Search Results (12,200)

This study examines how climate change interacts with social, ecological, and policy factors to shape food security among Indigenous tribal communities in Jharkhand, focusing on Saraikela Kharsawan district. It combines a scoping review, policy analysis, and a climate–agriculture case study of Saraikela Kharsawan to identify vulnerabilities and pathways for more resilient Indigenous food systems. The research is qualitative, using a scoping review of 28 studies on Indigenous food security and climate impacts in Jharkhand, thematic analysis of nine national and state policies, and a district-level case study using land use, climate trends/projections, and crop statistics for Saraikela Kharsawan. Additionally, findings from participant observation were integrated into how tribal communities in Saraikela Kharsawan experience and respond to climate variability and its implications for local food systems and nutrition. The study identifies a nutrition paradox, where Indigenous communities experience micronutrient deficiencies and anaemia despite rich biodiversity and Indigenous knowledge. This is accompanied by a decrease in the consumption of nutrient-dense Indigenous foods and a predominance of rainfed monoculture rice cultivation. Marked by rising temperatures and erratic rainfall, climate variability is destabilising agroforestry systems, narrowing dietary options and reducing adaptive capacity. Additionally, policy and institutional gaps reveal fragmented support—strong rights laws and calorie-focused welfare schemes but weak integration of Indigenous foods, agroforestry, and traditional ecological knowledge into nutrition and climate programmes. The paper argues that climate change acts as a threat multiplier on already fragile Indigenous food systems and calls for nutrition-sensitive safety nets, community-based agroforestry, gender-inclusive Indigenous knowledge governance, and cross-sectoral policy alignment to support resilient, culturally appropriate food systems in Jharkhand. Full article

Antimicrobial resistance (AMR) is a global health crisis addressed through a One Health framework. However, recent European Union (EU) surveillance data reveals a marked divergence in progress between the human and animal sectors. This study analyzes the most recent monitoring reports (European Surveillance of Antimicrobial Consumption Network and European Sales and Use of Antimicrobials for Veterinary Medicine, 2024) to compare the effectiveness of mitigation strategies across sectors. The findings expose a clear paradox: while the veterinary sector has achieved a structural 24.3% reduction in antimicrobial sales in the EU since 2018, human medicine has recorded a 2% increase in overall consumption, diverging from established reduction targets. From a qualitative perspective, veterinary medicine has nearly eliminated the use of critically important antimicrobials in the AntiMicrobial Expert Group (AMEG) (category B), including polymyxins and third-generation cephalosporins, which now account for only 0.24% of total sales. In contrast, human medicine continues to struggle to contain antimicrobial resistance in key sentinel pathogens, notably Klebsiella pneumoniae and Escherichia coli. Furthermore, companion animals, representing 97.9% of non-food-producing animal biomass, emerge as a reservoir of antimicrobial-resistant bacteria due to the intensive use of broad-spectrum oral formulations. The results indicate that the veterinary regulatory model, centered on binding volume reduction and preventive strategies, has been more effective in reducing overall antimicrobial consumption compared to the voluntary, guideline-based stewardship approaches currently used in human medicine. Achieving meaningful control of antimicrobial resistance will require human medicine to adopt the same level of structural rigor already implemented in animal production systems. Full article

The safety of non-food products is embedded in the United Nations 2030 Agenda for Sustainable Development and the European Union (EU) framework, supporting health protection, responsible production and consumption, and market surveillance. The EU Rapid Alert System for dangerous non-food products, known as Safety Gate (formerly RAPEX), was established in 2005 to facilitate the exchange of information on products posing risks within the internal market. The aim of this study was to present the interdependencies reported in the Safety Gate system/RAPEX in 2005–2025, considering: product category, type of risk, country of origin, notifying country and year, as well as measures taken. The VOSviewer 1.6.20 and Statistica 13.3 were used. The results highlighted the following problems: toys from China with chemical, choking and injury risks; electrical appliances also from China with electric shock hazards; motor vehicles from Germany with injury risks; cosmetics from Italy with chemical and microbiological risks; and clothing from Turkey with suffocation risks. Reporting is expected to continue under existing regulatory frameworks, although changing the name of the system from RAPEX to “Safety Gate” may reduce its recognition. The findings highlight the need for targeted enforcement, improved risk profiling by product category and origin, and ongoing monitoring of emerging safety risks. Full article

