Search Results (58)

Atomic magnetometers based on the spin-exchange relaxation-free (SERF) regime have broad applications in bio-magnetic measurement due to their high sensitivity and miniaturized size. In this paper, we propose a SERF-based magnetometer using 1 × 2 polarization-maintaining fiber (PMF) with single-beam parameter optimization. The impacts of temperature, pumping laser power, and modulation amplitude on the magnetometer’s response signal at the SERF regime are examined. Moreover, through the simulation of zero-field resonance, the compensation accuracy is optimized. To further improve the compensation stability and accuracy, a novel finite state machine (FSM)-assisted iterative optimization magnetic field compensation algorithm is proposed. A pT-level compensation resolution with an error below 1.6% is achieved, which lays the foundation for the subsequent application of biomagnetic measurement arrays. Full article

A stabilization method utilizing MEMS technology combined with a liquid crystal variable retarder (LCVR) was developed to enhance fiber laser output power stability and was applied to a spin-exchange relaxation-free (SERF) atomic magnetometer. Comparative experiments demonstrated that the unstabilized laser output exhibited $2.8\%$ power fluctuations over a 500 s period, while the stabilized laser reduced this to $0.2\%$. Spectral density analysis confirmed suppressed frequency-domain fluctuations, indicating improved robustness against disturbances. Furthermore, the stabilized laser also reduced optical noise in SERF magnetometry, achieving a sensitivity of $19.2\mathrm{fT}/{\mathrm{Hz}}^{1/2}$. These results validate that the method optimizes both time- and frequency-domain performance, thereby advancing high-precision SERF magnetometry. Full article

The beam angle error of the pump light in a K-Rb-²¹Ne spin-exchange relaxation-free atomic co-magnetometer (SERFCM) significantly degrades the efficiency of optical pumping and the system’s ability to suppress magnetic field noise. In this work, a system response model that incorporates the pump beam alignment error (PBAE) is established. The influence of PBAE on the scale factor, bandwidth, and magnetic noise response of the inertial output is analyzed. Theoretical results show that PBAE increases the internal magnetic field gradient, reduces the efficiency of nuclear spin hyperpolarization, and increases the nuclear spin relaxation rate, ultimately degrading the system’s scale factor, bandwidth, and magnetic noise suppression capability. Experimental results demonstrate that, compared to the original SERFCM with PBAE, aligning the pump laser using the proposed method improves the polarization strength of nuclear spins by approximately 10% and enhances magnetic noise suppression by 40%. Full article

Genetic studies and phenotypic expansion of hearing loss (HL) for people living in Africa are greatly needed. We evaluated the clinical phenotypes of three affected siblings presenting non-syndromic (NS) HL and five unaffected members of a consanguineous Ghanaian family. Analysis of exome sequence data was performed for all affected and one unaffected family members. In-depth genetic and cellular characterization studies were performed to investigate biological significance of the implicated variant using bioinformatic tools and cell-based experimentation. Audiological examinations showed severe-to-profound, bilateral, symmetrical, and post-lingual onset. The whole-exome sequencing (WES) identified a homozygous frameshift variant: MARVEL domain containing 2 (MARVELD2):c.1058dup;p.(Val354Serfs*5) in all affected siblings. This frameshift variant leads to an early stop codon insertion and predicted to be targeted by nonsense medicated decay (mutant protein predicted to lack conserved C-terminal domain if translated). Cell immunofluorescence and immunocytochemistry studies exposed the functional impact of the mutant protein’s expression, stability, localization, protein–protein binding, barrier function, and actin cytoskeleton architecture. The identified variant segregates with NSHL in the index Ghanaian family. The data support this nonsense variant as pathogenic, likely to impact the homeostasis of ions, solutes, and other molecules, compromising membrane barrier and signaling in the inner ear spaces. Full article

Bias instability is one of the most critical factors in the performance of spin-exchange relaxation-free (SERF) co-magnetometers. Previous studies on SERF co-magnetometers have shown that changes in the atomic ensemble temperature can lead to variations in the alkali metal atom density, which in turn affect the optical rotation angle and light shift, ultimately influencing the system’s stability. Building on this understanding, this paper introduces the thermoelectric analogy method for the first time in the transient heat transfer analysis of SERF co-magnetometer atomic ensembles. Using this method, the primary factors affecting the atomic ensemble temperature in a SERF co-magnetometer were analyzed, and transient heat transfer models were established for the following processes: the interaction between the non-magnetic electric heating system and the atomic ensemble temperature, laser heating of the atomic ensemble by the optical system, and the effect of environmental temperature changes on the non-magnetic electric heating system. These models were experimentally validated through active temperature variation experiments. The experimental results show that the proposed transient heat transfer models accurately describe the related heat transfer processes of the atomic ensemble, with model fitting accuracy exceeding 98%. This lays a solid foundation for the high-precision closed-loop control of the atomic ensemble temperature in SERF co-magnetometers and provides valuable insights for the structural design and engineering applications of SERF co-magnetometers. Full article

