Search Results (1,597)

Urban wildlife conservation is emerging as a critical component of sustainable city ecosystems. Rather than simply increasing tree abundance or species richness, conservation management should focus on key species. In this research, Xishan Forest Park in Beijing was chosen as a case study. Our aim was to identify keystone taxa critical for avian foraging and nesting during the breeding season. We performed a network analysis linking bird species, their diets, and nest plants. Dietary components were detected using DNA metabarcoding conducted with avian fecal samples. Nest plants were identified via transect surveys. Two indices of the network, degree and weighted mean degree, were calculated to evaluate the importance of the dietary and nest plant species. We identified 13 bird host species from 107 fecal samples and 14 bird species from 107 nest observations. Based on the degree indices, fruit trees Morus and Prunus were detected as key food sources, exhibiting both the highest degree (degree = 9, 9) and weighted mean degree (lnwMD = 5.21, 4.63). Robinia pseudoacacia provided predominant nesting sites, with a predominant degree of 7. A few taxa, such as Styphnolobium japonicum and Rhamnus parvifolia, served dual ecological significance as both essential food sources and nesting substrates. Scrublands, as a unique habitat type, provided nesting sites and food for small-bodied birds. Therefore, targeted management interventions are recommended to sustain or enhance these keystone resource species and to maintain the multi-layered vertical vegetation structure to preserve the diverse habitats of birds. Full article

Peatlands store substantial amounts of carbon (C) in the form of peat, but are increasingly threatened by drought and shrub encroachment under climate warming. However, how peat decomposition and its temperature sensitivity (Q₁₀) vary with depth and plant litter input under these stressors remains poorly understood. We incubated peat from two depths with different degrees of decomposition, either alone or incubated with Sphagnum divinum shoots or Betula ovalifolia leaves, under five temperature levels and two moisture conditions in growth chambers. We found that drought and Betula addition increased CO₂ emissions in both peat layers, while Sphagnum affected only shallow peat. Deep peat alone or with Betula exhibited higher Q₁₀ than pure shallow peat. Drought increased the Q₁₀ of both depths’ peat, but this effect disappeared with fresh litter addition. The CO₂ production rate showed a positive but marginal correlation with microbial biomass carbon, and it displayed a rather similar responsive trend to warming as the microbial metabolism quotient. These results indicate that both deep and dry peat are more sensitive to warming, highlighting the importance of keeping deep peat buried and waterlogged to conserve existing carbon storage. Additionally, they further emphasize the necessity of Sphagnum moss recovery following vascular plant encroachment in restoring carbon sink function in peatlands. Full article

Urochloa brizantha (Brizantha) is cultivated under varying altitudinal and management conditions. Twelve full-sun (monoculture) plots and twelve shaded (silvopastoral) plots were established, proportionally distributed at 170, 503, 661, and 1110 masl. Evaluations were conducted 15, 30, 45, 60, and 75 days after establishment. The conservation and integration of trees in silvopastoral systems reflected a clear anthropogenic influence, evidenced by the preference for species of the Fabaceae family, likely due to their multipurpose nature. Although the altitudinal gradient did not show direct effects on soil properties, intermediate altitudes revealed a significant role of CaCO₃ in enhancing soil fertility. These edaphic conditions at mid-altitudes favored the leaf area development of Brizantha, particularly during the early growth stages, as indicated by significantly larger values (p < 0.05). However, at the harvest stage, no significant differences were observed in physiological or productive traits, nor in foliar chemical components, underscoring the species’ high hardiness and broad adaptation to both soil and altitude conditions. In Brizantha, a significant reduction (p < 0.05) in stomatal size and density was observed under shade in silvopastoral areas, where solar radiation and air temperature decreased, while relative humidity increased. Nonetheless, these microclimatic variations did not lead to significant changes in foliar chemistry, growth variables, or biomass production, suggesting a high degree of adaptive plasticity to microclimatic fluctuations. Foliar ash content exhibited an increasing trend with altitude, indicating greater efficiency of Brizantha in absorbing calcium, phosphorus, and potassium at higher altitudes, possibly linked to more favorable edaphoclimatic conditions for nutrient uptake. Finally, forage quality declined with plant age, as evidenced by reductions in protein, ash, and In Vitro Dry Matter Digestibility (IVDMD), alongside increases in fiber, Neutral Detergent Fiber (NDF), and Acid Detergent Fiber (ADF). These findings support the recommendation of cutting intervals between 30 and 45 days, during which Brizantha displays a more favorable nutritional profile, higher digestibility, and consequently, greater value for animal feeding. Full article

