Search Results (1,666)

The food industry consumes large amounts of water across various processes, and generates wastewater characterized by parameters like biochemical oxygen demand, chemical oxygen demand, pH, suspended solids, and nutrients. To meet environmental standards and enable reuse or valorization, treatment methods such as physicochemical, biological, and membrane-based processes are applied. This review focuses on the valorization of food industry wastewater in the biotechnological production of high-value products, with an emphasis on starch-rich wastewater, wineries and confectionery industry wastewater, and with a focus on new technologies for reduces environmental burden but also supports circular economy principles. Starch-rich wastewaters, particularly those generated by the potato processing industry, offer considerable potential for biotechnological valorization due to their high content of soluble starch, proteins, organic acids, minerals, and lipids. These effluents can be efficiently converted by various fungi (e.g., Aspergillus, Trichoderma) and yeasts (e.g., Rhodotorula, Candida) into value-added products such as lipids for biodiesel, organic acids, microbial proteins, carotenoids, and biofungicides. Similarly, winery wastewaters, characterized by elevated concentrations of sugars and polyphenols, have been successfully utilized as medium for microbial cultivation and product synthesis. Microorganisms belonging to the genera Aspergillus, Trichoderma, Chlorella, Klebsiella, and Xanthomonas have demonstrated the ability to transform these effluents into biofuels, microbial biomass, biopolymers, and proteins, contributing to sustainable bioprocess development. Additionally, wastewater from the confectionery industry, rich in sugars, proteins, and lipids, serves as a favorable fermentation medium for the production of xanthan gum, bioethanol, biopesticides, and bioplastics (e.g., PHA and PHB). Microorganisms of the genera Xanthomonas, Bacillus, Zymomonas, and Cupriavidus are commonly employed in these processes. Although there are still certain regulatory issues, research gaps, and the need for more detailed economic analysis and kinetics of such production, we can conclude that this type of biotechnological production on waste streams has great potential, contributing to environmental sustainability and advancing the principles of the circular economy. Full article

Accurate and efficient estimation of microalgae cell concentration is critical for applications in hydrochemical monitoring, biofuel production, pharmaceuticals, and ecological studies. Traditional methods, such as manual counting with a hemocytometer, are time-consuming and prone to human error, while automated systems are often costly and require extensive training data. This paper presents a low-cost, automated approach for estimating cell concentration in Chlorella vulgaris suspensions using classical computer vision techniques. The proposed method eliminates the need for deep learning by leveraging the Hough circle transform to detect and count cells in microscope images, combined with a conversion factor to translate pixel measurements into metric units for direct concentration calculation (cells/mL). Validation against manual hemocytometer counts demonstrated strong agreement, with a Pearson correlation coefficient of 0.96 and a mean percentage difference of 17.96%. The system achieves rapid processing (under 30 s per image) and offers interpretability, allowing specialists to verify results visually. Key advantages include affordability, minimal hardware requirements, and adaptability to other microbiological applications. Limitations, such as sensitivity to cell clumping and impurities, are discussed. This work provides a practical, accessible solution for laboratories lacking expensive automated equipment, bridging the gap between manual methods and high-end technologies. Full article

Ethanol upgrading via catalytic C–C coupling, commonly known as the Guerbet reaction, offers a sustainable route to produce 1-butanol, a high-performance biofuel. To address gaps in the mechanistic understanding of the catalytic reaction, we investigated the process involving a fixed-bed reactor, operated at 275–325 °C, 21 bar, and weight hourly space velocities of 0.25–2.5 g_EtOH/(g_cat·h), using helium as a carrier gas, with a 5:1 He/EtOH molar ratio. The catalyst was a MgO–Al₂O₃ mixed oxide (Mg/Al = 2:1), derived from a hydrotalcite precursor. A detailed kinetic model was developed, encompassing 15 species and 27 reversible steps (10 sorption and 17 reaction steps), within a 1+1D sorption–reaction–transport framework. Four C₄-forming pathways were included: aldol condensation to form crotonaldehyde, semi-direct coupling to form butyraldehyde and crotyl alcohol, and direct coupling to form 1-butanol. To avoid overfitting, Arrhenius parameters were grouped by reaction type, resulting in sixty rate parameters and one active site-specific density parameter. The optimized model achieved high accuracy, with an average prediction error of 1.44 times the experimental standard deviation. The mechanistic analysis revealed aldol condensation as the dominant pathway below 335 °C, with semi-direct coupling to crotyl alcohol prevailing above 340 °C. The resulting model provides a robust framework for understanding and predicting complex reaction networks in ethanol upgrading systems. Full article

