Search Results (46,333)

Safe drinking water and microbial inactivation from surfaces and devices are among the World Health Organization’s priorities. Plasma-activated water (PAW) inactivates microorganisms mainly by producing radicals (hydroxyl radicals, superoxide, nitrogen oxide, etc.), which form secondary reactive species like nitrates, nitrites, hydrogen peroxide, etc., from the air–liquid interface, where the plasma interacts with the water. A plasma arc device for water treatment with enhanced arc length was constructed at the Andronikashvili Institute of Physics (TSU) and used in the study. PAW’s antibacterial efficacy has been evaluated against Gram-negative E. coli and remarkably stress-resistant Gram-positive B. pumilus. This study identifies reactive oxygen (hydrogen peroxide and superoxide anions) and nitrogen species (total nitrate and nitrite ions) in plasma-activated water, analyzing their potential impact on antioxidant enzyme activity and their relationships with bacterial cell viability. B. pumilus exhibits greater resistance to plasma-activated water as a disinfectant compared to E. coli. Catalase is more effective than superoxide dismutase in protecting cells from external oxidative stress, based on the two antioxidant enzymes studied. Full article

This study investigates the factors associated with niyyah (worshipful intention) of Islamic bankers toward issuing green sukuk (G-sukuk) investment instruments. In particular, it analyses how bankers’ empathy, moral and ethical responsibilities, and self-efficacy are related with environmental awareness, perceived social support, and green tech innovation, respectively. These factors then predicted bankers’ niyyah toward issuing G-sukuk. The present research employed the theory of bounded rational planned behavior as its theoretical foundation. Data were collected from 390 bankers employed in different Islamic banks. Random sampling technique was employed for this cross-sectional study and for analyzing data, this study applied structural equation modeling. Findings indicate that all predictors are statistically significant and positively associated with bankers’ niyyah toward G-sukuk for ensuring sustainable finance. Furthermore, G-sukuk initiatives can help to lower the carbon emissions and other harmful substances, which would improve overall environmental sustainability and ecological contexts related to SDG-13. There is limited empirical evidence available on the G-sukuk perspective in Bangladesh. This study will provide practical insights for the bankers and policymakers. Full article

Despite the enormous amounts of waste textiles produced by the world’s textile industry’s explosive growth, resource utilization rates are still poor. Cotton/polyester blended waste fabrics make up a sizable share, and sorting them precisely is essential to increasing recycling value and promoting the circular economy in the textile industry. Traditional mechanical and human sorting techniques are ineffective and inaccurate; current spectral analysis algorithms mainly concentrate on quantitative composition prediction and are insufficiently capable of differentiating between waste fabrics with comparable content gradients. To address these challenges, this paper proposes an improved 1DCNN model (Dual-1DCNN-Residual-SE) integrated with Near-Infrared (NIR) hyperspectral imaging technology. This model takes raw spectral data and Savitzky-Golay (SG) smoothing data as dual-channel inputs, introducing residual connections to capture subtle spectral differences between similar fabric categories, and employs SE attention mechanisms to adaptively enhance key features. Comparative experiments with four traditional algorithms—KNN, RF, SVM, and PLS—demonstrate that the proposed model achieves a classification accuracy of 95.94%, surpassing the best traditional algorithm SVM (88.12%) by 7.82%. Ablation experiments confirm each enhanced module’s efficacy. This study achieves high-precision classification of cotton/polyester blended waste fabrics, providing technical support for intelligent sorting of industrial waste fabrics. Full article

This pilot EEG study examined the feasibility of a soundscape-based 40 Hz auditory stimulation format by using a soundscape-only condition as a contrast control. We tested whether a nature-based soundscape with an additively layered pure 40 Hz sine component (40 Hz ON; not amplitude-modulated) yields a more pronounced narrowband response centered at 40 Hz than the same soundscape without the 40 Hz layer (40 Hz OFF). Participants completed both conditions in a single-blind, randomized-order, within-participant crossover session with a washout interval. EEG outcomes included 40 Hz power, frequency-domain SNR around 40 Hz, scalp distribution of 40 Hz power, and phase-based connectivity in the gamma range. This study evaluates EEG-level detectability of 40 Hz–centered neural signatures and does not assess cognitive/clinical efficacy or therapeutic benefit. Across metrics, the 40 Hz ON soundscape showed a consistent ON > OFF directionality, including localized electrode-level signals and a temporal-region summary measure under nominal, uncorrected testing, accompanied by a clearer narrowband feature near 40 Hz in spectral profiles. Overall, the observed trends are consistent with the feasibility of embedding an additive 40 Hz layer into a naturalistic soundscape in a manner that yields EEG-quantifiable, 40 Hz centered signatures; however, because this is an exploratory pilot without multiplicity control, all effects should be interpreted as hypothesis-generating and warrant confirmation in larger, preregistered studies with multiplicity-aware inference. Full article

