Search Results (495)

Due to hardware limitations of Thermal InfraRed (TIR) cameras, TIR images captured by Unmanned Aerial Vehicles (UAVs) suffer from Low Resolutions (LRs) and blurred textures. Improving the spatial resolution of TIR images is of great significance for subsequent applications. Existing image Super-Resolution (SR) methods rely on High-Resolution (HR) ground truth for supervised training, resulting in limited generalization and a lack of mechanisms to preserve the physical consistency of thermal radiation. To address these two issues, this paper proposes an unsupervised super-resolution framework for UAV TIR imagery that integrates diffusion modeling with cross-modal texture transfer. The diffusion model enables stable reconstruction of the fundamental TIR structure without requiring high-resolution supervision, while multi-scale textures extracted from visible (VIS) imagery via Multi-Stage Decomposition based on Latent Low-Rank Representation (MS-DLatLRR) compensate for missing details. To suppress temperature distortions introduced by cross-modal texture transfer, a physics-guided constraint termed Prior-Informed Emissivity-Guided Coefficient Mapping (PI-EGCM) is incorporated. Emissivity-aware guidance maps constructed via semantic classification regulate texture transfer and preserve thermal radiation consistency. Experimental results demonstrate that the proposed method improves spatial resolution and perceptual quality while effectively maintaining temperature fidelity, achieving a balanced enhancement of structural detail and physical consistency. Full article

Purpose: To investigate the mechanisms by which plasma extracellular vesicles (EVs) from preterm infants with bronchopulmonary dysplasia (BPD) elicit inflammation and abnormal angiogenesis in neonatal mouse retinas. Methods: EVs from the plasma of 7-day-old preterm infants, born between 23^0/7 and 29^6/7 weeks of gestation, with BPD or without BPD (nBPD) at 36 weeks postmenstrual ages, were adoptively transferred into postnatal day 3 (P3) mice via intravenous retro-orbital sinus injection. Inflammation and pathological neovascularization in neonatal mouse retinas were examined by immunohistochemistry of retinal flat mounts for Allograft Inflammatory Factor 1 (AIF1), CD206, or Glial Fibrillary Acidic Protein (GFAP) and isolectin-B4 (IB4) staining on P17. Retinal inflammation-related transcripts were assessed by qRT-PCR. Proteomic profiles of BPD and nBPD EVs were examined by Liquid Chromatograph Mass Spectrometer/Mass Spectrometer (LC-MS/MS) and Gene Set Enrichment Analysis (GSEA). Results: Adoptively transferred EVs from BPD and nBPD infants crossed the blood–retinal barrier (BRB) in recipient mouse pups. BPD-EVs increased retinal activated microglia, Müller cells, and twisted proliferative neovascularization compared to nBPD-EVs. BPD-EVs also elevated retinal transcripts regulating inflammation and angiogenesis, including NOD-, LRR- and pyrin domain-containing protein 3 (Nlrp3), Apoptosis-associated speck-like protein containing a caspase recruitment domain (Asc), Caspase 3 (Casp3), Caspase 8 (Casp8), Gasdermin D (Gsdmd), Il1β, Il6, Aif1, and Vascular endothelial growth factor (Vegf). Proteomics analysis revealed that BPD-EVs had significantly elevated levels of inflammation and angiogenesis-related proteins compared to nBPD-EVs. Conclusions: BPD-EVs promote inflammation and abnormal neovascularization by upregulating genes related to apoptosis and inflammation in neonatal mouse retinas. EV protein profiles suggest that elevated levels of proteins such as Defensin alpha 1B (DEFA1B), Insulin-like growth factor binding protein 2 (IGFBP2), CD5 antigen-like (CD5L), von Willebrand factor (vWF), and Tenascin C (TNC) in BPD-EVs may contribute to the observed inflammation and angiogenesis. Full article

F-box-LRR (FBXL) proteins are crucial components of the SCF ubiquitin ligase complex, regulating diverse processes such as development and stress responses in plants. However, the FBXL family in tobacco (Nicotiana tabacum L.) remains poorly characterized. This study performed the first genome-wide analysis of the FBXL gene family in tobacco and identified 47 NtaFBXL genes. Phylogenetic analysis classified them into five clades, among which Clade III exhibited notable expansion. Promoter analysis revealed abundant stress- and hormone-related cis-elements. Expression profiling demonstrated tissue-specific patterns and strong responses to drought, ABA, IAA, and TMV infection. Importantly, six genes exhibited a significant negative correlation with TMV accumulation, suggesting their potential roles in antiviral defense. Moreover, both drought and TMV stress triggered a disturbance of redox homeostasis, a dynamic process that was closely associated with the expression of specific NtaFBXL genes, characterized by upregulated antioxidant enzymes (SOD, POD, CAT) and accumulated oxidative markers (H₂O₂, MDA). Collectively, this study provided a foundational resource for understanding the function of NtaFBXLs and identified key candidate genes for the genetic improvement of stress resistance in tobacco. Full article

