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Search Results (1,452)

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Keywords = chloride salt

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22 pages, 2670 KiB  
Review
Sodium Chloride in Food
by Sylwia Chudy, Agnieszka Makowska and Ryszard Kowalski
Foods 2025, 14(15), 2741; https://doi.org/10.3390/foods14152741 - 6 Aug 2025
Abstract
Sodium chloride is a chemical compound that has been encountered by people for thousands of years, and plays a significant role in their lives. The aim of this article is to provide a comprehensive review of table salt from the perspective of health, [...] Read more.
Sodium chloride is a chemical compound that has been encountered by people for thousands of years, and plays a significant role in their lives. The aim of this article is to provide a comprehensive review of table salt from the perspective of health, food technology, and cultural heritage. The article discusses salt extraction and production, its composition and consumption, and its effects on the human body. The authors draw attention to new trends, such as the use of micronized salt, microencapsulated salt, and salt with colors and shapes that differ from those of typical table salt. Scientific studies on the presence of undesirable substances and the use of salt additives were reviewed. The role of salt in dairy, meat, and bakery technology was illustrated. Gaps in research on salt were highlighted. In the last part, all types of salt with geographical indications are shown. The paper suggests that producers with a long tradition in the salt sector should apply for the European geographical indications to enhance their national and cultural heritage and promote their region. The review highlights the need for further research on all aspects discussed. Full article
(This article belongs to the Section Food Quality and Safety)
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36 pages, 10414 KiB  
Article
Forces During the Film Drainage and Detachment of NMC and Spherical Graphite in Particle–Bubble Interactions Quantified by CP-AFM and Modeling to Understand the Salt Flotation of Battery Black Mass
by Jan Nicklas, Claudia Heilmann, Lisa Ditscherlein and Urs A. Peuker
Minerals 2025, 15(8), 809; https://doi.org/10.3390/min15080809 - 30 Jul 2025
Viewed by 237
Abstract
The salt flotation of graphite in the presence of lithium nickel manganese cobalt oxide (NMC) was assessed by performing colloidal probe atomic force microscopy (CP-AFM) on sessile gas bubbles and conducting batch flotation tests with model lithium-ion-battery black mass. The modeling of film [...] Read more.
The salt flotation of graphite in the presence of lithium nickel manganese cobalt oxide (NMC) was assessed by performing colloidal probe atomic force microscopy (CP-AFM) on sessile gas bubbles and conducting batch flotation tests with model lithium-ion-battery black mass. The modeling of film drainage and detachment during particle–bubble interactions provides insight into the fundamental microprocesses during salt flotation, a special variant of froth flotation. The interfacial properties of particles and gas bubbles were tailored with salt solutions containing sodium chloride and sodium acetate buffer. Graphite particles can attach to gas bubbles under all tested conditions in the range pH 3 to pH 10. The attractive forces for spherical graphite are strongest at high salt concentrations and pH 3. The conditions for the attachment of NMC to gas bubbles were evaluated with simulations using the Stokes–Reynolds–Young–Laplace model for film drainage, under consideration of DLVO forces and a hydrodynamic slip to account for irregularities of the particle surface. CP-AFM measurements in the capillary force regime provide additional parameters for the modeling of salt flotation, such as the force and work of detachment. The contact angles of graphite and NMC particles during retraction and detachment from gas bubbles were obtained from a quasi-equilibrium model using CP-AFM data as input. All CP-AFM experiments and theoretical results suggest that pristine NMC particles do not attach to gas bubbles during flotation, which is confirmed by the low rate of NMC recovery in batch flotation tests. Full article
(This article belongs to the Special Issue Particle–Bubble Interactions in the Flotation Process)
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14 pages, 4484 KiB  
Article
Influence of Particle Size, Defect Density and Salts on the Dissolution and Early Hydration of a Model System (C3A + Quartz)
by Shaoxiong Ye and Pan Feng
Materials 2025, 18(15), 3560; https://doi.org/10.3390/ma18153560 - 29 Jul 2025
Viewed by 253
Abstract
Understanding the hydration behavior of cementitious materials is crucial as it governs the setting, strength development and long-term durability of concrete. This study provides fundamental insights into these processes by investigating the early hydration of tricalcium aluminate (C3A) with quartz as [...] Read more.
