Search Results (69)

The increasing global demand for energy and the urgent need to mitigate climate change have driven the search for sustainable alternatives to fossil fuels, with biofuels emerging as a promising solution. However, the low yields and inefficiencies in biofuel production processes necessitate advanced strategies to enhance their commercial viability. Metabolic engineering has become a pivotal tool in optimizing microbial pathways to improve biofuel production, addressing these challenges through innovative genetic and synthetic biology approaches. This review highlights the role of metabolic engineering in enhancing biofuel production by focusing on microbial engineering for lignocellulosic biomass utilization, strategies to overcome inhibitor effects, and pathway optimization for biofuels like n-butanol and iso-butanol. It also explores the production of advanced biofuels from fatty acid and isoprenoid pathways, emphasizing the use of model organisms such as Escherichia coli and Saccharomyces cerevisiae. Key insights include the application of CRISPR/Cas9 and multiplex automated genome engineering for precise genetic modifications, as well as metabolic flux analysis to optimize pathway efficiency. Additionally, the review discusses synthetic biology methodologies to rewire metabolic networks and improve biofuel yields, providing a comprehensive overview of current advancements and their implications for industrial-scale production. Full article

Composite materials are increasingly used in aerospace applications due to their high strength-to-weight ratio, but their fire safety remains a critical concern. This study develops a coupled thermochemical model to predict the thermal response and decomposition behavior of composite materials under high-temperature fire conditions. The framework integrates heat transfer, resin pyrolysis kinetics, and gas generation dynamics, employing the Rule of Mixtures to dynamically update temperature-dependent thermophysical properties (thermal conductivity, specific heat capacity, and density). Decomposition kinetics are governed by an n-th-order Arrhenius equation, explicitly resolving the gas convection effects on energy transport. The governing equations are solved numerically using a hybrid explicit/implicit finite element scheme, ensuring stability under severe thermal gradients. Experimental validation compliant with the 14 CFR Part 25 and ISO 2685 standards demonstrates high predictive accuracy. The model successfully captures key phenomena, including the char layer insulation effects, transient heat flux attenuation, and decomposition-induced property transition. This work establishes a computational foundation for optimizing fire-resistant composites in aerospace applications, addressing critical gaps in the existing models through coupled multiphysics representation. Full article

Heat stress on workers wearing PPE (Personal protective equipment) in hot outdoor environments is of rising concern, especially in cases when rest breaks and clothing changes are impractical. Mist fan evaporative cooling could provide low-energy continuous cooling, even during work activity. The cooling effect of a misting fan was compared to that of a fan alone, as well as natural convection. A thermal mannequin with heat flux sensors at eight body locations was exposed to an outdoor misting fan while being clothed in typical work clothes and PPE. Work clothes were dry or saturated with water to simulate sweat. The distance from the misting fan ranged from 4 m (wetting common) to 7 m (wetting unlikely). On average, the misting fan had a cooling effect of 0.31 met (18.3 W/m²) higher than natural convection when PPE is worn with wet work clothes, and 0.35 met (20.3 W/m²) higher than when PPE is worn with dry work clothes. This equates to reducing the thermal metabolic load from light industrial work to walking about in office work, or from standing to reclining. Under the ISO 7243 international standard for workers in hot environments, this would increase the acceptable WBGT (wet bulb globe temperature) by over 0.6 °C. Full article

This paper deals with the mitigation of magnetic field levels produced by a wireless power transfer (WPT) system to recharge the battery of an electric vehicle (EV). In this work, an array of active coils surrounding the WPT coils is proposed as a mitigation technique. The theory and new methodological aspects are the focus of the paper. Magnetic field levels in the environment are calculated numerically without and with the presence of an array of active coils in a stationary WPT system for automotive applications. By the proposed mitigation method, the field levels beside the vehicle are significantly reduced and comply with the reference levels (RLs) of the ICNIRP 2010 guidelines for human exposure to electromagnetic fields and the magnetic flux density limits proposed by ISO 14117 for electromagnetic interference (EMI) in cardiac implantable electronic devices (CIEDs). Full article

