Search Results (513)

Hericium coralloides is a highly valued gourmet and medicinal species with growing market demand across East Asia, though industrial production remains limited by cultivation challenges. This study investigated the molecular characteristics, biological traits, domestication potential, and cultivation protocols of Hericium coralloides strains collected from the Changbaishan Nature Reserve (Jiling, China). Optimal conditions for mycelial growth included mannose as the preferred carbon source, peptone as the nitrogen source, 30 °C incubation temperature, pH 5.5, and magnesium sulfate as the essential inorganic salt. The fruiting bodies had a protein content of 2.43% g/100 g (fresh sample meter). Total amino acids comprised 53.3% of the total amino acid profile, while essential amino acids accounted for 114.11% relative to non-essential amino acids, indicating high nutritional value. Under optimized domestication conditions—70% hardwood chips, 20% cottonseed hulls, 8% bran, 1% malic acid, and 1% gypsum—bags reached full colonization in 28 days, with a 15-day maturation phase and initial fruiting occurring after 12–14 days. The interval between flushes was 10–12 days. The average yield reached 318.65 ± 31.74 g per bag, with a biological conversion rate of 63.73%. These findings demonstrate that Hericium coralloides possesses significant potential for edible and commercial applications. This study provides a robust theoretical foundation and resource reference for its artificial cultivation, supporting its broader industrial and economic utilization. Full article

During a prolonged domestication and environmental selection, Brassica rapa has formed diverse morphological types during a cultivation process of up to 8000 years, such as root-type turnips (Brassica rapa var. rapa), leaf-type Chinese cabbage (Brassica rapa var. pekinensis), oil-type rapeseed (Brassica rapa L.), and other rich types. China is one of the origins of Brassica rapa L., which is spread all over the east, west, south, and north of China. Studying its origin and evolution holds significant importance for unraveling the cultivation history of Chinese oilseed crops, intraspecific evolutionary relationships, and the utilization value of genetic resources. This article summarizes the cultivation history, evolution, classification research progress, and germplasm resource diversity of Brassica rapa var. oleifera in China. Combining karyotype analysis, genomic information, and wild relatives of Brassica rapa var. oleifera discovered on the Qinghai–Tibet Plateau, it is proposed that Brassica rapa var. oleifera has the characteristic of polycentric origin, and Gansu Province in China is one of the earliest regions for its cultivation. Brassica rapa var. oleifera, originating from the Mediterranean region, was diffused to the East Asian continent through two independent transmission paths (one via the Turkish Plateau and the other via Central Asia and Siberia). Analyzing the genetic diversity characteristics and evolutionary trajectories of these two transmission paths lays a foundation for clarifying the origin and evolutionary process of Brassica rapa var. oleifera and accelerating the breeding of Brassica rapa var. oleifera in China. Despite existing research on the origin of Brassica rapa L., the domestication process of this species remains unresolved. Future studies will employ whole-genome resequencing to address this fundamental question. Full article

Despite increasing interest in including indigenous and local people in forest restoration initiatives, their views on which species are most useful, or reasons behind not planting native tree species are often ignored. Focused on south-western Rwanda, this study addressed these knowledge gaps. We carried out 12 focus group discussions with village elders to determine the following: main benefits provided by native forests, the native species they prefer for different uses, and the main barriers to species’ cultivation. Then, considering other key information from the literature, we performed a ranking exercise to determine which native species had the greatest potential for large-scale tree planting initiatives. Our results show that native forests provide 17 benefits to local communities, some of which cannot be replaced by plantations with exotic species. Among the 26 tree species identified as most useful for timber, firewood, medicine and fodder, ten were ranked as with the greatest potential for restoration initiatives. Of these, two had not been included in recent experimental plantations using native species in Rwanda, and none were considered among the priority species for domestication in Africa. Overall, our study highlights the need to better connect the ecological and social dimension of forest reforestation initiatives in multiple contexts. Full article

