Search Results (441)

Hydrogels are widely known for their ability to increase soil water retention and for their potential slow nutrient release mechanism. They have been constantly improved to meet the growing demand for sustainability in agriculture. Research focused on the development of biodegradable hydrogels, produced from industrial cellulose waste, are an ecological and efficient alternative soil ameliorant for the improvement of agricultural land. The objective of this study was to evaluate the impacts of two types of hydrogel (processed in a glass reactor versus a twin-screw extruder) on soils with different textures (clay and sandy loam), testing their water retention capacity, nitrogen leaching, and effects on seed germination. The methodology included the evaluation of water retention capacity at different pressures with different hydrogel addition rates in the soil, leaching tests in columns filled with soil and hydrogel layers, and germination tests of sorghum and corn. The results indicated that the addition of hydrogel significantly improved water retention, especially in sandy loam soils. The hydrogels also reduced nitrogen leaching, acting as nitrification inhibitors and limiting the conversion of ammonium to nitrate, with greater effectiveness in clayey soils. In the tested formulations, it was observed that the hydrogel doses applied to the columns favored nitrogen retention in the region close to the roots, directly influencing the initial stages of germination. This behavior highlights the potential of hydrogels as tools for directing nutrients in the soil profile, indicating that adjustments to the C:N ratio, nutrient release rate, and applied doses can optimize their application for different crops. Full article

The performance of reinforced concrete (RC) frame structures will gradually decrease over time, posing a threat to the safety of buildings. Although the performance of some buildings may still meet the safety requirements, they cannot meet new usage requirements. Therefore, this paper proposes a new-type joint to promote the development of research on the reinforcement and renovation of RC frame structures in response to this situation. The RC beams and columns of the joints are connected by embedded horizontal steel plate (a single plate with dimension of 150 mm × 200 mm × 5 mm), and the beams and columns are individually wrapped in steel. Through conducting low cyclic loading tests, this paper analyzes the influence of carrying out wrapped steel treatment and the thickness of wrapped steel of the beam and connector on mechanical performance indicators such as hysteresis curve, skeleton curve, stiffness, ductility, and energy dissipation. The experimental results indicate that the reinforcement using steel plate can significantly improve the dynamic performance of the joint. The effect of changing the thickness of the connector on the dynamic performance of the specimen is not significant, while increasing the thickness of wrapped steel of beam can effectively improve the overall strength of joint. The research results of this paper will help promote the application of reinforcement and renovation technology for existing buildings, and improve the quality of human living. Full article

The separation of three proton pump inhibitors (omeprazole, lansoprazole, and rabeprazole) as exemplified molecules containing chiral sulfoxide groups was investigated in polar organic liquid chromatographic mode on seven different polysaccharide stationary phases (Chiralcel OD and OJ; Chiralpak AD, AS, and IA; Lux Cellulose-2 and -4). Different alcohols, such as methanol, ethanol, 1-propanol, 2-propanol, and their combinations, were used as eluents. After method optimization, semi-preparative enantioseparation was successfully applied for the three proton pump inhibitors to collect the individual enantiomers. A detailed investigation was conducted into elution order reversal, thermodynamic parameters, the effect of eluent mixtures, and the hysteresis of retention time and selectivity. Using Chiralpak AS, containing the amylose tris[(S)-α-methylbenzylcarbamate] chiral selector, the separation of the investigated enantiomers was achieved in all four neat eluents, with methanol providing the best results. In many cases, a reversal of the enantiomer elution order was observed. In addition to chiral-selector-dependent reversal, eluent-dependent reversal was also observed. Notably, even replacing methanol with ethanol altered the enantiomer elution order. Both enthalpy- and entropy-controlled enantioseparation were also observed in several cases; however, temperature-dependent elution order reversal was not. The hysteresis of retention and selectivity was further investigated on amylose-type columns in methanol–2-propanol and methanol–ethanol eluent mixtures. The phenomenon was observed on all amylose columns regardless of the eluent mixtures employed. Hystereticity ratios were calculated and used to compare the hysteresis behaviors of different systems. Multivariate statistical analysis revealed that Chiralpak AS exhibited the most distinct enantioselective behavior among the tested columns, likely due to the absence of a direct connection between the carbamate moiety and the aromatic substituent. The present study aided in understanding the mechanisms leading to enantiomer recognition, which is crucial for developing new chiral stationary phases and chiral HPLC method development in general. Full article

