Search Results (70)

High-resolution CTD profiles, with SVP cross-validation of the sound speed field, were recorded at a single station in the outer roadstead of the Port of Constanța (northwest Black Sea; 44°07′41″ N, 28°53′15″ E; depth ≈ 25 m; June 2024), revealing a strongly stratified, five-layer water column driven by three combined forcing mechanisms: seasonal thermal stratification with an abnormally shallow Cold Intermediate Water layer (7.3–15.6 m), Danube-sourced freshwater input, and anthropogenic disturbances consistent with port and anchorage activity. A contextual hypothesis is proposed that conflict-related marine traffic intensification may contribute to observed signals, but physical measurements cannot establish causation. At the main pycnocline (7.31–15.62 m), a density difference of Δρ = 4.02 kg m⁻³ yields a maximum Brunt–Väisälä frequency of N² = 2.37 × 10⁻³ s⁻², reducing vertical eddy diffusivity by two orders of magnitude ($Kz$ ≈ 10⁻⁶ m² s⁻¹). Physical conditions—a shallow mixed layer (~0.7–1.2 m) and strong pycnocline—support the theoretical expectation of surface-layer contaminant accumulation; however, no chemical measurements were carried out to confirm contaminant presence. All contamination inferences rely exclusively on physical proxies (turbidity, dissolved oxygen, and density gradients), and contaminant retention remains untested for lack of direct chemical evidence. A dimensional Stratification-Controlled Retention Index (SCRI = N²/Kz; units: m⁻² s⁻¹) is introduced, and its consistency with the observed hydrographic structure is demonstrated. Full article

Short-term captivity is widely used in experimental studies but may unintentionally alter host-associated microbiomes, potentially confounding biological interpretation of experimental outcomes. Here, we evaluated the effects of 35 days of captivity on the gut microbiome of Fundulus heteroclitus collected from Long Island Sound (Milford, CT, USA) using 16S rRNA gene sequencing. Comparisons between Field Control (FC) and short-term Captive Treatment (CT) groups revealed a marked reduction in microbial diversity under captive conditions. Observed richness decreased approximately five-fold (Field Control: 1026 features; Captive Treatment: 221 features), and Shannon diversity declined from 8.89 to 5.93. Beta diversity analyses based on UniFrac distances demonstrated clear separation between groups, indicating substantial shifts in community composition. Taxonomic profiling revealed reduced community complexity in captive fish, with increased dominance of Proteobacteria and loss of diverse environmental taxa. Predicted enrichment of pathways associated with stress response, altered respiration, and metabolic flexibility in captivity reflects inferred functional potential rather than direct functional activity. Given the use of pooled samples with limited biological replication, these findings should be interpreted as strong community-level patterns rather than population-level inference. Collectively, these results indicate that short-term captivity alters the F. heteroclitus gut microbiome. Full article

Increasing pressure on surface water resources in intensive agricultural regions has driven the search for sustainable alternatives for irrigation supply, especially in areas where water quality limits crop safety and export opportunities. In this context, riverbank filtration (RBF) systems offer a nature-based solution by utilizing physical, chemical, and biological processes associated with river–aquifer exchange. However, their implementation depends on suitable site selection supported by hydrogeological, geomorphological, and hydraulic criteria. This study developed an integrated methodology to identify zones with potential for implementing RBF systems in the Roldanillo–Unión–Toro irrigation district, located in northern Valle del Cauca, Colombia. This region requires irrigation water over 10,256 ha of agricultural land (mainly sugarcane, maize, grapes, and guava). We combined geophysical methods (vertical electrical soundings, 2D electrical resistivity tomography, and passive seismic), geotechnical methods (CPTu tests), and hydraulic characterization of the river reach to evaluate subsurface stratigraphy, preliminary hydrogeological suitability, inferred river–aquifer connectivity conditions, and channel stability. The evaluation covered four sectors along an approximately 21 km stretch of the Cauca River’s left-bank alluvial valley. The results revealed pronounced lateral and vertical heterogeneity of alluvial materials. However, the “El Palmar” sector was identified as the best-supported priority sector for future RBF validation, due to the presence of profile-scale evidence of potentially permeable sandy and gravelly units with intermediate resistivity values (52–61 Ω·m), favorable stratigraphic organization, and stable river-reach conditions during the field campaign. In contrast, the other three sectors (La Esperanza, Candelaria, and Cayetana) showed more fine-grained sediments with deeper permeable strata. River-flow measurements during the July 2025 field campaign indicated high discharge conditions at the evaluated reach, while river-channel observations showed active fine-sediment transport; these findings provide hydraulic and sedimentary context for the future evaluation of induced infiltration and potential clogging, but do not constitute direct evidence of river–aquifer exchange. This study highlights the value of integrated screening approaches for prioritizing candidate RBF sites in agricultural alluvial settings, while indicating that pumping tests, piezometric monitoring, hydraulic-gradient analysis, and water-quality validation remain necessary before engineering implementation. Full article

