Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Article Types

Countries / Regions

Search Results (93)

Search Parameters:
Keywords = SKinTES

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
18 pages, 570 KiB  
Article
Exploring the Bioavailability of Red Grape Skin Extract Polyphenols: A Caco-2 Cell Model Study
by Edurne Elejalde, Rosa María Alonso, María Carmen Villarán, Lucía Díez-Gutiérrez, María Chávarri and Iratxe López-de-Armentia
Foods 2025, 14(13), 2253; https://doi.org/10.3390/foods14132253 - 25 Jun 2025
Viewed by 390
Abstract
Grapes are a rich source of polyphenols with a positive impact on human health. Polyphenols need to be bioavailable to exert any beneficial effect. However, there is limited knowledge on the bioavailability of polyphenols in grape extracts. The intestinal permeability of nine polyphenols [...] Read more.
Grapes are a rich source of polyphenols with a positive impact on human health. Polyphenols need to be bioavailable to exert any beneficial effect. However, there is limited knowledge on the bioavailability of polyphenols in grape extracts. The intestinal permeability of nine polyphenols of a red grape skin extract (GSE) was investigated using the Caco-2 cell model that simulates the human intestinal epithelium: three anthocyanins (delphinidin-3-O-glucoside, petunidin-3-O-glucoside and malvidin-3-O-glucoside), three flavonols (quercetin-3-glucoside, kaempferol-3-galactoside and kaempferol-3-glucoside), two hydroxybenzoic acids (gallic acid and syringic acid) and one hydroxycinnamic acid (caftaric acid). Two concentrations of GSE (15 mg/mL and 22 mg/mL) were used. The transport efficiency (TE) through the Caco-2 monolayer was studied. Among anthocyanins, only malvidin-3-O-glucoside was detected at the basolateral side, which represents the bloodstream, with a TE of 1.08 ± 0.01%. Flavonols resulted in a variety of results depending on the GSE concentration. Among flavonols, kaempferol-3-glucoside showed the highest TE of 130 ± 3%. Gallic acid showed the highest TE among the investigated polyphenols with 188 ± 3%. This study provides data on the intestinal transport of red grape skin extract polyphenols that can be used to explore the underlying mechanisms of the intestinal absorption and the bioactivity of natural grape extracts. Full article
Show Figures

Graphical abstract

12 pages, 918 KiB  
Article
TiO2 Nanoparticles Loaded with Polygonum cuspidatum Extract for Wound Healing Applications: Exploring Their Hemolytic, Antioxidant, Cytotoxic, and Antimicrobial Properties
by Gabriela Fletes-Vargas, Rogelio Rodríguez-Rodríguez, Natalha Vicentina Pinto, Kelly Cristina Kato, Guilherme Carneiro, Ana Paula Rodrigues, Helen Rodrigues-Martins and Hugo Espinosa-Andrews
Nanomaterials 2025, 15(12), 926; https://doi.org/10.3390/nano15120926 - 14 Jun 2025
Cited by 1 | Viewed by 544
Abstract
The dry roots of Polygonum cuspidatum contain resveratrol, a compound known for its antimicrobial and protective effects against oxidative stress, which is associated with impaired wound healing. In this study, titanium dioxide nanoparticles (TiO2NPs) were loaded with a P. cuspidatum extract [...] Read more.
The dry roots of Polygonum cuspidatum contain resveratrol, a compound known for its antimicrobial and protective effects against oxidative stress, which is associated with impaired wound healing. In this study, titanium dioxide nanoparticles (TiO2NPs) were loaded with a P. cuspidatum extract (TiO2-loaded extract NPs), and the resveratrol release profile, hemocompatibility, antioxidant, cytotoxic, and antimicrobial activities were evaluated. The results demonstrated that TiO2-loaded extract NPs exhibited antioxidant activity for DPPH (Inhibitory Concentration 50 (IC50) = 62.31 mg Trolox Equivalent (TE)/mL) and ABTS+ (IC50 = 4.8 mg TE/mL) assays, along with suitable hemocompatibility (3.02% at 10 mg/mL), in comparison with bulk TiO2 NPs. Additionally, temperature influenced the resveratrol release over time. The P. cuspidatum extract alone showed strong antibacterial activity, with a Minimal Inhibitory Concentration (MIC) of 5 µg/mL, TiO2-loaded extract NPs showed MIC values about 50 mg/mL, while bulk TiO2 NPs exhibited no antibacterial effect against the tested strains. In contrast, the P. cuspidatum extract, the TiO2-loaded extract NPs, and the bulk TiO2 NPs did not demonstrate antifungal activity against Candida albicans and C. glabrata. Moreover, TiO2-loaded extract NPs showed no cytotoxicity against the L-929 cell line at concentrations ranging from 1.5 to 150 µg/mL, unlike TiO2 NPs, which exhibited high cytotoxic concentrations between 9.4 and 300 µg/mL. These findings suggest that TiO2-loaded extract NPs effectively control the release of resveratrol and hold promises for applications in skin management and wound healing. Full article
(This article belongs to the Special Issue Applications of Functional Nanomaterials in Biomedical Science)
Show Figures

