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Keywords = cold-chain foods and packaging

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21 pages, 1621 KB  
Article
Sustainability-Oriented Digital–Green Cold-Chain Logistics Investment: A Readiness–Intensity CRITIC–CoCoSo Assessment of Chinese Provinces
by Ende Feng, Qiyue Wang and Tao Yu
Sustainability 2026, 18(16), 8459; https://doi.org/10.3390/su18168459 - 18 Aug 2026
Abstract
Provincial cold-chain investment decisions must reconcile food-loss prevention, digital visibility, logistics capability and the environmental burden of freight-intensive growth. This study develops a sustainability-oriented readiness–intensity framework for 31 provincial-level regions in mainland China. The baseline model uses 14 auditable public-data criteria and combines [...] Read more.
Provincial cold-chain investment decisions must reconcile food-loss prevention, digital visibility, logistics capability and the environmental burden of freight-intensive growth. This study develops a sustainability-oriented readiness–intensity framework for 31 provincial-level regions in mainland China. The baseline model uses 14 auditable public-data criteria and combines Criteria Importance Through Intercriteria Correlation (CRITIC) with the standard Combined Compromise Solution (CoCoSo) algorithm. Because the observations combine 2024 statistics, a 2023 digital-finance index and the cumulative 2020–2025 cold-chain-base list, the design is described as an asynchronous cross-sectional snapshot rather than a single-year panel. Municipal sewage and green-space variables are interpreted as regional enabling capacity, not direct cold-chain environmental performance; road freight turnover relative to gross domestic product is treated as a cost-type freight-intensity transition-pressure proxy. A separate diagnostic replaces the earlier inverse-size term with logistics residuals conditional on agri-food output. Shandong, Guangdong, Jiangsu, Henan and Zhejiang form the leading demonstration-readiness group. Equal-weight CoCoSo closely matches the CRITIC result (Spearman ρ = 0.996), while TOPSIS and VIKOR retain the broad ordering but expose local method sensitivity. Dropping either digital criterion, removing the three indirect green proxies, winsorizing the normalization range, varying the CoCoSo compromise parameter and substituting 2022 digital data do not alter the leading pattern. Under an assumed 5% indicator-error perturbation, Shandong and Guangdong remain within ranks 1–2, whereas the ordering of several adjacent provinces is less secure. The framework supports sequenced investment packages rather than a deterministic league table and distinguishes demonstration-ready, scale-led, intensity-led and coverage-building contexts. Full article
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27 pages, 1952 KB  
Review
Smart Adsorption-Based Nanocatalysts for Active Food Packaging: A Critical Look at the Gap Between Concept and Application
by Amir Khojastehnezhad, Maziar Jafari, Fatemeh S. Mohseni-Shahri, Farid Moeinpour and Mohamed Siaj
Nanomaterials 2026, 16(16), 980; https://doi.org/10.3390/nano16160980 - 10 Aug 2026
Viewed by 226
Abstract
Conventional food packaging cannot actively regulate spoilage-related molecules such as ethylene and volatile organic compounds that accumulate inside sealed packages. Smart adsorption based nanocatalysts (SABNs) integrate adsorptive scaffolds, catalytic centers, and stimuli responsive triggers to progressively remove these spoilage markers. This review establishes [...] Read more.
