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15 pages, 1608 KB  
Article
Early Detection and Differentiation of Dragon Fruit Plant Diseases Using Optical Spectral Reflectance
by Priyanka Belbase and Maruthi Sridhar Balaji Bhaskar
Appl. Sci. 2026, 16(7), 3480; https://doi.org/10.3390/app16073480 - 2 Apr 2026
Viewed by 485
Abstract
Dragon fruit (Hylocereus spp.) is an emerging crop in the tropics and subtropics, but its production is increasingly threatened by diseases that reduce yield and profitability. Early diagnosis of these diseases is crucial for timely intervention, yet visual symptoms often appear only [...] Read more.
Dragon fruit (Hylocereus spp.) is an emerging crop in the tropics and subtropics, but its production is increasingly threatened by diseases that reduce yield and profitability. Early diagnosis of these diseases is crucial for timely intervention, yet visual symptoms often appear only after significant infection has occurred. The study aims to evaluate how optical spectral reflectance can detect dragon fruit diseases and identify the most responsive spectral regions. In this study, six major dragon fruit stem diseases: Neoscytalidium stem canker, stem sunburn, anthracnose, Botryosphaeria stem canker, Bipolaris stem rot, and bacterial soft rot were characterized by the goal of identifying unique spectral signatures for early detection and differentiation of each disease. Seventy-two potted dragon fruit plants of three distinct species were grown under four organic vermicompost treatments (0, 5, 10, 20 tons/acre) in both open-field and high-tunnel conditions together, in a randomized complete block design. A handheld spectroradiometer (350–2500 nm) was used to collect reflectance from the diseased and healthy cladodes (stem segment). Various spectral vegetative indices were computed to identify disease-specific features. The results revealed distinct spectral features for each disease. Infected cladodes consistently exhibited higher reflectance especially in the visible region (400–700 nm) and the near-infrared region (900–2500 nm) of the spectrum than healthy cladodes. The Normalized Difference Vegetative Index (NDVI), Green Normalized Difference Vegetative Index (GNDVI), and Spectral Ratio (SR) spectral indices were significantly higher in healthy plants than in diseased ones, reflecting higher chlorophyll concentration and plant biomass. Conversely, the 1110/810 ratio was lower in healthy plants than in diseased plants, suggesting a more compact internal plant structure. Statistical analysis revealed highly significant differences (p < 0.00001) between healthy and diseased spectra in the Red, Green and NIR regions. Linear Discriminant Analysis(LDA) achieved the highest classification accuracy (OA = 0.642, κ = 0.488), though performance was limited for minority classes. These findings demonstrate that targeted spectral sensing can identify dragon fruit diseases before obvious symptoms emerge. By pinpointing disease-specific spectral indices, our study paves the way for early-warning tools such as targeted multispectral sensors or drone-based imaging that would enable growers to intervene sooner and limit losses. These results highlight the potential for development of UAV-based or portable spectral sensors for large-scale, near real-time disease monitoring in dragon fruit production. Full article
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20 pages, 3067 KB  
Article
Evaluation of Sentinel-2 Vegetation Indices for Estimating Leaf Area Index in Cassava Plots
by Kanokporn Promnikorn, Thanpitcha Jenkit, Piya Kittipadakul and Ekaphan Kraichak
AgriEngineering 2026, 8(4), 134; https://doi.org/10.3390/agriengineering8040134 - 1 Apr 2026
Viewed by 557
Abstract
Leaf Area Index (LAI) is critical for monitoring cassava growth and yield prediction, yet ground measurements are time-consuming and labor-intensive for large-scale applications. While satellite-based vegetation indices (VIs) offer a scalable alternative, their performance for cassava LAI estimation remains poorly documented, and optimal [...] Read more.
