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39 pages, 1923 KB  
Systematic Review
Intermittent Fasting and Healthy Aging in Older Adults: A Systematic Review of Cardiometabolic, Mental Health and Cognitive Outcomes with a Network Meta-Analysis of Anthropometric Measures
by Sergio Couto-Alfonso, María Carmen Cenit, Cristina María Sanz-Pérez and Isabel Iguacel
Nutrients 2026, 18(9), 1450; https://doi.org/10.3390/nu18091450 (registering DOI) - 30 Apr 2026
Abstract
Background/Objective: Intermittent fasting (IF) shows promise for metabolic and mental health benefits, but evidence in older adults remains limited. This study systematically evaluated the safety and effectiveness of IF in adults aged ≥60 years, comparing different protocols using network meta-analysis. Methods: [...] Read more.
Background/Objective: Intermittent fasting (IF) shows promise for metabolic and mental health benefits, but evidence in older adults remains limited. This study systematically evaluated the safety and effectiveness of IF in adults aged ≥60 years, comparing different protocols using network meta-analysis. Methods: Systematic review and network meta-analysis following Cochrane and PRISMA guidelines were conducted, producing a literature search until June 2025 across PubMed, Scopus, and ScienceDirect databases, with inclusion criteria comprising randomized controlled trials, clinical trials, and observational studies evaluating IF in adults ≥60 years. Network meta-analysis compared time-restricted eating (TRE), IF 5:2 method, Islamic Sunnah fasting (ISF), Healthy Living Diet and usual diet. The NMA was conducted exclusively using randomized controlled trials (RCTs; n = 7); pre–post trials and observational studies were included solely in the narrative systematic review component and did not contribute to any pooled NMA estimates. Observational data contributed exclusively to the narrative synthesis. Results: Thirty-one studies were included; seven RCTs were eligible for network meta-analysis. ISF and TRE 16:8 were most effective for weight (ISF: −2.36 kg; TRE 16:8: −1.92 kg) and BMI reduction (−0.81 and −1.01 kg/m2) without lean mass loss. Findings on cardiometabolic parameters, mental health, and cognitive function are based on the narrative synthesis of individual studies. Long-term structured IF was associated with improvements in standardized cognitive performance assessed via validated instruments. However, very restrictive eating windows (≤10 h) and prolonged fasting (>12.38 h) were associated with adverse outcomes, including lower cognitive scores and 58% increased cardiovascular mortality. Conclusions: TRE 16:8 and ISF showed the strongest comparative evidence for weight reduction in the RCT-based NMA, with acceptable short-term safety profiles in the included trials. In the narrative review, these protocols were associated with clinically meaningful improvements in body weight, metabolic markers, and blood pressure while generally preserving lean muscle mass in older adults. The cardiovascular mortality risk associated with very restrictive eating windows may emphasize the importance of moderate fasting approaches in this vulnerable population. Further long-term research is needed to confirm optimal protocols and identify at-risk subgroups. Full article
17 pages, 554 KB  
Article
Chrononutrition and Physical Fitness in Schoolgirls Aged 10–14 Years: Associations with Obesity Risk
by Hessa A. Alhabib, Shaea A. Alkahtani and Maha H. Alhussain
Nutrients 2026, 18(9), 1441; https://doi.org/10.3390/nu18091441 - 30 Apr 2026
Abstract
Chrononutrition, which emphasizes the timing, frequency, and regularity of eating in alignment with circadian rhythms, has emerged as an important yet understudied determinant of obesity, particularly in children and adolescents. We aimed to compare chrononutrition and physical fitness between elementary and intermediate schoolgirls [...] Read more.
