Search Results (1,251)

Background: Local anesthesia is essential for most dental procedures, but its parenteral administration is often painful. Topical anesthetics are commonly used to minimize local anesthesia pain; however, commercial formulations fail to fully prevent the discomfort of local anesthetic injection. Methods: We developed and characterized a novel lidocaine and epinephrine co-loaded liquid crystalline precursor system (LCPS) for topical anesthesia. The formulation was structurally characterized using polarized light microscopy (PLM) and small-angle X-ray scattering (SAXS). Rheological behavior was assessed through continuous and oscillatory rheological analyses. Texture profile analysis, in vitro mucoadhesive force evaluation, in vitro drug release and permeation studies, and an in vivo toxicity assay using the chicken chorioallantoic membrane (CAM) model were also conducted. Results: PLM and SAXS confirmed the transition of the LCPS from a microemulsion to a lamellar liquid crystalline structure upon contact with artificial saliva. This transition enhanced formulation consistency by over 100 times and tripled mucoadhesion strength. The LCPS also provided controlled drug release, reducing permeation flow by 93% compared to the commercial formulation. Importantly, the CAM assay indicated that the LCPS exhibited similar toxicity to the commercial product. Conclusions: The developed LCPS demonstrated promising physicochemical and biological properties for topical anesthesia, including enhanced mucoadhesion, controlled drug delivery, and acceptable biocompatibility. These findings support its potential for in vivo application and future clinical use to reduce pain during dental anesthesia procedures. Full article

Kiwifruit (Actinidia spp.) is a globally important economic fruit with high nutritional value. Fruit peelability, defined as the mechanical ease of separating the peel from the fruit flesh, is a critical quality trait influencing consumer experience and market competitiveness and has emerged as a critical breeding target in fruit crop improvement programs. The present review systematically synthesized existing studies on kiwifruit peelability, and focused on its evolutionary trajectory, genotypic divergence, quantitative evaluation, possible underlying mechanisms, and artificial manipulation strategies. Kiwifruit peelability research has advanced from early exploratory studies in New Zealand (2010s) to systematic investigations in China (2020s), with milestones including the development of evaluation metrics and the identification of genetic resources. Genotypic variation exists among kiwifruit genera. Several Actinidia eriantha accessions and the novel Actinidia longicarpa cultivar ‘Guifei’ exhibit superior peelability, whereas most commercial Actinidia chinensis and Actinidia deliciosa cultivars exhibit poor peelability. Quantitative evaluation highlights the need for standardized metrics, with “skin-flesh adhesion force” and “peel toughness” proposed as robust, instrument-quantifiable indicators to minimize operational variability. Mechanistically, peelability is speculated to be governed by cell wall polysaccharide metabolism and phytohormone signaling networks. Pectin degradation and differential distribution during fruit development form critical “peeling zones”, whereas ethylene, abscisic acid, and indoleacetic acid may regulate cell wall remodeling and softening, collectively influencing skin-flesh adhesion. Owing to the scarcity of easy-to-peel kiwifruit cultivars, artificial manipulation methods, including manual peeling benchmarking, lye treatment, and thermal peeling, can be employed to further optimize kiwifruit peelability. Currently, shortcomings include incomplete genotype-phenotype characterization, limited availability of easy-peeling germplasms, and a fragmented understanding of the underlying mechanisms. Future research should focus on methodological innovation, germplasm development, and the elucidation of relevant mechanisms. Full article

Aim: Pacifiers play a critical role in the early stages of craniofacial and palate development during infancy. While they provide comfort and aid in soothing, their use can also have significant impacts on the growth and function of the oral cavity. This study aimed to simulate and predict the behavior of six different types of pacifiers and their functional interaction with the tongue and palate, with the goal of understanding their potential effects on orofacial growth and development. Materials and Methods: Biomechanical analysis using Finite Element Analysis (FEA) mathematical models was employed to evaluate the behavior of six different commercial pacifiers in contact with the palate and tongue. Three-dimensional solid models of the palate and tongue were based on the mathematical framework from a 2007 publication. This allowed for a detailed investigation into how various pacifier designs interact with soft and hard oral tissues, particularly the implications on dental and skeletal development. Results: The findings of this study demonstrate that pacifiers exhibit different interactions with the oral cavity depending on their geometry. Anatomical–functional pacifiers, for instance, tend to exert lateral compressions near the palatine vault, which can influence the hard palate and contribute to changes in craniofacial growth. In contrast, other pacifiers apply compressive forces primarily in the anterior region of the palate, particularly in the premaxilla area. Furthermore, the deformation of the tongue varied significantly across different pacifier types: while some pacifiers caused the tongue to flatten, others allowed it to adapt more favorably by assuming a concave shape. These variations highlight the importance of selecting a pacifier that aligns with the natural development of both soft and hard oral tissues. Conclusions: The results of this study underscore the crucial role of pacifier geometry in shaping both the palate and the tongue. These findings suggest that pacifiers have a significant influence not only on facial bone growth but also on the stimulation of oral functions such as suction and feeding. The geometry of the pacifier affects the soft tissues (tongue and muscles) and hard tissues (palate and jaw) differently, which emphasizes the need for careful selection of pacifiers during infancy. Choosing the right pacifier is essential to avoid potential negative effects on craniofacial development and to ensure that the benefits of proper oral function are maintained. Therefore, healthcare professionals and parents should consider these biomechanical factors when introducing pacifiers to newborns. Full article

