Search Results (5,889)

Background/Objectives: Topical treatment of cutaneous tuberculosis (CTB) requires reliable models to evaluate dermal drug release and diffusion, particularly for fixed-dose combinations (FDCs) with contrasting physicochemical properties. Human skin remains the reference standard but poses ethical, logistical, and reproducibility challenges. This study investigated the suitability of Strat-M^® synthetic membranes as an alternative to human skin for assessing the simultaneous release and diffusion of clofazimine (CFZ) and pyrazinamide (PZA) from a topical FDC, and aimed to develop an optimized dermal emulsion using a Quality-by-Design (QbD)-informed formulation development tool. Methods: Self-emulsifying dermal emulsions containing CFZ and PZA were developed following QbD principles. Preformulation studies included drug solubility screening, oil phase selection, and pseudoternary phase diagram construction to identify stable emulsion regions. Formulations were characterized for droplet size, polydispersity index, zeta potential, viscosity, self-emulsification efficiency, and thermodynamic stability. Eight stable emulsions were identified, of which four were selected for in vitro drug release studies. The peppermint oil-based emulsion (PPO415) was further evaluated in comparative diffusion studies using Strat-M^® membranes and ex vivo human skin (Caucasian and African). Results: PPO415 demonstrated favorable physicochemical properties, including high CFZ solubility, uniform droplet distribution, and suitability for dermal application. Comparative diffusion studies showed that Strat-M^® underestimated the partitioning of lipophilic CFZ while overestimating the diffusion of hydrophilic PZA relative to human skin. These differences were attributed to compositional and structural disparities between synthetic membranes and biological skin. Conclusions: Strat-M^® membranes show potential as a reproducible and ethical in vitro screening tool during early-stage formulation development for topical FDCs. However, ex vivo human skin remains essential for accurately predicting dermal drug distribution and therapeutic performance. Full article

Food fermentation is an ancient bioprocess characterized by complex biochemical transformations driven primarily by microbial communities. Across the diverse regions of China, various ethnic groups have developed a rich array of traditional fermented foods through long-term practical experience. These foods are integral to local culinary heritage and provide valuable systems for studying microbial ecology and function. From the perspective of microbial interactions, this review summarizes key concepts and major interaction types—including mutualism, commensalism, and competition—and describes how bacteria, yeasts, and molds interact via metabolic division of labor to drive substrate conversion, flavor formation, preservation, and biosynthesis of functional compounds. Focusing on four representative ethnic fermented foods—Dong fermented fish, Mongoslian milk curd, Miao sour soup, and Manchurian kombucha—we analyze how microbial interactions contribute to product quality, safety, and sensory attributes. Given current challenges in industrializing traditional fermented foods, such as poor standardization and variable quality, we propose future research directions centered on modern microbiome tools, designed microbial consortia, and process optimization. This work aims to provide a scientific foundation and practical strategies for modernization and quality improvement of traditional fermented foods. Full article

Targeting the thinly bedded and strongly heterogeneous tight sandstone gas reservoirs of the Shaximiao Formation in the Yingshan area of the Sichuan Basin, this study establishes an integrated workflow that combines high-fidelity 3D seismic processing with quantitative interpretation to address key challenges such as insufficient resolution of conventional seismic data under complex near-surface conditions and difficulty in depicting sand-body geometries. On the processing side, a 2D-3D integrated amplitude-preserving high-resolution strategy is applied. In contrast to conventional workflows that treat 2D and 3D datasets independently and often sacrifice true-amplitude characteristics during static correction and noise suppression, the proposed approach unifies first-break picking and static-correction parameters across 2D and 3D data while preserving relative amplitude fidelity. Techniques such as true-surface velocity modeling, coherent-noise suppression, and wavelet compression are introduced. As a result, the effective frequency bandwidth of the newly processed data is broadened by approximately 10–16 Hz relative to the legacy dataset, and the imaging of small faults and narrow river-channel boundaries is significantly enhanced. On the interpretation side, ten sublayers within the first member of the Shaximiao Formation are correlated with high precision, yielding the identification of 41 fourth-order local structural units and 122 stratigraphic traps. Through seismic forward modeling and attribute optimization, a set of sensitive attributes suitable for thin-sandstone detection is established. These attributes enable fine-scale characterization of sand-body distributions within the shallow-water delta system, where fluvial control is pronounced, leading to the identification of 364 multi-phase superimposed channels. Based on attribute fusion, rock-physics-constrained inversion, and integrated hydrocarbon-indicator analysis, 147 favorable “sweet spots” are predicted, and six well locations are proposed. The study builds a reservoir-forming model of “deep hydrocarbon generation–upward migration, fault-controlled charging, structural trapping, and microfacies-controlled enrichment,” achieving high-fidelity imaging and quantitative prediction of tight sandstone reservoirs in the Shaximiao Formation. The results provide robust technical support for favorable-zone evaluation and subsequent exploration deployment in the Yingshan area. Full article