Figure 1

14 pages, 792 KiB  
Article
Cryofibrinogenemia in PRECOVID-19 and COVID-19 Periods: Single University Study in Northern Spain
by María del Amparo Sánchez López, Carmen Lasa-Teja, Mónica Renuncio-García, Cristina Abraira-Meriel, Saray Simón-Coloret, Inmaculada Bertomeu-Genis, Adrián Martín-Gutiérrez, Carmen Secada-Gómez, Carmen González-Vela, Juan Irure-Ventura, Marcos López-Hoyos, Marcos A. González-López and Ricardo Blanco
Sci 2025, 7(2), 81; https://doi.org/10.3390/sci7020081 - 6 Jun 2025
Viewed by 345
Abstract
Cryofibrinogenemia (CF) may be secondary to COVID-19. To establish this relationship, in PRECOVID-19 and COVID-19 periods we assess: (a) frequency and clinical features in patients with CF; (b) study of CF syndrome. We study all cryofibrinogen tests performed in a single university hospital [...] Read more.
Cryofibrinogenemia (CF) may be secondary to COVID-19. To establish this relationship, in PRECOVID-19 and COVID-19 periods we assess: (a) frequency and clinical features in patients with CF; (b) study of CF syndrome. We study all cryofibrinogen tests performed in a single university hospital in Northern Spain, comparing two periods: PRECOVID-19 (July 2017–February 2020) and COVID-19 (March 2020–October 2022). CF syndrome was established with two positive cryofibrinogen tests plus compatible cutaneous manifestations and/or thrombotic events (TE). CF was found in 129/279 patients. In the COVID-19 period, they had more positive tests (50.2% vs. 28%; p = 0.0047), younger age (33 vs. 55 years, p = 0.054) and fewer cardiovascular (CV) risk factors (39.1% vs. 78.6%, p = 0.005). Cutaneous manifestations were the most frequent in both periods (81.4%), particularly purpuric macules (29.5%). Skin ulcers showed statistically significant differences, being more frequent in the PRECOVID-19 era (35.7% vs. 7.8%, p = 0.008). Thrombotic CV events were also observed (13.2%), particularly venous thromboembolisms (12.2%). Severe complications were more frequent in the PRECOVID-19 era, although this difference did not reach statistical significance (35.7% vs. 19.1%; p = 0.169). CF was secondary in 68/129 cases, mainly to SARS-CoV-2 (n = 45). CF syndrome was found in 27.9% of patients. After one year, most patients were clinically stable or in remission. Mild dermatological lesions were the most frequent manifestations, and most patients recovered. Full article
(This article belongs to the Section Biology Research and Life Sciences)
Show Figures

