Search Results (5,996)

Pediatric food allergies are an escalating public health concern, with nut allergies representing a primary cause of persistent hypersensitivity and anaphylaxis. New data suggests that pediatric populations with multiple nut allergies (MNA) may be at higher anaphylaxis risk than their counterparts with single nut allergies. Despite this, there is an absence of literature posing multiple nut allergies against singular nut allergy cases. The majority of the research in this topic is directed towards singular nut allergy, without any differentiation between children with one versus multiple sensitivities. Epidemiological evidence indicates that multiple nut allergies are associated with lifelong sensitization, high cross-reactivity potential and increased risk and severity of reactions. Compounding clinical risk factors reinforce the already high risk associated with MNA and indicate that these children require careful monitoring and individual management. Diagnostic tools, including component-resolved diagnostics and oral food challenges, enable differentiation between true multi-nut sensitization and cross-reactivity, guiding targeted interventions. Management strategies must therefore be multifaceted, encompassing selective allergen avoidance, emergency preparedness with epinephrine auto-injectors, asthma control, nutritional support, and psychosocial care. Recognizing MNA as a distinct, high-risk phenotype highlights the necessity of precision-based, biomarker-driven clinical approaches to optimize safety, reduce morbidity, and improve quality of life for affected pediatric populations. Full article

The aim of this study was to investigate the molecular interactions of heparin, diclofenac, and their supramolecular complexes with cyclooxygenase enzymes (COX-1 and COX-2) using computational docking techniques. Diclofenac is a widely used nonsteroidal anti-inflammatory drug (NSAID) that inhibits COX isoforms, whereas heparin is a polyanionic glycosaminoglycan with established anticoagulant and emerging anti-inflammatory properties. Supramolecular association between these agents may modulate their physicochemical behavior and target engagement. Molecular modeling, dual-drug docking, and molecular dynamics (MD) simulations were employed to characterize the interactions of heparin, diclofenac, and pre-formed heparin–diclofenac complexes with COX-1 and COX-2. Geometry optimization and lipophilicity (logP) estimates were obtained using HyperChem, while protein–ligand docking was performed in HEX using crystallographic COX structures from the Protein Data Bank. Docking poses were analyzed in Chimera, and selected complexes were refined through short MD simulations. Pre-formed heparin–diclofenac assemblies exhibited markedly enhanced docking scores toward both COX isoforms compared with single ligands. Binding orientation strongly influenced affinity: for COX-1, the heparin–diclofenac configuration yielded the most favorable interaction, whereas for COX-2 the diclofenac–heparin configuration was preferred. Both assemblies adopted binding modes distinct from free diclofenac, suggesting cooperative electrostatic and hydrophobic contacts at the enzyme surface. Supramolecular complexation also altered calculated logP values relative to the individual compounds. MD simulations supported the relative stability of the top-ranked complex–COX assemblies. These findings indicate that heparin–diclofenac assemblies may enhance and reorganize predicted COX interactions in a configuration-dependent manner and illustrate the utility of dual-drug docking for modeling potential synergistic effects. Such insights may inform the design of localized or topical formulations, potentially incorporating non-anticoagulant heparin derivatives, to achieve effective COX inhibition with reduced systemic exposure. However, the results rely on simplified heparin fragments, legacy docking tools, and short MD simulations, and should therefore be interpreted qualitatively. Experimental studies will be essential to confirm whether such supramolecular assemblies form under physiological conditions and whether they influence COX inhibition in vivo. Full article

Curcumin is a naturally occurring polyphenol that has gained attention in cancer research due to its anti-inflammatory, antioxidant, and anticancer properties. However, its clinical use is limited due to poor water solubility, rapid degradation, and low bioavailability, which reduce its therapeutic effectiveness. To overcome these issues, curcumin has been combined with other agents, including chemotherapeutic drugs, photothermal materials, and metal-based compounds, to improve stability and antitumor activity. Biocompatible drug-delivery systems that allow controlled or sustained release are particularly valuable in oncology, as they can minimize side effects and improve treatment efficiency. Among these carriers, metal–organic frameworks (MOFs) have emerged as promising platforms due to their porous structure, tunable chemistry, and high loading capacity. This review focuses on the potential of MOFs as nanocarriers for curcumin, emphasizing their ability to enhance stability, increase bioavailability, improve therapeutic outcomes, and deliver the drug selectively to tumor sites. Full article

