Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Article Types

Countries / Regions

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Search Results (164,490)

Search Parameters:
Keywords = demonstration study

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
21 pages, 3446 KiB  
Article
Targeting the Kynureninase–HDAC6–Complement Axis as a Novel Therapeutic Strategy in Glioblastoma
by Arif Ul Hasan, Sachiko Sato, Mami Obara, Yukiko Kondo and Eiichi Taira
Epigenomes 2025, 9(3), 27; https://doi.org/10.3390/epigenomes9030027 (registering DOI) - 28 Jul 2025
Abstract
Background/Objectives: Glioblastoma (GBM) is an aggressive brain tumor known for its profound heterogeneity and treatment resistance. Dysregulated complement signaling and epigenetic alterations have been implicated in GBM progression. This study identifies kynureninase (KYNU), a key enzyme in the kynurenine pathway, as a novel [...] Read more.
Background/Objectives: Glioblastoma (GBM) is an aggressive brain tumor known for its profound heterogeneity and treatment resistance. Dysregulated complement signaling and epigenetic alterations have been implicated in GBM progression. This study identifies kynureninase (KYNU), a key enzyme in the kynurenine pathway, as a novel regulator of complement components and investigates its interaction with histone deacetylase 6 (HDAC6) in the context of therapeutic targeting. Methods: KYNU expression, and its association with complement signaling in GBM, were analyzed using publicly available datasets (TCGA, GTEx, HPA). Pathway enrichment was performed via LinkedOmics. In vitro studies in GBM cell lines (U87, U251, T98G) assessed the effects of KYNU silencing and treatment with an HDAC6 inhibitor (tubastatin) and a BET inhibitor (apabetalone) on gene expression and cell viability. Results: Bioinformatic analyses revealed significant overexpression of KYNU in GBM tissues compared to normal brain tissue. KYNU expression was positively associated with genes involved in complement and coagulation cascades. In vitro experiments demonstrated that KYNU silencing reduced the expression of C3, C3AR1, and C5AR1 and suppressed GBM cell viability. Treatment with tubastatin, while reducing viability, paradoxically upregulated complement genes, suggesting potential limitations in therapeutic efficacy. However, this effect was mitigated by KYNU knockdown. Combined treatment with apabetalone and tubastatin effectively suppressed KYNU expression and enhanced cytotoxicity, particularly in cells with high complement expression. Conclusions: Our findings establish the KYNU–HDAC6–complement axis as a critical regulatory pathway in GBM. Targeting KYNU-mediated complement activation through combined epigenetic approaches—such as HDAC6 and BET inhibition—represents a promising strategy to overcome complement-driven resistance in GBM therapy. Full article
Show Figures

Figure 1

9 pages, 479 KiB  
Review
Photobiomodulation as a Hypothetical Strategy to Reverse Botulinum Toxin Effects: Exploring the Neuroregenerative Mechanisms and Translational Potential
by Rodrigo Álvaro Brandão Lopes-Martins, Francisco Gonzalez-Lima, Sérgio Gomes da Silva, Patrícia Sardinha Leonardo, Cristiane Soncino, Roberto Fernandes Pacheco, Carolina Lúcia de Oliveira e Oliveira and Fabrizio dos Santos Cardoso
Life 2025, 15(8), 1206; https://doi.org/10.3390/life15081206 (registering DOI) - 28 Jul 2025
Abstract
Background: Botulinum toxin type A (BoNT/A) is widely used in both clinical and aesthetic settings to induce temporary neuromuscular paralysis by inhibiting acetylcholine release. Although generally regarded as safe and effective, complications such as iatrogenic ptosis or facial asymmetry may occur and persist [...] Read more.
