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48 pages, 3035 KiB  
Review
A Review of Indian-Based Drones in the Agriculture Sector: Issues, Challenges, and Solutions
by Ranjit Singh and Saurabh Singh
Sensors 2025, 25(15), 4876; https://doi.org/10.3390/s25154876 (registering DOI) - 7 Aug 2025
Abstract
In the current era, Indian agriculture faces a significant demand for increased food production, which has led to the integration of advanced technologies to enhance efficiency and productivity. Drones have emerged as transformative tools for enhancing precision agriculture, reducing costs, and improving sustainability. [...] Read more.
In the current era, Indian agriculture faces a significant demand for increased food production, which has led to the integration of advanced technologies to enhance efficiency and productivity. Drones have emerged as transformative tools for enhancing precision agriculture, reducing costs, and improving sustainability. This study provides a comprehensive review of drone adoption in Indian agriculture by examining its effects on precision farming, crop monitoring, and pesticide application. This research evaluates technological advancements, regulatory frameworks, infrastructure, farmers’ perceptions, and the financial accessibility of drone technology in the Indian agricultural context. Key findings indicate that, while drone adoption enhances efficiency and sustainability, challenges such as high costs, lack of training, and regulatory barriers hinder widespread implementation. This paper also explores the growing market for agricultural drones in India, highlighting key industry players and projected market growth. Furthermore, it addresses regional differences in adoption rates and emphasizes the increasing social acceptance of drones among Indian farmers. To bridge the gap between potential and practice, the study proposes several policy and institutional recommendations, including government-led financial incentives, training programs, and public–private partnerships to facilitate drone integration. Moreover, this review article also highlights technological advancements, such as AI and IoT, in agriculture. Finally, open issues and future research directions for drones are discussed. Full article
(This article belongs to the Section Smart Agriculture)
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23 pages, 7494 KiB  
Article
Temporal and Spatial Evolution of Grey Water Footprint in the Huai River Basin and Its Influencing Factors
by Xi Wang, Yushuo Zhang, Qi Wang, Jing Xu, Fuju Xie and Weiying Xu
Sustainability 2025, 17(15), 7157; https://doi.org/10.3390/su17157157 (registering DOI) - 7 Aug 2025
Abstract
To evaluate water pollution status and sustainable development potential in the Huai River Basin, this study focused on the spatiotemporal evolution and influencing factors of the grey water footprint (GWF) across 35 cities in the basin from 2005 to 2020. This study quantifies [...] Read more.
To evaluate water pollution status and sustainable development potential in the Huai River Basin, this study focused on the spatiotemporal evolution and influencing factors of the grey water footprint (GWF) across 35 cities in the basin from 2005 to 2020. This study quantifies the GWF from agricultural, industrial, and domestic perspectives and analyzes its spatial disparities by incorporating spatial autocorrelation analysis. The Tapio decoupling model was applied to explore the relationship between pollution and economic growth, and geographic detectors along with the STIRPAT model were utilized to identify driving factors. The results revealed no significant global spatial clustering of GWF in the basin, but a pattern of “high in the east and west, low in the north and south” emerged, with high-value areas concentrated in southern Henan and northern Jiangsu. By 2020, 85.7% of cities achieved strong decoupling, indicating improved coordination between the environment and economy. Key driving factors included primary industry output, crop sown area, and grey water footprint intensity, with a notable interaction between agricultural output and grey water footprint intensity. The quantitative analysis based on the STIRPAT model demonstrated that seven factors, including grey water footprint intensity and total crop sown area, exhibited significant contributions to influencing variations. Ranked by importance, these factors were grey water footprint intensity > total crop sown area > urbanization rate > population size > secondary industry output > primary industry output > industrial wastewater discharge, collectively explaining 90.2% of the variability in GWF. The study provides a robust scientific basis for water pollution control and differentiated management in the river basin and holds significant importance for promoting sustainable development of the basin. Full article
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19 pages, 1717 KiB  
Article
A Multifaceted Approach to Optimizing Processed Tomato Production: Investigating the Combined Effects of Biostimulants and Reduced Nitrogen Fertilization
by Michela Farneselli, Lara Reale, Beatrice Falcinelli, Muhammad Zubair Akram, Stefano Cimarelli, Eleonore Cinti, Michela Paglialunga, Flavia Carbone, Euro Pannacci and Francesco Tei
Horticulturae 2025, 11(8), 931; https://doi.org/10.3390/horticulturae11080931 - 7 Aug 2025
Abstract
Excessive nitrogen (N) fertilizer usage in agriculture has prompted the exploration of sustainable strategies to enhance nitrogen use efficiency (NUE) while maintaining crop yield and quality. Processed tomatoes (Solanum lycopersicum L.) were grown for two years (2023 and 2024) following a two-way [...] Read more.
