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Compliance checks are a critical aspect of any agricultural production and supply chain. These comprise a chain of activities and responsibilities shared by producers and regulators. Currently, the responsibility for collecting and processing data primarily lies with the regulators. Regulators are the primary users of regulatory technologies, while producers, such as farmers, have limited capabilities. This has been due to a lack of reliable hardware and software technology that can be deployed at the producer’s sites. However, recent advancements in Artificial Intelligence (AI), the Industrial Internet of Things (IIoT), and cloud computing have significantly increased reliability and reduced deployment costs. This paper reviews the regulatory landscape of agriculture, regulatory technologies, IIoT, and their feasibility for producers, using a new Technology Readiness Framework for Compliance, specifically designed for Regulatory Technologies (RegTech) and regulation compliance. It classifies technologies into three categories: Early Stage, Emerging, and Established. It concludes that most essential agricultural issues already have mature technological solutions within the scope of IIoT that producers can use directly without supervision, while still maintaining the integrity and validity of the data. The paper discusses and measures the maturity of IIoT and other electronic and digital technologies for integration into RegTech. This categorization offers a new perspective on technology readiness and lays the foundation for future RegTech platform design, e.g., by decentralizing and empowering producers with reliable technologies. Full article

Fresh market blueberry (Vaccinium spp.) fruits are fragile and experience numerous impacts during harvest, packing, and shipping. Mechanical harvest of southern highbush blueberries (SHB) is being increasingly implemented due to rising costs and limited availability of labor. As new commercial cultivars become available, questions arise among growers as to their suitability for mechanical harvest. Early spring harvests in growing areas in the southeastern U.S. routinely occur when ambient temperatures exceed 30 °C. A series of experiments was conducted over a decade to determine the effects of mechanical impacts on fruit quality. These experiments employed a 60 cm drop height to induce bruising under three scenarios encountered during commercial harvest and handling. (1) Harvest interval: Nonimpacted ‘Star’ and ‘Sweetcrisp’ fruits had higher soluble solids content to titratable acidity ratios (SSC:TA) after a 7-day interval (Harvest 2) as compared with those from the initial Harvest 1. Impacted ‘Star’ blueberries from Harvest 2 were 70–100% softer during 14-d storage at 1 °C/85% relative humidity than those from Harvest 1, whereas ‘Sweetcrisp’ fruits were less affected by the harvest delay (30–40% increase in soft fruit). (2) Pulp temperature at impact: There were no differences in bruise severity for ‘Meadowlark’, ‘Colossus’, or ‘Sentinel’ due to pulp temperature at impact. Overall, impacted fruits consistently exhibited greater weight loss (3% to 9%), were softer, and had more severe bruising compared with nonimpacted controls. (3) Delays between harvest and impact: Delay-to-impact (5 or 24 h) did not affect weight loss for ‘Meadowlark’ (0.57% to 0.62%) during 4 d of storage at 5 °C. ‘Colossus’ and ‘Sentinel’, held overnight at 22 °C, lost approximately 35% to 45% more fresh weight after the 24 h delay to impact compared with those fruits with the 5 h delay to impact. Impacted blueberries exhibited significantly more severe bruising (38.5% to 84.4%) than control fruits (1.0% to 8.3%). ‘Sentinel’ was softer at harvest than the other cultivars and had the highest amount of severe bruising (82.7%), followed by ‘Meadowlark’ (52.67%) and ‘Colossus’ (42.57%). Flavor profiles varied by cultivar, with SSC:TA ratios ranging from 18 (‘Colossus’) to 21 (‘Meadowlark’) to 44 (‘Sentinel’). Immediately after impact at 15 °C, 20 °C, or 30 °C, the respiration rate (RR) for ‘Meadowlark’ increased as compared with the control fruit. RR for fruits at 5 °C or 10 °C remained fairly constant during the 8 h measurement period. These findings highlight the interactions of harvest interval, pulp temperature, and delay to impact on the postharvest quality of several commercially grown, SHB cultivars over this extended period of time. These three factors must be considered in order to develop effective strategies for mechanical harvest under the warm spring conditions encountered in the subtropical growing conditions in the southeastern U.S.A. Full article

