Search Results (22)

This paper presents a wideband pattern-reconfigurable antenna designed for 360° horizontal sensing with angle-of-arrival (AoA) estimation capability. The antenna features a unique three-layer planar architecture, where a microstrip circular array is integrated between two metallic plates to enhance radiation stability and bandwidth. By employing a single-pole four-throw (SP4T) switching circuit, the array generates four steerable beams covering the entire azimuthal plane. Experimental results show that the prototype achieves a 10 dB return loss impedance bandwidth of 50% (4.0–6.0 GHz) and a peak gain of 8.3 dBi. Based on this antenna, a correlation-coefficient-based AoA estimation approach is implemented. The measured results demonstrate reliable estimation performance, with a mean angular error of less than 1.5° over the 360° horizontal plane across the operating frequency range. The proposed design provides a compact and low-complexity solution for practical wideband direction-finding applications in next-generation wireless systems. Full article

As adults, some newts exhibit a multiphasic lifestyle, switching between aquatic and terrestrial habitats. Under laboratory conditions, we provided an experiment to expose newts, which were in their aquatic phase, to air as a surrounding medium. We studied how the body temperature changed when the ambient temperature increased. The thirteen specimens used in the study belonged to two species from the genus Triturus sp.—T. ivanbureschi and T. dobrogicus. For temperature measurements, we used a precise thermometer with a beaded thermocouple and an IR thermal camera. We started the experiment at 17 °C and increased the air temperature by approximately 1 °C every 10 min. The newts were exposed to the air until the first signs of physical exhaustion appeared. An increase of 1 °C in ambient temperature led to an average increase of 0.87 °C in the body temperature of the newts across the four experimental days. The measured body temperature showed a consistent increase during all experimental sessions, but it did not equalize with the environmental temperature. The body temperature in all specimens remained lower by an average of 2.24 (±0.02) °C. Full article

Switched filter banks find widespread application in frequency-hopping radar systems and communication networks with multiple operating frequencies, especially in situations demanding elevated filter element isolation. In this paper, the design and implementation of a highly isolated switchable narrow-bandpass filter bank architecture using hairpin microstrip topology is presented. The filter bank has four discrete bandpass filters with passbands of 2.0–2.2 GHz, 2.3–2.5 GHz, 3.1–3.3 GHz, and 3.9–4.1 GHz. These filters span the radar S-frequency band (2.0–4.0 GHz). In order to switch between channels with a switching speed of nanoseconds, low-loss and highly isolated SP4T switches are implemented. Advanced design system (ADS) software is used to design the various filter functionalities, and the entire system is tested on a vector network analyzer (VNA). The proposed architecture makes it much easier to put the filter bank into practice and switch it to the desired frequency, which is useful for radar receiver applications. Full article

This review summarizes current advances in the role of transcriptional stochasticity in HIV-1 latency, which were possible in a large part due to the development of single-cell approaches. HIV-1 transcription proceeds in bursts of RNA production, which stem from the stochastic switching of the viral promoter between ON and OFF states. This switching is caused by random binding dynamics of transcription factors and nucleosomes to the viral promoter and occurs at several time scales from minutes to hours. Transcriptional bursts are mainly controlled by the core transcription factors TBP, SP1 and NF-κb, the chromatin status of the viral promoter and RNA polymerase II pausing. In particular, spontaneous variability in the promoter chromatin creates heterogeneity in the response to activators such as TNF-α, which is then amplified by the Tat feedback loop to generate high and low viral transcriptional states. This phenomenon is likely at the basis of the partial and stochastic response of latent T cells from HIV-1 patients to latency-reversing agents, which is a barrier for the development of shock-and-kill strategies of viral eradication. A detailed understanding of the transcriptional stochasticity of HIV-1 and the possibility to precisely model this phenomenon will be important assets to develop more effective therapeutic strategies. Full article

This paper designs a five-bit microelectromechanical system (MEMS) time delay consisting of a single-pole six-throw (SP6T) RF switch and a coplanar waveguide (CPW) microstrip line. The focus is on the switch upper electrode design, power divider design, transmission line corner compensation structure design, CPW loading U-shaped slit structure design, and system simulation. The switch adopts a triangular upper electrode structure to reduce the cantilever beam equivalent elastic coefficient and the closed contact area to achieve low drive voltage and high isolation. The SP6T RF MEMS switch uses a disc-type power divider to achieve consistent RF performance across the output ports. When designed by loading U-shaped slit on transmission lines and step-compensated tangents at corners, the system loss is reduced, and the delay amount is improved. In addition, the overall size of the device is 2.1 mm × 2.4 mm × 0.5 mm, simulation results show that the device has a delay amount of 0–60 ps in the frequency range of 26.5–40 GHz, the delay accuracy at the center frequency is better than 0.63 ps, the delay error in the whole frequency band is less than 22.2%, the maximum insertion loss is 3.69 dB, and the input–output return rejection is better than 21.54 dB. Full article

