Search Results (78)

Transgenic tobacco plants with additional enzymes producing hydrogen peroxide (H₂O₂)—superoxide dismutase from Arabisopsis thaliana and choline oxidase from Arthrobacter globiformis—have increased resistance to stress factors, which was demonstrated previously, but their reproductive potential has not been studied to date. Superoxide dismutase converts superoxide radical into H₂O₂, and choline oxidase catalyzes the oxidation reaction of choline to form betaine aldehyde, which is subsequently converted into glycine betaine and H₂O₂. We found that the addition of both exogenous genes stimulated growth of the floral organs: petals, styles, and stamens. However, the reproductive potential of the transgenic plants was different. Thus, the introduction of the superoxide dismutase gene FeSOD significantly increased pollen germination in vitro, in vivo, the size of fruits, and the number of seeds. At the same time, the insertion of the CodA gene resulted in the production of abnormal pollen with low germination in vitro. The female reproductive potential system in these plants was not affected. Thus, shifting the ROS balance towards hydrogen peroxide not only increases tobacco stress resistance but also stimulates reproductive success. Glycine betaine production disrupts pollen formation, although such plants show increased resistance to osmotic stress. Full article

Amid growing environmental concerns and the increasing demand for sustainable construction practices, the exploration of alternative materials in building applications has garnered significant attention. This paper provides a comprehensive review of the use of agricultural waste as an aggregate in cementitious composites, with a particular focus on palm kernel shells, coconut shells, hazelnut, peanut and pistachio shells, stone fruit shells and pits, date and grape seeds, rice husks, maize (corn) cobs, and sunflower seed shells. For each type of agro-waste, the paper discusses key physical and mechanical properties, global production volumes, and primary countries of origin. Furthermore, it offers an in-depth analysis of existing research on the incorporation of these materials into cement-based composites, highlighting both the advantages and limitations of their use. Although the integration of agro-waste into construction materials presents certain challenges, the vast quantities of agricultural residues generated globally underscore the urgency and potential of their reuse. In line with circular economy principles, this review advocates for the valorization of agro-waste through innovative and sustainable applications within the construction industry. Full article

Different convergent evolutionary strategies adopted by angiosperm fruits lead to diverse functional seed dispersal units. Dry dehiscent fruits are a common type of fruit, characterized by their lack of fleshy pericarp and the release of seeds at maturity through openings (dehiscence zones, DZs) in their structure. In previous decades, a set of core players in DZ formation have been intensively characterized in Arabidopsis and integrated in a gene regulatory network (GRN) that explains the morphogenesis of these tissues. In this work, we compile all the experimental data available to date to build a discrete Boolean model as a mechanistic approach to validate the network and, if needed, to identify missing components of the GRN and/or propose new hypothetical regulatory interactions, but also to provide a new formal framework to feed further work in Brassicaceae fruit development and the evolution of seed dispersal mechanisms. Hence, by means of exhaustive in-silico validations and experimental evidence, we are able to incorporate both the NO TRANSMITTING TRACT (NTT) transcription factor as a new additional node, and a new set of regulatory hypothetical rules to uncover the dynamics of Arabidopsis DZ specification. Full article

In order to determine the appropriate harvesting period of Carya illinoinensis planted in Nanjing city of China, this study analyzed the phenotypic characteristics and inclusions, including single fruit quality, fruit transverse and vertical diameter, kernel rate, water content, color, respiratory strength, crude fat, soluble sugar, soluble protein, and total phenols, of two cultivars ‘Pawnee’ and ‘Wichita’ during September and October, respectively. Results showed that the respiration intensity and I_AD values of pecan fruits decreased as the harvest date was delayed. ‘Pawnee’ fruits exhibited the highest seed kernel fullness, ∆E value, fruit transverse diameter, shape index, kernel yield, crude fat, and total phenolic content in late September and early October, while the quality of ‘Wichita’ fruits reached its peak in late October. The appropriate harvest period is conducive to the material accumulation of Carya illinoinensis, which is of great practical significance for improving the commodity value of pecans. The optimal harvesting period for ‘Pawnee’ in Nanjing is from the end of September to the beginning of October, and the optimal harvesting period for ‘Wichita’ is from mid- to late-October to the end of October. Full article