Pepino (Solanum muricatum Aiton) is highly susceptible to Alternaria solani-induced early blight, a well-studied pathogen in other Solanaceae but rarely investigated in pepino. Although branching critically shapes plant architecture and environmental adaptability, its relationship with disease resistance remains unclear. Field trials compared natural growth (W1) and manual bud removal (W2); W2 showed a disease incidence of 51.0% ± 4.8, significantly lower than that of W1 (64% ± 4.8), and a reduced disease index (DI) of 3.79 ± 1.46 at 30 days after treatment. Pot experiments evaluated three plant growth regulators (PGRs): flumetralin (TA), pendimethalin (TB), and butralin (TC). All suppressed lateral buds, with TA most effective—achieving a 75.25% ± 1.23 bud suppression rate (BSR) and 61.00% ± 1.46 bud suppression efficacy (BSE), along with shorter plant height (16.0 ± 1.4 cm), thicker stems (7.43 mm ± 0.29), and larger leaves (12.39 cm² ± 0.73) compared to the control. Under A. solani stress, PGR-treated plants exhibited markedly enhanced resistance, as evidenced by smaller lesion areas, elevated superoxide dismutase (SOD) and peroxidase (POD) activity, reduced malondialdehyde (MDA), and increased defense hormones—especially salicylic acid (SA) and indole-3-acetic acid (IAA). TA boosted SA and IAA by 2.25× and 2.35× compared to the control. These findings demonstrate that PGRs mediated bud suppression not only optimizes plant architecture but also strengthens antioxidant and hormonal defenses, offering a sustainable strategy for pepino production. Full article

TaC/amorphous-carbon (TaC/a-C) composite coatings with varied a-C contents were deposited on graphite by dual-target magnetron sputtering to mitigate the thermal-expansion mismatch that commonly triggers cracking and spallation in TaC coatings on carbon substrates during rapid thermal cycling. However, existing TaC–C (often termed “free carbon”) approaches rarely identify the carbon’s structural state and spatial distribution explicitly, and a clear correlation between carbon fraction, thermal-shock-driven microstructural evolution, and cyclic damage remains insufficiently established. Increasing the a-C fraction progressively refines the TaC grain structure and introduces an a-C phase along grain boundaries, thereby lowering the effective coefficient of thermal expansion (CTE) and improving compatibility with the graphite substrate. Under laser thermal cycling, coatings with higher a-C contents exhibit markedly enhanced resistance to cracking and spallation. After 15 cycles, the high-a-C (~28.99 at.%) coating remains free of through-thickness cracks, maintains its thickness, and retains a single-phase TaC structure without detectable Ta₂C, whereas the low-a-C coating shows severe thinning, through-cracks, and partial TaC → Ta₂C transformation. Microstructural observations indicate that the a-C phase forms a compliant, stress-relaxing boundary network and promotes a porous, mechanically interlocked TaC architecture, synergistically redistributing thermal stresses and deflecting crack propagation. Full article

This study investigates the efficacy of co-assembled, physically cross-linked nanocarriers comprising tannic acid (TA) and a P(DMAEMA-co-OEGMA) random/statistical double-hydrophilic copolymer for ovalbumin (OVA) encapsulation. TA-based nanocarriers, prepared at varying TA molar ratios (10% w/v and 20% w/v), exhibited nanoaggregates of different sizes, as revealed by dynamic light scattering, with Nanocarrier 1 system showing populations of 11 and 109 nm, while Nanocarrier 2 formed a single population of 75 nm in size. Notably, both colloidal systems demonstrated stability under thermal treatment and resilience to changes in salt concentrations higher than 0.15 M, but disassembly phenomena in basic media. Utilizing these nanocarriers for OVA loading via electrostatic interactions revealed strong positive charges (~30 mV) for all protein-loaded nanocarrier cases. In particular, they demonstrated sizes within the desired range (R_h = 96–118 nm) and considerable stability over 20 days and in the presence of serum proteins. Overall, this study underscores the importance of physical cross-linking as a viable strategy for the formation of tunable nanometric hydrocolloids for effective protein encapsulation, with significant implications for drug delivery systems. Full article

