Search Results (13,179)

Papillomaviruses (PVs) are double-stranded DNA viruses known to induce a variety of epithelial lesions in dogs, ranging from benign hyperplasia to malignancies. In regions of rich biodiversity such as the Western Amazon, data on the circulation and genetic composition of canine papillomaviruses (CPVs) remain scarce. This study investigated CPV types present in oral and cutaneous papillomatous lesions in domiciled dogs from Acre and Rondônia States, Brazil. Sixty-one dogs with macroscopically consistent lesions were clinically evaluated, and tissue samples were collected for histopathological examination and PCR targeting the L1 gene. Among these, 37% were histologically diagnosed as squamous papillomas or fibropapillomas, and 49.2% (30/61) tested positive for papillomavirus DNA. Sequencing of the L1 gene revealed that most positive samples belonged to CPV1 (Lambdapapillomavirus 2), while one case was identified as CPV8 (Chipapillomavirus 3). Complete genomes of three CPV1 strains were obtained via high-throughput sequencing and showed high identity with CPV1 strains from other Brazilian regions. Phylogenetic analysis confirmed close genetic relationships among isolates across distinct geographic areas. These findings demonstrate the circulation of genetically conserved CPVs in the Amazon and reinforce the value of molecular and histopathological approaches for the accurate diagnosis and surveillance of viral diseases in domestic dogs, especially in ecologically complex regions. Full article

To explore the characteristics of species diversity and phylogenetic diversity, as well as the dominant processes of community construction, in different forest types (deciduous broad-leaved forest, mixed coniferous and broad-leaved forest, and Chinese fir plantation) in subtropical regions, analyze the specific driving patterns of soil nutrients and other environmental factors on the formation of forest diversity in different forest types, and clarify the differences in response to environmental heterogeneity between natural forests and plantation forests. Based on 48 fixed monitoring plots of 50 m × 50 m in Shouchang Forest Farm, Jiande City, Zhejiang Province, woody plants with a diameter at breast height ≥5 cm were investigated. Species diversity indices (Margalef index, Shannon–Wiener index, Simpson index, and Pielou index), phylogenetic structure index (PD), and environmental factors were used to analyze the relationship between diversity characteristics and environmental factors through variance analysis, correlation analysis, and generalized linear models. Phylogenetic structural indices (NRI and NTI) were used, combined with a random zero model, to explore the mechanisms of community construction in different forest types. Research has found that (1) the deciduous broad-leaved forest had the highest species diversity (Margalef index of 4.121 ± 1.425) and phylogenetic diversity (PD index of 21.265 ± 7.796), significantly higher than the mixed coniferous and broad-leaved forest and the Chinese fir plantation (p < 0.05); (2) there is a significant positive correlation between species richness and phylogenetic diversity, with the best fit being AIC = 70.5636 and R² = 0.9419 in broad-leaved forests; however, the contribution of evenness is limited; (3) the specific effects of soil factors on different forest types: available phosphorus (AP) is negatively correlated with the diversity of deciduous broad-leaved forests (p < 0.05), total phosphorus (TP) promotes the diversity of coniferous and broad-leaved mixed forests, while the diversity of Chinese fir plantations is significantly negatively correlated with total nitrogen (TN); (4) the phylogenetic structure of three different forest types shows a divergent pattern in deciduous broad-leaved forests, indicating that competition and exclusion dominate the construction of deciduous broad-leaved forests; the aggregation mode of Chinese fir plantation indicates that environmental filtering dominates the construction of Chinese fir plantation; the mixed coniferous and broad-leaved forest is a transitional model, indicating that the mixed coniferous and broad-leaved forest is influenced by both stochastic processes and ecological niche processes. In different forest types in subtropical regions, the species and phylogenetic diversity of broad-leaved forests is significantly higher than in other forest types. The impact of soil nutrients on the diversity of different forest types varies, and the characteristics of community construction in different forest types are also different. This indicates the importance of protecting the original vegetation and provides a scientific basis for improving the ecological function of artificial forest ecosystems through structural adjustment. The research results have important practical guidance value for sustainable forest management and biodiversity conservation in the region. Full article