Chrononutrition, which emphasizes the timing, frequency, and regularity of eating in alignment with circadian rhythms, has emerged as an important yet understudied determinant of obesity, particularly in children and adolescents. We aimed to compare chrononutrition and physical fitness between elementary and intermediate schoolgirls and to examine their associations with obesity. Methods: This cross-sectional study included 457 schoolgirls aged 10–14 years from elementary and intermediate schools. Chrononutrition behaviors were evaluated. Anthropometric measurements and physical fitness, including handgrip strength, standing long jump, and 20 m shuttle run, were assessed. Fasting blood glucose and lipid profile were determined using capillary blood samples. Results: Compared with intermediate students, elementary school students demonstrated more favorable meal-related behaviors, longer overnight fasting durations, and better dietary intake (p < 0.05), along with higher VO2 max and higher standing long jump performance. Conversely, intermediate students exhibited greater absolute handgrip strength. A higher number of meals/day (aOR = 0.68, p = 0.039) and a longer interval between the last meal and bedtime (aOR = 0.78, p = 0.013) were inversely associated with obesity. Furthermore, higher HGS/BMI was associated with lower odds of obesity (aOR = 0.01, p < 0.001), while HDL cholesterol was inversely associated with obesity (aOR = 0.91, p < 0.001). Conclusions: Chrononutrition behaviors and physical fitness varied across school stages and were associated with obesity among school-aged girls. Higher meal frequency, a longer interval between the last meal and bedtime, and greater handgrip strength relative to body mass index (HGS/BMI) were associated with lower odds of obesity. Non-obese students also demonstrated higher VO2 max. These findings suggest that chrononutrition behaviors and physical fitness may contribute to obesity prevention, underscoring the importance of early nutrition and physical activity interventions. Full article
(This article belongs to the Section Pediatric Nutrition)
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18 pages, 6793 KB  
Article
Evaluation of Grapevine Germplasm Resources Based on Phenotypic Traits and SSR Markers
by Huihui Tao, Qian Chen, Guoquan Li, Siyu Wang, Meng Zhang, Weiming Xiao and Chao Xu
Agronomy 2026, 16(9), 911; https://doi.org/10.3390/agronomy16090911 - 30 Apr 2026
Abstract
To clarify the genetic background and biological characteristics of grape germplasm resources and provide theoretical support for germplasm innovation and new-variety breeding, we conducted systematic morphological identification and SSR molecular-marker analysis on 38 core grape germplasms (29 fresh-eating cultivars, 1 local cultivar, and [...] Read more.
To clarify the genetic background and biological characteristics of grape germplasm resources and provide theoretical support for germplasm innovation and new-variety breeding, we conducted systematic morphological identification and SSR molecular-marker analysis on 38 core grape germplasms (29 fresh-eating cultivars, 1 local cultivar, and 8 wild germplasms) from the National Southeast Mountainous Crop Germplasm Repository (Jiangxi·Yichun) and other regions. For morphological identification, 14 quantitative traits and 5 descriptive traits of leaves, floral organs and fruits were determined in strict accordance with the NY/T 2932-2016 Descriptors for Grape Germplasm Resources. For SSR molecular-marker analysis, eight pairs of internationally universal core primers were used for PCR amplification and fluorescence detection referring to the NY/T 3640-2020 Identification of Grape Cultivars Using SSR Markers, and genetic diversity analysis was conducted on 11 local and wild grape germplasms. The results revealed abundant phenotypic diversity among the tested germplasms: the functional leaves of cultivars were predominantly pentagonal and cuneate, while those of wild germplasms were mostly reniform and cordate, with 3–5 lobes for most germplasms; all germplasms were hermaphroditic, except for two wild accessions with unisexual flowers. Significant variations were observed in fruit traits, with the coefficient of variation (CV) of cluster weight and berry weight reaching 67.64% and 50.53%, respectively. The genetic plasticity of weight-related traits was much higher than that of shape- and length-related traits, and the average Shannon–Wiener index (H′) of 19 morphological traits was 3.47, indicating a high level of overall phenotypic diversity. SSR analysis showed that the eight primer pairs amplified a total of 42 genotypes (5.25 per primer pair on average). The population had a mean observed number of alleles (Na) of 5.28, a mean effective number of alleles (Ne) of 7.25, and a mean polymorphism information content (PIC) of 0.74, demonstrating rich genetic diversity and high polymorphism of the tested loci. Cluster analysis divided the 11 local germplasms into four groups, which clearly reflected the genetic relationships among them, and genetic admixture was found in some germplasms due to unclear introduction traceability. In this study, fresh-eating grape cultivars suitable for the climatic conditions of Jiangxi Province were screened, the utilization value of local germplasm resources was clarified, and a two-dimensional evaluation system based on phenotypic traits and SSR molecular markers was constructed. The findings provide basic data and a scientific basis for the precise evaluation, elite gene mining, and new-variety breeding of grape germplasm resources in Jiangxi Province. Full article
12 pages, 311 KB  
Article
Listening to the Body: Interoceptive Awareness and Eating Disorder Vulnerability in Adolescents with Inflammatory Bowel Diseases
by Anna Riva, Gabriele Arienti, Simona Di Guardo, Eleonora Brasola, Giovanna Zuin, Laura Spini, Naire Sansotta, Andrea Eugenio Cavanna and Renata Nacinovich
Children 2026, 13(5), 626; https://doi.org/10.3390/children13050626 - 30 Apr 2026
Abstract
Background/Objectives: Inflammatory bowel diseases (IBDs) are relapsing–remitting gastrointestinal disorders often emerging in adolescence and frequently associated with psychiatric co-morbidities, including eating disorders (EDs). Deficiency in interoception—awareness of internal bodily sensations—is a transdiagnostic feature in EDs, with emerging evidence suggesting its relevance also [...] Read more.