Leaf Area Index (LAI) is critical for monitoring cassava growth and yield prediction, yet ground measurements are time-consuming and labor-intensive for large-scale applications. While satellite-based vegetation indices (VIs) offer a scalable alternative, their performance for cassava LAI estimation remains poorly documented, and optimal index selection for different growth stages is unclear. This study evaluated the predictive performance of 13 Sentinel-2-derived VIs for estimating ground-measured LAI across cassava growth stages. Ground-LAI was measured monthly using a SunScan Canopy Analyzer from January to June 2022 (2–7 months after planting; MAP) in 47 cassava plots in Nakhon Ratchasima Province, Thailand. Linear mixed-effects models and stage-specific regressions assessed VI predictive performance using Coefficient of determination (R2) and Root Mean Squared Error (RMSE). The Green Normalized Difference Vegetation Index (GNDVI) and Normalized Difference Water Index (NDWI) demonstrated superior performance across all growth stages (R2 = 0.524; RMSE = 0.350), followed by Sentinel-2 LAI Green Index (SeLI R2 = 0.521, RMSE = 0.357). Stage-specific analysis revealed that Ratio Vegetation Index performed best during early growth (2 MAP, R2 = 0.671; RMSE = 0.164) while GNDVI and NDWI excelled during mid-growth (3–5 MAP) and SeLI at late growth (7 MAP, R2 = 0.393; RMSE = 0.422). While the presence of large trees altered the ranking of VI predictive performance, it did not substantially affect estimation errors, suggesting a relatively small impact of spatial heterogeneity on LAI estimation accuracy. These findings identify GNDVI and NDWI as the most operationally suitable Sentinel-2 indices for cassava LAI estimation and demonstrate that stage-specific index selection can improve monitoring accuracy, providing validated tools for regional-scale cassava crop monitoring using freely available satellite data. Full article
(This article belongs to the Section Remote Sensing in Agriculture)
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32 pages, 21931 KB  
Article
Harmonic Phenology Mapping: From Vegetation Indices to Field Delineation
by Filip Papić, Mario Miler, Damir Medak and Luka Rumora
Remote Sens. 2026, 18(7), 1011; https://doi.org/10.3390/rs18071011 - 27 Mar 2026
Viewed by 489
Abstract
Operational agricultural monitoring in the Central European lowlands requires timely parcel boundaries; however, unmarked field edges produce minimal spectral contrast in single-date imagery. Previous works demonstrated that harmonic NDVI encoding enables zero-shot field delineation using foundational models, but the influence of the spectral [...] Read more.
Operational agricultural monitoring in the Central European lowlands requires timely parcel boundaries; however, unmarked field edges produce minimal spectral contrast in single-date imagery. Previous works demonstrated that harmonic NDVI encoding enables zero-shot field delineation using foundational models, but the influence of the spectral index choice on temporal boundaries remained unquantified. This study systematically evaluates eleven vegetation indices—NDVI, GNDVI, NDRE, EVI, EVI2, SAVI, MSAVI, NDWI, CIg, CIre, and NDYVI—within a fixed harmonic phenology encoding pipeline. A one-year PlanetScope time series (15 × 15 km, Slavonija, Croatia) was decomposed via annual sinusoidal regression to extract per-pixel phase, amplitude, and mean parameters. These harmonic descriptors were mapped to HSV colour channels and segmented using the Segment Anything Model without fine-tuning. Official agricultural parcels (PAAFRD, 2025) provided ground truth for pixel-wise, object-wise, and size-stratified evaluation. Performance stratified into three tiers based on object-wise metrics. Soil-adjusted and enhanced-greenness indices (MSAVI, EVI, EVI2, and SAVI) achieved F1 = 0.51–0.52, and mIoU = 0.70–0.71, statistically outperforming standard ratio formulations (NDVI: F1 = 0.49) and chlorophyll indices (CIg, CIre: F1 = 0.45–0.47). Pixel-wise scores remained compressed (F1 > 0.88 across all indices), indicating consistent interior coverage but index-dependent boundary precision. Error analysis revealed scale-dependent patterns: merging dominated small parcels (<10,000 m2), while fragmentation increased with parcel size. Results demonstrate that spectral formulation is a systematic design factor in phenology-based delineation, with soil background correction and dynamic range compression improving seasonal trajectory separability. The harmonic parameters generated by this framework provide feature-ready input for crop classification, suggesting that integrated boundary extraction and crop mapping workflows merit further investigation. Full article
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18 pages, 7157 KB  
Article
High-Throughput Evaluation of Cotton Drought Tolerance Using UAV Multispectral Imagery and XGBoost-Based Machine Learning
by Fuxiang Zhao, Tao Yang, Wei Wang, Wanli Han, Gang Wang, Jinxin Qiao, Xianhui Kong, Li Liu, Aijun Si, Fanlin Wang, Xuwen Wang, Xiyan Yang and Yu Yu
Agronomy 2026, 16(5), 526; https://doi.org/10.3390/agronomy16050526 - 28 Feb 2026
Viewed by 404
Abstract
Drought stress severely constrains cotton yield and fiber quality, but conventional evaluation methods are inefficient and time-consuming. To address this, we developed a high-throughput, non-destructive phenotyping framework by integrating UAV-based multispectral remote sensing with machine learning, using 225 upland cotton (Gossypium hirsutum [...] Read more.