Toll-like receptors (TLRs) are essential pattern recognition receptors of the innate immune system and play critical roles in pathogen invasion in teleosts. In this study, we identified and characterized full-length open reading frames of three TLRs belonging to the TLR11 subfamily from Schizothorax prenanti, termed spTLR19 (2868 bp), spTLR20 (2835 bp), and spTLR21 (2946 bp), encoding 955, 944, and 981 amino acids, respectively. All three proteins exhibited the conserved domain architecture typical of TLRs, comprising a leucine-rich repeat (LRR) domain, a transmembrane region, and a Toll/IL-1 receptor (TIR) domain. Phylogenetic and homology analyses revealed that spTLR19 and spTLR20 clustered most closely with their homologues from Cyprinus carpio, while spTLR21 showed the highest similarity to Onychostoma macrolepis TLR21. Expression profiling showed that these TLRs were ubiquitously expressed across examined tissues, with relatively higher expression in immune-related tissues such as spleen and gills. Furthermore, challenge with Streptococcus agalactiae and Aeromonas veronii significantly up-regulated the expression of spTLR19, spTLR20, and spTLR21 in spleen, liver, and gills, suggesting their involvement in antibacterial immune responses. These findings enhance the functional understanding of the teleost TLR11 subfamily and provide a foundation for elucidating disease resistance and immune regulation in S. prenanti. Full article

In this study, a targeted SSR (Simple Sequence Repeat) marker resource was developed based on genes and protein families associated with pathogen- and pest-related defense–resistance mechanisms in Camellia sinensis. Forty-one genes and protein families reported to show upregulation, increased expression, or functional validation under disease and pest stress were selected, and the corresponding 195 loci were mapped onto the Camellia sinensis cv. Shuchazao genome. SSR screening within gene bodies and gene-flanking regions (±5 kb) identified 5197 SSR loci. Putative QTL hotspot regions were defined using locus-based sliding-window analysis, Z-score calculations, and permutation tests, yielding 633 SSRs filtered at the 99% and 95% significance thresholds. Proteome-wide scans based on conserved amino acid motifs identified multiple loci within the WRKY, NAC, LRR, PRX, and CHI families, and Random Forest analysis was used to prioritize SSRs within these families. Finally, 386 SSR primer sets were designed and evaluated by in silico PCR across six tea genomes. Of these, 245 primers produced amplicons in more than one genome, and 124 exhibited polymorphic information content values greater than 0.500. Overall, the developed SSR panels represent a biologically contextualized and experimentally transferable marker resource targeting defense–resistance-associated genic and gene-proximal regions. Full article

Nucleotide-binding and leucine-rich repeat receptors (NLRs) play an important role in plant innate immunity. Previous reports indicate that SUT1 (SUPPRESSOR OF TOPP4 1) is required for the autoimmune response mediated by TYPE ONE PROTEIN PHOSPHATASE 4 (TOPP4) mutation topp4-1 (namely TOPP4^T246M) in Arabidopsis. We observed that co-expression of SUT1 with TOPP4 mutant versions, instead of wild-type TOPP4, produced robust cell death in N. benthamiana. The YFP-labeled SUT1 was localized on the plasma membrane (PM), and Gly2, Cys4, and Ser6 are crucial amino acid sites for its PM localization and function. Further dissection proclaimed that the function and localization of SUT1 are influenced by mutations in conserved specific residues. These findings may provide a new perspective for elucidating the activation mechanism of SUT1. Full article