Optically pumped magnetometers (OPMs) functioning in the spin-exchange relaxation-free (SERF) regime have emerged as attractive options for measuring weak magnetic fields, owing to their portability and remarkable sensitivity. The operation of SERF-OPM critically relies on the ambient magnetic field; thus, a magnetic field compensation device is commonly employed to mitigate the ambient magnetic field to near zero. Nonetheless, the bias of the OPM may influence the compensation impact, a subject that remains unexamined. This paper introduced an innovative bias calibration technique for OPMs. The sensitivity of the OPM was altered by adjusting the cell temperature. The output of the OPM was then documented with varying sensitivity. It is assumed that the signal exhibits a linear correlation with the environmental magnetic field, and the statistical characteristics of the magnetic field are identical for both measurements, upon which the bias of the OPM is assessed. The bias was subsequently considered in the feedback magnetic field compensation mechanism. The results indicate that this technique might successfully reduce environmental magnetic fluctuations and enhance the sensitivity of the OPM. This technique measured the magnetic field produced by the human heart, confirming the viability of the ultra-weak biomagnetic field measurement approach. Full article

We propose a non-magnetic transparent heating film based on silver nanowires (Ag-NWs) for application in spin-exchange relaxation-free (SERF) magnetic field measurement devices. To achieve ultra-high sensitivity in atomic magnetometers, the atoms within the alkali metal vapor cell must be maintained in a stable and uniform high-temperature environment. Ag-NWs, as a transparent conductive material with exceptional electrical conductivity, are well suited for this application. By employing high-frequency AC heating, we effectively minimize associated magnetic noise. The experimental results demonstrate that the proposed heating film, utilizing a surface heating method, can achieve temperatures exceeding 140 °C, which is sufficient to vaporize alkali metal atoms. The average magnetic flux coefficient of the heating film is 0.1143 nT/mA. Typically, as the current increases, a larger magnetic field is generated. When integrated with the heating system discussed in this paper, this characteristic can effectively mitigate low-frequency magnetic interference. In comparison with traditional flexible printed circuits (FPC), the Ag-NWs heating film exhibits a more uniform temperature distribution. This magnetically transparent heating film, leveraging Ag-NWs, enhances atomic magnetometry and presents opportunities for use in chip-level gyroscopes, atomic clocks, and various other atomic devices. Full article

Familial dysbetalipoproteinemia (FD) is a highly atherogenic, prevalent genetically based lipid disorder. About 10% of FD patients have rare APOE variants associated with autosomal dominant FD. However, there are insufficient data on the relationship between rare APOE variants and FD. Genetic data from 4720 subjects were used to identify rare APOE variants and investigate their pathogenicity for autosomal dominant FD. We observed 24 variants in 86 unrelated probands. Most variants were unique (66.7%). Five identified APOE variants (p.Glu63ArgfsTer15, p.Gly145AlafsTer97, p.Lys164SerfsTer87, p.Arg154Cys, and p.Glu230Lys) are causal for autosomal dominant FD. One of them (p.Lys164SerfsTer87) was described for the first time. When we compared clinical data, it was found that carriers of pathogenic or likely pathogenic APOE variants had significantly higher triglyceride levels (median 5.01 mmol/L) than carriers of benign or likely benign variants (median 1.70 mmol/L, p = 0.034) and variants of uncertain significance (median 1.38 mmol/L, p = 0.036). For the first time, we estimated the expected prevalence of causal variants for autosomal dominant FD in the population sample: 0.27% (one in 619). Investigating the spectrum of APOE variants may advance our understanding of the genetic basis of FD and underscore the importance of APOE gene sequencing in patients with lipid metabolism disorders. Full article