This paper explores the culinary and cultural significance of cooked leafy vegetables among Afro-descendant communities in French Guiana and Suriname, including French Guianese and Surinamese Creoles, Maroons, and Haitian migrants. While leafy greens play a major dietary role across sub-Saharan Africa, their consumption in the Americas remains understudied. This ethnobotanical study of edible leafy plants is based on surveys of local markets, gardens and residents. Drawing on 26 informal interviews conducted in four local languages (French, French Guianese Creole, Haitian Creole, and Nengee Tongo), we describe 36 species of edible leaves from 20 plant families consumed in the region. Our findings show that although the practice of eating leafy greens is widely shared, the species selected, their names, and their perceived properties vary noticeably across cultural groups. Some plants are eaten exclusively by Maroons (e.g., Cestrum latifolium, Capsicum spp.), others by Haitians (e.g., Corchorus olitorius, Rivina humilis), and some have fallen into disuse among younger generations. These differences are shaped by ecological availability, cultural memory, food-medicine beliefs, and interethnic influences. We suggest that the term callaloo (referring to both dishes and leafy vegetables), which circulates in multiple linguistic and culinary forms throughout the African diaspora, can serve as a metaphor for the interculturalization of foodways. More than ingredients, these leafy vegetables act as dynamic cultural markers—symbols of resilience, transmission, and transformation. In a context of rapid globalization, where unseen foods risk sinking further into obscurity, these plant-based traditions highlight both the adaptability and fragility of Afro-descendant culinary heritage in the Guiana Shield. Full article

Background/Objectives: Fortified eggs have been proposed as food-based vehicles for delivering micronutrients and other bioactive compounds. However, human intervention evidence remains fragmented across nutrient targets, populations, intervention designs, and outcome domains. This review mapped human intervention studies and summarized the reported nutritional and health-related outcomes associated with fortified egg consumption. Methods: PubMed, Embase, Web of Science, the Cochrane Library, CNKI, Wanfang Data, and SinoMed were searched for studies published in English or Chinese from 1 January 2005 to 1 June 2025. Eligible studies were human intervention studies evaluating fortified, enriched, or bio-enhanced eggs and reporting at least one nutritional or health-related outcome. Owing to substantial heterogeneity in nutrient type, intervention design, comparator, duration, and outcome assessment, findings were synthesized narratively. Results: Thirty-seven human intervention studies met the eligibility criteria. Most evaluated eggs enriched with n-3 polyunsaturated fatty acids, followed by carotenoid-enriched eggs, whereas relatively few examined iodine or other fortified nutrients. Across studies, fortified egg consumption was generally associated with improvements in intake estimates and biomarkers of targeted nutrient status, including circulating fatty acids, serum carotenoids, and, in a limited number of studies, urinary iodine. Evidence for downstream health-related outcomes was more limited, inconsistent, and difficult to compare across nutrient categories and study populations. Conclusions: Fortified eggs may serve as useful food-based vehicles for improving the delivery of selected nutrients and short-term nutrient-status biomarkers. However, the evidence base remains heterogeneous and nutrient-specific, and it is still uncertain whether these changes translate into consistent, clinically meaningful health benefits. Larger, longer-term, and more rigorously reported trials, particularly in populations at nutritional risk, are needed. Full article

Background: Eating behaviors and psychological processes related to food consumption are increasingly recognized as key factors of successful adherence to a healthy dietary pattern. In this context, mindful eating and adherence to the Mediterranean Diet have emerged as models promoting beneficial health outcomes, whereas orthorexia nervosa, characterized by an obsessive focus on eating healthy foods, often leads to dietary inadequacies. Here, we investigated the association among mindful eating, adherence to the Mediterranean Diet, orthorexia nervosa and BMI in a cohort of university students. Methods: A sample of 518 Italian university students completed an online survey assessing general and sociodemographic characteristics, the adherence to the Mediterranean Diet pattern by MEDAS and MEDLIFE, the orthorexia tendency by ORTO-15 test and the degree of mindful eating by the Mind-Eat Scale. Students’ t-test, chi-square test, Pearson’s correlation, and K-means cluster analysis were used for analyses. Results: The participants had a mean BMI of 23.08 ± 3.51, with 69% normal-weight and 25% overweight/obese individuals, showing statistically significant sex differences. Mean Mind-Eat, MEDAS, MEDLIFE and ORTO-15 scores were 3.22 ± 0.46, 8.81 ± 2.35, 3.06 ± 1.14 and 38.31 ± 5.94, respectively. Men scored significantly higher than women in all categories, except for the MEDAS score, where no sex-related differences were observed. Interestingly, the Mind-Eat score was positively associated with ORTO-15 (r = 0.24, p < 0.0001), MEDAS (r = 0.23, p < 0.0001), and MEDLIFE (r = 0.11, p = 0.01) scores. Three clusters were derived, distinguishing optimal (cluster 2), intermediate (cluster 1) and poor (cluster 3) eating profiles, showing sex differences. BMI was significantly lower in cluster 2 than in cluster 3. Conclusions: Our results suggest that integrating psychological and dietary indicators of eating behavior may help identify young adults with less favorable weight-related profiles. Full article

