Search Results (6,989)

Background: Multiple sclerosis (MS) is a chronic, inflammatory, and demyelinating disease of the central nervous system that predominantly affects young adults, particularly women, and is associated with progressive disability and a wide range of symptoms that impair functionality and quality of life. Recent research suggests that diet, especially its inflammatory potential, may influence the clinical course of the disease. We hypothesize that patients following a proinflammatory dietary pattern will exhibit poorer functional outcomes than those following an anti-inflammatory diet. Methods: An observational preliminary study was conducted, including 19 patients. Dietary inflammatory potential was assessed using the Dietary Inflammatory Index (DII), while functional status was measured with the Functional Assessment of MS (FAMS) scale. Results: Participants were divided into two groups according to their DII score: a group following a more pro-inflammatory diet (n = 10; 80% female; mean age 49.60 ± 10.63 years) and a group following a more anti-inflammatory diet (n = 9; 44.4% female; mean age 49.00 ± 10.79 years). The results show that patients with a proinflammatory dietary profile reported a higher symptom burden (FAMS symptoms score 20.70 ± 5.48 vs. 14.44 ± 7.05, p = 0.044), including greater fatigue as well as musculoskeletal and gastrointestinal complaints. In contrast, patients with an anti-inflammatory dietary profile reported fewer symptoms, greater energy and vitality, and higher intake of anti-inflammatory nutrients such as fiber, magnesium, and vitamin B6. No other significant between-group differences were observed. Conclusions: These findings suggest that dietary interventions aimed at reducing inflammation may improve functionality and quality of life in persons with MS. However, given the limited sample size, larger multicenter longitudinal studies are required to confirm these results. The findings of this study may provide preliminary evidence to inform future research. Full article

Background: Cardiometabolic diseases are among the leading causes of mortality worldwide and are strongly influenced by lifestyle factors. Objective: The aim of this study was to determine the association between a healthy lifestyle and cardiometabolic risk in higher education students in the San Martín region, Peru. Methods: A cross-sectional study with non-probabilistic convenience sampling was conducted among 1054 students from higher education institutions in the San Martín region, Peru. The Healthy Diet and Lifestyle Scale (DEVS) was applied, and anthropometric indicators (BMI and waist circumference) were assessed by trained nutritionists. Descriptive statistics, association tests (chi-square and Wilcoxon), and Poisson regression models with robust variance were used. Results: Obesity was significantly associated with higher vitamin B12 intake (PR = 2.39; 95% CI: 1.16–4.91) and with higher water consumption (>8 glasses/day) (PR = 2.61; 95% CI: 1.20–5.66), although these findings may reflect reverse causality given the cross-sectional nature of the data. Greater whole grain consumption was associated with a lower risk of elevated waist circumference (PR = 0.60; 95% CI: 0.40–0.91). Similarly, engaging in ≥30 min of daily physical activity was significantly associated with reduced central adiposity (PR = 0.69; 95% CI: 0.56–0.86). Conclusions: The findings suggest that whole grain consumption and regular physical activity act as protective factors, whereas certain dietary patterns—despite being considered healthy—may be associated with higher cardiometabolic risk depending on the dietary context. Full article

Background: The placenta stands at the maternal–fetal interface and is a key organ regulating the intrauterine environment. In pregnancies exposed to obesity, placental function, signaling, and nutrient handling are adversely altered. Pre-conception weight loss is a potential intervention to alter an obesogenic milieu of pregnancy, which we investigated in a mouse model of maternal obesity using diet or administration of the glucagon-like peptide-1 (GLP-1) receptor agonist liraglutide. Methods: Pre-pregnancy weight loss in C57BL/6 high-fat diet (HFD)-fed dams was induced in the pre-pregnancy period by switching diet from HFD to chow diet or administering liraglutide (0.3 mg/kg/day subcutaneously for 4 weeks) whilst continuing HFD. In addition, a group of HFD-fed dams were switched to chow diet post-conception. The metabolomic profile and gene expression within the placenta was compared at day 18–20 of gestation. Results: ¹H NMR spectroscopy metabolomic analysis of placenta of HFD mice showed an altered amino acid metabolomic profile, with lower aspartate, glutamate, and glutamine levels compared to the placenta of chow-fed mice (p < 0.05). Meanwhile, gene expression analysis identified both oxidative stress and inflammation in the placentas of HFD-fed dams. Whilst dietary modification alone was sufficient to reduce markers of oxidative stress and inflammation, liraglutide treatment modulated pathological changes, including placental metabolic stress but not inflammation. Conclusions: These findings highlight the importance of dietary or pharmacological interventions in the pre- or immediate post-conception period, with pre-conception offering a critical window to reduce aberrant placental changes induced by obesity. Full article