Oil spill simulation models are essential for predicting the oil spill behavior and movement in marine environments. In this study, we comprehensively reviewed a large and diverse body of peer-reviewed literature obtained from Scopus and Web of Science. Our initial analysis phase focused on examining trends in scientific publications, utilizing the complete dataset derived after systematic screening and database integration. In the second phase, we applied elements of a systematic review to identify and evaluate the most influential contributions in the scientific field of oil spill simulations. Our analysis revealed a steady and accelerating growth of research activity over the past five decades, with a particularly notable expansion in the last two. The field has also experienced a marked increase in collaborative practices, including a rise in international co-authorship and multi-authored contributions, reflecting a more global and interdisciplinary research landscape. We cataloged the key modeling frameworks that have shaped the field from established systems such as OSCAR, OIL-MAP/SIMAP, and GNOME to emerging hybrid and Lagrangian approaches. Hydrodynamic models were consistently central, often integrated with biogeochemical, wave, atmospheric, and oil-spill-specific modules. Environmental variables such as wind, ocean currents, and temperature were frequently used to drive model behavior. Geographically, research has concentrated on ecologically and economically sensitive coastal and marine regions. We conclude that future progress will rely on the real-time integration of high-resolution environmental data streams, the development of machine-learning-based surrogate models to accelerate computations, and the incorporation of advanced biodegradation and weathering mechanisms supported by experimental data. These advancements are expected to enhance the accuracy, responsiveness, and operational value of oil spill modeling tools, supporting environmental monitoring and emergency response. Full article

Animal protein production represents a complex system of lives transformed into nutrition, with profound ethical and environmental implications. This study provides a quantitative analysis of animal lives required to produce human-consumable protein across major food production systems. Categorizing animal lives based on cognitive complexity and accounting for all lives involved in production, including direct harvests, reproductive animals, and feed species, reveals dramatic variations in protein efficiency. The analysis considers two categories of animal life: complex-cognitive lives (e.g., mammals, birds, cephalopods) and pain-capable lives (e.g., fish, crustaceans). Calculating protein yield per life demonstrates efficiency differences spanning more than five orders of magnitude, from 2 g per complex-cognitive life for baby octopus to 390,000 g per life for bovine dairy systems. Key findings expose disparities between terrestrial and marine protein production. Terrestrial systems involving mammals and birds show higher protein yields and exclusively involve complex-cognitive lives, while marine systems rely predominantly on pain-capable lives across complex food chains. Dairy production emerges as the most efficient system. Aquaculture systems reveal complex dynamics, with farmed carnivorous fish requiring hundreds of feed fish lives to produce protein, compared to omnivorous species that demonstrate improved efficiency. Beyond quantitative analysis, this research provides a framework for understanding the ethical and ecological dimensions of protein production, offering insights for potential systemic innovations. Full article

Arbuscular mycorrhizal fungi (AMF) play a vital role in the restoration of tropical forests by enhancing soil fertility, facilitating plant establishment, and improving ecosystem resilience. This study presents a comprehensive bibliometric analysis of global scientific output on AMF in the context of ecological restoration, based on 3835 publications indexed in the Web of Science and Scopus databases from 2001 to 2024. An average annual growth rate of approximately 9.45% was observed, with contributions from 10,868 authors across 880 journals. The most prominent journals included Mycorrhiza (3.34%), New Phytologist (3.00%), and Applied Soil Ecology (2.79%). Thematically, dominant research areas encompassed soil–plant interactions, phytoremediation, biodiversity, and microbial ecology. Keyword co-occurrence analysis identified “arbuscular mycorrhizal fungi,” “diversity,” “soil,” and “plant growth” as core topics, while emerging topics such as rhizosphere interactions and responses to abiotic stress showed increasing prominence. Despite the expanding body of literature, key knowledge gaps remain, particularly concerning AMF–plant specificity, long-term restoration outcomes, and integration of microbial community dynamics. These findings offer critical insights into the development of AMF research and underscore its strategic importance in tropical forest restoration, providing a foundation for future studies and informing ecosystem management policies. Full article