Conventional food packaging cannot actively regulate spoilage-related molecules such as ethylene and volatile organic compounds that accumulate inside sealed packages. Smart adsorption based nanocatalysts (SABNs) integrate adsorptive scaffolds, catalytic centers, and stimuli responsive triggers to progressively remove these spoilage markers. This review establishes a unified three pillar framework and critically examines how adsorption, catalytic degradation, and regeneration cycles are proposed to function under food-relevant conditions. Across major food categories, reported photocatalytic systems achieve ethylene removal efficiencies of 50% to 90% and extend shelf life by 1 to 5 days under controlled light and temperature. However, performance declines sharply under the dark, humid, and refrigerated conditions typical of real supply chains. A systematic evidence level grading of twelve representative SABN systems reveals that the majority cluster at levels L3 and L4, while none has yet reached level L5, which requires both standardized migration testing and sensory evaluation. Key barriers, including nanoparticle migration, fragmented regulation, scalability, and life-cycle impacts, are assessed. By introducing explicit inclusion/exclusion criteria and a six-level evidence grading framework, this review maps critical gaps in migration data and cold-chain validation and outlines a staged roadmap toward regulation-ready active packaging technologies. Full article
(This article belongs to the Section Nanocomposite Materials)
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29 pages, 1449 KB  
Review
Innovative Preservation Technologies and Supply Chain Optimization for Reducing Meat Loss and Waste: Current Advances, Challenges, and Future Perspectives
by Hysen Bytyqi, Ana Novo Barros, Victoria Krauter, Slim Smaoui and Theodoros Varzakas
Sustainability 2026, 18(1), 530; https://doi.org/10.3390/su18010530 - 5 Jan 2026
Cited by 5 | Viewed by 3210 | Correction
Abstract
Food loss and waste (FLW) is a chronic problem across food systems worldwide, with meat being one of the most resource-intensive and perishable categories. The perishable character of meat, combined with complex cold chain requirements and consumer behavior, makes the sector particularly sensitive [...] Read more.
Food loss and waste (FLW) is a chronic problem across food systems worldwide, with meat being one of the most resource-intensive and perishable categories. The perishable character of meat, combined with complex cold chain requirements and consumer behavior, makes the sector particularly sensitive to inefficiencies and loss across all stages from production to consumption. This review synthesizes the latest advancements in new preservation technologies and supply chain efficiency strategies to minimize meat wastage and also outlines current challenges and future directions. New preservation technologies, such as high-pressure processing, cold plasma, pulsed electric fields, and modified atmosphere packaging, have substantial potential to extend shelf life while preserving nutritional and sensory quality. Active and intelligent packaging, bio-preservatives, and nanomaterials act as complementary solutions to enhance safety and quality control. At the same time, blockchain, IoT sensors, AI, and predictive analytics-driven digitalization of the supply chain are opening new opportunities in traceability, demand forecasting, and cold chain management. Nevertheless, regulatory uncertainty, high capital investment requirements, heterogeneity among meat types, and consumer hesitancy towards novel technologies remain significant barriers. Furthermore, the scalability of advanced solutions is limited in emerging nations due to digital inequalities. Convergent approaches that combine technical innovation with policy harmonization, stakeholder capacity building, and consumer education are essential to address these challenges. System-level strategies based on circular economy principles can further reduce meat loss and waste, while enabling by-product valorization and improving climate resilience. By integrating preservation innovations and digital tools within the framework of UN Sustainable Development Goal 12.3, the meat sector can make meaningful progress towards sustainable food systems, improved food safety, and enhanced environmental outcomes. Full article
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36 pages, 2031 KB  
Review
Pre- and Postharvest Determinants, Technological Innovations and By-Product Valorization in Berry Crops: A Comprehensive and Critical Review
by Elsa M. Gonçalves, Rui Ganhão and Joaquina Pinheiro
Horticulturae 2026, 12(1), 19; https://doi.org/10.3390/horticulturae12010019 - 24 Dec 2025
Cited by 7 | Viewed by 3394
Abstract
Berries—including strawberries, blueberries, raspberries, blackberries, cranberries, and several less commonly cultivated berry species—are highly valued for their sensory quality and rich content of bioactive compounds, yet they are among the most perishable horticultural products. Their soft texture, high respiration rate, and susceptibility to [...] Read more.