Background/Objectives: The pattern of invasion describes the arrangement of neoplastic cells along the tumor infiltrative front and refers to the way cancer infiltrates tissue at the tumor/host interface. Accumulating evidence suggested that the Worst Pattern of Invasion (WPOI) represents an independent prognostic factor in oral squamous cell carcinoma (OSCC). However, it is still considered a minor prognostic criterion, and it is recommended as an optional report component in the College of American Pathologists (CAP) guideline. Methods: Therefore, the study aims to extensively review the literature data regarding the prognostic role of the WPOI in OSCC. Results: The WPOI resulted as an independent prognostic factor for locoregional recurrences (LRRs), lymph node metastasis (LMN), overall survival (OS), disease-specific survival (DSS), and bone tissue infiltration, regardless of the oral subsite and the pathological stage. Moreover, several authors suggested the evaluation of the WPOI to lead the postoperative management and to determine the occult LNM in early-stage OSCC. Conclusions: The prognostic relevance of the WPOI in OSCC highlights its evaluation in pathological daily practice. Therefore, the WPOI-detection method and scoring system should be validated based on the tumor stage and site. Full article

Coastal settlements worldwide face increasing threats from erosion, and the Monrovia coastline in Liberia is no exception. This study investigates shoreline dynamics along a 20.5 km stretch of Monrovia’s coast, which is characterized by low-lying elevations, gentle slopes, and sandy beaches. Using Landsat satellite imagery (1986–2025), supported by Sentinel-2 MSI and qualitative validation drone data, we analyzed historical shoreline change with remote sensing and GIS techniques. Shorelines were extracted using a band-ratio thresholding method and quantified with the Digital Shoreline Analysis System (DSAS 5.0), applying end-point rate (EPR), linear regression rate (LRR), and net shoreline movement (NSM). Exploratory projections for 2036 and 2046 were generated using a Kalman Filter model integrated into DSAS. Results show maximum historical erosion rates of up to 3.8 m/yr and accretion rates of up to 5.9 m/yr, with shoreline retreat reaching 150 m and advance up to 194 m. Erosion hotspots are projected for Hotel Africa, Westpoint, New Kru Town, and the JFK–ELWA corridor, while areas near the St. Paul and Mesurado estuaries are expected to accrete. These findings confirm historical trends and suggest that Monrovia will continue to face significant shoreline change, with implications for natural habitats, infrastructure, land loss, and population displacement. Full article

Background: Complete rectal prolapse (RP) is a debilitating pelvic floor disorder often accompanied by obstructed defecation syndrome (ODS), fecal incontinence, and LARS-like bowel dysfunction. Laparoscopic resection rectopexy (LRR) is an established abdominal approach; however, functional outcomes after LRR with transanal specimen extraction (LRR-TSE) are incompletely defined. Aim: To evaluate short- and long-term functional outcomes—ODS, Wexner incontinence score (WIS), and LARS—in patients undergoing LRR-TSE. Methods: This single-center cohort included 53 consecutive patients who underwent LRR-TSE between January 2013 and December 2019. Variables were prospectively recorded and analyzed retrospectively. ODS, WIS, and LARS scores were assessed preoperatively and at 3, 6, and 12 months. Longitudinal changes were analyzed using repeated-measures ANOVA with Greenhouse–Geisser correction, polynomial contrasts when appropriate, and Bonferroni-adjusted pairwise comparisons. Results: ODS improved significantly over time (p < 0.001), decreasing from 12.8 ± 3.2 preoperatively to 2.4 ± 2.1, 4.2 ± 2.2, and 5.2 ± 2.9 at 3, 6, and 12 months, respectively. LARS scores declined from 18.0 ± 12.7 at 3 months to 8.8 ± 6.8 at 6 months and 3.5 ± 4.2 at 12 months (p < 0.001). WIS showed a transient increase at 3 months (8.1 ± 5.2), followed by improvement at 6 and 12 months (3.2 ± 3.7 and 2.4 ± 3.0; p < 0.001). Sex and body mass index did not affect functional trajectories (p > 0.05), whereas patients aged ≥50 years had higher postoperative LARS and WIS scores (p < 0.05). Complications occurred in 5 patients (9.43%), including one anastomotic leak with a mortality rate of 1.85%. Full-thickness recurrence occurred in 2 patients (3.77%), and 3 developed mucosal prolapse managed with Delorme’s procedure. Conclusions: LRR-TSE is a safe and feasible minimally invasive technique that improves constipation, continence, and LARS-related bowel dysfunction. Early postoperative impairment may overestimate long-term functional severity, highlighting the need for follow-up beyond 12 months. Full article