Figure 1

10 pages, 944 KiB  
Article
Morphometric Measurements Prior to Totally Endoscopic Mitral Valve Repair: Technical and Educational Aspects
by Marie-Elisabeth Stelzmueller, Daniel Zimpfer and Wilfried Wisser
J. Clin. Med. 2025, 14(8), 2581; https://doi.org/10.3390/jcm14082581 - 9 Apr 2025
Viewed by 520
Abstract
Objective: The totally endoscopic approach is on the rise to become the new standard in mitral valve surgery. The aim of this study was to develop a morphometric measurement tool for educational purposes to predict operability with low conversion and high repair [...] Read more.
Objective: The totally endoscopic approach is on the rise to become the new standard in mitral valve surgery. The aim of this study was to develop a morphometric measurement tool for educational purposes to predict operability with low conversion and high repair rates. Methods: From January 2020 to March 2023, 64 patients underwent totally endoscopic mitral valve repair (TE-MVR). Of these, 15 patients were deemed to be unsuitable for TE-MVR due to narrow space and/or anticipated complex repair techniques and underwent repair through sternotomy (MVR-open). Angio-CT scanning was performed for preoperative planning and measurements of the following: the distance between the sternum and the spine (DSS), the distance between the skin incision and the anterior anulus of the mitral valve (DNM) and the intercostal space at the level of the skin incision (ICS). Results: The repair rate for all patients was 98.7%. In the TE-MVR group, the conversion rate to sternotomy was 3.1%. The 30-day survival was 100%. The DSS was 130.4 ± 18.8 mm and 108.1 ± 17.3 mm, and the DSM 70.7 ± 12.1 mm and 58.5 ± 13.6 mm in the TE-MVR and MVR-open, respectively (p < 0.001). Twenty-one TE-MVR patients were found to be technically demanding due to friction and less freedom to move the instruments. The composite morphometric parameter DSS plus 4xICS minus DNM was 53.3, 39.8 and 25.6 for TE-TMReasy, TE-TMRdemanding and MVR-open, respectively (p < 0.05 and p < 0.01). Conclusions: Surgical skills and a long history of expertise are mandatory to achieve excellent results with a low conversion and high repair rate. The composite morphometric parameter may be an easy tool for educational demands to predict the ease and feasibility of TE-MVR. Full article
(This article belongs to the Special Issue Mitral Valve Surgery: Current Status and Future Challenges)
Show Figures

Figure 1

41 pages, 28708 KiB  
Article
Identification and Characterization of LINE and SINE Retrotransposons in the African Hedgehog (Atelerix albiventris, Erinaceidae) and Their Association with 3D Genome Organization and Gene Expression
by Mengyuan Zhu, Jianxuan Zhou, Nannan Chen, Jianing Xu, Haipeng Wang, Libo Jiang and Fengtang Yang
Genes 2025, 16(4), 397; https://doi.org/10.3390/genes16040397 - 29 Mar 2025
Viewed by 935
Abstract
Background: The African hedgehog (Atelerix albiventris) exhibits specialized skin differentiation leading to spine formation, yet its regulatory mechanisms remain unclear. Transposable elements (TEs), particularly LINEs (long interspersed nuclear elements) and SINEs (short interspersed nuclear elements), are known to influence genome organization [...] Read more.
Background: The African hedgehog (Atelerix albiventris) exhibits specialized skin differentiation leading to spine formation, yet its regulatory mechanisms remain unclear. Transposable elements (TEs), particularly LINEs (long interspersed nuclear elements) and SINEs (short interspersed nuclear elements), are known to influence genome organization and gene regulation. Objectives: Given the high proportion of SINEs in the hedgehog genome, this study aims to characterize the distribution, evolutionary dynamics, and potential regulatory roles of LINEs and SINEs, focusing on their associations with chromatin architecture, DNA methylation, and gene expression. Methods: We analyzed LINE and SINE distribution using HiFi sequencing and classified TE families through phylogenetic reconstruction. Hi-C data were used to explore TE interactions with chromatin architecture, while whole-genome 5mCpG methylation was inferred from PacBio HiFi reads of muscle tissue using a deep-learning-based approach. RNA-seq data from skin tissues were analyzed to assess TE expression and potential associations with genes linked to spine development. Results: SINEs form distinct genomic blocks in GC-rich and highly methylated regions, whereas LINEs are enriched in AT-rich, hypomethylated regions. LINEs and SINEs are associated differently with A/B compartments, with SINEs in euchromatin and LINEs in heterochromatin. Methylation analysis suggests that younger TEs tend to have higher methylation levels, and expression analysis indicates that some differentially expressed TEs may be linked to genes involved in epidermal and skeletal development. Conclusions: This study provides a genome-wide perspective on LINE and SINE distribution, methylation patterns, and potential regulatory roles in A. albiventris. While not establishing a direct causal link, the findings suggest that TEs may influence gene expression associated with spine development, offering a basis for future functional studies. Full article
(This article belongs to the Section Animal Genetics and Genomics)
Show Figures