Background/Objectives: Inflammatory bowel diseases (IBDs) are relapsing–remitting gastrointestinal disorders often emerging in adolescence and frequently associated with psychiatric co-morbidities, including eating disorders (EDs). Deficiency in interoception—awareness of internal bodily sensations—is a transdiagnostic feature in EDs, with emerging evidence suggesting its relevance also in IBDs. This study aimed to assess interoceptive abilities in adolescents with IBDs compared to healthy adolescents. Methods: A total of 76 patients with IBDs and 90 healthy controls were enrolled in the study. All participants completed a comprehensive psychometric assessment, including measures of interoceptive sensibility (MAIA-2) and eating-related symptomatology (EDI-3). Results: Up to one in six (15.8%) patients with IBDs were found to be at high risk of developing EDs (EDI-3 Eating Disorder Risk Composite scale >70th percentile). Mean MAIA-2 scores were largely comparable, with the exception of the MAIA-2 Trusting subscale, which assesses whether the experience of one’s body is rated as safe and trustworthy. Specifically, patients with IBDs at high risk of developing EDs reported lower scores than both healthy controls and patients with IBDs at low risk of developing EDs, with a statistically significant difference emerging in the comparison with the latter group (p = 0.044). Conclusions: Adolescents with IBDs who report an elevated risk of developing eating disorders have a psychological profile characterised by increased disordered eating symptomatology, accompanied by selective impairment in interoceptive trust, as evidenced by reduced trust in internal bodily signal. These findings highlight the clinical relevance of thorough clinical assessment and early psychological intervention in this vulnerable population. Full article
24 pages, 943 KB  
Article
Understanding the Tripartite Relationship Between Dietary Practices, Psychological Well-Being, and Disease Experience in Greek Patients with IBD: A Mixed-Methods Exploration
by Dimitra Eleftheria Strongylou, Vaios Svolos, Athanasia Vlachou, Elli Zoupa, Vasiliki-Rafaela Vakouftsi, Anastasia Ntanou, Konstantinos Argyriou, Andreas Kapsoritakis, Fotini Bonoti and Odysseas Androutsos
Nutrients 2026, 18(9), 1439; https://doi.org/10.3390/nu18091439 - 30 Apr 2026
Abstract
Background/Objectives: Diet and mental health constitute two significant modifiable factors affecting Inflammatory Bowel Disease (IBD). The present exploratory study explores potential interrelationships between mental health and eating patterns in IBD patients in Greece. Methods: A mixed-methods approach was followed. Two hundred [...] Read more.
Background/Objectives: Diet and mental health constitute two significant modifiable factors affecting Inflammatory Bowel Disease (IBD). The present exploratory study explores potential interrelationships between mental health and eating patterns in IBD patients in Greece. Methods: A mixed-methods approach was followed. Two hundred and eighty-three individuals living with IBD in Greece (n = 110 UC, n = 173 CD) participated in an online questionnaire survey examining demographic characteristics, anxiety, depression, and dietary attitudes. Fourteen semi-structured interviews explored the lived experiences of diet, mental health, and disease among IBD patients. Results: Quantitative study revealed that 45.77% of patients scored above the clinical cutoff for anxiety (GAD-7 ≥ 10) and 48.37% for depression (PHQ-9 ≥ 10). Patients with UC exhibited a significantly higher prevalence of moderate to severe anxiety (54.5% vs 37.0%, p = 0.004) and depression (54.5% vs 42.2%, p = 0.042) compared to CD. Disordered eating attitudes were present in 27.22% of the total sample, with no significant differences between diagnostic groups (p = 0.985). Thematic analysis revealed three overarching themes, namely (a) ‘life in two phases: IBD impact on health’, (b) ‘mental health and diet interplay—their perceived impact on IBD’ and (c) ‘coping strategies for managing IBD’. Conclusions: Our findings highlight the dynamic interplay among diet, mental health, and IBD experience. The study underscores the importance of developing holistic biopsychosocial interventions integrating medical, dietary, and psychological components for IBD management. Full article
(This article belongs to the Special Issue Diet in the Pathogenesis and Management of Inflammatory Bowel Disease)
20 pages, 1273 KB  
Article
Sensory and Nutritional Quality of First and Second Cuts of Wild Rocket Leaves (Diplotaxis tenuifolia) at the Beginning and the End of Shelf-Life
by Antonio Raffo, Irene Baiamonte, Pasquale Buonocore, Francesca Masciola, Valentina Melini, Elisabetta Moneta, Nicoletta Nardo, Marina Peparaio, Stefania Ruggeri, Eleonora Saggia Civitelli and Pasquale Tripodi
Agriculture 2026, 16(9), 985; https://doi.org/10.3390/agriculture16090985 - 30 Apr 2026
Abstract
The widespread practice of multiple harvests is known to significantly affect the quality of rocket leaves. Thus, ready-to-eat wild rocket (Diplotaxis tenuifolia L.) was produced under commercial conditions using leaves from five different varieties, harvested at both the first and the second [...] Read more.