Excessive nitrogen (N) fertilizer usage in agriculture has prompted the exploration of sustainable strategies to enhance nitrogen use efficiency (NUE) while maintaining crop yield and quality. Processed tomatoes (Solanum lycopersicum L.) were grown for two years (2023 and 2024) following a two-way factorial randomized complete block (RCBD) design, considering three biostimulants and three N regimes as two factors, to assess their morphophysiological, biochemical, anatomical and yield performances. Nitrogen application significantly influenced biomass accumulation, the leaf area index (LAI), nitrogen uptake and yield with notable comparable values between reduced and optimal nitrogen dose, indicating improved nitrogen use efficiency. Biostimulants showed limited effects alone but enhanced plant performance under reduced nitrogen conditions, particularly improving chlorophyll content, crop growth, N uptake, yield and anatomical adaptations. Moreover, compared to 2024, biostimulant application enhanced tomato growth more evidently in 2023 due to environmental variations, likely due to the occurrence of stress conditions. Importantly, biostimulants, together with N regimes, i.e., optimal and reduced doses, showed improved anatomical traits, especially regarding leaf thickness and thickness between the two epidermises, indicating adaptive responses that may support sustained productivity under N-limited conditions. Among the biostimulants used, the processed tomatoes responded better to protein hydrolysate and endophytic N-fixing bacteria than to seaweed extract. These findings suggest that although biostimulants alone were not affected, integrating them with reduced N fertilization provides a viable strategy for optimizing tomato production, conserving resources and minimizing the environmental impact without compromising yield or quality. Full article
(This article belongs to the Special Issue Effects of Biostimulants on Horticultural Crop Production)
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14 pages, 1407 KiB  
Article
Black Soldier Fly Frass Fertilizer Outperforms Traditional Fertilizers in Terms of Plant Growth in Restoration in Madagascar
by Cédrique L. Solofondranohatra, Tanjona Ramiadantsoa, Sylvain Hugel and Brian L. Fisher
Sustainability 2025, 17(15), 7152; https://doi.org/10.3390/su17157152 - 7 Aug 2025
Abstract
Black soldier fly frass (BSFF) is a nutrient-rich organic byproduct with growing potential as a sustainable fertilizer. While its effects on crops have been studied, its impact on tree seedling development for reforestation remains poorly understood. This study evaluated the effect of BSFF [...] Read more.
Black soldier fly frass (BSFF) is a nutrient-rich organic byproduct with growing potential as a sustainable fertilizer. While its effects on crops have been studied, its impact on tree seedling development for reforestation remains poorly understood. This study evaluated the effect of BSFF on the growth and survival of two native Malagasy tree species: the fast-growing Dodonaea madagascariensis and the slow-growing Verpis macrophylla. A six-month nursery experiment tested three BSFF application rates (half-, one-, and two-fold nitrogen equivalence), along with cattle manure, synthetic NPK, and a no-fertilizer control. The survival was highest in the half-fold BSFF (95% for D. madagascariensis, 87.5% for V. macrophylla) and lowest in BSFF two-fold (0% and 22.5%, respectively) treatments. NPK also significantly reduced the survival (5% for D. madagascariensis, 17.5% for V. macrophylla). The growth responses were most pronounced in D. madagascariensis, where the BSFF half- and one-fold treatments led to height growth rates that were 2.0–2.7 times higher than that of the control, cattle manure, and NPK treatments, and diameter growth that was 1.8–2.3 times higher. The biomass accumulation was also significantly higher under the BSFF half- and one-fold treatments for D. madagascariensis. In contrast, V. macrophylla showed limited response to the treatments. These findings indicate that calibrated BSFF application can enhance seedling performance in reforestation efforts, particularly for fast-growing species. Notably, the growth rate of D. madagascariensis doubled (in terms of cm/month) under optimal BSFF treatment—a critical advantage, as time is a key constraint in reforestation and faster growth directly supports more efficient forest restoration. This highlights BSFF’s potential as a sustainable and locally available input for forest restoration in Madagascar. Full article
(This article belongs to the Section Sustainability, Biodiversity and Conservation)
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17 pages, 5600 KiB  
Article
From Marshes to Mines: Germination and Establishment of Crinum bulbispermum on Gold Mine Tailings
by Vincent C. Clarke, Sarina Claassens, Dirk P. Cilliers and Stefan J. Siebert
Plants 2025, 14(15), 2443; https://doi.org/10.3390/plants14152443 - 7 Aug 2025
Abstract
The growth potential of Crinum bulbispermum was evaluated on gold mine tailings. The primary objectives were to model the species’ climatic niche in relation to gold mining regions, assess its germination success on tailings, and compare seedling survival and growth on tailings versus [...] Read more.