Figure 1

8 pages, 2005 KiB  
Proceeding Paper
Numerical Analysis of Potential Energy Recovery via a Thermoelectric Generator (TEG) for the Next-Generation Hybrid-Electric Regional Aircraft
by Safa Sabet, Werner Gumprich, Michael Moeller, Andrés Felgueroso, Iván González Nieves, Miguel Díaz and Simone Mancin
Eng. Proc. 2025, 90(1), 64; https://doi.org/10.3390/engproc2025090064 - 18 Mar 2025
Viewed by 334
Abstract
The thermal management of next-generation hybrid electric regional aircrafts poses critical challenges due to extreme heat loads, which could reach more than 2 MW and must be dissipated. This rejected heat can be used in a passive system such as Thermoelectric Generators (TEGs), [...] Read more.
The thermal management of next-generation hybrid electric regional aircrafts poses critical challenges due to extreme heat loads, which could reach more than 2 MW and must be dissipated. This rejected heat can be used in a passive system such as Thermoelectric Generators (TEGs), which can directly convert thermal energy into electrical energy. This work is carried out in the framework of the EU Clean Aviation-funded project TheMa4HERA and it numerically explores the possibility of integrating thermoelectric (TE) technology in the next generation of regional aircrafts. Two case studies are considered: energy recovery from the outflow valve originally used to control the pressure of the cabin and the integration of TEG modules in skin heat exchangers used to partially dissipate heat coming from the fuel cells and/or from the power electronics. The results will permit us to understand the feasibility of implementing TEG technology into these specific conditions in terms of overall power generation. The findings indicate that while TEG integration in the outflow valve offers limited power density, the skin heat exchanger shows significantly higher potential for effective energy recovery. Full article
Show Figures

Figure 1

15 pages, 4913 KiB  
Article
Effect of Fibroblast Growth Factor-2 on Melanocyte Proliferation in Tissue-Engineered Skin Substitutes
by Karel Ferland, Brice Magne, Henri De Koninck, Martin A. Barbier, Danielle Larouche and Lucie Germain
Int. J. Mol. Sci. 2025, 26(4), 1704; https://doi.org/10.3390/ijms26041704 - 17 Feb 2025
Viewed by 858
Abstract
Burn patients treated with tissue-engineered skin substitutes (TESs) often experience pigmentation irregularities, including hypopigmentation and pigmentation spots. These issues are thought to stem from the reduced presence of melanocytes through dilution during TES manufacturing. To address this, we hypothesized that supplementing epithelial cell [...] Read more.
Burn patients treated with tissue-engineered skin substitutes (TESs) often experience pigmentation irregularities, including hypopigmentation and pigmentation spots. These issues are thought to stem from the reduced presence of melanocytes through dilution during TES manufacturing. To address this, we hypothesized that supplementing epithelial cell cultures—primarily composed of keratinocytes but also containing melanocytes—with Fibroblast Growth Factor-2 (FGF-2), a known promoter of melanocyte proliferation, could enhance melanocyte growth. This would potentially increase their numbers in TESs and improve pigmentation outcomes. Our findings indicate that FGF-2, at an optimal dose of 0.2 nM, effectively maintains melanocyte numbers in 2D cultures and epithelial cell cultures through the first passage. Importantly, this treatment does not interfere with keratinocyte proliferation or differentiation, nor does it affect TES integrity. However, FGF-2 supplementation alone did not increase the proportion of melanocytes in epithelial cultures beyond the first passage or in TESs. In summary, while FGF-2 supports melanocyte growth in culture, its addition alone was insufficient to significantly improve TES pigmentation. Full article
(This article belongs to the Special Issue Pigment Cells: From Biology to Medicine)
Show Figures

