Search Results (13,228)

The development of highly sensitive approaches for detecting tumor cells in biological samples remains a critical challenge in laboratory and clinical oncology. In this study, we investigated the structural and magnetic properties of iron oxide nanoparticles incorporated into cellulose microspheres of two size ranges (~100 and ~700 μm) and evaluated their potential for targeted tumor cell isolation. In the smaller microspheres, magnetite-based magnetic nanoparticles (MNPs) were synthesized in situ via co-precipitation, whereas pre-synthesized MNPs were embedded into the larger microspheres. The geometrical characteristics of the resulting magnetic cellulose microspheres (MSCMNs) were assessed by confocal microscopy. Transmission electron microscopy and X-ray diffraction analyses revealed an average magnetic core size of approximately 17 nm. Magnetic properties of the MNPs within MSCMNs were characterized using a highly sensitive nonlinear magnetic response technique, and their dynamic parameters were derived using a formalism based on the stochastic Hilbert–Landau–Lifshitz equation. To evaluate their applicability in cancer diagnostics and treatment monitoring, the MSCMNs were functionalized with a TKD peptide that selectively binds membrane-associated Hsp70 (mHsp70), yielding TKD@MSCMNs. Magnetic separation enabled the isolation of tumor cells from biological fluids. The specificity of TKD-mediated binding was confirmed using Flamma648-labeled Hsp70 and compared with control alloferone-conjugated microspheres (All@MSCMNs). The ability of TKD@MSCMNs to selectively extract mHsp70-positive tumor cells was validated using C6 glioma cells and mHsp70-negative FetMSCs controls. Following co-incubation, the extraction efficiency for C6 cells was 28 ± 14%, significantly higher than that for FetMSC (7 ± 7%, p < 0.05). These findings highlight the potential of TKD-functionalized magnetic cellulose microspheres as a sensitive platform for tumor cell detection and isolation. Full article

Background and aims: Reward-based eating reflects hedonic drivers of intake, including loss of control, diminished satiety, and preoccupation with food. We translated, adapted and studied the psychometric properties of the 13- and 5-item Reward-Based Eating Drive Scale (RED), for Portugal, Poland and Italy. Methods: A cross-cultural study was conducted with higher education students and general population samples (n = 1999). After translation and cultural adaptation, the RED was administered with food craving items, and collection of sociodemographic and anthropometric data. Factorial structure and measurement invariance were tested using confirmatory factor analysis (CFA), internal consistency with Cronbach’s alpha, and convergent validity via correlations with BMI and cravings. Results: CFA supported the expected structures of the RED-13 (three factors) and RED-X5 (unifactorial), with configural and metric invariance across countries and groups. Only partial scalar invariance was achieved for both versions. The RED-13 showed good to excellent internal consistency for total scores (0.868 ≤ α ≤ 0.906), with acceptable to good reliability for Loss of control (0.769 ≤ α ≤ 0.821), lower values for Lack of satiety (0.655 ≤ α ≤ 0.723), and good to excellent consistency for Preoccupation with food (0.881 ≤ α ≤ 0.918). The RED-X5 showed acceptable internal consistency (0.737 ≤ α ≤ 0.811) and correlated strongly with RED-13 (r = 0.949, p < 0.001). Both correlated positively with BMI and food cravings. Age, sex, and country had small to medium multivariate effects on RED scores. Conclusions: The RED-13 and RED-X5 showed good psychometric properties in Portugal, Poland, and Italy, with the RED-13 providing a multifactorial assessment and the RED-X5 offering a brief alternative. Full article

