Search Results (517)

Biofilms formed by Staphylococcus aureus on medical devices and tissue surfaces are a major contributor to persistent infections due to their resistance to antibiotics. Hydrodynamic forces in physiological and device-associated environments significantly influence biofilm development, yet the dynamics of detachment and regrowth under flow remain poorly quantified. In this study, biofilm surface coverage was measured in microfluidic flow assays across combinations of shear rates and nutrient concentrations. A computational workflow was used to segment biofilm trajectories into three kinetic phases—growth, exodus, and regrowth—based on surface coverage dynamics. Each phase was modeled using parametric functions, and fitted parameters were interpolated across experimental conditions to reconstruct biofilm lifecycles throughout the flow–nutrient conditions. The analysis revealed that intermediate shear rates triggered early detachment events while suppressing subsequent regrowth, whereas lower and higher shear regimes favored biofilm persistence. The resulting model enables quantitative comparison of condition-specific biofilm behaviors and identifies key thresholds in mechanical and nutritional inputs that modulate biofilm stability. These findings establish a phase-resolved framework for studying S. aureus biofilms under hydrodynamic stress and support future development of targeted strategies to control biofilm progression in clinical and engineered systems. Full article

The increase in population and demand for the various needs of citizens increases the interaction with the geo-environment. Thus, the rate of natural events affecting daily human life increases. Such an event occurred on a rock cliff in a densely populated area in İstanbul (Türkiye). More than four rock blocks (approximately 3–5 m³) belonging to the Paleozoic sequence of İstanbul, composed of nodular limestone with sandy-clay interlayers, detached and fell. The blocks traveled along a path of approximately 60 m and stopped by crushing a couple of buildings downslope. The path was rough and contained various surface conditions (e.g., bedrock, talus, and plants). This study was initiated by the examination of the dimensions of failed rock blocks, their paths, and topographic conditions. Unmanned vehicles (drones) facilitated the generation of 3D numerical models of topographic changes on the site. Quantifying discontinuity properties (such as persistence, spacing, roughness, etc.) and defining weathering properties comprises the second stage, along with sampling. Based on digital topographic data and field observations, cross-sections were defined by means of possible rockfall areas within the area of potentially unstable blocks. Numerical analysis and rockfall analysis were conducted along these critical sections. Interpretation of laboratory data and results obtained from numerical studies leads to an understanding of the mechanism of the recent rockfall event and demonstrates the most critical areas to be considered and reinforced. The research comprises proposing appropriate reinforcement techniques due to the strong Turkish regulations along the “Bosphorus Waterfront Protected Zone”. The study advises pre-cleaning of potentially unstable blocks after a fence production on paths where rocks could fall, and rock anchors in some localities with varying lengths. The latest part of the research covers the re-assessment of mitigation processes with numerical models, which shows that the factor of safety increased to the desired levels. The reinforcement applications at the site match well with the proposed prevention methods. Full article