The growth potential of Crinum bulbispermum was evaluated on gold mine tailings. The primary objectives were to model the species’ climatic niche in relation to gold mining regions, assess its germination success on tailings, and compare seedling survival and growth on tailings versus other soil types. Species distribution modelling identified the South African Grassland Biome on the Highveld (1000+ m above sea level), where the majority of gold mines are located, as highly suitable for the species. Pot trials demonstrated above 85% germination success across all soil treatments, including gold mine tailings, indicating its potential for restoration through direct seeding. An initial seedling establishment rate of 100% further demonstrated the species’ resilience to mine tailings, which are often seasonally dry, nutrient-poor, and may contain potentially toxic metals. However, while C. bulbispermum was able to germinate and establish in mine tailings, long-term growth potential (over 12 months) was constrained by low organic carbon content (0.11%) and high salinity (194.50 mS/m). These findings underscore the critical role of soil chemistry and organic matter in supporting long-term plant establishment and growth on gold tailings. Building on previous research, this study confirms the ability of this thick-rooted geophyte to tolerate chemically extreme soil conditions. Crinum bulbispermum shows promise for phytostabilization and as a potential medicinal plant crop on tailings. However, future research on microbial community interactions and soil amendment strategies is essential to ensure its long-term sustainability. Full article
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22 pages, 2187 KiB  
Article
Long-Term Rotary Tillage and Straw Mulching Enhance Dry Matter Production, Yield, and Water Use Efficiency of Wheat in a Rain-Fed Wheat-Soybean Double Cropping System
by Shiyan Dong, Ming Huang, Junhao Zhang, Qihui Zhou, Chuan Hu, Aohan Liu, Hezheng Wang, Guozhan Fu, Jinzhi Wu and Youjun Li
Plants 2025, 14(15), 2438; https://doi.org/10.3390/plants14152438 - 6 Aug 2025
Abstract
Water deficiency and low water use efficiency severely constrain wheat yield in dryland regions. This study aimed to identify suitable tillage methods and straw management to improve dry matter production, grain yield, and water use efficiency of wheat in the dryland winter wheat–summer [...] Read more.
Water deficiency and low water use efficiency severely constrain wheat yield in dryland regions. This study aimed to identify suitable tillage methods and straw management to improve dry matter production, grain yield, and water use efficiency of wheat in the dryland winter wheat–summer bean (hereafter referred to as wheat-soybean) double-cropping system. A long-term located field experiment (onset in October 2009) with two tillage methods—plowing (PT) and rotary tillage (RT)—and two straw management—no straw mulching (NS) and straw mulching (SM)—was conducted at a typical dryland in China. The wheat yield and yield component, dry matter accumulation and translocation characteristics, and water use efficiency were investigated from 2014 to 2018. Straw management significantly affected wheat yield and yield components, while tillage methods had no significant effect. Furthermore, the interaction of tillage methods and straw management significantly affected yield and yield components except for the spike number. RTSM significantly increased the spike number, grains per spike, 1000-grain weight, harvest index, and grain yield by 12.5%, 8.4%, 6.0%, 3.4%, and 13.4%, respectively, compared to PTNS. Likewise, RTSM significantly increased the aforementioned indicators by 14.8%, 10.1%, 7.5%, 3.6%, and 20.5%, compared to RTNS. Mechanistic analysis revealed that, compared to NS, SM not only significantly enhanced pre-anthesis and post-anthesis dry matter accumulation, and pre-anthesis dry matter tanslocation to grain, but also significantly improved pre-sowing water storage, water consumption during wheat growth, water use efficiency, and water-saving for produced per kg grain yield, with the greatest improvements obtained under RT than PT. Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) analysis confirmed RTSM’s yield superiority was mainly ascribed to straw-induced improvements in dry matter and water productivity. In a word, rotary tillage with straw mulching could be recommended as a suitable practice for high-yield wheat production in a dryland wheat-soybean double-cropping system. Full article
(This article belongs to the Special Issue Emerging Trends in Alternative and Sustainable Crop Production)
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16 pages, 2048 KiB  
Article
Quantitative Determination of Nitrogen Content in Cucumber Leaves Using Raman Spectroscopy and Multidimensional Feature Selection
by Zhaolong Hou, Feng Tan, Manshu Li, Jiaxin Gao, Chunjie Su, Feng Jiao, Yaxuan Wang and Xin Zheng
Agronomy 2025, 15(8), 1884; https://doi.org/10.3390/agronomy15081884 - 4 Aug 2025
Viewed by 201
Abstract
Cucumber, a high-yielding crop commonly grown in facility environments, is particularly susceptible to nitrogen (N) deficiency due to its rapid growth and high nutrient demand. This study used cucumber as its experimental subject and established a spectral dataset of leaves under four nutritional [...] Read more.