The aim of this study is to predict the distribution of temperature at various positions on silver-coated firefighter protective clothing when subjected to external radiant heat flux. This will be helpful in the determination of thermal protective performance. Firefighter clothing consists of three layers, i.e., the outer shell, moisture barrier and thermal liner. The outer shell is the exposed surface, which was coated with silver particles through a physical vapor deposition process called magnetron sputtering. Afterwards, these uncoated and silver-coated samples were exposed to radiant heat transmission equipment at 10 kW/m² as per the ISO 6942 standard. Silver-coated samples displayed better thermal protective performance as the rate of temperature rise in silver-coated samples slowed. Later, a numerical approach was employed, contemplating the impact of metallic coating on the exterior shell. The finite difference method was utilized for solving partial differential equations and the implicit method was employed to discretize the partial differential equations. The numerical model displayed a good prediction of the distribution of temperature at different nodes with respect to time. The comparison of time vs. temperature graphs at different nodes for uncoated and silver-coated samples acquired from numerical solutions showed similar patterns, as witnessed in the experimental results. Full article

The accurate evaluation of the thermal performance of building envelope components (e.g., facade walls) is crucial for the reliable evaluation of their energy efficiency. There are several methods available to quantify their thermal resistance, such as analytical formulations (e.g., ISO 6946 simplified calculation method), numerical simulations (e.g., using finite element method), experimental measurements under lab-controlled conditions or in situ. Regarding measurements, when using the heat flow meter (HFM) method, very often, the measured value is based on surface conditions (e.g., temperature and heat flux), achieving in this way the so-called surface-to-surface or conductive thermal resistance ($R_{cond}$). When the building components are made of homogeneous layers, their $R_{cond}$ values are constant, regardless of their internal and external surface boundary conditions. However, whenever this element is composed of inhomogeneous layers, such as in lightweight steel-framed (LSF) walls, their $R_{cond}$ values are no longer constant, depending on their thermal surface resistance. In the literature, such systematic research into how these $R_{cond}$ values vary is not available. In this study, the values of four LSF walls were computed, with different levels of thermal conductivity inhomogeneity, making use of four finite elements’ numerical simulation tools. Six external thermal surface resistances ($R_{\mathrm{s}\mathrm{e}}$) were modelled, ranging from 0.00 up to 0.20 m²·K/W. The average temperature of the partition LSF walls is 15 °C, while for the facade LSF walls it is 10 °C. It was found that the accuracy values of all evaluated numerical software are very high and similar, the $R_{cond}$ values being nearly constant for walls with homogeneous layers, as expected. However, the variation in the $R_{cond}$ value depends on the level of inhomogeneity in the LSF wall layers, increasing up to 8%, i.e., +0.123 m²·K/W, for the evaluated $R_{\mathrm{s}\mathrm{e}}$ values. Full article

Currently, there are no reliable prognostic factors to determine which upper tract urothelial carcinoma (UTUC) patients will progress after radical nephroureterectomy (RNU). We aim to evaluate whether liquid-biopsy-based biomarkers (circulating tumor cells (CTCs), cell-free DNA (cfDNA), and circulating tumor DNA (ctDNA)) were able to predict clinical outcomes in localized UTUC patients undergoing RNU. Twenty patients were prospectively enrolled between 2021 and 2023. Two blood samples were collected before RNU and three months later. CTCs and cfDNA were isolated and evaluated using the IsoFlux system and Quant-iT PicoGreen dsDNA kit, respectively. Droplet digital PCR was performed to determine ctDNA status. Cox regression analysis was performed on CTCs, cfDNA, and ctDNA at two different follow-up time points to examine their influence on tumor progression and cancer-specific survival (CSS). During a median follow-up of 18 months, seven (35%) patients progressed and three (15%) died. Multivariate analysis demonstrated that cfDNA levels three months after RNU are a significant predictor of tumor progression (HR = 1.085; p = 0.006) and CSS (HR = 1.168; p = 0.029). No associations were found between CTC enumeration and ctDNA status with any of the clinical outcomes evaluated. The evaluation of cfDNA levels in clinical practice could improve the disease management of UTUC patients. Full article

Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia, yet the cellular and molecular mechanisms underlying the AF substrate remain unclear. Isolevuglandins (IsoLGs) are highly reactive lipid dicarbonyl products that mediate oxidative stress-related injury. In murine hypertension, the lipid dicarbonyl scavenger 2-hydroxybenzylamine (2-HOBA) reduced IsoLGs and AF susceptibility. We hypothesized that IsoLGs mediate detrimental pathophysiologic effects in atrial cardiomyocytes that promote the AF substrate. Using Seahorse XFp extracellular flux analysis and a luminescence assay, IsoLG exposure suppressed intracellular ATP production in atrial HL-1 cardiomyocytes. IsoLGs caused mitochondrial dysfunction, with reduced mitochondrial membrane potential, increased mitochondrial reactive oxygen species (ROS) with protein carbonylation, and mitochondrial DNA damage. Moreover, they generated cytosolic preamyloid oligomers previously shown to cause similar detrimental effects in atrial cells. In mouse atrial and HL-1 cells, patch clamp experiments demonstrated that IsoLGs rapidly altered action potentials (AP), implying a direct effect independent of oligomer formation by reducing the maximum Phase 0 upstroke slope and shortening AP duration due to ionic current modifications. IsoLG-mediated mitochondrial and electrophysiologic abnormalities were blunted or totally prevented by 2-HOBA. These findings identify IsoLGs as novel mediators of oxidative stress-dependent atrial pathophysiology and support the investigation of dicarbonyl scavengers as a novel therapeutic approach to prevent AF. Full article

This article concerns research on the use of two types of coatings (parylene C and TiO₂-ZrO₂-Al) in multilayer composites with potential use in metallurgical protective gloves to improve their insulation against contact heat and radiation heat. To evaluate the thermal safety of the glove user, the composites were examined under the conditions of exposure to contact heat (using a heating cylinder, according to EN ISO 12127-1) and radiant heat (using a copper plate calorimeter, according to EN ISO 6942). Moreover, heat transfer through composites exposed to the heat of a hot plate was examined using thermography. The experimental studies were supported by heat transfer simulations through 3D models of composites. The contact heat method showed that composites achieved insulation against contact heat for both contact temperatures T_c, but composites with parylene C have a longer t_t of 9 s (for T_c = 100 °C) and 7 s (250 °C) compared to composites with TiO₂-ZrO₂-Al. The radiant heat method showed that composites achieved the fourth (highest) level of RHTI₂₄ under exposure to a radiant heat flux of 20 kW m⁻². The modeling results showed that the parylene C coating increases the thermal barrier of the composite by approximately 10%, while the TiO₂-ZrO₂-Al coating increases it by 2%. The applied research techniques demonstrated the usefulness of using both types of coatings in the design of metallurgical protective gloves based on multilayer composites. Full article

As part of a contract with ENRESA (National Radioactive Waste Company S.A. is a Spanish public company responsible for the management of radioactive waste), after the closure of the uranium mill factory in Andújar, Spain, continuous measurements of the radon flux have been carried out on an annual basis using activated carbon detectors following a methodology established in our laboratory (ISO 11665-7, 2012). The results obtained and their usefulness are presented from the point of view of control of the closure conditions established by the competent authority in order to minimize the impact of the site on the environment. Full article

Macrophage polarization is highly involved in autoimmunity. M1 polarized macrophages drive inflammation and undergo metabolic reprogramming, involving downregulation of mitochondrial energy production and acceleration of glycolysis. Macrophage migration inhibitory factor (MIF), an enigmatic tautomerase (ketonase and enolase), was discovered to regulate M1 polarization. Here, we reveal that KRP-6, a potent and highly selective MIF ketonase inhibitor, reduces MIF-induced human blood eosinophil and neutrophil migration similarly to ISO-1, the most investigated tautomerase inhibitor. We equally discovered that KRP-6 prevents M1 macrophage polarization and reduces ROS production in IFN-γ-treated cells. During metabolic reprogramming, KRP-6 improved mitochondrial bioenergetics by ameliorating basal respiration, ATP production, coupling efficiency and maximal respiration in LPS+IFN-γ-treated cells. KRP-6 also reduced glycolytic flux in M1 macrophages. Moreover, the selective MIF ketonase inhibitor attenuated LPS+IFN-γ-induced downregulation of PARP-1 and PARP-2 mRNA expression. We conclude that KRP-6 represents a promising novel therapeutic compound for autoimmune diseases, which strongly involves M1 macrophage polarization. Full article

