Search Results (1,716)

Beach sands may harbor human pathogens and antibiotic resistance genes, prompting the proposal of low-dose quicklime (CaO; 1–3% w/w) as a remediation strategy to improve microbiological quality in highly contaminated areas. After application, CaO is converted into calcium carbonate (CaCO₃), yet the ecological effects of this residual compound on benthic fauna remain poorly understood. This study evaluated the short-term impact of CaCO₃-enriched sediment (3% w/w) on the Manila clam, Ruditapes philippinarum, under controlled mesocosm conditions. Adult clams were exposed for one week, and survival, burrowing behavior, feeding- and metabolism-related parameters (clearance, ingestion, absorption efficiency and rate, ammonia excretion), and oxidative stress (malondialdehyde, MDA) were assessed using a hierarchical design, with a tank as the experimental unit. No significant differences were detected between control and CaCO₃-enriched treatments for any measured endpoint. Survival remained high, functional responses showed overlapping ranges, and MDA levels did not differ significantly between groups. Although limited to short-term exposure and a single concentration, these findings suggest that residual CaCO₃ derived from quicklime application did not induce detectable adverse effects in adult R. philippinarum under the tested conditions. Further long-term and multi-species studies are needed to confirm ecological safety. Full article

Stimulating electronic transitions and promoting exciton dissociation are key to enhancing the photocatalytic performance of polymer carbon nitride (PCN). Herein, a controllable synthesis strategy based on supramolecular self-assembly and mild salt melting crystallization has been developed, successfully preparing carbon nitride-based photocatalytic materials with tunable crystal phase composition. The mixed crystal phases effectively induced significant n→π* electronic transition, expanding the material’s light response range to the near-infrared region (700 nm). Meanwhile, the homojunction promoted the efficient separation of photogenerated carriers through the built-in electric field. Under visible-light excitation, this material exhibits excellent selective catalytic performance, over 99% for the oxidation and removal of H₂S into elemental sulfur. This synergistic mechanism of crystal phase engineering in regulating electronic structure and interface charge dynamics provides a new material design strategy for efficient non-metallic photocatalysts. Full article

Introduction: Salmonella may contaminate livestock feed at several stages of production, transport and storage. Formaldehyde is an effective anti-Salmonella feed treatment, but it is now banned for this use in Europe. Organic acid-based additives are an alternative. Gap Statement: The efficacy of organic acid feed additives against natural Salmonella feed contamination is uncertain due to a paucity of reported work investigating low levels of infection that may be relevant for real-world situations. Aim: To compare the anti-Salmonella effects of feed additives based on formaldehyde versus those based on organic acids. Methodology: Experimental contamination of poultry feed with one of three Salmonella serovars at moderate (between 10 and 200 CFU/g) or low (around 1 CFU/g) levels was preceded (‘prevention’ mode) or followed (‘decontamination’ mode) by application of commercial antimicrobial additives. Storage at room temperature for 24 h was followed by pre-enrichment then culture. Results: Organic acid-based products at recommended application rates only eliminated detectable Salmonella from samples with the lowest degree of contamination. The effect was partial, with a proportion of samples still yielding Salmonella in most experiments, and only one such product showed efficacy above 50% of samples for the decontamination mode. The two formaldehyde-based products showed partial efficacy against moderate contamination, and one was entirely effective against low-level contamination even at its lower inclusion rate. Conclusions: Organic acid-based feed additives have a lesser anti-Salmonella effect than formaldehyde-based products at their respective recommended inclusion rates. However, some non-formaldehyde products may be substantially effective against a low, natural degree of contamination. Impact Statement: Chemical suppression of Salmonella in animal feed is an important element of measures to safeguard livestock health and, consequentially, public health too. The European ban on using formaldehyde for this purpose has necessitated the use of alternative products. The present work includes very low levels of Salmonella in feed, possibly mimicking natural contamination, to show that under these circumstances some such alternatives may be as efficacious as formaldehyde products. Full article