Purpose: This study investigated the effects of dietary bamboo leaf extract (BLE) on milk parameters and intestinal microbiota in lactating Yili mares. Methods: Twenty-four Yili mares of similar age (10 ± 2 years), weight (360.62 ± 15.23 kg) and body condition were selected for this study and randomly divided into four groups of six mares each: an untreated control group (CG) and three experimental groups (EG1, EG2, EG3) were fed a basal diet supplemented with 0, 10, 20, or 30 g/day of BLE, respectively, for 60 days. Then, horse milk composition, antioxidant activity, and immunoglobulin levels along with the relative abundance of fecal microbiota were measured. Results: Compared with the control group, supplementation with BLE for 60 days significantly improved milk yield and composition. The protein content in the EG1 was significantly higher than that in the CG, the milk yield and fat content in the EG2 was significantly higher than that in the CG, and the lactose content in the EG3 was significantly higher than that in the CG. BLE also significantly increased the milk’s antioxidant capacity, vitamin C, IgG, IgM, and IgA levels, with the antioxidant and immune properties in the EG2 being significantly higher than those in the CG. Furthermore, BLE feeding promoted communities of beneficial intestinal microbes. Bacteria associated with energy metabolism and organic matter decomposition increased significantly in BLE-fed groups, especially the EG2, which had elevated abundance of UCG-002 and the NK4A214_group. BLE also significantly reduced the abundance of Euryarchaeota, Verrucomicrobiota, Methanobacteriaceae, and Methanobrevibacter. Conclusions: Dietary supplementation with bamboo leaf extract is a safe and inexpensive way to enhance milk yield and quality and to promote the growth of beneficial intestinal microbes in Yili horses. Full article

Background/Objectives: Childhood obesity remains a major global health challenge influenced by poor dietary patterns and excessive energy intake. Understanding gender-specific nutritional deviations may improve the effectiveness of preventive and therapeutic interventions. This study aimed to evaluate differences in energy and nutrient intake between boys and girls with overweight or obesity status and to identify the main food sources contributing to these differences. Methods: Data from a total of 180 participants (83 boys, 97 girls; 7–18 years) attending the national obesity intervention program Camp My Challenge was analyzed. Anthropometric parameters and dietary intake were assessed using a validated food-frequency questionnaire (OPKP). Intakes were compared with Slovenian dietary reference values (DRVs). Group differences were tested using ANOVA, t-tests, and Pearson correlations (p < 0.05). Results: Boys exhibited higher body mass (79.9 ± 22.6 kg vs. 69.2 ± 19.1 kg; p = 0.001) and BMI (30.8 ± 4.8 kg/m² vs. 28.5 ± 4.4 kg/m²; p = 0.001). Mean energy intake was 2543 ± 1138 kcal/day, exceeding DRV by 16% (t = 3.31, p < 0.001). Girls exceeded energy requirements by 24.5% vs. 5.4% in boys (p = 0.019). Boys consumed significantly more total fat (106 ± 61 g vs. 85 ± 47 g; p = 0.014), saturated fatty acids (34 ± 20 g vs. 27 ± 13 g; p = 0.011), protein (119 ± 63 g vs. 98 ± 41 g; p = 0.008), and sodium (3628 ± 2086 mg vs. 2852 ± 1520 mg; p = 0.005). Girls showed higher sugar intake (208% vs. 166% of DRV; p = 0.032), mainly from sweet foods (24%) and fruit (26%), whereas beverages—predominantly isotonic drinks—accounted for 27% of boys’ sugar intake. Sugar intake correlated with waist circumference (r = 0.305, p = 0.002) and fat mass (r = 0.272, p = 0.007) in girls. Sodium intake exceeded DRV sixfold in both sexes. Conclusions: Marked sex-specific dietary disparities exist among children with obesity. Interventions should target high sugar and energy intake in girls and excessive fat, sodium, and sugar-sweetened beverage consumption in boys to enhance the efficacy of pediatric obesity management. Full article