Sorghum is a versatile crop that serves as a major source of food, feed, fodder and biofuel globally. Lignin content in sorghum affects multiple important traits, including lodging resistance, forage digestibility and the efficiency of bioenergy production. However, the genetic regulation of lignin content in sorghum remains poorly understood. In this study, we combined transcriptomic and comparative genomic approaches to uncover the genetic network underlying lignin biosynthesis in sorghum. Through comparative genomic analysis, we identified 104 candidate genes involved in lignin biosynthesis. Transcriptome analysis of four sorghum accessions with contrasting lignin contents identified 6132 differentially expressed genes with an enrichment of genes related to phenylpropanoid biosynthesis and cell wall biogenesis. The 104 lignin biosynthesis candidates were significantly enriched (p-value < 0.01) in these differentially expressed genes, with most differentially expressed candidate genes related to monolignol biosynthesis and polymerization being up-regulated in high-lignin accessions. These up-regulated genes are related to all key enzymes involved in lignin biosynthesis, suggesting that the elevated lignin content in these accessions results from a collective increase in enzyme activity. Sequence analysis revealed a significant reduction in genetic diversity across lignin biosynthesis genes in cultivated sorghum compared to wild sorghum. Moreover, selection signals during domestication were identified in 30 lignin biosynthesis genes, 22 of which were differentially expressed, further supporting the functional relevance of these differentially expressed genes in lignin biosynthesis. Overall, our findings uncover the lignin biosynthesis gene network in sorghum and offer potential targets for future functional studies and trait manipulation. Full article

Population growth has led to an increased volume of wastewater from industrial, domestic, and municipal sources, contaminating aquatic bodies in the state of Veracruz. This study aimed to assess the efficacy of a water treatment system incorporating a DAF stage, followed by the cultivation of a microalgal consortium to eliminate pollutants from the blended effluent. The cultivation of Nannochloropsis oculata in wastewater entailed the assessment of a single variable (operating pressure) within the DAF system, in conjunction with two supplementary variables (residence time and F:M ratio), resulting in removal efficiencies of 70% for CODt, 77.24% for CODs, 78.34% for nitrogen, and 77% for total organic carbon. The water sample was found to contain elevated levels of organic matter and pollutants, beyond the permitted limits set forth in NOM-001-SEMARNAT-2021. The obtained removal percentages indicate that the suggested physicochemical–biological process (DAF-microalgae) is a suitable method for treating mixed wastewater. This approach reduces atmospheric pollution by sequestering greenhouse gases such as carbon dioxide through the photosynthetic activity of N. oculata cells, so facilitating the production of oxygen and biomass while limiting their accumulation in the atmosphere. Full article

Decentralized sanitation in rural areas urgently requires accessible and nature-based solutions to achieve Sustainable Development Goal 6 (clean water and sanitation for all). However, monitoring studies of such ecotechnologies in disperse communities remain limited. This study evaluated the performance of an evapotranspiration tank (TEvap), designed with community participation, for the treatment of domestic sewage in a rural Quilombola household in the Brazilian Cerrado. The system (total area of 8.1 m², with about 1.0 m² per inhabitant) was monitored for 218 days, covering the rainy season and the plants’ establishment phase. After 51 days, the TEvap reached operational equilibrium, maintaining a zero-discharge regime, and after 218 days, 92.3% of the total system inlet volumes (i.e., 37.47 in 40.58 m³) were removed through evapotranspiration and uptake by cultivated plants (Musa spp.). Statistical analyses revealed correlations that were moderate to strong, and weak between the blackwater level and relative humidity (Pearson correlation coefficient, r = 0.75), temperature (r = −0.66), and per capita blackwater contribution (r = 0.28), highlighting the influence of climatic conditions on system efficiency. These results confirm the TEvap as a promising, low-maintenance, and climate-resilient technology for decentralized domestic sewage treatment in vulnerable rural communities, with the potential to support sanitation policy goals and promote public health. Full article

