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Search Results (3,441)

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25 pages, 861 KiB  
Article
Designing a Board Game to Expand Knowledge About Parental Involvement in Teacher Education
by Zsófia Kocsis, Zsolt Csák, Dániel Bodnár and Gabriella Pusztai
Educ. Sci. 2025, 15(8), 986; https://doi.org/10.3390/educsci15080986 (registering DOI) - 2 Aug 2025
Abstract
Research highlights a growing demand for active, experiential learning methods in higher education, especially in teacher education. While the benefits of parental involvement (PI) are well-documented, Hungary lacks tools to effectively prepare teacher trainees for fostering family–school cooperation. This study addresses this gap [...] Read more.
Research highlights a growing demand for active, experiential learning methods in higher education, especially in teacher education. While the benefits of parental involvement (PI) are well-documented, Hungary lacks tools to effectively prepare teacher trainees for fostering family–school cooperation. This study addresses this gap by introducing a custom-designed board game as an innovative teaching tool. The game simulates real-world challenges in PI through a cooperative, scenario-based framework. Exercises are grounded in international and national research, ensuring their relevance and evidence-based design. Tested with 110 students, the game’s educational value was assessed via post-gameplay questionnaires. Participants emphasized the strengths of its cooperative structure, realistic scenarios, and integration of humor. Many reported gaining new insights into parental roles and strategies for effective home–school partnerships. Practical applications include integrating the game into teacher education curricula and adapting it for other educational contexts. This study demonstrates how board games can bridge theory and practice, offering an engaging, effective medium to prepare future teachers for the challenges of PI. Full article
(This article belongs to the Section Teacher Education)
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25 pages, 1138 KiB  
Article
Quality over Quantity: An Effective Large-Scale Data Reduction Strategy Based on Pointwise V-Information
by Fei Chen and Wenchi Zhou
Electronics 2025, 14(15), 3092; https://doi.org/10.3390/electronics14153092 (registering DOI) - 1 Aug 2025
Abstract
In order to increase the effectiveness of model training, data reduction is essential to data-centric Artificial Intelligence (AI). It achieves this by locating the most instructive examples in massive datasets. To increase data quality and training efficiency, the main difficulty is choosing the [...] Read more.
In order to increase the effectiveness of model training, data reduction is essential to data-centric Artificial Intelligence (AI). It achieves this by locating the most instructive examples in massive datasets. To increase data quality and training efficiency, the main difficulty is choosing the best examples rather than the complete datasets. In this paper, we propose an effective data reduction strategy based on Pointwise 𝒱-Information (PVI). To enable a static method, we first use PVI to quantify instance difficulty and remove instances with low difficulty. Experiments show that classifier performance is maintained with only a 0.0001% to 0.76% decline in accuracy when 10–30% of the data is removed. Second, we train the classifiers using a progressive learning strategy on examples sorted by increasing PVI, accelerating convergence and achieving a 0.8% accuracy gain over conventional training. Our findings imply that training a classifier on the chosen optimal subset may improve model performance and increase training efficiency when combined with an efficient data reduction strategy. Furthermore, we have adapted the PVI framework, which was previously limited to English datasets, to a variety of Chinese Natural Language Processing (NLP) tasks and base models, yielding insightful results for faster training and cross-lingual data reduction. Full article
(This article belongs to the Special Issue Data Retrieval and Data Mining)
18 pages, 12398 KiB  
Article
Optimizing Advertising Billboard Coverage in Urban Networks: A Population-Weighted Greedy Algorithm with Spatial Efficiency Enhancements
by Jiaying Fu and Kun Qin
ISPRS Int. J. Geo-Inf. 2025, 14(8), 300; https://doi.org/10.3390/ijgi14080300 (registering DOI) - 1 Aug 2025
Abstract
The strategic allocation of advertising billboards has become a critical aspect of urban planning and resource management. While previous studies have explored site selection based on road network and population data, they have often overlooked the diminishing marginal returns of overlapping coverage and [...] Read more.
