Search Results (93)

Iris sanguinea features upright, stiff leaves, making it an excellent cut-foliage material, with its tall leaf architecture greatly enhancing ornamental value in landscaping. However, during the leaf expansion phase, plants frequently exhibit loose foliage arrangement, excessive spreading, and compromised mechanical strength, culminating in lodging and a concomitant decline in ornamental quality. Plant height in I. sanguinea is strongly regulated by phytohormones. This study showed that exogenous GA at concentrations of 50 mg·L⁻¹, 100 mg·L⁻¹, and 200 mg·L⁻¹ increased seedling height by 5.7%, 8.8%, and 12.7%, respectively, through foliar spraying on I. sanguinea seedlings grown ex vitro in a greenhouse; conversely, PAC treatment at equivalent concentrations suppressed growth by 19.3%, 21.0%, and 22.2%, respectively. Two pivotal GA signaling components, GAI and GID1a, were isolated from I. sanguinea. Subcellular localization confirmed that both IsGAI and IsGID1a proteins localize to the nucleus. Overexpression vectors pCAMBIA1300-IsGAI-GFP and pCAMBIA1300-IsGID1a-GFP were constructed and expressed in Arabidopsis thaliana. Transgenic lines overexpressing IsGAI showed significantly reduced plant height, hypocotyl elongation, and bolting, whereas IsGID1a overexpression promoted these traits. Exogenous GA application partially reversed the dwarf phenotype induced by IsGAI overexpression and further potentiated the height enhancement observed in IsGID1a-overexpressing lines. This study identifies two key genes controlling plant height and provides a theoretical basis and genetic resources for precisely engineering plant architecture in I. sanguinea. This is especially important for developing dwarf varieties with enhanced ornamental and agronomic traits, offering significant potential in the landscaping and cut flower industries. Full article

Background/Objectives: With the acceleration of global industrialization and continuous population growth, the world is increasingly confronted with the dual challenges of food insecurity and cultivated land contamination. The screening and breeding of rice varieties with superior agronomic traits and low heavy metal accumulation have therefore become important strategies for ensuring food safety and sustainable agricultural production. Methods: In this study, rice varieties carrying the Gn1a-i gene and exhibiting specific cadmium (Cd) accumulation characteristics were screened using a combination of molecular marker detection and cadmium accumulation evaluation. Specific loop-mediated isothermal amplification (LAMP) primers targeting the Gn1a-i gene were designed and combined with a lateral flow dipstick (LFD) assay to enable rapid genetic screening of rice varieties. A six-day hydroponic experiment under cadmium stress was conducted across three temperature ranges (15–20 °C, 22–27 °C, and 30–35 °C), and cadmium accumulation in different plant organs (roots, stem sheath, and leaves) was analyzed. Results: Seven varieties carrying the Gn1a-i gene, including Xiangwanxian 12, were identified among ten tested rice varieties. Xiangwanxian 12 was subsequently selected for further evaluation, with the high-cadmium-accumulating variety Yuzhenxiang used as a control. At 144 h, the total Cd content in the measured organs of Xiangwanxian 12 was 9.6%, 4.0%, and 23.2% lower than that of Yuzhenxiang under low, medium, and high temperatures, respectively (one-tailed t-test, p < 0.01 for all three temperatures). Conclusions: The integration of LAMP-based genotyping and physiological evaluation provides a novel and reliable strategy for identifying low-Cd rice germplasm. Xiangwanxian 12, which carries the Gn1a-i allele and exhibits consistently lower Cd accumulation than Yuzhenxiang, suggests potential as a candidate for breeding high-yield, low-Cd rice cultivars. Full article

