Search Results (246)

The increase in the number of wildfires in recent years in different parts of the world has caused growing concern among the population, since the consequences of these fires go beyond the destruction of the ecosystem. With the growing relevance of the Internet of Things (IoT) industry, developing solutions for the early detection of fires is of critical importance. This paper proposes a low-cost network based on Long-Range (LoRa) technology to autonomously assess the level of fire risk and the presence of a fire in rural areas. The system consists of several LoRa nodes with sensors to measure environmental variables such as temperature, humidity, carbon monoxide, air quality, and wind speed. The data collected is sent to a central gateway, where it is stored, processed, and later sent to a website for graphical visualization of the results. In this paper, a survey of the requirements of the devices and sensors that compose the system was made. After this survey, a market study of the available sensors was carried out, ending with a comparison between the sensors to determine which ones met the objectives. Using the chosen sensors, a study was made of possible power solutions for this prototype, considering the expected conditions of use. The system was tested in a real environment, and the results demonstrate that it is possible to cover a circular area with a radius of 2 km using a single gateway. Our system is prepared to trigger fire hazard alarms when, for example, the signals for relative humidity, ambient temperature, and wind speed are below or equal to 30%, above or equal to 30 °C, and above or equal to 30 m/s, respectively (commonly known as the 30-30-30 rule). Full article

Proto-oncogenes in the RAS superfamily play dual roles in maintaining cellular homeostasis, such as regulating growth signals and contributing to cancer development through proliferation and deregulation. Activating proto-oncogenes in vitro transforms cells, underscoring their centrality in gene regulation and cellular networks. Despite decades of research, poor outcomes in advanced cancers reveal gaps in understanding Ras-driven mechanisms or therapeutic strategies. This narrative review examines RAS genes and Ras proteins in both housekeeping functions, such as cell growth, apoptosis, and protein trafficking, as well as in tumorigenesis, integrating insights from human (HRAS, KRAS, NRAS), mouse (Hras, Kras, Nras), and Drosophila melanogaster (ras) models. While RAS mutations are tightly linked to human tumors, the interplay between their standard and oncogenic functions remains complex. Even within the same tissue, distinct cancer pathways—such as the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways—can drive varied disease courses, complicating treatment. Advanced-stage cancers add further challenges, including heterogeneity, protective microenvironments, drug resistance, and adaptive progression. This synthesis organizes current knowledge of RAS gene regulation and Ras protein function from genomic alterations and intracellular signaling to membrane dynamics and extracellular interactions, offering a layered perspective on the Ras pathway’s role in both housekeeping and tumorigenic contexts. Full article

The proliferation of Internet of Things (IoT) swarms—comprising billions of low-end interconnected embedded devices—has transformed industrial automation, smart homes, and agriculture. However, these swarms are highly susceptible to firmware anomalies that can propagate across nodes, posing serious security threats. To address this, we propose a novel Remote Attestation (RA) framework for real-time firmware verification, leveraging Relational Graph Neural Networks (RGNNs) to model the graph-like structure of IoT swarms and capture complex inter-node dependencies. Unlike conventional Graph Neural Networks (GNNs), RGNNs incorporate edge types (e.g., Prompt, Sensor Data, Processed Signal), enabling finer-grained detection of propagation dynamics. The proposed method uses runtime Static Random Access Memory (SRAM) data to detect malicious firmware and its effects without requiring access to firmware binaries. Experimental results demonstrate that the framework achieves 99.94% accuracy and a 99.85% anomaly detection rate in a 4-node swarm (Swarm-1), and 100.00% accuracy with complete anomaly detection in a 6-node swarm (Swarm-2). Moreover, the method proves resilient against noise, dropped responses, and trace replay attacks, offering a robust and scalable solution for securing IoT swarms. Full article

