Search Results (6,687)

Via a One Health approach, this study concomitantly assessed the susceptibility of humans and dogs to Trypanosoma cruzi infections on three islands and in two mainland seashore areas of southern Brazil. Human serum samples were tested using an enzyme-linked immunosorbent assay (ELISA) to detect anti-T. cruzi antibodies, while dog serum samples were tested using indirect fluorescent antibodies in an immunofluorescence assay (IFA). Seropositive human and dog individuals were also tested using quantitative polymerase chain reaction (qPCR) in corresponding blood samples. Overall, 2/304 (0.6%) human and 1/292 dog samples tested seropositive for T. cruzi by ELISA and IFA, respectively, and these cases were also molecularly positive for T. cruzi by qPCR. Although a relatively low positivity rate was observed herein, these cases were likely autochthonous, and the individuals may have been infected as a consequence of isolated events of disturbance in the natural peridomicile areas nearby. Such a disturbance could come in the form of a fire or deforestation event, which can cause stress and parasitemia in wild reservoirs and, consequently, lead to positive triatomines. In conclusion, T. cruzi monitoring should always be conducted in suspicious areas to ensure a Chagas disease-free status over time. Further studies should also consider entomological and wildlife surveillance to fully capture the transmission and spread of T. cruzi on islands and in seashore mainland areas of Brazil and other endemic countries. Full article

Urban tourism has evolved into a complex adaptive system, where unregulated expansion disrupts the ecological balance and intensifies resource stress. Understanding the dynamic evolution and resilience mechanisms of the tourism ecological health network (TEHN) is essential for supporting sustainable urban tourism as a coupled human–natural system. Using Shanghai as a case study, we applied the "vigor–organization–resilience–services" (VORS) framework to evaluate ecosystem health, which served as a constraint for constructing the TEHN, using the minimum cumulative resistance (MCR) model for the period from 2001 to 2023. A resilience framework integrating structural and functional dimensions was further developed to assess spatiotemporal evolution and guide targeted enhancement strategies. The results indicated that as ecosystem health degraded, particularly in peripheral areas, the urban TEHN in Shanghai shifted from a dispersed to a centralized structure, with limited connectivity in the periphery. The resilience of the TEHN continued to grow, with structural resilience remaining at a high level, while functional resilience still required enhancement. Specifically, the low integration and limited choice between the tourism network and the transportation system hindered tourists from selecting routes with higher ecosystem health indices. Enhancing functional resilience, while sustaining structural resilience, is essential for transforming the TEHN into a multi-centered, multi-level system that promotes efficient connectivity, ecological sustainability, and long-term adaptability. The results contribute to a systems-level understanding of tourism–ecology interactions and support the development of adaptive strategies for balancing network efficiency and environmental integrity. Full article

Background/Objective: Exertional rhabdomyolysis (ER) is primarily driven by mechanical stress on muscles during strenuous or unaccustomed exercise, often exacerbated by environmental factors like heat and dehydration. While the general cellular pathway involving energy depletion and calcium overload is understood in horse ER models, the underlying mechanisms specific to the ER are not universally known within humans. This study aimed to evaluate whether patients with ER exhibited transcriptional signatures that were significantly different from those of healthy individuals. Methods: This study utilized RNA sequencing on skeletal muscle samples from 19 human patients with ER history, collected at a minimum of six months after the most recent ER event, and eight healthy controls to investigate the transcriptomic landscape of ER. To identify any alterations in biological processes between the case and control groups, functional pathway analyses were conducted. Results: Functional pathway enrichment analyses of differentially expressed genes revealed strong suppression of mitochondrial function. This suppression included the “aerobic electron transport chain” and “oxidative phosphorylation” pathways, indicating impaired energy production. Conversely, there was an upregulation of genes associated with adhesion and extracellular matrix-related pathways, indicating active restoration of muscle function in ER cases. Conclusions: The study demonstrated that muscle tissue exhibited signs of suppressed mitochondrial function and increased extracellular matrix development. Both of these facilitate muscle recovery within several months after an ER episode. Full article