Mangoes are an important part of Hainan’s tropical characteristic agriculture. In response to the requirements of building an ecological civilization pilot demonstration zone in Hainan, China, green and sustainable development will be the future development trend of the mango planting system. However, the economic benefits and environmental impact during its planting and management process remain unclear. This paper combines emergy, life cycle assessment (LCA), and economic analysis to compare the system sustainability, environmental impact, and economic benefits of the traditional mango cultivation system (TM) in Dongfang City, Hainan Province, and the early-maturing mango cultivation system (EM) in Sanya City. The emergy evaluation results show that the total emergy input of EM (1.37 × 10¹⁶ sej ha⁻¹) was higher than that of TM (1.32 × 10¹⁶ sej ha⁻¹). From the perspective of the emergy index, compared with TM, EM exerted less pressure on the local environment and has better stability and sustainability. This was due to the higher input of renewable resources in EM. The LCA results showed that based on mass as the functional unit, the potential environmental impact of the EM is relatively high, and its total environmental impact index was 18.67–33.19% higher than that of the TM. Fertilizer input and On-Farm emissions were the main factors causing environmental consequences. Choosing alternative fertilizers that have a smaller impact on the environment may effectively reduce the environmental impact of the system. The economic analysis results showed that due to the higher selling price of early-maturing mango, the total profit and cost–benefit ratio of the EM have increased by 55.84% and 36.87%, respectively, compared with the TM. These results indicated that EM in Sanya City can enhance environmental sustainability and boost producers’ annual income, but attention should be paid to the negative environmental impact of excessive fertilizer input. These findings offer insights into optimizing agricultural inputs for Hainan mango production to mitigate multiple environmental impacts while enhancing economic benefits, aiming to provide theoretical support for promoting the sustainable development of the Hainan mango industry. Full article

The water-yield function in the Yellow River Basin (YRB) of China for maintaining the basin’s ecological water balance plays a crucial role. Understanding its spatiotemporal variation and the underlying drivers in the basin is crucial for the management, utilization, and development of water resources. Thus, we used the InVEST model to explore its spatiotemporal dynamics across multiple scales (“basin–county–pixel”). Then, we integrated socio-economic and natural factors to elucidate the driving forces and spatial heterogeneity of water-yield dynamics. Our findings indicated that water-yield trends increased in 71.76% of the YRB, and significant water-yield increases were detected in 13.9% of the basin over the past 40 years. A phase-wise comparison revealed a shift in water yield from a decreasing trend in the first two decades to a significant increasing trend in the last two decades. Hotspot analysis revealed that hotspots of increasing water-yield trends have shifted from the downstream section of the basin toward the southwest, while hotspots of decreasing water-yield trends first concentrated in the basin’s southern part and then disappeared. Both natural and socioeconomic factors have exerted positive and negative impacts on water-yield dynamics. Among them, the dynamics of water yield have been predominantly driven by natural variables. Full article

Complete mitochondrial DNA genome annotation of an ecologically and commercially important fish species Cirrhinus cirrhosus was executed with next-generation sequencing (NGS) for nucleotide and phylogenetic analyses. The findings of this study showed that the Cirrhinus cirrhosus mitochondrial genome contained 16,593 bp, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 tRNA genes, and a D-loop region. The overall base composition was 32% adenine, 25% thiamine, 16% guanine, and 27% cytosine. This mitochondrial DNA exhibits an AT biasness, with 56% AT content in its genome. Significant fluctuations were identified in the AT and GC skew values of the ND6 gene, indicating that the selection and mutation forces acting on this gene might be different from those acting on other genes. The Ka/Ks ratios of most protein-coding genes were less than 1, indicating very strong natural selection pressure. Phylogenetic analysis of Cirrhinus cirrhosus with Cirrhinus mrigala and Bangana tungting suggested a closer evolutionary relationship among these species, which might have shared a more recent common ancestor. It has been also found that the genera Labeo and Cirrhinus are not monophyletic. Full article