Rhabdomyosarcoma (RMS), the most common pediatric soft tissue sarcoma, comprises embryonal (ERMS) and alveolar (ARMS) subtypes with distinct histopathological features, clinical outcomes, and therapeutic responses. To better characterize their molecular distinctions, we performed untargeted plasma proteomics and metabolomics profiling in children with ERMS (n = 18), ARMS (n = 17), and matched healthy controls (n = 18). Differential expression, functional enrichment (GO, KEGG, RaMP-DB), co-expression network analysis (WGCNA/WMCNA), and multi-omics integration (DIABLO, MOFA) revealed distinct molecular signatures for each subtype. ARMS displayed elevated oncogenic and stemness-associated proteins (e.g., cyclin E1, FAP, myotrophin) and metabolites involved in lipid transport, fatty acid metabolism, and polyamine biosynthesis. In contrast, ERMS was enriched in immune-related and myogenic proteins (e.g., myosin-9, SAA2, S100A11) and metabolites linked to glutamate/glycine metabolism and redox homeostasis. Pathway analyses highlighted subtype-specific activation of PI3K-Akt and Hippo signaling in ARMS and immune and coagulation pathways in ERMS. Additionally, the proteomics and metabolomics datasets showed association with clinical parameters, including disease stage, lymph node involvement, and age, demonstrating clear molecular discrimination consistent with clinical observation. Co-expression networks and integrative analyses further reinforced these distinctions, uncovering coordinated protein–metabolite modules. Our findings reveal novel, subtype-specific molecular programs in RMS and propose candidate biomarkers and pathways that may guide precision diagnostics and therapeutic targeting in pediatric sarcomas. Full article

Background: Dendritic cells (DCs) play an important role as a bridge between innate and adaptive immunity, and changes in gene expression of DCs during the immune response to Mycobacterium tuberculosis (M.tb) may affect the development of tuberculosis. Methods: Using systems biology methods, mRNA and miRNA expression profile data of DCs infected with M.tb were obtained. A total of 1398 differentially expressed mRNAs and 79 differentially expressed miRNAs were identified, and a corresponding miRNA–mRNA regulatory network was constructed using Cytoscape 3.9.1 software. The functional annotations and pathway classifications of the miRNA–mRNA network were identified using the DAVID tool. Then, the key pathway modules in the miRNA–mRNA network were screened and subjected to PPI network analysis to identify hub nodes. Subsequently the miRNA/mRNA axis was determined, validated by qRT-PCR, and evaluated through ROC curve analysis. Results: The TNF signaling pathway and the Tuberculosis pathway were key pathway modules, with miR-34a-3p/TNF and miR-190a-3p/IL1B being the greatest correlations with the two pathway modules. qRT-PCR results showed that IL1B and miR-190a-3p exhibited significant differences in both the H37Ra and BCG infection groups. The AUC of two factors (IL1B and miR-190a-3p) was 0.9561 and 0.9625, respectively, showing high sensitivity and specificity. Conclusions: Consequently, miR-190a-3p/IL1B might be a good candidate marker to characterize the immune response of DCs to M.tb and a transition signal from innate to adaptive immunity. Full article

The advancement of Internet of Things (IoT) technology has enabled battery-powered devices to be deployed across a wide range of applications; however, it also introduces challenges such as high energy consumption and security vulnerabilities. To address these issues, adiabatic logic circuits offer a promising solution for achieving energy efficiency and enhancing the security of IoT devices. Adiabatic logic circuits are well suited for energy harvesting systems, especially in applications such as sensor nodes, RFID tags, and other IoT implementations. In these systems, the harvested bipolar sinusoidal RF power is directly used as the power supply for the adiabatic logic circuit. However, adiabatic circuits require a peak detector to provide bulk biasing for pMOS transistors. To meet this requirement, a diode-connected MOS transistor-based voltage doubler circuit is used to convert the sinusoidal input into a usable DC signal. In this paper, we propose a novel adiabatic logic design that maintains low power consumption while optimizing energy and current fluctuations across various input transitions. By ensuring uniform and complementary current flow in each transition within the logic circuit’s functional blocks, the design reduces energy variation and enhances resistance against power analysis attacks. Evaluation under different clock frequencies and load capacitances demonstrates that the proposed adiabatic logic circuit exhibits lower fluctuation and improved security, particularly at load capacitances of 50 fF and 100 fF. The results show that the proposed circuit achieves lower power dissipation compared to conventional designs. As an application example, we implemented an ultrasonic transmitter circuit within a LoRaWAN network at the end-node sensor level, which serves as both a communication protocol and system architecture for long-range communication systems. Full article