Background: Scleral fixation of intraocular lenses (IOLs) is a valuable option in cases of aphakia or inadequate capsular support, yet conventional sutured and sutureless approaches can pose technical challenges and complication risks. The needle-guided scleral fixation technique offers a simplified, single-suture solution that enhances safety and reproducibility. Methods: In this retrospective interventional case series, 30 eyes with insufficient capsular support underwent IOL implantation using Meduri’s needle-guided single-suture technique at the G. Martino University Hospital, Messina. The surgical method employs a 24-gauge needle to guide a double-armed 10-0 polypropylene suture through the sclera for precise IOL anchorage, minimizing vitreous manipulation. Outcomes were assessed over 24 months, including best-corrected visual acuity (BCVA), IOL centration, intraocular pressure (IOP), and postoperative complications. Results: Mean BCVA improved from X to Y LogMAR at two years (p < 0.05). All IOLs remained well-centered without tilt or decentration. Mild conjunctival hyperemia occurred in 70% of cases, resolving spontaneously. No suture erosion, vitreous hemorrhage, or retinal detachment was observed. Conclusions: The needle-guided single-suture technique provides a stable, efficient, and reproducible method for posterior chamber IOL fixation in aphakic eyes lacking capsular support. Its minimal learning curve and reduced surgical complexity make it an attractive alternative to both traditional sutured and modern sutureless methods, particularly in centers without vitreoretinal expertise. Full article

Anthonoic acids A–C (1–3), the first representatives of sulfated and N-(2-hydroxyethyl)-substituted lipidic α-amino acids, were isolated along with their plausible precursor, anthamino acid A (4), from the marine sponge Antho ridgwayi. The structures of these compounds were determined using the analysis of 1D and 2D NMR and HR ESI mass spectra. A structural feature of 1–4, compared to all previously known lipidic amino acids, is the presence of a sulfate group near the end opposite the amino acid terminus. At a concentration of 1 µM, anthonoic acids A–C (1–3) effectively protected H9c2 and SH-SY5Y cells in biotests, which modeled hypoxia induced by the addition of CoCl₂ to the medium and damage caused by ischemia/reperfusion. These natural products act via the Nrf2-mediated pathway by reducing intracellular ROS levels, accompanied by the upregulation of SOD activity, which is controlled by the Nrf2 transcriptional factor. Anthonoic acids A–C (1–3) do not activate the transcriptional activity of NF-κB but inhibit ATP-induced cell damage and calcium influx, indicating the involvement of P2X7 receptors in the cytoprotective effect of anthonoic acids A–C. Full article

Mutations in the POU1F1 gene cause defects in the expression of the genes encoding thyroid-stimulating hormone (TSH)-β subunit, growth hormone (GH), and prolactin (PRL). Here, we characterized 15 missense and nonsense mutations. Protein stability was reduced in the P14L, P24L, F135C, K145X, F233S and E250X mutants. Transactivation by 15 mutants in the TSHβ promoter was moderately correlated with that of the PRL promoter. Based on their transcriptional activity, we classified them into three groups: group I, equivalent to the wild type; group II, partial; and group III, substantially lost. A review of case reports on four patients with group II mutations revealed that TSH deficiency manifested after recombinant GH therapy. A transcription factor, GATA2, is the main activator in the TSHβ gene, while POU1F1 protects its function from inhibition by the suppressor region (SR). We found that the SR is critical for the pathogenesis of TSH deficiency. The transactivation of the TSHβ promoter by the K216E mutant was equivalent to that of wild-type POU1F1; however, that of the PRL promoter was low, while the opposite was found in the R271W mutant. The functional property of K216E suggests that the interaction of POU1F1 with GATA2 may not always be necessary for the activation of the TSHβ promoter. Full article