Understanding the hydration behavior of cementitious materials is crucial as it governs the setting, strength development and long-term durability of concrete. This study provides fundamental insights into these processes by investigating the early hydration of tricalcium aluminate (C3A) with quartz as a novel model system for multiple clinker phases. Employing a multi-technique approach combining conductivity, calorimetry and microscopy, we systematically examine the concurrent effects of product layer formation, C3A’s particle size and defect density, and salts on dissolution kinetics and early-stage reaction pathways. Results indicate that product layer formation shifted C3A’s rapid dissolution toward diffusion-controlled regimes. Reduced particle size and increased defect density accelerated the dissolution and hydration kinetics. Sulfates and chlorides differentially altered reaction pathways, with preferential sulfate reactivity driving ettringite formation. These mechanistic insights advance fundamental understanding of the hydration behavior of cementitious material. Full article
(This article belongs to the Section Construction and Building Materials)
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12 pages, 1013 KiB  
Article
Investigating the Effect of Zinc Salts on Escherichia coli and Enterococcus faecalis Biofilm Formation
by Sara Deumić, Ahmed El Sayed, Mahmoud Hsino, Andrzej Kulesa, Neira Crnčević, Naida Vladavić, Aja Borić and Monia Avdić
Appl. Sci. 2025, 15(15), 8383; https://doi.org/10.3390/app15158383 - 29 Jul 2025
Viewed by 558
Abstract
Water supply and sewage drainage pipes have a critical role to play in the provision of clean water and sanitation, and pipe material selection influences infrastructure life, water quality, and microbial communities. Zinc-containing compounds are highly valued due to their mechanical properties, anticorrosion [...] Read more.
Water supply and sewage drainage pipes have a critical role to play in the provision of clean water and sanitation, and pipe material selection influences infrastructure life, water quality, and microbial communities. Zinc-containing compounds are highly valued due to their mechanical properties, anticorrosion behavior, and antimicrobial properties. However, the effect of zinc salts, such as zinc sulfate heptahydrate and zinc chloride, on biofilm-forming bacteria, including Escherichia coli and Enterococcus faecalis, is not well established. This study investigates the antibacterial properties of these zinc salts under simulated pipeline conditions using minimum inhibitory concentration assays, biofilm production assays, and antibiotic sensitivity tests. Findings indicate that zinc chloride is more antimicrobial due to its higher solubility and bioavailability of Zn2+ ions. At higher concentrations, zinc salts inhibit the development of a biofilm, whereas sub-inhibitory concentrations enhance the growth of biofilm, suggesting a stress response in bacteria. zinc chloride also enhances antibiotic efficacy against E. coli but induces resistance in E. faecalis. These findings highlight the dual role of zinc salts in preventing biofilm formation and modulating antimicrobial resistance, necessitating further research to optimize material selection for water distribution networks and mitigate biofilm-associated risks in pipeline systems. Full article
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29 pages, 4258 KiB  
Review
Corrosion Performance of Atmospheric Corrosion Resistant Steel Bridges in the Current Climate: A Performance Review
by Nafiseh Ebrahimi, Melina Roshanfar, Mojtaba Momeni and Olga Naboka
Materials 2025, 18(15), 3510; https://doi.org/10.3390/ma18153510 - 26 Jul 2025
Viewed by 507
Abstract
Weathering steel (WS) is widely used in bridge construction due to its high corrosion resistance, durability, and low maintenance requirements. This paper reviews the performance of WS bridges in Canadian climates, focusing on the formation of protective patina, influencing factors, and long-term maintenance [...] Read more.