Cotton (Gossypium hirsutum) requires a long growing period for fruit and fiber maturation, making it vulnerable to insect pests, thus affecting the seed cotton yield and fiber quality. Cotton-feeding thrips (Thysanoptera: Thripidae) are one of the major insects impacting cotton yield throughout the U.S. cotton belt and worldwide. A two-year field research conducted at Texas A&M AgriLife Research farm in west Texas, USA quantified the interactive effect of three cover crops [wheat (Triticum aestivum), rye (Secale cereale), and no cover] and three irrigation regimes [rainfed, deficit irrigation (30%) and full irrigation] on thrips population dynamics across the phenologically susceptible stages of upland cotton and resulting impact on plant growth and yield parameters. Temporal densities of thrips, feeding injury from thrips, cotton growth and reproductive profiles, yield, and fiber quality varied with cover crops and irrigation levels. Thrips densities were conspicuously low due to harsh weather conditions, but the densities decreased with an increase in plant age. Terminated rye and wheat cover versus conventional-tilled, no-cover treatments showed marginal effects on thrips colonization and population dynamics. Similarly, full irrigation treatment supported higher thrips densities compared to rainfed and deficit irrigation treatments. Immature thrips densities increased through the successive sampling periods, indicating increased thrips reproduction following the initial colonization. Thrips feeding injury was significantly greater in no-cover plots in the early seedling stage, but the effect was insignificant across all cover crop treatments in subsequent sampling dates. The results of this study demonstrated increased seedling vigor, plant height, and flower densities in terminated cover crop plots across all irrigation regimes compared to that in no-cover plots. However, the cover crop x irrigation interaction significantly impacted the cotton lint yield, with increased lint yield on cover crop treatments. This study clearly demonstrates the value of cover crops in semi-arid agricultural production systems that are characterized by low rainfall, reduced irrigation capacity, and wind erosion of topsoil. Full article

The use of instrumental methods of analysis in the assessment of indices that record changes in symmetry in the structure of grains to evaluate the quality of durum and soft wheat grain is currently considered a search tool that will allow us to obtain previously unavailable data by finding correlations associated with differences in the shape and ratio of starch granules in conditionally symmetrical and asymmetrical wheat fruits (kernels) formed in different field conditions and with different genotypes. Indicators that had previously shown their effectiveness were used to analyze the obviously complex unique material obtained as a result of growing under critically unique sowing conditions in 2022, which affected the stability of grain development and filling. For the evaluation, a typical agronomic comparative experiment was chosen, which was used to evaluate the soil tillage practices (fallow, non-moldboard loosening, and plowing) and sowing dates (early and after excessive rainfalls), which made it possible to analyze a wider range of factors influencing the studied indices. The soil tillage methods were found to affect the uniformity of kernel fullness and their symmetry, and the sowing dates did not lead to significant differences. This study presents detailed changes in the shape of the middle cut of a wheat kernel, associated with assessing the efficiency of kernel filling and the symmetrical distribution of storage substances under the influence of external and internal physical factors that affect the formation of the wheat kernel. The data obtained may be of interest to breeders and developers of predictive phenotyping programs for cereal grain and seeds of other crops, as well as plant physiologists. Full article