Introduction: Breast milk is the best food for a growing infant during the first 6 months of life. The presence of trans fatty acids (TFAs) in breast milk can interfere with the synthesis of long-chain polyunsaturated fatty acids (LC-PUFAs), increasing the risk of developing, among other issues, asthma or atopic dermatitis. TFAs are not synthesised de novo in the human body. Their content in breast milk may be a good marker of short-term dietary intake of these compounds by breastfeeding mothers. However, the literature shows differences in the assessment of the relationship between dietary TFAs intake and TFAs content in breast milk. Furthermore, the decrease in the TFAs content in food observed recently seems to make it impossible to use Craig-Schmidt’s formula to estimate the TFAs content in the diet or in breast milk. The aim of this study was to confirm the possibility of using TFAs content in breast milk as a marker of their short-term intake within the breastfeeding mother’s diet, together with an attempt at preliminary quantitative determination of the relationship between these parameters. Materials: The study material was collected from a single breastfeeding mother and included 10 breast milk samples and 10 samples of daily food rations reconstructed based on the 24 h food consumption survey. Methods: The content of fatty acids, including TFAs, was determined by gas chromatography–mass spectrometry (GC-MS). Results: The TFAs content in the whole-day mother’s diet and in 100 mL of breast milk ranged from 0.11 to 0.54 g/day and from 0.02 to 0.07 g, respectively. A strong statistically significant (p < 0.05) positive correlation between these parameters was found. Equations for an exploratory linear relationship between the TFAs content in a breastfeeding mother’s diet and the concentration of these fatty acids in breast milk have been developed. Due to the small number of samples, these data should be interpreted very cautiously and validated in a larger cohort. Conclusions: This single-participant pilot study suggests that TFAs content in breast milk may be a marker of the dietary intake of these compounds from the previous day. It seems that the dietary habits of breastfeeding mothers of twins are a significant factor influencing the composition of breast milk and, consequently, the nutritional quality of breastfed infants. Full article

Background: Consumption of ultra-processed foods (UPFs) continues to rise alongside a growing body of epidemiological evidence linking high UPF intake to adverse health outcomes, including cardiovascular disease and type 2 diabetes, in the general population. However, the factors underlying these associations remain incompletely understood, underscoring the need to examine components beyond traditional nutrient composition. In particular, food-processing additives are increasingly recognized as defining features of industrially formulated UPFs. Objective/Methods: In this study, we used a large food label database to cross-sectionally examine the presence and co-occurrence of selected additives (sorbates, benzoates, phosphate additives, and non-nutritive sweeteners [NNSs]) in sweetened beverages sold by the 25 top-selling U.S. food and beverage manufacturers in 2020. Results: We found that sweetened beverages marketed in the U.S. frequently contain multiple additive classes concurrently, supporting the concept that these products represent complex chemical exposure mixtures rather than simple combinations of water and sweeteners. Formulations containing multiple additives were substantially more common than simpler formulations, with many beverages simultaneously containing combinations of sweeteners, preservatives, and phosphate additives. Products containing NNS exhibited higher additive clustering compared to products containing added sugar. Conclusions: Collectively, these findings support the need for broader consideration of beverage formulation complexity in nutrition research, dietary guidance, and policy regulation. Full article