A modified activated carbon (AC) was developed by modifying with poly(diallyldimethylammonium) chloride (PDADMAC) to enhance its adsorption performance for water treatment applications. Different PDADMAC concentrations were explored and evaluated using methyl red as a model contaminant, with 8 w/v% PDADMAC yielding the best adsorption performance. The kinetics data were well described by the pseudo-first-order equation and homogeneous surface diffusion model. The Freundlich isotherm fit the equilibrium data well, indicating multilayer adsorption and diverse interaction types. The removal efficiency remained similar across a pH range of 5–9 and in the presence of background inorganic (NaCl)/organic compounds (sodium acetate) at different concentrations. Rapid small-scale column tests were performed to simulate continuous flow conditions, and the PDADMAC-modified AC effectively delayed the breakthrough of the contaminant compared to raw AC. Regeneration experiments showed that 0.1 M NaOH with 70% methanol effectively restored the adsorption capacity, retaining 80% of the initial efficiency after five cycles. Quantum chemical analysis revealed that non-covalent interactions, including electrostatic and Van der Waals forces, governed the adsorption mechanism. Overall, the results of this study prove that PDADMAC-AC shows great potential for enhanced organic contaminant removal in water treatment systems. Full article

Since farmers in the inland valley region of the Niger Delta mostly rely on experience rather than empirical evidence when it comes to irrigation, flood irrigation being the most popular technique, the region’s agricultural sector needs more efficient water management. In order to better understand the intricate hydrodynamics of water flow through the soil subsurface, this study aimed to develop a soil column laboratory experimental setup for soil water infiltration. The objective was to measure the soil water content and soil matric potential at 10 cm intervals to study the soil water characteristic curve as a relationship between the two hydraulic parameters, mimicking drip soil subsurface micro-irrigation. A specially designed cylindrical vertical soil column rig was built, and an EQ3 equitensiometer of Delta-T Devices was used in the laboratory as a precision sensor to measure the soil matric potential Ψ (kPa), and the volumetric soil water content θ (%) was measured using a WET150 sensor of Delta-T Devices. The relationship between the volumetric soil water content and the soil matric potential resulted in the generation of the soil water characteristic curve. Two separate monoliths of undisturbed soil samples from Ivrogbo and Oleh in the Nigerian inland valley of the Niger Delta, as well as a uniformly packed sample of soil from Aberdeen, UK, for comparison, were used in gravity-driven flow experiments. In each case, tests were performed once on the monoliths of undisturbed soil samples. In contrast, the packed sample was subjected to an experiment before being further agitated to simulate ploughing and then subjected to an infiltration experiment, resulting in a total of four samples. The Van Genuchten model of the soil water characteristic curve was used for the verification of the experimental results. Comparing the four samples’ volumetric soil water contents and soil matric potentials at various depths revealed a significant variation in their behaviour. However, compared to the predicted curve, the range of values was narrower. Compared to n = 2 in the Van Genuchten curve, the value of n at 200 mm depth was found to be 15, with θr of 0.046 and θs of 0.23 for the packed soil sample, resulting in a percentage difference of 86.7%. Additionally, n = 10 for the ploughed sample resulted in an 80% difference, yet θr = 0.03 and θs = 0.23. For the Ivrogbo sample and the Oleh sample, the range of the matric potential was relatively too small for the comparison. The pre-experiment moisture content of the soil samples was part of the cause of this, in addition to differences in the soil types. Furthermore, the data revealed a remarkable agreement between the measured behaviour and the projected technique of the soil water characteristic curve. Full article