Drought stress severely constrains cotton yield and fiber quality, but conventional evaluation methods are inefficient and time-consuming. To address this, we developed a high-throughput, non-destructive phenotyping framework by integrating UAV-based multispectral remote sensing with machine learning, using 225 upland cotton (Gossypium hirsutum L.) accessions. The accessions were subjected to well-watered (CK) and drought stress (DS) treatments at the flowering and boll-setting stage. Canopy multispectral imagery (Green/Red/Red_edge/Near-infrared bands) was acquired via DJI Mavic 3 Multispectral UAV, and 16 vegetation indices (VIs) were derived. Concurrently, 15 agronomic and fiber quality traits were measured to calculate drought resistance coefficients (DRCs), which were used for principal component analysis (PCA) and comprehensive drought tolerance index (D) construction. Hierarchical clustering categorized the accessions into 6 drought tolerance grades (Groups I–VI). Variable importance analysis identified GNDVI, NGRVI, and NDRE as the most drought-sensitive VIs (% IncMSE > 11). Among four regression models (LR, KNN, LGBM, XGBoost), XGBoost achieved the best performance for D prediction (test set: R2 = 0.785, RMSE = 0.032, MAE = 0.024). This study demonstrates that UAV multispectral data coupled with XGBoost enables accurate, efficient drought tolerance assessment, providing a robust tool for high-throughput germplasm screening and smart agricultural management. Full article
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26 pages, 8605 KB  
Article
The Application of Amino Acids as a Sustainable Strategy for Managing Water Stress in Vineyards
by Fabrício Lopes Macedo, Carla Ragonezi, José Filipe Teixeira Ganança, Humberto Nóbrega, José G. R. de Freitas, Andrés A. Borges, David Jiménez-Arias and Miguel A. A. Pinheiro de Carvalho
Remote Sens. 2026, 18(4), 641; https://doi.org/10.3390/rs18040641 - 19 Feb 2026
Viewed by 450
Abstract
Water scarcity increasingly threatens viticulture in the Macaronesian region due to climatic variability and recurrent droughts. This study evaluated the physiological and productive responses of grapevines (Vitis vinifera L.) to foliar applications of two amino acid-based biostimulants, pyroglutamic acid and pipecolic acid, [...] Read more.
Water scarcity increasingly threatens viticulture in the Macaronesian region due to climatic variability and recurrent droughts. This study evaluated the physiological and productive responses of grapevines (Vitis vinifera L.) to foliar applications of two amino acid-based biostimulants, pyroglutamic acid and pipecolic acid, under contrasting water availability conditions on Madeira Island, Portugal. Three non-irrigated treatments were arranged in a randomized complete block design: T1 (no irrigation and no amino acids), T2 (pyroglutamic acid, without irrigation), and T3 (pipecolic acid, without irrigation), while conventional irrigation (T4) was included as a non-randomized reference. Agronomic parameters and UAV-derived multispectral and thermal data were analyzed during the 2023 (moderate drought) and 2024 (severe drought) growing seasons. Vegetation indices (NDVI, GNDVI, NDRE, NGRDI, and GLI) and the Simplified Crop Water Stress Index (CWSIsi) were used to assess canopy vigor and plant water status. In 2023, T4 showed significantly higher bunch number and total yield, whereas differences among non-irrigated treatments were not statistically significant. Nevertheless, T2 showed consistent numerical trends toward higher yield components and a comparatively more stable canopy thermal response than the untreated control. In 2024, severe drought reduced productivity across all treatments, with no significant difference detected. Yield components were generally strongly correlated, while CWSIsi was negatively associated with vegetation indices, particularly under moderate drought. The NGRDI demonstrated potential as a low-cost RGB-based indicator but requires cautious interpretation. Overall, pyroglutamic acid may represent a complementary strategy to irrigation and UAV-based precision monitoring in drought-prone viticulture, although confirmation through longer-term and higher-powered field studies is required. Full article
(This article belongs to the Special Issue Application of UAV Images in Precision Agriculture)
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24 pages, 6940 KB  
Article
Inversion of SPAD Value in Yellowed Leaves of ‘Kuerle Xiangli’ (Pyrus sinkiangensis Yu) Using Multispectral Images from Drones
by Yuan Dai, Lijun Liu, Shaowen Quan and Xiaoyan Lu
Agriculture 2026, 16(4), 416; https://doi.org/10.3390/agriculture16040416 - 12 Feb 2026
Viewed by 390
Abstract
SPAD values serve as a key physiological indicator for assessing the health status of ‘Kuerle Xiangli’ leaves and for monitoring the occurrence of chlorosis. Rapid, non-destructive acquisition of their spatial distribution provides crucial support for precision orchard management and the scientific correction of [...] Read more.