Background: Recently identified Hero proteins, which possess chaperone-like functions, are promising candidates for research into atherosclerosis-related diseases, including ischemic stroke (IS). Methods: 2204 Russian subjects (917 IS patients and 1287 controls) were genotyped for fifteen common SNPs in Hero20 gene C11orf58 using probe-based PCR and the MassArray-4 system. Results: Six C11orf58 SNPs were significantly associated with an increased risk of IS in the overall group (OG) and significantly modified by smoking (SMK) and low fruit/vegetable intake (LFVI): rs10766342 (effect allele (EA) A; P(_OG = 0.02; _SMK = 0.009; _LFVI = 0.04)), rs11024032 (EA T; P(_OG = 0.01; _SMK = 0.01; _LFVI = 0.036)), rs11826990 (EA G; P(_OG = 0.007; _SMK = 0.004; _LFVI = 0.03)), rs3203295 (EA C; P(_OG = 0.016; _SMK = 0.01; _LFVI = 0.04)), rs10832676 (EA G; P(_OG = 0.006; _SMK = 0.002; _LFVI = 0.01)), rs4757429 (EA T; P(_OG = 0.02; _SMK = 0.04; _LFVI = 0.04)). The top ten intergenic interactions of Hero genes (two-, three-, and four-locus models) involved exclusively polymorphic loci of C11orf58 and C19orf53 and were characterized by synergic and additive (independent) effects between SNPs. Conclusions: Thus, C11orf58 gene polymorphism represents a major risk factor for IS. Bioinformatic analysis showed the involvement of C11orf58 SNPs in molecular mechanisms of IS mediated by their role in the regulation of redox homeostasis, inflammation, vascular remodeling, apoptosis, vasculogenesis, neurogenesis, lipid metabolism, proteostasis, hypoxia, cell signaling, and stress response. In terms of intergenic interactions, C11orf58 interacts most closely with C19orf53. Full article

This paper systematically studies the output noise model of the K-Rb-²¹Ne co-magnetometer and proposes the method for determining the optimal pump laser power density. The amplitude-frequency response and the equivalent model for each frequency band are obtained through the transfer function of the co-magnetometer. Based on the established model and considering the power spectral density characteristics of magnetic noise, the output noise equation is formulated. Consequently, the pump laser power density yielding minimal output noise is determined. Both experimental and simulation results indicate that the pump laser power density yielding minimal output noise is greater than the pump laser power density corresponding to the maximum scale factor. Moreover, when the co-magnetometer operates at the pump laser power density corresponding to the minimal output noise, the output noise can be reduced by approximately 25%, and the Allan variance reaches its optimal value. The optimal Allan variance at 180 °C and 190 °C are 0.01395°/h @100 s and 0.01329°/h @100 s, respectively. Therefore, this pump laser power density is designated as the optimal pump laser power density for the co-magnetometer. Finally, simulations are conducted to investigate the variation patterns of the optimal pump laser power density points and the minimum output noise under different density ratios and gas pressures. The theories and methods proposed in this paper provide significant reference value for selecting the optimal pump laser power density and suppressing magnetic noise in co-magnetometers. Full article

Thyroid dyshormonogenesis (THD) is a heterogeneous group of genetic diseases caused by the total or partial defect in the synthesis or secretion of thyroid hormones. Genetic variants in DUOX2 can cause partial to total iodination organification defects and clinical heterogeneity, from transient to permanent congenital hypothyroidism. The aim of this study was to undertake a molecular characterization and genotype–phenotype correlation in patients with THD and candidate variants in DUOX2. A total of 31 (19.38%) patients from the Catalan Neonatal Screening Program presented with variants in DUOX2 that could explain their phenotype. Fifteen (48.39%) patients were compound heterozygous, 10 (32.26%) heterozygous, and 4 (12.90%) homozygous. In addition, 8 (26.67%) of these patients presented variants in other genes. A total of 35 variants were described, 10 (28.57%) of these variants have not been previously reported in literature. The most frequent variant in our cohort was c.2895_2898del/p.(Phe966SerfsTer29), classified as pathogenic according to reported functional studies. The final diagnosis of this cohort was permanent THD in 21 patients and transient THD in 10, according to reevaluation and/or need for treatment with levothyroxine. A clear genotype–phenotype correlation could not be identified; therefore, functional studies are necessary to confirm the pathogenicity of the variants. Full article