The widespread practice of multiple harvests is known to significantly affect the quality of rocket leaves. Thus, ready-to-eat wild rocket (Diplotaxis tenuifolia L.) was produced under commercial conditions using leaves from five different varieties, harvested at both the first and the second cut, with a 21-day interval between cuts. Both the sensory and nutritional quality of rocket leaves were evaluated one day after packaging (beginning of shelf-life) and after an additional six days of storage at 6 °C (end of shelf-life). At the beginning of the shelf-life, first-cut leaves generally exhibited a more intense flavor, corresponding to a higher level of isothiocyanates, compared to second-cut leaves. At the end of shelf-life, first-cut leaves showed a greater susceptibility to off-flavor development, corresponding to the release of higher levels of sulfur compounds within the packages. First-cut leaves showed higher content of ascorbic acid and folate with respect of second-cut leaves, both at the beginning and the end of the shelf-life. The commonly held assumption that successive cuts yield leaves with more intense flavor compared to the first cut does not appear to be confirmed. It is likely that the differences in quality observed between leaves from the first and second cuts are not attributable solely to the cutting process or to the sequence of multiple harvest per se, but rather to significant variations in environmental conditions, particularly temperature and light, during the leaf growth period between the two harvests. Full article
(This article belongs to the Topic Nutritional and Phytochemical Composition of Plants)
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12 pages, 660 KB  
Article
Toward Precision Obesity Pharmacotherapy: Using the Eating Behavior Phenotype Scale (EFCA) in Real-World Clinical Practice
by Ronaldo José Pineda-Wieselberg, Andressa Heimbecher Soares, Thiago Fraga Napoli, Nilza Maria Scalissi and João Eduardo Nunes Salles
Nutrients 2026, 18(9), 1419; https://doi.org/10.3390/nu18091419 - 30 Apr 2026
Abstract
Background: Obesity is a heterogeneous chronic disease in which eating behavior phenotypes may influence treatment response. Yet, anti-obesity medication (AOM) selection is still largely guided by anthropometric and metabolic parameters, with limited use of behavioral phenotyping in routine practice. We evaluated whether multidimensional [...] Read more.
Background: Obesity is a heterogeneous chronic disease in which eating behavior phenotypes may influence treatment response. Yet, anti-obesity medication (AOM) selection is still largely guided by anthropometric and metabolic parameters, with limited use of behavioral phenotyping in routine practice. We evaluated whether multidimensional eating behavior changes, measured by the Brazilian Eating Behavior Phenotype Scale (Escala de Fenótipos do Comportamento Alimentar, EFCA), differ across commonly used AOMs in a real-world cohort. Methods: We conducted a retrospective, observational, real-world study in obesity outpatient care settings in São Paulo, Brazil. Adults with obesity (18–65 years) treated with a single principal AOM for 6 months and paired baseline/6-month follow-up EFCA and anthropometric data were included. Analyses focused on early responders (≥5% total body weight loss at 3 months). Five AOM groups available in Brazil were analyzed: semaglutide (oral or subcutaneous), naltrexone/bupropion, sibutramine, topiramate, and tirzepatide. Outcomes included percent weight loss, EFCA total score, and five EFCA subscales (hedonic, emotional, compulsive, hyperphagic, disorganized). Within-medication behavioral changes were assessed using paired tests and standardized effect sizes (Cohen’s dz, 95% CI), summarized in heatmap form. Results: The analytical cohort comprised 66 early responders with paired EFCA assessments at baseline and 6 months. EFCA profiling revealed distinct behavioral response fingerprints across AOMs. Effect size mapping showed predominantly large behavioral effects (many dz ≥ 0.8) in hedonic, emotional, hyperphagic, and compulsive domains. Strongest signals included emotional eating reductions with naltrexone/bupropion (dz 2.04), tirzepatide (dz 1.77), semaglutide (dz 1.52), and topiramate (dz 1.54); hedonic reductions with tirzepatide (dz 2.06), semaglutide (dz 1.55), and naltrexone/bupropion (dz 1.52); hyperphagic reductions with tirzepatide (dz 1.50) and semaglutide (dz 1.34); and compulsive reductions with topiramate (dz 1.41) and consistent effects across tirzepatide, semaglutide, and sibutramine (≈dz 0.95–0.96). Disorganized eating showed heterogeneous/attenuated responsiveness, from near-null with tirzepatide (dz 0.03) to large but imprecise effects in smaller groups (e.g., topiramate dz 1.24, wide CI). Conclusions: In this responder-enriched real-world cohort, AOMs showed distinct and reproducible EFCA behavioral signatures, supporting a clinically actionable phenotype-informed framework to prioritize, sequence, and monitor obesity pharmacotherapy beyond nonspecific weight reduction, while highlighting disorganization as a potential target for adjunctive behavioral strategies. Full article
(This article belongs to the Special Issue Dietary Patterns and Data Analysis Methods)
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34 pages, 847 KB  
Article
Dietary and Oral Hygiene Behaviors Associated with Prevalent Caries Status in School-Aged Children of Northern Italy
by Virginia Troiani, Edoardo Ratti, Daniel Gonnella, Maria Cristina Panzeri, Paola Palestini and Emanuela Cazzaniga
Nutrients 2026, 18(9), 1416; https://doi.org/10.3390/nu18091416 - 29 Apr 2026
Abstract
Background/Objectives: Unhealthy dietary behaviors and suboptimal oral hygiene practices remain common among Italian children, potentially affecting both nutritional and oral health. Dental caries, a preventable yet highly prevalent condition in pediatric populations, has a multifactorial etiology in which lifestyle factors play a key [...] Read more.