Weathering steel (WS) is widely used in bridge construction due to its high corrosion resistance, durability, and low maintenance requirements. This paper reviews the performance of WS bridges in Canadian climates, focusing on the formation of protective patina, influencing factors, and long-term maintenance strategies. The protective patina, composed of stable iron oxyhydroxides, develops over time under favorable wet–dry cycles but can be disrupted by environmental aggressors such as chlorides, sulfur dioxide, and prolonged moisture exposure. Key alloying elements like Cu, Cr, Ni, and Nb enhance corrosion resistance, while design considerations—such as drainage optimization and avoidance of crevices—are critical for performance. The study highlights the vulnerability of WS bridges to microenvironments, including de-icing salt exposure, coastal humidity, and debris accumulation. Regular inspections and maintenance, such as debris removal, drainage system upkeep, and targeted cleaning, are essential to mitigate corrosion risks. Climate change exacerbates challenges, with rising temperatures, altered precipitation patterns, and ocean acidification accelerating corrosion in coastal regions. Future research directions include optimizing WS compositions with advanced alloys (e.g., rare earth elements) and integrating climate-resilient design practices. This review highlights the need for a holistic approach combining material science, proactive maintenance, and adaptive design to ensure the longevity of WS bridges in evolving environmental conditions. Full article
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21 pages, 15603 KiB  
Article
Scanning Electron Microscopy of Carbon Nanotube–Epoxy Interfaces: Correlating Morphology to Sulfate Exposure
by Sijan Adhikari, Braiden M. Myers, Bryce L. Tuck, Courtney Dawson, Joey R. Cipriano, Jules F. Ahlert, Menziwokuhle Thwala, Mia A. Griffin, Omar Yadak, Osama A. Alfailakawi, Micah S. Ritz, Seth M. Wright, Jeffery Volz and Shreya Vemuganti
J. Compos. Sci. 2025, 9(8), 392; https://doi.org/10.3390/jcs9080392 - 24 Jul 2025
Viewed by 482
Abstract
Epoxy resins are widely used as protective coatings in civil infrastructure, yet sulfate-rich environments accelerate their deterioration. This study evaluates the effectiveness of multi-walled carbon nanotubes (MWCNTs) in enhancing the sulfate resistance of epoxy resins. Neat and MWCNT-reinforced epoxy specimens (0.25 wt.% and [...] Read more.
Epoxy resins are widely used as protective coatings in civil infrastructure, yet sulfate-rich environments accelerate their deterioration. This study evaluates the effectiveness of multi-walled carbon nanotubes (MWCNTs) in enhancing the sulfate resistance of epoxy resins. Neat and MWCNT-reinforced epoxy specimens (0.25 wt.% and 0.5 wt.%) were fabricated, heat cured at 100 °C and exposed to a solution of sulfuric acid and sodium chloride maintaining a pH of less than 3 for 0, 30, and 60 days. Analytical techniques, including scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS), revealed distinct degradation patterns: the neat epoxy exhibited puncture damage and extensive salt deposition, while the MWCNT-reinforced specimens showed crack propagation mitigated by nanotube bridging. Heat curing introduced micro-voids that exacerbated sulfate ingress. The salt deposition surged to 200 times for the MWCNT-reinforced specimens compared to the neat ones, whereas crack width was higher in the MWCNT reinforced specimen compared to their neat counterparts, given that crack-bridging was observed. These findings highlight the potential of MWCNTs to improve epoxy durability in sulfate-prone environments, though the optimization of curing conditions and dispersion methods is critical. Full article
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9 pages, 4992 KiB  
Communication
Corrosion Behavior of 347H Stainless Steel in NaCl-KCl-MgCl2 Molten Salt: Vapor, Liquid, and Interface Comparison
by Zhiwen Liu, Huigai Li, Yang Wang, Yanjie Peng, Luyan Sun and Jianping Liang
Materials 2025, 18(14), 3412; https://doi.org/10.3390/ma18143412 - 21 Jul 2025
Viewed by 249
Abstract
The suitability of 347H stainless steel (SS347H) for chloride salt environments is critical in selecting materials for next-generation concentrated solar power (CSP) systems. This study investigated the corrosion behavior of SS347H in a ton-scale purification system with continuously flowing chloride salt under three [...] Read more.
The suitability of 347H stainless steel (SS347H) for chloride salt environments is critical in selecting materials for next-generation concentrated solar power (CSP) systems. This study investigated the corrosion behavior of SS347H in a ton-scale purification system with continuously flowing chloride salt under three conditions: exposure to NaCl-KCl-MgCl2 molten salt vapor, immersion in molten salt, and at the molten salt surface interface. Results revealed that corrosion was most severe in the molten salt vapor, where HCl steam facilitated Cl reactions with Fe and Cr in the metal, causing dissolution and forming deep corrosion pits. At the interface, liquid Mg triggered displacement reactions with Fe2+/Cr2+ ions in the salt, depositing Fe and Cr onto the surface, which reduced corrosion intensity. Within the molten salt, Mg’s purification effect minimized impurity-induced corrosion, resulting in the least damage. In all cases, the primary corrosion mechanism involves the dissolution of Fe and Cr, with the formation of minor MgO. These insights provide valuable guidance for applying 347H stainless steel in chloride salt environments. Full article
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14 pages, 1342 KiB  
Article
Mitigating Deicer-Induced Salinity Through Activated Carbon and Salt-Tolerant Grass Integration: A Case of Pennisetum alopecuroides
by Jae-Hyun Park, Hyo-In Lim, Myung-Hun Lee, Yong-Han Yoon and Jin-Hee Ju
Environments 2025, 12(7), 250; https://doi.org/10.3390/environments12070250 - 20 Jul 2025
Viewed by 577
Abstract
The use of chloride-based deicing salts, particularly sodium chloride (NaCl) and calcium chloride (CaCl2), is a common practice in cold regions for maintaining road safety during winter. However, the accumulation of salt residues in adjacent soils poses serious environmental threats, including [...] Read more.