We need to educate students and the public about addressing natural resource challenges to maintain civilization moving into a sustainable future. Because US mineral and energy resources are found in its continental crust and sedimentary basins, introductory geology students need to be well-informed about US crust and basins. We think that creating effective videos about these topics is the best way to engage students to want to learn more. In preparation for making these videos, we researched what introductory geology students are taught and what they learn about these topics. Student interviews informed us about learned curriculum, and taught curriculum was analyzed using a novel keyword-counting method applied to textbook indices. We found that geophysics is stressed twice as much as geology, radiometric dating, and sedimentary basins. We expected that students would have learned more about geophysics and less about the other topics; however, this was not the case. Students knew more about geology, and less about geophysics, radiometric dating, and sedimentary basins. To make effective videos on these topics, we need to explain the following threshold concepts: seismic refraction to scaffold student understanding of crustal geophysics, as well as radiometric dating and deep time to understand crustal geology and the economic importance of sedimentary basins. Full article

As a core device in low-voltage distribution networks, the distribution zone transformer (DZT) is influenced by short circuits, overloads, and unbalanced loads, which cause thermal aging, mechanical stress, and eventually deformation of the winding, resulting in parameter deviations from nameplate values and impairing system operation. However, existing identification methods typically require synchronized high- and low-voltage data and are limited to symmetric three-phase conditions, which limits their application in practical distribution systems. To address these challenges, this paper proposes a parameter identification method for DZTs under three-phase unbalanced conditions. Firstly, based on the transformer’s T-equivalent circuit considering the load, the power flow equations are derived without involving the synchronization issue of high-voltage and low-voltage side data, and the sum of the impedances on both sides is treated as an independent parameter. Then, a novel power flow equation under three-phase unbalanced conditions is established, and an adaptive recursive least squares (ARLS) solution method is constructed using the measurement data sequence provided by the smart meter of the intelligent transformer terminal unit (TTU) to achieve online identification of the transformer winding parameters. The effectiveness and robustness of the method are verified through practical case studies. Full article

Varicocele is a common, potentially correctable condition associated with impaired male fertility. Despite being frequently encountered in clinical andrology, its pathophysiological mechanisms, diagnostic criteria, and therapeutic approaches remain areas of active investigation and debate. The authors conducted a comprehensive literature search, using the PubMed database, covering clinical studies, systematic reviews, meta-analyses, and current international guidelines from the past ten years. Emphasis was placed on studies investigating novel diagnostic modalities, therapeutic innovations, and prognostic markers. Emerging evidence supports the multifactorial pathophysiology of varicocele, involving oxidative stress, hypoxia, inflammatory pathways, and potential genetic predisposition. Biomarkers, including microRNAs, antisperm antibodies, and sperm DNA fragmentation, offer diagnostic and prognostic utility, though their routine clinical implementation requires further validation. Advances in imaging, such as shear wave elastography, may improve diagnostic accuracy. While microsurgical subinguinal varicocelectomy remains the gold standard, technological refinements and non-surgical alternatives are being explored. Indications for treatment have expanded to include selected cases of non-obstructive azoospermia, hypogonadism, and optimization for assisted reproduction, though high-level evidence is limited. Full article

Silvopastoral systems, though ecologically beneficial, remain underrepresented in the European Union’s Common Agricultural Policy and are seldom studied in Mediterranean contexts. The current study assesses both the environmental and economic aspects of five typical silvopastoral systems in central Greece, encompassing cattle, sheep, and goat farming. A Life Cycle Assessment approach was implemented to quantify greenhouse gas emissions using economic allocation, distributing impacts between milk and meat outputs. Enteric fermentation was the major emission source, accounting for up to 65.14% of total emissions in beef-based systems, while feeding and soil emissions were more prominent in mixed and small ruminant systems. Total farm-level emissions ranged from 60,609 to 273,579 kg CO₂eq per year. Economically, only beef-integrated systems achieved an average annual profitability above EUR 20,000 per farm, based on financial data averaged over the last five years (2020–2024) from selected case studies in central Greece, while the remaining systems fell below the national poverty threshold for an average household, underscoring concerns about their economic viability. The findings underline the dual challenges of economic viability and policy neglect, stressing the need for targeted support if these multifunctional systems are to add value to EU climate goals and rural sustainability. Full article