Berries—including strawberries, blueberries, raspberries, blackberries, cranberries, and several less commonly cultivated berry species—are highly valued for their sensory quality and rich content of bioactive compounds, yet they are among the most perishable horticultural products. Their soft texture, high respiration rate, and susceptibility to fungal pathogens lead to rapid postharvest deterioration and significant economic losses. This review synthesizes advances in berry postharvest management reported between 2010 and 2025. Conventional strategies such as rapid precooling, cold-chain optimization, controlled and modified atmospheres, and edible coatings are discussed alongside emerging non-thermal technologies, including UV-C light, ozone, cold plasma, ultrasound, biocontrol agents, and intelligent packaging systems. Particular emphasis is placed on the instability of anthocyanins and other phenolic compounds, microbial spoilage dynamics, and the influence of cultivar genetics and preharvest factors on postharvest performance. The review also highlights opportunities for circular-economy applications, as berry pomace, seeds, and skins represent valuable sources of polyphenols, dietary fiber, and seed oils for use in food, nutraceutical, cosmetic, and bio-based packaging sectors. Looking ahead, future research should prioritize integrated, multi-hurdle, low-residue postharvest strategies, the scale-up of non-thermal technologies, and data-driven cold-chain management. Overall, coordinated physiological, technological, and sustainability-oriented approaches are essential to maintain berry quality, reduce postharvest losses, and strengthen the resilience of berry value chains. Full article
(This article belongs to the Special Issue Postharvest Physiology and Quality Improvement of Fruit Crops)
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9 pages, 1124 KB  
Proceeding Paper
From Harvest to Market: Postharvest Technologies for Reducing Waste and Enhancing Food Security
by Ashra Khadim Hussain, Saddam Hussain, Mubashra Khadim Hussain, Madiha Javed and Rana Muhammad Aadil
Biol. Life Sci. Forum 2025, 51(1), 7; https://doi.org/10.3390/blsf2025051007 - 23 Dec 2025
Cited by 1 | Viewed by 3528
Abstract
Postharvest technologies and supply chain management are critical to improving food security, reducing losses, and advancing sustainability in global agri-food systems. Nearly one-third of global food is lost after harvest, particularly in developing regions, underscoring the urgent need for efficient postharvest handling, cold [...] Read more.
Postharvest technologies and supply chain management are critical to improving food security, reducing losses, and advancing sustainability in global agri-food systems. Nearly one-third of global food is lost after harvest, particularly in developing regions, underscoring the urgent need for efficient postharvest handling, cold chain integration, and sustainable logistics systems. This paper explores key components of effective postharvest systems, including harvesting, treatments, storage, and value-added processing. It highlights digital innovations IoT sensors, blockchain, AI, and digital twins that enhance traceability, forecasting, and operational efficiency. Case studies from South Asia, Africa, Europe, and North America emphasize region-specific solutions, highlighting low-cost technologies for smallholders and advanced systems for export chains. Sustainable practices such as renewable-powered cold chains, circular economy models, and eco-friendly packaging align with Sustainable Development Goals (SDGs) on zero hunger, responsible consumption, and climate action. This paper concludes that while technology is vital, systemic transformation requires inclusive policies and collaboration among governments, private sectors, researchers, and farming communities to build resilient, equitable food systems. Full article
(This article belongs to the Proceedings of The 9th International Horticulture Conference & Expo)
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18 pages, 559 KB  
Review
Sustainable Postharvest Innovations for Fruits and Vegetables: A Comprehensive Review
by Valeria Rizzo
Foods 2025, 14(24), 4334; https://doi.org/10.3390/foods14244334 - 16 Dec 2025
Cited by 8 | Viewed by 4176
Abstract
The global food industry is undergoing a critical shift toward sustainability, driven by high postharvest losses—reaching up to 40% for fruits and vegetables—and the need to reduce environmental impact. Sustainable postharvest innovations focus on improving quality, extending shelf life, and minimizing waste through [...] Read more.