Natural gas streams extracted from production wells often contain undesirable components such as water vapor, gas condensate, and solid particulates. These impurities reduce fuel quality and damage downstream equipment through corrosion, fouling, and foaming. This study presents the development and field-scale evaluation of a high-performance gas–liquid separator designed for the deep decontamination of natural gas. The proposed separator incorporates 30 suspended baffles arranged in three rows and an anti-foaming mesh to enhance phase separation and prevent liquid re-entrainment. Field experiments were conducted at the Somontepa gas field in Uzbekistan. Compared to the baseline industrial unit, the upgraded separator reduced gas condensate from 16.58 g/m³ to 0.725 g/m³, water from 4.84 g/m³ to 0.10 g/m³, and solid impurities from 1.20 g/m³ to 0.0058 g/m³. The foam height was lowered from 96.4 mm to 10.2 mm, and the average bubble diameter was reduced by over 60%. The design maintained low pressure drops and demonstrated stable operation under varying flow rates. Fractional analysis confirmed the quality of a recovered condensate suitable for downstream utilization. The proposed configuration offers substantial improvements in gas purification performance and economic efficiency. These results support the application of this separator design for high-contaminant natural gas streams in industrial gas processing facilities. Full article

Burkholderia pseudomallei, the causative agent of melioidosis, presents significant challenges in both treatment and environmental decontamination. Bacteriophages, or phages, are increasingly being explored as potential diagnostic, therapeutic, and biocontrol agents against this bacterial pathogen. Our recent investigation has shown that most B. pseudomallei genomes contained prophage(s) associated with specific tRNA gene loci, prompting us to explore these detectable prophages as sources of temperate phages for further applications. Transcriptomic profiling of B. pseudomallei Bp82, a model strain that possesses three different prophages, revealed high expression levels of the integrase and certain transcriptional regulatory genes within its prophages during normal exponential growth. Using one of its temperate phages, namely φBP82.2, a P2-like phage, as a model, we investigated the lysogenic–lytic control mechanisms. Mutagenesis of the integrase gene, phiBP82.2_gp51, did not improve killing activity compared to the wildtype phage. In contrast, deletion of phiBP82.2_gp38, a putative transcriptional regulatory gene, and two downstream hypothetical protein genes, phiBP82.2_gp36 and phiBP82.2_gp37, resulted in significant lytic improvement. We conclude that these genes play a crucial role in the lysogenic–lytic switch of φBP82.2, suggesting a new avenue for engineering temperate phages for future applications. Full article

Paraburkholderia fungorum (P. fungorum) is an environmental bacterium with biotechnological applications, yet clinical isolations raise concerns about opportunistic infection risk. Genetically related pathogens exhibit substantial antibiotic resistance, motivating the investigation of alternative control strategies. This paper investigates P. fungorum photoinactivation across ultraviolet (222 nm, 254 nm, 313 nm, and 365 nm) and visible (400 nm and 464 nm) wavelengths including ROS (reactive oxygen species) quantification via DCFH-DA fluorescence assay. A two-way ANOVA analysis demonstrated that the wavelength is the dominant determinant of photoinactivation efficacy (F = 100.4, p < 0.001) with ROS generation as a more powerful predictor of inactivation than fluence dose alone (F = 60.6, p < 0.001) at 365 nm, 400 nm, and 464 nm. Ultraviolet irradiation at 254 nm achieved the highest efficiency (5.4 log reduction at 24 mJ/cm²), while 365 nm irradiation demonstrated a high efficacy of 5.2 log reduction at 122 J/cm² with extraordinary ROS production (12,642-fold fluorescence increase). Conversely, inactivation efficiency declined at 400 nm (4.8 log reduction at 378 J/cm² with 122-fold ROS increase) and 464 nm (3.4 log reduction at 3017 J/cm² with lesser ROS detection at 27-fold increase). Wavelength-dependent ROS production correlated directly with bacterial inactivation efficacy, explaining the approximately 500-fold ROS differential between 365 nm and 464 nm. The demonstrated photosensitivity of P. fungorum across multiple wavelengths, with the statistical validation of wavelength-dependent mechanisms, provides a foundation for developing practical, mechanism-based phototherapy protocols tailored to specific clinical and environmental decontamination scenarios. Full article