Phytoremediation is increasingly recognized as a sustainable and low-impact approach for the remediation of contaminated and marginal soils, particularly when combined with the cultivation of resilient non-food crops. Castor bean (Ricinus communis L.) is a multipurpose industrial oilseed crop characterized by high biomass production, strong tolerance to abiotic stresses, and a remarkable ability to accumulate and tolerate potentially toxic elements. This review provides a comprehensive overview of the role of castor bean in phytoremediation systems, integrating agronomic management, physiological traits, traditional and industrial uses, and sustainability perspectives. Particular attention is given to agronomic practices that enhance plant establishment and remediation efficiency on contaminated lands. Beyond its environmental role, this review highlights the long-standing traditional uses of castor oil and the growing importance of castor bean as an energy and industrial crop, supplying renewable feedstocks for biofuels, bio-based chemicals, and materials within a circular economy framework. While genetic improvement and molecular tools offer future opportunities to optimize specific traits, the current potential of castor bean relies largely on its agronomic adaptability and multifunctionality. Overall, R. communis emerges as a strategic species for integrated phytoremediation systems that couple soil restoration with renewable resource production and sustainable land management. Full article

Although greenhouse vegetable production is rapidly shifting toward innovative soilless systems, soil-based conventional cultivation still dominates globally. This production system faces growing pressure to transition to sustainable practices. However, introducing biofertilisers into intensive systems often yields inconsistent results. Specifically, their effects on different lettuce traits vary due to complex relationships between genotype, biofertiliser, environmental conditions, and market demands. Single-parameter evaluations fail to balance conflicting criteria, necessitating multi-criteria decision-making (MCDM) methods for selecting optimal choices. This study aims to overcome these inconsistencies through an integrated fuzzy MCDM-based optimisation model. Three lettuce cultivars (‘Carmesi’, ‘Aquino’, and ‘Gaugin’) were grown in an unheated Surčin (Serbia) greenhouse during a 58-day autumn experiment using a complete block design. Four treatments were applied: a control (without fertilisation), effective microorganisms, a Trichoderma-based fertiliser, and their combination. Biofertilisers were applied before transplanting and four times foliarly during the vegetation period via battery sprayer. This defined 12 production models (cultivar–fertiliser pairs), evaluated across 10 criteria: agronomic (core ratio, number of leaves), quality (nitrate content, total antioxidant capacity, total soluble solids, and chlorogenic acid), sensory (overall taste, overall quality), and economic (total variable costs, total income). Four decision-making experts from the Faculty of Agriculture and the ready-to-eat salad industry assessed weighting coefficients using the fuzzy PIPRECIA (PIvot Pairwise RElative Criteria Importance Assessment) method. The fuzzy MARCOS (Measurement Alternatives and Ranking according to COmpromise Solution) method was used to rank the alternatives. To confirm the stability of the obtained ranking with the fuzzy MARCOS method, we performed sensitivity analysis through 20 different scenarios. Applied fuzzy methods identified alternative A11—‘Aquino’ cultivar with combined biofertilisers—as the best-ranked option, followed by A6 and A7. This study validates fuzzy PIPRECIA and fuzzy MARCOS as effective tools for optimising lettuce production models. They support farmers in selecting the most favourable solution based on multiple criteria, aiding the shift from mineral fertilisers to sustainable biofertiliser-based systems in intensive production—especially helpful for producers making this transition. Full article

This study provides an integrated agronomic–functional–nutritional–bioactive characterization of three Oxalis tuberosa varieties (Yellow, Pink, and Black) cultivated under open-field conditions. Unlike previous studies that have typically examined isolated trait groups or single quality dimensions, this work simultaneously evaluates yield-related morphology, starch functional behavior, proximate composition, antioxidant activity, and pigment-associated color attributes within a unified experimental framework, enabling robust varietal comparison and application-oriented interpretation. The Yellow variety matured later (125 ± 2 days) and produced the highest total biomass (587 ± 32 g) and yield per plant (462 ± 28 g), with the longest tubers (8.7 ± 0.3 cm) and the greatest tuber number (12.1 ± 1.1 per plant). Functional assessments indicated that the Yellow variety exhibited superior swelling capacity (10.2 g/g) and solubility index (6.3%), together with the highest starch content (68.4 ± 2.1 g/100 g DW). Nutritional profiling further showed lower moisture and higher carbohydrate levels in the Yellow variety compared with the other varieties, supporting its suitability for food processing and agricultural production. In contrast, the Black variety showed the strongest antioxidant potential, with higher DPPH scavenging activity (46.2 ± 1.3%), total phenolics (5.9 ± 0.3 mg GAE/g DW), and flavonoids (2.3 ± 0.1 mg QE/g DW), consistent with its darker pigmentation and greater nutraceutical potential. The novelty of this study lies in its integrated, multi-trait comparison of oca varieties under the same open-field conditions with standardized agronomic management, allowing for the first simultaneous assessment of agronomic performance, starch functionality, nutritional quality, antioxidant capacity, and color attributes. Overall, these findings highlight the importance of varietal selection in determining agronomic performance, starch functionality, nutritional composition, and bioactive traits in Oxalis tuberosa, providing actionable evidence for targeted agricultural and industrial applications. Full article

