Search Results (167)

Focusing on the West African Craton (WAC) as a test bed, this protocol-oriented scoping review synthesizes indicators for orogenic gold and translates them into an auditable, map-first checklist that separates Fertility and Preservation, while deliberately deferring any performance estimation to a blinded, out-of-sample evaluation. There is a need for a transparent, auditable, and field-ready framework that integrates geological, structural, geophysical, and geochemical evidence. We (i) synthesize the state of knowledge into a map-first, reproducible targeting checklist, (ii) formalize an indicator decision matrix that separates Fertility from Preservation factors, and (iii) specify a deferred, out-of-sample evaluation protocol to quantify performance. We conduct a Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR)-style scoping review (2010–2025) and codify commonly used indicators (e.g., transpressional jogs, lineament density, proximity to tonalite-trondhjemite-granodiorite (TTG)/tonalite contacts, Sr/Y proxies). Indicators are operationalized as auditable pass/fail rules and assembled into a decision matrix with explicit uncertainty handling and risk logging. We further define a deferred evaluation protocol using classification and ranking metrics (receiver operating characteristic (ROC) and precision–recall (PR) curves, odds ratios), ablation/sensitivity tests, and district-level threshold calibration. We deliver (1) a unified, auditable checklist with default (tunable) thresholds; (2) an indicator decision matrix that disentangles Fertility vs. Preservation signals; and (3) a deferred evaluation protocol enabling a reproducible, out-of-sample assessment without inflating apparent performance. All numerical thresholds reported here are explicit placeholders that facilitate transparency and auditability; they are not optimized. A properly blocked train/validation/test scheme, operating-point selection criteria, null models, and uncertainty procedures are prespecified for future evaluation. By publishing the checklist, data lineage, and audit-log schema now—without performance claims—we enable reproducible adoption and stress-test the framework ahead of calibration. Full article

Ex-ante appraisal of agricultural policy needs a transparent way to trace how sectoral interventions translate into production. We study the Polish CAP case and ask how much selected actions matter for livestock sectors. We assembled intervention-level budgets from the CAP Strategic Plan for Poland (2023–2027) and sectoral final output for milk, pigs, beef and poultry from Statistics Poland/Eurostat. We built matrices that map actions to sectors, normalized transfers by sectoral output, and separated dedicated from spillover effects. We report two cross-sections (2024, 2028) and a robustness test that perturbs I 1–I 2 allocation shares by ±10% under fixed envelopes. Horizontal income support dominates. In 2024, the cumulative effect of all analyzed actions equaled 16.68% of final output in milk, 14.43% in beef, 5.15% in pigs and 4.29% in poultry; by 2028, these values ease to 15.07%, 12.93%, 3.84% and 4.15%. Coupled payments to cows and young cattle add contributions in milk and beef. The ±10% reweighting of I 1–I 2 keeps the sector ranking unchanged; level changes are moderate (about 0.4–1.2 percentage points). A compact matrix approach provides a replicable map from interventions to sectors and highlights the preponderance of horizontal income support. The pattern—strongest relative support in milk and beef—appears robust to plausible allocation uncertainty. The main limitation is the use of final output as a revenue proxy; extending the matrix to all CAP actions and adding price–quantity feedback would be a natural next step. Policy implication: modest rebalancing of I 1–I 2 shares will not overturn sectoral exposure, but adjustments targeted at beef move levels the most. Full article

The central purpose of this study is to develop and validate an advanced numerical model capable of simulating the vibrational behavior of the operator’s seat in a tractor-type agricultural vehicle designed for operation in protected horticultural environments, such as vegetable greenhouses. The three-dimensional (3D) model of the seat was created using SolidWorks 2023, while its dynamic response was investigated through Finite Element Analysis (FEA) in Altair SimSolid, enabling a detailed evaluation of the natural vibration modes within the 0–80 Hz frequency range. Within this interval, eight significant natural frequencies were identified and correlated with the real structural behavior of the seat assembly. For experimental validation, direct time-domain measurements were performed at a constant speed of 5 km/h on an uneven, grass-covered dirt track within the research infrastructure of INMA Bucharest, using the TE-0 self-propelled electric tractor prototype. At the operator’s seat level, vibration data were collected considering the average anthropometric characteristics of a homogeneous group of subjects representative of typical tractor operators. The sample of participating operators, consisting exclusively of males aged between 27 and 50 years, was selected to ensure representative anthropometric characteristics and ergonomic consistency for typical agricultural tractor operators. Triaxial accelerometer sensors (NexGen Ergonomics, Pointe-Claire, Canada, and Biometrics Ltd., Gwent, UK) were strategically positioned on the seat cushion and backrest to record accelerations along the X, Y, and Z spatial axes. The recorded acceleration data were processed and converted into the frequency domain using Fast Fourier Transform (FFT), allowing the assessment of vibration transmissibility and resonance amplification between the floor and seat. The combined numerical–experimental approach provided high-fidelity validation of the seat’s dynamic model, confirming the structural modes most responsible for vibration transmission in the 4–8 Hz range—a critical sensitivity band for human comfort and health as established in previous studies on whole-body vibration exposure. Beyond validating the model, this integrated methodology offers a predictive framework for assessing different seat suspension configurations under controlled conditions, reducing experimental costs and enabling optimization of ergonomic design before physical prototyping. The correlation between FEA-based modal results and field measurements allows a deeper understanding of vibration propagation mechanisms within the operator–seat system, supporting efforts to mitigate whole-body vibration exposure and improve long-term operator safety in horticultural mechanization. Full article