The global food industry is undergoing a critical shift toward sustainability, driven by high postharvest losses—reaching up to 40% for fruits and vegetables—and the need to reduce environmental impact. Sustainable postharvest innovations focus on improving quality, extending shelf life, and minimizing waste through eco-efficient technologies. Advances in non-thermal and minimal processing, including ultrasound, pulsed electric fields, and edible coatings, support nutrient preservation and food safety while reducing energy consumption. Although integrated postharvest technologies can reduce deterioration and microbial spoilage by 70–92%, significant challenges remain, including global losses of 20–40% and the high implementation costs of certain nanostructured materials. Simultaneously, eco-friendly packaging solutions based on biodegradable biopolymers and bio-composites are replacing petroleum-based plastics and enabling intelligent systems capable of monitoring freshness and detecting spoilage. Energy-efficient storage, smart sensors, and optimized cold-chain logistics further contribute to product integrity across distribution networks. In parallel, the circular bioeconomy promotes the valorization of agro-food by-products through the recovery of bioactive compounds with antioxidant and anti-inflammatory benefits. Together, these integrated strategies represent a promising pathway toward reducing postharvest losses, supporting food security, and building a resilient, environmentally responsible fresh produce system. Full article
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42 pages, 1850 KB  
Review
Date Palm (Phoenix dactylifera L.) Fruit: Strategic Crop for Food Security, Nutritional Benefits, Postharvest Quality, and Valorization into Emerging Functional Products
by Nasser Al-Habsi
Sustainability 2025, 17(16), 7491; https://doi.org/10.3390/su17167491 - 19 Aug 2025
Cited by 26 | Viewed by 11361
Abstract
Date palm (Phoenix dactylifera L.) is a vital crop cultivated primarily in developing regions, playing a strategic role in global food security through its significant contribution to nutrition, economy, and livelihoods. Global and regional production trends revealed increasing demand and expanded cultivation [...] Read more.
Date palm (Phoenix dactylifera L.) is a vital crop cultivated primarily in developing regions, playing a strategic role in global food security through its significant contribution to nutrition, economy, and livelihoods. Global and regional production trends revealed increasing demand and expanded cultivation areas, underpinning the fruit’s importance in national food security policies and economic frameworks. The date fruit’s rich nutritional profile, encompassing carbohydrates, dietary fiber, minerals, and bioactive compounds, supports its status as a functional food with health benefits. Postharvest technologies and quality preservation strategies, including temperature-controlled storage, advanced drying, edible coatings, and emerging AI-driven monitoring systems, are critical to reducing losses and maintaining quality across diverse cultivars and maturity stages. Processing techniques such as drying, irradiation, and cold plasma distinctly influence sugar composition, texture, polyphenol retention, and sensory acceptance, with cultivar- and stage-specific responses guiding optimization efforts. The cold chain and innovative packaging solutions, including vacuum and modified atmosphere packaging, along with biopolymer-based edible coatings, enhance storage efficiency and microbial safety, though economic and practical constraints remain, especially for smallholders. Microbial contamination, a major challenge in date fruit storage and export, is addressed through integrated preservation approaches combining thermal, non-thermal, and biopreservative treatment. However, gaps in microbial safety data, mycotoxin evaluation, and regulatory harmonization hinder broader application. Date fruit derivatives such as flesh, syrup, seeds, press cake, pomace, and vinegar offer versatile functional roles across food systems. They improve nutritional value, sensory qualities, and shelf life in bakery, dairy, meat, and beverage products while supporting sustainable waste valorization. Emerging secondary derivatives like powders and extracts further expand the potential for clean-label, health-promoting applications. This comprehensive review underscores the need for multidisciplinary research and development to advance sustainable production, postharvest management, and value-added utilization of date palm fruits, fostering enhanced food security, economic benefits, and consumer health worldwide. Full article
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11 pages, 1235 KB  
Article
Foodborne Botulism Caused by Clostridium botulinum Subtype A5(b3) by Self-Packaged Vacuum Spicy Rabbit Heads
by Wen Cui, Chuanmin Ma, Ming Liu, Yan Li, Lin Zhou, Yuwen Shi, Xuefang Xu and Hui Liu
Microorganisms 2025, 13(7), 1662; https://doi.org/10.3390/microorganisms13071662 - 15 Jul 2025
Cited by 1 | Viewed by 4092
Abstract
Botulism is a severe muscle paralysis disease mediated by the botulinum toxin. Here, we reported a foodborne botulism case caused by Clostridium botulinum subtype A5(b3) from self-packaged vacuum spicy rabbit heads. Treatment for this case was delayed due to misdiagnosis and insufficient diagnostic [...] Read more.