Auricularia cornea is rich in nutrients and bioactive compounds, and with the continuous expansion of its industrial cultivation, elucidating the genetic basis of key agronomic traits is essential for marker-assisted breeding and cultivar improvement. In this study, 140 A. cornea germplasm accessions representing diverse geographic origins were subjected to a comprehensive two-year phenotypic evaluation of ten agronomic traits, including FBL, FBW, FBT, Yield, MGR, GP, and fruiting body color. The results showed that all traits exhibited substantial genetic variation across the population, and most traits displayed high heritability. Genome-wide association studies (GWAS) identified 1178 and 821 SNPs significantly associated with agronomic traits in the two respective years, with yield-related loci predominantly located on chromosome 9. Based on an integrated significance threshold, ten core SNPs were ultimately retained, and 42 putative candidate genes were identified within ±5kb flanking regions of these SNPs. These candidate genes were mainly involved in cell wall polysaccharide modification, redox regulation, pigment biosynthesis, metabolic processes, and signal transduction. Furthermore, haplotype analysis identified six superior haplotypes associated with ear morphology, yield, MGR, and GP, and accessions carrying these superior haplotypes exhibited significantly enhanced phenotypic performance. Overall, this study provides a systematic dissection of the genetic architecture of important agronomic traits in A. cornea and offers a solid theoretical foundation for high-yield and high-quality molecular breeding and genetic improvement. Full article

Cotton (Gossypium hirsutum) is globally cultivated for its high-quality fiber; yet, its seed, rich in oil and protein, offers untapped potential for various applications, including food, feed, and industry. With cottonseed oil gaining renewed attention as a valuable co-product, efforts to enhance oil content must contend with long-standing breeding priorities focused on lint yield and fiber quality. A central challenge lies in the complex and often antagonistic genetic relationships between oil accumulation and key agronomic traits. Notably, negative correlations between seed oil content and fiber yield, as well as the pleiotropic nature of several regulatory genes and Quantitative Trait Loci (QTLs), present significant barriers to dual-trait improvement. This review synthesizes current knowledge on the genetic and molecular interplay between cottonseed oil content and other agronomic traits. We examine the architecture of oil-related QTLs and pleiotropic loci, co-expression patterns of shared transcriptional regulators, and metabolic trade-offs influencing carbon allocation between seed and fiber. Recent advances in genomics, transcriptomics, and systems biology are explored as tools to disentangle these trait interactions. We highlight strategies such as multi-trait genomic selection, CRISPR-based uncoupling of antagonistic loci, and the use of wild and exotic germplasm to overcome linkage drag. By providing an integrative overview of the constraints and opportunities at the intersection of oil and agronomic trait improvement, this review lays the groundwork for the development of dual-purpose cotton ideotypes. We propose a conceptual framework for breeding programs to simultaneously enhance fiber yield and oil productivity in a sustainable and climate-resilient manner. Full article

Cotton is one of the most significant economic crops cultivated worldwide. Cottonseed is a strategic reservoir of high-quality plant protein and an underexploited resource for the food and feed industries. To quantify nutritional diversity and identify superior germplasm, we evaluated 312 upland cotton (Gossypium hirsutum L.) accessions over two consecutive growing seasons and characterized 30 agronomic and nutritional traits. Protein content varied widely (29.6–48.8%), with a coefficient of variation of 7.5–11.7% and a two-year mean of 37.0%. Glutamic acid (Glu; 154.0 mg/g) and aspartic acid (Asp; 90.7 mg/g) were the most abundant amino acids, and lysine and arginine were relatively high among essential amino acids. Correlation analysis based on genotype best linear unbiased estimates (BLUEs) showed that most nutritional traits were positively or neutrally associated with key yield-related traits, particularly lint percentage (LP) (e.g., protein vs. LP: r = 0.18, p < 0.01), indicating the feasibility of simultaneous improvement in seed nutritional quality and lint yield potential. Using 29 core traits with complete two-year data, we developed an integrated evaluation framework combining principal component analysis (PCA), grey relational analysis (GRA), TOPSIS, and the analytic hierarchy process (AHP) to rank accessions comprehensively. This framework identified 10 elite germplasm lines with high protein content and favorable yield potential, exemplified by “Xinluzhong 34” (Rank 1; phenotypic comprehensive value, P_i = 0.733). These results provide a quantitative foundation for value-added cottonseed utilization and support breeding strategies aimed at developing cultivars with both high yield and enhanced nutritional quality. Full article