Developmental and epileptic encephalopathy-9 (DEE9) is characterized by seizure onset in infancy, mild to severe intellectual impairment, and psychiatric features and is caused by a mutation in the PCDH19 gene on chromosome Xq22. The rare, unusual X-linked type of disorder affects heterozygous females and mosaic males; transmitting males are unaffected. In our study, 165 patients with epilepsy were tested by Next Generation Sequencing (NGS)-based panel and exome sequencing using Illumina technology. PCDH19 screening identified three point mutations, one indel, and one 29 bp-long deletion in five unrelated female probands. Two novel mutations, c.1152_1180del (p.Gln385Serfs*6) and c.830_831delinsAA (p.Phe277*), were identified and found to be de novo pathogenic. Moreover, among the three inherited mutations, two originated from asymptomatic mothers and one from an affected father. The PCDH19 c.1682C>T and c.1711G>T mutations were present in the DNA samples of asymptomatic mothers. After targeted parental testing, X chromosome inactivation tests and Sanger sequencing were carried out for mosaicism examination on maternal saliva samples in the two asymptomatic PCDH19 mutation carrier subjects. Tissue mosaicism and X-inactivation tests were negative. Our results support the opportunity for reduced penetrance in DEE9 and contribute to expanding the genotype–phenotype spectrum of PCDH19-related epilepsy. Full article

The K-Rb-²¹Ne co-magnetometer exhibits poorer dynamic performance due to the larger equivalent magnetic field generated by alkali metal atoms. In this study, the impact of the atomic number density of alkali metal atoms and noble gas atoms in the cell on the dynamic performance of the atomic ensemble is investigated quantitatively. Relationships between the slow-decay term in the transient response attenuation of the Spin-Exchange Relaxation-Free (SERF) co-magnetometer to interference magnetic fields and the number densities of noble gas atoms as well as alkali metal atoms are established. Based on the established model, the relationship between the number density of ²¹Ne atoms and dynamic performance is investigated using cells with five different noble gas pressures. Then, we investigate the impact of the number density of alkali metal atoms using a cell with a pressure of 2.1 atm at different temperatures. The results indicate that, as the number density of alkali metal atoms or noble gas atoms in the cell increases, the dynamic performance of the system improves, which provides a theoretical basis for the design of cell parameters for SERF co-magnetometers. Full article

The risk of oil spills in the Arctic is growing rapidly as anthropogenic activities increase due to climate-driven sea ice loss. Detecting and monitoring fuel spills in the marine environment is imperative for enacting an efficient response to mitigate the risk. Microwave radar systems can be used to address this issue; therefore, we examined the potential of C-band polarimetric radar for detecting diesel fuel in freezing seawater under windy environmental conditions. We present results from a mesocosm experiment, where we introduced diesel fuel to a seawater-filled cylindrical tub at the Sea-ice Environmental Research Facility (SERF), University of Manitoba. We characterized the temporal evolution of the diesel-contaminated seawater and sea ice by monitoring the normalized radar cross section (NRCS) and polarimetric parameters (i.e., copolarization ratio (R_co), cross-polarization ratio (R_xo), entropy (H), mean-alpha (α), conformity coefficient (μ), and copolarization correlation coefficient (ρ_co)) at 20° and 25° incidence angles. Three stages were identified, with notably different NRCS and polarimetric results, related to the thermophysical conditions. The transition from calm conditions to windy conditions was detected by the 25° incidence angle, whereas the transition from open water to sea ice was more apparent at 20°. The polarimetric analysis demonstrated that the conformity coefficient can have distinctive sensitivities to the presence of wind and sea ice at different incidence angles. The H versus α scatterplot showed that the range of distribution is dependent upon wind speed, incidence angle, and oil product. The findings of this study can be used to further improve the capability of existing and future C-band dual-polarization radar satellites or drone systems to detect and monitor potential diesel spills in the Arctic, particularly during the freeze-up season. Full article

Generalized arterial calcification of infancy (GACI) is a rare autosomal-recessive disease characterized by extensive arterial calcification in infancy, with clinical manifestations such as arterial stenoses and heart failure. The ENPP1 inactivation mutation has been identified as a potential defect in most of the cases of GACI, while mutations in ABCC6 are demonstrated in patients who are genotyped as pseudoxanthoma elasticum and only limited cases of GACI are reported. Whole-exome sequencing was applied for the detection of pathogenic variants. Copy-number variants of pathogenic genes were also evaluated through a bioinformatic process and were further validated by real-time quantitative PCR. In this report, we described the clinical information and treatment of a patient with extensive arterial calcification. We have identified the underlying cause as biallelic mutations in ABCC6 (NM_00117: exon30, c.4223_4227dupAGCTC p.(Leu1410Serfs*56)) and a unique exonic deletion that spans from the first to the fourth exons of ABCC6 (chr16:16313388-16330869)). This discovery was made by utilizing a combined genetic testing approach. With the review of previously reported GACI patients with ABCC6 mutation, our work contributed to enriching the mutation spectrum of GACI and providing further information on this rare form of inherited disorder. Full article