Weaned piglets have a fragile gastrointestinal tract and immature digestive function. Supplementation of Bacillus can enhance intestinal barrier function and improve nutrient digestion and absorption efficiency. It is an important nutritional regulation method to alleviate weaning stress, reduce the incidence of diarrhea and promote growth performance. This experiment was conducted to investigate the effects of Bacillus subtilis, Bacillus pumilus and their combination on growth performance, diarrhea incidence, nutrient apparent digestibility, intestinal morphology and barrier function of weaned piglets. A total of 128 weaned piglets weighing 6.68 kg (±0.35 kg) were selected, divided into 4 treatment groups, and fed with a basal diet (CTR), a Bacillus subtilis (BS1), a Bacillus pumilus (BS2) and a Bacillus subtilis + Bacillus pumilus (BS1 + BS2) for 42 days. Each group had 8 replicates with 4 piglets per replicate. One piglet was selected from each replicate and euthanized to collect intestinal samples. The results showed that compared with the CTR group, the BS1 + BS2 group significantly increased the average daily gain (ADG) of weaned piglets on days 0–14 (p < 0.05), and the BS2 group significantly increased the ADG on days 0–42 (p < 0.05). Compared with the CTR group, the BS1 + BS2 group significantly reduced the full-time diarrhea rate (p < 0.05), and weaned piglets of the BS2 group significantly reduced the incidence of diarrhea on days 0–14, 15–28, and 0–42 of the study, in comparison to the control (p < 0.05). Compared with the CTR group, the piglets in the BS1 + BS2 group significantly decreased the serum tumor necrosis factor-α (TNF-α) content on day 21 (p < 0.05), and the BS1, BS2, and BS1 + BS2 groups significantly decreased the serum TNF-α content on day 42 (p < 0.05). Compared with the CTR group, the BS1 + BS2 group significantly reduced the expression of Interleukin-8 (IL-8) mRNA in the ileum (p < 0.05). The BS1, BS2, and BS1 + BS2 groups significantly reduced the expression of TNF-α mRNA in the ileum, IL-8 mRNA in the jejunum, and TNF-α mRNA in the jejunum (p < 0.05). In addition, compared with the CTR group, the BS2 and BS1 + BS2 groups significantly increased Claudin-1 mRNA expression in the jejunum (p < 0.05). Compared with the CTR group, the BS1 and BS2 groups significantly increased Occludin mRNA expression in the jejunum (p < 0.05). In summary, dietary supplementation with Bacillus-based probiotics can significantly improve growth performance in weaned piglets, reduce diarrhea incidence, alleviate inflammation, and enhance intestinal barrier function. Full article

Background: Field hockey is a high-intensity intermittent sport, where nutrition and sleep play an important role in the performance and health of all players, especially in women, who often exhibit a dietary pattern characterized by low energy and carbohydrate intake, along with poor nighttime habits. The purpose of this study is to evaluate the profile of female field hockey players by analyzing their energy and macronutrient intake, prevalence of LEA risk, use of SS, and sleep characteristics. Methods: A cross-sectional, observational, and descriptive study was carried out with 75 female players. Validated questionnaires were used to determine general sports nutrition knowledge (NUKYA) and specific knowledge of carbohydrates in sports (CEAC-Q), sleep quality (ASSQ), low energy availability risk (LEAF-Q), and use of sport supplements. In addition, a 7-day dietary record was analyzed. Results: Players showed a high general nutrition knowledge (NUKYA mean: 66.0 ± 8.5 points; 68.0% scored high), but a pronounced deficiency in specific carbohydrate knowledge (CEAC-Q mean: 24.3 ± 14.9 points; 84.0% scored low). Dietary intake analysis revealed significant deficiencies: mean energy intake was 31.9 ± 10.8 kcal/kg/day, resulting in insufficient intake for 78.7% of players. Carbohydrate intake was particularly low (3.6 ± 1.5 g/kg/day), with 86.7% failing to meet recommendations. Furthermore, 33.3% of players were at risk of Low Energy Availability (LEA). LEA risk (LEAF-Q score) was negatively correlated with both protein (r = −0.363; p = 0.001) and carbohydrate intake (r = −0.347; p = 0.003). Regarding sleep, the mean disturbance score (SDS) was 6.5 ± 2.9 (mild disturbance), with 33.3% showing moderate–severe disturbance, and 92.0% sleeping ≤8 h. Finally, 78.7% of players used supplements, with usage correlated with CEAC-Q scores (r = 0.233; p = 0.044), and 86.4% were guided by non-professional sources. Conclusions: Female hockey players do not meet dietary recommendations for energy and carbohydrates and exhibit a high prevalence of low energy availability and sleep disturbances, despite having acceptable general sports nutrition knowledge. It is recommended to implement specific educational and nutritional strategies to enhance the knowledge, performance, and health of female hockey players. Full article

