Search Results (248)

Mechanobiology plays a pivotal role in skeletal development and bone remodeling. Mechanical signals such as matrix stiffness, fluid shear stress, and hydrostatic pressure activate the Runt-related transcription factor 2 (RUNX2) bone-specific transcription factor through pathways including the mitogen-activated protein kinase (MAPK) signaling cascade and yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ) effectors. RUNX2 itself affects chromatin remodeling and nuclear architecture via Lamin A/C and Nesprin 1, thereby directing osteogenic differentiation. Thus, RUNX2 acts both as a mechanosensor and mechanoregulator, whereas RUNX2’s mechanosensitivity has been leveraged as a target to achieve bone regeneration. Notably, post-translational modifications and epigenetic alterations can orchestrate this regulation, integrating metabolic and circadian signals. However, due to RUNX2’s nuclear localization, its targeting remains a challenging issue. To this end, indirect targeting, through mammalian/mechanistic target of rapamycin complex 1 (mTORC1) or microRNAs (miRNAs), offers new strategies to employ biomechanics in an attempt to intervene with bone diseases driven by mechanical cues or degeneration, and ultimately repair and regenerate the damaged tissues. Herein we critically elaborate upon molecular aspects of RUNX2 regulation towards exploitation at the clinical level. Full article

This study employs physical experiments and the RFPA3D numerical method to investigate the fracture evolution of rocks containing a central hole with symmetrically arranged double cracks (seven inclination angles β) under biaxial compression. The results demonstrate that peak stress and strain exhibit nonlinear increases with rising β. Tensile–shear failure dominates at lower angles (β = 0–60°), characterized by secondary crack initiation at defect tips and wing/anti-wing crack development at intermediate angles (β = 45–60°). At higher angles (β = 75–90°), shear failure prevails, governed by crack propagation along hole walls. When β exceeds 45°, enhanced normal stress on crack planes suppresses mode II propagation and secondary crack formation. Elevated lateral pressures (15–20 MPa) significantly alter failure patterns by redirecting the maximum principal stress, causing cracks to align parallel to this orientation and driving anti-wing cracks toward specimen boundaries. Three-dimensional analysis reveals critical differences between internal and surface fracture propagation, highlighting how penetrating cracks around the hole crucially impact stability. This study provides valuable insights into complex fracture mechanisms in defective rock masses, offering practical guidance for stability assessment in underground mining operations where such composite defects commonly occur. Full article

Scrap tire-derived geomaterial has been gaining attention recently as an alternative material for improving the ground. This paper presents a fundamental experimental investigation into sand–rubber mixtures using hollow cylinder torsional shear apparatus, with the aim of enhancing our understanding of the integrated effects of rubber content and cyclic stress ratio (CSR) on the liquefaction characteristics of the mixtures. The results show that the incorporation of granular rubber into sand not only reduces excess pore water pressure during cyclic loading but also alters the generation mode of pore water pressure. The liquefaction resistance of the sand–rubber mixture increases significantly when the rubber gravimetric proportion exceeds 10%. The energy dissipation per loading cycle decreases with increasing rubber content, whereas the cumulative dissipative energy exhibits an opposite trend, showing a positive correlation with rubber content. In addition, this rubber-enhanced effect shows CSR dependence; the cumulative energy dissipation significantly diminishes at a high CSR. Therefore, the effect of granular rubber addition to sand on pore water pressure tends to become more pronounced at higher rubber contents. Full article

The vibrational response and stress of satellites subjected to acoustic excitation are essential components to consider in the design process of large satellite constructions. To precisely forecast the vibrational response and stress subjected to acoustic excitation of a large satellite honeycomb sandwich plate, this article employs finite element modeling software to create a finite element model of satellite, equivalent honeycomb panels to orthotropic shear plates of identical stiffness and dimensions, and convert the acoustic excitation into random pressure on the surface of the flat plate, applying it to the surface of the satellite, then the vibration response and stress analysis subjected to acoustic excitation can be performed. The honeycomb structure collapsed after noise testing on a specific model of a large satellite, the aforementioned technique for response and stress validation was employed: the stress simulation analysis revealed that the maximum shear stress at the fracture site of the honeycomb panel was 0.45 MPa, exceeding the ultimate stress value of 0.44 MPa for the sparse honeycomb core at that location, leading to the collapse and fracture of the honeycomb panel. To resolve this matter, altering the honeycomb core design framework, a local dense honeycomb structure was implemented. The simulated shear stress of the honeycomb core is 0.54 MPa, which is below the stress limit of 2.43 MPa for the dense honeycomb core. To verify the feasibility of the plan, the noise test was conducted once again. Owing to the incapacity to test the shear stress of the honeycomb core, conducted strain testing on the surface of the honeycomb collapse and derive stress results by calculation, the test results deviate from the modeling values by 8.92%. And the maximum discrepancy between the simulated noise response and the experimental noise response is 0.58 g, validated the efficacy and precision of the simulation method, and successfully resolved the issue of damage to satellite honeycomb panels. This simulation method can precisely forecast the vibrational response and stress of satellites subjected to acoustic excitation. Full article