Ultra-high heat input welding offers high efficiency for large-scale offshore engineering, but excessive heat input can degrade low-temperature toughness. This study investigates the microstructural evolution and impact toughness of EH36 ship steel under high heat inputs (300–500 kJ/cm) using Gleeble-3500 thermal simulation, Charpy impact tests, and multi-scale characterization (OM, SEM, EBSD). Results show that impact toughness peaks at 400 kJ/cm, with surface and core energies reaching 343.33 J and 215.18 J, respectively. The optimal toughness is attributed to the formation of acicular ferrite and a high fraction of high-angle grain boundaries (up to 48.7%), which effectively inhibit crack propagation. These findings provide a practical basis for selecting heat input to balance welding efficiency and mechanical performance in marine steel fabrication. Full article

The oxygen evolution reaction (OER) is a critical anodic process in alkaline water electrolysis, and its catalytic performance can be effectively regulated through rational morphology engineering that governs active-site exposure, mass transport, and charge-transfer kinetics. Herein, we report a precursor-controlled synthesis of spinel NiCo₂O₄ (NCO) catalysts with tunable two-dimensional architectures for efficient alkaline OER. By employing hexamethylenetetramine (H) and urea (U) as precipitating agents, the NiCo₂O₄ catalysts with distinctly different nanosheet morphologies were directly grown on nickel foam. The NCO-H catalyst exhibits substantially enhanced OER activity by achieving lower overpotential of 259 mV, a smaller Tafel slope of 84 mV dec^–1, and higher turnover frequency compared to NCO-U catalyst. The superior OER performance is attributed to an ultrathin, highly interconnected nanosheet network that provides abundant accessible active sites, shortened ion-diffusion pathways, and accelerated interfacial charge transfer. Moreover, the optimized electrode demonstrates excellent durability (50 h) with negligible potential degradation after the partial surface transformation into an oxyhydroxide-rich active phase, while post-stability polarization and impedance analyses confirm the preservation of catalytic integrity. These findings highlight precursor-regulated morphology engineering as an effective strategy for optimizing the electrocatalytic performance of spinel oxides and establish NiCo₂O₄ as a robust, earth-abundant OER catalyst for alkaline water-splitting applications. Full article

Objectives: This study aimed to characterize the types and frequencies of sex chromosome aneuploidies (SCAs) detected through invasive prenatal testing, evaluate the concordance between non-invasive prenatal testing (NIPT) and confirmatory diagnostic methods, and assess the challenges faced during genetic counseling following SCA diagnosis. Study Design: A retrospective review was conducted on 842 prenatal samples collected between 2020 and 2024 in a tertiary private medical center. Samples included amniotic fluid, chorionic villi, and products of conception. Testing involved rapid QF-PCR for aneuploidy detection, followed by SNP-based chromosomal microarray analysis (CMA). NIPT results with high risk for sex chromosomes aneuploidies were correlated with invasive testing outcomes in 19 cases. Results: Sex chromosome aneuploidies were identified in 67 cases (7.96%), with Turner syndrome (45, X) being the most frequent (23 cases, including six mosaics), followed by Klinefelter syndrome (18 cases), 47, XYY (14 cases), and trisomy X (12 cases). Among 19 NIPT-tested cases, 10 were true positives, 5 false positives, and 4 false negatives, including two mosaic Turner syndrome cases undetected by NIPT. Discordances were attributed to factors such as mosaicism and placental anomalies. Conclusions: Prenatal diagnosis of SCAs via invasive testing remains crucial due to NIPT’s limited sensitivity for mosaicism and false positives. Comprehensive genetic counseling is essential to navigate diagnostic uncertainties and optimize prenatal management and postnatal outcomes. Full article