Cucumber, a high-yielding crop commonly grown in facility environments, is particularly susceptible to nitrogen (N) deficiency due to its rapid growth and high nutrient demand. This study used cucumber as its experimental subject and established a spectral dataset of leaves under four nutritional conditions, normal supply, nitrogen deficiency, phosphorus deficiency, and potassium deficiency, aiming to develop an efficient and robust method for quantifying N in cucumber leaves using Raman spectroscopy (RS). Spectral data were preprocessed using three baseline correction methods—BaselineWavelet (BW), Iteratively Improve the Moving Average (IIMA), and Iterative Polynomial Fitting (IPF)—and key spectral variables were selected using 4-Dimensional Feature Extraction (4DFE) and Competitive Adaptive Reweighted Sampling (CARS). These selected features were then used to develop a N content prediction model based on Partial Least Squares Regression (PLSR). The results indicated that baseline correction significantly enhanced model performance, with three methods outperforming unprocessed spectra. A further analysis showed that the combination of IPF, 4DFE, and CARS achieved optimal PLSR model performance, achieving determination coefficients (R2) of 0.947 and 0.847 for the calibration and prediction sets, respectively. The corresponding root mean square errors (RMSEC and RMSEP) were 0.250 and 0.368, while the residual predictive deviation (RPDC and RPDP) values reached 4.335 and 2.555. These findings confirm the feasibility of integrating RS with advanced data processing for rapid, non-destructive nitrogen assessment in cucumber leaves, offering a valuable tool for nutrient monitoring in precision agriculture. Full article
(This article belongs to the Section Precision and Digital Agriculture)
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16 pages, 13514 KiB  
Article
Development of a High-Speed Time-Synchronized Crop Phenotyping System Based on Precision Time Protoco
by Runze Song, Haoyu Liu, Yueyang Hu, Man Zhang and Wenyi Sheng
Appl. Sci. 2025, 15(15), 8612; https://doi.org/10.3390/app15158612 - 4 Aug 2025
Viewed by 98
Abstract
Aiming to address the problems of asynchronous acquisition time of multiple sensors in the crop phenotype acquisition system and high cost of the acquisition equipment, this paper developed a low-cost crop phenotype synchronous acquisition system based on the PTP synchronization protocol, realizing the [...] Read more.
Aiming to address the problems of asynchronous acquisition time of multiple sensors in the crop phenotype acquisition system and high cost of the acquisition equipment, this paper developed a low-cost crop phenotype synchronous acquisition system based on the PTP synchronization protocol, realizing the synchronous acquisition of three types of crop data: visible light images, thermal infrared images, and laser point clouds. The paper innovatively proposed the Difference Structural Similarity Index Measure (DSSIM) index, combined with statistical indicators (average point number difference, average coordinate error), distribution characteristic indicators (Charm distance), and Hausdorff distance to characterize the stability of the system. After 72 consecutive hours of synchronization testing on the timing boards, it was verified that the root mean square error of the synchronization time for each timing board reached the ns level. The synchronous trigger acquisition time for crop parameters under time synchronization was controlled at the microsecond level. Using pepper as the crop sample, 133 consecutive acquisitions were conducted. The acquisition success rate for the three phenotypic data types of pepper samples was 100%, with a DSSIM of approximately 0.96. The average point number difference and average coordinate error were both about 3%, while the Charm distance and Hausdorff distance were only 1.14 mm and 5 mm. This system can provide hardware support for multi-parameter acquisition and data registration in the fast mobile crop phenotype platform, laying a reliable data foundation for crop growth monitoring, intelligent yield analysis, and prediction. Full article
(This article belongs to the Special Issue Smart Farming: Internet of Things (IoT)-Based Sustainable Agriculture)
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15 pages, 3854 KiB  
Article
PVC Inhibits Radish (Raphanus sativus L.) Seedling Growth by Interfering with Plant Hormone Signal Transduction and Phenylpropanoid Biosynthesis
by Lisi Jiang, Zirui Liu, Wenyuan Li, Yangwendi Yang, Zirui Yu, Jiajun Fan, Lixin Guo, Chang Guo and Wei Fu
Horticulturae 2025, 11(8), 896; https://doi.org/10.3390/horticulturae11080896 - 3 Aug 2025
Viewed by 233
Abstract
Polyvinyl chloride (PVC) is commonly employed as mulch in agriculture to boost crop yields. However, its toxicity is often overlooked. Due to its chemical stability, resistance to degradation, and the inadequacy of the recycling system, PVC tends to persist in farm environments, where [...] Read more.