The strategic allocation of advertising billboards has become a critical aspect of urban planning and resource management. While previous studies have explored site selection based on road network and population data, they have often overlooked the diminishing marginal returns of overlapping coverage and neglected to efficiently process large-scale urban datasets. To address these challenges, this study proposes two complementary optimization methods: an enhanced greedy algorithm based on geometric modeling and spatial acceleration techniques, and a reinforcement learning approach using Proximal Policy Optimization (PPO). The enhanced greedy algorithm incorporates population-weighted road coverage modeling, employs a geometric series to capture diminishing returns from overlapping coverage, and integrates spatial indexing and parallel computing to significantly improve scalability and solution quality in large urban networks. Meanwhile, the PPO-based method models billboard site selection as a sequential decision-making process in a dynamic environment, where agents adaptively learn optimal deployment strategies through reward signals, balancing coverage gains and redundancy penalties and effectively handling complex multi-step optimization tasks. Experiments conducted on Wuhan’s road network demonstrate that both methods effectively optimize population-weighted billboard coverage under budget constraints while enhancing spatial distribution balance. Quantitatively, the enhanced greedy algorithm improves coverage effectiveness by 18.6% compared to the baseline, while the PPO-based method further improves it by 4.3% with enhanced spatial equity. The proposed framework provides a robust and scalable decision-support tool for urban advertising infrastructure planning and resource allocation. Full article
28 pages, 17610 KiB  
Article
Histological Assessment of Intestinal Changes Induced by Liquid Whey-Enriched Diets in Pigs
by Kamel Mhalhel, Mauro Cavallaro, Lidia Pansera, Leyanis Herrera Ledesma, Maria Levanti, Antonino Germanà, Anna Maria Sutera, Giuseppe Tardiolo, Alessandro Zumbo, Marialuisa Aragona and Giuseppe Montalbano
Vet. Sci. 2025, 12(8), 716; https://doi.org/10.3390/vetsci12080716 - 30 Jul 2025
Viewed by 194
Abstract
Liquid whey (LW) is a nutrient-rich dairy by-product and a promising resource for animal nutrition. However, data regarding its impact on intestinal morphology and endocrine signaling are limited. Therefore, the current study aims to dissect those aspects. An experiment was conducted on 14 [...] Read more.
Liquid whey (LW) is a nutrient-rich dairy by-product and a promising resource for animal nutrition. However, data regarding its impact on intestinal morphology and endocrine signaling are limited. Therefore, the current study aims to dissect those aspects. An experiment was conducted on 14 crossbred pigs divided into control (fed 3% of their body weight pelleted feed) and LW (fed 3% of their body weight supplemented with 1.5 L of LW) groups. The results show a significantly increased body weight gain in LW pigs during the second half of the experiment. Moreover, an increased ileal villus height, deeper crypts, and a thicker muscularis externa in the duodenum and jejunum have been reported in LW-fed pigs. Goblet cell count revealed a significant abundance of these cells in duodenal villi and jejunal crypts of the LW group, suggesting enhanced mucosal defense in all segments of LW-fed pigs. While Cholecystokinin8 and Galanin showed the same expression pattern among both groups and SI segments, the leptin expression was significantly higher in LW swine. These findings indicate that LW promotes growth, gut mucosa remodeling, and neuroendocrine signaling, thus supporting LW use as a functional dietary strategy with attention to the adaptation period. Full article
(This article belongs to the Section Anatomy, Histology and Pathology)
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24 pages, 2070 KiB  
Article
Reinforcement Learning-Based Finite-Time Sliding-Mode Control in a Human-in-the-Loop Framework for Pediatric Gait Exoskeleton
by Matthew Wong Sang and Jyotindra Narayan
Machines 2025, 13(8), 668; https://doi.org/10.3390/machines13080668 - 30 Jul 2025
Viewed by 158
Abstract
Rehabilitation devices such as actuated lower-limb exoskeletons can provide essential mobility assistance for pediatric patients with gait impairments. Enhancing their control systems under conditions of user variability and dynamic disturbances remains a significant challenge, particularly in active-assist modes. This study presents a human-in-the-loop [...] Read more.
