Search Results (143)

Power hardware-in-the-loop (PHIL) creates a safe test environment to connect simulations with real hardware under test (HuT). Therefore, an interface algorithm (IA) must be chosen. The ideal transformer method (ITM) and the partial circuit duplication (PCD) are popular IAs, where a distinction is made between voltage- (V-) and current-type (C-) IAs. Depending on the sample time of the simulator and further delays, simulation accuracy is reduced and instability can occur due to negative feedback in the V-ITM and C-ITM control loops, which makes PHIL operation impossible. In the case of positive feedback, such as with the V-PCD and C-PCD, the delay causes destructive interference, which results in a phase shift and attenuation of the output signal. In this article, a novel damped Smith predictor (SP) for positive feedback PHIL IAs is presented, which significantly reduces destructive interference while allowing stable operation at low linking impedances at V-PCD and high linking impedances at C-PCD, thus reducing losses in the system. Experimental results show a reduction in phase shift by 21.17° and attenuation improvement of 24.3% for V-PCD at a sample time of 100 µs. The SP transfer functions are also derived and integrated into the listed negative feedback IAs, resulting in an increase in the gain margin (GM) from approximately one to three, which significantly enhances system stability. The proposed methods can improve stability and accuracy, which can be further improved by calculating the HuT impedance in real-time and dynamically adapting the SP model. Stable PHIL operation with SP is also possible with SP model errors or sudden HuT impedance changes, as long as deviations stay within the presented limits. Full article

Background/Objectives: The influence of single-nucleotide polymorphisms (SNPs) on hepatocellular carcinoma (HCC) in terms of etiological factors remains to be explored. This study evaluated the distribution of PNPLA3 rs738409, TM6SF2 rs58542926, and HSD17B13 rs6834314 and overall survival of HCC patients with metabolic dysfunction-associated steatotic liver disease (MASLD-HCC) and viral-related HCC (VIRAL-HCC). Methods: This study included 564 patients with HCC: 254 with MASLD-HCC and 310 with VIRAL-HCC. The SNPs were determined by real-time PCR using TaqMan assays. Results: The mean ages of patients with MASLD-HCC and VIRAL-HCC were 68.4 vs. 60.9 years (p < 0.001), with a significant difference between groups. The prevalence of PNPLA3 GG genotype in MASLD-HCC was significantly higher in MASLD-HCC than in VIRAL-HCC (24.0% vs. 15.5%, OR = 1.86, 95% CI = 1.14–3.05, p = 0.009). Similarly, the prevalence of TM6SF2 TT genotype in MASLD-HCC and VIRAL-HCC was 7.1% vs. 2.6% (OR = 3.39, 95% CI = 1.36–9.21, p = 0.003), while HSD17B13 GG genotype in the corresponding groups was 7.1% vs. 12.6% (OR = 0.53, 95% CI = 0.27–1.01, p = 0.039). The overall median survival of MASLD-HCC was significantly shorter than that of the VIRAL-HCC group (42 vs. 66 months, p = 0.035). In Cox regression hazard analysis, HSD17B13 GG genotype was significantly associated with a lower mortality rate in MASLD-HCC (HR = 0.38, 95% CI = 0.18–0.81, p = 0.011). In contrast, PNPLA3 and TM6SF2 were not associated with overall survival in patients with MASLD-HCC or VIRAL-HCC. Conclusions: Our data demonstrated that the prevalence of the SNPs significantly differed between MASLD-HCC and VIRAL-HCC. The HSD176B13 GG genotype was also associated with a survival benefit in Thai patients with MASLD-HCC. Thus, assessing the HSD176B13 genotype might be beneficial in risk stratification and potential therapeutic inhibition of HSD17B13 among patients with MASLD-HCC. Full article

