Search Results (429)

Background/Objectives: Emerging evidence suggests that long non-coding RNA (lncRNA) molecules influence the adaptive response to exercise, but how lncRNA responses differ between endurance and resistance exercise (RE) modalities is poorly understood. The purpose of this study was to bioinformatically infer the expression of lncRNA in skeletal muscle following acute aerobic exercise (AE) and RE. Methods: We downloaded publicly available RNA-seq data, performed a differential expression (DE) analysis, and compared lncRNA expression profiles between different exercise types (AE vs. RE) at three timepoints: baseline, 1 h post-exercise, and 4 h post-exercise. Results: We observed distinct lncRNA profiles between acute AE and RE at different timepoints, suggesting that lncRNA perform distinct roles in controlling the response to different exercise modalities in skeletal muscle. Conclusions: Future studies should investigate the specific roles of these lncRNAs in the response to acute exercise in skeletal muscle. Full article

Understanding the long-term effectiveness of warm-up strategies is essential for enhancing neuromuscular performance in youth soccer players. This study examined the long-term effects of integrating squat exercises into the final phase of the warm-up over nine weeks on sprint, jump, change-of-direction (COD), and aerobic performance in youth soccer players. Twenty-four male U17 players were randomly assigned to either a squat-based warm-up (experimental group [EG]) or a rondo-based warm-up (control group [CG]). The EG trained twice weekly using 3–4 sets of 4–12 repetitions at progressively increasing intensities (50–85% of 1-RM). Performance was assessed pre- and post-intervention using 10 and 30 m sprint, squat jump (SJ), countermovement (CMJ), standing long jump (SLJ), 5-jump (5JT), T-half (COD), and VAMEVAL tests. The EG showed small to large significant gains in sprint (10 m: −2.21%, Cohen’s d [d] = 1; 30 m: −1.6%, d = 0.58), jumping (SJ: +9.29%, d = 1.23; CMJ: +12.08%, d = 1.83; SLJ: +7.14%, d = 0.8; 5JT: +2.33%, d = 0.32), and COD (−1.41%, d = 0.32), while aerobic endurance showed no significant change (p > 0.05). The CG showed no significant improvements (p > 0.05). Overall, integrating brief, progressive squat exercises at the end of warm-ups twice weekly led to chronic improvements in explosive neuromuscular performance, with minimal impact on aerobic endurance. Full article

Aim: To investigate the effect of a 6-week sport-specific BET intervention on cognitive and physical performance in elite orienteering athletes. Methods: A single-arm cross-over study with an initial 6-week control period (CON) followed by a 6-week brain endurance training (BET). Thirteen Danish national team orienteering athletes participated in the study. CON athletes adhered to planned physical, cognitive, and technical training. BET athletes added 20 min of route choice assessment (RCA) training after each weekly aerobic training session. The 30 min Stroop color-word task and a sport-specific RCA task evaluated general and sport-specific cognitive performance. A submaximal (1000 m) and a maximal (5000 m) running test were also conducted. Endpoints were assessed pre and post CON and post BET. Results: Average time used per RCA task was 1.4 ± 0.4 s lower following BET (27%) (p = 0.009) compared with no change after CON. Similarly, the total number of correct Stroop answers increased by 13.8 ± 5.21 points (2%) after BET with no change after CON. RCA time use declined steeply from session 1–7, whereafter average time use plateaued. Running performance did not differ significantly between periods. Conclusion: BET improved sport-specific performance and aspects of general cognitive performance, and may effectively improve cognitive parts important for elite orienteering performance. Full article

The validity and accuracy of photoplethysmography (PPG)-based wearable heart rate (HR) monitors remain debatable. This study aimed to determine the concurrent validity of HR records from a wide range of contemporary PPG monitors across the full spectrum of exercise intensities and running conditions. Ten well-trained male endurance athletes performed several incremental maximal aerobic (IMA) tests and high-intensity interval training (HIIT) sessions while wearing a Polar H9 chest strap, as the reference criterion; 16 PPG-based heart rate monitors were tested (Amazfit Fit5; Apple Ultra and SE; Garmin 35, 45, 235, and 935; Polar M200, M430, OH1, Vantage M, Vantage V, Vantage V3, and Verity Sense; Galaxy Watch 5, Suunto 3 Fitness). The results showed excellent ICC (>0.90) versus the reference device across IMA and HIIT tests. Overall, the ICC decreased, and magnitudes of error increased (BIAS, SEM, and CV) as the intensity increased. Moreover, lower ICC values and greater BIAS, SEM, and CV were observed during the HIIT compared to the IMA test. Nevertheless, notable differences between devices were observed in magnitude of errors, accuracy, data loss, and read failures. In conclusion, PPG-based HR monitor validity is device-dependent and therefore exercise data from running training and competitions should be interpreted with caution. Full article