A moderately thermophilic Gram-positive chemo-organotrophic bacterium, strain SP2, was isolated by serial dilutions with crotonate and yeast extract as substrates from a butyrate-degrading methanogenic enrichment obtained from thermophilically digested sludge of the Kuryanoskaya wastewater treatment plant (Moscow, Russia). Cells of strain SP2 are spore-forming rods, sometimes occurring in short chains. The bacterium is an obligate anaerobe that grows at temperatures from 20 to 70 °C (55–60 °C optimum) within a pH range of 3.5–8 (7.5 optimum) and with NaCl concentrations of up to 2.5%. The strain utilized yeast extract and simple sugars as carbon and energy sources. Thiosulfate was used as an electron acceptor when grown on sucrose, resulting in the formation of hydrogen sulfide and the accumulation of elemental sulfur globules inside the cells. Strain SP2 is phylogenetically related to Biomaibacter acetigenes strain SK-G1^T as revealed by comparison with the 16S rRNA gene (99.9% identity) and genome (ANI 99%, dDDH 90%) of both strains. It is interesting that strain SP2 was capable of syntrophic conversion of glycerol and lactate when co-cultivated with hydrogenotrophic methanogen, which was not previously shown for the SK-G1^T type of strain. The isolation and in-depth study of new facultatively syntrophic microorganisms is important for wastewater treatment ecotechnologies due to their ability to switch to an alternative source of carbon and energy and therefore greater resistance to changing environmental conditions in bioreactors. Full article

A frequency-modulated continuous-wave radar for short-range target imaging, assembling a transceiver, a PLL, an SP4T switch, and a serial patch antenna array, was realized. A new algorithm based on a double Fourier transform (2D-FT) was developed and compared with the delay and sum (DAS) and multiple signal classification (MUSIC) algorithms proposed in the literature for target detection. The three reconstruction algorithms were applied to simulated canonical cases evidencing radar resolutions close to the theoretical ones. The proposed 2D-FT algorithm exhibits an angle of view greater than 25° and is five times faster than DAS and 20 times faster than the MUSIC one. The realized radar shows a range resolution of 55 cm and an angular resolution of 14° and is able to correctly identify the positions of single and multiple targets in realistic scenarios, with errors lower than 20 cm. Full article

With the rapid development of the Internet and wireless communications, as well as the popularization of personal communication systems, the security of real-time communications is demanded. The efficient technology of stream ciphers can satisfy this requirement of security. In this paper, to enhance the security strength of stream ciphers, we design a key-based multi-mode clock-controlled stream cipher for real-time secure communications of the Internet of things (IoT). The proposed stream cipher is equipped with a multi-mode depending on the key. The different working modes are shipped with different encrypting circuits depending on the user’s key. We analyze the period, the linear complexity, and use known attacks to verify the security strength of the proposed cipher. Compared with existing dual mode clock-controlled stream ciphers, the merits of our proposed cipher are its long period, high linear complexity, low hardware complex, low initialization clock, and simplicity in mode switching. Furthermore, the proposed cipher passes the FIPS PUB 140-1 and SP800-22 tests, obtaining at least 97.00%. Full article

This paper describes a 2.4-GHz fully-integrated front-end receiver including a single-pole triple-throw (SP3T) switch and a low-noise amplifier (LNA) with bypass function, which was fabricated in a 0.25 μm GaAs pHEMT process. An asymmetrical SP3T switch architecture is incorporated to enable the receiver to operate in four modes. The exploration of impedance and voltage gain behavior of the proposed LNA help to establish the matching network and alleviate the trade-off between noise figure (NF) and gain performance. In LNA high gain mode, the implemented front-end receiver shows 1.7 dB of NF and 6dBm of input third-order intercept point (IIP3) with 20 dB of power gain drawing 11 mA of current from 5 V power supply at 2.4 GHz. All input and output return loss had exceeded 10 dB with fully on-chip impedance matching network. In bypass mode, the measured insertion loss of typically 7.5 dB is achieved. Full article