We investigated the dispersal ability of Euphydryas aurinia provincialis in a local-scale analysis within a single habitat patch of the Colfiorito highlands metapopulation. Our findings indicate that inside a single node, the organization of nesting patches can be conceptualized as a metapopulation itself, where reproductive sites, despite their spatial proximity, can act as either source or sink habitats depending on environmental conditions. We conducted fieldwork in six nesting patches inside a single node, capturing, marking, and recapturing individuals to assess their spatial distribution and movement tendencies at a large landscape scale. We found a high degree of site fidelity among individuals, with many recaptures occurring within the original marking site, but also a sex-based difference in movement patterns; females dispersed farther than males, likely driven by reproductive strategies, while males remained more localized, prioritizing mate-searching. Our findings suggest a complex dynamic in habitat connectivity: pastures and abandoned fields, despite being open, seem to act like sink areas, while breeding sites with shrub and tree cover act as source habitats, offering optimal conditions for reproduction. Individuals, especially females, from these source areas were later compelled to disperse into open habitats, highlighting a nuanced interaction between landscape structure and population dynamics. These results highlight the importance of maintaining habitat corridors to support metapopulation dynamics and prevent genetic isolation; the abandonment of traditional grazing practices is leading to the rapid closure of these source habitats, posing a severe risk of local extinction. Conservation efforts should prioritize the preservation of these source habitats to ensure the long-term viability of E. a. provincialis populations in fragmented landscapes. Full article

Fructose-1,6-bisphosphate aldolase (FBA; EC 4.1.2.13) is a key enzyme in glycolysis and the Calvin cycle, which plays crucial roles in carbon allocation and plant growth. The FBA family genes (FBA s) have been identified in several plants. However, their presence and roles in sweet potato remain unexplored. In this study, a total of 20 FBAs were identified in sweet potato and its wild wild diploidrelatives, including seven in sweet potato (Ipomoea batatas, 2n = 6x = 90), seven in I. trifida (2n = 2x = 30), and six in I. triloba (2n = 2x = 30). Their protein physicochemical properties, chromosomal localization, phylogenetic relationship, gene structure, promoter cis-elements, and expression patterns were systematically analyzed. The conserved genes and protein structures suggest a high degree of functional conservation among FBA genes. IbFBAs may participate in storage root development and starch biosynthesis, especially IbFBA1 and IbFBA6, which warrant further investigation as candidate genes. Additionally, the FBAs could respond to drought and salt stress. They are also implicated in hormone crosstalk, particularly with ABA and GA. This work provides valuable insights into the structure and function of FBAs and identifies candidate genes for improving yield, starch content, and abiotic stress tolerance in sweet potatoes. Full article

Archaeological sites located on the edge of growing cities often struggle to reconcile heritage protection with rapid development. To understand this tension, we examined a 50.83 km² zone around the Western Han Imperial Mausoleums in the Qin-Han New District. Using Landsat images from 1992, 2002, 2012, and 2022, this study applied supervised classification, land-use transfer matrices, and dynamic-degree analysis to trace three decades of land-use change. From 1992 to 2022, built-up land expanded by 29.85 percentage points, largely replacing farmland, which shrank by 35.64 percentage points and became fragmented. Forest cover gained a modest 5.78 percentage points and migrated eastward toward the mausoleums. Overall, urban growth followed a “spread–integrate–connect” pattern along major roads. This study interprets these trends through five interrelated drivers, including policy, planning, economy, population, and heritage protection, and proposes an integrated management model. The model links archaeological pre-assessment with land-use compatibility zoning and active community participation. Together, these measures offer a practical roadmap for balancing conservation and sustainable land management at imperial burial complexes and similar urban fringe heritage sites. Full article