Rehabilitation devices such as actuated lower-limb exoskeletons can provide essential mobility assistance for pediatric patients with gait impairments. Enhancing their control systems under conditions of user variability and dynamic disturbances remains a significant challenge, particularly in active-assist modes. This study presents a human-in-the-loop control architecture for a pediatric lower-limb exoskeleton, combining outer-loop admittance control with robust inner-loop trajectory tracking via a non-singular terminal sliding-mode (NSTSM) controller. Designed for active-assist gait rehabilitation in children aged 8–12 years, the exoskeleton dynamically responds to user interaction forces while ensuring finite-time convergence under system uncertainties. To enhance adaptability, we augment the inner-loop control with a twin delayed deep deterministic policy gradient (TD3) reinforcement learning framework. The actor–critic RL agent tunes NSTSM gains in real-time, enabling personalized model-free adaptation to subject-specific gait dynamics and external disturbances. The numerical simulations show improved trajectory tracking, with RMSE reductions of 27.82% (hip) and 5.43% (knee), and IAE improvements of 40.85% and 10.20%, respectively, over the baseline NSTSM controller. The proposed approach also reduced the peak interaction torques across all the joints, suggesting more compliant and comfortable assistance for users. While minor degradation is observed at the ankle joint, the TD3-NSTSM controller demonstrates improved responsiveness and stability, particularly in high-load joints. This research contributes to advancing pediatric gait rehabilitation using RL-enhanced control, offering improved mobility support and adaptive rehabilitation outcomes. Full article
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14 pages, 1726 KiB  
Systematic Review
Mucous Fistula Refeeding in Newborns: Why, When, How, and Where? Insights from a Systematic Review
by Layla Musleh, Ilaria Cozzi, Anteo Di Napoli and Fabio Fusaro
Nutrients 2025, 17(15), 2490; https://doi.org/10.3390/nu17152490 - 30 Jul 2025
Viewed by 163
Abstract
Background/Objectives: Infants with high-output enterostomies often require prolonged parenteral nutrition (PN), increasing risks of infections, liver dysfunction, and impaired growth. Mucous fistula refeeding (MFR) is proposed to enhance intestinal adaptation, weight gain, and distal bowel maturation. This systematic review and meta-analysis assessed [...] Read more.
Background/Objectives: Infants with high-output enterostomies often require prolonged parenteral nutrition (PN), increasing risks of infections, liver dysfunction, and impaired growth. Mucous fistula refeeding (MFR) is proposed to enhance intestinal adaptation, weight gain, and distal bowel maturation. This systematic review and meta-analysis assessed its effectiveness, safety, and technical aspects. Methods: Following PRISMA guidelines, studies reporting MFR-related outcomes were included without data or language restrictions. Data sources included PubMed, EMBASE, CINAHL, Scopus, Web of Science, Cochrane Library, and UpToDate. Bias risk was assessed using the Joanna Briggs Institute Critical Appraisal Checklist. Meta-analysis employed random- and fixed-effects models, with outcomes reported as odds ratios (ORs) and 95% confidence interval (CI). Primary outcomes assessed were weight gain, PN duration, and complications and statistical comparisons were made between MFR and non-MFR groups. Results: Seventeen studies involving 631 infants were included; 482 received MFR and 149 did not. MFR started at 31 postoperative days and lasted for 50 days on average, using varied reinfusion methods, catheter types, and fixation strategies. MFR significantly improved weight gain (4.7 vs. 24.2 g/day, p < 0.05) and reduced PN duration (60.3 vs. 95 days, p < 0.05). Hospital and NICU stays were also shorter (160 vs. 263 days, p < 0.05; 122 vs. 200 days, p < 0.05). Cholestasis risk was lower (OR 0.151, 95% CI 0.071–0.319, p < 0.0001), while effects on bilirubin levels were inconsistent. Complications included sepsis (3.5%), intestinal perforation (0.83%), hemorrhage (0.62%), with one MFR-related death (0.22%). Conclusions: Despite MFR benefits neonatal care, its practices remain heterogeneous. Standardized protocols are required to ensure MFR safety and efficacy. Full article
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24 pages, 1599 KiB  
Article
Climate-Regulating Industrial Ecosystems: An AI-Optimised Framework for Green Infrastructure Performance
by Shamima Rahman, Ali Ahsan and Nazrul Islam Pramanik
Sustainability 2025, 17(15), 6891; https://doi.org/10.3390/su17156891 - 29 Jul 2025
Viewed by 185
Abstract
This paper presents an Industrial–Ecological Symbiosis Framework that enables industrial operations to achieve quantifiable ecological gains without compromising operational efficiency. The model integrates Mixed-Integer Linear Programming (MILP) with AI-optimised forecasting to allow real-time adjustments to production and resource use. It was tested across [...] Read more.