Botulism is a severe muscle paralysis disease mediated by the botulinum toxin. Here, we reported a foodborne botulism case caused by Clostridium botulinum subtype A5(b3) from self-packaged vacuum spicy rabbit heads. Treatment for this case was delayed due to misdiagnosis and insufficient diagnostic capacity in three hospitals, which resulted in progressive clinical deterioration, and eventually, the patient was transferred to Shandong Public Health Clinical Center for specialized therapy. The case was suspected as foodborne botulism by the Qilu Medical-Prevention Innovation Integration pathway and multi-disciplinary consultation. An epidemiological investigation and laboratory confirmation revealed that the botulinum neurotoxin originated from vacuum-packaged spicy rabbit heads distributed via interprovincial cold chain logistics. After treatment with botulism antiserum, the patient’s condition significantly improved, and they were discharged after recovery. We revealed that this foodborne botulism outbreak was caused by the Clostridium botulinum A5(b3) subtype from food by whole-genome sequencing and SNP typing. All the strains belonged to Group I carrying the botulinum neurotoxin gene classified as the ha cluster. Toxin A was confirmed by MBA and other methods, while toxin B was non-functional due to the truncated bont/B gene. Other virulence genes and antibiotic resistance genes were also detected. Our findings indicate that self-packaged vacuum meat products represent an emerging risk factor for botulism transmission when stored improperly. Importantly, the recurrent misdiagnosis in this case underscored the urgent need to enhance the training of healthcare professionals in medical institutions to improve the diagnostic accuracy and clinical management of botulism. Full article
(This article belongs to the Special Issue Feature Papers in Food Microbiology)
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21 pages, 5601 KB  
Article
Effect of Low-Temperature Plasma Sterilization on the Quality of Pre-Prepared Tomato-Stewed Beef Brisket During Storage: Microorganism, Freshness, Protein Oxidation and Flavor Characteristics
by Qihan Shi, Ying Xiao, Yiming Zhou, Jinhong Wu, Xiaoli Zhou, Yanping Chen and Xiaodan Liu
Foods 2025, 14(7), 1106; https://doi.org/10.3390/foods14071106 - 22 Mar 2025
Cited by 5 | Viewed by 2405
Abstract
Traditional tomato-braised beef brisket with potatoes is celebrated for its rich, complex flavors and culinary appeal but requires lengthy preparation. Pre-packaged versions of the dish rely on thermal sterilization for safety; however, high-temperature processing accelerates protein and lipid oxidation, thereby compromising its sensory [...] Read more.
Traditional tomato-braised beef brisket with potatoes is celebrated for its rich, complex flavors and culinary appeal but requires lengthy preparation. Pre-packaged versions of the dish rely on thermal sterilization for safety; however, high-temperature processing accelerates protein and lipid oxidation, thereby compromising its sensory quality. As the demand for ready-to-eat meals grows, the food industry faces the challenge of ensuring microbial safety while preserving flavor integrity. In this study, low-temperature plasma sterilization (LTPS) (160 KV, 450 s) was evaluated as a non-thermal alternative to conventional high-temperature short-time (HSS) sterilization. Furthermore, a comprehensive analysis was conducted over a 10-day storage period, assessing microbial viability, physicochemical properties (pH, shear force, and water-holding capacity), oxidative markers (TBARS, TVB-N, and protein carbonyls), volatile compounds (GC-MS), and electronic nose (e-nose) responses. The results revealed that LTPS (160 kV, 450 s) successfully maintained bacterial counts below regulatory limits (5 lg CFU/g) for 72 h, ensuring that the microbial indicators of short-term processed products sold to supermarkets through cold chain logistics were in the safety range. Additionally, LTPS-treated samples showed a 4.2% higher water-holding capacity (p < 0.05) during storage, indicating improved preservation of texture. Furthermore, LTPS-treated samples exhibited 32% lower lipid oxidation (p < 0.05) and retained 18% higher sulfhydryl content (p < 0.05) compared to HSS, indicating reduced protein oxidation. GC-MS and e-nose analyses showed that LTPS preserved aldehydes and ketones associated with meaty aromas, while HSS contributed to sulfur-like off-flavors. Principal component analysis showed that the LTPS samples had shorter distances across various storage periods compared to HSS, indicating reduced differences in aroma difference. The findings of this study demonstrate LTPS’s dual efficacy in microbial control and aroma preservation. The technology presents a viable strategy for extending the shelf life of pre-prepared meat dishes while reducing oxidative and flavor deterioration, thereby establishing a solid foundation for LTPS application in the pre-prepared food sector. Full article
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25 pages, 8231 KB  
Article
Quality Changes in Live Ruditapes philippinarum During “Last Mile” Cold Chain Breakage: Effect of Packaging
by Yiming Huang, Xinrui Xie, Shoaib Younas, Caiyun Liu and Xin Wang
Foods 2025, 14(6), 1011; https://doi.org/10.3390/foods14061011 - 17 Mar 2025
Cited by 3 | Viewed by 2207
Abstract
The reliability of the “last mile” of cold-chain logistics is crucial for food safety. This study investigated the effect of different packaging treatments on the quality of anhydrously preserved live Ruditapes philippinarum (R. philippinarum) in “last mile” cold chain disruption. The temperature [...] Read more.