Figure 1

28 pages, 905 KiB  
Article
Exploring the Phytochemical Profile and Biological Insights of Epilobium angustifolium L. Herb
by Reneta Gevrenova, Gokhan Zengin, Gulsah Ozturk and Dimitrina Zheleva-Dimitrova
Plants 2025, 14(3), 415; https://doi.org/10.3390/plants14030415 - 31 Jan 2025
Cited by 3 | Viewed by 1110
Abstract
The aerial parts of Epilobium angustifolium L. (fireweed) (Onagraceae) are renowned for their use in the treatment of prostatic, kidney and urinary tract diseases, and skin infections. In this work, a comprehensive phytochemical profiling of the methanol-aqueous extract from E. anfustifolium aerial parts [...] Read more.
The aerial parts of Epilobium angustifolium L. (fireweed) (Onagraceae) are renowned for their use in the treatment of prostatic, kidney and urinary tract diseases, and skin infections. In this work, a comprehensive phytochemical profiling of the methanol-aqueous extract from E. anfustifolium aerial parts was performed by the means of liquid chromatography–Orbitrap high-resolution mass spectrometry. Annotation and dereplication of 121 secondary metabolites were achieved, including acylquinic acids, gallo- and ellagitannins, flavonoids, phenolic acids, and their glycosides. Forty-six compounds are reported for the first time in the species. Total phenolic and flavonoid content were 85.04 ± 0.18 mg GAE/g and 27.71 ± 0.74 mg QE/g, respectively. Antioxidant capacity assessment revealed that the extract actively scavenged DPPH and ABTS radicals (310.74 and 466.82 mg TE/g) along with a high reducing power in CUPRAC and FRAP assay (442.83 and 291.50 mg TE/g), respectively, and metal chelating (48.20 mg EDTA/g). The extract also had a distinct impact on α-glucosidase (3.48 mmol ACAE/g) and moderate activity towards α-amylase (0.44 mmol ACAE/g) and lipase (8.03 OE/g). It inhibited acetyl- and butyrylcholinesterase (2.05 and 1.67 mg GALE/g) and had a prominent anti-tyrosinase effect (61.91 mg KA/g). Our results contribute to establishing fireweed as a multifunctional agent for use in herbal preparations. Full article
(This article belongs to the Section Phytochemistry)
Show Figures

Figure 1

21 pages, 10391 KiB  
Article
NADES-in-Oil Emulsions Enriched with Essential Oils for Cosmetic Application
by David S. Freitas, Diana Rocha, Joana Santos, Jennifer Noro, Tânia D. Tavares, Marta O. Teixeira, Daniela Araújo, Joana Castro, Carina Almeida, Sónia Silva, Helena P. Felgueiras, Artur Ribeiro, Armando Venâncio, Artur Cavaco-Paulo and Carla Silva
Processes 2025, 13(2), 374; https://doi.org/10.3390/pr13020374 - 29 Jan 2025
Viewed by 1195
Abstract
This research aims to explore the potential benefits of natural deep eutectic solvents (NADES) in formulating translucent NADES-in-oil emulsions (TEs) containing essential oils (EOs) for cosmetic applications. The TEs investigated in this study are based on previous formulations, consisting of 50 wt.% egg [...] Read more.
This research aims to explore the potential benefits of natural deep eutectic solvents (NADES) in formulating translucent NADES-in-oil emulsions (TEs) containing essential oils (EOs) for cosmetic applications. The TEs investigated in this study are based on previous formulations, consisting of 50 wt.% egg phosphatidylcholine (EPC), 20 wt.% ethanol, 20 wt.% olive oil (OlO), thyme oil (TO), or oregano oil (OrO), and 10 wt.% NADES made from a 1:1 or 1:4 ratio of lactic acid and glycerol (LA). These emulsions exhibit high antioxidant activity, attributed to the terpenes present in the essential oils, such as thymol in TO and carvacrol in OrO. The TEs containing TO and OrO demonstrated a more fluid consistency, along with a more appealing texture and fragrance compared to the OlO control. Additionally, these emulsions exhibited the ability to permeate pig skin, as well as significant antifungal and antibacterial activity, and low toxicity in the Galleria mellonella larval model. Overall, the findings expand the potential applications of NADES, particularly in the development of translucent emulsions with EO for treating microbial skin and nail infections. Full article
Show Figures

Graphical abstract

19 pages, 862 KiB  
Article
Exploring Alternative Potentialities of Portuguese and Spanish Craft Beers: Antioxidant and Photoprotective Activities
by Maria João Pereira, Diana Santos, Agostinho Cruz, Ângelo Jesus, João P. Martins, Fernando Moreira, Marlene Santos, Cláudia Pinho and Ana Isabel Oliveira
Beverages 2025, 11(1), 11; https://doi.org/10.3390/beverages11010011 - 13 Jan 2025
Viewed by 1624
Abstract
Craft beer has gained popularity due to its unique sensory characteristics and complex matrix with nutritional and potential health benefits. Studies linking beer consumption to skin conditions are limited, however, the high content of bioactive compounds is related to biological activities such as [...] Read more.
Craft beer has gained popularity due to its unique sensory characteristics and complex matrix with nutritional and potential health benefits. Studies linking beer consumption to skin conditions are limited, however, the high content of bioactive compounds is related to biological activities such as antibacterial, anti-inflammatory, anti-oxidative, and anti-carcinogenic. This study aims to evaluate the antioxidant, photoprotective and metabolic activity in human keratinocytes (HaCaT). Eighteen craft and four industrial beers were analyzed after dealcoholizing, degassing and freeze-drying. Total phenolic content (TPC) and antioxidant activity were determined. The most promising craft beer was studied for its photoprotective and metabolic activity. An India Pale Ale beer (ALM-IPA) presented the second best TPC (8.96 ± 0.64 mg of GAE/g) and promising antioxidant activity by ABTS (IC50 = 55.21 ± 4.68 µg/mL), H2O2 (IC50 = 23.54 ± 1.53 µg/mL) and FRAP (53.74 ± 1.27 µmol TE/g) assays. Regarding photoprotective activity, a solar photoprotection factor of 48.85 ± 0.39 was obtained. ALM-IPA showed no cytotoxicity up to a concentration of 250 µg/mL after 24 and 48 h of incubation. The potential benefits of beer extracts on skin can be seen, but further studies are essential to corroborate the findings and guarantee the safety of the extracts. Full article
Show Figures

