Search Results (904)

Background: The real-world risk profiles of newly defined steatotic liver disease (SLD) subtypes—MASLD, MetALD, and ALD—remain incompletely described in community settings. Methods: A cross-sectional analysis of 950 health-checkup participants was conducted. SLD (CAP ≥ 248 dB/m) and significant fibrosis (LSM ≥ 7.0 kPa) were evaluated by transient elastography. Associations between alcohol intake, cardiometabolic factors, fibrosis, and gut microbiota (16S rRNA sequencing) were assessed. Results: Among 950 participants, 310 (33%) had SLD (MASLD, n = 222; MetALD, n = 41; ALD, n = 23). Treated as a continuous exposure, higher alcohol intake was significantly correlated with elevated systolic/diastolic blood pressure, triglycerides, AST, and γ-GTP, but inversely correlated with HOMA-IR (all p < 0.05). In multivariable logistic regression adjusting for cardiometabolic factors, BMI was the only independent predictor of fibrosis (adjusted OR 1.22, 95% CI 1.11–1.35, p < 0.01), whereas alcohol intake showed no independent association. Furthermore, microbiota analysis revealed that ALD-related SLD was characterized by significant depletion of Blautia and enrichment of Gemella (FDR q < 0.05) compared to non-SLD controls, indicating an alcohol-associated dysbiosis signature. Conclusions: In early-stage SLD, alcohol intake continuously exacerbates cardiometabolic risk factors, whereas fibrosis is predominantly driven by BMI. These findings support quantitative alcohol/BMI integration for risk stratification, alongside microbiota profiling to detect ALD-related dysbiosis. Full article

Managers of protected areas (PAs) face growing challenges to conserve biodiversity while responding to multiple land uses such as recreation, tourism, and resource extraction. These pressures are intensified by the impacts of climate change on ecosystems. This highlights the need for planning approaches that support decision-making in the short, medium, and long term. This article profiles Wells Gray Provincial Park as a case study to demonstrate how an ecosystem-based planning approach can be incorporated into PAs planning. Wells Gray is situated in a unique ecosystem in the interior of British Columbia (Canada). We present an innovative model that integrates land cover types, ecosystem mapping, and Biogeoclimatic (BGC) zones derived from the Biogeoclimatic Ecosystem Classification (BEC) system using GIS tools to identify ecosystems and their associated services as Critical Decision Factors (CDFs). By explicitly linking ecosystems, land cover, and spatial patterns, this approach supports the systemic inclusion of ecosystems in management decisions. To account for future uncertainty, BGC zones were projected under climate change scenarios to inform interpretations of potential ecosystem impacts. The results indicate that this integrated analysis can initiate strategic thinking and facilitate dialogue to collaboratively plan with stakeholders. This approach can improve ecosystem-based planning processes in PAs across Canada. Full article

The use of confuser–diffuser nozzles in power machines enables efficient conversion of gas energy into mechanical work. However, traditional Laval, Venturi, and Vitoszynski nozzles are associated with shock wave formation, causing energy losses, noise, and structural loading. This study proposes innovative jet nozzles with an internal streamlined body that forms annular flow rather than a classical diffusor. A rational computational design methodology based on the Venturi effect criterion and equality of cross-sectional area variation laws was developed. A couple of configurations with spindle-toroidal and ellipsoidal streamlined bodies were generated analytically, studied numerically, and confirmed experimentally. Based on the SST turbulence model, CFD simulations for a compressible flow (air) show that the proposed designs reduce the pressure jump from 60 kPa (traditional nozzle) to 20 kPa for the spindle-toroidal configuration and eliminate it for the ellipsoidal configuration. The Reynolds number in the throat decreases by a factor of 2.6, reducing turbulence. The outlet velocity increases by 3.0% for the spindle-toroidal design, while the ellipsoidal nozzle provides expansion with slightly lower velocity but a smoother velocity profile. Experimental thrust measurements agree with simulations within 2.6–6.7%. The proposed designs enhance energy efficiency, reduce erosion and vibration, and enable adaptive flow control via axial displacement of the streamlined body. Full article