Background/Objectives: Work–family conflict (WFC) is a common stressor for working parents and has been associated with poorer child adjustment. However, the mechanisms linking WFC to young children’s emotional and behavioral difficulties remain insufficiently understood. This study examined whether parental mental health mediates the association between WFC and children’s emotional and behavioral difficulties in early childhood. Methods: This quantitative cross-sectional study was conducted in Portugal with 313 parents of children aged 18–72 months. Parents completed validated self-report measures of WFC, parental stress, depressive symptoms, parental self-efficacy, and children’s emotional and behavioral difficulties. Mediation and moderated mediation analyses were performed, testing children’s age (toddlers vs. preschool-aged) as a moderator. Results: Higher WFC was associated with greater emotional and behavioral difficulties in children (parents reported). This association was fully mediated by parental stress and depressive symptoms, whereas parental self-efficacy did not show a significant mediating effect. The indirect pathways were consistent across children’s age groups. Conclusions: The findings indicate that WFC may affect young children’s adjustment, primarily through its impact on parental psychological distress. Supporting parental mental health and reducing WFC may be key targets for early prevention and intervention. Full article

No US Food and Drug Administration (FDA)-approved prophylaxis is currently available for Acute Radiation Syndrome (ARS), which remains a significant threat to military and civilian populations. In this study, we investigated Vasculotide (VT), a Tie2 receptor agonist mimic, as a novel pre-exposure prophylaxis designed to stabilize the vascular endothelium, one of primary targets of radiation-induced damage. To evaluate its efficacy, female B6D2F1/J mice were exposed to 9.5 Gy total body irradiation (TBI), with VT administered subcutaneously at 12 and 2 h prior to exposure. Assessments included 30-day survival, biomarkers of vascular injury, proinflammatory cytokine/chemokine profiling, and evaluation of hematopoietic (H) and gastrointestinal (GI) recovery. Our findings demonstrate that VT significantly increased 30-day survival in a dose-dependent manner, achieving a 30% survival advantage at the 20 μg/kg dose. Furthermore, VT provided robust protection against radiation-induced vascular activation and injury, effectively alleviating damage to the bone marrow (BM) and GI tract. Taken together, these results identify VT as a promising prophylactic countermeasure for ARS. By targeting the Tie2 pathway to preserve vascular integrity, VT addresses a critical gap in medical countermeasures, offering a viable strategy to enhance survival and accelerate multi-organ recovery in radiological mass-casualty scenarios. Full article

The accurate estimation of the instantaneous UV Index (UVI) is critical for public health, yet it is often attempted using broadband pyranometers (measuring Global Horizontal Irradiance GHI) or photometers (measuring Lux). This approach is known to be unreliable, particularly under the complex radiative conditions induced by clouds. However, the physical mechanisms driving this failure, specifically the changes in the spectral quality of sunlight, are not fully quantified. This study utilizes a high-resolution spectroradiometer and pyranometer at a Mediterranean site (Rende, Italy), analyzing instantaneous UVI, GHI and a set of derived analytical metrics: the Erythemal Efficacy, the UV Spectral Quality Ratio and the Clearness Index. The core metric of the paper is the Erythemal Efficacy, designed to quantify the “spectral quality” or “biological hazard” per unit of total energy. It is defined as the ratio of the instantaneous UV Index to the instantaneous GHI measured by the pyranometer. The analysis confirms a decoupling between instantaneous UVI and broadband GHI, exhibiting a wide, non-functional scatter. The paper shows that this failure is caused by the high variability of the Erythemal Efficacy, which is not a constant. Its variability is shown to be linearly governed by the internal Ultraviolet A to Ultraviolet B (UVA/UVB) spectral ratio. Most critically, the Erythemal Efficacy was found to follow a counter-intuitive trend, increasing significantly as the Clearness Index decreases. The common assumption of clouds as spectrally “grey” attenuators is flawed. Clouds act as selective filters, attenuating the GHI, dominated by Visible to Near-Infrared (VIS/NIR), more severely than the UVI. This increases the relative biological hazard of the light that penetrates thick cloud cover. This study provides a physical explanation for the failure of broadband proxies and demonstrates that instantaneous GHI or Lux-based UVI alerts are fundamentally unreliable, as they fail to capture the critical variability of spectral quality. Full article