Figure 1

16 pages, 2438 KiB  
Article
Bioaccumulation Patterns in Different Tissues of Twelve Species of Elasmobranchs from the Tyrrhenian and Ionian Sea (Calabria, Southern Italy)
by Samira Gallo, Francesco Luigi Leonetti, Francesca Romana Reinero, Primo Micarelli, Luigi Passarelli, Gianni Giglio, Concetta Milazzo, Sandra Imbrogno, Donatella Barca, Massimiliano Bottaro and Emilio Sperone
Environments 2025, 12(1), 12; https://doi.org/10.3390/environments12010012 - 3 Jan 2025
Cited by 1 | Viewed by 1061
Abstract
Marine ecosystems are increasingly threatened by pollutants, including trace elements (TEs) such as heavy metals, which bioaccumulate and pose risks to both marine fauna and human health. Sharks and rays are particularly susceptible to metal uptake and retention, making them sentinel species for [...] Read more.
Marine ecosystems are increasingly threatened by pollutants, including trace elements (TEs) such as heavy metals, which bioaccumulate and pose risks to both marine fauna and human health. Sharks and rays are particularly susceptible to metal uptake and retention, making them sentinel species for assessing environmental contamination. This study investigated the bioaccumulation of 16 TEs across 12 elasmobranch species sampled from the Ionian and Tyrrhenian coasts of Calabria, southern Italy, over an 11-year period. Muscle tissue was analyzed for all species, while additional comparisons among skin, muscle, and brain tissues were conducted for Galeus melastomus. Statistical analyses revealed significant variability in TEs concentrations across trophic levels (TRLs), with higher levels observed in species occupying higher trophic positions. Positive correlations were noted for elements such as Al, Ba, and Se, while negative correlations were found for Co, Cu, Mn, and U, indicating species-specific metabolic adaptations. Tissue-specific analyses identified the skin as a primary site for TEs accumulation, likely due to its barrier functions and external exposure. This study highlights the complex interplay of ecological, dietary, and physiological factors influencing TEs bioaccumulation in elasmobranchs and emphasizes the need for further research to understand the implications for marine food webs and conservation strategies. Full article
(This article belongs to the Special Issue Biomonitoring and Risk Assessment of Marine Ecosystems)
Show Figures