To address the “fungus-forest conflict” in the edible mushroom industry and the challenge of resource utilization for bamboo substrate waste, this study focused on white Auricularia cornea, and cultivation systems were established with bamboo substrate replacing wood chips at ratios of 0%, 18%, 38%, 58%, and 78%. By integrating liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis with agronomic trait measurements, the study elucidated the metabolic adaptation mechanisms of the substrate. Results indicated that white A. cornea could grow normally in all bamboo substrates, with the 58% bamboo substrate replacement group (D_58) demonstrating the most optimal overall performance. The mycelial growth rate reached 3.55 ± 0.24 mm/d, and the growth period was the shortest (86.2 d), balancing growth efficiency with cost advantages. Metabolomics detected 3779 metabolites, primarily amino acids and their derivatives (42.2%) and organic acids (35.54%). Compared to the control group, each treatment group exhibited 104–528 upregulated and 192–630 downregulated differential metabolites, with 93 shared differential metabolites and numerous unique markers. KEGG pathway enrichment analysis revealed that varying bamboo substrate ratios shaped growth adaptation strategies by regulating core pathways such as nucleotide metabolism and ABC transporters. This study established the feasibility and optimal formulation of bamboo substrate substitution, elucidated the substrate–metabolite–phenotype linkage mechanism, and provided theoretical foundations and practical references for high-quality cultivation of white A. cornea and sustainable development through “substituting bamboo for wood” to reduce carbon emissions. Full article

To reduce the production cost of Pleurotus giganteus and to valorize agricultural waste, this study investigated the effects of substituting cottonseed hull with corn cob (a major lignocellulosic by-product of maize production) on the mycelial growth, agronomic traits, nutrient composition, commercial quality, and economic benefits of P. giganteus ‘Shenxun No.1’. The aim was to verify the feasibility of this substitution and screen optimal substrate formulations for industrial cultivation. Four substrate formulations with corn cob substitution ratios of 0% (T1), 15% (T2, control), 30% (T3), and 45% (T4) were designed, while adjusting cottonseed hull proportions to 45%, 30%, 15%, and 0%, respectively. Mycelial colonization performance, fruiting body agronomic traits (yield and cap/stipe characteristics), nutrient contents (crude protein, crude fiber, etc.), and commercial traits (marketable yield and production cost) were systematically determined and analyzed. The results showed that corn cob content exceeding 15% prolonged the substrate bag colonization time by 5–7 days, but T4 (45% corn cob) resulted in the densest mycelia with excellent structural development. In terms of fruiting bodies, T4 exhibited the highest yield in the second harvest flush and the highest total yield across three flushes. Nutritionally, crude protein content of fruiting bodies decreased by 10.48% in T4 compared to T1, while crude fiber content increased with rising corn cob proportion; no significant difference in crude polysaccharide content was observed among formulations. Importantly, corn cob substitution did not impair the commercial traits of fruiting bodies, and T4 achieved the lowest material cost per bag (0.78 CNY) with an optimal cost–benefit ratio. In conclusion, corn cob is a viable and cost-effective substitute for cottonseed hull in P. giganteus cultivation, and the 45% substitution formulation (T4) is recommended for industrial production due to its superior yield performance and economic benefits. This study provides a theoretical basis for sustainable utilization of agricultural waste and optimization of P. giganteus cultivation systems. Full article