Building on the early investigation by Sidney W. Fox that dry-heated amino acids can spontaneously form microspheres, this research studies the self-organization of glycine and alanine with hydrogen in a liquid system. This study aimed to investigate the spontaneous formation of membraneless, microscale amino acid assemblies under simulated prebiotic hydrothermal conditions, such as hot mineral sources and ponds. Aqueous solutions of glycine and alanine were prepared in a hydrogen-rich mineral buffer and thermally incubated at 75 °C. Phase-contrast microscopy, transmission electron microscopy (TEM), and molecular modeling were employed to analyze the morphology and internal organization of the resulting structures. Microscopy revealed that zwitterionic glycine and alanine spontaneously self-organize into spherical microspheres (~12 µm), in which the charged –NH₃⁺ and –COO⁻ groups orient outward, while the hydrophobic methyl groups of alanine point inward, forming a stabilized internal core. The primary studies were performed with hot mineral water from Rupite, Bulgaria, at 73.4 °C. The resulting osmotic pressure difference Δπ ≈ 2490 Pa, derived from the van’t Hoff equalization. This suggests a chemically asymmetric system capable of sustaining directional water flux and passive molecular enrichment. The zwitterionic nature of glycine and alanine, which possesses both –NH₃⁺ and –COO⁻ groups, supports the formation of microspheres in our experiments. Under conditions with hot mineral water and hydrogen acting as a reducing agent in the primordial atmosphere, these amino acids self-organized into dense interfacial microspheres. These findings support the idea that thermally driven, zwitterion-mediated aggregation of simple amino acids, such as glycine and alanine, with added hydrogen, could generate membraneless, selectively organized microenvironments on the early Earth. Such microspheres may represent a plausible intermediate between dispersed organisms and microspheres. Full article

Noccaea species (formerly Thlaspi) are Brassicaceae plants renowned for their capacity to hyperaccumulate zinc (Zn), cadmium (Cd), and nickel (Ni), which has made them model systems in studies of metal tolerance, phytoremediation, and plant adaptation to extreme environments. While their physiological and genetic responses to metal stress are relatively well characterised, the extent to which these traits influence microbiome composition and function remains largely unexplored. These species possess compact genomes shaped by ancient whole-genome duplications and rearrangements, and such genomic traits may influence microbial recruitment through changes in secondary metabolism, elemental composition, and tissue architecture. Here, we synthesise the current findings on how genome size, metal hyperaccumulation, structural adaptations, and glucosinolate diversity affect microbial communities in Noccaea roots and leaves. We review evidence from bioimaging, molecular profiling, and physiological studies, highlighting interactions with bacteria and fungi adapted to metalliferous soils. At present, the leaf microbiome of Noccaea species remains underexplored. Analyses of root microbiome, however, reveal a consistent taxonomic core dominated by Actinobacteria and Proteobacteria among bacterial communities and Ascomycetes, predominantly Dothideomycetes and Leotiomycetes among fungi. Collectively, these findings suggest that metal-adapted microbes provide several plant-beneficial functions, including metal detoxification, nutrient cycling, growth promotion, and enhanced metal extraction in association with dark septate endophytes. By contrast, the status of mycorrhizal associations in Noccaea remains debated and unresolved, although evidence points to functional colonisation by selected fungal taxa. These insights indicate that multiple plant traits interact to shape microbiome assembly and activity in Noccaea species. Understanding these dynamics offers new perspectives on plant–microbe co-adaptation, ecological resilience, and the optimisation of microbiome-assisted strategies for sustainable phytoremediation. Full article