The use of computational fluid dynamics (CFD) to study hemodynamics in arteries offers significant potential for addressing complex flow problems. Due to its enhanced performance hardware and software, CFD has become an important approach for studying hemodynamics in human arteries. This approach is utilized to investigate hemodynamics and forecast risk factors for atherosclerotic lesion development and progression, including circulatory flow, and to analyze local flow fields and flow profiles resulting from geometric changes. This foundational study will aid in analyzing blood flow behavior through the abdominal aorta and the origin and courses of renal arteries, as well as investigating the causes of disorders such as atherosclerosis and hypertension. The current study investigates three idealized abdominal aorta–renal artery junction models under varying blood pressure settings. Materialise software V19 was used to extract the geometry data to create idealized 3D abdominal aorta–renal branching models. Unsteady flow simulations were performed in ANSYS Fluent, utilizing rigid walls and Newtonian and Carreau–Yasuda viscosity conditions. Oscillatory shear index (OSI) and Time-averaged wall shear stress (TAWSS) were measured to enhance understanding of atherosclerotic plaque formation and progression. Also, the effect of geometric change at the bifurcation area was explored, and it was discovered that this location causes considerable vortex forming zones. The evident velocity reduction and backflow development were seen, reducing shear stress. The findings indicate that low TAWSS < 0.4 Pa and OSI > 0.15 areas within the bifurcation region are more susceptible to atherosclerosis development. Full article

Whole-genome sequencing followed by comprehensive genomic analyses was used to characterize 16 Salmonella isolates from water-overlying sediments in Conococheague Creek (PA), an agricultural irrigation water source. Our goal was to characterize the genomic profiles and diversity of these Salmonella isolates. We identified eight distinct serotypes, including Newport, the most prevalent (43.8%), providing environmental context relevant to agricultural water systems. Genomic surveys showed various Salmonella Pathogenicity Island (SPI) profiles. Although widespread antimicrobial resistance (AMR) genes were not detected, the consistent presence of the aac(6’)-Iaa gene across all isolates and a parC (T57S) mutation in 14 isolates were identified as inherent genotypic markers. Six distinct plasmid replicon types were observed in over 60% of isolates. Replicons for IncF and IncI2 plasmids, frequently associated with β-lactamase genes, were found, documenting the presence of mobile genetic elements despite a lack of acquired AMR genes. Restriction-Modification (RM) systems and CRISPR/Cas loci were also detected, suggesting Salmonella genomic plasticity. Our study showed that sediment-associated Salmonella, notably serotype Newport, harbored diverse virulence-associated genomic features. These findings contributed to the genomic baseline for irrigation water quality and food safety. Full article

Eupatorium fortunei Turcz. (E. fortunei), a member of the Asteraceae family, is a widely utilized traditional medicinal herb in China. Historically, it has been employed to treat conditions such as influenza, nausea, anorexia, and various ailments associated with “pathogenic dampness”. To the best of our knowledge, this study presents the first systematic review of recent research on E. fortunei, based on a comprehensive literature search across both Chinese and international databases, including Web of Science, PubMed, SciFinder, and CNKI. The review encompasses its botanical characteristics, traditional applications, phytochemical composition, pharmacological properties, and toxicological profiles. Current research reveals a diverse array of phytochemicals in E. fortunei, with 162 compounds identified to date, including thymol derivatives, terpenoids, alkaloids, benzofurans, fatty acids, and other bioactive constituents. These compounds exhibit a broad spectrum of pharmacological activities, encompassing anti-cancer, anti-viral, anti-fungal, anti-inflammatory, and anti-diabetic effects. Among these, thymol derivatives and benzofurans emerge as the most prominent bioactive compounds, demonstrating potent cytotoxic effects against various tumor cell lines. Although E. fortunei is generally considered safe, certain pyrrolizidine alkaloids (PAs) present potential hepatotoxic risks, which can be mitigated through appropriate dosage control and formulation optimization. As a valuable traditional Chinese medicinal herb, E. fortunei exhibits substantial therapeutic potential. In conclusion, this review provides a comprehensive and systematic overview of current research on E. fortunei, offering scientific evidence and guidance for its rational development and clinical application. Full article

Background/Objectives: The coordination of flowering and dormancy represents a fundamental adaptive strategy for perennial plant survival. Recent studies have demonstrated that AIL1 homologs act as integrators of short-day signals, playing pivotal roles in seasonal growth cessation and dormancy regulation in poplar. Despite these advances, whether AIL1-mediated regulatory mechanisms are conserved in Platanus acerifolia, a species with distinct phylogenetic and ecological characteristics, remains an open question. Methods: In this study, two AIL1 homologs, PaAIL1a and PaAIL1b, were isolated from P. acerifolia. Their biological functions were systematically investigated through sequence analysis, spatiotemporal expression profiling, environmental stress treatments, yeast one-hybrid assays, and luciferase (LUC) transient expression assays. Results: PaAIL1s (PaAIL1a and PaAIL1b) exhibited ubiquitous expression across diverse tissues and organs, functioning as mediators of photoperiod and temperature signaling to orchestrate bud dormancy regulation. Interaction and activation assays placed these factors downstream of PaFUL proteins. While displaying functional redundancy in dormancy induction and floral development, the paralogs underwent distinct subfunctionalization: PaAIL1a specifically regulated flowering initiation and dormancy release, whereas PaAIL1b acted predominantly during the ecodormancy phase. Conclusions: These results establish PaAIL1 genes as critical integrators of environmental signals and developmental programs, governing seasonal growth dynamics in this species. Full article