Background: Botulinum toxin type A (BoNT/A) is widely used in both clinical and aesthetic settings to induce temporary neuromuscular paralysis by inhibiting acetylcholine release. Although generally regarded as safe and effective, complications such as iatrogenic ptosis or facial asymmetry may occur and persist for several weeks or even months, with no standardized method currently available to accelerate recovery. Objective: This article explores the hypothesis that photobiomodulation (PBM)—a non-invasive modality recognized for its neuroregenerative potential—may facilitate the reversal of BoNT/A-induced neuromuscular blockade. Discussion: PBM enhances mitochondrial activity by stimulating cytochrome c oxidase in nerve and muscle tissues, thereby increasing ATP production and modulating intracellular signaling pathways associated with neuroplasticity, cell survival, and synaptogenesis. Preclinical studies have demonstrated that PBM can upregulate neurotrophic factors (e.g., BDNF, NGF), enhance SNAP-25 expression, and promote structural remodeling of neurons in both young and aged brains. These mechanisms are biologically consistent with the regenerative processes required for recovery from BoNT/A-induced effects. While controlled clinical trials for this specific application are currently lacking, anecdotal clinical reports suggest that PBM may accelerate functional recovery in cases of BoNT/A-related complications. Conclusions: Although this approach has not yet been tested in clinical trials, we propose that photobiomodulation may hypothetically serve as a supportive strategy to promote neuromuscular recovery in patients experiencing adverse effects from BoNT/A. This hypothesis is grounded in robust preclinical evidence but requires validation through translational and clinical research. Full article
(This article belongs to the Section Physiology and Pathology)
23 pages, 3051 KiB  
Article
Digital Inequality and Smart Inclusion: A Socio-Spatial Perspective from the Region of Xanthi, Greece
by Kyriaki Kourtidou, Yannis Frangopoulos, Asimenia Salepaki and Dimitris Kourkouridis
Smart Cities 2025, 8(4), 123; https://doi.org/10.3390/smartcities8040123 - 28 Jul 2025
Abstract
This study explores digital inequality as a socio-spatial phenomenon within the context of smart inclusion, focusing on the Regional Unit of Xanthi, Greece—a region marked by ethno-cultural diversity and pronounced urban–rural contrasts. Using a mixed-methods design, this research integrates secondary quantitative data with [...] Read more.
This study explores digital inequality as a socio-spatial phenomenon within the context of smart inclusion, focusing on the Regional Unit of Xanthi, Greece—a region marked by ethno-cultural diversity and pronounced urban–rural contrasts. Using a mixed-methods design, this research integrates secondary quantitative data with qualitative insights from semi-structured interviews, aiming to uncover how spatial, demographic, and cultural variables shape digital engagement. Geographic Information System (GIS) tools are employed to map disparities in internet access and ICT infrastructure, revealing significant gaps linked to geography, education, and economic status. The findings demonstrate that digital inequality is particularly acute in rural, minority, and economically marginalized communities, where limited infrastructure intersects with low digital literacy and socio-economic disadvantage. Interview data further illuminate how residents navigate exclusion, emphasizing generational divides, perceptions of technology, and place-based constraints. By bridging spatial analysis with lived experience, this study advances the conceptualization of digitally inclusive smart regions. It offers policy-relevant insights into how territorial inequality undermines the goals of smart development and proposes context-sensitive interventions to promote equitable digital participation. The case of Xanthi underscores the importance of integrating spatial justice into smart city and regional planning agendas. Full article
21 pages, 6301 KiB  
Article
HGF Overexpression in Mesenchymal Stromal Cell-Based Cell Sheets Enhances Autophagy-Dependent Cytoprotection and Proliferation to Guard the Epicardial Mesothelium
by Konstantin Dergilev, Irina Beloglazova, Zoya Tsokolaeva, Ekaterina Azimova, Aleria Dolgodvorova, Yulia Goltseva, Maria Boldyreva, Mikhail Menshikov, Dmitry Penkov and Yelena Parfyonova
Int. J. Mol. Sci. 2025, 26(15), 7298; https://doi.org/10.3390/ijms26157298 - 28 Jul 2025
Abstract
Epicardial mesothelial cells (EMCs), which form the epicardium, play a crucial role in cardiac homeostasis and repair. Upon damage, EMCs reactivate embryonic development programs, contributing to wound healing, progenitor cell amplification, and regulation of lymphangiogenesis, angiogenesis, and fibrosis. However, the mechanisms governing EMC [...] Read more.