Background/Objectives: The aberrant subclavian artery (ASA) represents the most common congenital anomaly of the aortic arch, and is frequently associated with a Kommerell diverticulum, an aneurysmal dilation at the anomalous vessel origin. This condition carries a significant risk of rupture and dissection, and growing evidence indicates that local hemodynamic alterations may contribute to its development and progression. Computational Fluid Dynamics (CFD) provides a valuable non-invasive modality to assess biomechanical stresses and elucidate the pathophysiological mechanisms underlying these vascular abnormalities. Methods: In this study, twelve thoracic CT angiography scans were analyzed: six from patients with ASA and six from individuals with normal aortic anatomy. CFD simulations were performed using OpenFOAM, with standardized boundary conditions applied across all cases to isolate the influence of anatomical differences in flow behavior. Four key hemodynamic metrics were evaluated—Wall Shear Stress (WSS), Oscillatory Shear Index (OSI), Drag Forces (DF), and Turbulent Viscosity Ratio (TVR). The aortic arch was subdivided into Ishimaru zones 0–3, with an adapted definition accounting for ASA anatomy. For each region, time- and space-averaged quantities were computed to characterize mean values and oscillatory behavior. Conclusions: The findings demonstrate that patients with ASA exhibit markedly altered hemodynamics in zones 1–3 compared to controls, with consistently elevated WSS, OSI, DF, and TVR. The most pronounced abnormalities occurred in zones 2–3 near the origin of the aberrant vessel, where disturbed flow patterns and off-axis mechanical forces were observed. These features may promote chronic wall stress, endothelial dysfunction, and localized aneurysmal degeneration. Notably, two patients (M1 and M6) displayed particularly elevated drag forces and TVR in the distal arch, correlating with the presence of a distal aneurysm and right-sided arch configuration, respectively. Overall, this work supports the hypothesis that aberrant hemodynamics contribute to Kommerell diverticulum formation and progression, and highlights the CFD’s feasibility for clarifying disease mechanisms, characterizing flow patterns, and informing endovascular planning by identifying hemodynamically favorable landing zones. Full article

Microchannels can create disturbed flow patterns by altering pressure gradients, shear forces, and flow symmetry, which are essential in the design of microfluidic devices and, hence, blood-contacting devices. The effect of asymmetric stenosis on pressure, wall shear stress, and velocity in rectangular and circular microchannels with same operating conditions was analyzed in this study using three-dimensional (3D) steady laminar computational fluid dynamics (CFD) simulations. Asymmetric flow patterns induced by asymmetric stenosis are of particular importance and remain underexplored, especially in the context of multiple constrictions. This is, to our knowledge, is the first systematic CFD comparison of multiple asymmetric stenoses in circular microchannels directly contrasted with rectangular and single-stenosis cases under identical settings. Several parameters, such as wall shear stress (WSS), pressure, and velocity distributions, were analyzed in various stenotic and non-stenotic geometries. These microchannel models, while not reflecting real blood vessels themselves nor exhibiting wall compliance, pulsatility, or non-Newtonian rheology, replicate important mechanical characteristics of stenosis-mediated flow disturbance. Single and multiple asymmetric stenoses create flow patterns that are similar to those of vascular pathologies. For this reason, these channels should be considered as simplified device-scale models of vascular phenomena as opposed to realistic, in vitro vascular models. The results showed that asymmetric stenosis creates asymmetric velocity peaks and elevated WSS, which are more evident in the case of circular configurations with double asymmetric stenosis. The findings will help design microfluidic devices that mimic unstable flow characteristics that occur in stenotic conditions, and assist in testing clinical devices. In this study, two fabrication-ready microchannel designs under fixed operating conditions (identical inlet velocity and fluid properties) that reflect common microfluidic use were compared. Consequently, all pressure, velocity, and WSS outcomes are interpreted as device-scale responses under fixed velocity, rather than a fundamental isolation of cross-section shape, which would require matched hydraulic diameters or flow rates. This study is explicitly device-oriented, representing a fixed operating point rather than a strict geometric isolation. Accordingly, the results are also expressed with dimensionless loss coefficients (Ktot and Klocal) to enable scale-independent, device-level comparison. Full article