Super-resolution microscopy has transformed biological imaging by enabling nanoscale visualization of cellular structures beyond the diffraction limit. However, its effective application in highly dense molecular environments still poses challenges. This is the case for 3D PhotoActivated Localization Microscopy (PALM) achieved through astigmatism in bacterial cells. The limited volume of a single bacterium highly increases the probability of the intensity profiles emitted by single chromophores to overlap, thus strongly decreasing the number of localizations, leading to dramatic undersampling. Dual-color 3D super-resolution in Escherichia coli is achieved through a combination of PALM with Expansion Microscopy (Ex-PALM). PALM provides high specificity through photoactivable (PA) fusion proteins and high localization precision, while ExM physically expands the specimen and separate densely packed molecules. This hybrid approach enables dual-color 3D single-molecule localization with about 3 nm spatial resolution, thus allowing one to measure distances down to the molecular scale. This is achieved by optimizing ExM protocols in bacteria to achieve a 4-fold isotropic expansion, by minimizing both chromatic aberrations and signal crosstalk, and by improving single-molecule sensitivity through highly selective inclined illumination. The method is applied to measure the spatial distribution of HisF and HisH proteins, involved in E. coli histidine biosynthesis. By tagging each protein with a photoactivable fluorescent protein, Ex-PALM reveals that after being synthetized, they co-localize in the bacterial volume with an average 3D distance of 19 nm. By combining labeling specificity with Ex-PALM, an effective method is developed for studying molecular organization in prokaryotes and in high-density samples in general, such as cell organelles or molecular condensates, with broad applications in microbiology, synthetic biology, and cellular biophysics. Full article

Boron coatings were deposited by RF magnetron sputtering in an Ar atmosphere at a constant power of 80 W, varying the working pressure in the 0.6–5 Pa range. Plasma diagnostics were performed by means of a Langmuir probe to determine the electron temperature and electron density under different operating conditions. Within the investigated pressure range, the deposition rate remained nearly constant, whereas a significant decrease in coating mass density was observed with increasing pressure. The coatings display a columnar structure at all investigated pressures, with no significant differences in bulk morphology. Pressure primarily affects the surface features, leading to an increase in the density, lateral dimensions, and height of surface agglomerates with increasing pressure. Compositional analysis by EDX revealed a substantial oxygen incorporation in the films, with the lowest oxygen content (~11 at.%) measured for the coating deposited at 0.6 Pa. XPS depth profiling confirmed the presence of oxygen and evidenced the formation of boron oxide species, while the boron concentration exceeded 80 at.% in all samples. These results highlight the strong sensitivity of boron film density and oxygen uptake to sputtering pressure. Full article

With the rising prevalence of feline kidney diseases, effective preventive and therapeutic strategies are urgently needed. This study evaluated the effects of Cybister chinensis extracts (CCME) and Periplaneta americana residue extracts (PAE) on inflammation-associated, oxidative stress-related, and fibrosis-related responses in Crandell-Rees Feline Kidney (CRFK) cells. Using MTT assays, flow cytometry, and qPCR, we assessed cytoprotection in models of lipopolysaccharide (LPS)-, hydrogen peroxide (H₂O₂)-, and palmitic acid (PA)-induced injury. Preliminary HPLC fingerprint analysis of three batches of a combined extract from Periplaneta americana residues and Cybister chinensis Motschulsky (CPCE) revealed similar chromatographic profiles, indicating good batch-to-batch consistency. Within non-cytotoxic ranges, CPCE increased cell viability and reduced apoptosis in injured CRFK cells. Anti-inflammatory effects were evidenced by significant downregulation of TNF-α and IL-6 mRNA. Potential antioxidant-related effects were suggested by decreased expression of oxidative stress–responsive genes SOD1, CAT, and GSTP1. In the PA model, anti-fibrotic potential was supported by reduced TGFB1 expression, accompanied by improvements in inflammatory and oxidative stress markers, and by decreased levels of fibrosis-associated markers α-SMA, COL I, and HCB III. These findings suggest that CPCE exerts cytoprotective effects in vitro, potentially through modulation of inflammation, oxidative stress, and fibrosis. Full article