Ammonia is one of the most important and widely produced basic chemicals worldwide. However, this highly toxic gas is also produced in livestock farming and a variety of industrial processes, posing a potential threat to humans, animals and the environment and also significantly contributing to the formation of persistent particulate matter. The aim of this project was to develop a textile-based adsorber material and to demonstrate a suitable test system for purifying ammonia-contaminated air from production-related sources using the example of pig fattening and PCB production. This aim was achieved through the wash-resistant immobilization of polyacrylic acid on a polyester needle felt at laboratory, pilot plant and industrial scales. In addition, various system concepts have been developed in which air or phosphoric acid can flow through the adsorber textile, whereby in the latter case, the phosphoric acid is both actively involved in ammonia adsorption and also serves to elute the bound ammonia, enabling continuous and low-maintenance operation. Concurrently, the high-quality inorganic fertilizer ammonium phosphate is produced. In summary, an efficient alternative to existing solutions for ammonia minimization has been developed, which is fundamentally characterized by its universal applicability in different load scenarios, including small mobile systems in production facilities with local ammonia pollution, in addition to scenarios for large-scale agricultural operations. Full article

The persistence of per- and polyfluoroalkyl substances (PFASs) poses a significant challenge in solid waste management. This paper systematically reviews the distribution characteristics of PFASs in various solid waste streams, including industrial sludge, food packaging, textiles, and electronic waste. It also evaluates the removal efficiency of four thermal treatment technologies—incineration, pyrolysis, smoldering combustion, and hydrothermal liquefaction (HTL)—for PFASs in solid waste. Although incineration and smoldering combustion can achieve destruction and removal efficiencies exceeding 99.99%, the release of short-chain byproducts remains a critical bottleneck. Pyrolysis effectively decontaminates solid-phase products but carries the risk of phase transfer into pyrolysis oils. The efficiency of HTL is highly dependent on process parameters. PFAS degradation is a radical-mediated process initiated by the dissociation of functional groups. We emphasize that substrate surface properties and the presence of counterions play pivotal roles in modulating these reaction pathways. The introduction of water vapor (as a hydrogen-rich medium), alkaline additives, or specific catalysts is considered a promising strategy to inhibit the recombination of reactive byproducts and enhance mineralization rates. Full article

Firefighters are exposed to complex combustion products and to contaminants carried on personal protective equipment (PPE). Occupational exposure as a firefighter is classified as carcinogenic. This review summarizes the current evidence on exposure environments, routes of uptake, contamination and secondary exposure from PPE, and the effectiveness and limits of decontamination approaches. Across incident types, smoke composition varies with the fuels and combustion conditions, but fine and ultrafine particles and semi-volatile organic chemicals are common. Biomonitoring confirms uptake after incidents. Self-contained breathing apparatus reduces inhalation exposure during active suppression, yet exposures persist through dermal absorption at ensemble interfaces and post-incident tasks. Protective ensembles can retain soot-bound polycyclic aromatic hydrocarbons, additive chemicals, and metals; volatiles and particles resuspension in vehicles and stations can extend exposure. Studies show that on-scene preliminary exposure reduction and laundering can lower contaminant burdens on PPE; however, removal remains incomplete and decreases when cleaning is delayed or when gear is aged. Emerging evidence raises additional concern for per- and polyfluoroalkyl substances from foams and coating materials, with limited data on exposure metrics and removability. The field lacks standardized, realistic contamination platforms and a dose-based definition of clean PPE. Integrated intervention studies linking exposure, secondary exposure pathways, biomarkers, and decontamination methods are needed to set performance-based targets and evaluate emerging hazards. Full article