AI-integrated robotics in Industry 5.0 demands advanced manufacturing systems capable of autonomously interpreting complex geometries and dynamically adjusting machining strategies in real time—particularly when dealing with aerospace components featuring large-curvature surfaces. Large-curvature aerospace components present significant challenges for precision drilling due to surface-normal deviations caused by curvature, roughness, and thin-wall deformation. This study presents a robotic drilling system that integrates adaptive PCA-based surface normal estimation with in-process pre-drilling correction and post-drilling verification. This system integrates a 660 nm wavelength linear laser projector and a 1.3-megapixel industrial camera arranged at a fixed 30° angle, which project and capture structured-light fringes. Based on triangulation, high-resolution point clouds are reconstructed for precise surface analysis. By adaptively selecting localized point-cloud regions during machining, the proposed algorithm converts raw measurements into precise normal vectors, thereby achieving an accurate solution of the normal direction of the surface of large curvature parts. Experimental validation on a 400 mm-diameter cylinder shows that using point clouds within a 100 mm radius yields deviations within an acceptable range of theoretical normals, demonstrating both high precision and reliability. Moreover, experiments on cylindrical aerospace-grade specimens demonstrate normal direction accuracy ≤ 0.2° and hole position error ≤ 0.25 mm, maintained across varying curvature radii and roughness levels. The research will make up for the shortcomings of existing manual drilling methods, improve the accuracy of hole-making positions, and meet the high fatigue service needs of aerospace and other industries. This system is significant in promoting the development of industrial automation and improving the productivity of enterprises by improving drilling precision and repeatability, enabling reliable assembly of high-curvature aerospace structures within stringent tolerance requirements. Full article

Gout and calcium pyrophosphate crystal deposition disease (CPPD) are frequently associated with comorbid disorders, including coronary artery disease and osteoarthritis, in which ectopic calcification with basic calcium phosphate crystals commonly affects arteries and articular cartilage, respectively. Accepting the 2024 G-CAN Gold Medal, I review my research philosophy for translational etiopathogenesis investigation in gout and CPPD, atherosclerosis, responses to arterial injury, and osteoarthritis. Since molecular homeostasis points to pathophysiology and vice versa, I have followed selected molecular players and pathways to phenotypes. Typically, behind each disease target is another target. Illuminating passageways between etiopathogenic pathways is especially productive when using approaches beyond conventional “omics” to reveal the impact of specific post-translational protein modifications, and changes in protein conformation, complex assembly, and interactomes. Highlighting these concepts, I review my past studies on specific molecular pathways, and current perspectives for the following: (i) PP_i, NPP1, ANKH, and transglutaminase 2 (TG2); (ii) relationships between NPP1, ANKH, Vanin-1 Pantetheinase, and ectopic chondrogenesis; (iii) intersections between adenosine, AMPK, CXCL8 and its receptor CXCR2, the receptor for advanced glycation endproducts (RAGE) and chondrocyte hypertrophy; (iv) lubricin homeostasis and proteolysis; (v) receptor for advanced glycation endproducts (RAGE) and TG2-catalyzed post-translational calgranulin modification; (vi) complement activation and C5b-9 assembly, and the nucleotide-bound conformation of TG2. The inescapable conclusion is that these molecular pathways tightly knit crystal arthropathy with both arterial and osteoarthritis comorbidity. Full article

This study proposes a novel computational method, employing the integral dynamics of multibody systems to simulate the transverse vibrations of the rotor in a cantilever-type centrifugal pump. This method was applied to the kinematic assembly of the rotor and its supports, with the latter modeled as springs possessing stiffness and damping properties equivalent to those of real bearings supporting the shaft in an actual design. To investigate transverse vibrations within the system, three key observation points were defined—at the locations of the left and right bearings, as well as at the rotor’s center of mass—to allow for a thorough dynamic analysis. Additionally, the influence of motor rotational speed and the impeller’s eccentricity on the transverse vibrations of the supports and the shaft was examined. The results have revealed that transverse vibrations significantly affect the system’s dynamics at lower rotational speeds, leading to the classification of the shaft as flexible. As the rotational speed increases, the system exhibits enhanced dynamic stability. Furthermore, it was found that for impellers with a diameter less than 300 mm, the unbalanced forces are negligible and can be disregarded in pump design. To reduce vibration levels, an elastic damping ring was selected and incorporated into the system. This novel method provides an effective tool for analyzing the transverse vibrations of centrifugal pump rotors and for optimizing vibration mitigation strategies. Full article