The ecosystem services (ESs) of forests are the benefits that people derive from forest ecosystems. Their precise recognition is important for differentiating and determining the optimal principles of multifunctional forest management. The aim of this study is to identify some important ESs based on a site classification system at the lowest level—i.e., forest stands, at the forest owner level—as a tool for differentiated management. ESs were assessed within the Czech Republic and are expressed in units in accordance with the very sophisticated Forest Site Classification System. (1) Biomass production: The vertical differentiation of ecological conditions given by vegetation tiers, which reflect the influence of altitude, exposure, and climate, provides a basic overview of biomass production; the highest value is in the fourth vegetation tier, i.e., the Fageta abietis community. Forest stands are able to reach a stock of up to 900–1200 m³·ha⁻¹. The lowest production is found in the eighth vegetation tier, i.e., the Piceeta community, with a wood volume of 150–280 m³·ha⁻¹. (2) Soil conservation function: Geological bedrock, soil characteristics, and the geomorphological shape of the terrain determine which habitats serve a soil conservation function according to forest type sets. (3) The hydricity of the site, depending on the soil type, determines the hydric-water protection function of forest stands. Currently, protective forests occupy 53,629 ha in the Czech Republic; however, two subcategories of protective forests—exceptionally unfavorable locations and natural alpine spruce communities below the forest line—potentially account for 87,578 ha and 15,277 ha, respectively. Forests with an increased soil protection function—a subcategory of special-purpose forests—occupy 133,699 ha. The potential area of soil protection forests could be up to 188,997 ha. Water resource protection zones of the first degree—another subcategory of special-purpose forests—occupy 8092 ha, and there is potentially 289,973 ha of forests serving a water protection function (specifically, a water management function) in the Czech Republic. A separate subcategory of water protection with a bank protection function accounts for 80,529 ha. A completely new approach is presented for practical use by forest owners: based on the characteristics of the habitat, they can obtain information about the fulfillment of the habitat’s ecosystem services and, thus, have basic information for the determination of forest categories and the principles of differentiated management. Full article

This paper presents the Green Watershed Index (GWI) methodology, focusing on the 17 sustainability indicators selected in the Razey watershed, NW Iran. Field surveys and data collection have provided the possibility of field inspection and measurement of the present condition of the watershed and the indicators taken. Based on the degree of compliance with the required process, each indicator was scored from 0 to 10 and classified into three categories: unsustainable, semi-sustainable, and sustainable. Using the Entropy method to assign weight to each indicator and formulating a proportional mathematical relationship, the GWI score for each sub-watershed was derived. Spatial changes regarding the selected indicators and, consequently, the GWI were detected in the study area. Development of water infrastructure, particularly in the upstream sub-watersheds, plays a great role in increasing the GWI score. The highest weight is related to environmental productivity (0.26), and the five indicators of water footprint, knowledge management and information quality system, landscape attractiveness, waste recycling, and corruption control have approximately zero weight due to their monotonous spatial distribution throughout sub-watersheds. Only sub-watershed R1 has the highest score (5.13), indicating a semi-sustainable condition. The rest of the sub-watersheds have unsustainable conditions (score below 5). Concerning the GWI, the watershed is facing a critical situation, necessitating the implementation of management and conservation strategies that align with the sustainability level of each sub-watershed. Full article

Alpine scree, a distinctive plateau ecosystem, serves as habitat for numerous rare and endangered species. However, current research does not differentiate it from desert in terms of spatial boundary, hindering biodiversity conservation and ecological monitoring efforts. Using the Tibetan Plateau as a case study, we defined the spatial boundary of alpine scree based on its surface formation process and examined its distribution and long-term evolution. The results show that in 2020, alpine scree on the Tibetan Plateau covered 73,735.34 km², 1.5 times the area of glaciers. Alpine scree is mostly distributed at elevations between 4000 and 6000 m, with a slope of approximately 30–40 degrees. Characterized by low temperature and sparse rainfall, the regions are located in the humid zone. From 1975 to 2020, the area of alpine scree initially increased before declining, with an overall decrease of 560.68 km². Climate warming was the primary driver of these changes, leading to an increase in scree from 1975 to 1995 and a decrease in scree from 1995 to 2020. Additionally, between 1975 and 2020, the Tibetan Plateau’s grasslands shifted upward by 16.47 km². This study enhances our understanding of the spatial distribution and dynamics of this unique ecosystem, alpine scree, offering new insights into climate change impacts on alpine ecosystems. Full article

A reduced representation of a dynamical system helps us to understand what the true degrees of freedom of that system are and thus what the possible instabilities are. Here we extend previous work on barotropic flows to the more general non-barotropic flow case and study the implications for variational analysis and conserved quantities of topological significance such as circulation and helicity. In particular we introduce a four-function Eulerian variational principle of non-barotropic flows, which has not been described before. Also new conserved quantities of non-barotropic flows related to the topological velocity field, topological circulation and topological helicity, including a local version of topological helicity, are introduced. The variational formalism given in terms of a Lagrangian density allows us to introduce canonical momenta and hence a Hamiltonian formalism. Full article