Epicardial mesothelial cells (EMCs), which form the epicardium, play a crucial role in cardiac homeostasis and repair. Upon damage, EMCs reactivate embryonic development programs, contributing to wound healing, progenitor cell amplification, and regulation of lymphangiogenesis, angiogenesis, and fibrosis. However, the mechanisms governing EMC activation and subsequent regulation remain poorly understood. We hypothesized that hepatocyte growth factor (HGF), a pleiotropic regulator of various cellular functions, could modulate EMC activity. To verify this hypothesis, we developed HGF-overexpressing mesenchymal stromal cell sheets (HGF-MSC CSs) and evaluated their effects on EMCs in vitro and in vivo. This study has revealed, for the first time, that EMCs express the c-Met (HGF receptor) on their surface and that both recombinant HGF and HGF-MSC CSs secretome cause c-Met phosphorylation, triggering downstream intracellular signaling. Our findings demonstrate that the HGF-MSC CSs secretome promotes cell survival under hypoxic conditions by modulating the level of autophagy. At the same time, HGF-MSC CSs stimulate EMC proliferation, promoting their amplification in the damage zone. These data demonstrate that HGF-MSC CSs can be considered a promising regulator of epicardial cell activity involved in heart repair after ischemic damage. Full article
37 pages, 3086 KiB  
Article
Conformal On-Body Antenna System Integrated with Deep Learning for Non-Invasive Breast Cancer Detection
by Marwa H. Sharaf, Manuel Arrebola, Khalid F. A. Hussein, Asmaa E. Farahat and Álvaro F. Vaquero
Sensors 2025, 25(15), 4670; https://doi.org/10.3390/s25154670 - 28 Jul 2025
Abstract
Breast cancer detection through non-invasive and accurate techniques remains a critical challenge in medical diagnostics. This study introduces a deep learning-based framework that leverages a microwave radar system equipped with an arc-shaped array of six antennas to estimate key tumor parameters, including position, [...] Read more.
Breast cancer detection through non-invasive and accurate techniques remains a critical challenge in medical diagnostics. This study introduces a deep learning-based framework that leverages a microwave radar system equipped with an arc-shaped array of six antennas to estimate key tumor parameters, including position, size, and depth. This research begins with the evolutionary design of an ultra-wideband octagram ring patch antenna optimized for enhanced tumor detection sensitivity in directional near-field coupling scenarios. The antenna is fabricated and experimentally evaluated, with its performance validated through S-parameter measurements, far-field radiation characterization, and efficiency analysis to ensure effective signal propagation and interaction with breast tissue. Specific Absorption Rate (SAR) distributions within breast tissues are comprehensively assessed, and power adjustment strategies are implemented to comply with electromagnetic exposure safety limits. The dataset for the deep learning model comprises simulated self and mutual S-parameters capturing tumor-induced variations over a broad frequency spectrum. A core innovation of this work is the development of the Attention-Based Feature Separation (ABFS) model, which dynamically identifies optimal frequency sub-bands and disentangles discriminative features tailored to each tumor parameter. A multi-branch neural network processes these features to achieve precise tumor localization and size estimation. Compared to conventional attention mechanisms, the proposed ABFS architecture demonstrates superior prediction accuracy and interpretability. The proposed approach achieves high estimation accuracy and computational efficiency in simulation studies, underscoring the promise of integrating deep learning with conformal microwave imaging for safe, effective, and non-invasive breast cancer detection. Full article
15 pages, 876 KiB  
Article
Rational Design, Synthesis and In Vitro Activity of Diastereomeric Cis-/Trans-3-Substituted-3,4-Dihydroisocoumarin-4-Carboxylic Acids as Potential Carnitine Acetyltransferase Inhibitors
by Savina Stoyanova and Milen G. Bogdanov
Molecules 2025, 30(15), 3159; https://doi.org/10.3390/molecules30153159 - 28 Jul 2025
Abstract
This study explores a series of 3,4-dihydroisocoumarins as potential inhibitors of fatty acid oxidation through rational design, synthesis and in vitro evaluation. The compounds studied were designed as structural analogs of the natural substrates of carnitine acetyltransferase (CAT) and other enzymes in the [...] Read more.