Background/Objectives: The impact of dietary adherence and formula intake regularity on fat-soluble vitamin status in phenylketonuria (PKU) is uncertain. This study assessed whether vitamin A, D, E, and beta-carotene levels differ by dietary adherence and regularity of Phe-free formula intake. Methods: A cross-sectional study included 98 individuals (age 6–41 years) with vitamin D measurements. In a subgroup of 68 patients, vitamin A, vitamin E, and beta-carotene levels were determined. Vitamin levels were compared between adherent and non-adherent groups and between participants with regular vs. irregular formula intake. A subsequent systematic review and meta-analysis of six studies (from PubMed, Scopus, Web of Science, and Cochrane; searched in August 2025) pooled standardised mean differences (SMDs) using fixed-effects and random-effects models. Results: The cross-sectional results showed higher vitamin D in adherent (35.60 [30.39–41.65] vs. 32.90 [26.50–40.00] ng/mL, p = 0.034) and regular formula consumers (35.97 [30.03–42.28] vs. 30.20 [26.08–35.06] ng/mL, p = 0.002). Beta-carotene was elevated with regular intake (74.40 [56.70–98.45] vs. 53.20 [34.10–68.60] ng/mL, p = 0.003). Meta-analysis confirmed higher vitamin D in adherent individuals (fixed-effects model, SMD = 0.290, 95% CI: 0.004, 0.576, p = 0.047) and regular consumers (fixed-effects model, SMD = 0.750, 95% CI: 0.382, 1.118, p < 0.0001). No differences were observed for vitamin E or beta-carotene. Conclusions: Adherence to diet and regular formula intake is associated with improved vitamin D status, underscoring the critical role of fortified formulas in PKU management. The very low certainty of evidence necessitates further research, especially for the other fat-soluble vitamins. Nonetheless, clinical practice should emphasise support for adherence and ongoing nutritional monitoring. Full article

Background: Neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease, are among the leading causes of disability and mortality worldwide. Dietary patterns have emerged as modifiable risk factors that may influence disease onset and progression. The Mediterranean diet (MedDiet), rich in fruits, vegetables, whole grains, legumes, fish, and extra virgin olive oil, has been consistently associated with better cognitive outcomes and reduced risk of neurodegeneration. Aim: This narrative review summarizes current evidence on the role of the MedDiet in slowing the progression of neurodegenerative diseases, with a particular focus on polyphenols such as resveratrol and oleuropein as key bioactive mediators. Methods: We synthesized findings from epidemiological studies, clinical trials, and mechanistic research to provide an integrated overview of how adherence to the MedDiet and its polyphenol components affects neurodegenerative disease trajectories. Results: Epidemiological studies suggest that higher MedDiet adherence is associated with slower cognitive decline, reduced conversion from mild cognitive impairment to Alzheimer’s disease, and better motor and non-motor outcomes in Parkinson’s disease. Mechanistically, the MedDiet modulates oxidative stress, neuroinflammation, mitochondrial function, vascular health, and the gut–brain axis. Polyphenols such as resveratrol and oleuropein exert neuroprotective effects through antioxidant activity, modulation of amyloid aggregation, mitochondrial biogenesis, and activation of signaling pathways (e.g., SIRT1). Clinical studies, although limited, indicate beneficial effects of polyphenol-rich interventions on cognitive and metabolic biomarkers. Conclusions: Current evidence supports the Mediterranean diet as a promising dietary strategy to slow the progression of neurodegenerative diseases. Polyphenols, including resveratrol and oleuropein, may play a role in mediating these effects. Further well-designed, long-term clinical trials are needed to establish causal relationships, optimize dosage, and explore biomarker-driven personalized nutrition approaches. Full article