The domesticated species currently available in the market have been developed through the breeding of wild relatives. Breeding strategies using wild relatives with high genetic diversity are attracting attention as an important approach for addressing climate change and ensuring sustainable food supply. However, studies examining gene expression variation in multiple wild and domesticated species are limited. Therefore, we aimed to investigate the changes in gene expression associated with domestication. We performed a meta-analysis of public gene expression data of domesticated species of rice, tomato, and soybean and their presumed ancestral species using 21 pairs for rice, 36 pairs for tomato, and 56 pairs for soybean. In wild relatives, the expression of genes involved in osmotic, drought, and wound stress tolerance was upregulated, with 18 genes included in the top 5% of DW scores. In domesticated species, upregulated expression was observed in genes related to auxin and those involved in the efflux of heavy metals and harmful substances, with 36 genes included in the top 5% of DW scores. These findings provide insights into how domestication influences changes in crop traits. Thus, our findings may contribute to rapid breeding and the development of new varieties capable of growing in harsh natural environments. Hence, a new cultivation method called “de novo domestication” has been proposed, which combines the genetic diversity of currently unused wild relatives and wild relatives with genome editing technologies that enable rapid breeding. Full article

Trichoderma spp., Metarhizium spp., and Bacillus spp. are commonly used as biocontrol microorganisms domestically and internationally. However, microbial pesticides currently prepared from single living microorganisms have problems such as a short shelf life, particularly under stressful environment conditions. Secondary metabolites produced from biocontrol microorganisms are comparatively stable when used under field conditions. This study screened the optimal combination of biocontrol metabolites, referred to as TMB, composed of culture filtrates from certain isolates of Trichoderma asperellum 10264, Bacillus subtilis S4-4-10, and Metarhizium anisopliae 3.11962 (1:4:1 (v/v)). RNA-seq analysis and transmission electron microscope observations were carried out to identify the major functions of the most effective culture filtrates against Magnaporthe oryzae (the pathogen causing rice blast disease) and Chilo suppressalis (an insect pest in rice cultivation). TMB was found to disrupt the midgut subcellular structure of C. suppressalis larvae and inhibit the expression of genes related to immunity, membrane components, protein synthesis, and other functions in C. suppressalis larvae and M. oryzae, thereby interfering with their normal growth, reproduction, and infection potential in rice. In addition, TMB was also able to promote rice growth and trigger host defense responses against infections by the target pests and pathogens. In summary, TMB generated different inhibitory activities against multiple targets in C. suppressalis and M. oryzae and induced plant immunity in rice. Therefore, it can be used as a new environmentally friendly agent or alternative to control rice pests and diseases. Full article

The common bean is a widely cultivated food source. Many domesticated species of common bean varieties, known as landraces, are cultivated in Mexico by local farmers, exhibiting various colorations and seed mixtures as part of agricultural practices. In this work, we propose a methodology for classifying bean landrace samples using three two-dimensional histograms with data in the CIE L*a*b* color space while additionally integrating chroma (C*) and hue (h°) to develop a new proposal from histograms, employing deep learning for the classification task. The results indicate that utilizing three histograms based on L*, C*, and h° brings an average accuracy of 85.74 ± 2.37 compared to three histograms using L*, a*, and b*, which reported an average accuracy of 82.22 ± 2.84. In conclusion, the new color characterization approach presents a viable solution for classifying common bean landraces of both homogeneous and heterogeneous colors. Full article