Malignant melanoma (MM) affects not only humans but also animals, with gray horses being particularly predisposed to acquiring the disease. Multiomics have greatly advanced the understanding of human MM. In contrasty little is known regarding the pathogenesis of gray-horse melanoma and the unique phenomenon of melanoma “dormancy” in some animals. To help close this gap in knowledge, melanoma tissue and intact skin collected from gray horses were subjected to transcriptome analysis using RNAseq. In the next step, cultured primary tumor cells and normal skin fibroblasts were established from gray horses, and their protein expression profiles were determined. The obtained data unambiguously identified gray-horse melanoma (ghM) as a malignant tumor, as reflected by the overrepresentation of pathways typically activated in human melanoma and other human cancers. These included the RAS/RAF/MAPK, the IRS/IGF1R, and the PI3K/AKT signaling networks. In addition, the obtained data suggest that the key molecules RAC1, RAS, and BRAF, which are frequently mutated in human melanoma, may also contain activating mutations in ghM, whilst PTEN may harbor loss-of-function mutations. This issue will be subject to downstream analyses determining the mutational status in ghM to further advance the understanding of this frequent disease in gray horses. Full article

Background/Objectives: Rheumatoid arthritis (RA) is a chronic, systemic, and progressive autoimmune–inflammatory disease primarily affecting small joints. Inonotus obliquus polysaccharide (IOP) is the main component of the parasitic fungus obliquus, which has anti-tumor, anti-inflammatory, and antioxidant effects. However, whether IOP has a therapeutic effect on RA is still unclear. Thus, this study aimed to reveal the effect of IOP on MH7A cells and collagen-induced arthritis (CIA) rats and to investigate the molecular mechanism of IOP in RA. Methods: In this study, network pharmacology was used to identify the key signaling pathways in IOP treatment of RA. The effect of IOP was verified in rats with CIA. We performed CCK-8, EdU, colony formation assay, cell apoptosis, cell migration and invasion, Western blot analysis, and immunofluorescence to elucidate the effect of IOP on the proliferation, apoptosis, migration and invasion of MH7A cells and revealed its modulation of the NF-κB and NLRP3 inflammasome signaling pathways. Results: IOP treatment of CIA rats significantly alleviated joint swelling, synovial tissue proliferation and erosion, and reduced the expression of inflammatory factors TNF-α, IL-6, IL-1β and IL-18. In vitro, IOP significantly inhibited the proliferation, migration, and invasion abilities of TNF-α-stimulated MH7A cells and promoted their apoptosis. Mechanistically, IOP inhibited the NF-κB and NLRP3 inflammasome activation. Conclusions: This study revealed that IOP exerts anti-RA effects by downregulating the NF-κB and NLRP3 inflammasome signaling pathways, promoting cell apoptosis, and inhibiting the expression of inflammatory cytokines, representing a promising therapeutic option for RA. Full article

Background: Rheumatoid arthritis (RA) is a chronic autoimmune disorder that predominantly affects synovial joints, leading to inflammation, pain, and progressive joint damage. Despite therapeutic advancements, the molecular basis of established RA remains poorly defined. Methods: In this study, we conducted an untargeted plasma proteomic analysis using two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in samples from RA patients and healthy controls in the discovery phase. Results: Significantly (ANOVA, p ≤ 0.05, fold change > 1.5) differentially abundant proteins (DAPs) were identified. Notably, upregulated proteins included mitochondrial dicarboxylate carrier, hemopexin, and 28S ribosomal protein S18c, while CCDC124, osteocalcin, apolipoproteins A-I and A-IV, and haptoglobin were downregulated. Receiver operating characteristic (ROC) analysis identified CCDC124, osteocalcin, and metallothionein-2 with high diagnostic potential (AUC = 0.98). Proteins with the highest selected frequency were quantitatively verified by multiple reaction monitoring (MRM) analysis in the validation cohort. Bioinformatic analysis using Ingenuity Pathway Analysis (IPA) revealed the underlying molecular pathways and key interaction networks involved STAT1, TNF, and CD40. These central nodes were associated with immune regulation, cell-to-cell signaling, and hematological system development. Conclusions: Our combined proteomic and bioinformatic approaches underscore the involvement of dysregulated immune pathways in RA pathogenesis and highlight potential diagnostic biomarkers. The utility of these markers needs to be evaluated in further studies and in a larger cohort of patients. Full article