Granite residual soil (GRS) is highly susceptible to water-induced softening, posing significant risks of slope instability and collapse. Conventional impermeable grouting often exacerbates these hazards by blocking groundwater drainage. This study investigates the efficacy of a permeable water-reactive polyurethane (PWPU) in stabilizing GRS, aiming to resolve the conflict between mechanical reinforcement and hydraulic conductivity. Uniaxial compression tests were conducted on specimens with varying initial water contents (5%, 10%, and 15%) and PWPU contents (5%, 10%, and 15%). To reveal the multi-scale failure mechanism, synchronous acoustic emission (AE) monitoring and digital image correlation (DIC) were employed, complemented by scanning electron microscopy (SEM) for microstructural characterization. Results indicate that PWPU treatment significantly enhances soil ductility, shifting the failure mode from brittle fracturing to strain-hardening, particularly at higher moisture levels where failure strains exceeded 30%. This enhancement is attributed to the formation of a flexible polymer network that acts as a micro-reinforcement system to restrict particle sliding and dissipate strain energy. An optimal PWPU content of 10% yielded a maximum compressive strength of 4.5 MPa, while failure strain increased linearly with polymer dosage. SEM analysis confirmed the formation of a porous, reticulated polymer network that effectively bonds soil particles while preserving permeability. The synchronous monitoring quantitatively bridged the gap between internal micro-crack evolution and macroscopic strain localization, with AE analysis revealing that tensile cracking accounted for 79.17% to 96.35% of the total failure events. Full article

Nanocrystalline Fe-Gd-B alloys were successfully synthesized via Gd alloying in a binary Fe-B system using a single-roller melt-spinning technique. A systematic investigation of Gd content variation (0–4.35 at.%) reveals its critical role in tuning microstructure evolution, thermal stability, and magnetic properties. Crucially, the Fe_90.70Gd_2.32B_6.98 alloy ribbon exhibits optimized magnetic performance, achieving a high saturation magnetic induction (B_s) of 1.67 T and a low coercivity (H_c) of 2.737 kA/m. This enhancement is attributed to the suppression α-Fe grain growth through Gd-induced elevation of the thermal stability of the amorphous matrix, which confines the average crystallite size to 26.3 nm. The refined α-Fe phase contributes to elevated B_s through an increased ferromagnetic fraction, while its nanoscale grain structure, combined with wide magnetic domain configurations, effectively reduces H_c by limiting domain wall pinning sites. These findings establish that the synergistic effect of Gd alloying and Fe/B ratio adjustment is a viable strategy for designing high-performance Fe-based magnetic alloys. Full article

Apple aroma is an important factor influencing consumers’ preferences. To understand the overall flavor characteristics of apples (Ruixue, Liangzhi, Grystal Fuji, and Guifei), volatile compounds and aroma profiles were investigated by headspace–gas chromatography–ion mobility spectrometry (HS-GC-IMS) combined with stir bar sorptive extraction (SBSE) and gas chromatography–mass spectrometry (GC-MS). The results showed that a total of 56 aroma compounds were identified by SBSE-GC-MS, and 39 aroma-active compounds were screened out using aroma intensity (AI) and odor activity value (OAV). Aroma recombination experiments showed enhanced ‘fruity’ and ‘sweet’ notes, whereas ‘floral’, ‘woody’, and ‘green’ aromas were weaker compared to the Crystal Fuji sample. Additionally, GC-IMS coupled with principal component analysis (PCA) was used to distinguish the apple samples, and partial least squares regression (PLSR) was applied to explore the correlation between sensory attributes and characteristic aroma compounds. The results indicated that Crystal Fuji exhibited the greatest correlation with the “woody” attribute, and Ruixue was highly correlated with “fruity”, “green”, and “sour” attributes, while butanoic acid, β-damascenone, butyl acetate, pentyl acetate, furfuryl alcohol, γ-decalactone, and vanillin had a significant impact on the “flower” and “sweet” attributes of Guifei. This study clarified the characteristic aroma composition of the four apple cultivars, providing data support for apple flavor quality evaluation and cultivar optimization. Full article

