Search Results (1,736)

The frescoed Annunziata Oratory chapel in Riofreddo (Italy), a unique testimony to the pontificate of Martin V, sheds light on the trade routes of Ninfa in the first half of the 15th century. Despite having undergone several restorations in the past (the most recent in the 2010s), the Oratory presents serious conservation issues. At first glance, there are no evident signs of biological colonization; rather, the most obvious damage is attributed to detachments and saline efflorescence. Biological colonization at several points was identified using various diagnostic field and laboratory techniques such as ATPase point analysis, field stereoscopy in visible and UV light, culture-based and molecular approaches, Raman spectroscopy, and SEM analysis, biological colonization at several points was identified. The characterization of salt efflorescence was carried out using ion chromatography analysis. The presence of bacteria, fungi and algae, which are also linked to saline efflorescence, was observed. A clear correlation between the biological colonization and salt efflorescence composition was highlighted by our results, as well as the potential sources of microorganisms and salts via the capillary rise of groundwater. This early diagnostic approach regarding the presence of lithobionts and salt efflorescence demonstrates the complex interplay between environmental factors and microbial colonization, which can lead to biodeterioration processes. Full article

Water-related ecosystem services provide a broad range of benefits, including the mitigation of extreme hydrometeorological events, the provision of water for various uses, the support of tourism, and the provision of cultural services. This study assesses the perceptions and accessibility of these services among communities located near the Alafiarou and Okpara dams in Benin and the Bagré dam in Burkina Faso. The methodology involved designing and implementing a questionnaire in KoboCollect, with trained agents deployed to conduct data collection at each of the three sites. Data analysis indicates that respondents identified biodiversity conservation and the provision of drinking water as the most crucial ecosystem services. Over two-thirds of participants reported observing both positive and negative changes in the services provided by rivers and in socio-economic activities since the construction of the dams. While the majority noted improvements in agriculture, irrigation, water quality, fisheries, and flow rates, other changes included biodiversity loss, a decrease in vegetation cover (notably trees and shrubs), an increase in the population of mosquitoes and other insects, and a decline in fishery resources downstream. Despite these challenges, local communities were strongly willing to participate in initiatives aimed at protecting and restoring river ecosystems and their related services. Full article

Cystic fibrosis (CF), the most common hereditary lung disease in Caucasians, is caused by dysfunction of the cystic fibrosis transmembrane conductance regulator (CFTR). We evaluated CFTR function using a newly developed Ussing chamber system, the Multi Trans Epithelial Current Clamp (MTECC), in an in vitro model of human airway epithelia. Air–liquid interface (ALI) cultures were established from nasal brushings of healthy controls (HC) and CF patients with biallelic CFTR variants. ALI layer thickness was similar between groups (HC: 62 ± 13 µm; CF: 55 ± 9 µm). Immunofluorescence showed apical CFTR expression in HC, but reduced or absent signal in CF cultures. MTECC enabled continuous measurement of transepithelial resistance (Rt), potential difference (PD), and conductance (G_t). G_t was significantly reduced in CF cultures compared to HC (0.825 ± 0.024 vs. −0.054 ± 0.016 mS/cm²), indicating impaired cAMP-inducible ion transport by CFTR. Treatment of CF cultures with elexacaftor, tezacaftor, and ivacaftor (Trikafta^®) increased G_t, reflecting partial restoration of CFTR function. These findings demonstrate the utility of MTECC in detecting functional differences in CFTR activity and support its use as a platform for evaluating CFTR-modulating therapies. Our model may contribute to the development of personalized treatment strategies for CF patients. Full article