International efforts are underway to implement carbon neutrality policies in rapidly changing climate conditions. This situation has strongly demanded the discovery of novel carbon sinks. The Salix genus has attracted attention as a promising carbon sink owing to its rapid growth and efficient use as a biofuel in short-rotation cultivation. The present study aims to derive an allometric equation and conduct stem analysis as fundamental tools for estimating net primary productivity (NPP) in Salix pierotii Miq. stand, which is increasingly acknowledged as an important emerging carbon sink. The allometric equations derived showed a high explanatory rate and fitness (R² ranged from 0.74 to 0.99). The allometric equations between DBH and stem volume and biomass derived in the process of stem analysis also showed a high explanatory rate and fitness (R² ranged from 0.87 to 0.94). The NPPs calculated based on the allometric equation derived and stem analysis were 11.87 tonC∙ha⁻¹∙yr⁻¹ and 15.70 tonC∙ha⁻¹∙yr⁻¹, respectively. These results show that the S. pierotii community, recognized as the representative riparian vegetation, could play an important role as a carbon sink. In this context, an assessment of the carbon absorption capacity of riparian vegetation such as willow communities could contribute significantly to achieving carbon neutrality goals. Full article

The increasing demand for sustainable energy has spurred the exploration of advanced technologies for biodiesel production. This paper investigates the use of Dielectric Barrier Discharge (DBD)-generated low-temperature plasmas to enhance the conversion of fatty acid methyl esters (FAMEs) into hydrogenated fatty acid methyl esters (H-FAMEs) and other high-value hydrocarbons. A key mechanistic advance is achieved via in situ distillation: at the reactor temperature, unsaturated C18 and C20 FAMEs remain liquid due to their low melting points, while the corresponding saturated C18:0 and C20:0 FAMEs (with melting points of approximately 37–39 °C and 46–47 °C, respectively) solidify and deposit on a glass substrate. This phase separation continuously exposes fresh unsaturated FAME to the plasma, driving further hydrogenation and thereby delivering high overall conversion efficiency. The non-thermal, energy-efficient nature of DBD plasmas offers a promising alternative to conventional high-pressure, high-temperature methods; here, we evaluate the process efficiency, product selectivity, and scalability of this room-temperature, atmospheric-pressure approach and discuss its potential for sustainable fuel-reforming applications. Full article

In this study, we tested a flexible polyurethane (PU) foam, synthesized from bio-based components, for the removal of petroleum-derived fuels from water samples. The PU was synthesized via the prepolymer method through the reaction of PEG 400 with L-lysine ethyl ester diisocyanate (L-LDI), followed by chain extension with 2,5-bis(hydroxymethyl)furan (BHMF), a renewable platform molecule derived from carbohydrates. Freshwater and seawater samples were artificially contaminated with commercial diesel, gasoline, and kerosene. Batch adsorption experiments revealed that the total sorption capacity (S, g/g) of the PU was slightly higher for diesel in both water types, with values of 67 g/g in freshwater and 70 g/g in seawater. Sorption kinetic analysis indicated that the process follows a pseudo-second-order kinetic model, suggesting strong chemical interactions. Equilibrium data were fitted using Langmuir and Freundlich isotherm models, with the best fit achieved by the Langmuir model, supporting a monolayer adsorption mechanism on homogeneous surfaces. The PU foam can be regenerated up to 50 times by centrifugation, maintaining excellent performance. This study demonstrates a promising application of this sustainable and bio-based polyurethane foam for environmental remediation. Full article