Background/Objectives: Unhealthy dietary behaviors and suboptimal oral hygiene practices remain common among Italian children, potentially affecting both nutritional and oral health. Dental caries, a preventable yet highly prevalent condition in pediatric populations, has a multifactorial etiology in which lifestyle factors play a key role. This study aimed to assess the prevalence of dental caries, dietary habits, and oral hygiene behaviors in school-aged children in Lombardy, and to identify factors associated with prevalent caries status. Methods: A cross-sectional study was conducted on 307 schoolchildren aged 9–10 years from ten schools in Northern Italy. Oral health status was evaluated through the plaque index and the DMFT/dmft index during school-based dental examinations. Dietary habits, lifestyle, and oral hygiene practices were collected through structured questionnaires. A mixed-effects logistic regression model was developed to explore potential associations between variables and prevalent caries status. Results: The dietary patterns, weight status, oral hygiene behaviors, and oral health conditions were generally consistent with the national data. Higher plaque index, skipping breakfast, consuming mid-morning snacks, and parental reports of previous caries experiences were retained in the final model. Internal validation suggested reasonable discriminatory ability overall, whereas calibration shows heterogeneity across schools. Conclusions: The findings highlight suboptimal dietary and oral hygiene behaviors among Lombardy schoolchildren and confirm their association with dental caries. Lifestyle-related factors, particularly oral hygiene practices and eating patterns, showed a relevant association with prevalent caries status in the analyzed sample. These results underscore the need for targeted preventive strategies integrating nutritional education and oral health promotion in pediatric populations. Full article
21 pages, 546 KB  
Article
Social Norms Around Diet and Body Image: Evidence from Urban and Rural Vulnerable Groups in Colombia and Mexico
by Ana Cecilia Fernández-Gaxiola, Paula Veliz, Maaike Arts, Rowena Merritt, Ana María Narvaez, Anabelle Bonvecchio Arenas and Cássia Ayres
Behav. Sci. 2026, 16(5), 675; https://doi.org/10.3390/bs16050675 - 29 Apr 2026
Abstract
In Latin America, the double burden of malnutrition is the region’s single most important public health concern for the incoming decade. Latin America’s burden of disease has distinct features in comparison to high-income countries: nearly 20 percent of NCDs are diagnosed in people [...] Read more.
In Latin America, the double burden of malnutrition is the region’s single most important public health concern for the incoming decade. Latin America’s burden of disease has distinct features in comparison to high-income countries: nearly 20 percent of NCDs are diagnosed in people under 60 years of age in Latin America, whereas only about 13 percent of people under 60 years of age in North America and Europe are diagnosed with these diseases. We aimed to better understand decision-making processes, preferences, and norms around food choices to provide input for future programming and policy suggestions at national and regional levels. We included key informant interviews and focus group discussions with parents and adolescents from urban and rural communities in three regions in Colombia and in Mexico. Results showed that food choices considered to be affordable, acceptable, accessible, and aspirational are driven by environmental and social factors that influence individual cognitive decisions. Across the study groups, cognitive biases influenced food decision-making in relation to eating out, natural, homemade, and “moderation”. At the sociological level, conversations, and social influences at home and in communities were strong indicators of dietary practices, health beliefs, and body size attitudes. Full article
17 pages, 859 KB  
Article
Trajectories of Eating Behavior and Health-Related Quality of Life During the First Year After Metabolic Bariatric Surgery: A Longitudinal Study
by Shu Fen Wu, Hong Yi Tung, Yu Rong Hsu, Shih Ting Lo and Tien Chou Soong
Healthcare 2026, 14(9), 1198; https://doi.org/10.3390/healthcare14091198 - 29 Apr 2026
Abstract
Background: Metabolic bariatric surgery (MBS) yields significant but heterogeneous recovery patterns. The longitudinal interplay between evolving eating behaviors and health-related quality of life (HRQoL) remains insufficiently characterized. Objectives: To identify trajectories of eating behavior and HRQoL during the first postoperative year and examine [...] Read more.