This study proposes a rigorous methodology for the optimal placement of voltage regulators in the Guayacanes feeder of the Buena Fe substation, Ecuador, by integrating electrical feeder modeling, exhaustive search, and multi-criteria decision-making. The feeder was modeled in detail by incorporating its radial topology, nodal electrical parameters, and representative operating conditions under minimum- and maximum-load scenarios. Based on this model, a set of technical evaluation criteria was established to quantify the impact of regulator installation, including active power losses, reactive power losses, global voltage deviation, average voltage variation, and voltage imbalance. An exhaustive search strategy was then implemented to evaluate all feasible regulator-location alternatives over the candidate nodes, thereby ensuring a complete exploration of the solution space. The resulting alternatives were ranked using the Weighted Sum Method (WSM) and the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS), allowing the comparison of candidate locations from a multi-criteria perspective. The results indicate that node MTS 108932 provides the most technically favorable overall solution, achieving the greatest improvement in voltage profile quality and the most significant reduction in electrical losses. In addition, a sensitivity analysis was conducted by varying the weighting structure of the decision criteria, confirming the robustness of the selected alternative under different decision-maker preference scenarios. The proposed framework provides a technically sound decision-support methodology for voltage regulation planning in real radial distribution systems. Full article

Meat from culled cows is characterized by increased toughness and limited processing suitability, yet the combined effects of microbial cultures and mechanical processing have not been sufficiently studied. The aim of this study was to evaluate the effect of Lactobacillus sakei (SafePro B-2, 10¹¹ CFU/g) in combination with vacuum massage on the physicochemical, structural, and functional–technological properties of culled cow meat, as well as to optimize the process using response surface methodology. It was found that the combined treatment led to a 25–35% reduction in shear force and a 12–18% increase in water-holding capacity compared to control samples. The pH value decreased from 6.2 to 5.6–5.8, indicating active lactic acid fermentation. Modeling using the response surface method demonstrated high predictive power of the models (R² = 0.91–0.96) and allowed for the determination of optimal process parameters (10⁷ CFU/g; 40 min of mixing), ensuring maximum softening and product stability. Analysis of the texture profile revealed a statistically significant (p < 0.05) decrease in hardness, stickiness, and chewiness, accompanied by an increase in cohesiveness, indicating a reorganization of the protein matrix at the microstructural level. Additionally, microstructural modifications and reorganization of the protein matrix were observed, accompanied by changes in amino acid composition, indicating a change in the state of proteins and their spatial distribution within the meat matrix. Overall, the combined use of Lactobacillus sakei and vacuum massaging effectively improves the functional, technological, and structural characteristics of low-grade beef raw materials. The proposed approach can be considered a scientifically sound strategy for optimizing processing parameters and increasing the efficiency of utilizing meat from culled cows. Full article

Background: Long COVID, or post-COVID-19 condition (PCC), affects around 36% of individuals following SARS-CoV-2 infection, manifesting as persistent fatigue, cognitive dysfunction, and dysautonomia among its hallmark features. Affecting an estimated 400 million individuals globally, it imposes an annual economic burden exceeding $1 trillion, yet no pharmacological therapy has demonstrated consistent efficacy in adequately powered randomized controlled trials. Transcutaneous auricular vagus nerve stimulation (taVNS) has emerged as a candidate intervention targeting the autonomic dysfunction and neuroinflammation responsible for PCC pathophysiology. Methods: We conducted a PRISMA 2020-compliant systematic review (PROSPERO: CRD420261287286) searching PubMed, Scopus, Cochrane, and Web of Science databases from inception to January 2026 for studies evaluating any form of VNS in adults with Long COVID. Risk of bias was assessed using the Cochrane Risk of Bias 2 (RoB 2) tool, the JADAD scale, and the PEDro scale. Certainty of evidence was evaluated using the GRADE framework. Narrative synthesis followed SWiM guidelines. Results: Five studies (n = 154 participants) (three randomized controlled trials (RCTs) and two single-arm studies) met inclusion criteria. Three of five studies (60%) were rated high overall risk of bias; only two RCTs achieved “some concerns.” The only adequately double-blinded RCT found no significant between-group differences across all outcomes. Paradoxically, in the best-powered RCT (Percin et al.), sham stimulation produced significantly greater fatigue improvement than active taVNS, despite active taVNS producing significant HRV increases consistent with cardiac autonomic modulation. All efficacy outcomes were rated “very low” certainty (GRADE); safety was rated “low” certainty. Conclusions: Currently available evidence supporting the use of taVNS for Long COVID remains limited, and the absence of reliable target engagement markers in the included studies constrains confidence in this approach. Nonetheless, the physiological rationale remains sound, and the favorable safety profile across all included studies supports the feasibility of future investigation. However, given that positive findings were confined to inadequately controlled studies, enthusiasm for further research should be directed first toward mechanistic clarification and rigorous dose-finding work. Large-scale, double-blind, sham-controlled trials incorporating validated markers of vagal engagement are required before taVNS can be firmly recommended for COVID-19 sequelae management. Full article