Water pollution from pesticides is a major concern for regulatory agencies worldwide due to expensive detecting mechanisms, delays in the processing of results, and the complexity of the chemical analysis. However, the deployment of monitoring systems utilising the internet of things (IoT) and machine-to-machine communication technologies (M2M) holds promise in overcoming this major global challenge. In this current research, an IoT-based wireless sensor network (WSN) is successfully deployed in rural Kenya at the Kiu watershed, providing in situ pesticide detections and a real-time data visualisation of shallow wells. Kiu is an off-grid community located in an area of intensive agriculture, where residents face a high exposure to pesticides due to farming activities and a reliance on shallow wells for domestic water. The evaluation of path loss models utilising channel characteristics obtained from this study indicate a marked departure from the continuous signal decay with distance. Transmitted packets from deployed sensor nodes indicate minimal mutations of payloads, underscoring systems reliability and data transmission integrity. Additionally, the proposed design significantly reduces the time taken to deliver pesticide measurement results to relevant stakeholders. For the entire monitoring period, pesticide residues were not detected in the selected wells, an outcome validated with lab procedures. These results are attributed to prevailing dry weather conditions which limited the leaching of pesticides to lower layers reaching the water table. Full article

Long Range (LoRa) is one of the promising Low-Power Wide-Area Network technologies to achieve a strong anti-noise ability due to the modulation of the chirp spread spectrum in low-power and long-distance communications. However, LoRa suffers the problem of packet collisions. Hence, we propose QR−LoRa, a novel PHY-layer scheme that can transmit data in both amplitude and frequency dimensions simultaneously. For the amplitude modulation, we modulate the constant envelope of a LoRa chirp with a cyclic right-shifted ramp signal, where the cyclic right-shifted position carries the data of the amplitude modulation. We adopt the standard LoRa for frequency modulation. We prototype QR−LoRa on the software-defined radio platform USRP N210 and evaluate its performance via simulations and field experiments. The results show the bit rate gain of QR−LoRa is up to 2× compared with the standard LoRa device. Full article

The mechanistic target of the Rapamycin complex 1 (mTORC1) signaling pathway plays a pivotal role in regulating crucial life processes, including cell growth and proliferation, by sensing and integrating various signals, such as growth factors, energy status, and amino acids. Our previous studies showed that activation of the mTORC1 signaling pathway enhances silk protein synthesis and silk gland size. Here, the potential of the molecular mechanism mTORC1 to regulate the growth and development of silk gland cells was investigated. Inhibiting mTORC1 with rapamycin decreased proliferation in the Bombyx mori embryonic (BmE) cells and endoreplication in silk gland cells, reducing CyclinB and CyclinE protein levels and DNA content, and arresting the BmE cell cycle at G2/M. Conversely, the overexpression of Ras homolog enriched in brain (Rheb) led to increased proliferation of BmE cells and endoreplication in silk gland cells, as well as a significant elevation in DNA content. This study provides a molecular explanation for the increase in silk protein synthesis and silk gland length through the activation of mTORC1, thereby refining the regulatory network of the silkworm endoreplication and providing new molecular targets for breeding high-yield varieties of Bombyx mori. Full article