Neural interfaces created using thin-film fabrication rely primarily on conductive metal traces for electrical interconnects. Here, we explore the use of tantalum (Ta) metal interconnects as a replacement for noble-metal interconnects such as Au, Pt or Ir. Ta has been investigated previously for interconnect metallization in flexible silicon ribbon cables, but the structure and properties of tantalum for neural device metallization have not been extensively reported. In the present work, Ta metal was sputter-deposited onto amorphous silicon carbide (a-SiC), with and without a base titanium (Ti) adhesion layer, and investigated as interconnect metallization. In the absence of a Ti adhesion layer, resistivity measurements revealed a factor of six difference between Ta resistivity depending on the presence of the Ti base layer, with direct deposition on a-SiC nucleating high resistivity β-Ta (ρ = 197 ± 31 µΩ·cm, mean ± standard deviation) and Ta deposited on Ti nucleating low resistivity α-Ta (ρ = 35 ± 6 µΩ·cm). X-ray diffraction confirmed the existence of the two crystal structures. Ta feature sizes of 2 µm were created using photolithography and reactive ion etching (RIE). Finally, planar microelectrode array test structures using α-Ta and Au trace metallization with low-impedance ruthenium oxide (RuO_x) electrodes were fabricated and investigated by cyclic voltammetry (CV) and current pulsing in saline. These devices underwent 500 CV cycles between −0.6 and +0.6 V without evidence of degradation. In response to charge-balanced, biphasic current pulses at 4 nC/phase, a 21 mV increase in access voltage was observed with α-Ta metallization compared to Au. These results warrant further investigation of Ta as thin-film metallization interconnects for neural interface devices. Full article

Breast cancer is a leading cause of cancer-related mortality in women, necessitating new treatment strategies. Curcumin (Cur), a natural polyphenol, and gemcitabine (Gem), a standard chemotherapeutic, were investigated for their combined anticancer effects. We hypothesized that Cur sensitizes breast cancer cells to Gem via reactive oxygen species (ROS)-mediated apoptosis, and that this effect is associated with selective oxidative vulnerability in malignant cells compared to normal breast epithelial cells. MCF-7 (hormone receptor-positive) and MDA-MB-231 (triple-negative) cells were treated with Cur and Gem alone or in combination. Normal breast epithelial MCF-10A cells were included to evaluate therapeutic selectivity. Cell viability (MTT), apoptosis (Annexin V/PI), oxidative stress (TOS/TAS), intracellular ROS generation (DCFH-DA assay), mitochondrial membrane potential (ΔΨm) (JC-1 staining), caspase activation, synergy (Bliss/HSA/Chou-Talalay), VEGF secretion (ELISA), and transcriptomic changes (RNA-Seq) were assessed. Cur and Gem showed dose-dependent cytotoxicity. Combination treatment demonstrated strong synergistic activity, significantly enhancing apoptosis, oxidative stress, and caspase activation. Direct quantification of intracellular ROS revealed marked ROS accumulation in MCF-7 and MDA-MB-231 cells following combination treatment, whereas MCF-10A cells exhibited only modest oxidative changes. JC-1 analysis demonstrated substantial mitochondrial depolarization in breast cancer cells, which was largely reversible by ROS scavenging and minimal in MCF-10A cells. VEGF secretion was markedly suppressed. Transcriptomic analysis revealed profound alterations in apoptosis, cell cycle, and angiogenesis-related pathways, with more pronounced transcriptional reprogramming observed in the triple-negative subtype. Cur synergistically enhances Gem’s efficacy in breast cancer cells through ROS-mediated apoptosis and anti-angiogenic effects, characterized by cancer-selective ROS amplification and mitochondrial membrane depolarization, supporting its potential as a combination therapy, particularly for triple-negative breast cancer. Full article