Objectives: This study investigated whether CoQ10 supplementation enhances physical adaptations to high-intensity interval training (HIIT) in muscular strength, power, and physical function in older adults. Method: In a double-blind, randomized controlled trial, 38 adults aged 65–75 were assigned to either a CoQ10 (Females: 8; Males: 11) or placebo (Females: 8; Males: 11) group and completed an 8-week supervised HIIT program. Lower- and upper-body strength (30s 5-repetition chair stand [5XSST], chair standing [30CST], handgrip strength [HGR/L]), balance (single-leg stand [SLS], timed up and go [TUG]), mobility (25-foot walk [25FW]), and aerobic endurance (6-minute walk [6MWT]) were assessed pre- and post-intervention. Results: The CoQ10 group demonstrated significantly greater improvements in 5XSST and 30CST compared to the placebo group (p < 0.05). Both groups showed significant within-group improvements in right and left handgrip strength, SLS, 6MWT, and TUG (all p < 0.001), with no significant between-group differences observed for these outcomes (p > 0.05). No adverse events were reported. Conclusion: While CoQ10 supplementation enhanced improvements in lower-body strength and power, as indicated by the greater gains in 5XSST and 30CST performance compared to the placebo, no between-group differences were observed in TUG, grip strength, or other functional outcomes. This suggests that the performance-related effects of CoQ10 may be more specific to muscular power output and fatigue resistance, rather than general mobility or balance-related tasks. These findings highlight the potential of CoQ10 as a targeted adjunct in exercise for supporting lower-body function and physical performance in older adults. Full article

Background: Obesity during pregnancy increases the risk of adverse maternal and neonatal outcomes, and excessive gestational weight gain (GWG) remains highly prevalent worldwide. Although physical activity (PA) interventions have shown potential benefits, evidence on the optimal type, intensity, and duration of exercise for overweight or obese pregnant women remains limited. Methods: Electronic searches of EBSCOhost, Embase, PubMed and Web of Science were performed through August 2025 to identify randomized controlled trials comparing PA interventions versus usual prenatal care in overweight or obese pregnant women. Two reviewers independently screened studies, extracted data, and assessed risk of bias using Cochrane ROB domains. Continuous outcomes were pooled using inverse-variance meta-analytic methods and heterogeneity was quantified by I². Results: Ten randomized trials (twelve intervention arms) comprising 1150 participants met the inclusion criteria. In the domain of blinding of participants and personnel, three studies (30%) were judged as low risk, while seven (70%) were unclear. PA interventions varied in modality (aerobic, resistance, endurance, walking), setting (clinic, community, home/mHealth), and the intervention period ranges from 10 to 34 weeks. Most interventions (80%) employed moderate intensity, and 30% combined aerobic and resistance training. Results of the meta-analysis showed that the pooled mean GWG was 9.93 ± 5.48 kg in the treatment group and 10.65 ± 5.70 kg in the control group. Overall, PA interventions produced a modest but statistically significant reduction in GWG compared with controls, with negligible between-study heterogeneity (I² = 0%). Conclusions: Tailored, moderate-intensity PA may have the potential to modestly reduce GWG. Although 30% included trials employed combined aerobic and resistance training, current evidence is insufficient to establish whether combined modalities are more effective than aerobic-only or resistance-only interventions. However, the current evidence is limited by small trial sizes, methodological variability and geographic concentration in higher-income settings. Larger, rigorously designed RCTs, including evaluations of digital delivery platforms and carefully supervised higher-intensity protocols, are needed to refine exercise prescriptions and inform clinical guidelines. Full article