Aortic valve calcification is the process of calcium buildup on the leaflets of the aortic valve, preceding functional insufficiency. Calcification underlies the development of aortic stenosis by stiffening the valve leaflets, leading to restricted aortic valve opening during systole and obstructed blood flow. However, a more comprehensive understanding of the hemodynamic effects of altered valve properties is required. Therefore, it is crucial to investigate the biomechanical properties of aortic valve leaflets susceptible to calcification. To examine fluid flow in an aorta segment with leaflets of different stiffness, a two-way fluid–structure interaction model was developed. The leaflet’s behavior was modeled using two constitutive laws—linear-elastic and isotropic hyperelastic—followed by numerical testing and comparative analysis. Using the material parameter values $c_{01}$ and $c_{10}$ within the ranges of 22–60 and 22–60 kPa, respectively, the hyperelastic model was examined. The valve leaflets’ Young’s modulus ranged from 1 to 22 MPa, while their Poisson’s ratio ranged from 0.35 to 0.45. A high correlation between Poisson’s ratio and wall shear stress was found. With an elastic modulus of 22 MPa and the highest Poisson’s ratio of 0.45, the maximum wall shear stress was 81.78 Pa during peak flow velocity and complete valve opening, while the lowest wall shear stress was 0.38 Pa. We can infer from the study’s results that, when considering the isotropic structure and nonlinear characteristics of valve leaflets, the Delfino hyperelastic model more accurately depicts their complex behavior. Full article

Permeability evolution in remolded fault gouge creates critical uncertainties in geotechnical parameterization for dam foundations. However, the underlying multi-scale mechanisms, including mineral migration and pore structure changes, remain insufficiently understood. This study investigates these mechanisms using remolded plastic-thrust fault gouge from the Yulong Kashi hydropower project in China. We developed an innovative sample preparation method that combines in situ mineral self-cementation and directional compaction. The study integrated multidisciplinary tests including field in situ permeability tests; seepage–stress coupling tests; and micro-scale NMR/XRD/SEM-EDS analyses. Results demonstrate that remolded samples exhibit 1–2 orders of magnitude lower permeability (10⁻⁷ cm/s) than in situ samples (10⁻⁵ cm/s). This significant reduction is primarily caused by the loss of cementing agents and the uniform compaction of remolded samples, which leads to degraded pore connectivity. SEM-EDS analysis highlighted the leaching of cementing materials (such as K⁺, Ca²⁺ ions), while XRD revealed changes in mineral composition, with chlorite dissolution being the primary mineral alteration associated with permeability decay. Additionally, artificially enhanced cohesion distorted the mechanical behavior of the samples. These findings provide an explanation for why conventional laboratory tests tend to underestimate in situ permeability and overestimate shear strength in fault zones. This study establishes microstructure-informed correction frameworks for hydraulic and mechanical parameters in fault-crossing hydraulic engineering applications Full article

China’s loess deposits exhibit high vulnerability to deformation under precipitation and snowmelt, posing significant risks to infrastructure. This study utilized enzyme-induced carbonate precipitation (EICP) to enhance the mechanical properties of Yili loess. Comparative analyses of untreated and EICP-treated samples were conducted using unconfined compression strength (UCS) tests, unconsolidated–undrained (UU) triaxial shear tests, and scanning electron microscopy (SEM). Results demonstrated that urease activity increased markedly between 25–65 °C, while calcium carbonate production peaked at 55 °C before declining. EICP treatment elevated UCS by 52% relative to untreated soil and altered the failure mechanisms: untreated specimens failed through penetrating shear cracks, whereas treated specimens exhibited compressive failure with vertical fissures. Triaxial tests confirmed enhanced properties in EICP-stabilized loess, showing 8.3–10.7% higher failure strength and 15.7% greater cohesion (increasing from 31.3 kPa to 36.2 kPa), while the internal friction angle remained largely unchanged. Microstructural analysis revealed that EICP generated continuous cementitious layers and crystal bridges of vaterite, transforming particle contacts from point-to-point to surface-to-surface interfaces. Simultaneously, crystal precipitation reduced pore sizes and increased tortuosity. These micro-scale modifications improved interparticle friction constraints and stress transfer efficiency, thereby enhancing the macroscopic mechanical performance. The findings validate EICP’s efficacy for stabilizing collapsible loess deposits and provide insights for geohazard mitigation in similar engineering contexts. Full article