Purpose: Pellucid Marginal Degeneration (PMD) is a rare ectatic corneal disorder characterized by inferior peripheral thinning and significant irregular astigmatism. Despite its clinical similarities to keratoconus, PMD presents unique diagnostic and therapeutic challenges. This review aims to provide a comprehensive update on the pathophysiology, clinical features, diagnostic approaches, and management strategies for PMD, emphasizing the latest advancements in treatment options. Methods: A systematic literature search was performed in MEDLINE (via PubMed), Google Scholar, and Scopus up to February 2025 using the terms: “pellucid marginal degeneration,” “PMD,” “ectatic corneal disorders,” “keratoplasty in PMD,” “corneal cross-linking in PMD,” “ICRS in PMD,” “toric IOL PMD” and their Boolean combinations (AND/OR). The search was restricted to English-language studies involving human subjects, including case reports, case series, retrospective studies, clinical trials, and systematic reviews. A total of 76 studies met the inclusion criteria addressing treatment outcomes in PMD. Results: PMD is characterized by a crescent-shaped band of inferior corneal thinning, leading to high irregular astigmatism and reduced visual acuity. Diagnosis relies on advanced imaging techniques such as Scheimpflug-based corneal tomography, which reveals the characteristic “crab-claw” pattern. Conservative management includes rigid gas-permeable (RGP) lenses and scleral lenses, which provide effective visual rehabilitation in mild to moderate cases. Surgical options, such as CXL, ICRS, and toric IOLs, are reserved for advanced cases, with varying degrees of success. Newer techniques such as CAIRS, employing donor tissue instead of synthetic rings, show promising outcomes in corneal remodeling with potentially improved biocompatibility. Penetrating keratoplasty (PK) and deep anterior lamellar keratoplasty (DALK) remain definitive treatments for severe PMD, though they are associated with significant risks, including graft rejection and postoperative astigmatism. Conclusions: PMD is a complex and progressive corneal disorder that requires a tailored approach to management. Early diagnosis and intervention are critical to optimizing visual outcomes. While conservative measures are effective in mild cases, surgical interventions offer promising results for advanced disease. Further research is needed to refine treatment protocols and improve long-term outcomes for patients with PMD. Full article

Against the backdrop of the green circular economy, the exploration of reliable and sustainable applications of lignocellulosic biomass (LCBM) has emerged as a critical research frontier. The utilization of LCBM as a ruminant roughage source offers a promising strategy to address two pressing issues: the “human-animal competition for food” dilemma and the environmental degradation resulting from improper LCBM disposal. However, the high degree of lignification in LCBM significantly restricts its utilization efficiency in ruminant diets. In recent years, microbial pretreatment has gained considerable attention as a viable approach to reduce lignification prior to LCBM application as ruminant feed. White-rot fungi (WRF) have emerged as particularly noteworthy among various microbial agents due to their environmentally benign characteristics and unique lignin degradation selectivity. WRF demonstrates remarkable efficacy in enzymatically breaking down the rigid lignocellulosic matrix (comprising lignin, cellulose, and hemicellulose) within LCBM cell walls, thereby reducing lignin content—a largely indigestible component for ruminants—while simultaneously enhancing the nutritional profile through increased protein availability and improved digestibility. Solid-state fermentation mediated by WRF enhances LCBM utilization rates and optimizes its nutritional value for ruminant consumption, thereby contributing to the advancement of sustainable livestock production, agroforestry systems, and global environmental conservation efforts. This review systematically examines recent technological advancements in WRF-mediated solid-state fermentation of LCBM, evaluates its outcomes of nutritional enhancement and animal utilization efficiency, and critically assesses current limitations and future prospects of this innovative approach within the framework of circular bioeconomy principles. Full article

The WRKY gene family comprises important transcription factors widely distributed in plants and plays significant roles in the growth and development, diverse (biotic and abiotic) stress responses, and various biological processes. In the current study, 96 identified HvLWRKY genes were classified into three groups and seven subgroups. Among these, 89 genes possessed the conserved domain WRKYGQK. A total of ten motifs were harbored in HvLWRKY genes with two to four introns. Fragmental duplication was suggested to be the prime force that drove the evolution of HvLWRKY genes. A high degree of collinearity was observed between barley and Triticum spelta. Cis-elements of HvLWRKYs were closely associated with abiotic stress, light response, and hormone response; however, there were differences in the numbers among groups. HvLWRKY genes, even the paralogous gene pairs, from different clades were differentially regulated under cold treatments in two landraces. HvLWRKY33, 43, 44, 57, 65, and 77 were homologous with the relative AtWRKY genes in Arabidopsis thaliana. They are suggested to regulate abiotic and pathogen resistance of two barley landraces via SA and JA pathways. Meanwhile, some genes (for example, HvLWRKY1 and HvLWRKY32) were specifically expressed in either cold-tolerant or cold-sensitive landraces. Under cold stress, different cold-responsive patterns occurred in different barley landraces. These findings provide a foundation for further studies on cold resistance in barley landraces and offer new insights for application of WRKY genes in barley breeding. Full article