Date palms are a vital part of oasis ecosystems and are an important source of income in arid and semi-arid areas. Crossbreeding is limited due to the long juvenile stage of date palms and their dioecious nature. The aim of this study was to create triploid date palms to obtain larger and seedless fruits and to increase resilience to abiotic stresses. A tetraploid date palm mutant was crossed with a diploid male palm, yielding hundreds of seeds suspected of containing triploid embryos. Six years after planting, four palms with confirmed triploidy reached maturity. They are phenotypically distinct from diploids, with a thicker rachis, thinner spines, wider and longer midleaf spines, and a longer apical spine. They were classified as sterile bisexual, sterile male and fertile female. One of the latter produced very tasty dates with a very small seed, which is promising for the marketability and profitability of date palm fruits. This first report on triploid date palms provides a way in which to make a significant leap forward in date palm breeding. Given the vigor and fruit quality of female triploid date palms, compared to their diploid counterparts, they will be the target of breeding programs and may spearhead new oases. Full article

Approximately 8 million tons of dates (Phoenix dactylifera) are produced globally each year. The seeds of the fruit, which make up 10–15% of its weight, are typically discarded. Date seed coffee is a sustainable food system innovation rooted in the traditions of high date-producing regions. Dating back to the late 19th century, date seed coffee has evolved from a historical coffee substitute to a modern caffeine-free alternative. Date seed coffee has a long history of consumption in the European Union (EU). This indicates that it may not require novel food authorization. The composition of date seeds is evaluated in this review and a toxicological risk assessment for date seed coffee is conducted. Subchronic studies show that consuming date seed or date seed coffee has no adverse effects. Therefore, currently unavailable chronic toxicity, carcinogenicity, and reproductive toxicity studies may be unnecessary. However, for a comprehensive evaluation, it is recommended to conduct an in vitro mutagenicity test. This review provides information on the safety of date seed coffee and highlights the need for further research. Full article

The determination of grape quality parameters is intricately linked to the mineral composition of the fruit; this relationship is increasingly affected by the impacts of climate change. The conventional chemical methodologies employed for the mineral quantification of grape tissues are expensive and impracticable for widespread commercial applications. This paper utilized Laser-Induced Breakdown Spectroscopy (LIBS) to analyze the mineral constituents within the skin, pulp, and seeds of two distinct Vitis vinifera cultivars: a white cultivar (Loureiro) and a red cultivar (Vinhão). The primary objective was to discriminate the potential variations in the calcium (Ca), magnesium (Mg), and nitrogen (N) concentrations and water content among different grape tissues, explaining their consequential impact on the metabolic constitution of the grapes and, by extension, their influence on various quality parameters. Additionally, the study compared the mineral contents of the white and red grape cultivars across three distinct time points post veraison. Significant differences (p < 0.05) were observed between the Loureiro and Vinhão cultivars in Ca concentrations across all the dates and tissues and for Mg in the skin and pulp, N in the pulp and seeds, and water content in the skin and pulp. In the Vinhão cultivar, Ca differences were found in the pulp across the dates, N in the seeds, and water content in the skin, pulp, and seeds. Comparing the cultivars within tissues, Ca exhibited differences in the pulp, Mg in the skin and pulp, N in the pulp and seeds, and water content in the skin, pulp, and seeds. These findings provide insights into the relationship between the grape mineral and water content, climatic factors, and viticulture practices within a changing climate. Full article

Protein-rich energy bars are known as an excellent nutritional supplement for athletes that help to build and repair connective tissues. The study is, therefore, aimed at developing high-protein bars using lupine seeds, wheat germ, and selected dried fruits including raisins, dates, apricots, and cranberries. Different formulations (F1, F2, F3, and F4) were performed at different ratios of ingredients to produce high-protein bars and compared them with a control bar made of whey-protein concentrate and oat flakes. For this purpose, a proximate analysis, total phenol content, total flavonoid content, DPPH radical scavenging activity, water content, nutritional, and sensory analysis was performed to evaluate the results. The proximate analysis of the produced protein bars showed a significantly higher protein content (22 ± 2) and total phenolic activity (57 ± 33) in formulation group 4 as compared to the other groups. Furthermore, the least water activity content was found in formulation group 1 (1 ± 0.0) when compared with the control group (1 ± 0.0). The results from the sensory evaluation revealed that T3 had the highest average scores in overall consumer acceptability. Our study found that total phenolic, flavonoid, and fiber content were significantly higher in the prepared protein bars indicating prospective health benefits when compared to the control group. Overall, the study demonstrates that high-protein bars using functional ingredients like dried fruit can provide enriched nutritionally valuable food options for consumers. Full article