Polyvinyl chloride (PVC) is commonly employed as mulch in agriculture to boost crop yields. However, its toxicity is often overlooked. Due to its chemical stability, resistance to degradation, and the inadequacy of the recycling system, PVC tends to persist in farm environments, where it can decompose into microplastics (MPs) or nanoplastics (NPs). The radish (Raphanus sativus L.) was chosen as the model plant for this study to evaluate the underlying toxic mechanisms of PVC NPs on seedling growth through the integration of multi-omics approaches with oxidative stress evaluations. The results indicated that, compared with the control group, the shoot lengths in the 5 mg/L and 150 mg/L treatment groups decreased by 33.7% and 18.0%, respectively, and the root lengths decreased by 28.3% and 11.3%, respectively. However, there was no observable effect on seed germination rates. Except for the peroxidase (POD) activity in the 150 mg/L group, all antioxidant enzyme activities and malondialdehyde (MDA) levels were higher in the treated root tips than in the control group. Both transcriptome and metabolomic analysis profiles showed 2075 and 4635 differentially expressed genes (DEGs) in the high- and low-concentration groups, respectively, and 1961 metabolites under each treatment. PVC NPs predominantly influenced seedling growth by interfering with plant hormone signaling pathways and phenylpropanoid production. Notably, the reported toxicity was more evident at lower concentrations. This can be accounted for by the plant’s “growth-defense trade-off” strategy and the manner in which nanoparticles aggregate. By clarifying how PVC NPs coordinately regulate plant stress responses via hormone signaling and phenylpropanoid biosynthesis pathways, this research offers a scientific basis for assessing environmental concerns related to nanoplastics in agricultural systems. Full article
(This article belongs to the Special Issue Stress Physiology and Molecular Biology of Vegetable Crops)
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16 pages, 3523 KiB  
Article
Vegetation Composition and Environmental Relationships of Two Amaranthus Species Communities in Variant Agroecosystems at Fayoum Depression, Egypt
by Mai Sayed Fouad, Manar A. Megahed, Nabil A. Abo El-Kassem, Hoda F. Zahran and Abdel-Nasser A. A. Abdel-Hafeez
Diversity 2025, 17(8), 551; https://doi.org/10.3390/d17080551 - 3 Aug 2025
Viewed by 205
Abstract
Amaranthus is appointed as a common weed associated with crops. The research was designed to survey the Amaranth existence pattern throughout the Fayoum Depression, Egypt, accompanied with a community vegetation analysis. The study was extended to collect and analyze associated soil samples. The [...] Read more.