Background/Objectives: Excessive social media usage in the current times and high rates of food advertising can impact the health status of individuals by increasing food cues related to perceived hunger and, thus, dietary behaviour. This study examined the association between social media usage patterns, food-related advertising, and dietary choices among Gen Z individuals. Methods: A cross-sectional study was carried out amongst 314 young adults between the ages of 18 and 25 in Surat city, Gujarat. Data was collected for social media usage, the most used platforms, preferred content, and eating patterns. Anthropometric measurements (height and weight) were also recorded. Perceived hunger responses to 12 social media-based food images were assessed using a ten-point Visual Analogue Scale (VAS). Statistical analyses were performed using SPSS (version 26.0), with the significance level set at p < 0.05. Results: YouTube and Instagram were the most used social media apps. There were no significant differences observed between the BMI of participants using social media for 2 h a day and those using it 3+ hours a day. However, a significant association between the BMI of those who viewed advertisements for ready-to-eat foods (p = 0.004) and the BMI of those who viewed advertisements for food delivery platforms (p = 0.001) was seen. A significant difference between usage of Pinterest (p = 0.02), Instagram (p = 0.047), and BMI was also found. Conclusions: Social media marketing and food content are shaping the dietary choices of young adults, and more studies need to be conducted in Pan India to understand the reasons. Such evidence will be crucial for guiding nutrition policies, digital marketing regulations, and youth-focused awareness programmes. Full article

Background: To evaluate the short-term effects of a dietary supplement containing curcumin, bromelain, glucosamine, chondroitin sulphate, sodium hyaluronate, type II collagen, and vitamin C on symptomatic vitreous floaters (SVFs) following Nd:YAG laser capsulotomy. Methods: Forty eyes with SVFs on the first postoperative day were randomized into a control group (standard topical therapy, n = 20) and a treatment group (oral supplement plus standard therapy, n = 20). Outcomes included best-corrected visual acuity (BCVA), contrast sensitivity (CS), and subjective scores from a non-standardized questionnaire on floater perception (QS1), interference with daily activities (QS2), and foreign body sensation (QS3). Objective evaluation was performed using two novel ultrasound-based methods: mean number of vitreous peaks (MVP) from A-scans and mean grey intensity (MGI) from B-scan images processed with ImageJ. Results: At 2 months, the treatment group showed greater improvement in CS (Δ = 0.26 LogCS, CI, 0.14–0.38; p < 0.01), QS1 (Δ = 1.10; 95% CI, 0.60–1.60; p < 0.01), QS2 (Δ = 0.90; 95% CI, 0.40–1.40; p < 0.01), QS3 (Δ = 0.90; 95% CI, 0.44–1.36; p < 0.01), MVP (Δ = 1.10; 95% CI, 0.60–1.60; p < 0.01), and MGI (Δ = 12.89 units; 95% CI, 7.84–17.93; p < 0.01). BCVA was comparable between groups (p = 0.478). Conclusions: Short-term dietary supplementation with vitreous-specific nutrients is well tolerated and associated with improvements in reducing SVFs and foreign body sensations after Nd:YAG capsulotomy and may represent a promising non-invasive therapeutic option. Full article

Thanks to both endogenous and exogenous antioxidants (AOs), the antioxidant defense system ensures redox homeostasis, which is crucial for protecting the body from oxidative stress and maintaining overall health. The food industry also exploits the antioxidant properties to prevent or delay the oxidation of other molecules during processing and storage. There are many classical methods for assessing antioxidant capacity/activity, which are based on mechanisms such as hydrogen atom transfer (HAT), single electron transfer (SET), electron transfer with proton conjugation (HAT/SET mixed mode assays) or the chelation of selected transition metal ions (e.g., Fe²⁺ or Cu¹⁺). The antioxidant capacity (AOxC) index value can be expressed in terms of standard AOs (e.g., Trolox or ascorbic acid) equivalents, enabling different products to be compared. However, there is currently no standardized method for measuring AOxC. Nanoparticle sensors offer a new approach to assessing antioxidant status and can be used to analyze environmental samples, plant extracts, foodstuffs, dietary supplements and clinical samples. This review summarizes the available information on nanoparticle sensors as tools for assessing antioxidant status. Particular attention has been paid to nanoparticles (with a size of less than 100 nm), including silver (AgNPs), gold (AuNPs), cerium oxide (CeONPs) and other metal oxide nanoparticles, as well as nanozymes. Nanozymes belong to an advanced class of nanomaterials that mimic natural enzymes due to their catalytic properties and constitute a novel signal transduction strategy in colorimetric and absorption sensors based on the localized surface plasmon resonance (LSPR) band. Other potential AOxC sensors include quantum dots (QDs, <10 nm), which are particularly useful for the sensitive detection of specific antioxidants (e.g., GSH, AA and baicalein) and can achieve very good limits of detection (LOD). QDs and metallic nanoparticles (MNPs) operate on different principles to evaluate AOxC. MNPs rely on optical changes resulting from LSPR, which are monitored as changes in color or absorbance during synthesis, growth or aggregation. QDs, on the other hand, primarily utilize changes in fluorescence. This review aims to demonstrate that, thanks to its simplicity, speed, small sample volumes and relatively inexpensive instrumentation, nanoparticle-based AOxC assessment is a useful alternative to classical approaches and can be tailored to the desired aim and analytes. Full article