A multiple strong coupling system comprising monolayer MoS₂ and Ag nanodisk (Ag-ND) arrays is investigated using transient absorption (TA) spectroscopy. By tuning the diameter and period of the Ag-NDs arrays, the surface plasmon polariton (SPP) resonances are made to simultaneously overlap with the A (~660 nm) and B (~608 nm) excitons of monolayer MoS₂. As a result, three distinct negative ground-state bleaching (GSB) peaks, corresponding to the upper (UP), middle (MP), and lower (LP) hybrid polariton states, were observed in the TA spectra. This confirms that a multiple strong coupling regime was achieved with both the A and B excitons of monolayer MoS₂ and SPPs modes, which was also highlighted by the anti-crossing behavior across varied Ag-NDs arrays parameters. Finally, by adding an insulating spacer layer of Al₂O₃ film, the coupling strength can be modulated from a strong coupling regime to a weak coupling regime. These results reveal a multi-exciton–plasmon strong coupling system and establish a versatile platform for ultrathin polaritonic devices, including polariton lasers and all-optical switches. Full article

The Mexican Tropical Pacific (MTP) is a key component of the Eastern Tropical North Pacific Oxygen Minimum Zone, yet its carbonate system variability remains poorly constrained. This study examines wind-driven circulation effects on dissolved oxygen (DO) and the carbonate system —dissolved inorganic carbon (DIC), total alkalinity (TA), total-scale pH (pH_T), partial pressure of CO₂ in seawater (pCO_2w) and air–sea CO₂ fluxes (FCO₂)— in the Gulf of Tehuantepec (GT) and Tehuantepec Bowl (TB). Hydrographic data and discrete water samples were collected at 50 oceanographic stations during March 2020. Principal Component Analysis (PCA) identifies wind-driven circulation as the primary control of biogeochemical variability. Tehuano wind events and mesoscale eddies promoted upwelling of low-oxygen (DO < 20 µmol kg⁻¹) and high-DIC (>2200 µmol kg⁻¹) waters to 50 m depth in the central GT, while downwelling conditions prevailed in the TB. Stoichiometric analysis revealed DIC-DO coupling (slope = −1.39). Overall, the MTP acted as CO₂ source (FCO₂ ranging from −1.92 to 24.11 mmol m⁻² d⁻¹), with enhanced emissions linked to eddy-induced upwelling. This study provides the first integrated characterization of the carbonate system across both the GT and TB. Full article

The Nanping pegmatite-type Nb-Ta deposit is one of the large-scale Li-Cs-Ta (LCT)-type pegmatite deposits in Southeast China. Nevertheless, the mineralization mechanism of this ore deposit remains unclear, primarily due to the lack of systematic research on the characteristics of ore-forming fluids and mineralization processes. To address this issue, analyses of the fluid inclusion characteristics, hydrogen–oxygen isotope compositions and in situ U-Pb geochronology of Nb-Ta minerals were performed on the No. 31 vein of the Nanping pegmatite deposit. In situ U-Pb dating of the Nb-Ta minerals with varying textures from different zones yields main mineralization ages clustered between 390 and 370 Ma, along with isolated younger ages around 270 Ma in specific mineral zones, indicating multiple mineralization episodes. The fluid inclusion homogenization temperatures of different zones range from 130 to 382 °C, and salinities between 2 and 16 wt% NaCl eqv, consistent with a medium-to-low temperature and salinity fluid system. Hydrogen and oxygen isotope data show that the ore-forming fluids were predominantly derived from magmatic fluids, mixed with later meteoric waters. This study clarifies the multistage mineralization history and fluid evolution of the Nanping pegmatite-type Nb-Ta deposit, providing key constraints for metallogenic models of pegmatite-hosted rare-metal deposits. Full article