The reliability of the “last mile” of cold-chain logistics is crucial for food safety. This study investigated the effect of different packaging treatments on the quality of anhydrously preserved live Ruditapes philippinarum (R. philippinarum) in “last mile” cold chain disruption. The temperature profiles of three packaging treatments at ambient temperature (25 °C) were monitored. Quality assessment was conducted based on sensory scoring, survival rate, total viable count (TVC), water-holding capacity (WHC), pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid-reactive substances (TBA), color, and texture. Low-frequency nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) were utilized to characterize the water state profile. The findings demonstrated a progressive increase in internal package temperature throughout the “last mile”, with packages containing additional ice packs more effectively maintaining lower temperature and restricting the migration of “hot spots” towards the center. Specifically, the package with three ice packs maintained a markedly lower temperature, which effectively inhibited microbial activity, lipid oxidation, and the production of alkaline substances, resulting in higher survival rates, water-holding capacity, texture, sensory acceptability, and immobilized water fraction. Furthermore, LF-NMR relaxation parameters showed strong correlations with various physicochemical indices, suggesting a potential approach for real-time quality monitoring. This study provides insights for maintaining live R. philippinarum quality during the “last mile”. Full article
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27 pages, 11240 KB  
Article
Investigating the Potential Effects of Food Waste Reduction Interventions Within the Leafy Vegetable Supply Chain in Kermanshah Province, Iran
by Mostafa Moradi, Hossein Shabanali Fami, Ali Akbar Barati, Felicitas Schneider, Lusine Henrik Aramyan and Reza Salehi Mohammadi
Agriculture 2024, 14(12), 2344; https://doi.org/10.3390/agriculture14122344 - 20 Dec 2024
Cited by 1 | Viewed by 2557
Abstract
Despite the increasing concerns regarding meeting the world’s future food demand, there is still a substantial quantity of food loss and waste (FLW), particularly concerning fruits and vegetables. In the case of Kermanshah province, inefficiencies within the leafy vegetable supply chpain (LVSC) contribute [...] Read more.
Despite the increasing concerns regarding meeting the world’s future food demand, there is still a substantial quantity of food loss and waste (FLW), particularly concerning fruits and vegetables. In the case of Kermanshah province, inefficiencies within the leafy vegetable supply chpain (LVSC) contribute to an alarming annual waste of 39% of leafy vegetables. Although several studies have proposed strategies and recommendations for mitigating this waste, the actual impact of these interventions on reducing FLW has not been thoroughly examined or quantified. Using System Dynamic Modeling, this study offers a novel approach to quantify the impact of interventions on waste reduction. The quantification results reveal four key interventions reducing vegetable waste at the production stage: biotic (31.2%) and abiotic stress control (14.4%), improved educational services (23.2%), and access to quality inputs (15.2%). Furthermore, the results suggest that early-stage factors in the LVSC play a crucial role in determining waste accumulation in later stages. Improvements in packaging facilities and cold supply chain infrastructure, along with better coordination and information sharing among stakeholders at the market stage, significantly help reduce waste. Additionally, effective planning for household food shopping is emphasized as a crucial strategy for minimizing waste at the consumption stage. This holistic approach focuses on the interconnectedness of actions across various stages of the supply chain and their combined effect on decreasing the overall waste of leafy vegetables. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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12 pages, 278 KB  
Article
Impact of Refrigerated Storage on Microbial Growth, Color Stability, and pH of Turkey Thigh Muscles
by Agnieszka Orkusz, Giorgia Rampanti, Monika Michalczuk, Martyna Orkusz and Roberta Foligni
Microorganisms 2024, 12(6), 1114; https://doi.org/10.3390/microorganisms12061114 - 30 May 2024
Cited by 33 | Viewed by 5378
Abstract
The quality of poultry meat offered to the consumer depends mainly on the level of hygiene during all stages of its production, storage time, and temperature. This study investigated the effect of refrigerated storage on the microbiological contamination, color, and pH of turkey [...] Read more.