Background: Intentional occlusion of the internal iliac artery (IIA) during endovascular repair of aorto-iliac aneurysms may predispose patients to pelvic ischemic complications such as gluteal claudication, erectile dysfunction, and bowel ischemia. Iliac branch devices (IBDs) have been developed to preserve hypogastric perfusion. E-Liac (Artivion/Jotec) is one of the latest modular IBDs yet reports on mid-term performance are limited to small single-center cohorts with short follow-up. The CAMpania PugliA bRanch IliaC (CAMPARI) study is a multicenter investigation of E-Liac outcomes. Methods: A retrospective observational cohort study was conducted across five Italian vascular centers. All consecutive patients undergoing E-Liac implantation for aorto-iliac or isolated iliac aneurysms between January 2015 and December 2024 were identified from prospectively maintained registries. Inclusion criteria comprised elective or urgent endovascular repair of aorto-iliac aneurysms in which an adequate distal sealing zone was not available without covering the IIA and suitability for the E-Liac device according to its instructions for use (IFU). Patients with a life expectancy < 1 year or hostile anatomy incompatible with the IFU were excluded. The primary end point was freedom from branch instability (occlusion/stenosis, kinking, or detachment of the bridging stent). Secondary end points included freedom from any endoleak, freedom from device-related reintervention, freedom from gluteal claudication, aneurysm-related and all-cause mortality, acute renal failure, and sac regression > 5 mm. Results: A total of 69 consecutive patients (68 male, 1 female, median age 72.0 years) received 74 E-Liac devices, including 5 bilateral implantations. The mean infrarenal aortic diameter was 45 mm and the mean CIA diameter 34 mm; 14 patients (20.0%) had a concomitant IIA aneurysm (>20 mm). Concomitant fenestrated or branched aortic repair was performed in 23% of procedures. Two patients received a standalone IBD without implantation of a proximal aortic endograft. Technical success was achieved in 71/74 cases (96.0%); three failures occurred due to inability to catheterize the IIA. Distal landing was in the main IIA trunk in 58 cases and in the posterior branch in 13 cases. Over a median follow-up of 18 (6; 36) months, there were four branch instability events (5.4%): three occlusions and one bridging stent detachment. Seven patients (9.5%) developed endoleaks (one type Ib, two type II, two type IIIa, and two type IIIc). Five patients (6.8%) required reintervention, and five (6.8%) reported gluteal claudication. There were seven all-cause deaths (10%), none within 30 days or related to aneurysm rupture; causes included COVID-19 pneumonia, acute coronary syndrome, melanoma, gastric cancer, and stroke. No acute renal or respiratory failure occurred. Kaplan–Meier analysis showed 92% (95% CI 77–100) freedom from branch instability in the main-trunk group and 89% (60–100) in the posterior-branch group (log-rank p = 0.69). Freedom from any endoleak at 48 months was 87% (95% CI 75–95), and freedom from reintervention was 93% (95% CI 83–98). Conclusions: In this multicenter cohort, the E-Liac branched endograft demonstrated high technical success and favorable early–mid-term outcomes. Preservation of hypogastric perfusion using E-Liac was associated with low rates of branch instability, endoleak, and reintervention, with no 30-day mortality or aneurysm-related deaths. These findings support the safety and efficacy of E-Liac for aorto-iliac aneurysm management, although larger prospective studies with longer follow-up are needed. Full article

To address the inefficiency and high labor intensity associated with traditional manual mussel seedling unloading, this study proposes an automated traction-rope mussel unloading machine. This study focuses on the thick-shelled mussel (Mytilus coruscus) as the research subject. Furthermore, the key mussel unloading processes were simulated using the EDEM software to analyze mechanical interactions during detachment. A breakable mussel discrete element model was developed, and its Bonding V2 model parameters were systematically calibrated. Using the ultimate crushing displacement (2.25 mm) and ultimate crushing load (552 N) as response variables, the model was optimized through a sequential experimental design comprising Plackett–Burman screening, the steepest ascent method, and the Box–Behnken response surface methodology. The results demonstrate that the optimal parameter combination consists of unit area normal stiffness (2.48 × 10¹¹ N/m³), unit area tangential stiffness (3.80 × 10⁸ N/m³), critical normal stress (3.15 × 10⁶ Pa), critical tangential stress (2.90 × 10⁷ Pa), and the contact radius (1.60 mm). The model’s accuracy was validated through integrated discrete element simulations and prototype testing. The equipment achieves an exceptionally low mussel damage rate of only 1.2%, effectively meeting the operational requirements for mussel unloading. This study provides both theoretical foundations and practical insights for the design of mechanized mussel unloading systems in China. Full article

A rifted margin can be regarded, in the first place, as a crustal thinning taper framed by “box-shaped” continental and oceanic crusts whose top basement and Moho are parallel. Attempts to understand the relationship between lithosphere extension, crustal thinning and strain localization have been addressed in part by characterizing and modeling rift modes. However, a weakness of models stems from their using generalized physical parameters and initial conditions, while each system is unique in terms of its geological complexity. In this study, we develop a new approach to investigate the relation between crustal shape, the nature of the top basement and the accommodation space to reveal the link between extension, strain localization and crustal thinning in the northern South China Sea (N-SCS). Our results show the following: (1) box-shaped crusts may indicate no or minor extension, or extension compensated by crustal flow and/or magmatic additions; (2) crustal thinning and strain localization occurred through extensional detachment faults coevally during the rifting of the N-SCS; (3) strain localization was triggered or enhanced by magmatic weakening, and the weak crustal rheology at the onset of the rifting favored the formation of detachment faults; and (4) the inherited composition of the crust (magmatic rocks in the arc and meta-sediments in the forearc) controls the distribution of crustal thinning. We propose that the different initial conditions, changes in extension rates and the presence/absence of subduction dynamics account for the different rift evolutions observed in the SCS and Atlantic-type rift systems. Full article