SPAD values serve as a key physiological indicator for assessing the health status of ‘Kuerle Xiangli’ leaves and for monitoring the occurrence of chlorosis. Rapid, non-destructive acquisition of their spatial distribution provides crucial support for precision orchard management and the scientific correction of leaf yellowing. This study selected six ‘Kuerle Xiangli’ experimental orchards in Tiemenguan City, Bayingolin Mongol Autonomous Prefecture, Xinjiang, as the research area. Using multi-spectral imagery from a DJI Mavic 3 drone and ground-measured SPAD values, four inversion models, RF, XGBoost, SVR, and PLSR, were constructed. Model inputs included vegetation indices (VIs), texture features, and a combination of both. By comparing the accuracy of the different models, the optimal SPAD inversion model for yellowing leaves of ‘Kuerle Xiangli’ was selected and validated in the field. Finally, a spatial distribution map of SPAD values was generated based on the optimal model. The results indicate the following: (1) Feature selection and the fusion of multi-source features significantly enhanced inversion performance. Compared to models using a single feature type, the Random Forest (RF) model that integrated 6 vegetation indices (CIRE, NDRE, LCI, REOSAVI, GNDVI, and NDWI) with 26 texture features performed best. It achieved an R2 = 0.9179, RMSE = 1.9970 and MAE = 1.2284 on the training set, and an R2 = 0.8161, RMSE = 3.4702, and MAE = 2.6799 on the validation set. The model also maintained good performance during field validation in an independent orchard (R2 = 0.7329, RMSE = 1.5823, MAE = 1.3377). (2) The spatial distribution map of SPAD values generated by the optimal model clearly delineates the SPAD ranges and yellowing status across the six orchards. The overall SPAD range across all orchards was 15.7 to 45.7. The order of yellowing severity was LLJ (80.5%) > YHC (68.1%) > LGQ (52.9%) > NKS (46.8%) > LCX (36.4%) > LGL (34.1%). Full article
(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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18 pages, 2295 KB  
Article
Assessment of Vegetation Index Saturation Based on Vertically Stratified Aboveground Biomass in Temperate Meadow Steppe
by Yuli Shi, Yidi Wang, Yiqing Hao, Cong Xu, Fangwen Yang, Zhijie Bai, Dan Zhao, Xiaohua Zhu and Wei Liu
Remote Sens. 2026, 18(4), 554; https://doi.org/10.3390/rs18040554 - 10 Feb 2026
Viewed by 537
Abstract
Grassland aboveground biomass (AGB) is a key indicator of grassland ecosystem structure and function, and its accurate monitoring is of great importance for assessing grassland ecological conditions and supporting sustainable grassland management. Traditional biomass estimation methods based on vegetation indices (VIs) often suffer [...] Read more.
Grassland aboveground biomass (AGB) is a key indicator of grassland ecosystem structure and function, and its accurate monitoring is of great importance for assessing grassland ecological conditions and supporting sustainable grassland management. Traditional biomass estimation methods based on vegetation indices (VIs) often suffer from saturation due to canopy shading. However, comparative studies on VI saturation and the saturation height of AGB detectable by different indices remain limited. In this study, we evaluated 12 commonly used VIs based on field-measured AGB and hyperspectral data in the Hulunbuir meadow steppe. Relationships between vertically accumulated biomass and VIs were analyzed to identify optimal AGB fitting models and to determine the saturation height of each index. Results showed that vertical distribution of AGB followed a unimodal pattern, with biomass peaking at approximately 36 cm in this region. This study employed four models (namely the Linear model, the Logarithmic model, the Power Function model and the Gompertz model) to fit the relationship between the vegetation index and AGB. Among them, Gompertz models consistently outperformed other models, indicating saturation across all indices. Based on saturation height, the 12 VIs were classified into two groups: ARVI, GNDVI, NDRE, OSAVI, and SAVI saturated at 40 cm, whereas DVI, EVI, MSAVI, NDPI, NDVI, RVI, and VARI maintained sensitivity up to 50 cm, demonstrating a stronger anti-saturation capacity. NDVI and NDPI exhibited the highest fitting accuracy and resistance to saturation. These findings validate the saturation limitations of VIs and provide guidance for selecting appropriate indices to improve the accuracy of grassland biomass retrieval. Full article
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20 pages, 2432 KB  
Article
Potential of RGB-Derived Vegetation Indices as an Alternative to NIR-Based Vegetation Indices to Monitor Nitrogen Status in Maize
by Mohammad Mhaidat, Iván González-Pérez, José Ramón Rodríguez-Pérez, Jesús P. Val-Aguasca and Enoc Sanz-Ablanedo
Remote Sens. 2026, 18(3), 528; https://doi.org/10.3390/rs18030528 - 6 Feb 2026
Viewed by 725
Abstract
Unmanned aerial vehicles (UAVs) are increasingly used for crop monitoring, but their widespread adoption is limited since they often rely on non-standard specialized cameras equipped with near-infrared (NIR) sensors. More affordable and scalable crop monitoring solutions would be enabled, however, if data could [...] Read more.