Figure 1

15 pages, 1995 KiB  
Article
In Vitro Bioactivity of Australian Finger Lime Cultivars as an Initial Evaluation of Their Nutraceutical Potential
by Joel B. Johnson, Parbat Raj Thani, Si-Yuan Chen, Janice S. Mani, Michael E. Netzel and Mani Naiker
Nutraceuticals 2024, 4(4), 596-610; https://doi.org/10.3390/nutraceuticals4040032 - 24 Oct 2024
Viewed by 2540
Abstract
There is increasing interest in Australian finger lime (Citrus australasica) due to its nutritional and bioactive potential. In this study, polar extracts from five finger lime cultivars were investigated for their potential bioactivity using a range of assays: antioxidant capacity (total [...] Read more.
There is increasing interest in Australian finger lime (Citrus australasica) due to its nutritional and bioactive potential. In this study, polar extracts from five finger lime cultivars were investigated for their potential bioactivity using a range of assays: antioxidant capacity (total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC)), total monomeric anthocyanin content (TMAC), anti-diabetic activity (α-glucosidase and α-amylase inhibition), anti-Alzheimer activity (acetylcholinesterase inhibition), Skin-whitening activity skin-brightening activity (tyrosinase inhibition), and anti-inflammatory activity (COX-2 inhibition). Commercial Tahitian lime was used as a “control” (comparison). The TPC ranged from 328 to 779 mg GAE/100 g dry weight (DW) in the pulp (compared to 1043 mg GAE/100 g for Tahitian lime) and from 755 to 1048 mg GAE/100 g in the peel (1704 mg GAE/100 g for Tahitian lime). A similar range of variation was seen for FRAP, ranging from 114 to 436 mg TE/100 g DW in the pulp (422 mg TE/100 g for Tahitian lime) and 259 to 495 mg TE/100 g DW in the peel (491 mg TE/100 g for Tahitian lime). Similarly, the TFC was generally lower in finger lime pulp (100–392 mg QE/100 g DW) compared to Tahitian lime (312 mg QE/100 g). The polar extracts did not show any significant inhibition of α-glucosidase, α-amylase, tyrosinase, or COX-2. One finger lime variety showed moderate (>50%) inhibition of acetylcholinesterase (AChE) at the highest concentration screened (~1500 mg/L), as did Tahitian lime. Additionally, in silico docking against acetylcholinesterase suggested that some of the polyphenols present, including catechin, quercetin-3-glucoside, and cyanidin-3-glucoside, could potentially dock to AChE and inhibit it. This is the first time the species has been investigated for many of these bioactive properties, and also the first time in silico docking has been performed to explore which potential compounds from this species could provide its bioactivity. Although little bioactivity was generally found across the applied bioassays, these findings nevertheless provide important basic data for future research and any claims about the potential health benefits of Australian finger lime. Full article
Show Figures

Figure 1

20 pages, 27097 KiB  
Article
Quercus infectoria Gall Ethanolic Extract Accelerates Wound Healing through Attenuating Inflammation and Oxidative Injuries in Skin Fibroblasts
by Suttiwan Wunnoo, Decha Sermwittayawong, Rachanida Praparatana, Supayang Piyawan Voravuthikunchai and Chanawee Jakkawanpitak
Antioxidants 2024, 13(9), 1094; https://doi.org/10.3390/antiox13091094 - 9 Sep 2024
Cited by 2 | Viewed by 3200
Abstract
Quercus infectoria Olivier (Fagaceae) nutgall, a traditional Asian medicine, is renowned for its efficacy in treating wounds and skin disorders. Although the gall extract has shown promising results in accelerating wound healing in diabetic animal models, its mechanisms, particularly the effects on redox [...] Read more.
Quercus infectoria Olivier (Fagaceae) nutgall, a traditional Asian medicine, is renowned for its efficacy in treating wounds and skin disorders. Although the gall extract has shown promising results in accelerating wound healing in diabetic animal models, its mechanisms, particularly the effects on redox balance, remain poorly understood. This study aims to investigate the effects and mechanisms of Q. infectoria gall ethanolic extract (QIG) on wound healing in fibroblasts, with a specific emphasis on its modulation of oxidative stress. Hydrogen peroxide (H2O2)-treated L929 cells were used as an in vitro model of oxidation-damaged fibroblasts. QIG exhibited potent antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assay values of 305.43 ± 7.48, 508.94 ± 15.12, and 442.08 ± 9.41 µM Trolox equivalents (TE)/µg, respectively. Elevated H2O2 levels significantly reduced L929 cell viability, with a 50% lethal concentration of 1.03 mM. QIG mitigated H2O2-induced cytotoxicity in a dose-dependent manner, showing protective effects in pre-, post-, and co-treatment scenarios. QIG significantly reduced H2O2-induced intracellular reactive oxygen species production and inflammation-related gene expression (p < 0.05). Additionally, at 25 µg/mL, QIG remarkably improved wound closure in H2O2-treated L929 cells by approximately 9.4 times compared with the H2O2 treatment alone (p < 0.05). These findings suggest QIG has potential therapeutic applications in wound healing, mediated through the regulation of oxidative stress and inflammatory response. Full article
(This article belongs to the Special Issue Antioxidants for Skin Health)
Show Figures