This paper presents an Industrial–Ecological Symbiosis Framework that enables industrial operations to achieve quantifiable ecological gains without compromising operational efficiency. The model integrates Mixed-Integer Linear Programming (MILP) with AI-optimised forecasting to allow real-time adjustments to production and resource use. It was tested across the apparel manufacturing, metalworking, and mining sectors using publicly available benchmark datasets. The framework delivered consistent improvements: fabric waste was reduced by 10.8%, energy efficiency increased by 15%, and carbon emissions decreased by 14%. These gains were statistically validated and quantified using ecological equivalence metrics, including forest carbon sequestration rates and wetland restoration values. Outputs align with national carbon accounting systems, SDG reporting, and policy frameworks—specifically contributing to SDGs 6, 9, and 11–13. By linking industrial decisions directly to verified environmental outcomes, this study demonstrates how adaptive optimisation can support climate goals while maintaining productivity. The framework offers a reproducible, cross-sectoral solution for sustainable industrial development. Full article
(This article belongs to the Section Environmental Sustainability and Applications)
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28 pages, 3144 KiB  
Review
Artificial Intelligence-Driven and Bio-Inspired Control Strategies for Industrial Robotics: A Systematic Review of Trends, Challenges, and Sustainable Innovations Toward Industry 5.0
by Claudio Urrea
Machines 2025, 13(8), 666; https://doi.org/10.3390/machines13080666 - 29 Jul 2025
Viewed by 388
Abstract
Industrial robots are undergoing a transformative shift as Artificial Intelligence (AI)-driven and bio-inspired control strategies unlock new levels of precision, adaptability, and multi-dimensional sustainability aligned with Industry 5.0 (energy efficiency, material circularity, and life-cycle emissions). This systematic review analyzes 160 peer-reviewed industrial robotics [...] Read more.
Industrial robots are undergoing a transformative shift as Artificial Intelligence (AI)-driven and bio-inspired control strategies unlock new levels of precision, adaptability, and multi-dimensional sustainability aligned with Industry 5.0 (energy efficiency, material circularity, and life-cycle emissions). This systematic review analyzes 160 peer-reviewed industrial robotics control studies (2023–2025), including an expanded bio-inspired/human-centric subset, to evaluate: (1) the dominant and emerging control methodologies; (2) the transformative role of digital twins and 5G-enabled connectivity; and (3) the persistent technical, ethical, and environmental challenges. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines, the study employs a rigorous methodology, focusing on adaptive control, deep reinforcement learning (DRL), human–robot collaboration (HRC), and quantum-inspired algorithms. The key findings highlight up to 30% latency reductions in real-time optimization, up to 22% efficiency gains through digital twins, and up to 25% energy savings from bio-inspired designs (all percentage ranges are reported relative to the comparator baselines specified in the cited sources). However, critical barriers remain, including scalability limitations (with up to 40% higher computational demands) and cybersecurity vulnerabilities (with up to 20% exposure rates). The convergence of AI, bio-inspired systems, and quantum computing is poised to enable sustainable, autonomous, and human-centric robotics, yet requires standardized safety frameworks and hybrid architectures to fully support the transition from Industry 4.0 to Industry 5.0. This review offers a strategic roadmap for future research and industrial adoption, emphasizing human-centric design, ethical frameworks, and circular-economy principles to address global manufacturing challenges. Full article
(This article belongs to the Section Robotics, Mechatronics and Intelligent Machines)
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25 pages, 4407 KiB  
Article
A Reproducible Pipeline for Leveraging Operational Data Through Machine Learning in Digitally Emerging Urban Bus Fleets
by Bernardo Tormos, Vicente Bermudez, Ramón Sánchez-Márquez and Jorge Alvis
Appl. Sci. 2025, 15(15), 8395; https://doi.org/10.3390/app15158395 - 29 Jul 2025
Viewed by 168
Abstract
The adoption of predictive maintenance in public transportation has gained increasing attention in the context of Industry 4.0. However, many urban bus fleets remain in early digital transformation stages, with limited historical data and fragmented infrastructures that hinder the implementation of data-driven strategies. [...] Read more.