Situated in the southern hilly-mountain belt of China’s “Three Zones and Four Belts Strategy”, Gannan region is a critical ecological shelter belt for the Ganjiang River. Decades of intensive mineral extraction and irrational agricultural development have rendered it into an ecologically fragile area. Consequently, multiple restoration initiatives have been implemented in the region over recent decades. However, it remains unclear how relationships among ecosystem services have evolved under these interventions and how future ecosystem management should be optimized based on these changes. Thus, in this study, we simulated and assessed the spatiotemporal dynamics of five key ESs in Gannan region from 1990 to 2020. Through integrated correlation, clustering, and redundancy analyses, we quantified ES interactions, tracked the evolution of ecosystem service bundles (ESBs), and identified their socio-ecological drivers. Despite a 31% decline in water yield, ecological restoration initiatives drove substantial improvements in key regulating services: carbon storage increased by 6.9 × 10¹² gC while soil conservation rose by 4.8 × 10⁸ t. Concurrently, regional habitat quality surged by 45% in mean scores, and food production increased by 2.1 × 10⁵ t. Critically, synergistic relationships between habitat quality, soil retention, and carbon storage were progressively strengthened, whereas trade-offs between food production and habitat quality intensified. Further analysis revealed that four distinct ESBs—the Agricultural Production Bundle (APB), Urban Development Bundle (UDB), Eco-Agriculture Transition Bundle (ETB), and Ecological Protection Bundle (EPB)—were shaped by slope, forest cover ratio, population density, and GDP. Notably, 38% of the ETB transformed into the EPB, with frequent spatial interactions observed between the APB and UDB. These findings underscore that future ecological restoration and conservation efforts should implement coordinated, multi-service management mechanisms. Full article

In chili cultivation, obstacles to continuous cropping significantly compromise crop yield and soil health, whereas crop rotation can enhance the microbial environment of the soil and reduce disease incidence. However, its effects on the diversity of rhizosphere soil microbial communities are not clear. In this study, we analyzed the composition and characteristics of rhizosphere soil microbial communities under chili continuous cropping (CC) and chili–cotton crop rotation (CR) using high-throughput sequencing technology. CR treatment reduced the alpha diversity indices (including Chao1, Observed_species, and Shannon index) of bacterial communities and had less of an effect on fungal community diversity. Principal component analysis (PCA) revealed distinct compositional differences in bacterial and fungal communities between the treatments. Compared with CC, CR treatment has altered the structure of the soil microbial community. In terms of bacterial communities, the relative abundance of Firmicutes increased from 12.89% to 17.97%, while the Proteobacteria increased by 6.8%. At the genus level, CR treatment significantly enriched beneficial genera such as RB41 (8.19%), Lactobacillus (4.56%), and Bacillus (1.50%) (p < 0.05). In contrast, the relative abundances of Alternaria and Fusarium in the fungal community decreased by 6.62% and 5.34%, respectively (p < 0.05). Venn diagrams and linear discriminant effect size analysis (LEfSe) further indicated that CR facilitated the enrichment of beneficial bacteria, such as Bacillus, whereas CC favored enrichment of pathogens, such as Firmicutes. Fusarium solani MG6 and F. oxysporum LG2 are the primary chili root-rot pathogens. Optimal growth occurs at 25 °C, pH 6: after 5 days, MG6 colonies reach 6.42 ± 0.04 cm, and LG2 5.33 ± 0.02 cm, peaking in sporulation (p < 0.05). In addition, there are significant differences in the utilization spectra of carbon and nitrogen sources between the two strains of fungi, suggesting their different ecological adaptability. Integrated analyses revealed that CR enhanced soil health and reduced the root rot incidence by optimizing the structure of soil microbial communities, increasing the proportion of beneficial bacteria, and suppressing pathogens, providing a scientific basis for microbial-based soil management strategies in chili cultivation. Full article