This study explores a series of 3,4-dihydroisocoumarins as potential inhibitors of fatty acid oxidation through rational design, synthesis and in vitro evaluation. The compounds studied were designed as structural analogs of the natural substrates of carnitine acetyltransferase (CAT) and other enzymes in the carnitine transferase family, which play a crucial role in fatty acid metabolism. Comparative in vitro analyses revealed that the presence of an alkyl substituent at position 3 of the heterocyclic core, along with its chain length, significantly influences inhibitory activity, yielding IC50 values in the micromolar range. Kinetic studies of one of the most potent compounds—cis- and trans-3-decyl-6,7-dimethoxy-3,4-dihydroisocoumarin-4-carboxylic acids—demonstrated mixed inhibition of CAT, with Ki values of 130 μM and 380 μM, respectively. These findings underscore the therapeutic potential of the compounds under investigation in modulating fatty acid catabolism, with possible applications in treating metabolic disorders. Full article
13 pages, 1060 KiB  
Article
Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows
by Liani Coronado, Àlex Cobos, Adriana Muñoz-Aguilera, Sara Puente-Marin, Gemma Guevara, Cristina Riquelme, Saray Heredia, Manuel V. Borca and Llilianne Ganges
Vaccines 2025, 13(8), 803; https://doi.org/10.3390/vaccines13080803 - 28 Jul 2025
Abstract
Background/Objectives: Classical swine fever (CSF) continues to challenge global eradication efforts, particularly in endemic regions, where pregnant sows face heightened risks of vertical transmission following exposure to CSFV. Methods: This study evaluates the early protective efficacy of FlagT4G, a novel live attenuated DIVA-compatible [...] Read more.
Background/Objectives: Classical swine fever (CSF) continues to challenge global eradication efforts, particularly in endemic regions, where pregnant sows face heightened risks of vertical transmission following exposure to CSFV. Methods: This study evaluates the early protective efficacy of FlagT4G, a novel live attenuated DIVA-compatible vaccine. Pregnant sows were vaccinated at mid-gestation and challenged 14 days later with a highly virulent CSFV strain. Results: FlagT4G conferred complete clinical protection, preventing both maternal viremia and transplacental transmission. No CSFV RNA, specific antibodies, or IFN-α were detected in fetal samples from vaccinated animals. In contrast, unvaccinated sows exhibited clinical signs, high viral loads, and widespread fetal infection. Interestingly, early protection was observed even in the absence of strong humoral responses in some vaccinated sows, suggesting a potential role for innate or T-cell-mediated immunity in conferring rapid protection. Conclusions: The demonstrated efficacy of FlagT4G within two weeks of vaccination underscores its feasibility for integration into emergency vaccination programs. Its DIVA compatibility and ability to induce early fetal protection against highly virulent CSFV strains position it as a promising tool for CSF control and eradication strategies. Full article
(This article belongs to the Special Issue Vaccines for Porcine Viruses)
18 pages, 7058 KiB  
Article
Does Urban Economic Development Increase Sewage Discharge Intensity? A Case Study of 288 Cities in China
by Xiaoli Yue, Yingmei Wu, Yang Wang, Wenlu Li, Yufei Wang, Guiquan Sun and Hong’ou Zhang
Water 2025, 17(15), 2251; https://doi.org/10.3390/w17152251 - 28 Jul 2025
Abstract
Accelerated urbanization and intensified urban development globally lead to increased sewage discharge, challenging environmental protection. Therefore, exploring the correlation mechanism between the economic development level (EDL) and sewage discharge intensity (SDI) is crucial for sustainable development. This study uses panel data from 288 [...] Read more.
Accelerated urbanization and intensified urban development globally lead to increased sewage discharge, challenging environmental protection. Therefore, exploring the correlation mechanism between the economic development level (EDL) and sewage discharge intensity (SDI) is crucial for sustainable development. This study uses panel data from 288 Chinese cities between 2003 and 2021, employs spatial analysis techniques to uncover the spatiotemporal evolution characteristics of SDI, and investigates the influence of economic development on this intensity using spatial panel models. The results reveal that (1) while the spatial distribution of SDI in China generally exhibits a downward trend, changes in the Northeast region are relatively modest, with SDI remaining higher than in other regions. Global autocorrelation analysis further indicates significant spatial agglomeration and positive correlation effects in urban SDI. (2) Economic development exerts a notable inhibitory effect on SDI, with a 0.570% decrease for every 1% rise in GDP per capita, thus demonstrating a significant spatial spillover effect. (3) For megacities, large cities, and small and medium-sized cities, EDLs have significant negative spatial spillover effects on SDI, with a more pronounced impact on large cities. This study provides a theoretical foundation for sewage management and empirical support for environmental policies, crucial for sustainable urban development. Full article
(This article belongs to the Section Urban Water Management)
Show Figures

Figure 1

22 pages, 5331 KiB  
Article
Beyond a Single Story: The Complex and Varied Patterns of Park Accessibility Across China’s Emerging Cities
by Mengqi Liu and Toru Terada
Land 2025, 14(8), 1552; https://doi.org/10.3390/land14081552 - 28 Jul 2025
Abstract
China’s rapid urbanization has driven tremendous socioeconomic development while posing new forms of social–spatial inequalities that challenge environmental sustainability and spatial justice. This study investigates urban park-accessibility patterns across 10 s-tier provincial capital cities in China, examining how these patterns relate to housing-price [...] Read more.