Amaranthus is appointed as a common weed associated with crops. The research was designed to survey the Amaranth existence pattern throughout the Fayoum Depression, Egypt, accompanied with a community vegetation analysis. The study was extended to collect and analyze associated soil samples. The obtained results figured out the prevalence of dicot families, herb growth forms, therophyte followed by phanerophyte life forms, the Pantropical monoregional chorotype, and the Mediterranean and Sudano-Zambezian followed by the Irano-Turanian pluri-regional chorotype. Multilevel pattern analysis stated that Gossypium barbadense, Corchorus olitorius, Sorghum bicolor, Sesamum indicum, and Zea mays are indicator species most related to Amaranth occurrence and prediction. NMDS analysis denoting that the Ibshaway, Youssef Al Seddik, Itsa, and Fayoum districts are the most representative districts for Amaranth existence on the basis of edaphic resources. Itsa and Youssef Al Seddik, in addition to Itsa and Fayoum, resemble each other in species composition. High pH and CaCO3 percentages were discriminatory in Ibshaway, Itsa, and Youssef Al Seddik. Ni was the cornerstone for districts partitioning in pruned trees. Finally, Amaranth was flourishing in both comfortable and harsh habitats with cultivated crops and orchards, as well as on the outskirts. The findings are considered to be valorized by decision makers in arable land management. Full article
(This article belongs to the Section Plant Diversity)
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20 pages, 3604 KiB  
Article
Analysis of the Differences in Rhizosphere Microbial Communities and Pathogen Adaptability in Chili Root Rot Disease Between Continuous Cropping and Rotation Cropping Systems
by Qiuyue Zhao, Xiaolei Cao, Lu Zhang, Xin Hu, Xiaojian Zeng, Yingming Wei, Dongbin Zhang, Xin Xiao, Hui Xi and Sifeng Zhao
Microorganisms 2025, 13(8), 1806; https://doi.org/10.3390/microorganisms13081806 - 1 Aug 2025
Viewed by 229
Abstract
In chili cultivation, obstacles to continuous cropping significantly compromise crop yield and soil health, whereas crop rotation can enhance the microbial environment of the soil and reduce disease incidence. However, its effects on the diversity of rhizosphere soil microbial communities are not clear. [...] Read more.
In chili cultivation, obstacles to continuous cropping significantly compromise crop yield and soil health, whereas crop rotation can enhance the microbial environment of the soil and reduce disease incidence. However, its effects on the diversity of rhizosphere soil microbial communities are not clear. In this study, we analyzed the composition and characteristics of rhizosphere soil microbial communities under chili continuous cropping (CC) and chili–cotton crop rotation (CR) using high-throughput sequencing technology. CR treatment reduced the alpha diversity indices (including Chao1, Observed_species, and Shannon index) of bacterial communities and had less of an effect on fungal community diversity. Principal component analysis (PCA) revealed distinct compositional differences in bacterial and fungal communities between the treatments. Compared with CC, CR treatment has altered the structure of the soil microbial community. In terms of bacterial communities, the relative abundance of Firmicutes increased from 12.89% to 17.97%, while the Proteobacteria increased by 6.8%. At the genus level, CR treatment significantly enriched beneficial genera such as RB41 (8.19%), Lactobacillus (4.56%), and Bacillus (1.50%) (p < 0.05). In contrast, the relative abundances of Alternaria and Fusarium in the fungal community decreased by 6.62% and 5.34%, respectively (p < 0.05). Venn diagrams and linear discriminant effect size analysis (LEfSe) further indicated that CR facilitated the enrichment of beneficial bacteria, such as Bacillus, whereas CC favored enrichment of pathogens, such as Firmicutes. Fusarium solani MG6 and F. oxysporum LG2 are the primary chili root-rot pathogens. Optimal growth occurs at 25 °C, pH 6: after 5 days, MG6 colonies reach 6.42 ± 0.04 cm, and LG2 5.33 ± 0.02 cm, peaking in sporulation (p < 0.05). In addition, there are significant differences in the utilization spectra of carbon and nitrogen sources between the two strains of fungi, suggesting their different ecological adaptability. Integrated analyses revealed that CR enhanced soil health and reduced the root rot incidence by optimizing the structure of soil microbial communities, increasing the proportion of beneficial bacteria, and suppressing pathogens, providing a scientific basis for microbial-based soil management strategies in chili cultivation. Full article
(This article belongs to the Section Microbiomes)
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19 pages, 5891 KiB  
Article
Potential of Multi-Source Multispectral vs. Hyperspectral Remote Sensing for Winter Wheat Nitrogen Monitoring
by Xiaokai Chen, Yuxin Miao, Krzysztof Kusnierek, Fenling Li, Chao Wang, Botai Shi, Fei Wu, Qingrui Chang and Kang Yu
Remote Sens. 2025, 17(15), 2666; https://doi.org/10.3390/rs17152666 - 1 Aug 2025
Viewed by 167
Abstract
Timely and accurate monitoring of crop nitrogen (N) status is essential for precision agriculture. UAV-based hyperspectral remote sensing offers high-resolution data for estimating plant nitrogen concentration (PNC), but its cost and complexity limit large-scale application. This study compares the performance of UAV hyperspectral [...] Read more.