Background/Objectives: Posterior tibial slope (PTS) is an established risk factor for anterior cruciate ligament (ACL) injury in adults. In pediatric population, this relation is less established, and the PTS measurement is not clearly defined. To determine the agreement between X-ray (XR) and magnetic resonance imaging (MRI) PTS measurements and to establish an MRI cutoff corresponding to the standard radiographic ≥12° definition of “high slope”. Methods: In this retrospective study, 108 adolescent knees with ACL rupture underwent paired XR and MRI evaluation by two reviewers. Agreement was assessed with Pearson and Spearman correlation, intraclass correlation coefficient (ICC), Bland–Altman analysis, and Deming regression. Diagnostic performance of MRI thresholds was compared with XR ≥ 12° as reference. Results: Mean PTS was higher on XR (10.2 ± 3.1°) than on MRI (8.4 ± 2.8°), with a systematic bias of +1.8° revealed by Bland–Altman analysis. These two measurements showed strong positive correlation (r = 0.602, p < 0.001) and moderate concordance (ICC = 0.506, 95% CI, 0.186–0.696, p = 0.0015). Individual differences ranged up to ±5° between modalities. Using XR ≥ 12° as reference for “high slope,” ROC analysis identified an optimal MRI cutoff of 8.8° with excellent diagnostic accuracy (AUC = 0.841, 95% CI, 0.760–0.922). Conclusions: Radiographic measurements systematically overestimate PTS relative to MRI. Numeric thresholds are not interchangeable between modalities. An MRI cutoff of approximately 9° corresponds to the radiographic ≥12° definition of high slope and may serve as a pragmatic reference for interpreting MRI-based measurements in pediatric patients, requiring further validation. Full article

The emergence and spread of mobile colistin resistance (mcr) genes pose a significant challenge in controlling multidrug-resistant Gram-negative pathogens. Understanding the epidemiology of mcr-carrying plasmids is essential for mitigating their dissemination across humans, animals, and the environment. To characterize their spatiotemporal dynamics on a global scale, we analyzed an extensive collection of 5,549 mcr-carrying plasmids spanning 1995 to the present. We found that cross-genera transmission patterns of mcr-carrying plasmids varied across four distinct periods. Initially, IncHI2/HI2A plasmids provided a survival advantage across genera and regions, followed by IncI2, and ultimately by IncX4. Moreover, the three plasmid lineages (i.e., IncX4, IncI2, and IncHI2/HI2A) have reached a stable distribution across diverse bacterial hosts and geographic regions through horizontal gene transfer and clonal expansion. By integrating sequence similarity clustering of plasmids and mcr-related genetic environments, we identified 79 cross-genus, 43 intra-E. coli, and 10 intra-S. enterica transmission units. Molecular dating analysis traced the origin of IncX4 plasmids to 1990 in animal hosts, with phylogenetic evidence indicating potential cross-host, -genus, and -region exchange. Notably, IncP1 plasmids emerged as important vectors of mcr-1 and mcr-3 spread, particularly in Southeast Asia, warranting enhanced surveillance. These findings provide critical insights into the global transmission networks of plasmid-mediated mcr genes and underscore the urgent need for coordinated interventions. Full article

Accurate and efficient forest inventory methods are crucial for monitoring forest ecosystems, assessing carbon stocks, and supporting sustainable forest management. Traditional field-based techniques, which rely on manual measurements such as diameter at breast height (DBH) and tree height (TH), remain labour-intensive and time-consuming. In this study, we introduce and validate a fully open-source, low-cost terrestrial laser scanning system (LCA-TLS) built from commercially available components and based on the Livox Avia sensor. With a total cost of €2050, the system responds to recent technological developments that have significantly reduced hardware expenses while retaining high data quality. This trend has created new opportunities for broadening access to high-resolution 3D data in ecological research. The performance of the LCA-TLS was assessed under controlled and field conditions and benchmarked against three reference devices: the RIEGL VZ-1000 terrestrial laser scanner, the Stonex X120GO handheld mobile laser scanner, and the iPhone 15 Pro Max structured-light device. The LCA-TLS achieved high accuracy for estimating DBH (RMSE: 1.50 cm) and TH (RMSE: 0.99 m), outperforming the iPhone and yielding results statistically comparable to the Stonex X120GO (DBH RMSE: 1.32 cm; p > 0.05), despite the latter being roughly ten times more expensive. While the RIEGL system produced the most accurate measurements, its cost exceeded that of the LCA-TLS by a factor of about 30. The hardware design, control software, and processing workflow of the LCA-TLS are fully open-source, allowing users worldwide to build, modify, and apply the system with minimal resources. The proposed solution thus represents a practical, cost-effective, and accessible alternative for 3D forest inventory and LiDAR-based ecosystem monitoring. Full article