The rapid urbanization process has accelerated the degradation of ecosystem services (ESs) in peri-urban rural areas of the Yangtze River Delta (YRD), leading to increasing landscape ecological risks (LERs). Establishing a scientifically grounded landscape ecological risk assessment (LERA) system and corresponding control strategies is therefore imperative. Using rural areas of Jiangning District, Nanjing as a case study, this research proposes an optimized dual-dimensional coupling assessment framework that integrates ecosystem service value (ESV) and ecological risk probability. The spatiotemporal evolution of LER in 2000, 2010, and 2020 and its key driving factors were further studied by using spatial autocorrelation analysis and geodetector methods. The results show the following: (1) From 2000 to 2020, cultivated land remained dominant, but its proportion decreased by 10.87%, while construction land increased by 26.52%, with minimal changes in other land use types. (2) The total ESV increased by CNY 1.67 × 10⁹, with regulating services accounting for over 82%, among which water bodies contributed the most. (3) LER showed an overall increasing trend, with medium- to highest-risk areas expanding by 55.37%, lowest-risk areas increasing by 10.10%, and lower-risk areas decreasing by 65.48%. (4) Key driving factors include landscape vulnerability, vegetation coverage, and ecological land connectivity, with the influence of distance to road becoming increasingly significant. This study reveals the spatiotemporal evolution characteristics of LER in typical peri-urban villages. Based on the LERA results, combined with terrain features and ecological pressure intensity, the study area was divided into three ecological management zones: ecological conservation, ecological restoration, and ecological enhancement. Corresponding zoning strategies were proposed to guide rural ecological governance and support regional sustainable development. Full article

The genus Sonchus (Asteraceae) comprises 98 species, including 17 predominantly herbaceous taxa native to the Mediterranean region. These plants have long been utilized as traditional wild food sources due to their high nutritional value, as they are rich in vitamins A, C, and K, essential minerals, and bioactive compounds with antioxidant and anti-inflammatory properties. This review aims to provide a comprehensive synthesis of the taxonomy, geographic distribution, phytochemical composition, traditional uses, historical significance, and pharmacological properties of Sonchus species. A systematic literature search was conducted using PubMed, Scopus, Web of Science, and Google Scholar, focusing on studies from 1980 to 2024. Inclusion and exclusion criteria were applied, and methodological quality was assessed using standardized tools. A bibliometric analysis of 440 publications (from 1856 to 2025) reveals evolving research trends, with S. oleraceus, S. arvensis, and S. asper being the most extensively studied species. The review provides detailed taxonomic insights into 17 species and 14 subspecies, emphasizing their ecological adaptations and biogeographical patterns. Additionally, it highlights the cultural and medicinal relevance of Sonchus since antiquity while underscoring the threats posed by environmental degradation and changing dietary habits. Sonchus oleraceus and S. tenerrimus dominate the culinary applications of the genus, likely due to favorable taste, wide accessibility, and longstanding cultural importance. The comprehensive nutritional profile of Sonchus species positions these plants as valuable contributors to dietary diversity and food security. Finally, the study identifies current knowledge gaps and proposes future research directions to support the conservation and sustainable utilization of Sonchus species. Full article