The quality of poultry meat offered to the consumer depends mainly on the level of hygiene during all stages of its production, storage time, and temperature. This study investigated the effect of refrigerated storage on the microbiological contamination, color, and pH of turkey thigh muscles stored at 1 °C over six days. Microbial growth, including total mesophilic aerobes, presumptive lactic acid bacteria, and Enterobacteriaceae, significantly increased, impacting the meat’s sensory attributes and safety. On the 6th day of meat storage, the content of total mesophilic aerobes, presumptive lactic acid bacteria, and Enterobacteriaceae was 1.82 × 107 CFU/g, 1.00 × 104 CFU/g, and 1.87 × 105 CFU/g, respectively. The stability of color was assessed by quantifying the total heme pigments, comparing myoglobin, oxymyoglobin, and metmyoglobin concentrations, analyzing color parameters L*, a*, b*, and the sensory assessment of surface color, showing a decline in total heme pigments, three myoglobin forms, redness (a*) and lightness (L*). In contrast, yellowness (b*) increased. These changes were correlated with the growth of spoilage microorganisms that influenced the meat’s pigmentation and pH, with a notable rise in pH associated with microbial metabolization. Based on the conducted research, it was found that the maximum storage time of turkey thigh muscles at a temperature of 1 °C is 4 days. On the 4th day of storage, the total mesophilic aerobe content was 3.5 × 105 CFU/g. This study underscores the critical need for maintaining controlled refrigeration conditions to mitigate spoilage, ensuring food safety, and preserving turkey meat’s sensory and nutritional qualities. There is a need for further research to improve turkey meat storage techniques under specific temperature conditions by studying the impact of using varying packaging materials (with different barrier properties) or the application of natural preservatives. Additionally, future studies could focus on evaluating the effectiveness of cold chain management practices to ensure the quality and safety of turkey products during storage. By addressing these research gaps, practitioners and researchers can contribute to developing more efficient and sustainable turkey meat supply chains, which may help mitigate food wastage by safeguarding the quality and safety of the meat. Full article
(This article belongs to the Section Food Microbiology)
17 pages, 4073 KB  
Article
The Development of Aptamer-Based Gold Nanoparticle Lateral Flow Test Strips for the Detection of SARS-CoV-2 S Proteins on the Surface of Cold-Chain Food Packaging
by Xiaotong Li, Jiachen Wang, Ge Yang, Xiaona Fang, Lianhui Zhao, Zhaofeng Luo and Yiyang Dong
Molecules 2024, 29(8), 1776; https://doi.org/10.3390/molecules29081776 - 13 Apr 2024
Cited by 17 | Viewed by 6444
Abstract
The COVID-19 pandemic over recent years has shown a great need for the rapid, low-cost, and on-site detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, an aptamer-based colloidal gold nanoparticle lateral flow test strip was well developed to realize [...] Read more.
The COVID-19 pandemic over recent years has shown a great need for the rapid, low-cost, and on-site detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, an aptamer-based colloidal gold nanoparticle lateral flow test strip was well developed to realize the visual detection of wild-type SARS-CoV-2 spike proteins (SPs) and multiple variants. Under the optimal reaction conditions, a low detection limit of SARS-CoV-2 S proteins of 0.68 nM was acquired, and the actual detection recovery was 83.3% to 108.8% for real-world samples. This suggests a potential tool for the prompt detection of SARS-CoV-2 with good sensitivity and accuracy, and a new method for the development of alternative antibody test strips for the detection of other viral targets. Full article
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15 pages, 2534 KB  
Article
New Implications of Metabolites and Free Fatty Acids in Quality Control of Crossbred Wagyu Beef during Wet Aging Cold Storage
by Shuji Ueda, Yuka Yoshida, Biniam Kebede, Chiaki Kitamura, Ryo Sasaki, Masakazu Shinohara, Itsuko Fukuda and Yasuhito Shirai
Metabolites 2024, 14(2), 95; https://doi.org/10.3390/metabo14020095 - 29 Jan 2024
Cited by 11 | Viewed by 4932
Abstract
Efficient cold-chain delivery is essential for maintaining a sustainable global food supply. This study used metabolomic analysis to examine meat quality changes during the “wet aging” of crossbred Wagyu beef during cold storage. The longissimus thoracic (Loin) and adductor muscles (Round) of hybrid [...] Read more.