The use of both structural steel and reinforced concrete is common in civil and military infrastructure projects. Anchorage plays a crucial role in these systems, serving as the key element that connects structural components and secures attachments within complex composite structures. This research focuses on evaluating the performance of steel–concrete column connections under the combined effects of lateral loading and fire exposure. Additionally, the study investigates the use of carbon fiber-reinforced polymers (CFRP) for strengthening and repairing these connections. The research methodology combines experimental testing and finite-element modeling to achieve its objectives. First, experimental investigation was carried out to test two groups of steel-reinforced concrete column specimens, each group made of three specimens. The first group specimens were designed based on special moment frame (SMF) detailing, and the other group specimens were designed based on intermediate moment frame (IMF) detailing. These two types of design were selected based on seismic demands, with SMFs offering high ductility and resilience for severe earthquakes and IMFs providing a cost-effective solution for moderate seismic zones, both benefiting from ongoing innovations in connection detailing and design approaches. Then, finite-element analysis was conducted to model the test specimens. High-fidelity finite-element modeling was conducted using ANSYS program, which included three-dimensional coupled thermal-stress analyses for the six tested specimens and incorporated nonlinear temperature-dependent materials characteristics of each component and the interfaces. Both the experimental and numerical results of this study show that fire has a more noticeable effect on displacement compared to the peak capacities of both types of specimens. Fire exposure results in a larger reduction in the initial residual lateral stiffness of the SMF specimens when compared to IMF specimens. While the effect of CFRP wraps on initial residual lateral stiffness was consistent for all specimens, it caused more improvement for the IMF specimen in terms of post-fire ductility when compared to SMF specimens. This exploratory study confirms the need for further research on the effect of fire on the concrete–steel anchorage zones. Full article

Fibronectin (FN), a primary component of the extracellular matrix (ECM), features multiple structural domains closely linked to various cellular behaviors, including migration, spreading, adhesion, and proliferation. The FN3 domain, which contains the RGD sequence, is critical in tissue repair because it enables interaction with integrin receptors on the cell surface. However, the large molecular weight of wild-type FN presents challenges for its large-scale production through heterologous expression. Therefore, this study focused on cloning the FN3 functional domain of full-length FN for expression and validation. This study selected Bacillus subtilis as the expression host due to its prominent advantages, including efficient protein secretion, absence of endotoxins, and minimal codon bias. The recombinant vector pHT43-FN3 was successfully constructed through homologous recombination technology and transformed into Bacillus subtilis WB800N. The FN3 protein was successfully expressed after induction with IPTG. Following purification of the recombinant FN protein using a His-tag nickel column, SDS-PAGE analysis showed that the molecular weight of FN3 was approximately 27.3 kDa. Western blot analysis confirmed the correct expression of FN3, and the BCA protein assay kit determined a protein yield of 5.4 mg/L. CCK8 testing demonstrated the good biocompatibility of FN3. In vitro cell experiments showed that FN3 significantly promoted cell migration at a 20 μg/mL concentration and enhanced cell adhesion at 10 μg/mL. In summary, this study successfully utilized Bacillus subtilis to express the FN3 functional domain peptide from FN protein and has validated its ability to promote cell migration and adhesion. These findings not only provide a strategy for the expression of FN protein in B. subtilis, but also establish an experimental foundation for the potential application of FN3 protein in tissue repair fields such as cutaneous wound healing and cartilage regeneration. Full article

This paper presents the physical, hydrogeological, and geochemical characterizations of two types of tailings: one from the nickel–cobalt (Ni–Co) and the other from the lead–zinc (Pb–Zn) industries. The study is restricted only to Ni and Zn ions behavior. The mineralogical composition of the studied tailings is primarily composed of oxides and hydroxides of iron, aluminum, and silica. Based on their grain size, these wastes are geotechnically classified as low plasticity silts, with permeability ranging from 10⁻⁸ m/s to less than 10⁻⁹ m/s. Batch and column flow tests, along with metal transport tests using heavy metal-contaminated wastewater, reveal that these tailings have an adsorption capacity for metals such as nickel (Ni) and zinc (Zn) ranging from 2000 to 6000 mg/kg of solid. This high adsorption capacity surpasses that of many clayey soils used for sealing municipal, industrial, mining, and metallurgical waste deposits. Additionally, these wastes can neutralize the acidity of wastewater. The results indicate that the mineralogical composition and pH of these tailings are key factors determining their adsorption characteristics and mechanisms. Due to their characteristics, these tailings could be evaluated for use as low-permeability reactive geochemical barriers (LPRGB) in the conditioning of repositories for the storage of industrial, urban, mining and metallurgical waste. This would allow large volumes of tailings to be repurposed effectively. Full article