Background: Heart failure with reduced ejection fraction (HFrEF) markedly impairs quality of life (QoL) and life expectancy. The main therapeutic goals are to reduce mortality, improve functional capacity, and enhance QoL. Exercise training is an evidence-based, non-pharmacological component of standard care that improves functional capacity and clinical outcomes in HFrEF. This review examines the effects of endurance and resistance training on peak oxygen uptake (VO₂peak), ventilatory efficiency (VE/VCO₂ slope), health-related QoL, and cardiovascular outcomes in patients with HFrEF. Methods: A structured narrative review was conducted using comprehensive searches of PubMed, EMBASE, and the Cochrane Library for English-language studies published between January 2004 and October 2024. Eligible studies included adult HFrEF populations undergoing aerobic and/or resistance training with reported effects on VO₂peak, ventilatory efficiency, QoL, or clinical outcomes. Given the heterogeneity of interventions, comparators, and outcome metrics, data were synthesized descriptively. Results: Across 18 studies (plus one sub-analysis) including 3401 patients, 17 trials assessed VO₂peak and 16 reported significant improvements, with an average increase of approximately 2 mL·kg⁻¹·min⁻¹. Six studies assessed ventilatory efficiency, and five demonstrated reductions in VE/VCO₂ slope averaging 4.4 units. Eleven studies analyzed QoL, and nine reported significant improvements corresponding to an ≈5-point decrease in the Minnesota Living with Heart Failure Questionnaire (MLHFQ). In the largest trial, exercise training was associated with modest but statistically significant reductions in all-cause mortality or hospitalization (HR 0.89) and cardiovascular mortality or heart-failure hospitalization (HR 0.85) after adjustment for baseline prognostic factors. Conclusions: Structured exercise training improves aerobic capacity, ventilatory efficiency, and QoL in patients with HFrEF, with supportive evidence for reduced morbidity and mortality. These findings underscore the value of structured exercise as a core component of modern HFrEF management. Full article

Background: This study aimed to compare the effects of linear sprint training with changes of direction (LSCD) versus small-sided games (SSSG) on physical performance, agility, and soccer-specific skills in young elite female players. Methods: In a randomized crossover study, 27 players aged 15 to 17 were divided into two groups (G1 = 14, G2 = 13). After a two-week baseline period, each group completed a four-week training mesocycle (three sessions per week) consisting of either LSCD or SSG. After a two-week washout period, participants switched interventions and completed the alternate four-week mesocycle. Performance assessments were conducted before and after each mesocycle to evaluate training effects. Results: Both types of training improved physical performance, with different magnitudes. LSCD induced larger gains in sprint speed (5, 10, 20 m; p < 0.05), agility without the ball (t-test; p = 0.05), and explosive power (countermovement jump, repeated jumps over 15 s; p = 0.02 and p = 0.004). In contrast, SSSG led to larger improvements in aerobic endurance (Yo-Yo IR1 test; p = 0.03) and agility with the ball (t-test with ball; p = 0.05). No transfer effect between cycles was observed. Conclusions: In young elite female players, LSCD training was more effective in improving speed, agility, and power, while SSSG was more effective for aerobic endurance and ball agility. Full article

Background: Foam rolling has become an increasingly popular self-myofascial release (SMR) technique among athletes to prevent injuries, improve recovery, and increase athletic performance. This study investigated how SMR improves mechanical and movement efficiency in recreational road cyclists. Methods: We conducted an exploratory randomized controlled trial (RCT) to investigate the effects of SMR using a foam roller on biomechanical and physiological performance parameters over a six-month period. A total of 32 male participants, aged 26–57 years, with a mean Body Mass Index (BMI) of 24.0 kg/m² (SD = 2.2), were randomly assigned to either an intervention group (n = 16), which incorporated a standardized SMR program into their post-exercise recovery, or a control group (n = 16), which followed the same cycling protocol without SMR. The training program included heart rate-controlled strength endurance intervals. As the primary target, the variables we investigated included torque effectiveness, leg force symmetry, and pedal smoothness. Secondary measurements included submaximal oxygen uptake (VO₂) as well as bioelectrical variables, which we analyzed using classic, repeated-measures ANOVA models and descriptive statistical methods. Results: The analysis revealed significant interaction effects in favor of the intervention group for torque effectiveness (η²_p = 0.434), leg strength symmetry (η²_p = 0.303), and pedal smoothness (η²_p = 0.993). No significant group × time interactions were found for submaximal VO₂ or bioelectrical parameters. Conclusions: Our findings indicate that foam rolling may serve as an effective adjunct to endurance training by enhancing functional neuromuscular performance in cyclists, particularly in torque control and pedal coordination. Its impact on aerobic efficiency and muscle composition appears to be minimal. The results support theoretical models that attribute SMR benefits to proprioceptive, circulatory, and neuromuscular mechanisms rather than structural tissue adaptations. Full article