The generation of fine coal particles during mining and processing presents significant environmental and logistical challenges, particularly in coal-dependent, developing countries like South Africa (SA). This review critically evaluates the technical viability of fine coal briquetting as a sustainable waste-to-energy solution within a SA context, while drawing from global best practices and comparative benchmarks. It examines abundant feedstocks that can be used for valorization strategies, including fine coal and agricultural biomass residues. Furthermore, binder types, manufacturing parameters, and quality optimization strategies that influence briquette performance are assessed. The co-densification of fine coal with biomass offers a means to enhance combustion efficiency, reduce dust emissions, and convert low-value waste into a high-calorific, manageable fuel. Attention is also given to briquette testing standards (i.e., South African Bureau of Standards, ASTM International, and International Organization of Standardization) and end-use applications across domestic, industrial, and off-grid settings. Moreover, the review explores socio-economic implications, including rural job creation, energy poverty alleviation, and the potential role of briquetting in SA’s ‘Just Energy Transition’ (JET). This paper uniquely integrates technical analysis with policy relevance, rural energy needs, and practical challenges specific to South Africa, while offering a structured framework for bio-coal briquetting adoption in developing countries. While technical and economic barriers remain, such as binder costs and feedstock variability, the integration of briquetting into circular economy frameworks represents a promising path toward cleaner, decentralized energy and coal waste valorization. Full article

The robust structure of algal cell walls presents a major barrier in the recovery of fermentable sugars and intracellular lipids for biofuel production. This study investigates the effectiveness of ultraviolet (UV) radiation and UV-assisted hydrogen peroxide (UV/H₂O₂) pretreatment on a local mixed marine algal culture to enhance biofuel production through cell wall disruption. Local mixed cultures of marine microalgae (LMCMA) were pretreated with UV for various exposure times (5–30 min) and with UV/H₂O₂ using H₂O₂ concentrations ranging from 0.88 to 3.53 mM. The impact of pretreatment was evaluated based on morphological changes (SEM and TEM), elemental composition (C, H, N), sugar release, and downstream fermentation yields of ethanol, methanol, 1-propanol, 1-butanol, and 1-pentanol using Saccharomyces cerevisiae. UV pretreatment at 20–30 min yielded the highest carbohydrate release (up to 0.025 g/gDCW), while UV/H₂O₂ at 1.76 mM achieved maximum sugar liberation (0.0411 g/gDCW). Fermentation performance was enhanced under optimized conditions, with peak ethanol yields of 0.3668 g ethanol/g carbohydrates (UV, 30 min, 48 h) and 0.251 g ethanol/g (UV/H₂O₂, 0.88 mM, 24 h). This study also demonstrated selective production of higher alcohols under varying fermentation temperatures (30–37 °C). These findings highlight the potential of combining oxidative pretreatment and process optimization to enhance biofuel recovery from environmentally relevant algal biomass. Full article

This study evaluated the viability of using the solid residues (bagasse) of the mezcal industry produced with Agave durangensis as a substrate for biogas production, using two chemical pretreatments, acid (HCl) and alkaline (KOH + Ca(OH)₂), to enhance its biodegradability and improve the anaerobic digestion (AD) process. The chemical composition of bagasse was analyzed before and after the chemical pretreatments and then AD experiments were conducted in anaerobic sequential batch reactors (A-SBR) to analyze the effect of pretreatments on biogas production performance. The results showed that acid pretreatment increased cellulose content to 0.606 g, which represented an increase of 34%, and significantly reduced hemicellulose. In contrast, alkaline pretreatment did not show significant changes in cellulose composition, although it caused a swelling of the Agave durangensis mezcal bagasse (Ad-MB) fibers. In terms of biogas production, Ad-MB pretreated with acid (Ad-MB-acid) increased cumulative production by 76% compared to the Agave durangensis mezcal bagasse that was not pretreated (Ad-MB-not pretreated) and by 135% compared to Agave durangensis mezcal bagasse pretreated with an alkaline solution (Ad-MB-alkaline). These results confirmed that Agave durangensis solid waste from the mezcal industry that receives acidic chemical pretreatment has the potential to generate biogas as a sustainable biofuel that can be used to reduce the ecological footprint of this industry. Full article