Background: Active middle ear implants (AMEIs) provide an alternative auditory rehabilitation strategy for patients who cannot tolerate conventional hearing aids. However, clinical data regarding the outcomes of Vibrant Soundbridge (VSB) implantation using the incus short process (SP) coupler in older adults remain limited. Objective: This study aimed to evaluate the audiological outcomes, patient-reported hearing benefits, tinnitus improvement, and surgical safety of VSB implantation using the SP coupler in older adults with bilateral sloping sensorineural hearing loss. Methods: This retrospective study included 45 older male veterans (mean age 76.1 ± 5.3 years) with bilateral sloping sensorineural hearing loss who underwent unilateral VSB implantation with the SP coupler between 2019 and 2023. Functional hearing gain was assessed using preoperative and postoperative sound-field pure-tone thresholds. Patient-reported outcomes were evaluated using the Speech, Spatial and Qualities of Hearing Scale (SSQ) and the Tinnitus Handicap Inventory (THI). Operative characteristics and postoperative complications were also analyzed. Results: Mean operative time was 40.2 ± 8.7 min. Functional hearing gain increased progressively across speech-critical frequencies, reaching +20 dB at 2 kHz and +30 dB at 4 kHz. The mean four-frequency pure tone average improved from 57.4 ± 8.3 dB HL preoperatively to 35.6 ± 6.9 dB HL postoperatively (p < 0.001). All SSQ subdomains showed significant improvement (p < 0.001). THI scores decreased significantly from 43.2 ± 8.4 to 17.1 ± 6.2 (p < 0.0001), with clinically meaningful tinnitus improvement observed in 75.6% of patients. No major surgical complications occurred. Conclusions: Vibrant Soundbridge implantation using the incus short process coupler provides effective auditory rehabilitation for older adults with sloping sensorineural hearing loss. The procedure yields meaningful high-frequency hearing gain, improved hearing-related quality of life, and significant tinnitus reduction while maintaining a favorable surgical safety profile. Restoration of auditory input through active middle ear implantation may also contribute to improved central auditory processing in older adults. Full article

Sikkim, located in the northeastern Himalaya, is highly vulnerable to natural hazards and increasing depletion of surface and subsurface water resources, particularly springs and lakes. In South Sikkim, several lakes exhibit rapid drainage behavior, among which Nagi Lake shows near-complete water loss shortly after rainfall, indicating the presence of subsurface leakage pathways. This study investigates shallow subsurface moisture dynamics and identifies potential seepage-prone zones beneath the Nagi Lake basin using geoelectrical methods. Electrical resistivity profiling was conducted along seven survey lines during the non-rainy season (October–November 2025) to minimize the influence of transient rainfall-induced moisture variations. Profiling was carried out using the Wenner method, achieving investigation depths of approximately 6.5–9 m. Additionally, Vertical Electrical Sounding (VES) using the Schlumberger configuration was performed at selected locations to examine deeper subsurface conditions. Resistivity results indicate that profiles L1, L2, L3, L4, and L7 contain relatively higher moisture restricted to the upper ~5 m, whereas profiles L5 and L6 exhibit persistently low resistivity values from the surface to depths of ~9 m, suggesting sustained subsurface moisture accumulation. The downward extension of low-resistivity zones along L5 and L6 indicates possible preferential seepage pathways or localized subsurface water storage. VES results further reveal a higher density of subsurface anomalies below ~14 m in these areas. These low-resistivity anomalies are interpreted as potential subsurface flow pathways. Although confirmation of active seepage requires additional hydrological or time-lapse investigations, the findings provide important baseline geophysical insights for lake rejuvenation. Full article