Sustainable agricultural development requires regional agricultural systems to balance output growth, resource efficiency, ecological protection, and long-term resilience. In mountainous and ecologically sensitive regions, identifying the development constraints and statistically derived marginal contribution thresholds of agriculture is essential for promoting green transformation and sustainable land use. Taking Yunnan Province, China, as a representative plateau mountainous agricultural region, this study uses provincial annual data from 1990 to 2023 to quantitatively identify the key drivers and threshold characteristics of agricultural development under multidimensional constraints. A multidimensional indicator system was constructed covering fiscal and investment support, agricultural production inputs, rural infrastructure, and labor and population conditions. Ridge regression was employed to address multicollinearity among explanatory variables, Bootstrap approximate inference was used to improve the robustness of coefficient estimation, and the SHAP interpretation framework was introduced to rank key driving factors and identify marginal contribution thresholds. By integrating ridge regression, Bootstrap approximate inference, SHAP-based contribution ranking, and threshold identification, the proposed framework advances prior agricultural sustainability studies by linking coefficient-based factor analysis with interpretable marginal contribution thresholds under conditions of high multicollinearity and multidimensional resource constraints. The results show that agricultural development in Yunnan is characterized by multidimensional resource and infrastructure constraints. Rural electricity consumption, total reservoir storage capacity, fixed asset investment in agriculture, forestry, animal husbandry and fisheries, local public fiscal budget expenditure, and agricultural population generally act as positive supporting factors. Rural electricity consumption is the most stable and core driver across the aggregate and three sectoral models. In contrast, pesticide and fertilizer inputs show significant negative associations in most models, suggesting that future agricultural development in Yunnan is unlikely to be sustainably supported by continued expansion of high-intensity chemical inputs. Sectoral heterogeneity is also evident: agriculture and animal husbandry are more dependent on energy, water resources, and mechanization, whereas forestry shows a more distinct operational structure. The SHAP dependence analysis identifies several statistically derived marginal contribution thresholds, including rural electricity consumption of approximately 6.055 billion kWh, total reservoir storage capacity of approximately 10.395 billion m³, total agricultural machinery power of approximately 19.8324 million kW, pesticide use of approximately 37,500 tons, and fertilizer application of approximately 1.5238 million tons. These values should be interpreted as empirical transition points in the modeled marginal contributions rather than definitive biophysical ecological limits. They indicate that the sustainability-related constraint structure of agricultural development in Yunnan is not a single output ceiling but a composite interval shaped by infrastructure support capacity, factor allocation conditions, and the declining marginal contribution of high-intensity chemical inputs. The findings provide directional quantitative evidence for sustainable agricultural governance, agricultural green transformation, and differentiated policy discussion in mountainous agricultural regions and offer reference implications for advancing SDG 2 and SDG 15 through the coordination of food-related production, resource use efficiency, and ecosystem conservation. The identified thresholds should be interpreted as model-derived marginal contribution transition points rather than operational policy cutoffs or directly enforceable ecological standards. Full article

Background/Objectives: Celiac disease (CD) is a complex multifactorial disorder driven by genetic susceptibility and environmental triggers, with ultra-processed foods (UPFs) acting as potential disruptors of immune homeostasis. This study aimed to characterize the patterns and temporality of UPF consumption in a pediatric population with CD to provide evidence-based insights that can optimize the nutritional quality of a gluten-free diet (GFD) beyond mere gluten avoidance. Methods: A total of 128 children aged 5–14 years were enrolled, comprising a baseline cohort of 48 children newly diagnosed with CD (pre-GFD), 88 patients who had followed a GFD for at least 6 months (post-GFD), including 44 participants from the pre-GFD cohort prospectively re-evaluated after 12 months and 44 additional patients with established GFD adherence and a control group of 36 healthy children (CTRL). Dietary intake was assessed using three-day 24 h recalls. Food processing levels were determined using the NOVA classification system, and adherence to the Mediterranean Diet was evaluated via the KIDMED index. Results: At baseline, UPFs (NOVA 4) were the primary daily energy source for both celiac patients and controls, accounting for over 57% of total caloric intake, peaking during breakfast (~74%) and afternoon snacks (~81%). Longitudinal analysis showed that the nutritional profile and global UPF consumption remained remarkably stable after 12 months on a GFD, though a significant increase in vitamin B6 intake was observed (0.9 ± 0.4 vs. 1.1 ± 0.5 mg; p = 0.034). However, meal-pattern shifts occurred over the 12 months: celiac children significantly reduced their daily intake of culinary ingredients (NOVA 2; p = 0.029) and processed foods (NOVA 3; p = 0.025). Compared to healthy controls, post-GFD patients exhibited significantly lower Vitamin D intakes (4.6 ± 9.4 vs. 6.2 ± 12.3 µg/day; p = 0.008), meeting only 30.8% of the reference intake. Both groups presented inadequate intakes of iron, calcium, folate, magnesium, and zinc. Conclusions: Pediatric celiac patients exhibit a high, deeply ingrained consumption of UPFs that mirrors healthy controls and persists 12 months after starting a GFD. While the GFD alters meal processing dynamics, it fails to resolve baseline micronutrient insufficiencies and is associated with lower dietary vitamin D intake, highlighting the urgent need for targeted nutritional interventions that focus on whole food quality rather than just gluten elimination. Full article