White-matter development during fetal life represents one of the most vulnerable processes to environmental disruption, as it relies on the precisely timed proliferation, migration, and differentiation of oligodendrocyte lineage cells. Among environmental threats, exposure to toxic compounds contained in tobacco smoke and vaping aerosols represents a major yet preventable risk during pregnancy. Despite growing awareness, tobacco smoking remains widespread, and a substantial proportion of the population—including pregnant women—continues to perceive electronic nicotine delivery systems (ENDS) as less harmful, a misconception that contributes to persistent prenatal exposure. These products expose the fetus to numerous substances that readily cross the placenta and reach the developing brain, including compounds with endocrine-disrupting activity, where they interfere with white-matter development. Epidemiological and neuroimaging studies consistently reveal microstructural alterations in white matter that correlate with long-term cognitive and behavioral impairments in offspring exposed in utero. These alterations may arise from both nicotine-specific pathways and the actions of other toxicants in cigarette smoke and ENDS aerosols that cross the placenta and disrupt white-matter emergence and maturation. Preclinical research provides mechanistic insight: nicotine acts directly on nicotinic acetylcholine receptors (nAChRs) in oligodendrocyte precursor cells, disrupting calcium signaling and differentiation, while additional constituents of smoke and vaping aerosols also affect astrocyte and microglial function and disturb the extracellular milieu required for proper myelination. Full article

Bone is a structurally complex and dynamic tissue that plays a crucial role in mobility and skeletal stability. However, conditions such as osteoporosis, osteoarthritis, trauma-induced fractures, infections, and malignancies often necessitate the use of orthopedic and dental implants. Despite significant progress in implant biomaterials, challenges such as bacterial infection, inflammation, and loosening continue to compromise implant longevity, frequently leading to revision surgeries and extended recovery times. Smart coatings have emerged as a next-generation solution to these problems by providing on-demand, localized therapeutic responses to microenvironmental changes around implants and promoting bone regeneration. Such coatings can minimize antibiotic resistance by enabling controlled, stimulus-triggered drug release. Although the idea of using pH-sensitivity as a tool to make smart coatings is not a new thought, there are no options currently good enough to enter clinical studies. This review provides a comprehensive overview of recent advances in pH-sensitive polymers, hybrid composites, porous architectures, and bioactive linkers designed to dynamically respond to pathological pH variations at implant sites. By investigating the mechanisms of action, antibacterial and anti-inflammatory effects, and roles in bone regeneration, it is shown that the ability to provide time-dependent drug release for both short-term and long-term infections, as well as keeping the environment welcoming to the bone cell growth and replacement, is not an easy goal to reach, even with a fully biocompatable, non-toxic, and semi-biodegradable (one that releases the drug, but does not fade away) coating material compound. Reviewing all available options, including their functions and failures, finally, emerging trends, translational barriers, and future opportunities for clinical implementation are highlighted, underscoring the transformative potential of bioresponsive coatings in orthopedic and dental implant technologies. Full article

Breast cancer remains the most frequently diagnosed malignant tumor among women worldwide, and the limited selectivity as well as the emerging resistance to currently used therapies highlight the need to search for new therapeutic compounds. Aromatase, a key enzyme in the estrogen biosynthesis pathway, represents a recognized molecular target in the treatment of hormone-dependent cancers. In this study, six new 1,3,4-thiadiazole derivatives containing two halogen-substituted aromatic rings were designed and synthesized as potential nonsteroidal aromatase inhibitors. The cytotoxic activity of the obtained compounds was evaluated against two breast cancer cell lines: MCF-7 (estrogen-dependent) and MDA-MB-231 (estrogen-independent). All tested compounds exhibited concentration-dependent cytotoxic activity against MCF-7 cells, with the strongest effects observed for compounds A2, A3, B1, and B3 (IC₅₀ ≈ 52–55 µM). In contrast, none of the tested compounds showed significant activity against MDA-MB-231 cells (IC₅₀ > 100 µM), suggesting their selectivity toward estrogen-dependent cancer cells. Compound B3, identified as the most promising, was further subjected to in silico analyses. Molecular docking and molecular dynamics simulations revealed that B3 occupies a binding site similar to that of the co-crystallized native inhibitor and forms interactions characteristic of strong aromatase inhibitors. The obtained results confirm a mechanism of action related to aromatase inhibition and indicate that fluorophenyl-substituted 1,3,4-thiadiazole derivatives represent a promising scaffold for the design of new, selective, and less toxic aromatase inhibitors. Full article