Rapeseed (Brassica napus L.) is a major oilseed crop worldwide, particularly valued for its high yield potential, favorable fatty acid composition, and its bioactive compounds that improve nutritional and industrial quality. However, its productivity and oil quality are increasingly compromised by climate change-induced water scarcity, particularly in semi-arid Mediterranean regions. In this study, the performance of 17 genotypes was evaluated under well-watered (irrigated) and rainfed (stressful) conditions across two contrasting locations, Douyet Experimental Station (DYT) and Ecole Nationale d’Agriculture de Meknès (ENAM), during the 2023/2024 growing season. The assessment concerned seed yield, oil traits, and nutraceutical quality. The results obtained show that drought stress significantly reduced seed yield by about 26% (from 2.29 to 1.69 t ha⁻¹) and decreased oil content by about 8.3% (from 41.1% to 37.7%). The highest reductions were observed for sensitive genotypes, particularly ‘IND23’, ‘IND82’, and ‘H2M-5’, while ‘Moufida’, ‘Nap9’, and ‘TP2’ maintained seed and oil yield above the overall average across both water regimes. Drought also impacted the accumulation of secondary metabolites, with mean total phenolic content increasing from 5.41 to 9.98 mg GAE g⁻¹ (+84.5%) and total flavonoid content rising from 25.25 to 34.93 mg QE g⁻¹ (+38.3%) under rainfed conditions, accompanied by marked increases in antioxidant activity (DPPH, ABTS), particularly for ‘Moufida’, ‘Nap9’, ‘TP2’, and ‘Marina’. Oil quality indices remained within Codex Alimentarius standards, with a slight increase in acidity values accompanied by a decrease in peroxide values, thus suggesting improved oxidative stability. Principal component analysis grouped genotypes into distinct clusters, with an elite group (‘Moufida’, ‘Nap9’, and ‘TP2’) characterized by yield stability, accumulation of phenolics, and high antioxidant activity, thus confirming their strong adaptation to the dry conditions of the Mediterranean region. These drought-tolerant lines, with high agronomic performance combined with good nutritional quality, can be recommended as valuable cultivars for sustainable and high-quality rapeseed production in dry Mediterranean regions. Full article

Anise (Pimpinella anisum L.) is one of the most important annual herbs of the Apiaceae family, widely cultivated in southern Spain. Their seeds are highly valued for culinary uses and for producing quality essential oils widely used in food and beverage products, as well as for industry, medicinal, and cosmetics applications. This study investigates the seed yield and essential oil content within a set of 50 anise accessions from worldwide origin, as well as their composition by GC–MS and GC–FID analysis. Accessions showed significant differences in the agronomic parameters measured, including plant height (cm), seed yield (kg ha⁻¹), and the Harvest Index (%), with accessions PA_87 (Spain), PA_47 (Greece), and PA_21 (unknown origin) being the most performant. Essential oil (EO) content varied between 0.8% and 5.7% across different genotypes, resulting in EO production values ranging from 0.1 to 300 kg ha⁻¹. Trans-anethole was identified as the dominant terpene, comprising 84.4% to 94.4% of the content, followed by eugenol (1.4% to 5.5%) and α-muurolene (1.4% to 7.2%). PCA analysis identified five distinct groups and one outlier, influenced by minor terpenes. Indeed, there was a strong negative correlation between estragole and pseudoisoeugenyl 2-methylbutyrate. This study underscores the significance of minor terpenes, which play crucial roles in defining unique aniseed chemotypes, allowing for the selection of cultivars optimized for specific uses in food, cosmetics, and pharmaceuticals. Additionally, these findings emphasize the impact of cultivar genetics on agronomic traits and EO profiles, suggesting the need for further research to optimize plant growth and yield and EO quality. Full article