China’s rapid urbanization has driven tremendous socioeconomic development while posing new forms of social–spatial inequalities that challenge environmental sustainability and spatial justice. This study investigates urban park-accessibility patterns across 10 s-tier provincial capital cities in China, examining how these patterns relate to housing-price dynamics to reveal diverse manifestations of social–spatial (in)justice. Using comprehensive spatial analysis grounded in distributive justice principles, we measure park accessibility through multiple metrics: distance to the nearest park, park size, and the number of parks within a 15 min walk from residential communities. Our findings reveal significant variation in park accessibility across these cities, with distinctive patterns emerging in the relationship between housing prices and park access that reflect different forms of social–spatial exclusion and inclusion. While most cities demonstrate an unbalanced spatial distribution of parks, they exhibit different forms of this disparity. Some cities show consistent park access across housing-price categories, while others display correlations between high housing prices and superior park accessibility. We argue that these divergent patterns reflect each city’s unique combination of economic development trajectory, politically strategic positioning within national urban hierarchies, and geographical constraints. Through this comparative analysis of second-tier cities, this study contributes to broader understandings of social–spatial (in)justice and urban environmental inequalities within China’s urbanization process, highlighting the need for place-specific approaches to achieving equitable access to urban amenities. Full article
(This article belongs to the Special Issue Spatial Justice in Urban Planning (Second Edition))
20 pages, 1619 KiB  
Article
Adsorption of Pb2+ and Cd2+ from Aqueous Solutions by Porous Carbon Foam Derived from Biomass Phenolic Resin
by Jianwei Ling, Yu Gao, Ruiling Wang, Shiyu Lu, Xuemei Li, Shouqing Liu and Jianxiang Liu
Int. J. Mol. Sci. 2025, 26(15), 7302; https://doi.org/10.3390/ijms26157302 - 28 Jul 2025
Abstract
Due to its lightweight and superior adsorption properties, carbon foam is frequently employed for the removal of heavy metal pollutants from aqueous solutions. In this study, a novel modified carbon foam (M-CF) was successfully synthesized for the effective removal of Pb2+ and [...] Read more.
Due to its lightweight and superior adsorption properties, carbon foam is frequently employed for the removal of heavy metal pollutants from aqueous solutions. In this study, a novel modified carbon foam (M-CF) was successfully synthesized for the effective removal of Pb2+ and Cd2+ from water. The synthesis involved partially substituting phenol with the liquefaction product of bamboo powder, followed by modification with a silane coupling agent (KH560) and foaming with n-hexane-loaded activated carbon (H/AC). The prepared carbon foam was comprehensively characterized, and its adsorption performance and mechanism for Pb2+ and Cd2+ in aqueous solution were investigated. The results showed that M-CF possessed a uniform and well-developed spherical pore structure and demonstrated excellent removal capacity for Cd2+ and Pb2+. The adsorption process conformed to the Sips isotherm model and the pseudo-second-order kinetic equation, with maximum adsorption capacities of 22.15 mg·g−1 and 61.59 mg·g−1 for Cd2+ and Pb2+, respectively. Mechanistic analysis revealed that the removal of Cd2+ and Pb2+ was a result of the synergistic effect of physisorption and chemisorption, accompanied by complexation. Furthermore, precipitates formed during the adsorption process were found to be mainly composed of hydroxides, carbonates, and PbS. This research demonstrates the efficacy of carbon foam prepared from bamboo powder waste as a partial phenol substitute for the efficient removal of Pb2+ and Cd2+ from water, thus expanding the preparation pathways for novel heavy metal adsorption materials. Full article
20 pages, 3073 KiB  
Article
Fusion of airborne, SLAM-based, and iPhone LiDAR for Accurate Forest Road Mapping in Harvesting Areas
by Evangelia Siafali, Vasilis Polychronos and Petros A. Tsioras
Land 2025, 14(8), 1553; https://doi.org/10.3390/land14081553 - 28 Jul 2025
Abstract
This study examined the integraftion of airborne Light Detection and Ranging (LiDAR), Simultaneous Localization and Mapping (SLAM)-based handheld LiDAR, and iPhone LiDAR to inspect forest road networks following forest operations. The goal is to overcome the challenges posed by dense canopy cover and [...] Read more.