Sulfur compounds in fossil fuels pose significant environmental and industrial challenges, creating a demand for efficient and sustainable desulfurization strategies. Among the available techniques, adsorptive desulfurization has emerged as a promising approach due to its operational simplicity and low energy requirements. In this study, a Ni–Cr modified ZSM-5 zeolite was synthesized to enhance the removal of dibenzothiophene (DBT) from model fuel. The catalyst was prepared by incorporating varying metal loadings and evaluated to identify optimal performance. Structural and chemical characterizations, including FESEM, XRD, NH₃-TPD, FTIR, EDS, and BET analyses, confirmed the successful integration of nickel and chromium within the zeolite framework and demonstrated improved acidity and surface features favorable for adsorption. The catalyst containing 3% chromium and 5% nickel exhibited the highest activity, removing approximately 76% of DBT. Moreover, the optimized material maintained its adsorption efficiency over three consecutive reuse cycles, indicating strong stability and regeneration capability. Overall, the results demonstrate that Ni–Cr/ZSM-5 is a promising and sustainable adsorbent for sulfur removal applications and offers valuable potential for cleaner fuel processing technologies. Full article

This study aims to develop a novel high-efficiency lure for Tomicus yunnanensis Existing bark beetle attractants often rely on single or fixed-ratio blends of host volatiles and their oxidation products, which struggle to mimic the dynamic release process of insect semiochemicals in nature. To address this, we established a dynamic reaction system based on the catalytic oxidation of α-pinene: ① background control (no catalyst, no heating), ② thermal oxidation system (no catalyst, 40 °C), and ③ catalytic oxidation system (with a titanium–copper modified chabazite-type zeolite catalyst, 40 °C). Behavioral screening using a Y-tube olfactometer revealed a clear gradient in attraction effectiveness among the three systems: catalytic oxidation > thermal oxidation > background control. The products from the catalytic oxidation system at 2 h of reaction showed the highest efficacy, achieving an attraction rate of 61%, which was significantly superior to the α-pinene control. These results indicate that generating dynamically proportioned volatile mixtures through catalytic oxidation can significantly enhance the attraction of T. yunnanensis Further analysis by gas chromatography–mass spectrometry (GC-MS) demonstrated that the catalyst efficiently promoted the directional conversion of α-pinene into key bioactive compounds such as verbenol, myrtenal, and myrtenone, thereby substantially improving behavioral activity. After field validation, this dynamically released attractant could potentially be developed into a real-time field-release lure system for monitoring adult emergence and large-scale trapping, providing a feasible new technological pathway for the precise and sustained management of bark beetle pests. Full article

Blueberry (Vaccinium spp.) is a high-value crop due to its growing global demand, recognized nutraceutical properties, and strong linkage with emerging technologies in precision agriculture and postharvest management. To characterize the scientific evolution and intellectual structure, we conducted a bibliometric analysis of 474 documents indexed in Scopus between 1987 and 2025. A systematic search strategy based on taxonomic, agronomic, and technological descriptors was applied, followed by data cleaning and analysis with Bibliometrix and VOSviewer. Performance indicators and science-mapping techniques were used to examine temporal growth, geographical distribution, institutional and author leadership, and thematic structure. Scientific output shows a sustained upward trend with a maximum of 42 articles in 2024, confirming the consolidation of blueberry as a model crop for interdisciplinary research. Research articles represent over 75% of the total (359/474), evidencing an application-oriented and experimentally grounded field. Agricultural and Biological Sciences dominate (382 documents), followed by Engineering (70) and Biochemistry, Genetics, and Molecular Biology (66), reflecting increasing integration of crop management, technological innovation, and food science. Thematic mapping identified five main clusters: physiology and health, plant protection, agronomic management and digitalization, processing and stability of phenolic compounds, and analytical characterization. The analysis reveals gaps in the integration of physiology, food science, and metabolomics, as well as in the biological validation of biomarkers and the study of peripheral Vaccinium species. Overall, the field exhibits a consolidated and sustainability-oriented interdisciplinarity, highlighting opportunities to advance toward more comparable analytical protocols, digital traceability, and artificial-intelligence-assisted decision support along the blueberry value chain. Full article