High-energy ballistic and avulsive injuries to the face represent some of the most complex challenges in modern reconstructive surgery. Since Robertson and Manson’s 1999 management protocol, extensive military experience and technological advancements have transformed the treatment principles while preserving the core tenets of staged care. This updated review synthesizes evidence from 36 studies published since 2000, encompassing over two decades of global experience in both military and civilian trauma. Advances in damage-control resuscitation, wound decontamination, and early skeletal stabilization have improved survival and functional outcomes. Modern imaging—particularly intraoperative CT and navigation—enables the precise verification of the reduction and removal of retained fragments, while virtual surgical planning and patient-specific implants allow the accurate restoration of facial buttresses. Early vascularized tissue transfer has reduced contracture and infection rates. Adjuncts such as hyperbaric oxygen therapy, permissive hypotension, and advanced hemostatic agents further optimize recovery. The updated four-phase protocol—resuscitation, reconstitution, reconstruction, and rehabilitation—emphasizes early definitive repair, multidisciplinary collaboration, and the integration of digital planning. These refinements extend Robertson and Manson’s foundational principles into the era of precision surgery, achieving superior aesthetic and functional outcomes for patients with devastating facial injuries. Full article

Background: Antineoplastic drugs are essential in the treatment of cancer; however, they are classified as hazardous due to their genotoxic, mutagenic, and carcinogenic properties. Healthcare professionals are at risk of exposure primarily through surface contamination. Despite international safety guidelines and technological innovations during the last decades, contamination remains a global occupational health challenge. Objective: This scoping review aims to identify and compare monitoring and detection methods, as well as cleaning and decontamination strategies, in relation to international occupational-safety standards. Methods: Following Arksey and O’Malley’s methodological framework and PRISMA-ScR reporting standards, the peer-reviewed literature and guidelines from 2000 to 2025 were reviewed. Studies were charted across three domains: contamination prevalence, monitoring/detection methods, and cleaning/decontamination effectiveness. Results: Evidence from twenty-two studies conducted in several countries worldwide demonstrated widespread surface contamination across hospital pharmacies, patient-care units, and outpatient facilities. Cyclophosphamide, ifosfamide, and methotrexate were the most frequently detected agents. LC—MS/MS wipe sampling remains the quantitative gold standard, while rapid immunoassay-based tools allow near real-time assessments but with reduced sensitivity. Cleaning protocols varied significantly: oxidizing and surfactant-based agents such as sodium hypochlorite and hydrogen peroxide achieved the highest removal rates (>90%) yet failed to eliminate residues completely. The included studies reported a wide range of monitoring, detection, and cleaning approaches used in healthcare settings. Conclusion: Surface contamination by antineoplastic drugs persists worldwide. Effective management requires harmonized contamination thresholds, validated cleaning strategies, adoption of rapid detection technologies, and continuous occupational surveillance. Full article

Critical metals play a crucial role in advancing sustainable energy technologies, with their demand steadily increasing due to the global push for a circular economy. At present, critical metals are primarily extracted from mineral resources, but critical metal-contaminated soils could often be considered as an alternative source of these elements. There is a growing need for remediation approaches that not only decontaminate soils but also recover valuable metals, thereby aligning with the principles of a circular economy. This review aims to suggest soil remediation strategies able to tackle critical metals contamination, particularly those capable of extracting these elements (dual-purpose technologies). Existing studies indicate that critical metal-contaminated soils are commonly found near mining sites, but their increasing use is spreading these elements into the whole environment. Considering nickel as an example of critical metal, we examine some consolidated technologies which, in addition to remediation, enable the recovery of this critical metal from the soil. Phytomining demonstrated significant potential in extracting nickel from contaminated soils. Electrokinetic treatment and soil washing can be considered as a promising methodology to clean up soils, also facilitating nickel recovery. Full article