Background: Metabolic bariatric surgery (MBS) yields significant but heterogeneous recovery patterns. The longitudinal interplay between evolving eating behaviors and health-related quality of life (HRQoL) remains insufficiently characterized. Objectives: To identify trajectories of eating behavior and HRQoL during the first postoperative year and examine their associations with 12-month outcomes. Methods: A total of 244 patients from two hospitals in Taiwan were followed for 12 months. Dutch Eating Behavior Questionnaire, and Impact of Weight on Quality of Life-Lite were assessed. Group-based trajectory modeling (GBTM) identified latent subgroups, and multiple regression analyzed associations with 12-month HRQoL, adjusting for clinical covariates. Results: GBTM identified two distinct trajectories for restrained, emotional, and external eating. For HRQoL, three trajectories emerged: high-start stable (45–50%), moderate-decline (30–35%), and low-start improving (~20%). In the regression model (R2 = 0.37, p < 0.001), eating behavior trajectories were not independently associated with total HRQoL at 12 months after adjusting for covariates, including baseline BMI and comorbidities. Specifically, restrained eating (β = −1.42, p = 0.502), emotional eating (β = −10.33, p = 0.110), and external eating (β = −5.33, p = 0.160) trajectories did not significantly predict global HRQoL scores. Conclusions: Postoperative adaptation is characterized by substantial heterogeneity, with a significant subgroup experiencing HRQoL decline despite surgery. While eating behavior trajectories align with domain-specific psychosocial trends, early postoperative clinical factors appear to exert a more dominant influence on total HRQoL during the first year. These findings suggest that multidisciplinary support should target specific vulnerable trajectories to optimize long-term outcomes. Full article
(This article belongs to the Section Clinical Care)
18 pages, 645 KB  
Article
The Psychological Dimensions of Dieting: A Two-Phase Study on Body Appreciation, Nutritional Awareness and Mental Well-Being
by Paula Sophia Cozma, Lóránd Dénes and Zsuzsánna Simon-Szabó
Nutrients 2026, 18(9), 1405; https://doi.org/10.3390/nu18091405 - 29 Apr 2026
Abstract
Background/Objectives: Dieting is a widespread behavior that is associated with psychological distress, body dissatisfaction, and eating disorders. Recent research suggests that a body-positive attitude and mindful approach to eating may influence individuals’ experiences with dieting; however, their combined role has been insufficiently explored. [...] Read more.
Background/Objectives: Dieting is a widespread behavior that is associated with psychological distress, body dissatisfaction, and eating disorders. Recent research suggests that a body-positive attitude and mindful approach to eating may influence individuals’ experiences with dieting; however, their combined role has been insufficiently explored. Methods: A two-phase study was conducted among voluntary adults using online data collection. In Phase 1, a cross-sectional survey was completed by 180 participants (71.7% women), assessing dieting behavior, body appreciation, nutritional awareness, psychological distress, well-being, and eating disorders. Correlation analyses, group comparisons, and regression models were performed. In Phase 2, 90 participants entered the pilot and received a brief psychoeducational digital material promoting mindful eating and positive body image. The follow-up assessment was completed by 59, after one month of engagement. Results: Body appreciation and nutritional awareness were positively associated with mental well-being and inversely related to psychological distress (p < 0.001 for all) and to eating disorder screening scores (p < 0.001 and p = 0.046, respectively). More frequent dieting was associated with lower body appreciation (p < 0.001). According to the observed pattern of correlations, body appreciation may play a role in the relationship between dieting and psychological distress. In the intervention phase, greater engagement with the psychoeducational material was associated with higher reported levels of nutritional awareness (p = 0.003) and greater perceived body awareness (p = 0.026) at follow-up; however, due to the exploratory design, findings are preliminary. Conclusions: The results suggest that dieting, as a behavior, may be embedded in broader psychological processes that include body-related attitudes and nutritional awareness. Taking these factors into account may have potential implications for preventive measures aimed at promoting healthier dietary habits, a more positive relationship with one’s body, and mental well-being. Full article
(This article belongs to the Special Issue The Impact of Eating Disorders and Emotional Eating on Health)
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17 pages, 686 KB  
Article
Liquid Chromatography coupled with Mass Spectrometry as an Analytical Strategy to Assess the Occurrence of Potentially Toxic Cyanogenic Glycosides in Edible Microgreens
by Mariachiara Bianco, Ilario Losito, Beniamino Leoni, Onofrio Davide Palmitessa, Massimiliano Renna, Pietro Santamaria, Cosima Damiana Calvano and Tommaso R. I. Cataldi
Plants 2026, 15(9), 1358; https://doi.org/10.3390/plants15091358 - 29 Apr 2026
Abstract
Microgreens are increasingly promoted as sustainable, nutrient-dense foods, yet their content of potentially harmful specialized metabolites remains poorly explored. Here, we developed and applied a reversed-phase liquid chromatography–electrospray ionization high-resolution mass spectrometry (RPLC-ESI-HRMS) method for the detection of cyanogenic glycosides (CNGs) in edible [...] Read more.