Water scarcity is fostering an urgent need to drive research into novel and synergistic water treatment approaches, with advanced oxidation processes (AOPs) emerging as a superior option for treating various contaminants. The spread of vinyl chloride (VC) through groundwater sources raises concerns for potable water production due to its toxic and carcinogenic properties. This study integrates ozone-based degradation experiments with data-driven modelling approaches to statistically characterize and predict VC removal under different water-matrix conditions. Ozonation alone enables partial removal of VC from two contaminated groundwater samples, while integration of O₃/H₂O₂ treatment further enhances the degradation efficacy (70–97%). Decreasing VC concentration below the parametric value of 0.5 µg/L requires application of the peroxone process or photodegradation by O₃/H₂O₂/UV for groundwater with higher levels of interfering compounds. Advanced machine learning models and ensemble methods were also tested to enhance predictive accuracy for target molecule degradation, considering water characteristics and treatment parameters as input features. An ensemble of Random Forest and Neural Network predictions yielded the best performance (R² = 0.99; Mean Squared Error = 10.8), demonstrating the effectiveness of ensemble approaches for complex chemical prediction tasks and highlighting areas for further refinement to improve interpretability and predictive consistency of AOP treatment outcomes. This study not only aligns with the current momentum in AI-assisted AOP research but also advances it by delivering a generalizable, reproducible, and interpretable ensemble model trained on experimentally diverse datasets. Full article

Meat provides high-quality protein and essential micronutrients such as vitamin B12, heme iron, zinc, and selenium, along with conditionally essential compounds including creatine, carnitine, and taurine. Growing concerns over environmental sustainability, animal welfare, and potential health risks associated with excessive meat consumption have spurred the development of plant-based and cultured alternatives intended to replicate the nutritional and sensory attributes of meat. This review critically examines the extent to which these emerging products achieve nutrient equivalence with conventional meat, focusing on essential and conditionally essential nutrients, their bioavailability, and implications for human health. After outlining the physiological importance of nutrients characteristically supplied by meat, the review compares the composition of plant-based meat analogs (PBMAs) and cultured meat prototypes. Differences in fortification strategies, ingredient formulation, and the presence of anti-nutritional factors are discussed in relation to nutrient absorption and utilization. Current PBMAs can approximate protein content but generally provide lower levels and reduced bioavailability of vitamin B12, heme iron, creatine, taurine, and long-chain omega-3 fatty acids unless fortified. Cultured meat offers theoretical potential for compositional optimization through cellular engineering but remains limited by scarce empirical data. Achieving nutrient equivalence with conventional meat thus represents a major scientific, technological, and regulatory challenge. Future progress will depend on integrating nutritional design into product development, validating bioavailability in human studies, and implementing transparent labeling to ensure that next-generation meat alternatives meet both health and sustainability goals. Full article

Whey, a by-product of cheese and casein manufacture, represents a major output in dairy processing and a valuable resource for the production of functional foods. This review examines the technological, environmental, and nutritional aspects of whey valorization, emphasizing its transformation from an ecological burden to a raw material with high economic potential. Over time, whey has evolved from being regarded as waste product to becoming a strategic ingredient in the formulation of modern functional foods and bio-based materials. Data from January 2015 to October 2025 were collected from PubMed, Web of Science, and Scopus to outline global whey production, utilization rates, and emerging processing methods. Modern membrane, enzymatic, and non-thermal technologies enable the recovery of valuable components, including proteins, lactose, and bioactive compounds. The use of these techniques reduces the biochemical and chemical oxygen demand in wastewater The review highlights the use of whey in functional beverages, milk and meat processing, edible films, bioplastics, and biofuels, as well as its microbiological and biotechnological potential. Results indicate that only about half of the 180–200 million tonnes of whey produced annually is effectively valorized, underscoring the need for integrated circular-economy approaches. Overall, whey valorization contributes to sustainable food production, environmental protection, and the development of innovative, health-promoting products that align with global strategies for waste reduction and the development of functional foods. Full article