Background: To mitigate the negative impacts of the pandemic, it is essential to understand how the associations between different types of physical activity (PA) and adolescent well-being changed before and after the COVID-19 pandemic (defined here as the period marked by students’ return to stable in-person education). This study aimed to examine gender differences in the associations between school-related PA and subjective well-being before and after the pandemic. Methods: A cross-sectional design was used, including 430 boys and 571 girls from 22 high schools. Participants completed the Youth Activity Profile questionnaire to assess school-related and school-associated PA and the WHO-5 Well-Being Index to evaluate subjective well-being. Differences in participants’ PA across segments of the school day before and after the pandemic were evaluated using the Kruskal–Wallis test, and compliance with PA recommendations was analyzed using cross-tabulation and Pearson’s chi-square tests. Results: After the pandemic, both boys and girls reported significantly lower levels of active transportation to and from school compared with the pre-pandemic period. In addition, well-being levels were significantly lower in both genders after the pandemic. Before the pandemic, boys and girls with higher well-being met the recommendations for PA to school, from school, and outside of school significantly more often than their peers with lower well-being. Higher levels of well-being were observed both before and after the pandemic in boys and girls who participated in organized PA compared with non-participants. Conclusions: This study confirms lower levels of PA and well-being among adolescents after the pandemic. In particular, PA to and from school was at a lower level after the pandemic than before the pandemic. Participation in organized PA was significantly associated with higher well-being in both boys and girls before and after the pandemic. Supporting adolescents’ participation in organized PA should be a priority when addressing the negative consequences of societal crisis situations. Improved knowledge of the associations between PA and well-being may contribute to more effective support for adolescents’ PA and greater awareness of the importance of meeting PA recommendations. Full article

Background/Objectives: Youths with cerebral palsy (CP) have reduced levels of physical activity (PA) due to motor impairments and functional difficulties. Few studies have observed its link with various factors and none in young adults with CP. This study aimed to investigate the relationships between PA and various factors in young adults with CP, such as gait function, endurance, participation, and personal/environmental influences. Methods: Participants over 15 years old with CP who underwent Clinical Gait Analysis (CGA), the 6 min walk test, and wore an actimeter (ActiGraph GT3X+) for seven days were included. PA was assessed by daily step count (NbSteps/day). Explanatory factors included the Gait Profile Score (GPS), walking speed, subjective walking perception, joint pain, fatigue, 6 min walk distance, SF-36 questionnaire scores, and lifestyle habits. Correlations, univariate, and multivariate regression models were used for the analysis. Results: Forty-seven CP patients (28 males, 19 females, mean age 23.6 years) were included, with 82% classified as GMFCS I and 18% as GMFCS II. The average NbSteps/day was 5685. Significant correlations were found between NbSteps/day and subjective perception, pain, GMFCS level, and walking speed. Multivariate regression identified walking speed and physiotherapy (PT) sessions as significant predictors of PA. Conclusions: PA in young adults with CP is linked to walking speed, GMFCS level, joint pain, fatigue, and PT. No differences have been observed between patient unilateral or bilateral CP. However, individual behaviors vary and are not fully explained by linear regression analysis. Full article

Propagating easterly waves (EW) are analyzed here, within the dynamical environment of the Caribbean Low-Level Jet (CLLJ) using radiosondes from the Organization of Tropical East Pacific Convection (OTREC) field campaign, ERA5 reanalysis, and lightning from the World Wide Lightning Location Network (WWLLN) over $5^{\circ }$–${20}^{\circ }$ N, ${60}^{\circ }$–${100}^{\circ }$ W during 21 August–30 September 2019. Radiosondes resolve the vertical structure of the waves at San Andrés (Colombia), Limón and Santa Cruz–Guanacaste (Costa Rica), while ERA5 provides spatial–temporal continuity and vertically integrated diagnostics—namely, the vertically integrated moisture flux divergence (VIMFD) and the vertically integrated geopotential flux divergence (VIGFD). Lightning from WWLLN and precipitation from ERA5 and the Integrated Multi-satellite Retrievals for the Global Precipitation Measurement mission (GPM IMERG) offer independent convective proxies to track disturbances. Mean profiles from radiosondes and ERA5 show strong agreement at Limón and Guanacaste and some differences at San Andrés, yet all datasets capture coherent, phase-locked anomalies in zonal wind, meridional wind, temperature, humidity, vertical velocity and vorticity used to diagnose EW–CLLJ interactions. VIMFD, VIGFD, lightning and precipitation exhibit westward-propagating cores that align with the above anomalies, indicating that organized convection is coupled to the disturbances, whereas the mean state preconditions the environment to enable wave-induced upward motion. A robust vertical adjustment of the CLLJ is documented: the core shifts from near 925 hPa over the Caribbean Sea to about 700 hPa over the Eastern Tropical Pacific ($\Delta p\sim 150$ hPa). This feature is reproduced by a 30-year ERA5 climatology, consistent with jet-exit forcing and enhanced boundary-layer coupling over land. Conditions favorable for barotropic instability using the Rayleigh–Kuo criterion, were present over most of the period. A qualitative barotropic conversion proxy, computed from the eddy momentum covariance $⟨u^{\prime }{v}^{\prime }⟩$, shows positive values in the lower troposphere at Guanacaste and in the layer 850–700 hPa at San Andrés, suggesting mean-to-eddy momentum transfer, whereas the signal at Limón is weaker. Together, these results provide a physically consistent view of EW–CLLJ interactions across the IAS; therefore, a schematic of those mechanisms is proposed here. The results highlight the need for high-resolution modeling and full energy-budget analyses. Full article