Nanomaterial-based hole transport layers (HTLs) play a vital role in regulating interfacial charge extraction and recombination in perovskite solar cells (PSCs). To improve PSC efficiency, hydrothermally synthesized CeO₂@MoS₂ nanocomposites (CM NCs) were incorporated as an interfacial buffer layer into a NiO_X/MeO-2PACz HTL. The introduction of CM NCs induces strong interfacial interactions, where Mo sites in MoS₂ interact with NiO_X, modulating the Ni²⁺/Ni³⁺ ratio and reducing the interfacial trap density. Moreover, CeO₂ promotes the formation of oxygen vacancies, collectively improving the conductivity and hole transport capability of the NiO_X HTL. The MoS₂-grafted CeO₂ interlayer effectively tailors the interfacial energetics and creates an effective channel for hole transfer, thereby reducing open-circuit voltage (V_OC) loss and enhancing device performance. This interface modification efficiently enhances hole extraction, and non-radiative recombination is effectively suppressed at the NiO_X/MeO-2PACz/perovskite interface. Thereby, incorporating 2 vol% CM NCs into PSCs achieved a power conversion efficiency (PCE) of 17.93%, compared to 17.50% for a 1 vol% CM NCs-based device and 17.01% for the unmodified control device. The enhanced performance at the optimized CM NCs concentration is attributed to effective defect passivation, reduced V_OC loss, and improved interfacial band alignment, which together facilitate hole extraction and suppress non-radiative recombination. However, excessive CM NCs incorporation (4 vol%) leads to increased interfacial resistance, partial hole blocking effects associated with the n-type nature of CeO₂, and aggravated recombination, resulting in degraded device performance. These results demonstrate that precise control over CM NCs interlayer thickness and concentration is critical for maximizing device performance, providing a robust strategy for designing high-efficiency and stable NiO_X-based PSCs and advancing nanocomposite-enabled interfacial engineering for photovoltaic applications. Full article

A newly commercialized single-row multi-fan autonomous unmanned ground vehicle (UGV) sprayer, for use in trellised tree fruit crops, was tested to better understand air and spray patterns prior to wide-scale adoption in the modern apple orchard systems typical to Washington State. This sprayer was equipped with five brown and yellow Albuz ATR80 nozzles per fan (QM-420, Croplands Quantum). The fans were installed in a Q8 configuration, with eight fans (four on each side) staggered near the front and back as a stack to increase vertical span. Air velocity and spray delivery patterns of the commercialized sprayer unit were assessed in laboratory using a customized smart spray analytical system. Previous field trails of this sprayer unit revealed a hardware issue with electric proportional valve controls in fan-nozzle assembly, resulting in uneven spray deposition across V-trellised canopy. Post issue resolution, the sprayer characterization data showed an average Symmetry of 91%, and 84% for air velocity and spray volume delivery on either side. An average Uniformity of 57% and 48%, respectively was recorded for pertinent sprayer attributes across the spray height. Overall, after optimization, the UGV sprayer is suitable for efficient agrochemical application in modern orchard systems. Further evaluation of labor savings, biological efficacy gains from autonomous operation, and a full economic analysis would better inform grower adoption. Commercial viability of this UGV sprayer could also be improved by added features such as variable-rate application enabled by real-time crop sensing or task-map integration. Full article

Wastewater treatment plants represent an important point source of microplastics (MPs) entering aquatic environments, raising increasing concerns regarding ecosystem integrity and potential risks to human health. Improving the removal efficiency of MPs during wastewater treatment is therefore of both environmental and technological significance. Polyaluminum chloride (PAC), polyferric sulfate (PFS), and polyacrylamide (PAM) were applied to remove MPs by coagulation, with particular emphasis on the effects of PAM type (cationic, anionic, and non-ionic). The optimal removal efficiency achieved by PAC alone for polystyrene was 55.00 ± 3.54% at a dosage of 300 mg L⁻¹, which increased significantly to 87.50 ± 1.87% with the addition of cationic PAM. Similarly, MPs removal by PFS increased from 33.75 ± 1.77% at 160 mg L⁻¹ to 62.50 ± 3.53% when combined with cationic PAM. Overall, PAC-based coagulation exhibited higher MPs removal efficiency than PFS, and cationic PAM outperformed anionic and non-ionic PAM, likely attributable to electrostatic interactions with negatively charged MPs in wastewater systems. In addition, PAC/PAM coagulation enabled effective removal of multiple MPs types while simultaneously enhancing phosphate removal, highlighting its potential for the integrated control of MPs and phosphate pollution in wastewater systems. Full article