This study examined the integraftion of airborne Light Detection and Ranging (LiDAR), Simultaneous Localization and Mapping (SLAM)-based handheld LiDAR, and iPhone LiDAR to inspect forest road networks following forest operations. The goal is to overcome the challenges posed by dense canopy cover and ensure accurate and efficient data collection and mapping. Airborne data were collected using the DJI Matrice 300 RTK UAV equipped with a Zenmuse L2 LiDAR sensor, which achieved a high point density of 285 points/m2 at an altitude of 80 m. Ground-level data were collected using the BLK2GO handheld laser scanner (HPLS) with SLAM methods (LiDAR SLAM, Visual SLAM, Inertial Measurement Unit) and the iPhone 13 Pro Max LiDAR. Data processing included generating DEMs, DSMs, and True Digital Orthophotos (TDOMs) via DJI Terra, LiDAR360 V8, and Cyclone REGISTER 360 PLUS, with additional processing and merging using CloudCompare V2 and ArcGIS Pro 3.4.0. The pairwise comparison analysis between ALS data and each alternative method revealed notable differences in elevation, highlighting discrepancies between methods. ALS + iPhone demonstrated the smallest deviation from ALS (MAE = 0.011, RMSE = 0.011, RE = 0.003%) and HPLS the larger deviation from ALS (MAE = 0.507, RMSE = 0542, RE = 0.123%). The findings highlight the potential of fusing point clouds from diverse platforms to enhance forest road mapping accuracy. However, the selection of technology should consider trade-offs among accuracy, cost, and operational constraints. Mobile LiDAR solutions, particularly the iPhone, offer promising low-cost alternatives for certain applications. Future research should explore real-time fusion workflows and strategies to improve the cost-effectiveness and scalability of multisensor approaches for forest road monitoring. Full article
34 pages, 27180 KiB  
Article
Lifetime Variations in Prolactin Expression in the Hippocampus and Dentate Gyrus of the Rat
by Marta Carretero-Hernández, Elisa Herráez, Leonardo Catalano-Iniesta, David Hernández-González, David Díez-Castro, Ana E. Rodríguez-Vicente, Josefa García-Barrado, Teresa Vicente-García, Miguel Robles-García, Enrique J. Blanco and José Carretero
Int. J. Mol. Sci. 2025, 26(15), 7299; https://doi.org/10.3390/ijms26157299 - 28 Jul 2025
Abstract
Prolactin is a hormone with demonstrated roles in the brain, including neurogenesis, neuroprotection, learning, stress response or memory consolidation. To determine the prolactin expression in the rat hippocampus during aging and to resolve some controversies related to the presence of prolactin in the [...] Read more.