Background: Assessing aortic dissection (AD) in its early stages is crucial for cardiovascular surgeons to improve patient outcomes and avoid complications associated with surgical intervention for type A aortic dissection. Initial evaluations rely on patient referrals for computed tomography (CT) scans, which involve measuring the maximum aortic diameter. Objective: This study aimed to improve current diagnostic thresholds for type A aortic dissection by using computational fluid dynamics (CFD) modeling to correlate hemodynamic factors related to the wall shear stress with maximum aortic diameter growth rate, offering insights into predicting AD progression and reassessing current diameter-based diagnostic criteria. Methods: The pre- and post-AD scan data, with an average duration of three and a half years for the 15 patients, were converted into 3D geometries. These geometries were analyzed using the transitional-turbulent CFD model. Wall shear stress (WSS), its derivatives, and the pressure gradient from the pre-AD CT scans were compared across 15 patients, grouped according to the aortic diameter growth per year. Results: For patients in group 1 (nine patients with normal diagnosis), pre-AD time-average wall shear stress (TAWSS) was mostly 2–4 Pa, above physiologic levels. Post-AD, values dropped below 1.5 Pa (stagnant, thrombus-prone), with oscillatory shear index (OSI) elevated (0.24–0.32). In group 2 (n = 6, abnormal diagnosis), post-AD TAWSS was <3 Pa (thrombosis risk), with OSI 0.1–0.31 near tear sites. These findings confirm a dual-risk profile: low TAWSS promotes thrombosis, while high TAWSS drives dissection progression. Conclusions: WSS parameters, such as TAWSS and OSI, can be utilized to assess the development of a dilated ascending aorta, especially for extreme maximum aortic diameter. Pre-AD analysis for some patients revealed a strong negative correlation, indicating that high shear stress in the true lumen (TL) results in a drop in diastolic pressure post-AD at the upward-going section of the aorta. Full article

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with a 5-year survival rate of 13.3%. First-line treatment relies on two chemotherapy regimens, FOLFIRINOX (FOLFNX) or gemcitabine plus nab-paclitaxel (GEMPAC). However, direct clinical comparisons between these regimens have yielded inconsistent results across survival and toxicity endpoints, and the molecular basis of heterogeneous treatment responses remains poorly defined. To investigate regimen-specific tumor-cell-intrinsic mechanisms, we performed quantitative proteomic profiling of a primary PDAC-derived MIA PaCa-2 cell line following treatment with FOLFNX or GEMPAC. Differentially expressed proteins were analyzed using Gene Ontology, KEGG, and Ingenuity Pathway Analysis to define pathway-level alterations, and findings were contextualized using TCGA transcriptomic data. Proteomic analyses revealed that FOLFNX and GEMPAC engage in distinct cytotoxic programs. FOLFNX predominantly suppressed ribosome biogenesis and mitochondrial translation, consistent with sustained metabolic and biosynthetic stress, whereas GEMPAC preferentially disrupted mitotic cytokinesis and phosphatidylinositol phosphate biosynthesis, consistent with mitotic failure. Integration with TCGA data showed that FOLFNX-altered proteins aligned with favorable prognostic expression signatures, whereas GEMPAC-associated proteins were enriched among adverse profiles, reflecting engagement of distinct tumor-intrinsic programs. Together, these findings provide mechanistic insight into differential chemotherapy responses and establish a foundation for proteomics-based biomarkers to guide personalized chemotherapy selection in PDAC. Full article