Objectives: Saccharomyces boulardii CNCM I-745, a probiotic yeast, is effectively used for the treatment of acute diarrhea as well as for the prevention and treatment of traveller‘s diarrhea and diarrhea under tube feeding. The underlying mechanisms are not fully elucidated. Both antitoxic and regulatory effects on the intestinal barrier, mediated either by the yeast or yeast-derived substrates, have been discussed. Methods: To examine the effects of Saccharomyces boulardii released substrates (S.b.S) on gastrointestinal (GI) barrier function, a murine small intestinal organoid cell model under stress was used. Stress was induced by lipopolysaccharide (LPS) exposure or withdrawal of growth factors from cell culture medium (GF_Red). Stressed organoids were treated with S.b.S (200 µg/mL), and markers of GI barrier and inflammatory response were assessed. Results: GF_Red-induced stress was characterized by disturbances in selected tight junction (TJ) (p < 0.05), adherent junction (AJ) (p < 0.001), and mucin (Muc) formation (p < 0.01), measured by gene expressions, whereby additional S.b.S treatment was found to reverse these effects by increasing Muc2 (from 0.22 to 0.97-fold change, p < 0.05), Occludin (Ocln) (from 0.37 to 3.5-fold change, p < 0.0001), and Claudin (Cldn)7 expression (from 0.13 ± 0.066-fold change, p < 0.05) and by decreasing Muc1, Cldn2, Cldn5, and junctional adhesion molecule A (JAM-A) expression (all p < 0.01). Further, S.b.S normalized expression of nucleotide binding oligomerization domain (Nod)2- (from 44.5 to 0.51, p < 0.0001) and matrix metalloproteinase (Mmp)7-dependent activation (from 28.3 to 0.02875 ± 0.0044 ** p < 0.01) of antimicrobial peptide defense and reduced the expression of several inflammatory markers, such as myeloid differentiation primary response 88 (Myd88) (p < 0.01), tumor necrosis factor α (Tnfα) (p < 0.01), interleukin (IL)-6 (p < 0.01), and IL-1β (p < 0.001). Conclusions: Our data provide new insights into the molecular mechanisms by which Saccharomyces boulardii CNCM I-745-derived secretome attenuates inflammatory responses and restores GI barrier function in small intestinal organoids. Full article

Among the approximately 60,000 bone stick fragments unearthed from the Weiyang Palace site of the Han Dynasty, about 57,000 bear inscriptions. Most of these fragments exhibit vertical fractures, leading to a separation between the upper and lower fragments, which poses significant challenges to digital preservation and artifact restoration. Manual matching is inefficient and may cause further damage to the bone sticks. This paper proposes a novel multimodal bone stick matching approach that integrates image, inscription, and archeological information to enhance the accuracy and efficiency of matching fragmented bone stick artifacts. Unlike traditional methods that rely solely on image data, our method leverages large-scale pre-trained models, namely Vision-RWKV for visual feature extraction, RWKV for inscription analysis, and BERT for archeological metadata encoding. A dynamic cross-modal feature fusion mechanism is introduced to effectively combine these features, enabling better interaction and weighting based on the contextual relevance of each modality. This approach significantly improves matching performance, particularly in challenging cases involving fractures, corrosion, and missing sections. The novelty of this method lies in its ability to simultaneously extract and fuse multiple sources of information, addressing the limitations of traditional image-based matching methods. This paper uses Rank-N and Cumulative Match Characteristic (CMC) curves as evaluation metrics. Experimental evaluation shows that the matching accuracy reaches 94.73% at Rank-15, and the method performs significantly better than the comparative methods on the CMC evaluation curve, demonstrating outstanding performance. Overall, this approach significantly enhances the efficiency and accuracy of bone stick artifact matching, providing robust technical support for the research and restoration of bone stick cultural heritage. Full article

Artificial intelligence technologies, particularly machine learning and computer vision, are being increasingly utilized to preserve, restore, and create immersive virtual experiences with cultural artifacts and sites, thus aiding in conserving cultural heritage and making it accessible to a global audience. This paper examines the performance of Generative Adversarial Networks (GAN), especially Style-Based Generator Architecture (StyleGAN), as a deep learning approach for producing realistic images of Egyptian monuments. We used Sigmoid loss for Language–Image Pre-training (SigLIP) as a unique image–text alignment system to guide monument generation through semantic elements. We also studied truncation methods to regulate the generated image noise and identify the most effective parameter settings based on architectural representation versus diverse output creation. An improved discriminator design that combined noise addition with squeeze-and-excitation blocks and a modified MinibatchStdLayer produced 27.5% better Fréchet Inception Distance performance than the original discriminator models. Moreover, differential evolution for latent-space optimization reduced alignment mistakes during specific monument construction tasks by about 15%. We checked a wide range of truncation values from 0.1 to 1.0 and found that somewhere between 0.4 and 0.7 was the best range because it allowed for good accuracy while retaining many different architectural elements. Our findings indicate that specific model optimization strategies produce superior outcomes by creating better-quality and historically correct representations of diverse Egyptian monuments. Thus, the developed technology may be instrumental in generating educational and archaeological visualization assets while adding virtual tourism capabilities. Full article