Light is a crucial environmental regulator for Tricholoma giganteum (T. giganteum). This study investigated the effects of light quality and photoperiod on its growth, physiology, and nutritional composition. During the mycelial stage, blue light (BL) exposure for 5 d promoted the highest growth rate (0.74 mm d⁻¹, 45% higher than dark control, p < 0.05). Red light (RL) enhanced antioxidant capacity, elevating superoxide dismutase (SOD) activity to 240.20 U·mL⁻¹ (after 5 d) and DPPH radical-scavenging activity to 276.11% (after 3 d). Ultraviolet (UV) suppressed polyphenol oxidase (PPO) activity. BL also increased mycelial polysaccharide content (6.45 g·100 g⁻¹). In the fruiting stage, green light (GL) improved agronomic traits and first-grade yield (3.75 kg), while also promoting the accumulation of glutamate (4.39 g·100 g⁻¹), a key umami compound. Further photoperiod optimization revealed that 4 h of daily GL exposure shortened the fruiting cycle, achieved the highest biological efficiency (98.4%), and maximized both polysaccharide (38.17 g·100 g⁻¹) and glutamate contents (5.70 g·100 g⁻¹). These results recommend a two-stage lighting protocol: BL for mycelial growth and a 4 h daily GL for fruiting, providing a scientific basis for the industrial cultivation of T. giganteum. Full article

Medium-chain fatty acids (MCFAs), with carbon chain length of 6 to 12 carbon atoms, have received substantial attention in metabolism and applications in health, agriculture and industry. They have shown promising therapeutic effects for metabolic disorders such as obesity, diabetes, and neuro-triglycerides (MTGs) enriched in fatty acids. Rice (Oryza sativa L.) is one of the most widely used starchy crops around the world. New varieties have a lower level of starch and include lipid profiles that could make rice oil possess better nutritional quality and generate novel possibilities in biofuel production. With the help of new genetic and breeding techniques, the expression of genes that involve fatty acid biosynthesis can be altered. CRISPR/Cas9 and marker-assisted selection (MAS) are currently enhancing the fatty acid content in rice varieties without affecting the yield and various agronomic traits. This review presents the progress on the knowledge of rice MCFA biosynthetic pathways, genetic determinants of MCFA biosynthesis, and breeding technologies for enhancing MCFA production in rice. It also addresses wider implications of this research, including possible enhancement in human nutritional quality and development of sustainable agriculture. Full article

A multi-factor analysis of cultivar, biofertiliser, and growing season was conducted to optimise lettuce agronomic and quality traits in diverse soil conditions. The goal was to identify soil differences and offer practical recommendations to improve lettuce traits and quality for farmers and the processing industry. The study employed a complete block design with four treatments, three involving biofertilisers, applied to six lettuce cultivars grown in two contrasting soil types- Mollic Gleysol (Calcaric)-GL and Hortic Anthrosol (Terric, Transportic)-AT, across three consecutive greenhouse seasons (autumn, winter, and spring). Biofertilisers were applied to the soil before transplanting and foliarly during the growing cycle, with four of the following treatments: control (no fertilisation), a fertiliser containing beneficial microorganisms, a Trichoderma-based fertiliser, and a combination of both. In GL soil, all biofertiliser treatments increased rosette height, leaf number, and stem length, whereas in AT soil, all morphological parameters declined significantly. The green cultivars ‘Aquino’ and ‘Kiribati’ showed superior morphological performance, particularly in spring and winter. Rosette fresh weight, a key indicator of plant biomass, reached 236.4 g in ‘Aquino’ grown in GL soil, and 208.6 g in ‘Kiribati’ grown in AT soil. Dualex™ leaf sensor measurement indicated that ‘Aquino’ exhibited the highest nitrogen balance index (NBI), while the red cultivar ‘Gaugin’ recorded the highest chlorophyll, flavonoid, and anthocyanin contents. Combined fertilisers increased NBI by 6.3% and chlorophyll by 6.8% in GL soil. Trichoderma fertiliser alone raised NBI by 6.8% in GL soil, whereas in AT soil, plants accumulated more flavonoids and anthocyanins (by 9.2% and 8.5%). Optical parameters were highest in autumn. The three-factor experiment demonstrated that cultivar, biofertiliser, and growing season significantly influenced the majority of measured traits. Correlation analysis revealed that rosette fresh weight was positively associated with NBI but negatively correlated with quality-related traits. Based on these findings, cultivars ‘Aquino’, ‘Kiribati’, and ‘Gaugin’ are recommended for both farmers and the processing industry to improve lettuce production quantity and quality. Overall, cultivar, biofertiliser, and season strongly influenced the measured parameters, underscoring the importance of tailoring biofertiliser application to soil type and season to achieve optimal production outcomes. Full article