Antivenom administration is currently the only therapy for snakebite envenoming. However, in sub-Saharan Africa, inadequate quality control systems have led to deficits in the availability, accessibility, efficacy and safety of regionally available antivenoms, which, in turn, hinder snakebite treatment and management in the region. To address this impediment to snakebite treatment in Kenya, this study aimed to assess the preclinical neutralising potencies of four different antivenoms previously or currently available in Kenya (SAIMR polyvalent, AFRIVEN, PANAF-Premium^TM and Inoserp^TM) against key snakes of medical importance in the region, towards establishing a national antivenom quality control laboratory. Venoms were extracted from the Kenyan “big five” medically important snake species: Naja ashei, Naja pallida, Naja nigricollis, Dendroaspis polylepis and Bitis arietans, and their lethal potencies were determined using a murine median lethal dose (LD₅₀) assay. In vitro immunological assays (ELISAs and immunoblotting) and an established preclinical murine in vivo neutralisation assay (median effective dose [ED₅₀]) were used to assess the immunoglobulin-binding and venom-neutralising efficacies of the test antivenoms. In vitro assays revealed high venom-binding titres of SAIMR polyvalent, AFRIVEN and PANAF-Premium^TM, and reactivity to a wide range of venom proteins across the different snake venoms. Contrastingly, Inoserp^TM antivenom had low binding titres and poor reactivity to the snake venom proteins. These findings were aligned with the in vivo results, where SAIMR polyvalent, AFRIVEN and PANAF-Premium^TM showed potent venom-neutralising efficacies against all the tested snake venoms, while Inoserp^TM had low potency and failed to neutralise the lethal effects of N. ashei, N. pallida and D. polylepis venoms at the manufacture-claimed doses. Based on these robust preclinical results, we conclude that SAIMR polyvalent, AFRIVEN and PANAF-Premium^TM antivenoms offer considerable potential for the treatment of envenoming by diverse medically important snakes in Kenya. The observed deficiencies with the Inoserp^TM product highlight the importance of (i) robust, independent preclinical antivenom efficacy testing and (ii) the value of establishing a quality control laboratory to inform local regulatory and procurement decision making. Full article

Advanced chronic kidney disease (CKD) is associated with impaired humoral immunity, contributing to increased infection-related mortality and suboptimal vaccine responses, as notably observed during the COVID-19 pandemic. CKD is also marked by the accumulation of uremic toxins, but whether they directly influence T and B cell functionality remains unclear. In this translational study, we integrated clinical and biological data from 106 CKD patients with mechanistic insights from in vitro and in vivo murine models to identify the mechanisms underlying CKD-associated defects in humoral responses against T cell-dependent antigens. Contrary to our initial hypothesis, indoxyl sulfate—despite its known ability to activate Aryl hydrocarbon Receptor signaling in monocytes—did not directly impair T–B cell cooperation in coculture assays. Similarly, plasma levels of ten major uremic toxins showed no correlations with vaccine-induced antibody titers in patients. Instead, systemic inflammation emerged as the primary driver of defective humoral immunity. Murine models further confirmed that inflammation, rather than uremia alone, induces lymphopenia, disrupts lymphoid architecture, and ultimately impairs antibody production. These findings indicate that CKD-associated inflammation, rather than a direct effect of uremic toxins on adaptive immune effectors, underlies humoral immune dysfunction in CKD. Targeting inflammation may, therefore, offer a promising strategy to improve vaccine efficacy and reduce infection-related complications in this vulnerable population. Full article