Art museums play a critical role in sustaining cultural heritage, promoting lifelong learning, and supporting sustainable urban and tourism development. In this study, we identify the key attributes of art museums that shape visitor satisfaction and clarify how each attribute contributes to satisfaction or dissatisfaction. By drawing on the Kano model, we argue that not all attributes play the same role in influencing visitor evaluations, which holds important implications for the sustainable management of cultural institutions. To empirically test this, we analyze user-generated online reviews from a major immersive museum in South Korea using a two-stage approach: first, the main experiential attributes were extracted through topic modeling; second, the relationship between topic mentions and overall review ratings was evaluated through regression analysis. The findings reveal eight core attributes of the art museum experience, with one functioning as a satisfier and the others as dissatisfiers, demonstrating the asymmetric effects proposed by the Kano model. This study contributes to sustainability research in hospitality and tourism by conceptualizing art museums as service products with distinct attribute structures and by showing how optimized management of these attributes can strengthen the long-term sustainability of cultural tourism destinations. Practically, our results provide guidance for museum managers on which attributes require strategic prioritization to enhance visitor satisfaction and support sustainable operational practices. Full article

The development of efficient and durable oxygen evolution reaction (OER) catalysts from earth-abundant materials is essential for advancing alkaline water electrolysis. Herein, nanograss-like CoMn₂O₄ electrode films are directly grown on stainless-steel substrates via a temperature-controlled hydrothermal approach, and their OER performance is systematically investigated. The CoMn₂O₄ obtained at 120 °C (CMO-120) delivers the best catalytic activity in 1.0 M KOH, requiring an overpotential of 292 mV at 10 mA cm⁻², which is lower than those synthesized at 150 (CMO-150) and 90 °C (CMO-90). Notably, activity of CMO-120 becomes even more pronounced at elevated current densities, achieving the low overpotential of 434 mV even at 300 mA cm⁻², substantially outperforming both CMO-90 and CMO-150 electrodes. The enhanced activity is attributed to an interconnected nanograss architecture with mixed Co²⁺/Co³⁺ and Mn²⁺/Mn³⁺ redox couples and abundant defect-related oxygen species, which result in increased electrochemically active surface area and improved charge transportation throughout the nanograss architecture that facilitate OH⁻ adsorption and OER intermediate transformation. Furthermore, CMO-120 demonstrates excellent durability (100 h) after electro-oxidation-induced surface activation. These findings highlight precise temperature regulation as an effective strategy for optimizing Mn-Co spinel for efficient alkaline OER applications. Full article

The intensifying pressures of urbanization and climate change on coastal zones necessitate a holistic understanding of the interplay between human activity and ecological integrity for sustainable development. However, prevailing methods for assessing coastal vibrancy often overlook direct measures of human presence and fail to quantitatively capture its complex relationship with ecological vulnerability. To address these gaps, this study develops a novel multi-dimensional assessment framework for Coastal Landscape Vibrancy (CLV) and empirically examines its interaction with ecological vulnerability factors in Beihai, China. Moving beyond built-environment-centric approaches, our framework integrates the ‘Crowd’ dimension, directly quantified using Baidu Heat Index data, with the ‘Place’ dimension, characterized by urban features, natural attributes, and visual experience. Principal Component Analysis (PCA) was employed to objectively weight these indicators and construct a composite CLV index. We then applied multiple linear regression to analyze the influence of ecological factors constructed based on the Sensitivity-Resilience-Pressure (SRP) model. The results revealed that vibrancy was highly concentrated in urban cores and exhibited significant spatiotemporal variations. Regression analysis revealed that while ecological quality factors like green coverage (β = 0.236, p < 0.001) positively influenced vibrancy, anthropogenic stressors such as slope (β = −0.457, p < 0.001) and the impervious surface index (β = −0.092, p < 0.001) had significant negative impacts, highlighting a critical trade-off between human activity and ecological conditions. The findings provide a quantitative, evidence-based foundation for spatial planning, demonstrating that sustainable coastal vibrancy is achieved through a balanced integration of human activity and ecological conservation, rather than through unchecked development. This framework offers critical insights for formulating strategies that simultaneously enhance ecological resilience and optimize human service facilities. Full article