Date seeds, which are the main by-products of date fruit consumption, were shown to possess promising biological activities and health benefits with minimal human use. The present investigation analyzed and compared the phenolic content of six date seed varieties from four different origins (Khudari, Sakai, and Safawi from Saudi Arabia, Majdool from Jordan, Zahdi from Iraq, and Kabkab from Iran). The aqueous extracts were examined for possible antioxidant, antibacterial, and anti-tumor potential. Date seed oil was extracted, and fatty acid profiles were compared. The results revealed that date seeds are a rich source of polyphenols, which have been linked to biological activities. Furthermore, the phenolic content seemed highly dependent on the variety, where Kabkab had the highest TPC value (271.2 mg GAE/g DM) while Majdool had the lowest value (63.2 mg GAE/g DM). Antioxidant activities of all varieties were highly correlated with the total phenolic content. The antibacterial investigation demonstrated that the Sakai variety possessed the dominant activity, whereas Majdool showed no activity. The results further indicated the sensitivity of both Staphylococcus aureus and Bacillus cereus, with a stronger effect against B. cereus, while no effect was observed against Gram-negative strains (Salmonella Typhi and Escherichia coli). All varieties were able to decrease colon and lung cancer cell viability, especially Khudari and Sakai, with stronger effects against colon cancer cells. Analysis of date seed oil showed high oleic acid content, especially in Sakai. The findings suggest that date seeds are promising candidates for future pharmaceutical applications as nutraceuticals to help combat certain illnesses, as well as functional foods and natural additives that boost the nutritional value of food products, increase their shelf lives, and improve the overall health of consumers. Full article

Background: Bowel habits remain under-studied despite their associations with chronic diseases and their impact on quality of life. We aimed to elucidate the pattern of bowel habits in the UK and investigate gender differences and dietary associations. Methods: A UK population-based survey, “The Big Poo Review,” involving 142,765 participants, was conducted in the ZOE Health Study (LRS/DP-20/21-25809). Respondents completed a 37-item bowel habit questionnaire. Diarrhoea was defined as evacuation >3 times/day or passing Bristol Stool scale (BSS) type 6 or 7 > 25% and constipation was defined as evacuation <3 times/wk or passing BSS type 1 or 2 > 25%. Participants (n = 26,703) who completed a food frequency questionnaire within 5 months of the study were included in the subgroup dietary analysis. Results: Participants were predominantly female (77%) with a mean age of 57.8 years (IQR: 50–67). The most frequently reported bowel pattern was a single daily bowel movement (54%) after breakfast (60%) and BSS type 4 (40%). The mean defecation frequency was 1.7 times/day (SD 0.9), but 0.4% of participants defecated <1 time/wk and 1.4% defecated >4 times/day. Constipation was reported in 21.0% (women 23.3%, men 13.0%; p < 0.001) and diarrhoea in 15.3% (men 17.5%, women 14.7%; p < 0.001). Those with diarrhoea or constipation consumed significantly fewer legumes, nuts, and seeds (12 g and 7 g/day less, respectively), fruits (14 g and 18 g/day less, respectively), and vegetables (14 g and 30 g/day less, respectively) than those without (p < 0.01 for all comparisons). Dairy intake was different between all three groups (constipation 276 g/day; diarrhoea 256 g/day; regular stools 267 g/day; p < 0.001 for all comparisons). Discussion: This survey is the largest study of UK bowel habits to date, highlighting gender and dietary differences in habits. The high prevalence of constipation and diarrhoea underscores the need for focused public health efforts and potential nutrition interventions. Full article