Aging entails concurrent declines in physical and cognitive domains, contributing to reduced independence, higher fall risk, and lower quality of life. Understanding how motor performance relates to cognition is crucial for prevention in community-dwelling older adults. This cross-sectional study investigated associations between physical fitness—including strength, endurance, balance, and aerobic capacity—and cognitive outcomes assessed by Trail Making Tests (TMT-A, TMT-B), the Digit Symbol Substitution Test (DSST), and dual-task cost in the Timed Up & Go Test (TUG_DTC). Thirty-four sedentary but cognitively healthy adults aged ≥60 years were evaluated. Quadriceps strength was significantly related to TUG_DTC and executive function (TMT-B), while upper-limb endurance correlated with both attentional and executive measures. Dynamic balance, particularly in posterior directions, was linked to DSST and TMT-B, and 6-min walk test performance was associated with executive functioning and processing speed. TUG_DTC itself showed strong correlations with cognitive outcomes, underscoring its sensitivity to motor–cognitive interference. These findings highlight selective motor–cognitive relationships and suggest that simple, field-based tests may serve as early markers of cognitive vulnerability. Targeting lower-limb strength, balance, and aerobic capacity could represent cost-effective strategies to promote mobility and cognitive resilience in aging populations. Full article

Caffeine, a nervous system stimulant, and sodium bicarbonate, a metabolic buffer, have been shown to improve performance in high-intensity, particularly aerobic, exercises. This randomized, double-blind, placebo-controlled, crossover study compared the acute effects of caffeine and sodium bicarbonate on chest press (CP) and knee extension (KE) performance. Twelve resistance-trained young adults (seven females) completed three testing sessions during which they consumed caffeine (0.003 g/kg of body weight), sodium bicarbonate (0.3 g/kg of body weight), or placebo at 120, 90, and 60 min prior to testing. Testing consisted of six sets of CP and KE for as many repetitions as possible at 70% of the participants’ 1-repetition maximum load. A 60 s recovery between sets, and 2 min recovery was allocated between exercises. Blood pressure and blood lactate were recorded pre-, mid-, and post-test. Heart rate, ratings of perceived exertion (RPE), repetitions, and electromyography (EMG) were recorded for every set. No significant differences were found under any condition for RPE, EMG, and KE repetitions. A main effect for groups revealed was increased CP repetitions with sodium bicarbonate (7.42; 95%CI: 6.8–7.9) versus caffeine (6.7; 95%CI: 6.1–7.3) and control (7.1; 95%CI: 6.4–7.6) conditions. However, post hoc analysis did not achieve significance. Diastolic blood pressure was significantly (p = 0.03) greater with caffeine (79.2 mmHg; 95%CI: 74.6–83.7) than with sodium bicarbonate (72.7 mmHg; 95%CI: 67.5–77.9) and control (74.5 mmHg; 95%CI: 71.7–77.3). Females had significantly lower blood lactate measurements, higher CP repetitions, and lower heart rates, despite similar resting heart rates between the sexes. Caffeine (0.003 g/kg) or sodium bicarbonate (0.3 g/kg) did not provide acute ergogenic effects on CP or KE strength endurance (six sets of CP and KE at 70% 1-repetition maximum load) performance in young adult women and men. Full article

Accurate assessment of aerobic fitness is crucial in soccer; however, the validity of field-based predictive tests remains uncertain in professional players. This study examined the relationship between directly measured and estimated maximal oxygen uptake ($\dot{\mathrm{V}}$O_2max) during the Yo-Yo Endurance Test Level 1 (YYET₁) in professional soccer players and evaluated seasonal changes after six months of training and competition. Seventeen players from an Italian third-division team performed the YYET₁ in pre- and mid-season conditions, while VO_2max was continuously recorded using a portable metabolic system. VO_2max was estimated using Bangsbo’s distance-based formula. Linear regression and Bland–Altman analyses were used to assess relationships and agreement between methods. Measured VO_2max increased significantly from pre- to mid- season (+13.9%, p < 0.001), whereas estimated values showed a smaller rise (+5.2%, p < 0.001). The predictive method systematically underestimated VO_2max (bias −2.3 to −7.0 mL·kg⁻¹·min⁻¹), and regression analyses revealed only moderate shared variance (R² = 0.18–0.20) between estimated and measured values. These findings demonstrate that Bangsbo’s equation lacks validity for estimating VO_2max in professional players and cannot accurately track aerobic adaptations across a season. For precise physiological evaluation, direct measurement using portable metabolic systems is required, while submaximal soccer-specific protocols may offer practical alternatives for longitudinal monitoring. Full article