Objective: To assess radiographic changes in the alveolar bone on the distal aspect of the second molars (2Ms) over time, while impacted third molars (ITMs) remain present across two timepoints. Methods: This retrospective observational study aimed to assess radiographic changes between two timepoints (T0 and T1). Both Orthopantomogram (OPG) and Periapical (PA) X-rays were utilized, with three measurements taken on the distal surface of 2Ms using EMAGO 6.1 software. Statistical significance was defined as a p-value < 0.05. Results: A total of 51 patients met the inclusion criteria, with a mean age of 45 years (SD ± 13). Sixty-eight second molars were assessed at baseline (T0) and follow-up (T1), with a mean interval of 20 months (SEM ± 62 days). No significant changes were found in vertical, oblique, or angular bone levels between T0 and T1. Gender significantly affected the cementoenamel junction (CEJ)–base of defect (BD) measurements (p = 0.022) and defect angles at T0 (p = 0.048), but not at the adjusted T1 (p = 0.292). Other variables, including medical history, smoking, and ITM angulation, showed no influence. Patient age was borderline significant in relation to intrabony defect angle (p = 0.047). Conclusions: Considering its limitations, this analysis does not provide evidence to support the hypothesis that prophylactic extraction of ITMs yields significant bone-sparing benefits. Furthermore, it does not establish that prolonged retention of ITMs consistently results in short-term bone alterations in adjacent 2Ms. Consequently, further research is warranted to more accurately assess the medium- to long-term implications of ITM retention on the bone levels of 2Ms. Full article

Three isolates of a novel, rapidly growing, non-pigmented Mycobacterium species were recovered from the water and runoff of a public fountain in a rural village in central Portugal, formerly used by the local population as a source of drinking water and not accessible to animals. High-quality draft genome sequencing, in silico DNA–DNA hybridization, and phylogenetic analyses confirmed that isolates 21AC1^T, 21AC14, and 21AC21 represent a previously undescribed species within the genus Mycobacterium, forming a distinct phylogenetic lineage closely related to Mycobacterium wolinskyi, Mycobacterium goodii and Mycobacterium smegmatis. MALDI-TOF MS analysis of the type strain 21AC1^T revealed a unique spectral profile. A comprehensive polyphasic characterization was performed, including chemotaxonomic analyses of fatty acid and mycolic acid composition, as well as an extensive biochemical characterization. Their susceptibility to 12 antimicrobials was also assessed. The identification and characterization of novel nontuberculous mycobacteria species are of increasing environmental and clinical relevance, as infections by these opportunistic pathogens are on the rise globally. Based on our findings, we propose that isolates 21AC1^T, 21AC14, and 21AC21 represent a novel species, for which we propose the name Mycobacterium appelbergii sp. nov., with the type strain designated as 21AC1^T (=BCCM/ITM 501212 = DSM 113570) and the additional two strains as 21AC14 (=BCCM/ITM 501447 = DSM 118402) and 21AC21 (=BCCM/ITM 501448 = DSM 118403). Full article

The study of gene anomalies linked to thyroid cancer is gaining more and more attention, and these molecular indicators can offer scholarly support for thyroid cancer diagnosis, therapy selection, and prognosis. Genotype–tissue expression pan-cancer data and The Cancer Genome Atlas (TCGA) were used to investigate the expression of GZMK, TREM2, and OR4D10. In order to assess the relationship between GZMK, TREM2, and OR4D10 expression and patient outcome, TCGA clinical survival data were used. We used the clusterProfiler R software tool to conduct enrichment analysis of GZMK, TREM2, and OR4D10. Moreover, TCGA database analysis was used to assess the relationship between immune cell infiltration and GZMK, TREM2, and OR4D10 expression. GZMK, TREM2, and OR4D10 were strongly expressed in several kinds of malignancies including thyroid cancer. Gene sets related to proliferation that are involved in leukocyte cell–cell adhesion and mononuclear cell differentiation were significantly correlated with high expression of GZMK, TREM2, and OR4D10. Additional investigation revealed a correlation between high T cell and DC (dendritic cell) infiltration scores and high expression of GZMK, TREM2, and OR4D10. According to our research, OR4D10, TREM2, and GZMK could all be genes associated with thyroid cancer. Full article