Bamboo propagation and seed collection are severely limited by a protracted and unpredictable flowering cycle. Dendrocalamus hamiltonii Nees et Arn. ex Munro was renowned for its delectable, bitter-free bamboo shoots, making it an exceptional choice for culinary purposes, which has significant economic value in China. To date, no fruit has been collected, and there are currently no comprehensive studies available on the floral morphology and embryology of D. hamiltonii. The morphological and anatomical characteristics of floral organs were described, and the developmental process of male and female gametophytes was elucidated, through anatomical observation. It was found that the floret of D. hamiltonii was composed of one lemma, palea, pistil, and six stamens, but lacked lodicules. The single ovule, possessing a parietal placenta, was anatropous and featured a double integument. The floral organs showed normal in external morphology, whereas male and female gametophyte development was abnormal, such as pollen grain shrinkage, hollow anther chambers, and underdeveloped ovules, which might be the important factors of its low seed setting rate. This study provided important information on the developmental stages of D. hamiltonii flowers and offered a theoretical basis for understanding the low seed setting rate of bamboo. Full article

Wastewater discharge into aquatic systems has become a severe threat to the ecosystem. Herein, Direct Red 28 (DR28) dye removal from an aqueous solution was executed with the application of date fruit seed biochar (DFSB). Fourier transform infrared spectra (FTIR) and scanning electron microscopy (SEM) were utilized for the identification of functional groups and characteristics of the DFSB surface. A series of batch investigations were conducted to analyze pH, contact duration, biochar amount, dye concentration, temperature, and agitation speed on DR28 dye elimination from an aqueous medium by DFSB. The highest dye elimination, 97%, was recorded at a pH of 3 by DFSB at 250 mg/L DR28 dye concentration. The equilibrium data indicated the best fit with the Langmuir isotherm with R² = 0.99, showing 5.83 mg/g monolayer DR28 uptake potential. The best correlation coefficient of the sorption procedure was observed with a pseudo-second-order kinetic study. Investigations on thermodynamic variables disclosed favorable, impetuous exothermic processes. The sorption process was spontaneous as well as exothermic, which was reflected by analyses of thermodynamic parameters. DFSB showed a 33% DR28 dye adsorption ability for up to five successive cycles. DFSB-treated DR28 dye solution increased seedling growth and biochemical components of pigeon pea. The results of the present investigation revealed the significant capacity of DFSB for DR28 dye elimination. Date fruit seed biochar can be applied as an environmentally benign, sustainable adsorbent for DR28 dye removal from industrial effluent, as it is available at zero cost and converts wastewater into reusable biomaterial. Thus, the application of DFSB can assist in wastewater treatment, carbon sequestration, and waste management for a sustainable future. Full article

Plastics have inundated the world, with microplastics (MPs) being small particles, less than 5 mm in size, originating from various sources. They pervade ecosystems such as freshwater and marine environments, soils, and the atmosphere. MPs, due to their small size and strong adsorption capacity, pose a threat to plants by inhibiting seed germination, root elongation, and nutrient absorption. The accumulation of MPs induces oxidative stress, cytotoxicity, and genotoxicity in plants, which also impacts plant development, mineral nutrition, photosynthesis, toxic accumulation, and metabolite production in plant tissues. Furthermore, roots can absorb nanoplastics (NPs), which are then distributed to stems, leaves, and fruits. As MPs and NPs harm organisms and ecosystems, they raise concerns about physical damage and toxic effects on animals, and the potential impact on human health via food webs. Understanding the environmental fate and effects of MPs is essential, along with strategies to reduce their release and mitigate consequences. However, a full understanding of the effects of different plastics, whether traditional or biodegradable, on plant development is yet to be achieved. This review offers an up-to-date overview of the latest known effects of plastics on plants. Full article