Background/Objectives: Hypertension is a leading cause of cardiovascular morbidity and mortality, particularly in older adults, pharmacological therapy is effective, but side effects and limited adherence highlight the need for non-pharmacological alternatives. This study investigated the effects of a structured Bodypump (BoP) programme, a choreographed group-based resistance training intervention, on blood pressure (BP) and functional fitness in sedentary older adults with hypertension. Methods: Thirty-two participants (65.4 ± 7.7 years) diagnosed with hypertension were randomly allocated to a BoP group (n = 16) or a control group (CG) (n = 16). The intervention lasted 8-week and consisted of 3 supervised sessions per week. Resting systolic BP (SBP) and diastolic BP (DBP) were measured using a validated automated device (Omron M3 Intellisense, HEM-7051-E), functional capacity was assessed with the Senior Fitness Test (SFT) battery. Results: After the intervention, BoP exhibited significant reductions in SBP (−24.4 ± 4.7 mmHg; p < 0.001) and DBP (−6.4 ± 2.7 mmHg; p = 0.025) compared to CG. BoP improved functional fitness, lower- and upper-body strength, aerobic endurance and agility (p < 0.05), with no changes in the CG. Conclusions: 8-week of BoP programme reduced BP and enhanced physical function in sedentary hypertensive older adults. Given its accessible, motivating and socially engaging format, BoP represents a promising non-pharmacological strategy for hypertension management and functional fitness of healthy ageing. Full article

The problems of food waste and packaging waste production currently force us to search for new solutions that are safe for people and the environment. Applying edible coatings directly onto food offers a sustainable method of maintaining shrimp freshness, eliminating the need for artificial preservatives and avoiding the high energy demands of conventional chemical or physical preservation techniques. In this study, starch materials modified with natural extracts from plants with proven health-promoting and antibacterial properties—rooibos and garlic—were obtained and tested. The structure, hydrophilicity, water vapor permeability, and thermal and mechanical properties of the obtained starch films were determined. The study further revealed that Escherichia coli was absent in all shrimp samples coated with starch-based films following seven days of refrigerated storage, in contrast to uncoated samples. For Staphylococcus aureus, coatings with rooibos extract resulted in a significant reduction in bacterial counts. Coatings with garlic extract showed a marginally reduced antibacterial effect. The effect of the coatings on the overall numbers of lactic acid bacteria and aerobic mesophilic bacteria was evaluated as well. Coatings enriched with rooibos were more effective in the early days of storage, while garlic-based coatings exhibited a less intense but more enduring antimicrobial effect. Full article

Colombian Paso horses (CPs) excel in gait competitions due to their endurance, speed, and precision, which demand a high level of cardiorespiratory fitness. However, their training regimes often lack scientific support, and few studies have linked physiological parameters to optimal training zones. This study aimed to estimate the aerobic lactate threshold (LTaer) using mathematical methods and to correlate it with heart rate (HR) zones and blood lactate. Eighteen CPs were evaluated and classified into trained (GT) and untrained (GD) groups. All animals underwent a field-based incremental exercise test (IET) with continuous HR monitoring and serial blood sampling for lactate, creatinine, BUN, AST, and CK analysis. LTaer was estimated using three methods: visual inspection, and fixed thresholds at 2 mmol/L (ZL2), and 4 mmol/L (ZL4). HR zones corresponding to each threshold were then calculated based on a maximum estimated HR of 220 bpm. The visual method placed LTaer in zone 2 for both groups. ZL2 located the threshold in zone 2 for GT and in zone 3 for GD, whereas ZL4 placed the threshold in zone 4 for both groups. Although no intergroup differences in lactate or HR were observed, intragroup differences emerged above 75% of HRmax, indicating exponential lactate accumulation. Biochemical parameters revealed significant pre- to post-exercise changes, but no differences between groups, suggesting a standardized workload. This is the first study to propose field-based prediction of LTaer in CPs using HR derived from wearable technologies, based on blood lactate and a standardized exercise test’s results. Correlating HR zones with lactate thresholds facilitates workload analysis in the field. Future studies should investigate the maximal lactate steady state (MLSS) in this breed. Full article