Background: Meniscal injuries are common athletic injuries, and isolated meniscal repair is a critical procedure for restoring knee function. However, rehabilitation protocols after meniscal repair remain controversial. This systematic review aimed to evaluate rehabilitation protocols to determine the best strategies for enhancing recovery following isolated meniscal repair. Objectives: Analyze current rehabilitation protocols following isolated meniscal repair, focusing on the efficacy of approaches in improving functional outcomes and reducing recovery time. This study also aims to identify gaps in the existing literature and provide recommendations for future studies. Data sources: Search was conducted using PubMed, Scopus, and Web of Science databases, covering studies published between May 2015 and May 2024. Inclusion criteria: Studies reporting on isolated meniscal repair with defined postoperative rehabilitation protocols and quantifiable outcome measures. Data extraction: Focused on patient demographics, meniscal tear types, repair techniques, and rehabilitation outcomes. The methodological quality of the included studies was assessed, and narrative synthesis was conducted. Results: The review included 13 studies with significant variability in rehabilitation protocols and outcomes. Early weight-bearing and range of motion exercises have been associated with improved recovery in some studies for stable meniscal tears. However, conservative approaches have better outcomes in patients with complex tears. The use of adjunctive therapies such as blood flow restriction training has demonstrated potential in enhancing muscle preservation and overall recovery. Conclusions: Rehabilitation protocols after meniscal repair surgery should be individualized. Although innovative protocols show promise, further research is needed to standardize rehabilitation approaches and optimize long-term outcomes. Full article

The growing interest in sustainable food systems has spurred research into the valorisation of agro-industrial by-products as sources of bioactive compounds. This review provides a comprehensive overview of the phytochemical composition, bioactivity, biotransformation, and potential nutraceutical applications of by-products from chestnut (Castanea sativa Mill.) and grape (Vitis vinifera L.). Recent studies identify matrices such as chestnut leaves, shells, and burs, as well as grape pomace, skins, seeds, stems, and vine shoots, as rich in phenolic compounds, dietary fibres, vitamins, and minor bioactives, with antioxidant, anti-inflammatory, and antimicrobial properties. Emerging evidence highlights the importance of gastrointestinal digestion and microbial biotransformation in modulating the bioavailability and biological efficacy of phenolic compounds, particularly fibre-bound phenolics. The review further discusses state-of-the-art analytical approaches for chemical characterisation, including chromatographic and spectrophotometric methods, as well as emerging strategies for extraction, encapsulation, and delivery to enhance stability and bioavailability. Finally, the integration of chestnut and grapevine by-products into nutraceuticals, functional foods, and natural preservatives is critically examined from technological, safety, regulatory, and sustainability perspectives. Overall, this synthesis underscores the potential of these underutilised biomass streams as multifunctional raw materials that support waste valorisation, resource efficiency, and the development of next-generation health-promoting ingredients aligned with circular bioeconomy principles. Full article

Background: Chagas disease is a major public health problem, especially in Latin American countries, and benznidazole and nifurtimox are currently the only drugs available for its treatment. However, they present several disadvantages, such as low availability, high toxicity, and limited efficacy, which often result in treatment discontinuation. In recent decades, bioinformatics studies have accelerated the field of drug repurposing, reducing time and costs. In this study, the aim was to identify novel cruzain inhibitors from the analogs of FDA-approved drugs with trypanocidal activity. Methods: A ligand-based virtual screen, along with molecular docking analysis, was carried out, and the selected compounds were evaluated for their trypanocidal activity against trypomastigotes of two endemic Mexican strains and their inhibitory activity on cysteine proteases. Results: A cefsulodin analog (LC₅₀ = 126.18 and 77.50 µM), two flucloxacillin analogs (LC₅₀ = 94.05 and 101.73 µM; 48.74 and 64.49 µM), and one piperacillin analog (LC₅₀ = 48.46 and 83.68 µM) had better trypanocidal activity and selectivity index against the NINOA and INC-5 strains than the reference drugs. Enzymatic evaluation showed that all four compounds inhibited cysteine proteases (IC₅₀ < 840.03 µM). Furthermore, molecular dynamics simulations predicted the stability of the compound–protein complex, while the docking test on human cathepsin L predicted their potential selectivity. Finally, our in silico analysis of ADMET properties showed that all compounds exhibited favorable profiles. Conclusions: These results encourage the development of new and more potent anti-Trypanosoma cruzi agents using FDA-approved drugs as scaffolds. Full article