Validated instruments assessing gender stereotype endorsement among adolescents are scarce and often overlook contemporary domains like digital privacy. To address this gap, this study developed and validated the Gender Stereotypes and Roles Adherence Battery for Adolescents (GAB-A) in a sample of 2955 Italian adolescents attending public secondary schools in Rome (56.4% male; mean age 14.3 years). The battery comprises three modules: the Gender Stereotyped Attitude Scale (GSAS), Gender Role Activities Scale (GRAS), and Gendered Traits Inventory (GTI). Psychometric analysis confirmed robust factor structures, notably identifying a distinct “Relational Control” factor within the GSAS that assesses beliefs normalizing partner surveillance. The results revealed a stark pattern of gender differentiation: males endorsed prescriptive attitudes (GSAS, d = 1.07) and roles (GRAS, d = 0.88) substantially more than females, particularly regarding violence myths. Conversely, essentialist trait beliefs (GTI) showed negligible gender differences (d = 0.11). Associations between stereotypes and psychological health were gender-moderated; within-group analyses indicated that endorsement predicted higher distress, hostility, and alexithymia in males, while being unrelated to well-being in females. Finally, gender-stratified normative data and operational cut-offs were established. The GAB-A provides a psychometrically sound tool for identifying elevated endorsement profiles and evaluating violence prevention interventions. Full article

Passive acoustic monitoring (PAM) is a valuable tool for ecological research, but many eco-acoustic indices show inconsistent correlations with biodiversity due to methodological variability and environmental noise. We propose a complementary, physically interpretable approach using energy-derived metrics. We analyzed audio recordings from three sites near a major highway in the Ticino River Park (Milan, Italy) using 1 sec equivalent continuous sound pressure level (Leq_1s), peak interval statistics, maximal-overlap discrete-wavelet transform (MODWT), and temporal fractal analysis. This multi-resolution type of approach enabled frequency-specific tracking of acoustic energy and temporal structure. Our results reveal site-specific differences: Site 3, the most distant from the highway, showed higher high-frequency energy and longer temporal persistence, suggesting richer biophonic activity. Site 1, the closest to the highway, displayed flatter spectral profiles and faster autocorrelation decay. Diel patterns were reflected in hourly Leq trends, while fractal analysis revealed frequency- and site-dependent acoustic memory. These automated findings were corroborated by expert annotations of bird activity and traffic. The integration of Leq_1s, peak metrics, and wavelet decomposition offers a suitable framework for soundscape characterization, with strong potential for long-term ecoacoustic monitoring and habitat quality assessment in complex environments. Full article

Phonoenterography (PEG) offers a non-invasive and radiation-free technique to assess gastrointestinal activity through acoustic signal analysis. In this feasibility study, 110 high-resolution PEG recordings (44.1 kHz, 16-bit) were acquired from eight healthy individuals, yielding 6314 prominent bowel sound (PBS) segments through automated segmentation. Each event was characterized using a 279-feature acoustic profile comprising Mel-frequency cepstral coefficients (MFCCs), their first-order derivatives (Δ-MFCCs), and six global spectral parameters. After normalization and dimensionality reduction with PCA and UMAP (cosine distance, 35 neighbors, minimum distance = 0.01), five clustering strategies were evaluated. K-Means (k = 5) achieved the most favorable balance between cluster quality (silhouette = 0.60; Calinski–Harabasz = 19,165; Davies–Bouldin = 0.68) and interpretability, consistently identifying five acoustic patterns: single-burst, multiple-burst, harmonic, random-continuous, and multi-modal. Temporal modeling of clustered events further revealed distinct sequential dynamics, with Single-Burst events showing the longest dwell times, random continuous the shortest, and strong diagonal elements in the transition matrix confirming measurable state persistence. Frequent transitions between random continuous and multi-modal states suggested dynamic exchanges between transient and overlapping motility patterns. Together, these findings demonstrate that unsupervised PEG-based analysis can capture both acoustic variability and temporal organization of bowel sounds. This annotation-free approach provides a scalable framework for real-time gastrointestinal monitoring and holds potential for clinical translation in conditions such as postoperative ileus, bowel obstruction, irritable bowel syndrome, and inflammatory bowel disease. Full article