As an auxin-responsive gene, Gretchen Hagen 3 (GH3) maintains hormonal homeostasis by conjugating excess auxin with amino acids in plant stress-related signaling pathways. GH3 genes have been characterized in many plant species, but the characteristics of pepper (Capsicum annuum L.) GH3 (CaGH3) gene family members in response to multiple stimulants are largely unknown. In this study, we systematically identified the CaGH3 gene family at the genome level and identified eight members on four chromosomes in pepper. CaGH3s were divided into two groups (I and III) and shared conserved motifs, domains, and gene structures. Moreover, CaGH3s had close evolutionary relationships with tomato (Solanum lycopersicum L.), and the promoters of most CaGH3 genes contained hormone and abiotic stress response elements. A protein interaction prediction analysis demonstrated that the CaGH3-3/3-6/3-7/3-8 proteins were possibly core members of the CaGH3 family interaction. In addition, qRT-PCR results showed that CaGH3 genes were differentially expressed in pepper tissues and could be induced by phytohormones (IAA, ABA, and MeJA) and abiotic stresses (salt, low temperature, and drought) with different patterns. In addition, CaGH3-5 and CaGH3-7 were cloned, and the sequences showed a high degree of conservation. Moreover, the results of subcellular localization indicated that they were located in the membrane and chloroplast. Notably, after overexpressing CaGH3-7 in tomato, RNA-seq was performed on wild-type and transgenic lines, and the differentially expressed genes were mainly enriched in response to external stimuli. This study not only lays the foundation for a comprehensive understanding of the function of the CaGH3 gene family during plant growth and stress responses but also provides potential genetic resources for pepper resistance breeding. Full article

Understanding the interplay between plant leaf functional traits and plant and soil factors under different soil thicknesses is significant for quantifying the interaction between plant growth and the environment. However, in the context of ecological restoration of vegetation in mining areas, there has been a lot of research on trees, shrubs, and grasses, but the characteristics and correlations of leaf functional traits of vines have not been fully studied to a large extent. Here, we report the differences in leaf functional traits of six vine plants (Parthenocissus quinquefolia, Pueraria lobata, Hedera nepalensis, Campsis grandiflora, Mucuna sempervirens, and Parthenocissus tricuspidata) with distinct growth forms in different soil cover thicknesses (20 cm, 40 cm, and 60 cm). In addition, soil factor indicators under different soil cover thicknesses were measured to elucidate the linkages between leaf functional traits of vine plants and soil factors. We found that P. lobata showed a resource acquisition strategy, while H. nepalensis demonstrated a resource conservation strategy. C. grandiflora and P. tricuspidata shifted toward more conservative resource allocation strategies as the soil cover thickness increased, whereas M. sempervirens showed the opposite trend. In the plant trait–trait relationships, there were synergistic associations between specific leaf area (SLA) and leaf nitrogen content (LNC); leaf moisture content (LMC) and leaf nitrogen-to-phosphorus ratio (LN/P); and leaf specific dry weight (LSW), leaf succulence degree (LSD), and leaf dry matter content (LDMC). Trade-offs were observed between SLA and LSW, LSD, and LDMC; between leaf phosphorus content (LPC) and LN/P; and between LMC, LSW, and LDMC. In the plant trait–environment relationships, soil nutrients (pH, soil total phosphorus content (STP), and soil ammonium nitrogen content (SAN)) and soil enzyme activities (cellulase (CB), leucine aminopeptidase (LAP), enzyme C/N activity ratio, and enzyme N/P activity ratio) were identified as the primary drivers of variation in leaf functional traits. Interestingly, nitrogen deficiency constrained the growth of vine plants in the mining area. Our study revealed that the responses of leaf functional traits of different vines under different soil thicknesses have significant species specificity, and each vine shows different resource acquisition and conservation strategies. Furthermore, soil cover thickness primarily influences plant functional traits by directly affecting soil enzyme activities and nutrients. However, the pathways through which soil thickness impacts these traits differ among various functional traits. Our findings provide a theoretical basis and practical reference for selecting vine plants and optimizing soil cover techniques for ecological restoration in mining areas. Full article