The placement of hydraulic elements in existing pool-type fishways to make them more suitable for Cyprinid fish is an issue of increasing interest in fishway research. Hydrodynamic characteristics and fish behavior at the representative pool of the fishway with bottom orifices and notches were assessed at the Dagdelen hydropower plant in the Ceyhan River Basin, Türkiye. Three-dimensional velocity measurements were taken in the pool of the fishway using an Acoustic Doppler velocimeter. The measurements were taken with and without a brush block at two different vertical distances from the bottom, which were below and above the level of bristles tips. A computational fluid dynamics (CFD) analysis was conducted for the studied fishway. The numerical model utilized Large Eddy Simulation (LES) combined with the Darcy–Forchheimer law, wherein brush blocks were represented as homogenous porous media. Our results revealed that the relative submergence of bristles in the brush block plays a very important role in velocity and Reynolds shear stress (RSS) distributions. After the placement of the submerged brush block, flow velocity and the lateral RSS component were reduced, and a resting area was created behind the brush block below the bristles’ tips. Fish movements in the pool were recorded by underwater cameras under real-time operation conditions. The heatmap analysis, which is a 2-dimensional fish spatial presence visualization technique for a specific time period, showed that Capoeta damascina avoided the areas with high turbulent fluctuations during the tests, and 61.5% of the fish presence intensity was found to be in the low Reynolds shear regions in the pool. This provides a clear case for the real-world ecological benefits of retrofitting existing pool-weir fishways with such flexible hydraulic elements. Full article

Coarse-grained soils serve as essential fill materials in earth–rock dam engineering, where their mechanical properties critically influence dam deformation and stability, directly impacting project safety. Artificial intelligence (AI) techniques are emerging as powerful tools for predicting the mechanical properties of coarse-grained soils. However, AI-based prediction models for these properties face persistent challenges, particularly in parameter tuning—a process requiring substantial computational resources, extensive time, and specialized expertise. To address these limitations, this study proposes a novel prediction model that integrates Adaptive Simulated Annealing (ASA) with an improved Particle Swarm Optimization (PSO) algorithm to optimize the Elman Neural Network (ENN). The methodology encompasses three key aspects: First, the standard PSO algorithm is enhanced by dynamically adjusting its inertial weight and learning factors. The ASA algorithm is then employed to optimize the Adaptive PSO (APSO), effectively mitigating premature convergence and local optima entrapment during training, thereby ensuring convergence to the global optimum. Second, the refined PSO algorithm optimizes the ENN, overcoming its inherent limitations of slow convergence and susceptibility to local minima. Finally, validation through real-world engineering case studies demonstrates that the ASA-PSO-optimized ENN model achieves high accuracy in predicting the mechanical properties of coarse-grained soils. This model provides reliable constitutive parameters for stress–strain analysis in earth–rock dam engineering applications. Full article

Background/Objectives: Dental practitioners widely use dental implants to treat traumatic cases. Titanium implants are currently the most popular choice among dental practitioners and surgeons. The discovery of newer polymeric materials is also influencing the interest of dental professionals in alternative options. A comparative study between existing titanium implants and newer polymeric materials can enhance professionals’ ability to select the most suitable implant for a patient’s treatment. This study aimed to investigate material property advantages of high-performance thermoplastic biopolymers such as PEEK and PEKK, as compared to the time-tested titanium implants, and to find the most suitable and economically fit implant material. Methods: Three distinct implant material properties were assigned—PEEK, PEKK, and commercially pure titanium (CP Ti-55)—to dental implants measuring 5.5 mm by 9 mm, along with two distinct titanium (TI6AL4V) abutments. Twelve three-dimensional (3D) models of bone blocks, representing the mandibular right molar area with Osseo-integrated implants were created. The implant, abutment, and screw were assumed to be linear; elastic, isotropic, and orthotropic properties were attributed to the cancellous and cortical bone. Twelve model sets underwent a three-dimensional finite element analysis to evaluate von Mises stress and total deformation under 250 N vertical and oblique (30 degree) loads on the top surface of each abutment. Results: The study revealed that the time-tested titanium implant outperforms PEEK and PEKK in terms of marginal bone preservation, while PEEK outperforms PEKK. Conclusions: This study will assist dental practitioners in selecting implants from a variety of available materials and will aid researchers in their future research. Full article

The present paper proposes a three-dimensional (3D) spherical shell model for the magneto-electro-elastic (MEE) free vibration analysis of simply supported multilayered smart shells. A mixed curvilinear orthogonal reference system is used to write the unified 3D governing equations for cylinders, cylindrical panels and spherical shells. The closed-form solution of the problem is performed considering Navier harmonic forms in the in-plane directions and the exponential matrix method in the thickness direction. A layerwise approach is possible, considering the interlaminar continuity conditions for displacements, electric and magnetic potentials, transverse shear/normal stresses, transverse normal magnetic induction and transverse normal electric displacement. Some preliminary cases are proposed to validate the present 3D MEE free vibration model for several curvatures, materials, thickness values and vibration modes. Then, new benchmarks are proposed in order to discuss possible effects in multilayered MEE curved smart structures. In the new benchmarks, first, three circular frequencies for several half-wave number couples and for different thickness ratios are proposed. Thickness vibration modes are shown in terms of displacements, stresses, electric displacement and magnetic induction along the thickness direction. These new benchmarks are useful to understand the free vibration behavior of MEE curved smart structures, and they can be used as reference for researchers interested in the development of of 2D/3D MEE models. Full article