Eriophyoid mites of the genus Trisetacus Keifer are widespread parasites of conifers. A new oligophagous species, T. indelis n. sp., was discovered severely damaging seeds of North American junipers (Juniperus osteosperma, J. occidentalis, and J. californica) in the western USA. It has two codon deletions in the mitochondrial gene Cox1 rarely detected in Eriophyoidea and includes distinct morphological dimorphism of females. A phylogenetic analysis based on amino acid alignment of translated Cox1 sequences using a large set of out-groups (a) determined that two North American congeners, T. batonrougei and T. neoquadrisetus, were the closest known relatives of T. indelis n. sp., and (b) indicated that Old and New World seed-inhabiting Trisetacus from junipers do not form a distinct clade, suggesting a possible independent transition to living in seeds of junipers in America and Eurasia by Trisetacus spp. Our analysis produced a new topology consistent with a scenario assuming gradual reduction of prodorsal shield setation in Eriophyoidea and an ancient switch from gymnosperms to other hosts. Additionally, our analysis did not support monophyly of Trisetacus; recovered a new host-specific, moderately supported clade comprising Trisetacus and Nalepellinae (Nalepella + Setoptus) associated with Pinaceae; and questioned the monophyly of Trisetacus associated with Cupressaceae. Full article

(1) Background: Recent data shed light on the association between atopic disorders (ADs) (atopic dermatitis, allergic asthma, allergic rhinitis) and spondyloarthropathies (SpAs), underpinning the critical role of T helper (Th)1-Th17/Th2-T regulatory cells disbalance. We evaluated the prevalence of AD in axial SpAs (axSpAs) and psoriatic arthritis (PsA) and explored the potential association between atopic status, disease-related parameters, and biological therapy. (2) Methods: A monocentric, retrospective study was conducted that enrolled 200 patients taking biologics. Demographics, disease, and drug-related variables, along with a screening questionnaire focused on Ads, were systematically collected. (3) Results: Overall, 51 patients (25.5%) had atopy—namely, 24.4% of axSpA and 28% of PsA, with a higher frequency of rhinitis (43%) vs. atopic dermatitis (37.2%) or asthma (21.5%). We failed to demonstrate any statistically significant difference in demographics, SpA-related parameters excepting concomitant inflammatory bowel disease, and biologic drug exposure in patients with and without atopy (p > 0.05). However, significantly more non-atopic patients need only one TNF inhibitor (54%) vs. atopic patients (28%) (p < 0.05) to control active SpA. (4) Conclusions: We successfully demonstrated that AD is associated with one out of four SpA. Irrespective of the SpA subtype, atopic patients require more frequent switching among biologics, as significantly more non-atopic patients remain on their first anti-TNF. Full article

In the present paper, we extend the current literature in algorithmic trading with Markov-switching models with generalized autoregressive conditional heteroskedastic (MS-GARCH) models. We performed this by using asymmetric log-likelihood functions (LLF) and variance models. From 2 January 2004 to 19 March 2021, we simulated 36 institutional investor’s portfolios. These used homogenous (either symmetric or asymmetric) Gaussian, Student’s t-distribution, or generalized error distribution (GED) and (symmetric or asymmetric) GARCH variance models. By including the impact of stock trading fees and taxes, we found that an institutional investor could outperform the S&P 500 stock index (SP500) if they used the suggested trading algorithm with symmetric homogeneous GED LLF and an asymmetric E-GARCH variance model. The trading algorithm had a simple rule, that is, to invest in the SP500 if the forecast probability of being in a calm or normal regime at t + 1 is higher than 50%. With this configuration in the MS-GARCH model, the simulated portfolios achieved a 324.43% accumulated return, of which the algorithm generated 168.48%. Our results contribute to the discussion on using MS-GARCH models in algorithmic trading with a combination of either symmetric or asymmetric pdfs and variance models. Full article