Reverse electrodialysis (RED) enables the efficient conversion of the chemical potential difference between seawater and freshwater into electricity while simultaneously facilitating hydrogen production for integrated energy utilization. Nevertheless, the widespread deployment of RED remains constrained by the reliance on ruthenium–iridium-coated electrodes, which are expensive and resource-limited. This study proposes the adoption of titanium-based redox electrodes as a replacement for traditional precious metal electrodes and employs a novel spike structure to accelerate hydrogen bubble detachment. The electrochemical performance of titanium electrodes in an RED hydrogen production system was systematically evaluated experimentally. The influences of several parameters on the RED system performance were systematically examined under these operating conditions, including the ruthenium–iridium catalytic layer, operating temperature (15 to 45 °C), electrode rinse solution (ERS) concentration (0.1 to 0.7 M), and flow rate (50 to 130 mL·min⁻¹). Experimental results demonstrate that optimized titanium redox electrodes maintain high electrocatalytic activity while significantly reducing system costs. Under optimal conditions, the hydrogen yield of the Ti redox electrode reached 89.7% of that achieved with the mesh titanium plate coated oxide iridium and oxide ruthenium as electrodes, while the electrode cost was reduced by more than 60%. This is also one of the cost-cutting solutions adopted by RED for its development. Full article

L. monocytogenes is the causative agent of human listeriosis, a deadly disease with fatality rates up to 20%. L. monocytogenes has the ability to grow under harsh environmental conditions. It can form biofilms in food industries, making it capable of persisting in facilities. Given this scenario, it is of utmost importance to rapidly detect this bacterium not only in foods but also on food-contact surfaces. For the successful outcome of any given detection technology, it is imperative to properly process the samples. In the present work, PBS, LPT, and LPT-Pronase were compared to determine which one could provide better results in DNA-based detection. Additionally, the effect of a short TSB pre-enrichment was assessed. To better mimic a real scenario, L. monocytogenes monospecies and multispecies biofilms were analyzed. It was observed that supplementing LPT with pronase, a protein-degrading enzyme, could better detach the biofilm, which achieved a 0.5 cycle reduction compared to the other broths, and the pre-enrichment reduced the real-time PCR by ~2 cycles. The samples were analyzed by real-time PCR and colorimetric LAMP, and the same results were obtained with both techniques regardless of the concentration of L. monocytogenes present in the biofilm; the initial concentration was 1.8 log CFU/cm² 15 min after the pre-enrichment. The results were confirmed by real-time PCR, which demonstrated the applicability of the methodology to be applied in decentralized setups, such as food-processing facilities, with minimal laboratory infrastructure. Full article

To accommodate the downward-pressing seedling-picking method, this study designed a combination-type plug tray composed of a bottomless plug tray paired with a seedling base plate. The effects of peat ratio, substrate filling ratio, and nutrient solution concentration on pepper plug-seedling performance were evaluated through cultivation experiments and physical–mechanical tests, using seedling vigor index, root biomass, compressive yield strength, and substrate fragmentation rate as assessment indicators. In addition, soil-block detachment force tests were conducted to examine the influence of substrate moisture content on seedling-picking resistance. The results showed that seedling vigor index, root biomass, and compressive yield strength first increased and then declined as the levels of the three factors increased. Higher peat ratios and greater nutrient solution concentrations significantly reduced substrate fragmentation rates. The detachment force of the soil block was highly sensitive to moisture content, with an average value of 6.8 N when the substrate moisture content ranged from 65% to 75%. Based on a comprehensive evaluation approach, the optimal cultivation parameters were determined to be a substrate ratio of 3:1:1, a compaction coefficient of 1.2, and a nutrient solution concentration of 4.0‰. These results provide technical support for optimizing combined plug-seedling cultivation and for the design and parameter determination of seedling-picking mechanisms. Full article