Berberine (BBR), a protoberberine alkaloid with a long history of medicinal use, has consistently demonstrated benefits in glucose–lipid metabolism and inflammatory balance across both preclinical and human studies. These diverse effects are not mediated by a single molecular target but by BBR’s capacity to restore network coordination among metabolic, immune, and microbial systems. At the core of this regulation is an AMP-activated Protein Kinase (AMPK)-centered mechanistic hub, integrating signals from insulin and nutrient sensing, Sirtuin 1/3 (SIRT1/3)-mediated mitochondrial adaptation, and inflammatory pathways such as nuclear Factor Kappa-light-chain-enhancer of Activated B cells (NF-κB) and NOD-, LRR- and Pyrin Domain-containing Protein 3 (NLRP3). This hub is dynamically regulated by system-level inputs from the gut, mitochondria, and epigenome, which in turn strengthen intestinal barrier function, reshape microbial and bile-acid metabolites, improve redox balance, and potentially reverse the epigenetic imprint of metabolic stress. These interactions propagate through multi-organ axes, linking the gut, liver, adipose, and vascular systems, thus aligning local metabolic adjustments with systemic homeostasis. Within this framework, BBR functions as a negentropic modulator, reducing metabolic entropy by fostering a coordinated balance among these interconnected systems, thereby restoring physiological order. Combination strategies, such as pairing BBR with metformin, Sodium-Glucose Cotransporter 2 (SGLT2) inhibitors, and agents targeting the microbiome or inflammation, have shown enhanced efficacy and substantial translational potential. Berberine ursodeoxycholate (HTD1801), an ionic-salt derivative of BBR currently in Phase III trials and directly compared with dapagliflozin, exemplifies the therapeutic promise of such approaches. Within the hub–axis paradigm, BBR emerges as a systems-level modulator that recouples energy, immune, and microbial circuits to drive multi-organ remodeling. Full article

On-orbit cutting is a critical process for the on-orbit manufacturing of carbon fiber reinforced polyetheretherketone composites (CF/PEEK) truss structures, with pulsed laser cutting serving as one of the feasible methods. Achieving high-quality cutting of CF/PEEK remains a major challenge for on-orbit manufacturing. Therefore, the cutting process of CF/PEEK prepreg tape was studied by an ultraviolet (UV) nanosecond laser. A three-factor, five-level orthogonal experiment was carried out to analyze the influence of laser repetition rate (LRR), laser cutting speed (LCS), and laser scanning times (LCTs) on cutting quality. The ablation mechanism dominated by the photothermal effect between the UV nanosecond laser and CF/PEEK was analyzed, and the by-products in the cutting process were explored. Finally, the optimal cutting quality (the width of slit (W_s) = 41.69 ± 3.54 μm, the heat-affected zone (HAZ) = 87.27 ± 7.30 μm) was obtained under the process conditions of LRR 50 kHz-LCS 50 mm/s-LCT 16 times. The findings show that the W_S and HAZ increase with the increase in LRR and LCT and the decrease in LCS, and the carbon fiber decomposes and escapes due to the photothermal effect. Full article

Atherosclerosis is a chronic, multifactorial vascular disease and the leading global cause of cardiovascular morbidity. Its development reflects interconnected disturbances in lipid metabolism, endothelial function, inflammation, smooth muscle cell (SMC) phenotypic switching, and extracellular matrix remodeling. Genetic predisposition, including monogenic disorders such as familial hypercholesterolemia and polygenic risk variants, modulates disease susceptibility by altering lipid homeostasis as well as inflammatory and thrombotic pathways. Epigenetic regulators and noncoding RNAs, such as histone modifications, microRNAs, and long noncoding RNAs, further shape gene expression and link environmental cues to vascular pathology. Endothelial injury promotes lipoprotein retention and oxidation, triggering monocyte recruitment and macrophage-driven foam cell formation, cytokine secretion, and necrotic core development. Persistent inflammation, macrophage heterogeneity, and SMC plasticity collectively drive plaque growth and destabilization. Emerging insights into immune cell metabolism, intracellular signaling networks, and novel regulatory RNAs are expanding therapeutic possibilities beyond lipid-lowering. Current and evolving treatments include statins, proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors, anti-inflammatory agents targeting interleukin-1 beta (IL-1β) or NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3), and advanced approaches such as gene editing, siRNA, and nanoparticle-based delivery. Integrating multi-omics, biomarker-guided therapy, and precision medicine promises improved risk stratification and next-generation targeted interventions. This review summarizes recent molecular advances and highlights translational opportunities for enhancing atherosclerosis prevention and treatment. Full article