The use of chloride-based deicing salts, particularly sodium chloride (NaCl) and calcium chloride (CaCl2), is a common practice in cold regions for maintaining road safety during winter. However, the accumulation of salt residues in adjacent soils poses serious environmental threats, including reduced pH, increased electrical conductivity (EC), disrupted soil structure, and plant growth inhibition. This study aimed to evaluate the combined effect of activated carbon (AC) and Pennisetum alopecuroides, a salt-tolerant perennial grass, in alleviating salinity stress under deicer-treated soils. A factorial greenhouse experiment was conducted using three fixed factors: (i) presence or absence of Pennisetum alopecuroides, (ii) deicer type (NaCl or CaCl2), and (iii) activated carbon mixing ratio (0, 1, 2, 5, and 10%). Soil pH, EC, and ion concentrations (Na+, Cl, Ca2+) were measured, along with six plant growth indicators. The results showed that increasing AC concentrations significantly increased pH and reduced EC and ion accumulation, with the 5% AC treatment being optimal in both deicer systems. Plant physiological responses were improved in AC-amended soils, especially under CaCl2 treatment, indicating less ion toxicity and better root zone conditions. The interaction effects between AC, deicer type, and plant presence were statistically significant (p < 0.05), supporting a synergistic remediation mechanism involving both adsorption and biological uptake. Despite the limitations of short-term controlled conditions, this study offers a promising phytomanagement strategy using natural adsorbents and salt-tolerant plants for sustainable remediation of salt-affected soils in road-adjacent and urban environments. Full article
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20 pages, 31083 KiB  
Article
Hybrid Mortars Activated with Alternative Steel-Compatible Salts: Impact on Chloride Diffusion and Durability
by Angily Cruz-Hernández, Francisco Velasco, Manuel Torres-Carrasco and Asunción Bautista
Appl. Sci. 2025, 15(14), 8055; https://doi.org/10.3390/app15148055 - 19 Jul 2025
Viewed by 249
Abstract
Eco-friendly mortars have been manufactured with hybrid binders made of blast furnace slag and a reduced amount of clinker. The objective is to explore new formulations suitable for reinforced structures. Previous studies are mainly focused on activation with sulfates, a salt that is [...] Read more.
Eco-friendly mortars have been manufactured with hybrid binders made of blast furnace slag and a reduced amount of clinker. The objective is to explore new formulations suitable for reinforced structures. Previous studies are mainly focused on activation with sulfates, a salt that is corrosive to reinforcing steel. Sodium nitrate and sodium carbonate, easily implementable in construction, have been used as activators in two different concentrations that involve similar Na content. A Type II PC mortar is used as reference. The dimensional stability of the mortars during curing (at 99% RH) and subsequent drying at 40% RH, has been evaluated, as well as their porosity and mechanical properties. Böhme tests revealed that studied hybrid binders have lower wear resistance than PC mortar. Activation with Na2CO3 allows the obtention of mortars with reduced porosity and good compression resistance, but generates microcracking that favors chloride diffusion. Activation with nitrates favors precipitation of AFm phases identified through differential thermal analysis. Nitrates in moderate amounts (4% w/w) allow manufacturing hybrid mortars with good resistance to chloride penetration and reasonably good mechanical properties. Hence, this binder can be a promising option for reinforced structures. Higher amounts of nitrates (8%) for activation give rise to more porous mortars. Full article
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15 pages, 4647 KiB  
Article
Adaptability and Sensitivity of Trichoderma spp. Isolates to Environmental Factors and Fungicides
by Allinny Luzia Alves Cavalcante, Andréia Mitsa Paiva Negreiros, Naama Jéssica de Assis Melo, Fernanda Jéssica Queiroz Santos, Carla Sonale Azevêdo Soares Silva, Pedro Sidarque Lima Pinto, Sabir Khan, Inês Maria Mendes Sales and Rui Sales Júnior
Microorganisms 2025, 13(7), 1689; https://doi.org/10.3390/microorganisms13071689 - 18 Jul 2025
Viewed by 367
Abstract
Biological control employs beneficial microorganisms to suppress phytopathogens and mitigate the incidence of associated plant diseases. This study investigated the in vitro development and survival of Trichoderma spp. isolates derived from commercial formulations under different temperatures, pH levels, and sodium chloride (NaCl) concentrations [...] Read more.