The photovoltaic (PV) system center inverter architecture comprises various conventional array topologies such as simple-series (S-S), parallel (P), series-parallel (S-P), total-cross-tied (T-C-T), bridge-linked (B-L), and honey-comb (H-C). The conventional PV array topologies under non-uniform operating conditions (NUOCs) produce a higher amount of mismatching power loss and represent multiple maximum-power-points (M-P-Ps) in the output characteristics. The performance of T-C-T topology is found superior among the conventional topologies under NUOCs. However, T-C-T topology’s main limitations are higher redundancy, more number of electrical connections, higher cabling loss, poor performance during row-wise shading patterns, and more number of switches and sensors for the re-configuration of PV modules. This paper proposes the various optimal hybrid PV array topologies to overcome the limitations of conventional T-C-T array topology. The proposed hybrid topologies are such as series-parallel-cross-tied (S-P-C-T), bridge-link-cross-tied (B-L-C-T), honey-comb-cross-tied (H-C-C-T), series-parallel-total-cross-tied (S-P-T-C-T), bridge-link-total-cross-tied (B-L-T-C-T), honey-comb-total-cross-tied (H-C-T-C-T), and bridge-link-honey-comb (B-L-H-C). The proposed hybrid topologies performance is evaluated and compared with the conventional topologies under various NUOCs. The parameters used for the comparative study are open-circuit voltage, short-circuit current, global-maximum-power-point (GMPP), local-maximum-power-point (LMPP), number of LMPPs, and fill factor (FF). Furthermore, the mismatched power loss and the conversion efficiency of conventional and hybrid array topologies are also determined. Based on the results, it is found that the hybrid array topologies maximize the power output by mitigating the effect of NUOCs and reducing the number of LMPPs. Full article

Biodegradation of 1,4-dioxane (dioxane) contamination has gained much attention for decades. In our previous work, we isolated a highly efficient dioxane degrader, Xanthobacter sp. YN2, but the underlying mechanisms of its extraordinary degradation performance remained unresolved. In this study, we performed a comparative transcriptome analysis of YN2 grown on dioxane and citrate to elucidate its genetic degradation mechanism and investigated the transcriptomes of different dioxane degradation stages (T0, T24, T48). We also analyzed the transcriptional response of YN2 over time during which the carbon source switched from citrate to dioxane. The results indicate that strain YN2 was a methylotroph, which provides YN2 a major advantage as a pollutant degrader. A large number of genes involved in dioxane metabolism were constitutively expressed prior to dioxane exposure. Multiple genes related to the catabolism of each intermediate were upregulated by treatment in response to dioxane. Glyoxylate metabolism was essential during dioxane degradation by YN2, and the key intermediate glyoxylate was metabolized through three routes: glyoxylate carboligase pathway, malate synthase pathway, and anaplerotic ethylmalonyl–CoA pathway. Genes related to quorum sensing and transporters were significantly upregulated during the early stages of degradation (T0, T24) prior to dioxane depletion, while the expression of genes encoding two-component systems was significantly increased at late degradation stages (T48) when total organic carbon in the culture was exhausted. This study is the first to report the participation of genes encoding glyoxalase, as well as methylotrophic genes xoxF and mox, in dioxane metabolism. The present study reveals multiple genetic and transcriptional strategies used by YN2 to rapidly increase biomass during growth on dioxane, achieve high degradation efficiency and tolerance, and adapt to dioxane exposure quickly, which provides useful information regarding the molecular basis for efficient dioxane biodegradation. Full article

A bacteriophage switches between lytic and lysogenic life cycles. The AimR-AimP-AimX communication system is responsible for phage lysis-lysogeny decisions during the infection of Bacillus subtilis. AimX is a regulator biasing phage lysis, AimR is a transcription factor activating AimX expression, and AimP is an arbitrium peptide that determines phage lysogeny by deactivating AimR. A strain-specific mechanism for the lysis-lysogeny decisions is proposed in SPbeta and phi3T phages. That is, the arbitrium peptide of the SPbeta phage stabilizes the SPbeta AimR (spAimR) dimer, whereas the phi3T-derived peptide disassembles the phi3T AimR (phAimR) dimer into a monomer. Here, we find that phAimR does not undergo dimer-to-monomer conversion upon arbitrium peptide binding. Gel-filtration, static light scattering (SLS) and analytical ultracentrifugation (AUC) results show that phAimR is dimeric regardless of the presence of arbitrium peptide. Small-angle X-ray scattering (SAXS) reveals that the arbitrium peptide binding makes an extended dimeric conformation. Single-molecule fluorescence resonance energy transfer (smFRET) analysis reveals that the phAimR dimer fluctuates among two distinct conformational states, and each preexisting state is selectively recognized by the arbitrium peptide or the target DNA, respectively. Collectively, our biophysical characterization of the phAimR dynamics underlying specific target recognition provides new mechanistic insights into understanding lysis-lysogeny decisions in Bacillus phage phi3T. Full article