Complete mitochondrial DNA genome annotation of an ecologically and commercially important fish species Cirrhinus cirrhosus was executed with next-generation sequencing (NGS) for nucleotide and phylogenetic analyses. The findings of this study showed that the Cirrhinus cirrhosus mitochondrial genome contained 16,593 bp, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 tRNA genes, and a D-loop region. The overall base composition was 32% adenine, 25% thiamine, 16% guanine, and 27% cytosine. This mitochondrial DNA exhibits an AT biasness, with 56% AT content in its genome. Significant fluctuations were identified in the AT and GC skew values of the ND6 gene, indicating that the selection and mutation forces acting on this gene might be different from those acting on other genes. The Ka/Ks ratios of most protein-coding genes were less than 1, indicating very strong natural selection pressure. Phylogenetic analysis of Cirrhinus cirrhosus with Cirrhinus mrigala and Bangana tungting suggested a closer evolutionary relationship among these species, which might have shared a more recent common ancestor. It has been also found that the genera Labeo and Cirrhinus are not monophyletic. Full article

Wearable pressure sensors have emerged as vital tools in personalized monitoring, promising transformative advances in patient care and diagnostics. Nevertheless, conventional devices frequently suffer from limited sensitivity, inadequate flexibility, and concerns regarding biocompatibility. Herein, we introduce silk fibroin, a naturally occurring protein extracted from silkworm cocoons, as a promising material platform for next-generation wearable sensors. Owing to its remarkable biocompatibility, mechanical robustness, and structural tunability, silk fibroin serves as an ideal substrate for constructing capacitive pressure sensors tailored to medical applications. We engineered silk-derived capacitive architecture and evaluated its performance in real-time human motion and physiological signal detection. The resulting sensor exhibits a high sensitivity of 18.68 kPa⁻¹ over a broad operational range of 0 to 2.4 kPa, enabling accurate tracking of subtle pressures associated with pulse, respiration, and joint articulation. Under extreme loading conditions, our silk fibroin sensor demonstrated superior stability and accuracy compared to a commercial resistive counterpart (FlexiForce™ A401). These findings establish silk fibroin as a versatile, practical candidate for wearable pressure sensing and pave the way for advanced biocompatible devices in healthcare monitoring. Full article

The development of bio-based adhesives as sustainable alternatives to synthetic formulations presents a significant opportunity for advancing environmental sustainability in packaging applications. This research aimed to develop and evaluate a bio-based adhesive derived from bacterial nanocellulose (BNC), aloe vera and its mixtures as a potential replacement for commercial synthetic adhesives. Aloe vera, selected for its polysaccharide-rich composition, served as a natural polymeric matrix, while BNC contributed reinforcing properties. The adhesive formulations, with and without BNC, were compared to a commercial adhesive to assess their mechanical performance. T-peel and shear tests were conducted on smooth and rough paper substrates to evaluate adhesive strength. The bio-based adhesive incorporating BNC demonstrated superior shear and peel strength on rough substrates due to enhanced mechanical interlocking within the fibrous structure of paper, whereas performance on smooth surfaces was hindered by uneven BNC distribution, reducing adhesive-substrate interaction. Although the commercial adhesive achieved higher absolute maximum force values, the bio-based formulation exhibited comparable mechanical stability under specific conditions. These findings underscore the influence of substrate properties and application methods on adhesive performance, highlighting the potential of bio-based adhesives in packaging applications and the need for further formulation optimization to fully realize their advantages over traditional synthetic adhesives. Full article