Human cutaneous leishmaniasis (CL) caused by Leishmania (Viannia) braziliensis is a complex parasitic disease marked by dynamic host–parasite interactions and immunomodulation. Extracellular vesicles (EV) derived from immune cells have emerged as key mediators of intercellular communication and potential biomarkers in infectious diseases. In this study, we combined a modified lymphocyte proliferation assay with nano-flow cytometry to quantify and phenotype EV released by CD4⁺, CD8⁺, and CD14⁺ cells in PBMC cultures from CL patients at different clinical stages: before treatment (PBT), during treatment (PDT), and post-treatment (PET) with antimonial. Healthy individuals (HI) were included as physiological controls. Upon stimulation with L. (V.) braziliensis antigens, we observed a distinct modulation of EV subsets. In the PBT group, CD4⁺ and CD14⁺ EV were significantly reduced, while CD8⁺ EV remained elevated. During PDT and PET, EV concentrations were restored across all subsets. These findings suggest that L. (V.) braziliensis selectively modulates the release of immune cell–derived EV, possibly as an immune evasion mechanism. The restoration of EV release following antimonial therapy highlights their potential as sensitive biomarkers for disease activity and treatment monitoring. This study offers novel insights into the immunoregulatory roles of EV in CL and underscores their relevance in host–parasite interactions. Full article

Museums play an essential role in preserving both cultural and natural heritage, safeguarding samples that offer invaluable insights into our history and scientific understanding. The integration of micro-computed tomography (micro-CT) has significantly advanced the study, restoration, and conservation of these priceless objects. This work explores the application of micro-CT across three critical areas of museum practice: sample virtualization, restoration assessment, and the analysis of fossil specimens. Specifically, micro-CT scanning was applied to fossils stored in the G.A.M.P.S. collection (Scandicci, Italy), enabling the creation of highly detailed non-invasive 3D models for digital archiving and virtual exhibitions. At the Opificio delle Pietre Dure in Florence, micro-CT was employed to evaluate fossil bone restoration treatments, focusing on the internal impact of menthol as a consolidant and its effects on the structural integrity of the material. Furthermore, micro-CT was utilized to investigate a sealed bee preserved in its cocoon within a paleosol in Costa Vicentina (Portugal), providing unprecedented insights into its internal anatomy and state of preservation, all while maintaining the integrity of the specimen. The results of this study underscore the versatility of micro-CT as a powerful non-destructive tool for advancing the fields of conservation, restoration, and scientific analysis of cultural and natural heritage. By integrating high-resolution imaging with both virtual and hands-on conservation strategies, micro-CT empowers museums to enhance research capabilities, improve preservation methodologies, and foster greater public engagement with their collections. Full article

The implementation of the trans-European tourist cycle route network “EuroVelo” is fostering new strategic importance for non-motorized mobility and the associated practice of cycling tourism. Indeed, slow tourism offers a pathway for the development of inland areas. The infrastructure supporting it, such as long-distance cycling and walking paths, can act as a vital connection, stimulating regeneration in peripheral territories by enhancing environmental and landscape assets, as well as preserving heritage, local identity, and culture. The regeneration of peri-urban landscapes through soft mobility is recognized as the cornerstone for accessibility to material and immaterial resources (including ecosystem services) for multiple categories of users, including the most vulnerable, especially following the restoration of green-area systems and non-urbanized areas with degraded ecosystems. Considering the forthcoming implementation of the Magna Grecia cycling route, the southernmost segment of the “EuroVelo” network traversing three regions in southern Italy, this contribution briefly examines the necessity of defining new development policies to effectively integrate sustainable slow tourism with the enhancement of environmental and landscape values in the coastal areas along the route. Specifically, this case study focuses on a coastal stretch characterized by significant morphological and environmental features and notable landscapes interwoven with densely built environments. In this area, environmental and landscape values face considerable threats from scattered, irregular, low-density settlements, abandoned sites, and other inappropriate constructions along the coastline. Full article