Mitigating wake effects between wind turbines is crucial for enhancing the overall output power of offshore wind farms. Therefore, optimizing turbine spacing and layout under turbulent conditions is essential. This study employs the NREL-5 MW wind turbine model to investigate the efficiency of a 3 × 3 wind farm. This research focuses on the influence of turbine spacing and layout on wake field distribution and output power characteristics under different turbulence intensities. A key innovation is the application of entropy production theory to quantify energy dissipation and wake recovery, providing a deeper understanding of the underlying mechanisms in energy losses. This research also introduces fatigue analysis based on the Damage Equivalent Load (DEL) method, revealing that staggered layouts significantly reduce cyclic loads and extend turbine lifespan. The results indicate that modifying the layout is a more effective strategy for enhancing the total power output of the wind farm, which proves to be more effective than altering the turbulence intensity. Specifically, staggered layout I (with a downstream stagger of 1.0 rotor diameter (D)) increases total output power by 28.76% (to 36.84 MW) and causes a 16.38% surge in power when the spacing increases to 5D. Expanding the wind turbine spacing mitigates wake interaction, resulting in a dramatic 59.84% power recovery for downstream wind turbines. The wind turbine’s lifespan is extended as a result of fatigue loads on the root bending moment being substantially reduced by the staggered layout, which alters the wake structure and stress distribution. The entropy production analysis shows that regions with high entropy production are primarily concentrated near the rotor and within the wake shear layer. The energy dissipation is substantially reduced in the case of staggered layout. These findings provide valuable guidance for the aerodynamic optimization and long-term operation design of large-scale wind farms, contributing to improved energy efficiency and reduced maintenance costs. Full article

Uniaxial compression testing provides essential mechanical property characterization for intact rock specimens. The accuracy of specimen preparation critically affects compression test results through end-surface geometry deviations: parallelism, perpendicularity, and diameter tolerance. Specimen end-surface parallelism is affected by surface irregularities (e.g., protrusions, warping), whereas perpendicularity deviations indicate angular misalignment of the specimen with the loading axis. This study develops a 3D uniaxial compression model using RFPA3D, with rigid loading plates to simulate realistic boundary conditions. Three typical end-surface defects are modeled: protrusions (central/eccentric), grooves, and unilateral warping. Specimens with varying tilt angles are generated to evaluate perpendicularity deviations. Simulation results reveal that central end-surface protrusions induce: (1) localized stress concentration, which forms a dense core, and (2) pronounced wedging failure when protrusion height exceeds critical thresholds. Eccentric protrusions trigger characteristic shear failure modes, while unilateral warping causes localized failure through stress concentration at the deformed region. Importantly, end-surface grooves substantially alter stress distributions, generating bilateral stress concentration zones when groove width exceeds critical dimensions. Full article

Osteocytes translate fluid shear stress into biochemical signals critical for bone homeostasis. Here, we combined 3-dimensional (3D) osteocyte culture, microgravity simulation, fluid shear mimicking reloading after disuse, and real-time calcium signaling analysis to elucidate responses of osteocytes under different mechanical environments. Ocy454 cells were seeded onto 3D scaffolds and cultured under static (control) or simulated microgravity (disuse) conditions using a rotating wall vessel bioreactor. Elevated expression levels of Sost, Tnfsf11 (Rankl), and Dkk1 were detected following disuse, confirming efficacy of the microgravity model. Cell membrane integrity under mechanical challenge was evaluated by subjecting scaffold cultures to fluid shear in medium containing FITC-conjugated dextran (10 kDa). The proportion of dextran-retaining cells, indicative of transient membrane disruption and subsequent repair, was higher in microgravity-exposed osteocytes than controls, suggesting increased susceptibility to membrane damage upon reloading following disuse. Intracellular calcium signaling was assessed under a high but physiological fluid shear stress (30 dynes/cm²). Scaffolds cultured under disuse conditions demonstrated a larger sub-population of osteocytes with high calcium signaling intensity (F/Fo > 10 fold) during fluid shear. The maximum fold change in calcium signaling intensity over baseline and the duration of the peak calcium wave were greater for osteocytes cultured under disuse as compared to static controls, however the bioreactor-cultured osteocytes showed, on average, fewer calcium waves than those cultured under control conditions. Subsequent experiments demonstrated that the sub-population of osteocytes with high calcium signaling intensity following exposure to disuse were those that had experienced a transient membrane disruption event during reloading. Together, these results suggest that simulated microgravity enhances osteocyte susceptibility to formation of transient membrane damage and alters intracellular calcium signaling responses upon reloading. This integrated approach establishes a novel platform for mechanistic studies of osteocyte biology and could inform therapeutic strategies targeting skeletal disorders related to altered mechanical loading. Full article