Metal–Organic Frameworks (MOFs) have emerged as advanced porous crystalline materials due to their highly ordered structures, ultra-high surface areas, fine-tunable pore sizes, and massive chemical diversity. These features, arising from the coordination between an almost unlimited number of metal ions/clusters and organic linkers, have resulted in significant interest in MOFs for applications in gas storage, catalysis, sensing, energy, and biomedicine. Beyond their stand-alone properties and applications, recent research has increasingly explored the integration of MOFs with other substrates, particularly electrodes, polymeric thin films, and glass surfaces, to create synergistic effects that enhance material performance and broaden application potential. Coating MOFs onto these substrates can yield significant benefits, including, but not limited to, improved sensitivity and selectivity in electrochemical sensors, enhanced mechanical and separation properties in membranes, and multifunctional coatings for optical and environmental applications. This review provides a comprehensive and up-to-date summary of recent advances (primarily from the past 3–5 years) in MOF coating techniques, including layer-by-layer assembly, in situ growth, and electrochemical deposition. This is followed by a discussion of the representative applications arising from MOF-substrate coating and an outline of key challenges and future directions in this rapidly evolving field. This article aims to serve as a focused reference point for researchers interested in both fundamental strategies and applied developments in MOF surface coatings. Full article

Aiming to address the problem of accuracy degradation in Delta robots caused by machining accuracy, assembly precision, etc., this paper corrects the robot’s driving angles to achieve error compensation and designs a compensation algorithm based on particle swarm optimization (PSO) and BP neural network. In terms of algorithm improvement, the inertia weight and learning factors of the PSO algorithm are optimized to effectively enhance the global search ability and convergence performance of the algorithm. Additionally, the core mechanisms of genetic algorithms, including selection, crossover, and mutation operations, are introduced to improve algorithm diversity, ultimately proposing an improved PSO-GA-BP error compensation algorithm. This algorithm uses the improved PSO-GA algorithm to optimize the optimal correction angles and trains the BP network with the optimized dataset to achieve predictive compensation for other points. The simulation results show that the comprehensive error of the robot after compensation by this algorithm is reduced by 83.8%, verifying its effectiveness in positioning accuracy compensation and providing a new method for the accuracy optimization of parallel robots. Full article

Magnesium ions (Mg²⁺) play an important role in animal health, with their concentration in the bloodstream serving as a key indicator for hypomagnesemia diagnosis. In this study, a flexible hydrophobic paper-based microfluidic field-effect biosensor was developed for point-of-care Mg²⁺ detection, which integrated flexible hydrophobic paper, semiconducting single-walled carbon nanotubes (SWNTs) and a Mg²⁺-specific RNA-cleaving DNAzyme(RCD)-based DNA nanostructure. Flexible hydrophobic paper was synthesized by using cellulose paper and octadecyltrichlorosilane, improving mechanical strength and decreasing biological interference. To achieve high sensitivity, the Mg²⁺-specific RCD was functionalized with SWNTs, and then repeatedly self-assembled two different Y-shaped DNAs to construct a DNA nanostructure based on a similar DNA origami technique. This proposed biosensor exhibited a linear detection range from 1 μM to 1000 μM, with a detection limit of 0.57 μM, demonstrating its great stability, selectivity, and anti-interference performance. This innovative design offers promising potential for Mg²⁺ monitoring in real applications. Full article

The Spodoptera litura, a Lepidopteran pest known for its high fecundity, undergoes a complete metamorphosis, including a distinctive process during which the male testes fuse from two separate organs into a single entity, significantly enhancing its fertility. To elucidate the molecular mechanisms underlying this testicular fusion, this study employed an integrated multi-omics approach to investigate concurrent changes at the transcriptomic and proteomic levels. We identified a series of synchronized alterations on the peritestic larval membrane, including heme binding, peptidase activity, hydrolase activity, metal ion transport, redox reactions, and chitin metabolism, all of which are substantially enriched at specific temporal points during testicular fusion. Nine genes/proteins co-expressed at the mRNA and protein levels were selected for targeted quantitative proteomics (PRM) and quantitative PCR (qPCR) validation, leading to the identification of five genes potentially involved in the testicular fusion process: Sl3030, ARCP, PSLRE, Obstructor-E, and Osris9B. Notably, the gene Sl3030, once knocked out, not only disrupted the normal fusion process but also resulted in reduced testis size, thickened peritestic membranes, and abnormal sperm development. Transcriptomic sequencing of the Sl3030 knockout mutant revealed its primary influence on the fusion process by affecting the assembly of the microtubule system and cytoskeleton. This research, for the first time, provides a multi-omics perspective on the response of key signaling pathways and molecular changes during the testicular fusion of S. litura and validates the role of the previously uncharacterized gene Sl3030 in this process, offering valuable insights into the complex mechanisms of testicular fusion in this species. Full article