Prolactin is a hormone with demonstrated roles in the brain, including neurogenesis, neuroprotection, learning, stress response or memory consolidation. To determine the prolactin expression in the rat hippocampus during aging and to resolve some controversies related to the presence of prolactin in the hippocampus, the aim of this study was to analyze whether changes occur in the expression of prolactin during different stages of life. To determine this, we designed an experimental study in which we analyzed the expression and location of prolactin in the rat hippocampus, Ammon’s horn and Dentate Gyrus, during different stages of life (prepubertal, postpubertal, young adult, adult and old) and checked if there are differences related to sex. Overall, the results obtained show that prolactin is present in the rat hippocampus and that prolactin is synthesized, as deduced from the findings obtained via ELISA, immunohistochemistry, qPCR and in situ hybridization. After analyzing the correlation between serum and hippocampal prolactin levels and comparing the amounts of Prl mRNA and the hormone, the results obtained suggest that hippocampal prolactin has a dual origin: local synthesis of the hormone and its passage from the blood. On the other hand, the amounts of prolactin and its mRNA in the hippocampus vary with sex and age, suggesting the existence of age-related sexual dimorphism. The results obtained suggest that hippocampal aging is related to a decrease in the hippocampal prolactin system, which helps to better understand brain aging. Full article
(This article belongs to the Special Issue Understanding Aging in Health and Disease)
Show Figures

Figure 1

20 pages, 1414 KiB  
Systematic Review
Comparison of the Effects of Cold-Water Immersion Applied Alone and Combined Therapy on the Recovery of Muscle Fatigue After Exercise: A Systematic Review and Meta-Analysis
by Junjie Ma, Changfei Guo, Long Luo, Xiaoke Chen, Keying Zhang, Dongxue Liang and Dong Zhang
Life 2025, 15(8), 1205; https://doi.org/10.3390/life15081205 - 28 Jul 2025
Abstract
Cold-water immersion (CWI), as a common recovery method, has been widely used in the field of post-exercise fatigue recovery. However, there is still a lack of comprehensive and systematic scientific evaluation of the combined effects of cold-water immersion combined with other therapies (CWI [...] Read more.
Cold-water immersion (CWI), as a common recovery method, has been widely used in the field of post-exercise fatigue recovery. However, there is still a lack of comprehensive and systematic scientific evaluation of the combined effects of cold-water immersion combined with other therapies (CWI + Other). The aim of this study was to compare the effects of CWI and CWI + Other in post-exercise fatigue recovery and to explore the potential benefits of CWI + Other. We systematically searched PubMed, Embase, Web of Science, Cochrane Library and EBSCO databases to include 24 studies (475 subjects in total) and performed a meta-analysis using standardized mean difference (SMD) and 95% confidence intervals (CIs). The results showed that both CWI + Other (SMD = −0.68, 95% CI: −1.03 to −0.33) and CWI (SMD = −0.37, 95% CI: −0.65 to −0.10) were effective in reducing delayed-onset muscle soreness (DOMS). In subgroup analyses of athletes, both CWI + Other (SMD = −1.13, 95% CI: −1.76 to −0.49) and CWI (SMD = −0.47, 95% CI: −0.87 to −0.08) also demonstrated significant effects. In addition, CWI + Other significantly reduced post-exercise C-reactive protein (CRP) levels (SMD = −0.62, 95% CI: −1.12 to −0.13), and CWI with water temperatures higher than 10 °C also showed a CRP-lowering effect (MD = −0.18, 95% CI: −0.30 to −0.07), suggesting a potential benefit in anti-inflammation. There were no significant differences between the two interventions in the metrics of creatine kinase (CK; CWI: SMD = −0.01, 95% CI: −0.27 to 0.24; CWI + Other: SMD = 0.26, 95% CI: −0.51 to 1.03) or countermovement jump (CMJ; CWI: SMD = 0.22, 95% CI: −0.13 to 0.57; CWI + Other: SMD = 0.07, 95% CI: −0.70 to 0.85). Full article
(This article belongs to the Special Issue Focus on Exercise Physiology and Sports Performance: 2nd Edition)
16 pages, 972 KiB  
Article
Automated Assessment of Word- and Sentence-Level Speech Intelligibility in Developmental Motor Speech Disorders: A Cross-Linguistic Investigation
by Micalle Carl and Michal Icht
Diagnostics 2025, 15(15), 1892; https://doi.org/10.3390/diagnostics15151892 - 28 Jul 2025
Abstract
Background/Objectives: Accurate assessment of speech intelligibility is necessary for individuals with motor speech disorders. Transcription or scaled rating methods by naïve listeners are the most reliable tasks for these purposes; however, they are often resource-intensive and time-consuming within clinical contexts. Automatic speech [...] Read more.