An adequate food supply is a core issue for sustainable development worldwide. Amid greater instability in the food supply triggered by more armed conflicts, trade disputes, and climate change, a decline in grain cultivation area still plagues many regions. China, a major food producer globally, is a case in point. The truth is that at the moment, the formulation and implementation of policies as well as academic discussions regarding this issue are predominantly based on the sown area of grains, overlooking the fundamental role co-played by population, yield efficiency, and sown area in determining food supply. Furthermore, the commonly used indicator, the non-grain cultivation rate, fails to directly reflect the impact of the phenomenon on the grain supply. To address these gaps, this study introduces trend-change detection and factor-contribution analysis, uses long-term grain sown area data to identify regions with significant grain retreat, and quantifies the relative influence of population shifts, crop yield improvements, and sown area changes on food supply. Key findings include the following: China’s total grain production maintained steady growth from 2003 to 2023, far exceeding conventional food security thresholds. Temporary reductions in grain sown area (2015–2019, 2021–2022) were offset by rising yields, with no substantial decline in supply. Twelve provinces/municipalities, Beijing, Shanghai, Zhejiang, Fujian, Guangdong, Guangxi, Guizhou, Shaanxi, Ningxia, Sichuan, Chongqing, and Hainan, exhibited substantial declines in grain plantation. However, Sichuan and Shaanxi achieved counter-trend growth in food supply, while Ningxia and Guizhou experienced frequent fluctuations. The sown area was not always the dominant factor in per capita grain availability. Yield increases neutralized cropland reduction in Sichuan, Shaanxi, Guizhou, and Ningxia, whereas population inflows outweighed the sown area effect in the other eight provinces. The study concludes that China’s grain cropland reduction has not yet posed a threat to national food security. That said, the spatial concentration of these affected regions and their ongoing output reductions may raise domestic grain redistribution costs and intensify inter-regional conflicts over cropland protection. Meanwhile, population influx plays a similarly important role to that of grain plantation decline in the grain supply. Considering that, we believe that more moderate measures should be adopted to address the shrinkage of grain planting areas, with pre-set food self-sufficiency standards. These measures include, but are not limited to, improving productivity and adopting integrated farming. Methodologically, this work lowers distortions from normal annual cropland fluctuations, enabling more precise identification of non-grain production zones. By quantifying the separate impacts of population, crop yield, and sown area changes, it supplements existing observations on grain cropland decline and provides better targeted suggestions on policy formulation and coordination. Full article

The process of obtaining Agave L. fibers dates back to pre-Hispanic times, and although humans have obtained different products from this crop, to date, the impact of humans (artificial selection, domestication and intensive cultivation) on these species is unknown. In this study, the expression of the CesA gene was evaluated in three species, namely, Agave L, A. sisalana Perrine and A. fourcroydes Lem. (Sac ki), both of which are used for fiber production, and Agave tequilana Weber. The results revealed that, compared with A. fourcroydes and A. tequilana, A. sisalana had a greater leaf area, a significantly greater cellulose content and a greater number of cellulose fibrils. In terms of cell organization, the number and size of sclerenchyma fibers were similar between A. sisalana and A. fourcroydes. However, the relative expression of the CesA gene was five times greater in A. fourcroydes than in A. sisalana and A. tequilana, in contrast with the number of copies in those genomes. In addition, the tertiary structure of the CESA protein in fiber-producing species was modeled, placing agaves in a group along with Populus, Linum, Corchorus and Boehmeria. The haplotype network analysis revealed that A. tequilana is closely grouped with species of the order Poales, unlike the rest of the fiber-producing agaves, which formed a unique cluster. These findings suggest that artificial selection by humans, for various purposes, has contributed to the specialization of genes associated with traits such as fiber production. Full article