The adoption of predictive maintenance in public transportation has gained increasing attention in the context of Industry 4.0. However, many urban bus fleets remain in early digital transformation stages, with limited historical data and fragmented infrastructures that hinder the implementation of data-driven strategies. This study proposes a reproducible Machine Learning pipeline tailored to such data-scarce conditions, integrating domain-informed feature engineering, lightweight and interpretable models (Linear Regression, Ridge Regression, Decision Trees, KNN), SMOGN for imbalance handling, and Leave-One-Out Cross-Validation for robust evaluation. A scheduled batch retraining strategy is incorporated to adapt the model as new data becomes available. The pipeline is validated using real-world data from hybrid diesel buses, focusing on the prediction of time spent in critical soot accumulation zones of the Diesel Particulate Filter (DPF). In Zone 4, the model continued to outperform the baseline during the production test, indicating its validity for an additional operational period. In contrast, model performance in Zone 3 deteriorated over time, triggering retraining. These results confirm the pipeline’s ability to detect performance drift and support predictive maintenance decisions under evolving operational constraints. The proposed framework offers a scalable solution for digitally emerging fleets. Full article
(This article belongs to the Special Issue Big-Data-Driven Advances in Smart Maintenance and Industry 4.0)
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16 pages, 1170 KiB  
Article
LoRA-Tuned Multimodal RAG System for Technical Manual QA: A Case Study on Hyundai Staria
by Yerin Nam, Hansun Choi, Jonggeun Choi and Hyukjin Kwon
Appl. Sci. 2025, 15(15), 8387; https://doi.org/10.3390/app15158387 - 29 Jul 2025
Viewed by 166
Abstract
This study develops a domain-adaptive multimodal RAG (Retrieval-Augmented Generation) system to improve the accuracy and efficiency of technical question answering based on large-scale structured manuals. Using Hyundai Staria maintenance documents as a case study, we extracted text and images from PDF manuals and [...] Read more.
This study develops a domain-adaptive multimodal RAG (Retrieval-Augmented Generation) system to improve the accuracy and efficiency of technical question answering based on large-scale structured manuals. Using Hyundai Staria maintenance documents as a case study, we extracted text and images from PDF manuals and constructed QA, RAG, and Multi-Turn datasets to reflect realistic troubleshooting scenarios. To overcome limitations of baseline RAG models, we proposed an enhanced architecture that incorporates sentence-level similarity annotations and parameter-efficient fine-tuning via LoRA (Low-Rank Adaptation) using the bLLossom-8B language model and BAAI-bge-m3 embedding model. Experimental results show that the proposed system achieved improvements of 3.0%p in BERTScore, 3.0%p in cosine similarity, and 18.0%p in ROUGE-L compared to existing RAG systems, with notable gains in image-guided response accuracy. A qualitative evaluation by 20 domain experts yielded an average satisfaction score of 4.4 out of 5. This study presents a practical and extensible AI framework for multimodal document understanding, with broad applicability across automotive, industrial, and defense-related technical documentation. Full article
(This article belongs to the Special Issue Innovations in Artificial Neural Network Applications)
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17 pages, 661 KiB  
Article
Adaptive Learning Control for Vehicle Systems with an Asymmetric Control Gain Matrix and Non-Uniform Trial Lengths
by Yangbo Tang, Zetao Chen and Hongjun Wu
Symmetry 2025, 17(8), 1203; https://doi.org/10.3390/sym17081203 - 29 Jul 2025
Viewed by 75
Abstract
Intelligent driving is a key technology in the field of automotive manufacturing due to its advantages in environmental protection, energy efficiency, and economy. However, since the intelligent driving model is an uncertain multi-input multi-output dynamic system, especially in an interactive environment, it faces [...] Read more.