In this paper, we propose an innovative spaceborne isoflux scanning digital phased array (ISDPA) design with two-stage digital beamforming (DBF) for geostationary satellites. To achieve isoflux scanning, a novel technique is presented to obtain an isoflux beam for the ISDPA equivalent element using a DBF completely shared subarray architecture and the differential evolution (DE) algorithm. By reutilizing the radiating elements of adjacent subarrays, the radiation aperture and element number are augmented, enhancing the degrees of optimization freedom. To validate the proposed design, a linear ISDPA with 16 DBF completely shared subarrays is optimized and analyzed using two sets of excitation coefficients in different DBF stages. The numerical results demonstrate that the proposed ISDPA can adaptively compensate for space loss variations during beam scanning for geostationary communications with low sidelobes better than −20 dB. Full article

This work presents research concerning the numerical assessment of two previously measured temperatures due to firebrand accumulation on surfaces, which was determined in former thermal experimental campaigns. A 3D numerical model using thermal transient non-linear analysis is used to validate the thermal outputs of these two previous experimental campaigns, and therefore, corroborating the previous temperature vs. time curves created with a prescribed flux in the firebrand accumulation area. The firebrand thermal heat transfer to the plane surface is simulated using convection and radiation film conditions, in which a 3D non-linear, time-dependent finite element simulation is used. Then, the previous proposed standard firebrand accumulation curve, ISO 834, and external fire curve are numerically compared with the results from previous firebrand accumulation curves in a wood corner wall. Finally, the merit assessment of the proposed standard firebrand accumulation curve shows a visible improvement, which has low values and is in accordance with the experimental results in the temperature field distribution of firebrand accumulation onto a contact surface. It is fair to argue that it constitutes a point to search for an efficient design for structures at elevated temperatures due to firebrand accumulation. Full article

Locally advanced rectal cancer (LARC) has traditionally been treated with trimodality therapy consisting of neoadjuvant radiation +/− chemotherapy, surgery, and adjuvant chemotherapy. There is currently a clinical need for biomarkers to predict treatment response and outcomes, especially during neoadjuvant therapy. Liquid biopsies in the form of circulating tumour cells (CTCs) and circulating nucleic acids in particular microRNAs (miRNA) are novel, the latter also being highly stable and clinically relevant regulators of disease. We studied a prospective cohort of 52 patients with LARC, and obtained samples at baseline, during treatment, and post-treatment. We enumerated CTCs during chemoradiation at these three time-points, using the Isoflux^TM (Fluxion Biosciences Inc., Alameda, CA, USA) CTC Isolation and detection platform. We then subjected the isolated CTCs to miRNA expression analyses, using a panel of 106 miRNA candidates. We identified CTCs in 73% of patients at baseline; numbers fell and miRNA expression profiles also changed during treatment. Between baseline and during treatment (week 3) time-points, three microRNAs (hsa-miR-95, hsa-miR-10a, and hsa-miR-16-1*) were highly differentially expressed. Importantly, hsa-miR-19b-3p and hsa-miR-483-5p were found to correlate with good response to treatment. The latter (hsa-miR-483-5p) was also found to be differentially expressed between good responders and poor responders. These miRNAs represent potential predictive biomarkers, and thus a potential miRNA-based treatment strategy. In this study, we demonstrate that CTCs are present and can be isolated in the non-metastatic early-stage cancer setting, and their associated miRNA profiles can potentially be utilized to predict treatment response. Full article

This study will present a comprehensive review of the two-phase flow boiling heat transfer coefficient of hydrocarbons such as propane (R-290), butane (R-600), iso-butane (R-600a), and ethanol at various experimental conditions. Studying the multiphase flow heat transfer coefficient is crucial for many types of heat transfer equipment to achieve higher efficiency for more compact design and cost reduction. One reason we chose hydrocarbons as refrigerants in this study is that they are of an ozone depletion potential equal to zero (ODP = 0) and a deficient level of direct global warming potential (GWP = 3). Moreover, hydrocarbons’ thermodynamic and thermophysical characteristics qualify them to be a strong candidate for more heat transfer applications, initially, by constructing a database for the working fluids using multiple existing experimental work. The current data that this study have collected for the flow boiling spans a wide range of parameters, such as mass flux, heat flux, operating pressure, saturation temperature, etc. Furthermore, by comparing the experimental multiphase heat transfer coefficient database with the anticipated values of each correlation, the prediction performance of 26 correlations found in the literature was assessed. This study allows the best prediction method to be selected based on the minimum deviation of predicted results from the experimental database provided based on the mean absolute error (MAE) calculated from the assessed correlations. The conclusions of such a study can also be helpful for developing more accurate correlation methods for these fluids and improving the prediction of their flow boiling characteristics. Full article