Microgreens are increasingly promoted as sustainable, nutrient-dense foods, yet their content of potentially harmful specialized metabolites remains poorly explored. Here, we developed and applied a reversed-phase liquid chromatography–electrospray ionization high-resolution mass spectrometry (RPLC-ESI-HRMS) method for the detection of cyanogenic glycosides (CNGs) in edible microgreens. Method optimization, performed using dhurrin and lotaustralin as model standards, showed that positive ion detection of sodium adducts provided the most informative and selective HRMS/MS response, with diagnostic fragmentation patterns suitable for CNG recognition in complex matrices. Quantitative validation for lotaustralin showed excellent linearity (R2 = 0.998), low detection/quantification limits (LOD 0.16 mg/L; LOQ 0.53 mg/L), good extraction recovery, and a negligible matrix effect. Application of the method revealed a clear species-dependent profile. No detectable CNGs were found in broccoli raab and kale microgreens, supporting their safety as ready-to-eat products in this respect. In contrast, flax microgreens contained four CNGs: linamarin, lotaustralin, linustatin, and neolinustatin. Monoglycosylated species predominated, with lotaustralin quantified at 5.5 ± 0.6 mg/g dry weight and linamarin estimated at even higher levels. Diglycosylated CNGs were present at much lower concentrations and displayed multiple chromatographic peaks, consistent with the occurrence of structurally related isomeric forms. These quantitative results are specific to the flax microgreen samples analyzed here, obtained by pooling the lyophilized material obtained from several plants; thus, they do not account for biological variability among individual plants. Based on the measured CNG levels, flax microgreens showed a non-negligible cyanogenic potential. Assuming 1, 10 and 25% conversion to hydrogen cyanide, the estimated release would be, respectively, about 3, 33 and 81 mg HCN/kg of fresh flax microgreens, values lower than the current EU limit (150 mg HCN/kg of edible product) for flaxseed intended for direct consumption but comparable to values reported for other foods. These findings highlight the need to complement the nutritional evaluation of novel microgreens with targeted toxicological screening. Full article
(This article belongs to the Special Issue Microgreens—a New Trend in Plant Production)
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16 pages, 4498 KB  
Article
Decoding Mandarin Action Verbs from EEG Using a Dual-LSTM Network: Towards Practical Assistive Brain–Computer Interfaces
by Binshuo Liu, Gengbiao Chen, Lairong Yin and Jing Liu
Sensors 2026, 26(9), 2749; https://doi.org/10.3390/s26092749 - 29 Apr 2026
Abstract
Electroencephalogram (EEG)-based brain–computer interfaces (BCIs) offer a promising pathway for restoring communication. Decoding tonal languages like Mandarin from EEG remains challenging due to homophones and complex temporal dynamics. This study investigates the decoding of six high-frequency Mandarin action verbs—Chi (eat), He (drink), Chuan [...] Read more.
Electroencephalogram (EEG)-based brain–computer interfaces (BCIs) offer a promising pathway for restoring communication. Decoding tonal languages like Mandarin from EEG remains challenging due to homophones and complex temporal dynamics. This study investigates the decoding of six high-frequency Mandarin action verbs—Chi (eat), He (drink), Chuan (wear), Na (take), Kan (look), and Dai (put on)—from EEG signals. We designed a visual-cue-based overt speech production experiment and collected EEG data from 30 participants during visually guided verb reading aloud. A recurrent neural network framework incorporating dual Long Short-Term Memory (LSTM) layers was implemented to model the long-range temporal dependencies in EEG patterns. The proposed model was compared against a traditional Common Spatial Pattern combined with Support Vector Machine (CSP-SVM) baseline. Our LSTM-based model achieved an average classification accuracy of 69.93% ± 3.07% for the six-class task, significantly outperforming the CSP-SVM baseline (36.53% ± 3.17%). Accuracy exceeded 75% under specific training conditions, including more than 15 training repetitions and a training-data proportion of 38%. Furthermore, the model attained this performance level utilizing approximately 38% of the available trial data for training, demonstrating data efficiency. The results indicate that the LSTM architecture can effectively capture the neural signatures associated with Mandarin verb processing, providing a foundation for developing practical EEG-based assistive communication technologies. The inference latency of the trained model, quantified as the post-training per-trial testing time, was under 2 s, supporting near-real-time applications. Full article
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52 pages, 887 KB  
Review
Beyond Blast Injury: Occupational Hygiene, Safety, and Toxicology Considerations for Mixed-Metal and Energetic-Chemical Exposures to Explosive Ordnance Disposal Personnel
by Bryan G. Fry, Kelly Johnstone and Stacey Pizzino
Toxics 2026, 14(5), 379; https://doi.org/10.3390/toxics14050379 - 28 Apr 2026
Abstract
Explosive ordnance (EO), including AXO (abandoned explosive ordnance), IEDs (improvised explosives devices), and UXO (unexploded ordnance), are widely recognised for their blast and fragmentation hazards, but they also represent a persistent and under-addressed source of occupational chemical exposure for explosive ordnance disposal (EOD) [...] Read more.