Intelligent driving is a key technology in the field of automotive manufacturing due to its advantages in environmental protection, energy efficiency, and economy. However, since the intelligent driving model is an uncertain multi-input multi-output dynamic system, especially in an interactive environment, it faces uncertainties such as non-uniform trial lengths, unknown nonlinear parameters, and unknown control direction. In this paper, an adaptive iterative learning control method is proposed for vehicle systems with non-uniform trial lengths and asymmetric control gain matrices. Unlike the existing research on adaptive iterative learning for non-uniform test lengths, this paper assumes that the elements of the system’s control gain matrix are asymmetric. Therefore, the assumption made in traditional adaptive iterative learning methods that the control gain matrix of the system is known or real, symmetric, and positive definite (or negative definite) is relaxed. Finally, to prove the convergence of the system, a composite energy function is designed, and the effectiveness of the adaptive iterative learning method is verified using vehicle systems. This paper aims to address the challenges in intelligent driving control and decision-making caused by environmental and system uncertainties and provides a theoretical basis and technical support for intelligent driving, promoting the high-quality development of intelligent transportation. Full article
(This article belongs to the Special Issue Symmetry and Asymmetry in Intelligent Control and Computing)
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25 pages, 3625 KiB  
Article
Automated Classification of Public Transport Complaints via Text Mining Using LLMs and Embeddings
by Daniyar Rakhimzhanov, Saule Belginova and Didar Yedilkhan
Information 2025, 16(8), 644; https://doi.org/10.3390/info16080644 - 29 Jul 2025
Viewed by 157
Abstract
The proliferation of digital public service platforms and the expansion of e-government initiatives have significantly increased the volume and diversity of citizen-generated feedback. This trend emphasizes the need for classification systems that are not only tailored to specific administrative domains but also robust [...] Read more.
The proliferation of digital public service platforms and the expansion of e-government initiatives have significantly increased the volume and diversity of citizen-generated feedback. This trend emphasizes the need for classification systems that are not only tailored to specific administrative domains but also robust to the linguistic, contextual, and structural variability inherent in user-submitted content. This study investigates the comparative effectiveness of large language models (LLMs) alongside instruction-tuned embedding models in the task of categorizing public transportation complaints. LLMs were tested using a few-shot inference, where classification is guided by a small set of in-context examples. Embedding models were assessed under three paradigms: label-only zero-shot classification, instruction-based classification, and supervised fine-tuning. Results indicate that fine-tuned embeddings can achieve or exceed the accuracy of LLMs, reaching up to 90 percent, while offering significant reductions in inference latency and computational overhead. E5 embeddings showed consistent generalization across unseen categories and input shifts, whereas BGE-M3 demonstrated measurable gains when adapted to task-specific distributions. Instruction-based classification produced lower accuracy for both models, highlighting the limitations of prompt conditioning in isolation. These findings position multilingual embedding models as a viable alternative to LLMs for classification at scale in data-intensive public sector environments. Full article
(This article belongs to the Special Issue Text Mining: Challenges, Algorithms, Tools and Applications)
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28 pages, 2854 KiB  
Article
Real-Time Functional Stratification of Tumor Cell Lines Using a Non-Cytotoxic Phospholipoproteomic Platform: A Label-Free Ex Vivo Model
by Ramón Gutiérrez-Sandoval, Francisco Gutiérrez-Castro, Natalia Muñoz-Godoy, Ider Rivadeneira, Adolay Sobarzo, Jordan Iturra, Ignacio Muñoz, Cristián Peña-Vargas, Matías Vidal and Francisco Krakowiak
Biology 2025, 14(8), 953; https://doi.org/10.3390/biology14080953 - 28 Jul 2025
Viewed by 180
Abstract
The development of scalable, non-invasive tools to assess tumor responsiveness to structurally active immunoformulations remains a critical unmet need in solid tumor immunotherapy. Here, we introduce a real-time, ex vivo functional system to classify tumor cell lines exposed to a phospholipoproteomic platform, without [...] Read more.
The development of scalable, non-invasive tools to assess tumor responsiveness to structurally active immunoformulations remains a critical unmet need in solid tumor immunotherapy. Here, we introduce a real-time, ex vivo functional system to classify tumor cell lines exposed to a phospholipoproteomic platform, without relying on cytotoxicity, co-culture systems, or molecular profiling. Tumor cells were monitored using IncuCyte® S3 (Sartorius) real-time imaging under ex vivo neutral conditions. No dendritic cell components or immune co-cultures were used in this mode. All results are derived from direct tumor cell responses to structurally active formulations. Using eight human tumor lines, we captured proliferative behavior, cell death rates, and secretomic profiles to assign each case into stimulatory, inhibitory, or neutral categories. A structured decision-tree logic supported the classification, and a Functional Stratification Index (FSI) was computed to quantify the response magnitude. Inhibitory lines showed early divergence and high IFN-γ/IL-10 ratios; stimulatory ones exhibited a proliferative gain under balanced immune signaling. The results were reproducible across independent batches. This system enables quantitative phenotypic screening under standardized, marker-free conditions and offers an adaptable platform for functional evaluation in immuno-oncology pipelines where traditional cytotoxic endpoints are insufficient. This approach has been codified into the STIP (Structured Traceability and Immunophenotypic Platform), supporting reproducible documentation across tumor models. This platform contributes to upstream validation logic in immuno-oncology workflows and supports early-stage regulatory documentation. Full article
(This article belongs to the Section Cancer Biology)
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26 pages, 1300 KiB  
Review
The Human Mycobiome: Composition, Immune Interactions, and Impact on Disease
by Laura Carrillo-Serradell, Jade Liu-Tindall, Violeta Planells-Romeo, Lucía Aragón-Serrano, Marcos Isamat, Toni Gabaldón, Francisco Lozano and María Velasco-de Andrés
Int. J. Mol. Sci. 2025, 26(15), 7281; https://doi.org/10.3390/ijms26157281 - 28 Jul 2025
Viewed by 522
Abstract
The fungal component of microbiota, known as the mycobiome, inhabits different body niches such as the skin and the gastrointestinal, respiratory, and genitourinary tracts. Much information has been gained on the bacterial component of the human microbiota, but the mycobiome has remained somewhat [...] Read more.