With the rise of carbon capture and storage, liquefied carbon dioxide (LCO₂) has emerged as a promising medium for large-scale marine transport. This study evaluates the structural integrity of an IMO Type C cargo tank for a medium-range LCO₂ carrier under four conditions: ultimate limit state, accidental limit state, hydrostatic pressure test, and fatigue limit state, based on IGC Code and classification rules. Seventeen load cases were analyzed using finite element methods with multi-step loading to ensure stability. The highest stress occurred at the pump dome–shell junction due to geometric discontinuities, but all stress and buckling criteria were satisfied. The fatigue damage from wave-induced loads was negligible, with low-cycle fatigue from loading/unloading operations governing the fatigue life, which exceeded 31,000 years. The findings confirm the tank’s structural robustness and its suitability for safe, efficient medium-pressure LCO₂ transport. Full article

Underground blasting vibrations are a critical factor influencing the stability of mine slopes. However, existing studies have yet to establish a quantitative relationship or clarify the underlying mechanisms linking blasting-induced vibrations and slope deformation. Taking the Shilu Iron Mine as a case study, this research develops a dynamic mechanical response model of slope stability that accounts for blasting loads. By integrating slope radar remote sensing data and applying the Pearson correlation coefficient, this study quantitatively evaluates—for the first time—the correlation between underground blasting activity and slope surface deformation. The results reveal that blasting vibrations are characterized by typical short-duration, high-amplitude pulse patterns, with horizontal shear stress identified as the primary trigger for slope shear failure. Both elevation and lithological conditions significantly influence the intensity of vibration responses: high-elevation areas and structurally loose rock masses exhibit greater dynamic sensitivity. A pronounced lag effect in slope deformation was observed following blasting, with cumulative displacements increasing by 10.13% and 34.06% at one and six hours post-blasting, respectively, showing a progressive intensification over time. Mechanistically, the impact of blasting on slope stability operates through three interrelated processes: abrupt perturbations in the stress environment, stress redistribution due to rock mass deformation, and the long-term accumulation of fatigue-induced damage. This integrated approach provides new insights into slope behavior under blasting disturbances and offers valuable guidance for slope stability assessment and hazard mitigation. Full article

Exposure to xenobiotics causes pathophysiological changes in fish, including oxidative stress and neurotoxicity. Here, we describe the biochemical mechanisms underlying oxidative stress (i.e., redox imbalance) and the biochemical markers commonly used to assess its level. Neurotoxicity biomarkers used in fish include behavioral, histological, molecular, neurotransmitter-related, and enzymatic parameters, among which acetylcholinesterase (AChE) activity is the most commonly measured. We therefore also review the changes in AChE activity in fish exposed to common xenobiotics. In most cases, AChE activity decreased in a concentration- and time-dependent manner, although some studies reported no change or even an increase. We emphasize the relevance of all the parameters discussed in the context of fish toxicology studies. Full article

According to the Formula 1 commitment to produce net zero carbon emissions by 2030, the present paper examines the environmental impact of Formula 1 logistics by means of a case study carried out from the point of view of an Italian company, with reference to the European Grand Prix. Logistics accounts for approximately 49% of the sport’s total emissions and accordingly, to reduce its carbon footprint, addressing the logistics activity is vital. Two scenarios are compared in detail: AS-IS, involving only road transport of assets, and TO-BE, in which a combined rail–road approach (i.e., intermodal freight) is implemented. While the AS-IS scenario is more cost-effective, it has a significant environmental impact in terms of CO₂ emissions; in contrast, though more complex and costly, TO-BE offers major advantages for environmental sustainability, including reduced emissions (approximately half compared to AS-IS) and improved efficiency through intermodal transport units. This study stresses that a combined transport system, facilitated by the European rail infrastructure, is a more sustainable option for Formula 1 logistics. However, achieving full carbon neutrality still represents a challenge that will require further innovations and collaboration among the stakeholders of this world. Full article