The rhizospheric microbial community plays a crucial role in the growth and ecological adaptation of truffles. Although extensive research has been conducted on bacterial communities in truffle habitats, the spatial variation and functional implications across different regions and soil compartments remain poorly understood in the current literature. In this study, soil bacterial communities were analyzed using 16S rRNA high-throughput sequencing across truffle-producing (Tuber sinense) and non-producing sites in Quercus aliena forests in Panzhihua, China. To capture microhabitat-level variation, soils were classified into three compartments: rhizosphere soil tightly adhering to ectomycorrhizal roots (TRS), rhizoplane soil loosely attached to roots (TRE), and bulk soil from truffle zones without visible roots (TBS), with corresponding controls (RS, RE, BS) collected from truffle-free forests. An alpha diversity analysis revealed that truffle-producing soils harbored significantly higher bacterial richness than control soils, while beta diversity indicated more clustered community composition in truffle-associated soils. A taxonomic analysis showed that T. sinense occurrence was associated with enrichment of specific bacterial taxa, including Chloroflexi, Anaeromyxobacteraceae, and Bradyrhizobium, whereas widespread taxa such as Firmicutes and Bacteroidota were more abundant in control soils. To further identify microbial indicators closely associated with truffle presence, we employed random forest modeling, which highlighted Pseudomonas, Streptomyces, and Bradyrhizobium as key genera distinguishing truffle-associated rhizospheres. These findings suggest that T. sinense may influence the composition of rhizospheric microbial communities, thereby constructing a favorable rhizospheric microenvironment. This work provides new insights into the microbial ecology of T. sinense and lays a foundation for future truffle domestication and cultivation efforts. Full article

Cucurbita L. is a valuable gourd vegetable crop, with high nutritional and economic value. However, the lack of a molecular identification system and population genetic information has impeded the development of proper conservation strategies and marker-assisted genetic breeding for Cucurbita varieties. In this study, we developed a set of simple sequence repeat (SSR) markers for distinguishing the main cultivated Cucurbita cultivars in China and providing technical support for domestic variety preservation, registration, and intellectual property protection. A total of 152 allelic variations and 308 genotypes were identified from 306 Cucurbita cultivars by using 24 SSR markers. Using 24 core markers, we successfully distinguished 300 varieties from 306 Cucurbita varieties, and the identification rate reached 98.36%. The PIC values of the 24 core markers ranged from 0.281 to 0.749, and the average value was 0.643, which was considered high genetic diversity. Based on the results of cluster analysis, principal component analysis, and population genetic structure analysis using 24 pairs of core primers for 306 Cucurbita varieties, the results were basically consistent, all categorized into three genetic clusters, corresponding to the three species: C. moschata, C. pepo, and C. maxima. These results showed a certain correlation with phenotypic traits. DNA fingerprints were constructed for the 306 Cucurbita cultivars based on the core markers. Our research results provide a new tool for population genetic analysis, variety identification, and protection in Cucurbita cultivars with high efficiency, accuracy, and lower costs compared to conventional methods. Full article

Common beans (Phaseolus vulgaris) are an important source of protein for the Brazilian population. They are cultivated all over the country, in three cropping seasons/year, totaling 2.7 million tons, mostly for domestic consumption. Pest management is a big challenge and is mostly carried out with the intensive use of pesticides. Integrated pest management (IPM) is essential for sustainability. This technology is based on applying insecticides only when the pest population reaches the Economic Threshold. For that, it is necessary to monitor the crop for the occurrence of pests and beneficial arthropods. Although the concept of IPM and its benefits have long been known and widespread, it is not clear whether bean producers adopt the technology, since informal reports suggest that preventive insecticide applications are still highly used in the crop. The objective of this study was to survey the level of IPM adoption among bean producers in different regions of Brazil, using a questionnaire, applied to 103 producers/consultants. The results show that the estimated rate of IPM adoption by common bean producers in Brazil is 46.6%. The main causes of the low adoption are a lack of understanding of IPM concepts, high confidence in the efficiency of pesticides, and high costs of crop monitoring. Full article

With rapid globalization and the increasing demand for traditional Chinese medicines, quality and safety has become a critical priority for both domestic and international markets. However, traditional Chinese medicines are susceptible to contamination by pathogenic microorganisms during the process of cultivation, growth, processing, storage, and transportation, which can lead to mycotoxin contamination that adversely affect the quality and safety of traditional Chinese medicines, and may pose potential threats to human health. This review summarizes mycotoxin contamination, the common detection methods, prevention and control measures, and regulatory recommendations, aiming to provide references for improving the quality standards and ensuring safety of these medications. Full article