Explosive ordnance (EO), including AXO (abandoned explosive ordnance), IEDs (improvised explosives devices), and UXO (unexploded ordnance), are widely recognised for their blast and fragmentation hazards, but they also represent a persistent and under-addressed source of occupational chemical exposure for explosive ordnance disposal (EOD) personnel. EOD core activities liberate mixed metals and energetic chemicals, resulting in exposures that are multi-route (inhalation of dusts and fumes, dermal loading amplified by sweat and glove occlusion, and ingestion via hand-to-mouth transfer during eating, drinking, or smoking) and multi-temporal (repeated low-dose background plus task-driven spikes), as well as chemically complex. Clinically, this can present as syndromic overlap across acute and chronic domains, with symptoms that are easily misattributed to heat stress, dehydration, infection, or fatigue. Acute effects of concern include neurotoxic presentations (headache, dizziness, confusion, tremor, and seizure), respiratory and mucosal irritation following dust or fume events, gastrointestinal symptoms, and patterns suggestive of acute hepatic or renal stress, particularly when high-intensity tasks occur in hot environments that compound physiologic strain. Chronic outcomes relevant to repeatedly exposed EOD personnel include renal function decline, neurocognitive effects that can degrade operational decision making and safety, persistent haematologic abnormalities, and endocrine disruption signals, with long-latency risks requiring cautious interpretation given sparse longitudinal data and confounding co-exposures. This review synthesises the current evidence base through an EOD lens and translates it into pragmatic clinical and programmatic actions: task-based exposure characterisation; tiered biomonitoring and medical surveillance aligned to operational tempo; incident-triggered assessment pathways after high-residue events; and prevention strategies that work under field constraints, including contamination control zones, hygiene enforcement, glove and respiratory protection optimisation, tool and vehicle decontamination, and measures to prevent secondary transfer and take-home exposure. The central takeaway is practical: EOD programs can reduce morbidity and improve readiness by treating explosive ordnance as a chemical mixture exposure problem, adopting mixture-aware clinical triage, and embedding surveillance and controls that match how EOD work is actually performed. Full article
14 pages, 516 KB  
Article
When Training Is Not Enough: The Role of Relative Body Mass and Body Image in Predicting Eating Behaviours in Young Judo Athletes—A Companion Cross-Sectional Study
by Paulina Baran, Katarzyna Szczepanik, Łukasz Kapica and Piotr Mamcarz
Obesities 2026, 6(3), 28; https://doi.org/10.3390/obesities6030028 - 28 Apr 2026
Abstract
Judo, as a weight-category combat sport, exposes young athletes to body mass pressures that may foster abnormal eating behaviours. Building on a companion study that documented the prevalence and sex-specific characteristics of abnormal eating behaviours in this cohort, this secondary analysis aimed to [...] Read more.
Judo, as a weight-category combat sport, exposes young athletes to body mass pressures that may foster abnormal eating behaviours. Building on a companion study that documented the prevalence and sex-specific characteristics of abnormal eating behaviours in this cohort, this secondary analysis aimed to identify training-related predictors of eating behaviours in young Polish judo athletes, examine body image satisfaction as a mediator, and assess whether patterns observed in elite adult athletes apply to younger populations. The participants were 150 athletes (70 girls, 80 boys) aged 12–17. Eating behaviours were assessed using the Three-Factor Eating Questionnaire (TFEQ-13) and the Test of Eating Situation Style (TSJ); training characteristics, pre-competition weight control, and appearance satisfaction were examined through hierarchical regression, mediation analysis, latent profile analysis, and two-way ANOVA. Training-related factors—including tenure, session frequency, competitive level, and pre-competition weight control—showed no significant associations with eating behaviours. However, in a subsample of N = 136 athletes, relative weight grouping predicted dietary restraint (p = 0.015, η2p = 0.066), with athletes in the heaviest tertile reporting higher restriction; lower appearance satisfaction was associated with greater restraint (p = 0.031, β = −0.192), independently of sport-mandated weight control; females demonstrated higher emotional eating across instruments (p < 0.001). These findings suggest that body image and weight classification may be more strongly associated with eating behaviours than training demands, highlighting the need for body image interventions and the monitoring of athletes near weight category boundaries. Full article
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