There is insufficient evidence regarding the cytotoxicity of restorative 3D-printing resins, used as part of the digital workflow in dentistry. This study presents a novel comparative evaluation of cell viability and adhesion using human Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs), a less commonly used but clinically relevant cell line in dental biomaterials research. The aim of this study was to evaluate the cell viability of WJ-MSCs seeded on 3D-printed resins intended for temporary restorations. Resin discs of three commercial 3D-printing resins (NextDent C&B, Leaf Dental C&B, and UNIZ Temp) and a conventional self-curing acrylic resin (NicTone) were used. WJ-MSCs were cultured on the specimens for 1, 4, and 10 days. Cell viability was assessed using the PrestoBlue assay, Live/Dead immunofluorescence staining, and 7AAD/Annexin V staining. Cell adhesion was evaluated using scanning electron microscopy. Direct exposure to the 3D-printed resins and the self-curing acrylic caused slight reductions in cell viability compared to the control group in both microscopic analyses. 7AAD/Annexin V showed the highest percentage of viable WBCs for the conventional acrylic (34%), followed by UNIZ (35%), NextDent (42%), and Leaf Dental (36%) (ANOVA p < 0.05 Tukey’s post-hoc test p < 0.05). These findings suggest that 3D-printed resins could be considered safe for use in temporary restorations. Full article

Disruption of the intestinal epithelial barrier is a key driver of gut-derived inflammation in various disorders, yet strategies to preserve or restore barrier integrity remain limited. To address this, we evaluated a four-strain Bifidobacterium mixture—selected for complementary anti-inflammatory potency and industrial scalability—in lipopolysaccharide (LPS)-challenged RAW 264.7 macrophages and a Caco-2/THP-1 transwell co-culture model. Pretreatment with the probiotic blend reduced nitric oxide (NO) release in a dose-dependent manner by 25.9–48.3% and significantly down-regulated the pro-inflammatory markers in macrophages. In the co-culture system, the formulation decreased these markers, increased transepithelial electrical resistance (TEER) by up to 31% at 10⁵ colony-forming unit (CFU)/mL after 48 h, and preserved the membrane localization of tight junction (TJ) proteins. Adhesion to Caco-2 cells (≈ 6%) matched that of the benchmark probiotic Lacticaseibacillus rhamnosus GG, suggesting direct epithelial engagement. These in vitro findings demonstrate that this probiotic mixture can attenuate LPS-driven inflammation and reinforce epithelial architecture, providing a mechanistic basis for its further evaluation in animal models and clinical studies of intestinal inflammatory disorders. Full article

Male-factor infertility accounts for approxiamately half of all infertility cases globally, yet therapeutic options remain limited for individuals with no retrievable spermatozoa, such as those with non-obstructive azoospermia (NOA). In recent years, artificial gametogenesis has emerged as a promising avenue for fertility restoration, driven by advances in two complementary strategies: organotypic in vitro spermatogenesis (IVS), which aims to complete spermatogenesis ex vivo using native testicular tissue, and in vitro gametogenesis (IVG), which seeks to generate male gametes de novo from pluripotent or reprogrammed somatic stem cells. To evaluate the current landscape and future potential of these approaches, a narrative, semi-systematic literature search was conducted in PubMed and Scopus for the period January 2010 to February 2025. Additionally, landmark studies published prior to 2010 that contributed foundational knowledge in spermatogenesis and testicular tissue modeling were reviewed to provide historical context. This narrative review synthesizes multidisciplinary evidence from cell biology, tissue engineering, and translational medicine to benchmark IVS and IVG technologies against species-specific developmental milestones, ranging from rodent models to non-human primates and emerging human systems. Key challenges—such as the reconstitution of the blood–testis barrier, stage-specific endocrine signaling, and epigenetic reprogramming—are discussed alongside critical performance metrics of various platforms, including air–liquid interface slice cultures, three-dimensional organoids, microfluidic “testis-on-chip” devices, and stem cell-derived gametogenic protocols. Particular attention is given to clinical applicability in contexts such as NOA, oncofertility preservation in prepubertal patients, genetic syndromes, and reprocutive scenarios involving same-sex or unpartnered individuals. Safety, regulatory, and ethical considerations are critically appraised, and a translational framework is outlined that emphasizes biomimetic scaffold design, multi-omics-guided media optimization, and rigorous genomic and epigenomic quality control. While the generation of functionally mature sperm in vitro remains unachieved, converging progress in animal models and early human systems suggests that clinically revelant IVS and IVG applications are approaching feasibility, offering a paradigm shift in reproductive medicine. Full article