Background/Objectives: Accurate assessment of speech intelligibility is necessary for individuals with motor speech disorders. Transcription or scaled rating methods by naïve listeners are the most reliable tasks for these purposes; however, they are often resource-intensive and time-consuming within clinical contexts. Automatic speech recognition (ASR) systems, which transcribe speech into text, have been increasingly utilized for assessing speech intelligibility. This study investigates the feasibility of using an open-source ASR system to assess speech intelligibility in Hebrew and English speakers with Down syndrome (DS). Methods: Recordings from 65 Hebrew- and English-speaking participants were included: 33 speakers with DS and 32 typically developing (TD) peers. Speech samples (words, sentences) were transcribed using Whisper (OpenAI) and by naïve listeners. The proportion of agreement between ASR transcriptions and those of naïve listeners was compared across speaker groups (TD, DS) and languages (Hebrew, English) for word-level data. Further comparisons for Hebrew speakers were conducted across speaker groups and stimuli (words, sentences). Results: The strength of the correlation between listener and ASR transcription scores varied across languages, and was higher for English (r = 0.98) than for Hebrew (r = 0.81) for speakers with DS. A higher proportion of listener–ASR agreement was demonstrated for TD speakers, as compared to those with DS (0.94 vs. 0.74, respectively), and for English, in comparison to Hebrew speakers (0.91 for English DS speakers vs. 0.74 for Hebrew DS speakers). Listener–ASR agreement for single words was consistently higher than for sentences among Hebrew speakers. Speakers’ intelligibility influenced word-level agreement among Hebrew- but not English-speaking participants with DS. Conclusions: ASR performance for English closely approximated that of naïve listeners, suggesting potential near-future clinical applicability within single-word intelligibility assessment. In contrast, a lower proportion of agreement between human listeners and ASR for Hebrew speech indicates that broader clinical implementation may require further training of ASR models in this language. Full article
(This article belongs to the Special Issue Evaluation and Management of Developmental Disabilities)
13 pages, 1130 KiB  
Article
Feasibility and Preliminary Results of a Standardized Stair Climbing Test to Evaluate Cardiorespiratory Fitness in Children and Adolescents in a Non-Clinical Setting: The “Hand Aufs Herz” Study
by Federico Morassutti Vitale, Jennifer Wieprecht, Maren Baethmann, Delphina Gomes, Anja Tengler, Roxana Riley, Samar Shamas, Marcel Müller, Guido Mandilaras, Simone Katrin Manai, Maria Jaros, Nikolaus Alexander Haas and Meike Schrader
Children 2025, 12(8), 993; https://doi.org/10.3390/children12080993 - 28 Jul 2025
Abstract
Background/Objectives: Cardiorespiratory fitness (CRF) is of great interest in children and adolescents. Due to the limited availability of cardiopulmonary exercise testing, simple and reliable alternatives are needed. A stair climbing test (SCT) for the assessment of CRF developed at the Department of [...] Read more.
Background/Objectives: Cardiorespiratory fitness (CRF) is of great interest in children and adolescents. Due to the limited availability of cardiopulmonary exercise testing, simple and reliable alternatives are needed. A stair climbing test (SCT) for the assessment of CRF developed at the Department of Pediatric Cardiology of the LMU University Hospital in Munich showed a strong correlation with VO2max. The aim of this study is to prove its feasibility in a non-clinical setting and to analyse its results in a larger study population. Methods: During the “Hand aufs Herz” study, a comprehensive cardiovascular examination was carried out on 922 pupils and siblings (13.2 ± 7.8 years) at a high school in Bavaria. The SCT was performed to evaluate CRF: participants had to run up and down a total of four floors (14.8 m) as quickly as possible without skipping steps or holding on to the banister. Absolute time has been normalized over the standard height of 12 m to allow comparisons with different settings. An SCT Index was calculated to adjust results to the different weights of participants and the exact height of the staircase. Results: The SCT proved to be easily feasible and safe in non-clinical contexts. Out of 922 participants, 13 (1.4%) were not able to perform the test, and 3 (0.3%) had to interrupt it following fatigue or stumbling. A total of 827 participants aged from 9 to 17 years (13.1 ± 2.1 years, 45.8% girls) had a mean absolute SCT time of 53.4 ± 6.2 s and 43.3 ± 5.1 s when normalized over 12 m. Conclusions: The SCT represents a simple, cost- and time-saving test that allows a rapid and solid assessment of cardiorespiratory fitness in children and adolescents. We could demonstrate that it is safe and feasible in non-clinical contexts. Its short duration and universal applicability are valuable advantages that could facilitate the establishment of a repetitive cardiovascular screening in the pediatric population, particularly in outpatient departments or settings with low-resource systems. Full article
(This article belongs to the Special Issue Prevention of Cardiovascular Diseases in Children and Adolescents)
Show Figures

Figure 1

Back to TopTop