Pregnancy involves profound metabolic, hormonal, and immunological adaptations essential for fetal development; however, disturbances may lead to complications such as preeclampsia (PE), pre-gestational diabetes, and gestational diabetes mellitus (GDM). These conditions are closely linked to oxidative stress, inflammation, endothelial dysfunction, impaired placentation, and metabolic dysregulation. Consequently, dietary strategies capable of modulating these pathways are of increasing interest. Edible and medicinal mushrooms are widely studied as functional food due to the content of bioactive compounds with antioxidant, anti-inflammatory, immunomodulatory, and metabolic regulatory effects. This review summarizes the nutritional composition of mushrooms and highlights key bioactive constituents with antioxidant and metabolic regulatory properties. Among them, ergothioneine has emerged as a key molecule due to its potent redox-buffering capacity and its potential involvement in the activation of the Nrf2 signaling pathway, a master regulator of cellular antioxidant defense. Through modulation of Nrf2-dependent gene expression, mushroom-derived compounds may contribute to improved cellular resilience against oxidative damage relevant to PE and GDM pathophysiology. Mushroom consumption has additionally been associated with improved glycemic control and enhanced antioxidant defenses in experimental and limited clinical studies, although evidence regarding the prevention or management of hypertensive and metabolic pregnancy complications remains insufficient. Although preclinical findings are promising, clinical evidence remains limited. Further well-designed prospective studies and randomized controlled trials are required to determine efficacy, safety, optimal intake, and active compounds responsible for these effects. Nevertheless, current evidence supports the biological plausibility that edible and medicinal mushrooms are promising dietary modulators of the ergothioneine–Nrf2 axis with potential relevance for maternal–fetal health. Full article

Agri-food systems are increasingly exposed to environmental, economic, and social challenges, including climate change, biodiversity loss, resource depletion, and growing territorial inequalities. In this context, agroecology is increasingly recognised as a transformative paradigm integrating ecological, economic, social, cultural, and political dimensions within broader processes of food-system transition. Within the PRIMA AgrEcoMed project, 24 Italian agroecological initiatives led by women and young farmers were analysed to explore their contribution to agroecological transition processes in Mediterranean rural areas. The study adopts a qualitative multiple-case study approach and evaluates the selected initiatives through the framework of the 13 Principles of Agroecology proposed by the High-Level Panel of Experts on Food Security and Nutrition, organised into three operational axes: improving resource efficiency, strengthening resilience, and ensuring social responsibility and fairness. The results show that the analysed initiatives combine ecological farming practices with processes of multifunctionality, territorial networking, knowledge co-creation, short supply chains, and community engagement. The findings suggest that several initiatives move beyond input-reduction strategies associated with “weak agroecology” and display characteristics consistent with stronger agroecological pathways based on territorial embeddedness, collective learning, and the reorganisation of relationships between production, consumption, and local communities. The paper highlights the relevance of agroecology not only as an environmentally sustainable farming approach, but also as a broader socio-ecological and territorial transition process, as well as the importance of policy frameworks to support territorial agroecological systems. Full article