Background/Objectives: The forced oscillation technique (FOT) is a non-invasive, effort-independent method for assessing respiratory mechanics and is particularly suited for young children who cannot reliably perform spirometry. This study aimed to evaluate the main anthropometric determinants of respiratory impedance parameters—resistance (Rrs) and reactance (Xrs)—in healthy Bulgarian children aged 2 to 8 years. Methods: A total of 100 healthy children were evaluated using a commercially available device at oscillation frequencies of 5, 11, and 19 Hz. Anthropometric data were collected, and FOT measurements were conducted following ATS/ERS guidelines. Stepwise multiple linear regression was applied to identify predictors of Rrs and Xrs. Results: Height (mean height of the children: 113.89 ± 8.46 cm) emerged as the most significant determinant of both Rrs and Xrs across all frequencies with a moderate inverse correlation observed between Rrs at 5 Hz and height (r = −0.446; p < 0.001). Weight additionally influenced reactance at 5 Hz. The mean R5–19 was 0.55, but no significant associations with height or weight were found. Stepwise regression confirmed height as the sole consistent predictor, while sex and age had no significant effect. Conclusions: This study is the first to present the determinants of key FOT parameters in a population of Bulgarian children. Height was identified as the strongest predictor of respiratory impedance and should be prioritized in the development of reference values for children under 8 years old. These findings reinforce the clinical utility of FOT in early childhood. Full article

Modern fly-by-wire (FBW) aircraft demand high-fidelity simulation systems for research and training, yet existing force-sensing solutions are often prohibitively expensive. This study presents the design, development, and validation of a low-cost, reconfigurable force-sensing sidestick. The system utilizes four strain-gauge load cells to capture pure pilot force inputs, integrated with a 6-DoF non-linear flight model. To evaluate its performance, a pitch-angle tracking task was conducted with 16 participants (pilots and non-pilots). Objective metrics revealed that the control strategy was a primary determinant of performance. Participants employing a proactive feedforward control strategy exhibited roughly an order of magnitude lower tracking-error variance than those relying on reactive corrections. Subjective assessments using the Cooper-Harper scale and NASA-TLX corroborated the objective data, confirming the sidestick’s ability to differentiate control techniques. This work demonstrates an open-source platform that makes high-fidelity FBW simulation accessible for academic research, pilot training, and human factors analysis at a fraction of the cost of commercial systems. Full article

Lumped plasticity models available in commercial software offer a limited resolution of damage distribution along structural members. This study presents an open-source workflow that combines force-based fiber elements in OpenSeesPy with automated 3D post-processing for visualizing distributed plasticity in reinforced concrete piles. A 60 cm diameter pile subjected to monotonic lateral loading is analyzed using both SAP2000’s default plastic hinges and OpenSeesPy fiber sections (Concrete02/Steel02). Although the fiber model incurs a runtime approximately 2.5 times greater, it captures the gradual spread of yielding and deterioration with improved fidelity. The presented workflow includes Python routines for interactive stress–strain visualization, facilitating the identification of critical sections and verification of strain limits. This approach offers a computationally feasible alternative for performance-based analysis with enhanced insight into member-level behavior. Because the entire workflow—from model definition through post-processing—is fully scripted in Python, any change to geometry, materials, or loading can be re-run in seconds, dramatically reducing the time taken to execute sensitivity analyses. Full article

The commercialization of selective broccoli harvesters, a critical response to agricultural labor shortages, is hampered by end effectors with large operational envelopes and poor adaptability to complex field conditions. To address these limitations, this study developed and evaluated a novel end-effector with an integrated transverse cutting mechanism and a foldable grasping cavity. Unlike conventional fixed cylindrical cavities, our design utilizes actuated grasping arms and a mechanical linkage system to significantly reduce the operational footprint and enhance maneuverability. Key design parameters were optimized based on broccoli morphological data and experimental measurements of the maximum stem cutting force. Furthermore, dynamic simulations were employed to validate the operational trajectory and ensure interference-free motion. Field tests demonstrated an operational success rate of 93.33% and a cutting success rate of 92.86%. The end effector successfully operated in dense planting environments, effectively avoiding interference with adjacent broccoli heads. This research provides a robust and promising solution that advances the automation of broccoli harvesting, paving the way for the commercial adoption of robotic harvesting technologies. Full article

The focus on selecting broilers for rapid growth rates and enhanced breast muscle yield has resulted in a decline in meat quality. The differences in carcass characteristics and meat quality between Hubbard white broilers (HWs, a commercial breed) and Xueshan chickens (XSs, an indigenous breed) at market age were analyzed to determine the potential mechanisms responsible for these differences. The results show that HWs exhibited significantly better carcass performance than XSs, including the larger weight of the carcass, the breast muscle, and the thigh muscle (p < 0.01). In addition, based on HE staining analysis, HWs’ breast muscles had a considerably larger average myofiber area and diameter than those of XSs (p < 0.01). Furthermore, the physical characteristics of the meat revealed that XSs had higher redness and yellowness and also higher lightness. HW meat had a higher pH and thermal loss, but a lower shear force and drip loss than XS meat (p < 0.01). The content of saturated fatty acids (SFAs) and polyunsaturated fatty acids (PUFAs) was, remarkably, lower in the breast muscles of HWs than of XSs (p < 0.01). In contrast, HWs had a larger concentration of monounsaturated fatty acids (MUFAs) than XSs (p < 0.01). Finally, the breast muscles of XSs had lower levels of mRNA expression for genes linked to lipid metabolism, such as fatty acid binding protein 4 (Fabp4) and peroxisome proliferator-activated receptor alpha (Pparα), and had higher levels of the phosphofructokinase muscle type (Pfkm) compared to HWs (p < 0.01). These results indicate that a lower carcass yield was observed in XSs compared with HWs, but that XSs showed better performance in terms of meat quality than HW. Full article