Despite recent progress in lymphoma immunotherapy, outcomes for patients with peripheral T cell lymphomas (PTCLs) remain poor. The challenge of PTCLs reflects the profound biological heterogeneity and relative rarity of this disease group and its resistance to conventional chemotherapy, as well as the formidable challenge of generating definitive clinical evidence. However, deepening insight into the immunogenomic and microenvironmental basis of PTCL has revealed diverse mechanisms of immune escape, spanning defects in antigen presentation, apoptotic signaling, adhesion, and extensive tumor microenvironmental remodeling. These vulnerabilities provide a sound rationale for novel immunotherapeutic strategies—checkpoint inhibitors, CAR-T and NK cell platforms, bispecific antibodies, oncolytic viruses, and immunomodulatory agents. Early studies show encouraging but inconsistent activity, and the variability in response highlights the urgent need for biomarker-driven stratification to deliver personalized approaches and clinically meaningful efficacy. This review synthesizes the current literature on the immune dysregulation of PTCLs, as well as advances in PTCL immunotherapy, outlining the biological rationale underpinning these approaches. We discuss approaches to molecular, transcriptomic, and microenvironmental profiling with circulating biomarkers that could enable adaptive trial designs and personalized treatment strategies. Together, these developments chart a path away from empiricism and toward precision therapy in PTCLs. Full article

To address the problems of low efficiency and poor real-time performance in underwater acoustic modeling, as well as the requirement of maximizing search coverage for underwater target search path planning, this paper proposed an efficient path planning method based on Sound Speed Profile (SSP) clustering. Firstly, the SSPs were dimensionally reduced via Empirical Orthogonal Function (EOF) decomposition, and the sea area was divided into 10 acoustic sub-areas using K-means clustering after fusing geographic coordinates and terrain information, thereby constructing a block-wise sound field model. Secondly, with the active sonar equation as the core, sonar parameters such as the noise level and target strength were solved, respectively, to generate a spatial distribution matrix of search distances. Finally, an Improved Ant Colony Optimization (IACO) algorithm was modified by dynamically setting the pheromone evaporation rate and improving the heuristic information for search path optimization. Numerical experiments showed that clustering significantly improves the efficiency of sound field modeling, reducing the time consumption of the transmission loss calculation from 24.74 h to 10.84 min. The IACO increased the average search coverage from 47.96% to 86.01%, with an improvement of 79.34%. The performance of IACO is superior to those of the compared algorithms, providing support for efficient underwater target search. Full article

Valorization of Phoenix dactylifera L. (date) seeds, an abundant agro-industrial byproduct, offer a sustainable approach to developing multifunctional ingredients for dermocosmetic photoprotection. Rich in polyphenols, flavonoids, and lipophilic antioxidants, date seed extracts and oils demonstrate promising UV-absorbing, anti-inflammatory, and free-radical-scavenging properties. Recent in vitro, ex vivo, and preclinical studies underscore their potential as bioactive agents in sunscreen formulations, supporting both skin barrier integrity and oxidative stress mitigation, although clinical validation is still required. This review consolidates current knowledge on the phytochemical profile and biological efficacy of date seed derivatives, with emphasis on their integration into advanced delivery systems such as nanocarriers, Pickering emulsions, and cyclodextrin complexes to enhance photostability, skin permeability, and esthetic acceptability. Safety aspects, including allergenicity, phototoxicity, and regulatory gaps, are critically examined alongside environmental and ethical advantages, including biodegradability and vegan suitability. The findings advocate for the inclusion of Phoenix dactylifera L. seed actives in next-generation dermocosmetic sunscreens that align with circular bioeconomy principles, consumer demand for “reef-safe” products, and evolving international regulations. Further clinical validation is encouraged to fully translate these botanically derived agents into effective and ethically sound sun care innovations. Full article

Harmful algal blooms (HABs), caused by the dinoflagellate Alexandrium pacificum, are increasingly frequent in the Marlborough Sounds, an important aquaculture region in Aotearoa New Zealand. Alexandrium pacificum produces paralytic shellfish toxins and blooms cause significant economic and ecological disruptions through contamination of edible shellfish. High-throughput sequencing of prokaryotic and eukaryotic communities was used to investigate community dynamics during bloom events across two consecutive summers. Distinct successional shifts were observed, with prokaryotic communities dominated by Rhodobacterales and Flavobacteriales during blooms, and increased abundance of the SAR11 clade (Pelagibacterales) post-bloom. Eukaryotic diversity was dominated by Alexandrium species (Gonyaulacales) during the bloom, and subsequently shifted towards Syndiniales, Gymnodiniales, and Peridiniales as blooms collapsed. Significant correlations indicated potential ecological roles for these taxa in bloom regulation, particularly Syndiniales, which could indicate parasitic interactions. Depth profiles revealed consistent microbial composition throughout the water column, validating depth-integrated sampling strategies for community studies. This research describes changes in the composition of microbial communities during two A. pacificum blooms, suggesting that species interactions (e.g., via parasitism) may play a role shaping bloom dynamics. Further studies incorporating environmental parameters, especially nutrient dynamics linked to anthropogenic activities, are necessary to better understand the drivers of blooms in this important aquaculture region. Full article