Canvas paintings are prone to biodeterioration due to their complex chemical composition, which can support fungal growth even under controlled conditions. This study evaluated the susceptibility of common synthetic restoration materials—Lascaux glues (303 HV, 498 HV), Acrylharz P550, BEVA 371, Laropal A81, and Regalrez 1094—to degradation by fourteen xerotolerant/xerophilic fungal strains. All tested Aspergillus and Penicillium species extensively colonized, especially artificially aged materials. FTIR-PAS analysis revealed chemical changes in carbonyl and C–H bonds in Laropal A81 and Regalrez 1094 colonized by Aspergillus spp. Scanning electron microscopy (SEM) imaging showed thinning of Lascaux glues and deformation of Regalrez 1094. Transcriptomic profiling of A. puulaauensis grown on Lascaux 498 HV and Regalrez 1094 identified altered expression of genes coding for esterases and oxidases, enzymes involved in synthetic polymer degradation. Esterase activity assays using 4-nitrophenol-based substrates confirmed significant enzymatic activity correlating with the presence of ester bonds. These findings highlight the vulnerability of synthetic restoration materials, specifically Laropal A81, Regalrez 1094, and Lascaux glues, to extremophilic fungi thriving in environments with low water activity. The results emphasize the urgent need for specific knowledge on fungi and their metabolic pathways to use/develop more durable conservation materials and strategies to protect cultural heritage objects from biodeterioration. Full article

Moringa oleifera (MO), a nutritionally and pharmacologically potent species, is emerging as a sustainable candidate for applications across bioenergy, agriculture, textiles, pharmaceuticals, and biomedicine. This review explores recent advances in MO-based biotechnologies, highlighting novel extraction methods, green nanotechnology, and clinical trial findings. Although MO’s resilience offers promise for climate-smart agriculture and public health, challenges remain in standardizing cultivation and verifying therapeutic claims. This work underscores MO’s translational potential and the need for integrative, interdisciplinary research. MO is used in advanced materials, like electrospun fibers and biopolymers, showing filtration, antibacterial, anti-inflammatory, and antioxidant properties—important for the biomedical industry and environmental remediation. In textiles, it serves as an eco-friendly alternative for wastewater treatment and yarn sizing. Biotechnological advancements, such as genome sequencing and in vitro culture, enhance traits and metabolite production. MO supports green biotechnology through sustainable agriculture, nanomaterials, and biocomposites. MO shows potential for disease management, immune support, metabolic health, and dental care, but requires further clinical trials for validation. Its resilience is suitable for land restoration and food security in arid areas. AI and deep learning enhance Moringa breeding, allowing for faster, cost-effective development of improved varieties. MO’s diverse applications establish it as a key element for sustainable development in arid regions. Full article

Psychological stress and dietary behavior are interdependent forces that greatly influence mental and physical health. Thus, both what and how we eat impact our well-being. Maladaptive eating patterns, such as eating in response to emotional cues rather than physiological hunger, have become increasingly common amid modern stressors and an ultra-processed food environment. This narrative review synthesizes interdisciplinary findings from nutritional psychiatry, microbiome science, and behavioral nutrition to explore how stress physiology, gut–brain interactions, and dietary quality shape emotional regulation and eating behavior. It highlights mechanisms (e.g., HPA-axis dysregulation, blunted interoception, and inflammatory and epigenetic pathways) and examines the evidence for mindful and intuitive eating; phytochemical-rich, whole-food dietary patterns; and the emerging role of precision nutrition. Trauma-informed approaches, cultural foodways, structural barriers to healthy eating, and clinical implementation strategies (e.g., interprofessional collaboration) are considered in the context of public health equity to support sustainable mental wellness through dietary interventions. Ultimately, restoring a healthy relationship with food positions nutrition not only as sustenance but as a modifiable regulator of affect, cognition, and stress resilience, central to mental and physical well-being. Full article