Introduction: Bell’s palsy is a common acute peripheral neurological disorder causing unilateral facial paralysis. Its exact etiology remains unknown, but it is linked to inflammation, immune responses, infections, and ischemia. This study explores the potential causal relationship between Bell’s palsy and peripheral blood inflammatory proteins, metabolites, and immune cell characteristics. Methods: Genetic data for Bell’s palsy were obtained from the Finnish database (version R10) and IEU OpenGWAS. A two-sample Mendelian randomization (MR) approach was applied, analyzing 4907 plasma proteins, 731 immune cell traits, 91 inflammatory proteins, and 1400 metabolites. The Finnish dataset served as the discovery cohort, while the IEU OpenGWAS dataset acted as the validation cohort. Bioinformatics analyses included protein–protein interaction (PPI) networks, Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, colocalization, and Linkage Disequilibrium Score Regression (LDSC) to identify candidate proteins and explore potential therapeutic targets. Results: MR analysis identified 70 inflammatory proteins, 77 metabolites, and 26 immune cell traits as potentially causally associated with Bell’s palsy. After external validation, BLVRB, HMOX2, TNFRSF12A, DEFB128, ITM2A, VEGF-A, and DDX58 remained significantly associated (p < 0.05). PPI network analysis led to 31 candidate proteins, and six core proteins (JAK2, IL27RA, OSM, CCL19, SELL, VCAM-1) were identified. Conclusions: Our study identifies causal relationships between inflammatory proteins, metabolites, immune cells, and Bell’s palsy, highlighting that the JAK/STAT signaling pathway may be a potentially critical target for intervention in Bell’s palsy, and that its modulation may provide new directions and opportunities for therapeutic strategies and drug discovery for the disease. Full article

Objective: Intrathecal morphine (ITM) has been administered in recent years to provide postoperative pain control in non-obstetric surgery; however, current research has limited consideration of the recommendations for regular, basic analgesia from clinical guidelines when exploring its efficacy. This systematic review and meta-analysis aimed to compare ITM against alternative methods of analgesia in the presence of multimodal analgesia, for reducing pain scores within the first 24 h postoperatively. Secondary outcomes included postoperative opioid consumption, incidence of opioid-related effects, and time to mobilisation. Methods: Database searches and screening identified 11 trials for inclusion in this review. Pain scores were compared by meta-analysis at 6, 12, and 24 h postoperatively at rest and on movement, with sub-analysis of systemic versus regional techniques. Results: The data found no significant difference between ITM and active comparators for reducing pain scores at rest or on movement at any of the time intervals explored. Sub-analysis demonstrated that regional techniques may provide superior analgesia at 24 h at rest (MD = −1.19; 95% CI [−1.73, −0.66], p < 0.001, I² = 0%) and on movement (MD = 1.27 [0.44, 2.10], p = 0.003, I² = 0%). Cumulative opioid consumption was reduced in ITM groups (MD = −11.61 [−18.73, −4.50], p = 0.001, I² = 95%), with significantly increased risk of pruritus (p < 0.001) but not nausea and vomiting (p = 0.93). There was no evidence of respiratory depression. Conclusions: This meta-analysis was unable to demonstrate any significant benefit to postoperative pain relief with the use of ITM but may suggest that it is as a viable option compared to other active modalities. However, this meta-analysis was limited by a low quantity and quality of data from which to draw conclusions and demonstrated high statistical fragility. We believe this highlights a significant gap in the current literature on ITM. Full article

Background: Changes in the bony structures of the nose and sinuses such as the medialization of the lamina papyracea and enophthalmos have been reported after sinus surgery. Evidence for the persistence of inferior turbinate (IT) position after IT outfracture is lacking. Objectives: To evaluate for anatomical changes of the IT, lamina and the globes, after sinus surgery and the durability of inferior turbinate outfracture. Methods: A total of forty-four patients who underwent revision endoscopic sinus surgery that included complete ethmoidectomy and IT outfracture were matched. Pre- and post-operative computed tomography scans (CT) were used for evaluating and measuring the anatomical changes in different planes. The posterior globe position in the axial plane, the distance between the lamina papyracea (IOD_Axial, IOD_Coronal) in coronal and axial planes and the distance from the IT to the septum (IT_M) and the lateral nasal wall (IT_L) were measured. Results: There were 16 women and 28 men. Mean follow-up time (time from procedure to post-operative CT scan) was 38.9 ± 20.1 months. Statistically significant lateralization of the IT was observed with IT_L (95%CI 1.1 mm to 1.5 mm p < 0.0001) and IT_M (95%CI −1.5 mm to −1.1 mm; p < 0.0001). No statistically significant differences were seen in IOD_Axial and IOD_Coronal in pre-op and post-op CT scans. (p = 0.23 and p = 0.7, respectively) and no significant displacement of the globe in antero-posterior direction was seen (p = 0.915). Conclusions: IT outfracture appears to have a durable effect on IT position that lasts for several years. Ethmoidectomy did not cause the medialization of the laminae nor altered the position of the globes. Full article