Biological control employs beneficial microorganisms to suppress phytopathogens and mitigate the incidence of associated plant diseases. This study investigated the in vitro development and survival of Trichoderma spp. isolates derived from commercial formulations under different temperatures, pH levels, and sodium chloride (NaCl) concentrations and with synthetic fungicides with distinct modes of action. Three isolates were analyzed: URM-5911 and TRA-0048 (T. asperellum) and TRL-0102 (T. longibrachiatum). The results revealed substantial variability among the isolates, with the optimal mycelial growth temperatures ranging from 24.56 to 29.42 °C. All the isolates exhibited broad tolerance to the tested pH (5–9) and salinity levels (250–1000 mM), with TRL-0102 demonstrating the highest salt resistance. The fungicide treatments negatively affected mycelial growth across all the isolates, with Azoxystrobin + Difenoconazole and Boscalid causing growth reductions of up to 50%. Notably, Boscalid enhanced conidial production more compared to the control (126.0% for URM-5911, 13.7% for TRA-0048, and 148.5% for TRL-0102) and decreased the percentage of inactive conidia to less than 10% in all the isolates. These results provide strategic information for the application of Trichoderma spp. in agricultural systems, supporting the selection of more adapted and suitable isolates for integrated disease management programs. Full article
(This article belongs to the Special Issue Interaction Between Microorganisms and Environment)
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23 pages, 3086 KiB  
Article
Comprehensive Analysis of Soil Physicochemical Properties and Optimization Strategies for “Yantai Fuji 3” Apple Orchards
by Zhantian Zhang, Zhihan Zhang, Zhaobo Fan, Weifeng Leng, Tianjing Yang, Jie Yao, Haining Chen and Baoyou Liu
Agriculture 2025, 15(14), 1520; https://doi.org/10.3390/agriculture15141520 - 14 Jul 2025
Viewed by 338
Abstract
Based on an integrated analysis, this study summarized the current status of soil quality in Yantai apple orchards, developed a multivariate regulation model for key soil physicochemical properties, and proposed optimized fertilization strategies to improve soil quality in the region. The study analyzed [...] Read more.
Based on an integrated analysis, this study summarized the current status of soil quality in Yantai apple orchards, developed a multivariate regulation model for key soil physicochemical properties, and proposed optimized fertilization strategies to improve soil quality in the region. The study analyzed the physicochemical properties of the topsoil (0–30 cm) in 19 representative apple orchards across Yantai, including indicators like pH, organic matter (OM), major nutrient ions, and salinity indicators, using standardized measurements and multivariate statistical methods, including descriptive statistics analysis, frequency distribution analysis, canonical correlation analysis, stepwise regression equation analysis, and regression fit model analysis. The results demonstrated that in apple orchards across the Yantai region, reductions in pH were significantly mitigated under the combined increased OM and exchangeable calcium (Ca). Exchangeable potassium (EK) rose in response to the joint elevation of OM and available nitrogen (AN), and AN was also positively influenced by EK, while OM also exhibited a promotive effect on Olsen phosphorus (OP). Furthermore, Ca increased with higher pH. AN and EK jointly contributed to the increases in electrical conductivity (EC) and chloride ions (Cl), while elevated exchangeable sodium (Na) and soluble salts (SS) were primarily driven by EK. Accordingly, enhancing organic and calcium source fertilizers is recommended to boost OM and Ca levels, reduce acidification, and maintain EC within optimal limits. By primarily reducing potassium’s application, followed by nitrogen and phosphorus source fertilizers, the supply of macronutrients can be optimized, and the accumulation of Na, Cl, and SS can be controlled. Collectively, the combined analysis of soil quality status and the multivariate regulation model clarified the optimized fertilization strategies, thereby establishing a solid theoretical and practical foundation for recognizing the necessity of soil testing and formula fertilization, the urgency of improving soil quality, and the scientific rationale for nutrient input management in Yantai apple orchards. Full article
(This article belongs to the Section Agricultural Soils)
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14 pages, 4419 KiB  
Article
Slurry Aluminizing Mechanisms of Nickel-Based Superalloy and Applicability for the Manufacturing of Platinum-Modified Aluminide Coatings
by Giulia Pedrizzetti, Virgilio Genova, Erica Scrinzi, Rita Bottacchiari, Marco Conti, Laura Paglia and Cecilia Bartuli
Coatings 2025, 15(7), 822; https://doi.org/10.3390/coatings15070822 - 14 Jul 2025
Viewed by 335
Abstract
The slurry aluminizing process is widely employed to enhance the oxidation and corrosion resistance of nickel-based superalloys used in high-temperature environments such as gas turbines and aerospace engines. This study investigates the effects of the concentration of Al vapors in the reactor chamber [...] Read more.