Unmanned aerial vehicles (UAVs) are increasingly used for crop monitoring, but their widespread adoption is limited since they often rely on non-standard specialized cameras equipped with near-infrared (NIR) sensors. More affordable and scalable crop monitoring solutions would be enabled, however, if data could be collected using standard RGB sensors. We compared visible-band indices that incorporate blue spectral range (NDGBI and NDRBI) with traditional NIR-based indices (NDVI and GNDVI) for their effectiveness in monitoring maize growth and nitrogen status. UAV multispectral data capture at different maize growth stages was complemented by ground-based spectroradiometer measurements for calibration and validation. Various agronomic and yield variables (including cornstalk NO3–N content, grain yield, grain moisture, number of corncobs, and grain test weight) were recorded to link spectral responses with plant performance and nutritional status. The results show that the overall performance of the RGB-based approach was comparable to that of the NIR-based approach, with the visible-band indices proving to be highly sensitive to physiological stress, chlorophyll degradation, and nitrogen variability in maize. Our findings highlight the potential of the RGB-based indices to complement or even replace specialized NIR-based indices, providing a cost-effective, high-resolution tool for precision agriculture. Full article
(This article belongs to the Special Issue Perspectives of Remote Sensing for Precision Agriculture)
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32 pages, 29618 KB  
Article
Combining ALS and Satellite Data to Develop High-Resolution Forest Growth Potential Maps for Plantation Stands in Western Canada
by Faezeh Khalifeh Soltanian, Luiz Henrique Terezan, Colin E. Chisholm, Pamela Dykstra, William H. MacKenzie and Che Elkin
Remote Sens. 2026, 18(3), 406; https://doi.org/10.3390/rs18030406 - 26 Jan 2026
Viewed by 626
Abstract
Mapping forest growth potential across varying environments is challenging, especially when field measurements are limited. In this study, we integrated Airborne Laser Scanning (ALS) terrain derivatives and Sentinel-2 spectral indices to model Site Index (SI), using forest plantations, at 10-m spatial resolution across [...] Read more.
Mapping forest growth potential across varying environments is challenging, especially when field measurements are limited. In this study, we integrated Airborne Laser Scanning (ALS) terrain derivatives and Sentinel-2 spectral indices to model Site Index (SI), using forest plantations, at 10-m spatial resolution across three ecologically distinct regions in British Columbia (Aleza Lake, Deception, and Eagle Hills). Random Forest regression models were calibrated using field-measured SI and a multistep variable-selection procedure that included Variance Inflation Factor (VIF) screening followed by model-based variable importance assessment. Model performance was evaluated using repeated 10-fold cross-validation. The combined ALS–Sentinel-2 models substantially outperformed single-source models, yielding cross-validated R2 values of 0.63, 0.44, and 0.56 for Aleza Lake, Deception, and Eagle Hills, respectively, compared with R2 values of 0.40, 0.40, and 0.46 for ALS-only models. Key predictors consistently included terrain metrics, such as the Topographic Position Index (TPI) and the Topographic Wetness Index (TWI), along with satellite-derived chlorophyll-sensitive indices including S2REP (Sentinel-2 red-edge position), MTCI (MERIS terrestrial chlorophyll), and GNDVI (Greenness Normalized Difference Vegetation Index). A general model using predictors common to all regions performed comparably (R2 = 0.63, 0.41, 0.52), demonstrating the transferability and operational potential of the approach. These findings demonstrate that integrating ALS-derived terrain metrics with Sentinel-2 spectral indices provides a robust, age-independent framework for capturing spatial variability in forest productivity across landscapes. This multi-sensor fusion approach enhances traditional SI methods and single-sensor models, providing a scalable and operational tool for forest management and long-term planning in changing environmental conditions. Full article
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23 pages, 8140 KB  
Article
Comparative Assessment of Hyperspectral and Multispectral Vegetation Indices for Estimating Fire Severity in Mediterranean Ecosystems
by José Alberto Cipra-Rodriguez, José Manuel Fernández-Guisuraga and Carmen Quintano
Remote Sens. 2026, 18(2), 244; https://doi.org/10.3390/rs18020244 - 12 Jan 2026
Viewed by 591
Abstract
Assessing post-fire disturbance in Mediterranean ecosystems is essential for quantifying ecological impacts and guiding restoration strategies. This study evaluates fire severity following an extreme wildfire event (~28,000 ha) in northwestern Spain using vegetation indices (VIs) derived from PRISMA hyperspectral imagery, validated against field-based [...] Read more.