In cold environments, traditional independent thermal management systems heavily rely on inefficient Positive Temperature Coefficient (PTC) heaters, which exacerbate range anxiety in vehicles. In this study, an energy management-based control strategy for an integrated thermal management system (ITMS) designed for hybrid electric vehicles (HEVs) is proposed. By coupling the four thermal flow circuits of the entire vehicle and integrating driving modes with heating demands, this strategy achieves full vehicle-level integrated control. Through optimizing the distribution and utilization of heat within the vehicle, this enhances the heating performance of the air source heat pump. The simulation results demonstrate that the proposed strategy significantly reduces the power consumption of the heat pump and improves heating efficiency for both the battery and the cabin. By utilizing waste heat from the motor and the engine, the ITMS increases the heating capacity of the heat pump, particularly in low-temperature environments. Compared to traditional thermal management systems, the ITMS control strategy achieves substantial improvements in both heating time and energy efficiency. Specifically, the system reduces battery heating time by 55.94% and enhances the overall heating performance of the vehicle. Furthermore, the strategy reduces fuel consumption by 5.18%, demonstrating its potential to improve the energy efficiency of HEVs in cold climates. Full article

Temperature is the key monitoring measurement of lithium-ion battery condition monitoring, and it plays a very important role in battery life prediction, thermal runaway warning, and thermal management decision making. Therefore, this paper mainly summarizes the research status of internal temperature monitoring (ITM) method for lithium-ion batteries. Firstly, the lithium-ion battery ITM methods are divided into three types, namely temperature sensor, battery thermal model, and electrochemical impedance spectroscopy (EIS) types. The measurement principle, implementation difficulty, and measurement effect of the above ITM methods are different. The advantages and disadvantages of these methods are analyzed and pointed out. In particular, the five latest ITM methods based on negative temperature coefficient (NTC) temperature sensor, optical fiber sensor, ultrasonic wireless sensor, electrochemical thermal coupling model, and multi-frequency EIS are introduced. Finally, based on the existing research, the future development trend of the above five methods is discussed. Full article

In this paper, we present an original numerical method for the solution of a Blasius problem with extended boundary conditions. To this end, we extend to the proposed problem the non-iterative transformation method, proposed by Töpfer more than a century ago and defined for the numerical solution of the Blasius problem. The proposed method, which makes use of the invariance of two physical parameters with respect to an extended scaling group of point transformations, allows us to solve the Blasius problem numerically with extended boundary conditions by solving a related initial value problem and then rescaling the obtained numerical solution. Therefore, our method is an initial value method. However, in this way, we cannot fix the values of the physical parameters in advance, and if we just need to compute the numerical solution for given values of the two parameters, we have to define an iterative extension of the transformation method. Thus, in this paper, for the problem under study, we define a non-ITM and an ITM based on Lie groups scaling invariance theory. Full article

The article addresses the issue of selecting the initial shape for a drone’s suspended transport container and its impact on the final aerodynamic properties during the flight, specifically aerodynamic drag, based on the simulated pressure differences generated by the suspended container. The motivation arose from the project ITMS2014+:313011 ATR9 titled ‘Research and Development of the Applicability of Autonomous Flying Vehicles in the Fight Against the COVID-19 Pandemic’. The project deals with the transport of biological samples from hospitals to specialized laboratories. These samples, which could be contaminated, necessitated the development of a specialized container meeting all safety criteria. Besides predefined parameters such as the maximum weight of the container, transport speed, dimensions of the internal standardized module for transporting hospital samples, and compliance with required IP (Ingress Protection) and IK (Impact Protection) standards, many other challenges related to the final design solution of the transport container had to be addressed. One such challenge was the fundamental question of what shape the transport container should have, which significantly influences the overall design and construction of the transport module. We attempted to solve this question responsibly and professionally and thoroughly substantiate our results with appropriate simulations in selected simulation software. The outcome was as expected, but it was necessary to adequately justify and support the choice of the shape of the transport container in the context of the project’s resolution. The results are universal and can be applied to other designs for shapes of transport containers in the future or by other researchers working on similar projects. Full article