Background/Objectives: Accurate full-arch implant impressions are essential for predictable digital prosthodontics, yet the performance of different intraoral scanners (IOSs) remains variable. This in vitro study compared the trueness and precision of three widely used IOSs-Sirona Primescan, 3Shape TRIOS Core, and Medit i700-in a standardized full-arch implant model. Methods: A maxillary model with six multi-unit implants was digitized using a high-accuracy laboratory scanner to obtain the reference dataset. Each IOS was used to perform ten scans, exported as unmodified STL files. Accuracy was evaluated in Geomagic Control X through a two-step alignment and a peri-implant region-of-interest deviation analysis. Trueness (mean absolute surface deviation, µm) and precision (SD) were compared using one-way ANOVA with Tukey’s test (α = 0.05). Results: Primescan and TRIOS Core showed comparable trueness (202.76 ± 13.89 µm and 204.21 ± 2.61 µm, respectively), while Medit i700 demonstrated significantly higher deviations (221.05 ± 6.28 µm) (p < 0.05). TRIOS Core exhibited the highest reproducibility across repeated scans. Conclusions: The three scanners demonstrated measurable accuracy differences under standardized conditions. Primescan and TRIOS Core performed similarly in trueness, with TRIOS Core achieving superior precision. Medit i700 showed higher deviation values but remained consistent in its performance. These findings highlight measurable differences in accuracy and reproducibility among intraoral scanners under standardized laboratory conditions and may assist clinicians in selecting appropriate devices for full-arch digital implant workflows; however, clinical validation is required to confirm their performance in vivo. Full article

In this work, a layout-level design methodology is presented for Low-Noise Amplifiers (LNAs), targeting a wide frequency spectrum from RF to millimeter-wave (mmWave) bands, and implemented using a 22 nmFDSOI CMOS process. A nested inductor structure is introduced at RF frequencies to reduce silicon footprint, with magnetic crosstalk effects characterized through electromagnetic (EM) simulations using Ansys^® RaptorX, Release 2024 R2, ANSYS, Inc. and integrated into the design process. Single-ended LNA architectures are employed for RF bands, while at mmWave frequencies, a differential topology is adopted to enhance linearity and enable simultaneous input and output impedance matching. An EM-based verification flow is applied across all designs to ensure RF/mmWave design flow compatibility, simulation accuracy, and enhanced performance. The proposed designs are evaluated using key metrics including input/output matching, reverse isolation, forward gain, noise figure, linearity ($I{P}_1,I{P}_3$), stability factor, power consumption, and total chip area to quantify the efficiency of the proposed methodology. The simulation results demonstrate that nested inductors are highly effective for area reduction in RF LNAs, while differential topologies are more suitable for mmWave designs, providing a unified framework for area-efficient and high performance LNA implementation. Full article

In this research, we sought to methodically examine the protective effects of Gastrodia elata extract (GEE) on liver damage induced by D-galactose (D-gal) in mice and clarify the underlying mechanisms. The chemical composition of GEE was characterized using Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry (UPLC-MS/MS), while network pharmacology analysis was employed to predict potential molecular targets and signaling pathways. A mouse model of liver injury was established through daily intraperitoneal injection of D-gal over a 42-day period, during which the hepatoprotective efficacy of GEE was evaluated. Biochemical, histopathological, and molecular analyses were subsequently performed. UPLC-MS/MS identified ingredients such as amino acids, aromatic compounds, fatty acids, and terpenoids in GEE. A network pharmacology analysis enabled the identification of 272 common targets linked to GEE and liver damage, demonstrating notable enrichment within the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway. In vivo experiments demonstrated that GEE effectively alleviated D-gal-induced body weight loss and elevated liver index values, alleviated hepatic histological damage, and reduced serum levels of Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), and Alkaline Phosphatase (ALP). Furthermore, GEE enhanced the activities of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), decreased malondialdehyde (MDA) levels, and downregulated the mRNA expression of the pro-inflammatory cytokines Interleukin-6 (IL-6), Interleukin-1 beta (IL-1β), and Tumor Necrosis Factor-alpha (TNF-α). Western blot analysis confirmed that GEE activated the PI3K/Akt pathway, as evidenced by increased ratios of phosphorylated Phosphatidylinositol 3-kinase/Phosphatidylinositol 3-kinase (p-PI3K/PI3K) and phosphorylated AKT/Protein Kinase B (p-AKT/AKT); restored the B-cell lymphoma 2-associated X protein/B-cell lymphoma-2 (Bax/Bcl-2) balance; and reduced cyclin-dependent kinase inhibitor 1 (p21) expression. The results suggest that GEE protects against D-gal-induced liver damage by reducing oxidative stress, inhibiting inflammatory responses, and modulating apoptosis through the activation of the PI3K/Akt signaling pathway, providing support for its potential use in hepatoprotection. Full article