Timely and accurate monitoring of crop nitrogen (N) status is essential for precision agriculture. UAV-based hyperspectral remote sensing offers high-resolution data for estimating plant nitrogen concentration (PNC), but its cost and complexity limit large-scale application. This study compares the performance of UAV hyperspectral data (S185 sensor) with simulated multispectral data from DJI Phantom 4 Multispectral (P4M), PlanetScope (PS), and Sentinel-2A (S2) in estimating winter wheat PNC. Spectral data were collected across six growth stages over two seasons and resampled to match the spectral characteristics of the three multispectral sensors. Three variable selection strategies (one-dimensional (1D) spectral reflectance, optimized two-dimensional (2D), and three-dimensional (3D) spectral indices) were combined with Random Forest Regression (RFR), Support Vector Machine Regression (SVMR), and Partial Least Squares Regression (PLSR) to build PNC prediction models. Results showed that, while hyperspectral data yielded slightly higher accuracy, optimized multispectral indices, particularly from PS and S2, achieved comparable performance. Among models, SVM and RFR showed consistent effectiveness across strategies. These findings highlight the potential of low-cost multispectral platforms for practical crop N monitoring. Future work should validate these models using real satellite imagery and explore multi-source data fusion with advanced learning algorithms. Full article
(This article belongs to the Special Issue Perspectives of Remote Sensing for Precision Agriculture)
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28 pages, 4026 KiB  
Article
Multi-Trait Phenotypic Analysis and Biomass Estimation of Lettuce Cultivars Based on SFM-MVS
by Tiezhu Li, Yixue Zhang, Lian Hu, Yiqiu Zhao, Zongyao Cai, Tingting Yu and Xiaodong Zhang
Agriculture 2025, 15(15), 1662; https://doi.org/10.3390/agriculture15151662 - 1 Aug 2025
Viewed by 244
Abstract
To address the problems of traditional methods that rely on destructive sampling, the poor adaptability of fixed equipment, and the susceptibility of single-view angle measurements to occlusions, a non-destructive and portable device for three-dimensional phenotyping and biomass detection in lettuce was developed. Based [...] Read more.
To address the problems of traditional methods that rely on destructive sampling, the poor adaptability of fixed equipment, and the susceptibility of single-view angle measurements to occlusions, a non-destructive and portable device for three-dimensional phenotyping and biomass detection in lettuce was developed. Based on the Structure-from-Motion Multi-View Stereo (SFM-MVS) algorithms, a high-precision three-dimensional point cloud model was reconstructed from multi-view RGB image sequences, and 12 phenotypic parameters, such as plant height, crown width, were accurately extracted. Through regression analyses of plant height, crown width, and crown height, and the R2 values were 0.98, 0.99, and 0.99, respectively, the RMSE values were 2.26 mm, 1.74 mm, and 1.69 mm, respectively. On this basis, four biomass prediction models were developed using Adaptive Boosting (AdaBoost), Support Vector Regression (SVR), Gradient Boosting Decision Tree (GBDT), and Random Forest Regression (RFR). The results indicated that the RFR model based on the projected convex hull area, point cloud convex hull surface area, and projected convex hull perimeter performed the best, with an R2 of 0.90, an RMSE of 2.63 g, and an RMSEn of 9.53%, indicating that the RFR was able to accurately simulate lettuce biomass. This research achieves three-dimensional reconstruction and accurate biomass prediction of facility lettuce, and provides a portable and lightweight solution for facility crop growth detection. Full article
(This article belongs to the Section Crop Production)
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20 pages, 2990 KiB  
Article
Examination of Interrupted Lighting Schedule in Indoor Vertical Farms
by Dafni D. Avgoustaki, Vasilis Vevelakis, Katerina Akrivopoulou, Stavros Kalogeropoulos and Thomas Bartzanas
AgriEngineering 2025, 7(8), 242; https://doi.org/10.3390/agriengineering7080242 - 1 Aug 2025
Viewed by 198
Abstract
Indoor horticulture requires a substantial quantity of electricity to meet crops extended photoperiodic requirements for optimal photosynthetic rate. Simultaneously, global electricity costs have grown dramatically in recent years, endangering the sustainability and profitability of indoor vertical farms and/or modern greenhouses that use artificial [...] Read more.