Hospital environments host diverse microbial communities that may contribute to nosocomial infections. Moisture-retaining surfaces such as cleaning sponges and faucet edges represent high-contact, under-investigated hygiene hotspots, particularly in wards caring for immunocompromised patients. Environmental samples were collected from cleaning sponges (n = 14) and faucet edges (n = 4) across multiple hospital rooms of a paediatric haematology–oncology unit, with domestic physician sponges as controls (n = 3). DNA was extracted and sequenced targeting the V3–V4 and V7–V9 hypervariable regions of the 16S rRNA gene on the Illumina MiSeq platform. Taxonomic composition and alpha/beta diversity were assessed using QIIME 2 and R. Sponge samples were dominated by Moraxellaceae, particularly Acinetobacter and Enhydrobacter, and showed significantly lower alpha diversity than faucet samples (Shannon index: Kruskal–Wallis H = 8.4, p = 0.01; Faith’s phylogenetic diversity: H = 9.17, p = 0.01). Faucet samples were enriched in human-associated genera including Staphylococcus, Streptococcus, and Chryseobacterium. Statistically significant beta-diversity differences were detected between sponge and faucet communities by PERMANOVA based on Bray–Curtis dissimilarity (p = 0.01), whereas no significant clustering by room or floor location was observed (p = 0.29). Potentially pathogenic taxa including Aeromonas, Pseudomonas, and Enterobacteriaceae were identified across both surface types. Domestic control sponges showed distinct microbiome profiles from hospital samples. Microbial communities differ significantly between hospital sponges and faucets, with surface type rather than location as the primary determinant of community structure. The presence of opportunistic pathogens on both surface types highlights the importance of enhanced hygiene protocols, inclusion of faucet edges and sink drains in routine decontamination schedules, and regular microbiological surveillance in clinical settings caring for immunocompromised patients. Full article

Articular cartilage (AC) damage heals poorly and can progress to osteoarthritis. Implantation of AC fragments (Minced Cartilage Implantation, MCI) is a promising one-step repair technique but is constrained by the limited availability of healthy AC. In this study, we evaluated the feasibility of MCI using nasal septal cartilage (NSC) as an alternative source of hyaline tissue with strong regenerative capacity. NSC obtained from rhinoplasties was decontaminated using a novel protocol, minced with or without Poloxamer 188 (P188), embedded in collagen I gel (0.5 mL per sample), and cultured for 42 days in platelet-rich plasma (PRP)-supplemented medium. The decontamination procedure with a combination of antibiotics was effective and did not impair cell viability. Histology of the resulting constructs confirmed robust cellular outgrowth and matrix deposition. Tissues produced from NSC and fragmented with P188 contained more cartilaginous matrix than those from NSC fragmented without P188 and those from AC fragmented with P188. NSC fragments embedded in a 1 mL hydrogel, sufficient for clinically relevant defect volumes, also demonstrated strong outgrowth and satisfactory matrix formation. Overall, the developed protocol supports the use of NSC as a viable tissue source in gel-based, injectable MCI grafts for focal cartilage repair. Full article

Bismuth oxychloride (BiOCl)– copper oxide (CuO) heterostructures were synthesized via a solvothermal route and assessed as visible-light-driven photocatalysts for methyl orange (MO) degradation. Different CuO loadings deposited on BiOCl microspheres were investigated to identify the optimal composition. Structural and physicochemical characterization revealed that low CuO content (0.6 wt. %) promoted uniform dispersion and enhanced surface area, whereas higher loadings led to nonuniform coverage and reduced photocatalytic efficiency. Operating conditions were optimized using response surface methodology based on a central composite design, considering catalyst dosage (0.1–0.8 g L⁻¹) and pH (4–9). The highest degradation efficiency (~50% after 60 min irradiation) was achieved at pH = 4 and a catalyst dosage of 0.8 g L⁻¹ using the BiOCl surface modified with 0.6% CuO. Kinetic analysis followed a pseudo-first-order model. Mass spectrometry identified transient intermediates associated with demethylation and desulfonation pathways, while radical scavenger experiments confirmed hydroxyl radicals (^•OH) as the dominant oxidizing species, with a secondary contribution from superoxide radicals (^•O₂⁻). These results highlight the critical role of CuO dispersion and interfacial quality in enhancing charge separation and photocatalytic performance, providing practical guidelines for the rational design of BiOX-CuO heterostructures for water remediation applications. Full article