Background/Objective: In recent years, there has been a growing public interest in natural products, including those derived from bees. While most scientific research on bee products has focused on their pharmacological properties, insufficient attention has been given to consumer knowledge, consumption habits and attitudes. The aim of this study was to estimate the popularity of use of different bee products and assess consumer knowledge about them in Lithuania. Methods: An online survey was carried out of the general adult population of Lithuania with 421 respondents included. Results: The study revealed that honey, beeswax and royal jelly were the best-known bee products, while bee venom was the least known one. Knowledge levels varied by age and occupation of respondents—older people and those working in pharmacy, healthcare, cosmetology, agriculture, beekeeping and food production showed better perception. Honey was most often used for treating colds (78.9%), prevention (78.1%) and reducing fever (65.3%). Dietary use of honey depended on demographic factors and was generally low—28.3% consumed it only a few times per year or less. For cosmetics, propolis was the most used product (34.2%). Conclusions: Older individuals and professionals in pharmacy, healthcare, cosmetology, agriculture, beekeeping and food production, demonstrated better knowledge of bee products. Although honey was rarely consumed as part of the diet, older people tended to use it more often than younger individuals. Men were more likely to use honey for treating digestive and circulatory issues and as an ingredient in food and beverages, whereas women were more inclined to use it for respiratory illnesses and cosmetic treatments. Full article

Background/Objectives: Metabolic syndrome (MetS) and even closely related metabolic and anthropometric alterations require timely intervention to reduce associated risks. Neo-Policaptil^® Gel Retard has shown potential in managing both established MetS and early imbalances. To further characterize its real-world impact, a large post-marketing survey was conducted to assess perceived effectiveness, safety, quality of life (QoL), and patterns of use. Methods: Between December 2021 and May 2025, patients/child caregivers, pharmacists, and physicians completed online questionnaires via a dedicated web platform. Patients reported their direct experience, while healthcare professionals (HCPs) provided observations on patient use. Results: The survey included 2204 patients, 57 child caregivers, 455 physicians, and 387 pharmacists. Overall, 97.7% of patients reported an improvement in their condition. Most physicians (94.3%) and pharmacists (89.1%) rated the overall effectiveness of the product as “good” or “excellent”. The safety profile was judged “good” or “excellent” by 84.4% of patients and by over 93% of HCPs. The product was mainly used as monotherapy or in combination with dietary measures and/or physical exercise. Conclusions: These findings underscore the product’s effectiveness, safety, tolerability, and positive influence on QoL in both adults and children affected by MetS or by related metabolic and anthropometric imbalances. Full article

Background: Milk fat metabolism is a complex process regulated by non-coding RNAs. circRNAs (circRNAs) can act as miRNA sponges to regulate target gene expression. Purpose: This study aimed to investigate the role and mechanism of the circRNA_01754/miR-302c/LATS2 axis in regulating milk fat metabolism in bovine mammary epithelial cells (BMECs). Methods: mRNA sequencing was performed on bovine mammary gland tissues from different lactation stages to analyze the expression profiles. The interactions between circRNA_01754, miR-302c, and LATS2 were verified using dual-luciferase reporter assays, qRT-PCR, and Western blot. The functional effects on triglyceride (TAG) and cholesterol synthesis were assessed. Results: CircRNA_01754 was identified as a direct sponge for miR-302c. Overexpression of circRNA_01754 increased LATS2 expression by sequestering miR-302c. Functional experiments showed that circRNA_01754 and LATS2 promoted TAG synthesis, whereas miR-302c inhibited it. Furthermore, miR-302c was found to regulate the expression of YAP1, a key effector of the Hippo pathway, through LATS2. Conclusions: Our findings reveal that circRNA_01754 promotes milk fat synthesis by acting as a ceRNA for miR-302c to upregulate LATS2. This study lays the groundwork for producing high-quality milk and opens up new avenues for enhancing public dietary health. Full article