Graphical abstract

19 pages, 4692 KiB  
Article
In Vitro, Ex Vivo, and In Vivo Evaluation of Silver Nanoparticles Synthesized Using Green Tomato Extract: Perspectives on Topical Application
by Daniela Cunha, Catarina Faria-Silva, Filomena A. Carvalho, Lia Ascensão, Pedro Simões, Manuela Carvalheiro and Sandra Simões
Appl. Sci. 2024, 14(16), 7309; https://doi.org/10.3390/app14167309 - 19 Aug 2024
Viewed by 1579
Abstract
Biogenic silver nanoparticles (AgNP) are among the fastest-growing nanomaterials due to the simplicity, efficiency, and sustainability of their biosynthesis using phytochemicals as reducing and coating agents. The agro-food industry generates large quantities of organic waste, a renewable source of biomolecules for AgNP biosynthesis. [...] Read more.
Biogenic silver nanoparticles (AgNP) are among the fastest-growing nanomaterials due to the simplicity, efficiency, and sustainability of their biosynthesis using phytochemicals as reducing and coating agents. The agro-food industry generates large quantities of organic waste, a renewable source of biomolecules for AgNP biosynthesis. The main objective of this work was to prepare and characterize biogenic AgNP using a green tomato waste extract (TE) obtained by subcritical water extraction. To the best of our knowledge, this is the first report on the use of such an extract in the synthesis of AgNP. The effects of the TE and AgNO3 concentrations, reaction time, pH, and temperature on AgNP physico-chemical characteristics and on in vitro cytotoxicity against HaCaT and THP-1 cells were assessed. Antimicrobial activity was determined in vitro and ex vivo. The wound-healing capability of AgNP was evaluated in vivo in an incisional wound mouse model. The developed AgNP have a Surface Plasmon Resonance (SPR) band between 402 and 406 nm and a size of ±60 nm, and they are negatively charged (−42 mV) and spherical. In vitro and ex vivo studies prove that AgNP do not compromise skin cells and can decrease cutaneous irritation. The AgNP formulated in a gel revealed similar wound-healing properties to a commercial silver-containing topical ointment. Overall, the biogenic synthesis of AgNP employing an extract of agricultural waste obtained by an eco-friendly method is simple and cost-effective and presents the potential for application in skin disease management. Full article
(This article belongs to the Special Issue Advances in Bioactive Compounds from Plants and Their Applications)
Show Figures

Figure 1

23 pages, 4207 KiB  
Article
Streamlining Skin Regeneration: A Ready-To-Use Silk Bilayer Wound Dressing
by Anabela Veiga, Inês V. Silva, Juliana R. Dias, Nuno M. Alves, Ana L. Oliveira and Viviana P. Ribeiro
Gels 2024, 10(7), 439; https://doi.org/10.3390/gels10070439 - 30 Jun 2024
Cited by 2 | Viewed by 2382
Abstract
Silk proteins have been highlighted in the past decade for tissue engineering (TE) and skin regeneration due to their biocompatibility, biodegradability, and exceptional mechanical properties. While silk fibroin (SF) has high structural and mechanical stability with high potential as an external protective layer, [...] Read more.
Silk proteins have been highlighted in the past decade for tissue engineering (TE) and skin regeneration due to their biocompatibility, biodegradability, and exceptional mechanical properties. While silk fibroin (SF) has high structural and mechanical stability with high potential as an external protective layer, traditionally discarded sericin (SS) has shown great potential as a natural-based hydrogel, promoting cell–cell interactions, making it an ideal material for direct wound contact. In this context, the present study proposes a new wound dressing approach by developing an SS/SF bilayer construct for full-thickness exudative wounds. The processing methodology implemented included an innovation element and the cryopreservation of the SS intrinsic secondary structure, followed by rehydration to produce a hydrogel layer, which was integrated with a salt-leached SF scaffold to produce a bilayer structure. In addition, a sterilization protocol was developed using supercritical technology (sCO2) to allow an industrial scale-up. The resulting bilayer material presented high porosity (>85%) and interconnectivity while promoting cell adhesion, proliferation, and infiltration of human dermal fibroblasts (HDFs). SS and SF exhibit distinct secondary structures, pore sizes, and swelling properties, opening new possibilities for dual-phased systems that accommodate the different needs of a wound during the healing process. The innovative SS hydrogel layer highlights the transformative potential of the proposed bilayer system for biomedical therapeutics and TE, offering insights into novel wound dressing fabrication. Full article
(This article belongs to the Special Issue Hydrogel for Tissue Engineering and Biomedical Therapeutics)
Show Figures

Figure 1

Back to TopTop