Assessing post-fire disturbance in Mediterranean ecosystems is essential for quantifying ecological impacts and guiding restoration strategies. This study evaluates fire severity following an extreme wildfire event (~28,000 ha) in northwestern Spain using vegetation indices (VIs) derived from PRISMA hyperspectral imagery, validated against field-based Composite Burn Index (CBI) measurements at the vegetation, soil, and site levels across three vegetation formations (coniferous forests, broadleaf forests, and shrublands). Hyperspectral VIs were benchmarked against multispectral VIs derived from Sentinel-2. Hyperspectral VIs yielded stronger correlations with CBI values than multispectral VIs. Vegetation-level CBI showed the highest correlations, reflecting the sensitivity of most VIs to canopy structural and compositional changes. Indices incorporating red-edge, near-infrared (NIR), and shortwave infrared (SWIR) bands demonstrated the greatest explanatory power. Among hyperspectral indices, DVIRED, EVI, and especially CAI performed best. For multispectral data, NDRE, CIREDGE, ENDVI, and GNDVI were the most effective. These findings highlight the strong potential of hyperspectral remote sensing for accurate, scalable post-fire severity assessment in heterogeneous Mediterranean ecosystems. Full article
(This article belongs to the Section Forest Remote Sensing)
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18 pages, 4523 KB  
Article
Remote Sensing of Nematode Stress in Coffee: UAV-Based Multispectral and Thermal Imaging Approaches
by Daniele de Brum, Gabriel Araújo e Silva Ferraz, Luana Mendes dos Santos, Felipe Augusto Fernandes, Marco Antonio Zanella, Patrícia Ferreira Ponciano Ferraz, Willian César Terra, Vicente Paulo Campos, Thieres George Freire da Silva, Ênio Farias de França e Silva and Alexsandro Oliveira da Silva
AgriEngineering 2026, 8(1), 22; https://doi.org/10.3390/agriengineering8010022 - 8 Jan 2026
Cited by 1 | Viewed by 772
Abstract
Early and non-destructive detection of plant-parasitic nematodes is critical for implementing site-specific management in coffee production systems. This study evaluated the potential of unmanned aerial vehicle (UAV) multispectral and thermal imaging, combined with textural analysis, to detect Meloidogyne exigua infestation in Coffea arabica [...] Read more.
Early and non-destructive detection of plant-parasitic nematodes is critical for implementing site-specific management in coffee production systems. This study evaluated the potential of unmanned aerial vehicle (UAV) multispectral and thermal imaging, combined with textural analysis, to detect Meloidogyne exigua infestation in Coffea arabica (Topázio variety). Field surveys were conducted in two contrasting seasons (dry and rainy), and nematode incidence was identified and quantified by counting root galls. Vegetation indices (NDVI, GNDVI, NGRDI, NDRE, OSAVI), individual spectral bands, canopy temperature, and Haralick texture features were extracted from UAV-derived imagery and correlated with gall counts. Under the conditions of this experiment, strong correlations were observed between gall number and the red spectral band in both seasons (R > 0.60), while GNDVI (dry season) and NGRDI (rainy season) showed strong negative correlations with gall density. Thermal imaging revealed moderate positive correlations with infestation levels during the dry season, indicating potential for early stress detection when foliar symptoms were absent. Texture metrics from the red and green bands further improved detection capacity, particularly with a 3 × 3 pixel window at 135°. These results demonstrate that UAV-based multispectral and thermal imaging, enhanced by texture analysis, can provide reliable early indicators of nematode infestation in coffee. Full article
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24 pages, 5699 KB  
Article
Alpine Grassland Growth and Its Ecological Responses to Environmental Impacts: Insights from a Comprehensive Growth Index and SHAP-Based Analysis
by Yanying Li, Yongmei Liu, Xiaoyu Li, Junjuan Yan, Yuxin Du, Ying Meng and Jianhong Liu
Plants 2026, 15(1), 93; https://doi.org/10.3390/plants15010093 - 27 Dec 2025
Viewed by 656
Abstract
The alpine grassland is one of the most representative ecosystems on the Qinghai–Tibet Plateau, Growth monitoring is fundamental for the alpine grassland maintenance and husbandry sustainability. In this study, by the integration of regression model, principal component analysis, and SHAP-enhanced machine learning, a [...] Read more.