The fungal component of microbiota, known as the mycobiome, inhabits different body niches such as the skin and the gastrointestinal, respiratory, and genitourinary tracts. Much information has been gained on the bacterial component of the human microbiota, but the mycobiome has remained somewhat elusive due to its sparsity, variability, susceptibility to environmental factors (e.g., early life colonization, diet, or pharmacological treatments), and the specific in vitro culture challenges. Functionally, the mycobiome is known to play a role in modulating innate and adaptive immune responses by interacting with microorganisms and immune cells. The latter elicits anti-fungal responses via the recognition of specific fungal cell-wall components (e.g., β-1,3-glucan, mannan, and chitin) by immune system receptors. These receptors then regulate the activation and differentiation of many innate and adaptive immune cells including mucocutaneous cell barriers, macrophages, neutrophils, dendritic cells, natural killer cells, innate-like lymphoid cells, and T and B lymphocytes. Mycobiome disruptions have been correlated with various diseases affecting mostly the brain, lungs, liver and pancreas. This work reviews our current knowledge on the mycobiome, focusing on its composition, research challenges, conditioning factors, interactions with the bacteriome and the immune system, and the known mycobiome alterations associated with disease. Full article
(This article belongs to the Section Molecular Biology)
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14 pages, 2700 KiB  
Article
Seasonal Spatial Distribution Patterns of the Sand Crab Ovalipes punctatus (De Haan 1833) in the Southern Yellow and East China Seas and Predictions from Various Climate Scenarios
by Min Xu, Jianzhong Ling, Haisu Zheng, Xiaojing Song, Zunlei Liu and Huiyu Li
Biology 2025, 14(8), 947; https://doi.org/10.3390/biology14080947 - 28 Jul 2025
Viewed by 276
Abstract
In the past two decades, little information has been updated to understand the resource status of the crab species Ovalipes punctatus in the East China Sea Region. In this study, we conducted surveys in 2018 and 2019 to identify the seasonal spatial distribution [...] Read more.
In the past two decades, little information has been updated to understand the resource status of the crab species Ovalipes punctatus in the East China Sea Region. In this study, we conducted surveys in 2018 and 2019 to identify the seasonal spatial distribution patterns of the economically important sand crab Ovalipes punctatus (De Haan 1833) in the southern Yellow and East China Seas. In the study area, the largest biomass of crabs was observed in the fishing grounds of Dasha and the Yangtze River mouth, and the second largest biomass was detected in the Jiangwai-Zhouwai area. Seasonally, the total biomass order in these areas was summer > autumn & winter > spring, and the mean average individual weight order was spring & summer > winter > autumn. These findings provided maps of the seasonal spatial distribution pattern of the species across seasons, which were then used in climate-change scenario models. Model predictions suggested that O. punctatus might migrate northward and offshore under climate warming conditions, and that the climate scenario SSP585-2100 might be the most negative case, respectively, for the habitat area of gain% minus loss%. These data can be used to develop robust and systematic regional fisheries resource management policies that consider adaptation measures to address the impact of environmental and climate change along China’s coasts and other areas in the world. Full article
(This article belongs to the Special Issue Global Fisheries Resources, Fisheries, and Carbon-Sink Fisheries)
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