This study explored a range of cooling interventions suitable for city laneways where space for greening opportunities is constrained. Five individual cooling interventions namely, PVC shading, cool pavement, small canopy trees, green wall and water mist, as well as multiple combinations of these individual cooling interventions were tested in a narrow laneway in the temperate setting of Melbourne, Australia. The impact of various cooling interventions was assessed by evaluating microclimatic parameters—air temperature (T_a), relative humidity (RH), mean radiant temperature (T_MRT)—alongside two thermal comfort indices, Physiological Equivalent Temperature (PET) and Universal Thermal Climate Index (UTCI). When each intervention was analysed individually, water mist was the best performing with T_a, PET and UTCI reduction. This was followed by PVC shading, small canopy trees and green walls. Cool pavement had the lowest T_a reduction and minimal thermal comfort impact. While green provided marginal reductions in thermal comfort indices, the effects were insufficient for standalone cooling. They were most effective when integrated with other cooling interventions. For example, when green walls were combined with water mist, a T_a reduction of 1.49 K and a T_MRT reduction 2.57 K were obtained. The water mist system as an individual cooling intervention or as part of a combined intervention had an impact on T_a with a reduction of maximum 1.3 K and 1.76 K, respectively. The water mist had a UTCI reduction of 1.25 K, and the water mist combined with green wall had a PET reduction of 1.84 K. The novel contribution of this study to climate-sensitive urban design is the suite of practical, site-specific interventions for extreme summer conditions. These findings provide a framework for planners and designers to evaluate and implement optimal cooling strategies tailored to the unique microclimate demands of narrow urban laneways. Full article

Background/Objectives: Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) is a global health crisis, but current diagnostics are limited. Liver biopsy is invasive, magnetic resonance imaging-proton density fat fraction (MRI-PDFF) is expensive, and quantitative ultrasound methods are low-accuracy, especially in patients with a high body mass index (BMI). This study introduces a novel thermo-acoustic (TA) method that generates ultrasound signals based on tissue electrical conductivity, where lean tissue (high in water and electrolytes) absorbs more radio-frequency (RF) energy than fatty tissue, providing a direct molecular contrast for fat. Methods: A prospective, cross-sectional feasibility study compared a new thermo-acoustic fat fraction (TAFF) score with the reference standard MRI-PDFF in 40 subjects with suspected fatty liver disease. Bland–Altman analysis, Deming regression, and Binary classification performance were tested. To establish system stability, a dedicated Repeatability and Reproducibility (R&R) study (N = 14) evaluated inter-operator and intra-operator consistency using an Intraclass Correlation Coefficient (ICC) derived from a two-way random-effects ANOVA model. Results: TAFF estimates demonstrated a substantial correlation (r = 0.89) with MRI-PDFF and an average absolute error of 3.04% fat fraction. Classification performance was high, with an Area Under the Receiver Operating Characteristic Curve (AUROC) of 0.92 at the 12% fat fraction threshold and 0.99 at the 20% fat fraction threshold. The R&R study confirmed robust stability (intraclass correlation = 0.89) and a negligible mean inter-operator difference of 0.36%. Estimation errors showed no statistically significant correlation with BMI or other body habitus measurements. Conclusions: These findings support thermoacoustics’ potential as an accurate, non-invasive, point-of-care solution that can serve as a new imaging biomarker. By providing predictive values closely aligned with MRI-PDFF across the full MASLD spectrum, TAFF can complement currently available ultrasound methods to address the cost and access constraints of MRI for the assessment, diagnosis, and monitoring of MASLD. Full article

Multi-material structures based on titanium alloys represent a promising approach for the fabrication of functionally graded orthopedic implants capable of combining high mechanical strength with reduced stiffness to minimize the stress-shielding effect. In the present work, multi-material Ti15Ta/Ti6Al4V specimens were fabricated by laser powder bed fusion (L-PBF) for the first time, and the processing parameters of the transition zone were systematically optimized. Three regimes were investigated: baseline (93 J/mm³), double scanning (186 J/mm³), and reduced speed (116 J/mm³). The microstructure and elemental distribution were examined by SEM and EDS; mechanical properties were evaluated through tensile testing and microhardness measurements; biocompatibility was assessed using osteoblasts and gingival fibroblasts. The double scanning regime provided the highest density of the transition zone (99.49%). Tensile failure of the specimens occurred in the Ti15Ta region, confirming the quality of the metallurgical bond. The ultimate tensile strength ranged from 534 to 543 MPa with an elongation at break of 15.7–16.4%. Heat treatment at 875 °C led to the formation of an equilibrium lamellar microstructure and smoothing of the interface. Cell viability on both alloys exceeded 88% as confirmed by flow cytometry and remained above the 70% non-cytotoxicity threshold defined by ISO 10993-5. The obtained results demonstrate the technological feasibility of fabricating multi-material Ti15Ta/Ti6Al4V structures and achieving high-quality metallurgical bonding, which constitutes a necessary first step toward the development of functionally graded orthopedic implants. Full article