Background/Objectives: Bioresorbable polylactic acid (PLA) matrices have shown promise in supporting wound healing through their biocompatibility, tissue integration, and potential involvement in immune regulatory mechanisms. This study aimed to analyze the clinical performance of a PLA-based matrix in the treatment of chronic wounds under real-world conditions in a single-center setting. Methods: This retrospective study included patients with chronic wounds treated with the polylactic acid matrix at Evangelisches Waldkrankenhaus Spandau between February 2023 and February 2025. Wounds were surgically debrided in the operating room prior to matrix application. The matrix remained in place until resorption or detachment, with reapplications occurring at a mean interval of approximately 14 days. Data was anonymized and analyzed descriptively. Results: A total of 14 patients with 16 chronic wounds were treated in this study. The mean patient age was 76.1 years. The most common underlying causes were ischemia and trauma, with an average wound size of 23.6 cm². Complete wound closure was achieved in 15 out of 16 cases (93.8%), with a mean time to complete wound closure of 72.9 days. The average duration of hospitalization was 24.8 days. Conclusions: The polylactic acid matrix demonstrated a high rate of short-term wound closure in a heterogeneous cohort of chronic wounds, with a mean time to closure of 73 days and no requirement for skin grafting. Further prospective studies with standardized long-term follow-up are warranted. Full article

(1) Background: Globe-sparing radiotherapy is widely utilised in the treatment of uveal melanoma, but often results in complications requiring vitreoretinal intervention. The outcomes of secondary vitrectomy remain unclear. A multidisciplinary approach involving vitreoretinal and ocular oncology specialists is essential to managing complications. (2) Methods: We reviewed 1794 patients treated with radiotherapy for uveal melanoma between 2012 and 2022. In total, 70 patients underwent secondary vitrectomy after primary radiotherapy treatment. The outcomes included overall tumour control and visual outcome. (3) Results: Complications requiring vitrectomy were more common after proton-beam radiotherapy than plaque brachytherapy (5.4% versus 3.0%). Common indications included vitreous haemorrhage (39%) and retinal detachment/toxic tumour syndrome (31%). The affected tumours were larger, more often ciliary body in origin, and associated with a worse prognosis. Vitrectomy patients had higher rates of enucleation (9% versus 3%), metastasis (16% versus 6%), and visual decline (average 0.60 LogMAR), with limited visual improvement (≥3-line gain in 13%). Proton-beam patients had worse outcomes than plaque brachytherapy patients. (4) Conclusions: Vitreoretinal complications after uveal melanoma radiotherapy are rare, but timely treatment by those with experience may enable patients to keep their eye in situations where enucleation would be the only alternative. Patients and clinicians must understand the risks of complications to make informed decisions about treatment plans, with vitreoretinal surgeons and ocular oncologists key to outcomes. Full article

Background: Umbilical venous catheter (UVC) placement is common in neonates but carries risks of migration and infection. This study evaluates safety of a novel fixation technique using the umbilical cord clamp after a side-entry insertion. Methods: A retrospective analysis of 264 neonates was conducted at a tertiary center in order to assess safety of the novel UVC fixation method. The new technique involved side-entry catheter insertion without severing the cord, secured to the clamp with a sterile patch. Catheter tip position was confirmed and monitored every 24 h via ultrasound. Results: Catheter migration occurred in 18.9% of cases, mostly inward into the right atrium which was managed by repositioning. Migration into the ductus venosus requiring removal occurred in 0.7% of cases and unscheduled removal due to stump detachment in 1.5%. No central line-associated bloodstream infections (CLABSIs) were observed. Conclusions: The umbilical cord clamp method is a safe, single-operator alternative for UVC fixation. This technique had a low rate of catheter migration, did not increase the risk of infection, and was cost-effective and simple. Full article

To address the challenges of inefficient Camellia oleifera fruits harvesting in hilly and mountainous regions due to the difficulty of using large machinery, a handheld vibration harvesting device for Camellia oleifera fruits was designed. Based on the vibration-induced detachment process of Camellia oleifera fruits, a single-pendulum dynamic model of the “fruit-branch” system was established and solved to calculate the tangential acceleration required for fruit detachment. The key factors influencing harvesting efficiency were identified as vibration frequency, amplitude, height, and duration. Using ANSYS, modal response and harmonic response analyses were conducted on a 3D model of the Camellia oleifera tree to determine the operational parameters ensuring branch acceleration meets the fruit detachment. Furthermore, a rigid-flexible coupling simulation system integrating the harvesting device and Camellia oleifera tree was developed on the ADAMS. This analysis revealed the variation patterns of branch acceleration with respect to vibration frequency and clamping height, thereby validating the rationality of the dynamic model and the feasibility of the device. Finally, an orthogonal experiment was designed using Design-Expert 13, and multi-objective optimization analysis was performed on the device’s working parameters based on the experimental data. The aforementioned research identified the optimal working parameter combination and actual harvesting performance of the handheld vibration harvesting device: when the vibration frequency is 14 Hz, vibration height is 980 mm, and vibration duration is 13 s, the fruit picking rate reaches 95.22%. The harvesting efficiency of this device is significantly higher than manual picking methods, fully meeting the requirements for efficient Camellia oleifera fruit harvesting. Full article