The alpine grassland is one of the most representative ecosystems on the Qinghai–Tibet Plateau, Growth monitoring is fundamental for the alpine grassland maintenance and husbandry sustainability. In this study, by the integration of regression model, principal component analysis, and SHAP-enhanced machine learning, a comprehensive growth index (CGI) was proposed for the accurate and quick assessment of alpine grassland growth in Qinghai Province, located in the eastern Qinghai–Tibet Plateau. The temporal and spatial growth behaviors of the main grassland types over 2001–2023 were then determined and the differences in key driving factors and their responses explored. The results indicated that the CGI composed of KNDVI, EVI, MSAVI, GNDVI and CVI characterized the typical ecological and physical parameters related to grassland growth, proved to be optimal and efficient in long-term growth monitoring. Alpine grassland growth fluctuated but gradually increased from 2001 to 2023, but individual types exhibited different trends. In particular, the two main types of alpine meadow and alpine steppe displayed the weakest increasing trend in growth, with the good-growth and continuous-increasing area proportions of 26.01% and 18.03%, 70.45% and 74.72%, respectively. Soil total nitrogen was the most critical common factor and significantly increased the growth across all five grassland types, then followed by grazing intensity and precipitation, which exhibits diverse effects on the individual types. The result implies the significant heterogeneity in the key driviers which affect the alpine grassland growth over large scale. Full article
(This article belongs to the Section Plant Ecology)
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17 pages, 1254 KB  
Article
Remote Monitoring of Coffee Leaf Miner Infestation Using Fuzzy Logic and the Google Earth Engine Platform
by Laura Teixeira Cordeiro, Emerson Ferreira Vilela, Jéssica Letícia Abreu Martins, Charles Cardoso Santana, Filipe Schitini Salgado, Gislayne Farias Valente, Diego Bedin Marin, Christiano de Sousa Machado Matos, Rogério Antônio Silva, Margarete Marin Lordelo Volpato and Madelaine Venzon
AgriEngineering 2025, 7(12), 435; https://doi.org/10.3390/agriengineering7120435 - 16 Dec 2025
Viewed by 799
Abstract
The coffee leaf miner (Leucoptera coffeella) is a major pest of coffee crops and can cause significant economic losses. Early monitoring is essential to support decision-making for its control. This study aimed to evaluate the potential of fuzzy logic for detecting [...] Read more.
The coffee leaf miner (Leucoptera coffeella) is a major pest of coffee crops and can cause significant economic losses. Early monitoring is essential to support decision-making for its control. This study aimed to evaluate the potential of fuzzy logic for detecting leaf miner infestation using a 2.5-year historical series of Sentinel-2A satellite images processed on the Google Earth Engine platform. Field monitoring of coffee leaf miner infestation was carried out at the EPAMIG Experimental Field, located in São Sebastião do Paraíso, Minas Gerais, Brazil. The period evaluated was from September 2022 to April 2025. Vegetation indices were calculated using the Google Earth Engine platform, and a database was built with eight indices (NDVI, EVI, GNDVI, SR, IPVI, NDMI, MCARI, and CLMI) along with coffee leaf miner infestation data. Principal Component Analysis (PCA) was applied to reduce data dimensionality and identify the most relevant indices for distinguishing infested from healthy plants, explaining 90.9% of the total variance in the first two components (PC1 and PC2). The indices CLMI, IPVI, GNDVI, and MCARI showed the greatest contribution to class separation. A fuzzy inference model was implemented based on the mean index values and validated through performance metrics. The results indicated an overall accuracy of 79.1%, a sensitivity (recall) of 86.6%, a specificity of 66.6%, an F1-score of 0.838, a Kappa coefficient of 0.545, and an area under the curve (AUC) of 0.766. These findings confirm the potential of integrating orbital spectral data via Google Earth Engine with fuzzy logic analysis as an efficient tool, contributing to the adoption of more sustainable monitoring practices in coffee farming. The fuzzy logic system received as input the spectral values derived from Sentinel-2A imagery, specifically the indices identified as most relevant by the PCA (CLMI, IPVI, GNDVI, and MCARI). These indices were computed and integrated into the inference model through processing routines developed in the Google Earth Engine platform, enabling a direct connection between satellite-derived spectral patterns and the detection of coffee leaf miner infestation. Full article
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32 pages, 2403 KB  
Review
Vegetation Indices from UAV Imagery: Emerging Tools for Precision Agriculture and Forest Management
by Adrian Peticilă, Paul Gabor Iliescu, Lucian Dinca, Andy-Stefan Popa and Gabriel Murariu
AgriEngineering 2025, 7(12), 431; https://doi.org/10.3390/agriengineering7120431 - 14 Dec 2025
Cited by 3 | Viewed by 2163
Abstract
Unmanned Aerial Vehicles (UAVs) have become essential instruments for precision agriculture and forest monitoring, offering rapid, high-resolution data collection over wide areas. This review synthesizes global advances (2015–2024) in UAV-derived vegetation indices (VIs), combining bibliometric and content analyses of 472 peer-reviewed publications. The [...] Read more.