The brewing process generates substantial by-products rich in potentially bioactive compounds (e.g., polyphenols and fermentation metabolites), providing a sustainable and appealing source of cosmetic ingredients. Oil-in-water (O/W) emulsions containing 20% (w/w) aqueous extracts from Bionda Triplo Malto beer, wort, and key brewing by-products (hops, yeast, and spent grain) were developed and evaluated using a combined in vitro–in vivo approach. Aqueous extracts were first screened on human immortalized dermal fibroblasts (BJ-5ta) at 0.25–1 mg/mL for cytocompatibility and antioxidant activity. Within this concentration range, no significant changes in cell viability or intracellular antioxidant capacity under UV stress were detected, suggesting cytocompatibility but limited inherent activity. When incorporated into O/W emulsions and tested at an active-equivalent concentration of 10 mg/mL, the formulations increased fibroblast metabolic activity and antioxidant response. In contrast, free extracts at 10 mg/mL showed concentration-dependent cytotoxicity for some matrices, with beer- and yeast-based emulsions demonstrating the strongest effects. The emulsions exhibited good physicochemical stability (pH ~5.7–6.2; viscosity 4750–5150 mPa·s), passed the ISO 11930:2012 challenge test, and were well tolerated in patch testing. In a double-blind, randomized split-forearm study on 50 healthy volunteers over 30 days, beer, yeast, and spent grain-based formulations improved skin parameters versus baseline. TEWL decreased (e.g., beer: 16.22 ± 5.12 to 10.77 ± 2.22 mg·m⁻²·h⁻¹; yeast: 16.29 ± 5.66 to 10.18 ± 1.08; spent grain: 14.45 ± 4.34 to 11.66 ± 2.28), hydration increased (beer: 35.15 ± 5.93 to 42.26 ± 3.78; yeast: 33.27 ± 4.87 to 42.92 ± 2.48; spent grain: 34.22 ± 5.19 to 41.16 ± 3.17, and elasticity improved for beer and yeast formulations (62.33 ± 3.27 to 70.24 ± 2.12 N/m) and yeast (61.21 ± 4.72 to 72.13 ± 5.55 N/m). Based on these findings, brewing-derived ingredients demonstrate potential as cosmetic actives, with formulation critically determining their clinical efficacy. Full article

The non-repeatability of the internal combustion engine’s cycle-by-cycle (CCN-R) operation directly affects pollutant emissions, fuel consumption, and energy efficiency. Reducing this non-repeatability is an important part of efforts to improve the environmental performance of power units. Cycle variability analysis allows the identification of engine operating areas that promote unstable combustion and increased emissions of harmful exhaust components. The aim of the study was to quantitatively assess the cycle-to-cycle non-repeatability COV of selected operating parameters of the Perkins 1104D-E44TA diesel engine. The analyses covered the maximum cylinder pressure (p_max), the mean indicated pressure (IMEP), and the crankshaft rotation angle corresponding to the occurrence of maximum pressure (α). The measurements were carried out on an engine dynamometer at 25 operating points, covering speeds 1000–2200 r./min and load torques 200–400 N × m, recording 500 consecutive operating cycles at each point. The results showed that the most stable engine operation occurred at medium rotational speeds and moderate loads, where COV_pmax values did not exceed 0.5% and COV_IMEP values were lower than 1.0%. Increased pmax non-repeatability (up to 2.10%) and very high α angle variability (up to 100–140%) were observed at high rotational speeds and high loads. Only in the case of COV_IMEP was a significant reduction in repeatability observed compared to idling. The results obtained from cycle-by-cycle non-repeatability analyses can ultimately, after being supplemented with exhaust gas composition testing, be used as tools to support engine control optimization in order to reduce pollutant emissions and improve combustion efficiency. Full article