(1) Burn injuries demand multidisciplinary, evidence-based care, yet the extensive literature complicates timely decision making. Retrieval-augmented generation (RAG) synthesizes research while addressing inaccuracies in pretrained models. However, citation bias in sourcing for RAG often prioritizes highly cited studies, overlooking less-cited but valuable research. This study examines RAG’s performance in burn management, comparing citation levels to enhance evidence synthesis, reduce selection bias, and guide decisions. (2) Two burn management datasets were assembled: 30 highly cited (mean: 303) and 30 less-cited (mean: 21). The Gemini-1.0-Pro-002 RAG model addressed 30 questions, ranging from foundational principles to advanced surgical approaches. Responses were evaluated for accuracy (5-point scale), readability (Flesch–Kincaid metrics), and response time with Wilcoxon rank sum tests (p < 0.05). (3) RAG achieved comparable accuracy (4.6 vs. 4.2, p = 0.49), readability (Flesch Reading Ease: 42.8 vs. 46.5, p = 0.26; Grade Level: 9.9 vs. 9.5, p = 0.29), and response time (2.8 vs. 2.5 s, p = 0.39) for the highly and less-cited datasets. (4) Less-cited research performed similarly to highly cited sources. This equivalence broadens clinicians’ access to novel, diverse insights without sacrificing quality. As plastic surgery evolves, RAG’s inclusive approach fosters innovation, improves patient care, and reduces cognitive burden by integrating underutilized studies. Embracing RAG could propel the field toward dynamic, forward-thinking care. Full article

This paper proposes a kinematic modeling method for robotic manipulators by extracting the modified Denavit–Hartenberg (MDH) parameters using line geometry. For single-branched manipulators, various joint axes can be represented as lines using Plücker coordinates. The forward kinematics is derived by performing the product of matrices which are the exponential maps lifted from two kinds of exponential coordinates using the MDH parameters. For extracting MDH parameters, line geometry systematically analyzes the following: (1) the closest point between a point and line, (2) the closest distance and twist angle between two lines, (3) the common perpendicular line and its intersection points, and (4) classifies line relationships into collinear, distant parallel, intersected, and skewed cases. For each case, five parameters including twist angle, closest distance, common perpendicular direction vector, and both feet on a common perpendicular line are sequentially computed as results of the line geometry block. Finally, the aforementioned line geometry blocks are utilized to extract the four MDH parameters according to their definitions. The effectiveness of the proposed algorithm is verified by four examples including a typical Selective Compliance Assembly Robot Arm (SCARA) robot and three different commercial manipulators. Full article

The working accuracy of space optical payloads, sensitive components, greatly depends on the pointing accuracy and stability of the platform. This article establishes a mathematical model for relative position and attitude measurement based on PSD and eddy current and analyzes the failure modes under the measurement models. Through model derivation, it can be concluded that the position and attitude measurement system has high redundancy; in the event of sensor failure in the horizontal or vertical direction, relative position and attitude measurement and resolution can still be completed, which solves the relative measurement problem of position and attitude measurement of the space Maglev vibration isolation control system, providing high-precision closed-loop control for the control system to achieve high-precision pointing and stability. In response to the requirements of high-precision non-contact displacement and attitude measurement, eddy current sensors were selected, and a sensor circuit box was designed. The testing and calibration system adopts an eight-bar Maglev layout, and the actuator has unidirectional dual-mode output. The actuator adopts a double closed magnetic circuit structure, and the coil adopts a winding single-coil structure. The system includes a multi-degree-of-freedom high-precision coil spatial pose automatic positioning platform, a strong magnetic structure, strong uniform magnetic field magnetization, an integrated assembly testing platform, etc. According to the test data, the driver has strong linearity in both low- and high-current ranges. The relative output error in the low-current range does not exceed 0.1 mA, and the relative output error in the high-current range does not exceed 2 mA. After fitting and calibration, it can meet the design requirements. Within redundant designing, fault mode analyzing, and system testing, the relative measurement system can ensure the working accuracy of the optical payload of the spacecraft, which reaches the advanced level in the field. Full article