Vegetation dynamics are complexly influenced by multiple factors such as climate, human activities, and topography. In recent years, the frequency, intensity, and diversity of human activities have increased, placing substantial pressure on the growth of vegetation. Arid and semi-arid regions are particularly sensitive to climate change, and climate change and large-scale ecological restoration have led to significant changes in the dynamic of dryland vegetation. However, few studies have explored the nonlinear relationships between these factors and vegetation dynamic. In this study, we integrated trend analysis (using the Mann–Kendall test and Theil–Sen estimation) and machine learning algorithms (XGBoost-SHAP model) based on long time-series remote sensing data from 2001 to 2020 to quantify the nonlinear response patterns and threshold effects of bioclimatic variables, topographic features, soil attributes, and anthropogenic factors on vegetation dynamic. The results revealed the following key findings: (1) The kNDVI in the study area showed an overall significant increasing trend (p < 0.01) during the observation period, of which 26.7% of the area showed a significant increase. (2) The water content index (Bio 23, 19.6%), the change in land use (15.2%), multi-year average precipitation (pre, 15.0%), population density (13.2%), and rainfall seasonality (Bio 15, 10.9%) were the key factors driving the dynamic change of vegetation, with the combined contribution of natural factors amounting to 64.3%. (3) Among the topographic factors, altitude had a more significant effect on vegetation dynamics, with higher altitude regions less likely to experience vegetation greening. Both natural and anthropogenic factors exhibited nonlinear responses and interactive effects, contributing to the observed dynamic trends. This study provides valuable insights into the driving mechanisms behind the condition of vegetation in arid and semi-arid regions of China and, by extension, in other arid regions globally. Full article

The placement of hydraulic elements in existing pool-type fishways to make them more suitable for Cyprinid fish is an issue of increasing interest in fishway research. Hydrodynamic characteristics and fish behavior at the representative pool of the fishway with bottom orifices and notches were assessed at the Dagdelen hydropower plant in the Ceyhan River Basin, Türkiye. Three-dimensional velocity measurements were taken in the pool of the fishway using an Acoustic Doppler velocimeter. The measurements were taken with and without a brush block at two different vertical distances from the bottom, which were below and above the level of bristles tips. A computational fluid dynamics (CFD) analysis was conducted for the studied fishway. The numerical model utilized Large Eddy Simulation (LES) combined with the Darcy–Forchheimer law, wherein brush blocks were represented as homogenous porous media. Our results revealed that the relative submergence of bristles in the brush block plays a very important role in velocity and Reynolds shear stress (RSS) distributions. After the placement of the submerged brush block, flow velocity and the lateral RSS component were reduced, and a resting area was created behind the brush block below the bristles’ tips. Fish movements in the pool were recorded by underwater cameras under real-time operation conditions. The heatmap analysis, which is a 2-dimensional fish spatial presence visualization technique for a specific time period, showed that Capoeta damascina avoided the areas with high turbulent fluctuations during the tests, and 61.5% of the fish presence intensity was found to be in the low Reynolds shear regions in the pool. This provides a clear case for the real-world ecological benefits of retrofitting existing pool-weir fishways with such flexible hydraulic elements. Full article

Karst groundwater systems are critical to water supply and ecological sustainability in northern China, yet their heterogeneity poses challenges for flow characterization. The Yuquan Mountain (YM) Spring, historically a major karst spring in western Beijing, has experienced persistent drying, raising concerns about its recharge and flow mechanisms. This study integrates published isotope data, spatial distributions of Na⁺ and Cl⁻ as hydrochemical tracers, groundwater age estimates, and geophysical survey results to assess the recharge sources and flow pathways within the YM Spring catchment area. The analysis identifies two major recharge zones: the Tanzhesi area, primarily recharged by direct infiltration of precipitation through exposed carbonate rocks, and the Junzhuang area, which receives mixed recharge from rainfall and Yongding River seepage. Three potential flow pathways are proposed, including shallow flow along faults and strata, and a deeper, speculative route through the Jiulongshan-Xiangyu syncline. The synthesis of multiple lines of evidence leads to a refined conceptual model that illustrates how geological structures govern recharge, flow, and discharge processes in this karst system. These findings not only enhance the understanding of subsurface hydrodynamics in complex geological settings but also provide a scientific basis for future spring restoration planning and groundwater management strategies in the regions. Full article