With rapid advancements in the Internet of Things (IoT), Low-Power Wide-Area Networks (LPWANs) play a crucial role in expanding IoT’s capabilities while using minimal energy. Among the various LPWAN technologies, LoRaWAN (Long-Range Wide-Area Network) is particularly notable for its capacity to enable long-range, low-rate communications with low power needs. This study investigates how to optimize the placement of LoRaWAN gateways by using a combination of Particle Swarm Optimization (PSO) and Differential Evolution (DE). The approach is validated through simulations driven by HTZ to evaluate network performance in urban settings. Centered around the area of the Politehnica University of Bucharest, this research examines how different gateway placements on various floors of a building affect network coverage and packet loss. The experiment employs Adeunis Field Test Devices (FTDs) and Dragino LG308-EC25 gateways, systematically testing two spreading factors, SF7 and SF12, to assess their effectiveness in terms of signal quality and reliability. An innovative optimization algorithm, GateOpt PSODE, is introduced, which combines PSO and DE to optimize gateway placements based on real-time network performance metrics, like the Received Signal Strength Indicator (RSSI), the Signal-to-Noise Ratio (SNR), and packet loss. The findings reveal that strategically positioning gateways, especially on higher floors, significantly improves communication reliability and network efficiency, providing a solid framework for deploying LoRaWAN networks in intricate urban environments. Full article

In recent years, with the rapid development of the Internet and mobile devices, the high-resolution video industry has ushered in a booming golden era, making video content the primary driver of Internet traffic. This trend has spurred continuous innovation in efficient video coding technologies, such as Advanced Video Coding/H.264 (AVC), High Efficiency Video Coding/H.265 (HEVC), and Versatile Video Coding/H.266 (VVC), which significantly improves compression efficiency while maintaining high video quality. However, during the encoding process, compression artifacts and the loss of visual details remain unavoidable challenges, particularly in high-resolution video processing, where the massive amount of image data tends to introduce more artifacts and noise, ultimately affecting the user’s viewing experience. Therefore, effectively reducing artifacts, removing noise, and minimizing detail loss have become critical issues in enhancing video quality. To address these challenges, this paper proposes a post-processing method based on Convolutional Neural Network (CNN) that improves the quality of VVC-reconstructed frames through deep feature extraction and fusion. The proposed method is built upon a high-resolution dual-path residual gating system, which integrates deep features from different convolutional layers and introduces convolutional blocks equipped with gating mechanisms. By ingeniously combining gating operations with residual connections, the proposed approach ensures smooth gradient flow while enhancing feature selection capabilities. It selectively preserves critical information while effectively removing artifacts. Furthermore, the introduction of residual connections reinforces the retention of original details, achieving high-quality image restoration. Under the same bitrate conditions, the proposed method significantly improves the Peak Signal-to-Noise Ratio (PSNR) value, thereby optimizing video coding quality and providing users with a clearer and more detailed visual experience. Extensive experimental results demonstrate that the proposed method achieves outstanding performance across Random Access (RA), Low Delay B-frame (LDB), and All Intra (AI) configurations, achieving BD-Rate improvements of 6.1%, 7.36%, and 7.1% for the luma component, respectively, due to the remarkable PSNR enhancement. Full article

Effective analysis of central bank communications is critical for anticipating monetary policy changes and guiding market expectations. However, traditional natural language processing models face significant challenges in processing lengthy and nuanced policy documents, which often exceed tens of thousands of tokens. This study addresses these challenges by proposing a novel integrated deep learning framework based on Hyena Hierarchy architectures, which utilize sub-quadratic convolution mechanisms to efficiently process ultra-long sequences. The framework employs Delta-LoRA (low-rank adaptation) for parameter-efficient fine-tuning, updating less than 1% of the total parameters without additional inference overhead. To ensure robust performance across institutions and policy cycles, domain-adversarial neural networks are incorporated to learn domain-invariant representations, and a multi-task learning approach integrates auxiliary hawkish/dovish sentiment signals. Evaluations conducted on a comprehensive dataset comprising Federal Open Market Committee statements and European Central Bank speeches from 1977 to 2024 demonstrate state-of-the-art performance, achieving over 6% improvement in macro-F1 score compared to baseline models while significantly reducing inference latency by 65%. This work offers a powerful and efficient new paradigm for handling ultra-long financial policy texts and demonstrates the effectiveness of integrating advanced sequence modeling, efficient fine-tuning, and domain adaptation techniques for extracting timely economic signals, with the aim to open new avenues for quantitative policy analysis and financial market forecasting. Full article