This study evaluated the push-out bond strength (PBS) and failure modes of fiber posts cemented with silane-containing self-adhesive resin cement (SARC) compared with conventional SARC and universal adhesive strategies, considering the effects of root section and aging. Ninety single-rooted human premolars were equally assigned to three cementation protocols: silane-containing SARC (PANAVIA SA Cement Universal), conventional SARC (RelyX Universal), and universal adhesive plus SARC (Scotchbond Universal Plus + RelyX Universal). Each group was divided into two aging subgroups: 24 h water storage and thermal cycling (10,000 cycles between 5 °C and 55 °C, 30 s dwell time; n = 15). After root canal treatment and post space preparation, glass fiber posts were cemented, and each root was sectioned to obtain six slices. PBS was measured using a push-out test, and failure modes were examined under stereomicroscopy. Data were analyzed using three-way ANOVA, post hoc tests, Spearman’s correlation, and logistic regression (α = 0.05). Cement type, root section, and aging significantly influenced PBS (p < 0.001). PBS decreased from coronal to apical sections, and thermal cycling reduced PBS in all groups. The universal adhesive plus SARC achieved the highest PBS, while conventional SARC had the lowest PBS. Cementdentin adhesive failures (FM2) predominated overall, with proportions varying between 43% and 90%, and higher PBS values were associated with fewer FM2 failures. The combination of a universal adhesive with SARC provided superior bonding compared to simplified protocols. Although silane-containing SARC improved bonding relative to conventional SARC, durable adhesion to radicular dentin remains challenging, particularly in apical regions. Full article

Background: Sustainable diets, particularly the Mediterranean diet, are associated with improved human and planetary health. Nutrition literacy influences food choices, yet intergenerational differences in literacy and adherence to sustainable diets remain underexplored. This study examined the relationship between nutrition literacy, adherence to the Mediterranean diet, and sustainable nutrition behaviors among Generation X, Y, and Z adults living in Istanbul. Methods: A cross-sectional online survey was conducted among adults aged 18–60 years. Data were collected through a demographic questionnaire, the E-Healthy Nutrition Literacy Scale, and the Short Questionnaire for Mediterranean Diet Adherence and Sustainable Nutrition. Parametric statistical analyses were performed. Results: Among 1395 participants, adherence to the Mediterranean diet did not significantly differ by generation or gender (p > 0.05). Participants with prior nutrition education were significantly more likely to achieve high nutrition literacy scores and adhere to sustainable eating practices. Adherence to sustainable nutrition increased across generations, with Generation Z exhibiting the highest proportion of low adherence (53%), while Generations Y and X demonstrated progressively higher rates of moderate (39%) and high adherence (31.6%) (p < 0.05). Correlation analyses indicated that adherence to sustainable nutrition was positively associated with the application dimension of nutrition literacy, suggesting that both knowledge and practical skills may influence sustainable nutrition behaviors. Conclusion: Older and more educated participants achieved higher nutrition literacy and sustainable nutrition adherence scores. Knowledge alone is insufficient to ensure healthy or sustainable eating. Enhancing practical skills and providing sustainability-focused nutrition education may improve diet quality and public health. Full article