This work investigated the effect of experimental conditions of dry and wet torrefaction on the properties of olive leaves and olive pomace. Torrefaction improved the fuel properties of olive waste. According to Van Krevelen parameters (O/C and H/C ratios), torrefied biomass, tested as solid biofuel, achieved a similar quality threshold to lignite. For example, dry torrefaction conducted at 230 °C for 80 min reduced the O/C and H/C ratios of olive leaves from 0.51 and 1.51 for raw biomass to 0.25 and 1.17 for torrefied biomass, respectively. Under the same conditions, the O/C and H/C ratios of olive pomace were also reduced from 0.34 and 1.60 to 0.27 and 1.36, respectively. Calorific values of raw olive leaves and olive pomace amounted to 18.0 and 23.2 MJ/kg, respectively. Following dry torrefaction and biomass conversion into biochar, calorific values of olive leaves and olive pomace increased by 24% and 14% up to 22.2 and 26.3 MJ/kg through dry torrefaction, compared with 17% and 23% increments up to 21.1 and 28.5 MJ/kg through wet torrefaction, respectively. Interestingly, biomass processing through wet torrefaction performed in a fluidized bed powered by superheated steam could be completed 8- to 12-fold more rapidly than dry torrefaction. SEM analysis indicated a breakdown of the surface structure of olive waste following the torrefaction process. According to the Brunauer–Emmett–Teller (BET) method, total pore surface areas of biochar obtained from wet torrefaction of olive pomace and olive leaves amounted to 3.6 m²/g and 0.8 m²/g, with total pore volumes amounting to 0.0225 cm³/g and 0.0103 cm³/g, respectively. Maximal contents of 5-hydroxymethylfurfural and furfural in liquid by-products from dry torrefaction amounted to 1930 and 1880 mg/1 kg, respectively. Alternately, in liquid by-products from wet torrefaction, concentrations of these high-value compounds remained very low. Full article

The dependence of conventional epoxy resins on fossil fuels and the environmental and health hazards associated with bisphenol A (BPA) demand the creation of sustainable alternatives. Because lignin is a natural resource and has an aromatic ring skeleton structure, it could be used as an alternative to fossil fuels. This study effectively resolved this challenge by utilizing a sustainable one-step epoxidation process to transform lignin into a bio-based epoxy resin. The results verified the successful synthesis of epoxidized bamboo lignin through systematic characterization employing Fourier transform infrared spectroscopy, hydrogen spectroscopy/two-dimensional heteronuclear single-quantum coherent nuclear magnetic resonance, quantitative phosphorus spectroscopy, and gel permeation chromatography. Lignin-based epoxy resins had an epoxy equivalent value of 350–400 g/mol and a weight-average molecular weight of 4853 g/mol. Studies on the curing kinetics revealed that polyetheramine (PEA-230) demonstrated the lowest apparent activation energy (46.2 kJ/mol), signifying its enhanced curing efficiency and potential for energy conservation. Mechanical testing indicated that the PEA-230 cured network demonstrated the maximum tensile strength (>25 MPa), whereas high-molecular-weight polyetheramine (PEA-2000) imparted enhanced elongation to the material. Lignin-based epoxy resins demonstrated superior heat stability. This study demonstrates the conversion of bamboo lignin into bio-based epoxy resins using a simple, environmentally friendly synthesis process, demonstrating the potential to reduce fossil resource use, efficiently use waste, develop sustainable thermosetting materials, and promote a circular bioeconomy. Full article

Significant wastes such as bagasse, molasses, and vinasses are produced during sugarcane processing. Due to their high sugar content, these wastes are commonly used as low-cost substrates for biofuel production. However, these substrates are also suitable for the microbial synthesis of high-value biochemicals like biosurfactants. Sporisorium scitamineum, a smut fungus capable of growing on sugarcane residues and producing mannosylerythritol lipids (MELs) and cellobiose lipids (CBLs), was identified as a promising candidate for valorizing sugarcane wastes. This study investigated MEL and CBL co-production from pure sugars and sugarcane molasses using an S. scitamineum strain isolated from sugarcane residues originating from KwaZulu-Natal, South Africa. Among the sugars tested, sucrose supported the highest glycolipid production, yielding 0.24 g/L MELs and 2.73 g/L CBLs. Lower titers were achieved with fructose, and no production occurred with glucose. Sugarcane molasses also proved to be an effective substrate, yielding 1.46 g/L CBLs—the highest reported titer from an industrial waste to date. However, all titers remained far below those of other glycolipids, which consistently exceed 50 g/L. Future efforts should focus on enhancing CBL production through process optimization or genetic engineering. Full article