Efficient cold-chain delivery is essential for maintaining a sustainable global food supply. This study used metabolomic analysis to examine meat quality changes during the “wet aging” of crossbred Wagyu beef during cold storage. The longissimus thoracic (Loin) and adductor muscles (Round) of hybrid Wagyu beef, a cross between the Japanese Black and Holstein–Friesian breeds, were packaged in vacuum film and refrigerated for up to 40 days. Sensory evaluation indicated an increase in the umami and kokumi taste owing to wet aging. Comprehensive analysis using gas chromatography-mass spectrometry identified metabolite changes during wet aging. In the Loin, 94 metabolites increased, and 24 decreased; in the Round, 91 increased and 18 decreased. Metabolites contributing to the umami taste of the meat showed different profiles during wet aging. Glutamic acid increased in a cold storage-dependent manner, whereas creatinine and inosinic acid degraded rapidly even during cold storage. In terms of lipids, wet aging led to an increase in free fatty acids. In particular, linoleic acid, a polyunsaturated fatty acid, increased significantly among the free fatty acids. These results provide new insight into the effects of wet aging on Wagyu-type beef, emphasizing the role of free amino acids, organic acids, and free fatty acids generated during cold storage. Full article
(This article belongs to the Section Food Metabolomics)
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14 pages, 1671 KB  
Article
Evaluation of a Calcium Carbonate-Based Container for Transportation and Storage of Fresh Fish as a Sustainable Alternative to Polystyrene Boxes
by Evgenia Basdeki, Eleni Mpenetou, Polyxeni Papazoglou, Dimitrios Ladakis, Emmanouil Flemetakis, Apostolos Koutinas and Theofania Tsironi
Sustainability 2024, 16(1), 130; https://doi.org/10.3390/su16010130 - 22 Dec 2023
Cited by 7 | Viewed by 4366
Abstract
The present study aimed to assess the effect of alternative packaging materials on the quality retention and shelf-life of whole fish under low and abuse temperature conditions. Red sea bream (Pagrus major) was harvested and stored in different packaging containers, i.e., [...] Read more.
The present study aimed to assess the effect of alternative packaging materials on the quality retention and shelf-life of whole fish under low and abuse temperature conditions. Red sea bream (Pagrus major) was harvested and stored in different packaging containers, i.e., a conventional polystyrene (PS) box, a CaCO3-based box and a cardboard box (tested as a simple alternative container for transportation and short-term storage of food). After harvesting and transportation, fish was stored in the tested containers at 2 °C for 11 days and periodically kept at room temperature (25 °C) to simulate potential temperature fluctuations in the actual supply chain. The effect of temperature fluctuations and packaging materials on the quality and remaining shelf-life of fish was determined by microbial enumeration (total viable counts, Pseudomonas spp. and Enterobacteriaceae). PS retained fish quality and maintained a low temperature of fish for longer periods of time during storage at ambient conditions. The CaCO3-based containers also showed satisfactory performance, resulting in a similar microbial load in fish flesh to the samples stored in PS boxes after 11 days of simulated transportation and storage (TVC load 7.8–8.0 logcfu/g). Cardboard resulted in a rapid increase in the internal temperature during the temperature fluctuations at ambient conditions, resulting in higher microbial loads of fish flesh at all stages of the simulated cold chain. The replacement of conventional plastic packaging materials with alternative, environmentally friendly packaging systems without affecting the shelf-life of fish may reduce plastic waste while ensuring the high quality and shelf-life of perishable food products. Full article
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