The slurry aluminizing process is widely employed to enhance the oxidation and corrosion resistance of nickel-based superalloys used in high-temperature environments such as gas turbines and aerospace engines. This study investigates the effects of the concentration of Al vapors in the reactor chamber and the initial slurry layer thickness on the microstructure, chemical composition, and phase composition of aluminide coatings. Coatings were manufactured on Ni-based superalloy substrates using CrAl powders as an aluminum source and chloride- and fluoride-based activator salts. The effect of the initial thickness of the slurry layer was studied by varying the amount of deposited slurry in terms of mgslurry/cm2sample (with constant mgslurry/cm3chamber). The microstructure and phase composition of the produced aluminide coatings were evaluated by SEM, EDS, and XRD analysis. Slurry thickness can affect concentration gradients during diffusion, and the best results were obtained with an initial slurry amount of 100 mgslurry/cm2sample. The effect of the Al vapor phase in the reaction chamber was then investigated by varying the mgslurry/cm3chamber ratio while keeping the slurry layer thickness constant at 100 mgslurry/cm2sample. This parameter influences the amount of Al at the substrate surface before the onset of solid-state diffusion, and the best results were obtained for a 6.50 mgslurry/cm3chamber ratio with the formation of 80 µm coatings (excluding the interdiffusion zone) with a β-NiAl phase throughout the thickness. To validate process flexibility, the same parameters were successfully applied to produce platinum-modified aluminides with a bi-phasic ζ-PtAl2 and β-(Ni,Pt)Al microstructure. Full article
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18 pages, 1720 KiB  
Article
In Vitro Preliminary Characterization of Lactiplantibacillus plantarum BG112 for Use as a Starter Culture for Industrial Dry-Fermented Meats
by María Inés Palacio, María Julia Ruiz, María Fernanda Vega and Analía Inés Etcheverría
Fermentation 2025, 11(7), 403; https://doi.org/10.3390/fermentation11070403 - 14 Jul 2025
Viewed by 424
Abstract
The objective of this study was to perform a preliminary in vitro characterization of Lactiplantibacillus plantarum BG112, assessing its safety and technological features for potential application as a culture starter for an industrial fermented dry meat product. In vitro assays assessed its viability, [...] Read more.
The objective of this study was to perform a preliminary in vitro characterization of Lactiplantibacillus plantarum BG112, assessing its safety and technological features for potential application as a culture starter for an industrial fermented dry meat product. In vitro assays assessed its viability, probiotic properties, and safety for use in food formulations. The strain was characterized through morphological and biochemical tests, carbohydrate fermentation profiling, and various in vitro assays based on FAO/WHO criteria for probiotic selection. These included proteolytic activity, auto-aggregation capacity, tolerance to simulated gastric juice and bile salts, antimicrobial activity, and resistance to sodium chloride, nitrite, and low pH. Safety evaluations were also performed by testing antibiotic susceptibility, hemolytic activity, and DNAse production. The results showed that L. plantarum BG112 exhibited strong tolerance to adverse environmental conditions typically found during sausage fermentation and ripening, along with significant inhibitory activity against pathogenic bacteria, such as Escherichia coli O157:H7, Salmonella Typhimurium, and Staphylococcus aureus. The strain also demonstrated no hemolytic or DNAse activity and presented a favorable antibiotic sensitivity profile, meeting key safety requirements for probiotic use. Further studies using meat matrices and in vivo models are needed to validate these findings. This study contributes to the early-stage selection of safe and technologically suitable strains for use in fermented meat products. These findings support the potential application of L. plantarum BG112 as a safe and effective starter culture in the development of high-value, premium fermented meat products, aligned with current consumer demand for health-enhancing and natural foods. Full article
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17 pages, 2428 KiB  
Article
Combining Diluted Seawater and Fertilizer in an Ion-Based Multivariate Approach as an Effective Assay of Salt Tolerance in Brassica juncea Seedlings
by Morgan Tomlin, William Bridges, Qiong Su, Raghupathy Karthikeyan, Byoung Ryong Jeong, Haibo Liu, Gary L. Amy and Jeffrey Adelberg
Horticulturae 2025, 11(7), 820; https://doi.org/10.3390/horticulturae11070820 - 10 Jul 2025
Viewed by 309
Abstract
Non-conventional water sources (saline and brackish water) are viable options for crop cultivation. Current salt-tolerance research largely focuses on Na+ and Cl, while other ions in these waters remain ill-understood. Synthetic seawater was a representative of saline and brackish water [...] Read more.