Alzheimer’s disease (AD) is marked by cognitive decline, and also by retinal degeneration. Having in mind that docosahexaenoic acid (DHA, 22:6n − 3) is a safe, low-cost, and pivotal fatty acid for brain health and sustained cognitive function, this study exploits environmentally friendly non-fish sources as potential dietary supplements enriched with DHA to prevent or reverse AD. Forty 5xFAD transgenic male mice, aged five weeks old, were randomly distributed by five body weight-matched dietary groups (with eight animals each) and fed isocaloric diets based on the AIN-93M standard formulation for rodents for 6 months. Except for the control feed (without supplementation), each diet contained a modified lipidic fraction supplemented with 2% of the following: (1) linseed oil (LSO, rich in alpha-linolenic acid (ALA, 18:3n − 3)); (2) cod liver oil (fish oil, FO, rich in both DHA and eicosapentaenoic acid (EPA, 20:5n − 3)); (3) Schizochytrium sp. microalga oil (Schizo, with 40% of DHA); and (4) commercial DHASCO (DHASCO, with 70% of DHA). The aim of this study was to measure retinal neural layer thickness, calculate ganglion cell layer (GCL) density, and assess retinal injury by means of immunohistochemical staining for β-amyloid plaques deposition, TAU protein levels, and IBA1, as hallmark features of AD progression, in order to elucidate the effects of different dietary DHA treatments in Alzheimer’s retinas. Although no statistical differences were observed across retinal layer thicknesses depending on the diet (p > 0.05), there was a consistent pattern for slightly increased retinal thickness in 5xFAD mice fed fish oil relative to the others for the measurement of total layers, in general and for the inner segment/outer segment layer, the outer nuclear layer, the outer plexiform layer, the inner nuclear layer, and the inner plexiform layer, in particular. The ganglion cell layer (GCL) density was increased in 5xFAD mice fed the DHASCO oil diet relative to the control (p < 0.05), suggesting a benefit of DHA supplementation on the number of viable ganglion cells. No positive staining was observed for β-amyloid plaques deposition or the neuroinflammatory marker, IBA1, corroborating previous findings in human AD retinas. Conversely, the internal retinal layers showed intense TAU immunostaining. Immnunostained TAU area was significantly reduced in 5xFAD mice fed a fish oil diet compared to control (p < 0.05), although the number of TAU-positive cells did not differ across diets (p > 0.05). The retinal protected integrity derived from the benefits of DHA supplementation found, either from fish oil or DHASCO oil, underscores the potential of retinal biomarkers as non-invasive indicators of cognitive decline and overall brain health, opening new avenues for investigating AD pathophysiology in the retina. Full article

Vegetation concrete is a composite material integrating plant growth and concrete technology. In this study, solid waste materials (phosphogypsum and recycled aggregates) were utilized to prepare vegetation concrete. Semi-hydrated phosphogypsum (HPG) was used to replace ordinary Portland cement as a cementitious material in a gradient manner, while recycled coarse aggregates (RCAs) fully replaced natural crushed stone. The basic properties of phosphogypsum–recycled aggregate-based vegetation concrete were analyzed, and X-ray diffraction (XRD) and scanning electron microscopy (SEM) were employed to characterize the hydration products of vegetation concrete with different mix ratios. The results indicated that replacing cement with HPG exerted a significant alkali-reducing effect and provided favorable cementitious strength. When the porosity was 24% and the HPG content was 50%, the vegetation concrete exhibited optimal performance: the 28-day compressive strength reached 12.3 MPa, and the pH value was 9.7. Recycled aggregates had a minimal impact on strength. When 0.5% sodium gluconate was added as a retarder, the initial setting time was 97 min and the final setting time was 192 min, which met construction requirements with little influence on later-stage strength. Microscopic analysis revealed that the early strength (3d–7d) of vegetation concrete was primarily contributed by CaSO₄·2H₂O crystals (the hydration product of HPG), while the later-stage strength was supplemented by C-S-H (the hydration product of cement). Planting tests showed that Tall Fescue formed a lawn within 30 days; at 60 days, the plant height was 18 cm and the root length was 6–8 cm. Some roots grew along the sidewalls of concrete pores and penetrated the 5 cm thick vegetation concrete slab, demonstrating good growth status. Full article