Enríquez and Carmen Lizette Del-Toro-Sánchez
Agronomy 2025, 15(8), 1878; https://doi.org/10.3390/agronomy15081878 - 3 Aug 2025
Viewed by 244
Abstract
The extensive use of conventional pesticides has been a fundamental strategy in modern agriculture for controlling pests and increasing crop productivity; however, their improper application poses significant risks to human health and environmental sustainability. This review compiles scientific evidence linking pesticide exposure to [...] Read more.
The extensive use of conventional pesticides has been a fundamental strategy in modern agriculture for controlling pests and increasing crop productivity; however, their improper application poses significant risks to human health and environmental sustainability. This review compiles scientific evidence linking pesticide exposure to oxidative stress and genotoxic damage, particularly affecting rural populations and commonly consumed foods, even at levels exceeding the maximum permissible limits in fruits, vegetables, and animal products. Additionally, excessive pesticide use has been shown to alter soil microbiota, negatively compromising long-term agricultural fertility. In response to these challenges, recent advances in nanotechnology offer promising alternatives. This review highlights the development of nanopesticides designed for controlled release, improved stability, and targeted delivery of active ingredients, thereby reducing environmental contamination and increasing efficacy. Moreover, emerging nanobiosensor technologies, such as e-nose and e-tongue systems, have shown potential for real-time monitoring of pesticide residues and soil health. Although pesticides are still necessary, it is crucial to implement stricter laws and promote sustainable solutions that ensure safe and responsible agricultural practices. The need for evidence-based public policy is emphasized to regulate pesticide use and protect both human health and agricultural resources. Full article
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16 pages, 3316 KiB  
Article
Experimental Study on the Electromagnetic Forming Behavior of Pre-Painted Al 99.0 Sheet
by Dorin Luca, Vasile Șchiopu and Dorian D. Luca
J. Manuf. Mater. Process. 2025, 9(8), 259; https://doi.org/10.3390/jmmp9080259 - 3 Aug 2025
Viewed by 203
Abstract
Development of forming methods for surface-coated metals is a current concern due to their economic and environmental advantages. For a successful forming operation, it is necessary that both components, the substrate and the coating, are able to withstand stress without damage until the [...] Read more.
Development of forming methods for surface-coated metals is a current concern due to their economic and environmental advantages. For a successful forming operation, it is necessary that both components, the substrate and the coating, are able to withstand stress without damage until the final shape and dimensions are reached. This goal can be achieved through good knowledge of the elastic and plastic properties of the substrate and the coating, the compatibility between them, the appropriate surface treatment, and the rigorous control of technological forming parameters. Our study was carried out with flat specimens of pre-painted Al 99.0 sheet that were electromagnetically formed by bulging. Forming behavior was investigated as depending on the initial thickness of the substrate, on the aluminum sheet pretreatment, as well as on the plastic deformation path of the metal–paint structure. To verify the damage to the paint layer, tests with increasing strains were performed, and the interface between the metal and the coating layer was investigated by scanning electron microscopy. The obtained results indicate that electromagnetic forming of pre-painted sheets can be a feasible method for specific applications if the forming degree of the substrate is tightly correlated with the type of desired coating and with the pretreatment method used for the metal surface. Full article
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