With the development and application of electric vehicles powered by lithium-ion batteries (LIBs) at high altitude, the lack of research on the aging laws and mechanisms of LIBs under a low-pressure aviation environment has become an important obstacle to their safe application. Herein, the influences and mechanisms of high-altitude and low-pressure environment (50 kPa) on the cycling performance of commercial pouch LIBs were systematically studied. The results showed that low air pressure caused a sharp decrease in battery capacity to 46.6% after 200 cycles, with a significant increase in charge transfer impedance by 70%, and the contribution rate of active lithium loss reached 74%. Low air pressure led to irreversible deformation of the battery, resulting in the expansion of the gap between the electrodes, poor electrolyte infiltration, and reduction of the effective lithium insertion area, which in turn induced multiple synergistic accelerated decay mechanisms, including obstructed lithium-ion transmission, reduced interfacial reaction efficiency, increased active lithium consumption, changes in heat generation structure, and a significant increase in heat generation. After applying external force, the deformation of the electrode was effectively suppressed, and the cycle capacity retention rate increased to 87.6%, which significantly alleviated the performance degradation of LIBs in low pressure environment. This work provides a key theoretical basis and engineering solutions for the design of power batteries in high-altitude areas. Full article

Genetic resistance is a sustainable way to protect crops from diseases, and breeding resistant varieties is a key objective. However, diseases are caused by pathogens with different life cycles, and the importance of individual evolutionary forces plays a key role in the adaptation of their populations. Therefore, strategies for the use of genetic resistance resources can vary depending on the plant pathosystem. Numerous major genes confer hypersensitive resistance to powdery mildew—one of the most common diseases in barley—but these genes conform to the gene-for-gene system of an extremely diverse and adaptable pathogen. When such resistance genes are transferred into commercial varieties, their efficiency in the field is soon overcome and replacement with newly developed resistant varieties can be slow. Hence, specific resistance genes should not be used in barley breeding programs. Only one monogenic, non-hypersensitive, non-specific and durable major resistance Mlo is known. This predominates in Central and Western European spring varieties and should be widely adopted by barley breeders elsewhere and in other crops where such type of resistance is found. In this paper, the relevant aspects involved in breeding barley resistant to powdery mildew are discussed, with conclusions supported by practical examples. Additionally, future directions for barley improvement are proposed. Full article

This paper proposes a multibody model calibration method for vehicle misuse testing. During misuse tests conducted at high driving speeds, the vehicle’s responses can become highly nonlinear due to certain key model parameters. Direct calibration using a complex multibody model is time-consuming and unstable, as it may fail or diverge due to improper settings of the model parameters. Therefore, a modified quarter-vehicle model is proposed for the analytical calibration of these nonlinear parameters by introducing an additional constraint on the sprung mass to recover the restoring force. The new model features only two degrees of freedom and incorporates key nonlinear parameters, including the suspension’s stiffness and the wheel’s center mass. It is suitable for misuse tests involving tire detachment at high driving speeds. The detailed analytical calibration procedure for the nonlinear parameters is deduced and subsequently validated through numerical simulation using these parameters. When the parameters are sufficiently close to the actual ones or linearly related to the responses, an optimization method such as the least squares method can be applied, along with simulations using complex models in commercial software. Full article

Electric-assist bicycles have recently become very popular. However, riding them generally requires significantly more energy, generated simultaneously by the motor and the rider, compared to much lighter traditional bicycles. Assessing the energy efficiency of electric-assist bicycles in comparison to traditional bikes allows us to determine in which cases using electric bikes is cost-effective and in which it is not. This study proposes a method for evaluating the energy efficiency of bicycles, which stands out by relying on relatively imprecise data recorded at low frequency by a commercial bike computer with accessories. The core of the method is an algorithm developed by the authors to determine the tractive force acting on the bicycle and rider, based on a minimal set of recorded data: road incline, riding speed, and the wind speed component parallel to the direction of movement. Depending on the situation, the tractive force may act as a driving force or as a braking force. Based on the calculated tractive force, the power required to maintain the recorded bicycle speed can be estimated. Full article