Indoor horticulture requires a substantial quantity of electricity to meet crops extended photoperiodic requirements for optimal photosynthetic rate. Simultaneously, global electricity costs have grown dramatically in recent years, endangering the sustainability and profitability of indoor vertical farms and/or modern greenhouses that use artificial lighting systems to accelerate crop development and growth. This study investigates the growth rate and physiological development of cherry tomato plants cultivated in a pilot indoor vertical farm at the Agricultural University of Athens’ Laboratory of Farm Structures (AUA) under continuous and disruptive lighting. The leaf physiological traits from multiple photoperiodic stress treatments were analyzed and utilized to estimate the plant’s tolerance rate under varied illumination conditions. Four different photoperiodic treatments were examined and compared, firstly plants grew under 14 h of continuous light (C-14L10D/control), secondly plants grew under a normalized photoperiod of 14 h with intermittent light intervals of 10 min of light followed by 50 min of dark (NI-14L10D/stress), the third treatment where plants grew under 14 h of a load-shifted energy demand response intermittent lighting schedule (LSI-14L10D/stress) and finally plants grew under 13 h photoperiod following of a load-shifted energy demand response intermittent lighting schedule (LSI-13L11D/stress). Plants were subjected also under two different light spectra for all the treatments, specifically WHITE and Blue/Red/Far-red light composition. The aim was to develop flexible, energy-efficient lighting protocols that maintain crop productivity while reducing electricity consumption in indoor settings. Results indicated that short periods of disruptive light did not negatively impact physiological responses, and plants exhibited tolerance to abiotic stress induced by intermittent lighting. Post-harvest data indicated that intermittent lighting regimes maintained or enhanced growth compared to continuous lighting, with spectral composition further influencing productivity. Plants under LSI-14L10D and B/R/FR spectra produced up to 93 g fresh fruit per plant and 30.4 g dry mass, while consuming up to 16 kWh less energy than continuous lighting—highlighting the potential of flexible lighting strategies for improved energy-use efficiency. Full article
(This article belongs to the Topic Digital Agriculture, Smart Farming and Crop Monitoring)
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16 pages, 2729 KiB  
Article
Effect of Enterobacter bugandensis R-18 on Maize Growth Promotion Under Salt Stress
by Xingguo Tian, Qianru Liu, Jingjing Song, Xiu Zhang, Guoping Yang, Min Li, Huan Qu, Ahejiang Tastanbek and Yarong Tan
Microorganisms 2025, 13(8), 1796; https://doi.org/10.3390/microorganisms13081796 - 31 Jul 2025
Viewed by 270
Abstract
Soil salinization poses a significant constraint to agricultural productivity. However, certain plant growth-promoting bacteria (PGPB) can mitigate salinity stress and enhance crop performance. In this study, a bacterial isolate, R-18, isolated from saline-alkali soil in Ningxia, China, was identified as Enterobacter bugandensis based [...] Read more.
Soil salinization poses a significant constraint to agricultural productivity. However, certain plant growth-promoting bacteria (PGPB) can mitigate salinity stress and enhance crop performance. In this study, a bacterial isolate, R-18, isolated from saline-alkali soil in Ningxia, China, was identified as Enterobacter bugandensis based on 16S rRNA gene sequencing. The isolate was characterized for its morphological, biochemical, and plant growth-promoting traits and was evaluated for its potential to alleviate NaCl-induced stress in maize (Zea mays L.) under hydroponic conditions. Isolate R-18 exhibited halotolerance, surviving at NaCl concentrations ranging from 2.0% to 10.0%, and alkaliphilic adaptation, growing at pH 8.0–11.0. Biochemical assays confirmed it as a Gram-negative bacterium, displaying positive reactions in the Voges–Proskauer (V–P) tests, catalase activity, citrate utilization, fluorescent pigment production, starch hydrolysis, gelatin liquefaction, and ammonia production, while testing negative for the methyl red and cellulose hydrolysis. Notably, isolate R-18 demonstrated multiple plant growth-promoting attributes, including nitrogen fixation, phosphate and potassium solubilization, ACC deaminase activity, and indole-3-acetic acid (IAA) biosynthesis. Under 100 mM NaCl stress, inoculation with isolate R-18 significantly enhanced maize growth, increasing plant height, stem dry weight, root fresh weight, and root dry weight by 20.64%, 47.06%, 34.52%, and 31.25%, respectively. Furthermore, isolate R-18 improved ion homeostasis by elevating the K+/Na+ ratio in maize tissues. Physiological analyses revealed increased chlorophyll and proline content, alongside reduced malondialdehyde (MDA) levels, indicating mitigated oxidative damage. Antioxidant enzyme activity was modulated, with decreased superoxide dismutase (SOD) and peroxidase (POD) activities but increased catalase (CAT) activity. These findings demonstrated that Enterobacter bugandensis R-18 effectively alleviated NaCl-induced growth inhibition in maize by enhancing osmotic adjustment, reducing oxidative stress, and improving ion balance. Full article
(This article belongs to the Section Plant Microbe Interactions)
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