Bolt connections are the primary component of beam–column joints, which frequently become loose during their service life due to environmental factors. Assessing the tightness of bolts is essential for maintaining structural integrity and safety. Although the guided wave method has been proven effective for detecting bolt looseness, the severe dispersion properties and complex structure of beam–column joints pose difficulties for the quantitative evaluation of bolt looseness. Therefore, a deep neural network model integrating a convolutional neural network (CNN), long short-term memory (LSTM), and multi-head self-attention mechanism (MHSA) is introduced to identify the degree of looseness in multiple bolts using SH-typed guided waves. The dispersion properties of the I-shaped steel beam were analyzed using the semi-analytical finite element method, and a mode weight coefficient was presented to clarify the mode distribution under different types of external loads. Two pairs of transducers arranged on the same side of the bolt-connected region were utilized to obtain the directly incoming and end-reflected wave packets from four wave propagation paths. The received signals were converted into time–frequency spectra, and the effective components were extracted to form the input pattern for the neural network. Numerical simulations were performed on a beam–column joint with eight bolts, and the number of training samples was increased using data augmentation techniques. The results indicate that the CNN-LSTM-MHSA model can accurately estimate the bolt looseness conditions better than other methods. Noise injection testing was also conducted to investigate the effect of measurement noise. Full article

Understanding the complex phenomena of interactions between the elements of a combined piled raft foundation (CPRF) is essential for the proper design of such foundations. To evaluate the effects of mutual influence among the CPRF’s elements, a series of long-term measurements of selected physical quantities related to the performance of the foundation were conducted on a building with a frame structure, stiffening walls, and monolithic technology, consisting of seven aboveground stories and one underground story. The analysis distinguishes the real deformations resulting from temperature changes and from stress strains resulting from load changes. The two types of deformations were subjected to further interpretation of only changes in the stress and strain over time. Changes in stress values in the subsoil, as well as strain measurements in the vertical direction of concrete columns, were recorded to assess the load distribution between the CPRF’s components. The numerical analysis results obtained for a fragment of the monitored foundation were compared with actual measurement results to verify the numerical model of interaction between the structure and the soil. Field monitoring and FEA methods were used to compare the long-term deformation analysis, and they helped to minimize the monitoring time. This comparison also served to supplement and simultaneously expand the dataset of test results on a real-world scale. Full article

This article presents the design process for a modern stage trapdoor, which was designed to optimize the work of cultural facilities personnel and increase the attractiveness of future performances and events. Strength calculations for the supporting structure were carried out in the Soldis DESIGNER program, and based on these, a 3D model of the stage trapdoor was designed and placed in the space of the stage chimney. In order to verify and analyze the strength of the structure, the 3D model was prepared for detailed analysis in the Autodesk Inventor program. Tests were carried out for four load cases of the structure for 15 different load values. Information about the maximum value of the deflection arrow and the maximum stress was obtained. Collected data were organized in tables and displayed in line and column charts, based on which conclusions were drawn. These analyses showed a high degree of compliance between calculations from both programs. It was found that in this type of structure, a detailed analysis in 3D CAD programs is not necessary for the proper design of the supporting structure, which allows for simplification of the design process. The designed trapdoor meets all design requirements and can be implemented as a solution to improve the functionality and aesthetics of the stage’s technical equipment. Full article