Unmanned Aerial Vehicles (UAVs) have become essential instruments for precision agriculture and forest monitoring, offering rapid, high-resolution data collection over wide areas. This review synthesizes global advances (2015–2024) in UAV-derived vegetation indices (VIs), combining bibliometric and content analyses of 472 peer-reviewed publications. The study identifies key research trends, dominant indices, and technical progress achieved through RGB, multispectral, hyperspectral, and thermal sensors. Results show an exponential growth of scientific output, led by China, the USA, and Europe, with NDVI, NDRE, and GNDVI remaining the most widely applied indices. New indices such as GSI, RBI, and MVI demonstrate enhanced sensitivity for stress and disease detection in both crops and forests. UAV-based monitoring has proven effective for yield prediction, water-stress evaluation, pest identification, and biomass estimation. Despite significant advances, challenges persist regarding illumination correction, soil background influence, and limited forestry applications. The paper concludes that UAV-derived vegetation indices—when integrated with machine learning and multi-sensor data—represent a transformative approach for the sustainable management of agricultural and forest ecosystems. Full article
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Article
Machine Learning Approaches for Assessing Avocado Alternate Bearing Using Sentinel-2 and Climate Variables—A Case Study in Limpopo, South Africa
by Muhammad Moshiur Rahman, Andrew Robson and Theo Bekker
Remote Sens. 2025, 17(24), 3935; https://doi.org/10.3390/rs17243935 - 5 Dec 2025
Viewed by 1247
Abstract
Alternate (irregular) bearing, characterized by large fluctuations in fruit yield between consecutive years, remains a major constraint to sustainable avocado (Persea americana) production. This study aimed to assess the potential of satellite remote sensing and climatic variables to characterize and predict [...] Read more.
Alternate (irregular) bearing, characterized by large fluctuations in fruit yield between consecutive years, remains a major constraint to sustainable avocado (Persea americana) production. This study aimed to assess the potential of satellite remote sensing and climatic variables to characterize and predict alternate bearing patterns in commercial orchards in Tzaneen, Limpopo Province, South Africa. Historical yield data (2018–2024) from 46 “Hass” avocado blocks were analyzed alongside Sentinel-2 derived vegetation indices (NDVI, GNDVI, NDRE, CIG, CIRE, EVI2, LSWI) and flowering indices (WYI, NDYI, MTYI). To align temporal scales, all VIs and FIs were aggregated into eight quarterly averages from the two years preceding each yield year and spatially averaged across each orchard block. Climatic predictors including maximum temperature (Tmax), minimum temperature (Tmin), vapor pressure deficit (VPD), and precipitation were screened against historical yields to identify critical periods, with June–October emerging as the most influential months, and these variables were aggregated accordingly to match annual alternate bearing patterns. Five machine learning (ML) algorithms—Random Forest, XGBoost, CATBoost, LightGBM, and TabPFN—were trained and tested using a Leave-One-Year-Out (LOYO) approach. Results showed that VPD, Tmin, and Tmax during the flowering period (July–September) were the most influential variables affecting subsequent yields. TabPFN achieved the highest predictive accuracy (Accuracy = 0.88; AUC = 0.95) and strongest temporal generalization. Spectral gradients between flowering and early fruit drop were lower during “on” years, reflecting stable canopy vigor. This combined use of remote sensing and climatic variables in a ML framework represents a novel approach, and the findings demonstrate that integrating remote sensing and climatic indicators enables early discrimination of “on” and “off” years, supporting proactive orchard management and improved yield stability. Full article
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