Background/Objectives: Glioblastoma is the most common and aggressive primary brain tumor in adults, with patient prognosis remaining poor. Treatment resistance and tumor recurrence are frequent, primarily due to the high intra- and inter-tumoral heterogeneity and the existence of glioma stem cells. Thus, there is an urgent need for novel and more effective therapeutic strategies. Multitarget small molecules (MSMs) are emerging as a novel therapeutic strategy for the treatment of complex diseases such as cancer. Methods: In the present work, we have generated a novel family of indole-based MSMs with pharmacophoric moieties combining the parent compounds Contilisant and the HDAC inhibitor Tubastatin A. Thus, the MSMs were designed to inhibit monoamine oxidases (MAOs), cholinesterases (ChEs) and histone deacetylases (HDACs), while acting as histamine H3 receptor (H3R) antagonists and sigma 1 receptor (S1R) agonists. We generated four different molecules and evaluated in detail the activity of the two most efficient MSM compounds in vitro and in vivo. Results: These molecules induced potent cytotoxic effects in vitro in patient-derived glioma stem cells and glioblastoma cell lines and significantly impaired tumor growth in vivo. OMIC analyses further revealed that the compounds induce dysregulation of the cell cycle in glioma stem cells. Moreover, in silico analyses indicated that these compounds are theoretically capable of crossing the blood–brain barrier, while exhibiting low toxicity in healthy cells. Conclusions: In conclusion, our findings demonstrate the potential antitumor activity of a novel family of MSMs in preclinical models of glioblastoma. Full article

Low Power Wide Area Networks (LPWAN) have emerged as essential connectivity solutions for the Internet of Things (IoT), addressing requirements for long range, energy efficient communication that traditional wireless technologies cannot meet. With LPWAN connections projected to grow at 26% compound annual growth rate until 2027, understanding real-world performance is crucial for technology selection. This review examines four leading LPWAN technologies—LoRaWAN, Sigfox, Narrowband IoT (NB-IoT), and LTE-M. This review analyzes 20 peer reviewed studies from 2015–2025 reporting real-world deployment metrics across power consumption, range, data rate, scalability, availability, and security. Across these studies, practical performance diverges from vendor specifications. In the cited rural and urban LoRaWAN deployments LoRaWAN achieves 2+ year battery life and 11 km rural range but suffers collision limitations above 1000 devices per gateway. Sigfox demonstrates exceptional range (280 km record) with minimal power consumption but remains constrained by 12 byte payloads and security vulnerabilities. NB-IoT provides robust performance with 96–100% packet delivery ratios at −127 dBm on the tested commercial networks, and supports tens of thousands devices per cell, though mobility increases energy consumption. In the cited trials LTE-M offers highest throughput and sub 200 ms latency but fails beyond −113 dBm where NB-IoT maintains connectivity. NB-IoT emerges optimal for large scale stationary deployments, while LTE-M suits high throughput mobile applications. Full article

Water stress is among the most important abiotic constraints affecting forest ecosystem functioning and regeneration, a phenomenon expected to intensify with climate change. It impacts photosynthesis, growth, and seedling survival, therefore threatening biodiversity and accelerating forest degradation. The use of silicon-based biostimulants has emerged as a way of mitigating the effects of water stress by improving water status and stimulating mechanical and biochemical defense. However, its effectiveness on forest tree species remains poorly explored. This study examines how potassium silicate (PS) alleviates the effects of drought on Canarium madagascariense, with the aim of improving our understanding of the resilience mechanisms of tropical forest species. To do this, an experiment with 135 two-year-old C. madagascariense saplings has been conducted, testing three irrigation levels in combination with the addition of potassium silicate (PS) at concentrations of 5 and 10 mM, via foliar spraying and soil application. Morphometric and physiological parameters were monitored, followed by the biochemical profiling of the induced responses. Linear mixed models were computed to assess the effects of the different factors on the different growth performance, physiological functioning parameters over time, and ANOVA was used for evaluating the punctual data on the biochemical compounds. Drought had a significant impact on the morphological and physiological behaviour of the seedlings. However, the application of PS modified the drought-induced changes, even at a low concentration of 5 mM. Biochemical defenses were also improved further with PS application. Hormone profiling revealed a predominance of auxins, while abscisic acid was lower in the water stress treatments under drought. Therefore, using PS could support the production of robust seedlings that are more tolerant of, and adaptive to, the challenges of climate change, making restoration more efficient. Full article