Non-conventional water sources (saline and brackish water) are viable options for crop cultivation. Current salt-tolerance research largely focuses on Na+ and Cl, while other ions in these waters remain ill-understood. Synthetic seawater was a representative of saline and brackish water in a Design of Experiments (DoE) treatment design used to evaluate the effects of factors [synthetic seawater (0, 15, 30, or 45%, v/v, Instant Ocean®), total inorganic nitrogen (0, 14, or 28 mM; 1 NH4+:8 NO3 ratio), potassium (0, 9, or 21 mM), calcium (0, 2, or 5 mM), silicon (0, 0.03, or 0.09 mM) and zinc (0, 0.05, or 2 mM)] on seedlings for two varieties of Brassica juncea [‘Carolina Broadleaf’ (CB) and ‘Florida Broadleaf’ (FB)] using a hydroponic assay. In 30–45% synthetic seawater, 0.09 mM of silicon or 2 mM of calcium alleviated salt stress. In FB, 0.04–0.06 mM of silicon was optimal for the production of new leaves. The CB variety showed greater production of new leaves with 0.09 mM of silicon and 28 mM of potassium. Potassium and calcium are components of seawater, and a sodium chloride assay would not account for their interactions without a multivariate approach to evaluate salt tolerance. The seedling assay identified factors and established criteria for larger-scale harvest experiments. Full article
(This article belongs to the Topic Plants Nutrients, 2nd Volume)
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15 pages, 887 KiB  
Article
Mapping Ammonium Flux Across Bacterial Porins: A Novel Electrophysiological Assay with Antimicrobial Relevance
by Ishan Ghai
Appl. Sci. 2025, 15(14), 7677; https://doi.org/10.3390/app15147677 - 9 Jul 2025
Viewed by 229
Abstract
This study presents a quantitative electrophysiological method to directly measure the passive transport of ammonium ions through bacterial outer membrane porins. Using a zero-current reversal potential assay in planar lipid bilayers under defined bi-ionic gradients, this study evaluates the permeability of ammonium salts [...] Read more.
This study presents a quantitative electrophysiological method to directly measure the passive transport of ammonium ions through bacterial outer membrane porins. Using a zero-current reversal potential assay in planar lipid bilayers under defined bi-ionic gradients, this study evaluates the permeability of ammonium salts through two general diffusion porins: Omp-Pst2 from Providencia stuartii and OmpF from Escherichia coli. Under matched ionic conditions, Omp-Pst2 exhibited significantly higher ammonium flux—approximately 6000 ions per second per monomer at a 1 µM gradient—compared to ~4000 ions per second for OmpF. Importantly, the identity of the accompanying anion (chloride vs. sulfate) modulated both the ion selectivity and flux rate, highlighting the influence of counterion interactions on porin-mediated transport. These findings underscore how structural differences between porins—such as pore geometry and charge distribution—govern ion permeability. The method applied here provides a robust framework for quantifying nutrient flux at the single-channel level and offers novel insights into how Gram-negative bacteria may adapt their membrane transport mechanisms under nitrogen-limited conditions. This work not only enhances our understanding of outer membrane permeability to small ions like ammonium, but also has implications for antimicrobial strategy development and biotechnological applications in nitrogen assimilation. Full article
(This article belongs to the Special Issue Innovative Digital Health Technologies and Their Applications)
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