Within the food industry, research on mobile gastronomy has increased from the consumer perspective. Food trucks play an important role as economic units worldwide, serving as a culinary alternative to traditional restaurants. They have emerged as an innovative initiative and business model that offers a disruptive alternative to home cooked meals. One of the aspects most appreciated by consumers is the charcoal-grilled food offered by food trucks. Globally, charcoal is widely used as an energy source and cooking fuel, with an annual production of approximately 53.2 million tons. Its characteristics and low cost make charcoal a dominant energy resource, and it plays a fundamental role in cooking in both low- and high-income countries due to the distinctive flavor and texture it imparts to food. Research has focused on air pollution and health risks, supplemented with information on the types of charcoal, characteristics and properties, production techniques, and added value. Charcoal ash residue production has not been fully analyzed, providing an opportunity for research to obtain data and evaluate various criteria, such as kilograms of charcoal purchased and food trucks’ residual charcoal ash. To address this gap, the authors propose a horizontal collaboration perspective through the application of the COHRV model to (1) collect data and create a database from food-truck business owners in Ciudad Juarez, Chihuahua; (2) develop a circular economy model for charcoal ash as a sustainable strategy within the food industry; and (3) estimate charcoal consumption during the grilling process and the generation of charcoal ash residue in the food truck sector. Full article

Background/Objectives: Childhood and adolescence are critical periods for bone mineral accrual and future skeletal reserve. Milk intake, sun exposure and caffeinated energy drink consumption are familiar lifestyle concepts, but they differ substantially in biological proximity and evidential strength. This structured narrative review critically evaluates these exposures in relation to peak bone mass accrual in youth. Methods: PubMed/MEDLINE, Scopus, Web of Science, the Cochrane Library and Google Scholar were searched from database inception to 23 June 2026. Search terms combined pediatric population terms with bone outcomes and exposure terms related to milk/dairy, calcium, vitamin D, sun exposure, physical activity, sleep, caffeine and energy drinks. A literature collection flowchart and a GRADE-informed evidence appraisal table are provided to improve transparency and clinical interpretability. Results: Evidence is strongest for adequate calcium intake, calcium-rich foods and weight-bearing physical activity as modifiable contributors to skeletal accrual. Vitamin D is essential for mineral homeostasis, but supplementation effects on bone density in otherwise healthy children are context-dependent and appear most relevant for deficiency prevention or treatment. Milk intake is best interpreted as a practical marker of calcium-rich dietary patterns rather than as the only route to calcium adequacy. Sun exposure is an indirect determinant of vitamin D status and is modified by season, latitude, skin pigmentation, clothing, sunscreen, adiposity and outdoor behavior. Direct evidence linking caffeinated energy drinks to impaired pediatric bone accrual is very limited. The relevance of caffeinated energy drink intake is better framed as indirect and hypothesis-generating, through possible displacement of calcium-rich beverages, sleep disruption and clustering with poorer lifestyle patterns. Conclusions: A prevention framework for pediatric bone health should emphasize calcium adequacy, avoidance of vitamin D deficiency, mechanical loading and correct pediatric DXA interpretation using Z-scores. Energy drinks can be included as a lifestyle concern, but conclusions should remain cautious because direct skeletal evidence is limited. Full article

Hepatitis E virus (HEV) is an emerging zoonotic pathogen in Europe, mainly transmitted via consumption of naturally contaminated food or contact with infected animals. People living in the Balkans have diverse dietary habits, with high pork consumption in some countries, making this region a relevant setting for investigating HEV seroprevalence and its possible determinants. The current study aimed to estimate pooled HEV seroprevalence among adults in the general population and blood donors and to assess factors associated with regional variation. Twenty-eight eligible studies were identified from PubMed, Scopus, and Web of Science following the PRISMA guidelines. Pooled prevalence estimates were calculated using a random-effects meta-analysis of proportions implemented via a generalized linear mixed model (GLMM) with logit transformation. Potential factors associated with HEV seroprevalence, including national pork consumption, serological assay type, population group, year of publication, sex, and country, were evaluated. The pooled anti-HEV seroprevalence was estimated to be 5.68% (95% CI: 3.48–9.12%), with substantial heterogeneity. Country-specific estimates ranged from 1.01% in Greece to 26.66% in Bulgaria. Subgroup analyses showed significant variation according to national pork consumption category, serological assay type, year of publication, and country. However, meta-regression indicated that methodological and temporal factors, particularly serological assay type and year of publication, were the main significant moderators, whereas national pork consumption was not independently associated with seropositivity. Therefore, pork consumption should be interpreted as an exploratory ecological indicator rather than as evidence of a direct association. The methodological differences contribute substantially to the variability in HEV seroprevalence across the Balkans, emphasizing the need for standardized diagnostic approaches within a One Health framework. Full article