This research examines the persistent shoreline erosion along the Damietta coast and the problem of sediment buildup in the navigation channel of Damietta Port, both of which pose major obstacles to navigation efficiency and coastal balance. To address these issues, this study uses the LITPACK numerical model to forecast shoreline evolution along the Damietta coast over the next 20 years; the coast is divided into two sections of 3.3 km each. Considering both planned and existing coastal constructions, two realistic alternatives were proposed: extending the existing detached breakwaters by adding two additional offshore breakwaters west of the current field and the implementation of reclamation between the Y-groins, accompanied by a new protruding seawall. The Coastal Modeling System (CMS) was then used to perform a two-dimensional simulation in order to examine sediment transport and hydrodynamic behavior in the port region. This phase concentrated on examining the effects of sedimentation rates following the most recent port development plan, which included building a massive western jetty (5560 m long) and a new navigation channel with a depth of 9 m to service the dirty ballast terminal. In comparison to the benchmark case, the simulation results showed a 93% decrease in sedimentation rates within the navigation channel. The study’s final phase evaluated the impact of changing the crest levels of the current detached breakwaters along the Ras El-Bar coastline on reducing coastal erosion. The study’s conclusions promote the creation of effective and sustainable coastal protection plans in the Damietta area by providing detailed information for future coastal zone management and planning. Full article

Manual wheelchair users with spinal cord injury (SCI) often experience upper-limb strain and pain due to repetitive propulsion. A detachable dual-propulsion add-on device has been developed to mitigate this issue by offering an alternative propulsion mechanism, but its user acceptability and practical benefits must be rigorously evaluated. This study will implement a structured mixed-methods usability assessment of the new device with 30 adult wheelchair users with SCI. The evaluation will combine quantitative surveys, objective task-based performance metrics, and qualitative interviews to capture a comprehensive picture of usability. We will conduct a single-arm mixed-methods protocol using a device-specific 45-item usability questionnaire and semi-structured interviews, followed by convergent triangulation to integrate quantitative scores and qualitative themes. Participants will use the dual-propulsion device in realistic scenarios and then complete a 45-item questionnaire covering effectiveness, efficiency, safety, comfort, and psychosocial satisfaction. In addition, semi-structured interviews will explore users’ experiences, perceived benefits, challenges, and suggestions. During a standardized mobility task course (doorway navigation, ramp ascent, threshold crossing, and 50 m level propulsion), objective performance indicators—including task completion time, task success/error rate, number of lever strokes, and self-selected speed—will be recorded as secondary usability outcomes. The use of both a standardized questionnaire and in-depth interviews will ensure both broad and nuanced assessment of the device’s usability. Data from the survey will be analyzed for usability scores across multiple domains, while interview transcripts will undergo thematic analysis to enrich and validate the quantitative findings. This protocol is expected to provide robust evidence of the device’s usability, inform iterative improvements in its design, and highlight the importance of structured usability evaluations for assistive technologies. Full article

In recent years, microneedles (MNs) have emerged as a novel transdermal drug delivery technology, offering advantages such as avoidance of the first-pass effect, pain-free and minimally invasive administration, and convenient application. However, conventional MNs still face challenges, including slow detachment of MN tips from the base substrate and limited transdermal efficiency. This review systematically summarizes recent advances in MNs-mediated gas delivery for rapid separation, enhanced drug penetration, and combined therapy. The discussion encompasses the benefits and limitations of MNs and recent developments in MN-facilitated gas delivery to accelerate separation rate and improve delivery efficiency. By analyzing the therapeutic roles of various gases (e.g., H₂, O₂, NO, H₂S, CO, CO₂) and their synergistic potential when combined with MNs, this review also provides insights and references for the further application of gas-assisted MN systems for combined therapy in various disease treatments. Full article