Integrated thermal management system (ITMS) technology for electric vehicles (EV) has become a major industry research direction. However, R290 refrigerants are still not applied on a large scale in EVs. Therefore, we developed a suitable thermal management system for R290 in this study. This architecture adapts an unusual indirect design, which can coordinate the heat between the air conditioner, battery pack, and electric motor. We focused on heat pump air conditioning systems for EV thermal management; thus, we carried out the performance analysis of R290 under the cooling and heating conditions of our ITMS through an experimental approach. The current study explores various aspects affecting the performance of heat-pump air conditioners: refrigerant charge, electronic expansion valve (EXV) opening, compressor speed, and performance between R290 and R134a under different external temperatures. We aim to improve cooling and heating efficiencies. Among these parameters, the EXV opening and compressor speed have the greatest impact on the performance of the ITMS, as evidenced by the optimal EXV opening and lower compressor speed to maximize the coefficient of performance (COP) and increase the heat transfer rate. In addition, this study has shown that, compared to an ITMS equipped with R134a, R290 has a smaller refrigerant charge, better heat transfer rate and COP under heating conditions, and similar performance under cooling conditions. Full article

This study aimed to develop an aromatic thermosensitive genic male sterile (TGMS) line in indica rice using CRISPR/Cas9 technology. The TMS5 and FGR in the high-quality conventional rice variety Huahang 48 were targeted for editing using CRISPR/Cas9 technology. CRISPR/Cas9 vectors designed for TMS5 and FGR were constructed and introduced into rice calli through Agrobacterium-mediated transformation. Transgenic seedlings were subsequently regenerated, and the target sites of the edited plants were analyzed via sequencing. A total of fifteen T₀ double mutants were successfully obtained. Three mutants without T-DNA insertion were screened in the T₁ generation by the PCR detection of hygromycin gene fragments, and homozygous mutants without T-DNA insertion were screened in the T₂ generation by the sequencing analysis of the mutation sites, named Huahang 48s. Huahang 48s exhibited complete sterility at 24 °C and pollen transfer at 23 °C. The 2-acetyl-1-pyrroline (2-AP) content was detected in the young panicles, leaves, and stems of Huahang 48s. The leaves of Huahang 48s had the highest 2-AP content, contrasting with the absence of 2-AP in HuaHang 48. F₁ hybrids that crossed Huahang 48s with two high-quality restorer lines were superior to the two parents in terms of yield per plant and 1000-grain weight. Huahang 48s has a certain combining ability and application potential in two-line cross breeding. The successful application of CRISPR/Cas9 technology in Huahang 48 established a foundation for developing aromatic TGMS lines, providing both theoretical insights and practical materials for breeding efforts. Full article

This floor pen study explored the effects of feeding mineral methionine hydroxyl analogue chelates (MMHACs) zinc (Zn), copper (Cu), and manganese (Mn) on growth performance, carcass processing weight and quality, nutrient digestibility, gizzard erosion score, and bone parameters of broilers. One-day-old Ross 308 male chicks (n = 384) were randomly allocated to four dietary treatments with eight replicate pens per treatment and 12 birds per pen. The treatments were as follows: (1) inorganic trace mineral ZnSO₄ 110 ppm, CuSO₄ 16 ppm, MnO 120 ppm (ITM); (2) MMHAC Zn 40 ppm, Cu 10 ppm, Mn 40 ppm (M10); (3) Inorganic trace mineral ZnSO₄ 110 ppm, tribasic copper chloride 125 ppm, MnO 120 ppm (T125); (4) MMHAC Zn 40 ppm, Cu 30 ppm, Mn 40 ppm (M30). The birds were fed nutritionally complete wheat sorghum soybean meal-based diets from days 0 to 42 with three feeding phases including starter (days 0–10), grower (days 10–21), and finisher (days 21–42). The findings revealed that birds on the T125, M10, and M30 groups possibly had higher feed intake (p = 0.052) and higher weight gain (p < 0.063) than birds on the ITM group from days 0 to 42. Furthermore, birds fed the M30 diet had higher thigh and drumstick weights compared to those fed the ITM diet at day 42 (p = 0.05). Additionally, birds offered the M30 diet had increased ileal Cu digestibility compared to birds offered the M10 and ITM diets at day 21 (p = 0.006). Gizzard erosion scores and bone parameters were similar between the dietary treatments. Hence, the supplementation of MMHACs to broiler diets at 30 ppm may be a more suitable strategy to increase weight gain and the thigh and drumstick processing weight while maintaining the bone health of broiler chickens. Full article