Addiction poses a major global public health challenge. It is characterized by high prevalence, chronic relapse and limited efficacy of available pharmacotherapies across different substance use disorders. Increasing evidence demonstrates that incretin-based therapies directly modulate metabolic signaling pathways that intercross with central reward and motivational circuits, including hypothalamic-mesolimbic networks and dopaminergic neurotransmission. As a result, agents such as glucagon-like peptide 1 receptor agonists, originally developed for the treatment of type 2 diabetes and obesity, are now being actively investigated for their role in addiction treatment. This narrative review summarizes the current knowledge on the role of incretin-based therapies in the neurobiology of addiction. Evidence from preclinical models and human studies supports the potential therapeutic effect of glucagon-like peptide 1 receptor agonists in the treatment of alcohol use disorder, nicotine dependence, and the administration of other psychoactive substances, including psychostimulants, opioids, and cannabinoids. Preclinical studies consistently demonstrate that glucagon-like peptide 1 receptor agonists reduce substance intake, attenuate reward-related behaviors, and suppress relapse-like responding. So far, human evidence remains limited and is largely derived from observational studies. Preliminary research suggests potential reductions in substance use severity and overdose risk among individuals treated with incretin-based agents. While these findings highlight incretin signaling as a promising therapeutic option in addiction, the current evidence is insufficient to support their routine clinical use in the treatment of substance dependence. Therefore, further research is required to clarify underlying mechanisms and establish clinical efficacy. In particular, well-designed randomized controlled trials are needed to determine safety, tolerance and effectiveness of incretin-based therapies across different substance use disorders. Full article

Background and Objectives: Palmoplantar pustulosis (PPPust) and palmoplantar psoriasis (PPso) are distinct palm/sole dermatoses that have historically shared the abbreviation “PPP”. Though the two—since the advent of advanced biotechnology—are now deemed separate diagnoses, each still falls under the ‘palmoplantar spectrum’. It is important to note that PPso and PPPust are each distinct from generalized pustular psoriasis (GPP), a condition that is outside the scope of our study. We quantified the relative efficacy of biologic and small-molecule monotherapies on the palmoplantar spectrum using Bayesian network meta-analyses (NMAs). Materials and Methods: On 6 November 2025, we searched PubMed, Scopus, ClinicalTrials.gov, and citations (i.e., citation mining) for randomized trials of monotherapy reporting PPP Area and Severity Index (PPPASI) outcomes at 12 or 16 weeks; we secondarily investigated fresh pustule-related outcomes at 4 weeks. We ran Bayesian NMAs with uniform priors; nodes were defined by dose and timepoint. Interventions’ Surface Under the Cumulative Ranking Curve (SUCRA) values were computed; pairwise effects with 95% credible intervals were also estimated. Sensitivity analyses adjusted for diagnosis (pustulosis vs. psoriasis) via network meta-regression. Results: Twenty trials (n = 2030) with 23 active comparators provided data for 10 endpoints (fresh pustules at 4 weeks; PPPASI-50/75 and mean percentage and absolute PPPASI change at 12 and 16 weeks). Conclusions: The NMA indicates efficacy of ixekizumab and brodalumab (IL-17 inhibitors), guselkumab (IL-23 inhibitor), and spesolimab (IL-36 inhibitor) in managing palmoplantar pustulosis. Full article

Laser radiation constitutes a promising technological advancement within the integrated weed management toolbox but is hindered by low energy use efficiency. This study investigated the efficiency of a pulsed blue diode laser for controlling small weed seedlings and seeds under controlled conditions. Dose–response experiments were conducted on three grasses (Poa annua, Echinochloa crus-galli, Digitaria sanguinalis) and three dicotyledonous species (Solanum nigrum, Chenopodium album, Senecio vulgaris). For seedlings, the effects of species, growth stage (cotyledon, 2-leaf), and leaf wetness (dry, wet) were tested. For seeds, burial depth (0 mm, 2 mm) and imbibition status (non-imbibed, imbibed) were examined. Biological efficiency was assessed through plant survival, aboveground dry biomass, leaf area, and seed viability. Laser application caused significant, dose-dependent reductions in biomass accumulation and plant survival, with up to 100% mortality. Seedlings were most sensitive at the cotyledon stage and when foliage was dry, requiring up to 68 and 52% lower energy doses compared to older or wet targets, respectively. Species-specific responses were observed, with dicotyledonous species generally requiring 80 to 99% lower energy doses than grasses. Laser exposure was also effective in reducing the viability of non-imbibed, surface-exposed seeds, requiring up to 64 and 99% lower energy doses than imbibed or buried seeds, respectively. These results confirm that laser efficiency is strongly influenced by species traits, developmental stage, surface moisture, and seed water status. Optimising and tailoring laser parameters to these factors enhances weed control efficacy while maximising energy efficiency, improving the performance and sustainability of laser-based weeding. Full article