Municipal animal shelters play a crucial role in rescuing abandoned animals, managing free-roaming populations, and promoting animal welfare. This seven-year retrospective study analyzed 2291 animals (1132 dogs, 1159 cats) admitted to a Portuguese municipal shelter (CVM Feira) between 2018 and 2024 to identify predictors of live outcomes. Descriptive and regression analyses were performed on 1325 adoptable animals. Adoption occurred for 58.9% of these animals, while 41.1% remained in long-term housing, reflecting growing shelter capacity pressures. Length of Stay (LOS) was the strongest predictor of adoption, with extended stays markedly reducing adoption odds and contributing to overcrowding. For dogs, female sex and owner or animal protection association (APA) surrender increased adoption likelihood. Microchipped animals were 71 times more likely to be returned to their owners, although only a small proportion entered the shelter with identification. The overall Live Release Rate (LRR) was 79.7% and Save Rate (SR) 84.7%, highlighting effective life-saving efforts, while the Population Balance Calculation (PBC) of 75.6% revealed challenges in population flow-through. These findings provide evidence that data-driven strategies, including reducing LOS, enforcing microchipping, and applying species-specific management, are essential to optimize municipal shelter operations and improve animal welfare, with insights applicable to broader European and international contexts. Full article

Background/Objectives: Breast cancer is the most common cancer among women worldwide. Surgical treatments include breast-conserving therapy (BCT) and mastectomy, often followed by reconstruction, but the impact of reconstruction on locoregional recurrence (LRR) remains unclear. This study evaluated LRR, survival, and risk factors following primary breast reconstruction performed simultaneously with mastectomy compared with mastectomy without reconstruction. Methods: This population-based, retrospective cohort included 2475 women with breast cancer treated between 2004 and 2018 at the Tumor Center and Caritas St. Josef Hospital in Regensburg, Germany. Patients were grouped into not primarily reconstructed, primary autologous reconstruction, primary allogeneic reconstruction, and primary combined reconstruction. Overall survival (OS), recurrence-free survival (RFS), and cumulative recurrence rates (CRR) were assessed using Kaplan–Meier methods and Cox proportional hazards models adjusted for age, nodal status, tumor biology, and adjuvant therapies. Results: Of 14,046 eligible cases, 2475 met inclusion criteria: no primary reconstruction (87%), autologous reconstruction (3.1%), allogeneic reconstruction (9.0%), and combined reconstruction (0.4%). Patients undergoing reconstruction were younger and more likely to receive chemotherapy. The 5-year OS was 71.8% without primary reconstruction, 82.1% after autologous reconstruction, and 90.0% after allogeneic reconstruction. Allogeneic reconstruction was associated with improved OS (HR 0.570, p = 0.015) and RFS (HR 0.669, p = 0.039), whereas autologous reconstruction was associated with higher hazards of LRR and distant metastases compared to no primary reconstruction. Conclusions: The 5-year cumulative LRR was 5.2%, 13.5%, and 4.8%, respectively. Immediate allogeneic reconstruction after mastectomy was therefore associated with favorable survival and recurrence outcomes, while autologous reconstruction was linked to higher LRR and distant metastasis rates in this cohort. The retrospective design, small autologous subgroup, and absence of detailed lifestyle and metabolic data are important limitations of these findings. These associations likely reflect differences in tumor stage, biology, and unmeasured risk factors, and should be interpreted as hypothesis generating. Prospective multicenter studies with detailed risk profiling are needed to clarify the oncologic safety of different reconstructive strategies. Full article

Perennial ryegrass is a widely cultivated cool-season forage and turf grass species whose growth and development are limited by drought and high temperature. MAX2 is an F-box leucine-rich repeat (LRR) protein, which serves as a central component of strigolactone (SL) and karrikin (KAR) signaling pathways, involved in multiple growth and developmental processes as well as stress response. Here, we identified LpMAX2, a perennial ryegrass (Lolium perenne L.) homolog of Arabidopsis MAX2 (AtMAX2) and rice D3. LpMAX2 can interact with AtD14 and LpD14 in an SL-dependent manner, implying functional conservation with AtMAX2. Overexpression of LpMAX2 in the Arabidopsis max2-3 mutant partially rescued leaf morphology, hypocotyl elongation, and branching phenotypes, while fully restoring drought tolerance, highlighting the evolutionarily conserved roles of MAX2 in plant growth and drought resistance. In conclusion, LpMAX2 is evolutionarily conserved in SL/KAR signaling pathways, highlighting its potential function in drought adaptation. In addition to elucidating the biological function of LpMAX2, this study identifies a promising genetic target for enhancing stress resilience in forage grasses through biotechnological approaches. Full article