The increasing global demand for energy and the urgent need to mitigate climate change have driven the search for sustainable alternatives to fossil fuels, with biofuels emerging as a promising solution. However, the low yields and inefficiencies in biofuel production processes necessitate advanced strategies to enhance their commercial viability. Metabolic engineering has become a pivotal tool in optimizing microbial pathways to improve biofuel production, addressing these challenges through innovative genetic and synthetic biology approaches. This review highlights the role of metabolic engineering in enhancing biofuel production by focusing on microbial engineering for lignocellulosic biomass utilization, strategies to overcome inhibitor effects, and pathway optimization for biofuels like n-butanol and iso-butanol. It also explores the production of advanced biofuels from fatty acid and isoprenoid pathways, emphasizing the use of model organisms such as Escherichia coli and Saccharomyces cerevisiae. Key insights include the application of CRISPR/Cas9 and multiplex automated genome engineering for precise genetic modifications, as well as metabolic flux analysis to optimize pathway efficiency. Additionally, the review discusses synthetic biology methodologies to rewire metabolic networks and improve biofuel yields, providing a comprehensive overview of current advancements and their implications for industrial-scale production. Full article

This paper aims to fill a gap in relation to herbaceous biomass, which is the second most important source of biomass used for the production of solid biofuels in the form of pellets. Our research focuses on the final stage of compacted fuel production, specifically on the pressure densification of biomass into pellets. This review was conducted using bibliometric techniques. This analysis included searching indexed publications in the Web of Science—Core Collection and Scopus databases. The time range of the searched data was 1991–2024. First, bibliometric data were collected and quantitative analyses were performed. The next stage consisted of analyzing keywords from the authors’ publications using the VOSviewer program. The generated term maps allowed for the analysis of the frequency of keywords in different years and the most frequently cited ones, which enabled the identification of current research topics and the evolution of trends. Based on the collected data, applications, process parameters and raw materials used in the biomass densification process were determined. Following the bibliometric analysis, detailed analyses were carried out on the factors influencing the pressure compaction process. Full article

Addressing the urgent need to decarbonise aviation and valorise agricultural waste, this paper investigates the production of Sustainable Aviation Fuel (SAF) from corn stover. A preliminary evaluation based on a literature review indicates that among various conversion technologies, fast pyrolysis (FP) emerged as the most promising option, offering the highest fuel yield (22.5%) among various pathways, a competitive potential minimum fuel selling price (MFSP) of 1.78 USD/L, and significant greenhouse gas savings of up to 76%. Leveraging Aspen Plus simulation, SAF production via FP was rigorously designed and optimised, focusing on the heat integration strategy within the process to minimise utility consumption and ultimately the total cost. Consequently, the produced fuel exceeded the American Society for Testing and Materials (ASTM) limit for the final boiling point, rendering it unsuitable as a standalone jet fuel. Nevertheless, it achieves regulatory compliance when blended at a rate of up to 10% with conventional jet fuel, marking a practical route for early adoption. Energy optimisation through pinch analysis integrated four hot–cold stream pairs, eliminating external heating, reducing cooling needs by 55%, and improving sustainability and efficiency. Economic analysis revealed that while heat integration slashed utility costs by 84%, the MFSP only decreased slightly from 2.35 USD/L to 2.29 USD/L due to unchanging material costs. Sensitivity analysis confirmed that hydrogen, catalyst, and feedstock pricing are the most influential variables, suggesting targeted reductions could push the MFSP below 2 USD/L. In summary, this work underscores the technical and economic viability of corn stover-derived SAF, providing a promising pathway for sustainable aviation and waste valorisation. While current limitations restrict fuel quality during full substitution, the results affirm the feasibility of SAF blending and present a scalable, low-carbon pathway for future development. Full article