Objective: This study aimed to develop a robust multi-residue analytical method for the precise identification and quantification of six macrolide antiparasitic agents commonly used in animal husbandry feeds. Method: Feed samples were extracted using a water-saturated acetonitrile solution. The resulting crude extracts were then treated with n-hexane and further purified by HLB solid-phase extraction columns to obtain the test solutions. These prepared samples were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The method was validated across six different feed matrices, including pig premix, concentrate, and complete feed, as well as chicken premix, concentrate, and compound feed. The method exhibited average recoveries ranging from 80.07% to 98.80%. The intra-day coefficients of variation (CV) for the first three feed types ranged from 1.98% to 12.84%, while for the latter three, the CVs ranged from 2.43% to 13.69%. Results: The method’s precision led to the quantification limit of avermectin, doramectin, acetyl avermectin, and ivermectin being 25 μg/kg, whereas for moxifloxacin and milbemycin, the limit was 50 μg/kg. These thresholds meet the stringent requirements for trace drug analysis, supporting the method’s suitability for regulatory surveillance and monitoring of these specified antibiotics in animal feeds. Full article

Concrete-filled steel columns are increasingly recognised for their enhanced structural performance. This study investigates an innovative shear connector design with screw connectors as an alternative to conventional connection types. From push-out testing, the shear capacity of screw connectors in composite columns comprising cold-formed steel sigma sections and concrete infill was evaluated. Experimental push-out testing demonstrated the effectiveness of theoretical equations in estimating the shear strength of screw connections. The comparison indicates that established design methods provide reasonable predictions, supporting their applicability in practical scenarios. Theoretical equations in the literature for estimating shear strength were tested for suitability and gave comparable results. Disassembling of tested specimens showed that a concrete failure was the prominent mode of ultimate condition. Shear screws offer a novel design alternative to conventional shear connection methods. They demonstrate significant potential for structural applications when integrated with advanced composite column sections, such as the four-sigma built-up CFS sections. The study highlights screw connectors as a cost-effective, sustainable, and practical solution for innovative composite column designs, offering significant potential for construction and maintenance efficiency. Full article

To enhance the seismic performance of existing reinforced concrete (RC) columns, this study proposes a novel strengthening method that combines steel strips with ultra–high–performance concrete (UHPC). The seismic behavior of the proposed method is investigated through quasi–static cyclic tests conducted on four strengthened columns and one control column. The experimental parameters include the type of reinforcement (UHPC–only and UHPC combined with steel strips) and the thickness of the UHPC strengthening layer. The failure modes, hysteretic behavior, energy dissipation capacity, and stiffness degradation of the specimens are systematically analyzed. The results show that, compared to the unstrengthened column, the UHPC–strengthened columns achieved maximum increases of 73.73% in peak load and 23.68% in ductility coefficient, while the columns strengthened with composite steel strips achieved further improvements of up to 84.79% and 50.23%, respectively. The composite strengthening method significantly improved the failure mode, with crack distribution changing from localized crushing to multiple fine cracks. The displacement ductility coefficient reached as high as 6.28, and the hysteretic curve fullness and cumulative energy dissipation increased by a factor of two to three. Finally, based on moment equilibrium theory, a theoretical formula is proposed to calculate the lateral ultimate flexural capacity of RC columns strengthened with steel strip–UHPC composites, which shows good agreement with the experimental results. Full article

As a key load-bearing component in building structures, the effective strengthening of reinforced concrete (RC) columns is critical to enhancing their structural reliability and service life. To tackle the issue of excessive self-weight from the increasing section strengthening method and further optimize the seismic performance of encased steel strengthening, this paper presents a novel composite strengthening method for RC columns, which is characterized by using Lightweight Alkali-Activated Slag Concrete (LAASC) as the strengthening layer and an X-type encased steel structure. By conducting axial compression tests on six columns and utilizing in-depth research on small eccentric compression and hysteresis performance through numerical simulation, the specific effects of different strengthening materials and encased steel forms on the mechanical properties of the columns are systematically explored. Experimental results indicate that compared to ordinary concrete strengthening layers, LAASC can reduce the self-weight of the strengthening layer by 25%, boost the bearing capacity of the strengthened components by 37%, and enhance the vertical deformation capacity by 100%. Numerical simulation also confirms that X-type encased steel composite strengthening can effectively control bending deformation under small eccentric compression, reducing lateral deflection by 30–35% compared to un-strengthened columns. Under horizontal reciprocating loading, the cumulative energy dissipation of X-type encased steel composite-strengthened columns is 15–30% higher than that of traditional steel encased composite-strengthened columns, reflecting the diagonal bracing effect of the X-type batten plates. Full article