Preeclampsia (PE) is a major cause of maternal and perinatal morbidity, and early prediction is critical for timely intervention. This study aimed to identify predictive biomarkers for PE through transcriptomic analysis of second-trimester amniotic fluid supernatant (AFS) collected prior to clinical symptom onset. AFS samples from women who later developed PE (n = 7) and matched controls (n = 7) underwent RNA sequencing to identify differentially expressed genes (DEGs). Candidate genes were validated by real-time PCR in HTR-8/SVneo cells exposed to fluid shear stress at 3, 10, and 20 dyn/cm² for 24 h, mimicking the hemodynamic environment of PE, and siRNA-mediated knockdown was used to assess effects on trophoblast migration and invasion. RNA sequencing revealed 19 DEGs, with 3 upregulated and 16 downregulated genes in the PE group. HOOK2 emerged as the most significantly upregulated gene. Four candidate genes, including HOOK2, CCDC160, CKB, and PARP15, were selected for further validation. HOOK2 mRNA expression significantly increased with higher shear stress levels, consistent with sequencing data. Knockdown of HOOK2 led to a significant increase in trophoblast invasion, while migration showed no significant change. These findings suggest that HOOK2 may serve as a promising early biomarker for PE by modulating trophoblast invasiveness under altered hemodynamic conditions, with potential to improve risk stratification and personalized monitoring in pregnancy. Full article

Canals have played a significant role in promoting the prosperity of the shipping industry worldwide. Meanwhile, canal construction can alter the hydro-morphodynamic processes in coastal tidal channels. The Fangchenggang Canal is an extension route of the Pinglu Canal, which connects southwestern regions to the Beibu Gulf in the South China Sea by cutting across approximately 20 km of intertidal and dry land of the Qisha peninsula. A two-dimensional numerical model based on MIKE21 has been established to investigate the variations of tidal current structures and sediment transport characteristics. The maximum flow velocity within the main channel increases up to 1.18 m/s in the marine section. A bidirectional flow pattern has been observed in the land excavation segment. Numerical simulations of the sedimentation processes demonstrated potential erosion in the land excavation section due to the increased bed shear stress. The present study shares useful insights into the response mechanism of hydro-morphodynamic processes under canal construction. The quantitative simulations would support the environmental assessment and route planning of canal projects. Full article

Caspases are known for their roles in cell death and inflammation. However, emerging evidence suggests they also mediate non-lethal processes, governed by a finely tuned balance of localization, activity, kinetics, and substrate availability. Given that many caspase substrates are implicated in mechanoadaptive processes, we investigated if caspases contribute to morphological adaptation of human pulmonary artery endothelial cells to fluid shear stress and other morphology-altering stimuli in vitro. Using selective inhibitors, we screened all major caspases for a role in endothelial cell adaptation to unidirectional laminar shear stress (15 dyn/cm², 72 h). Selective inhibition of caspase-6, but not other caspases, impaired morphological shear adaptation. Only 5.5% of caspase-6-inhibited cells shear-adapted vs. 75.2% of vector controls. Live-cell FRET imaging revealed progressive caspase-6 activation starting at 18 h of shear stress, coinciding with the onset of morphological remodeling. The active caspase-6 localized predominantly perinuclearly, while caspase-3 remained inactive throughout shear exposure. Caspase-6 inhibition did not affect elongation in response to alternative biomechanical or biochemical stimuli, including uniaxial cyclic stretch (5%, 1 Hz), spatial confinement on narrow micropatterned RGD-lines, or TNF-α stimulation, nor did it impair cell adhesion, directed migration, wound healing, or barrier recovery after wounding. Our study uncovers a previously unidentified role of caspase-6 as a non-apoptotic, mechanosensitive effector specifically required for shear-induced morphological adaptation of pulmonary artery endothelial cells, highlighting a novel regulatory axis in vascular mechanoadaptation. Full article