Search Results (81)

This study evaluated how different additives—Lactiplantibacillus plantarum (LP), Lentilactobacillus buchneri (LB), and a composite enzyme (CE)—affect the fermentation quality, nutritional value, and microbial community of Leymus chinensis silage. Fresh forage was wilted to 65% moisture, treated with additives (dissolved in distilled water), and vacuum-sealed in polyethylene bags for 60 days of ensiling. Fermentation parameters and nutritional composition were analyzed using standard methods (e.g., HPLC for organic acids, Kjeldahl for crude protein), and the microbial community was profiled via Illumina MiSeq sequencing of the 16S rRNA gene V3-V4 region. Data were subjected to one-way ANOVA and Duncan’s test in SAS. All additives significantly improved key fermentation parameters (p < 0.05). The LP treatment yielded the most favorable profile, with the lowest pH (4.26) and the highest lactic acid (6.52 g/kg DM) and acetic acid (2.58 g/kg DM) contents. LP also best preserved nutrients, showing the highest dry matter (581.62 g/kg FW), water-soluble carbohydrates (24.76% g/kg DM), and crude protein (7.09% DM) (p < 0.05). The CE treatment most effectively degraded fiber, resulting in the lowest acid detergent fiber (428.87% g/kg DM) and neutral detergent fiber (628.43% g/kg DM) (p < 0.05). Additives significantly reduced bacterial alpha-diversity but enriched beneficial phylum such as Bacillota and genus such as Lentilactobacillus spp. LB), while suppressing harmful genera. Correlation analysis confirmed LP was positively correlated with lactic acid and water-soluble carbohydrates (p < 0.0001). In conclusion, additives, particularly LP, enhance silage quality by modulating the microbial community. Full article

This research aimed to establish reference intervals and evaluate the influence of age in 56 Nguni cattle raised in Mozambique. Blood samples containing EDTA as anticoagulant were collected. The erythrogram and total leukocyte count were analyzed using the BC-2800 Vet Mindray^® automatic counter. The differential counting of leukocytes was performed in blood smears stained using Giemsa and Mcgruwald’s stain. Statistical analysis was performed using the statistical analysis system (SAS). Analysis of variance was performed using the GLM procedure, and mean contrasts were analyzed using Duncan’s parametric test at 5% significance, with the Shapiro–Wilk and Levene tests for date normalities. The reference intervals for the erythrogram are as follows: red blood cells: 6.78 to 7.40 × 10¹²/L; hemoglobin: 10.77 to 11.36 g/dL; hematocrit: 28.02 to 29.56%; mean corpuscular volume (MCV): 39.91 to 43.02 fL; mean corpuscular hemoglobin (MCH): 15.27 to 16.44 pg; mean corpuscular hemoglobin concentration (MCHC): between 37.86 and 39.10 g/dL; and red blood cell distribution width (RDW): between 16.98 and 19.40%. For leukograms, the following references values were obtained: total leukocytes: between 14,106 and 16,233 × 10⁶/L; basophils: between 32 and 165 × 10⁶/L; eosinophils: between 823 and 1262 × 10⁶/L; band neutrophils: between 25 and 87 × 10⁶/L; segmented neutrophils: between 2510 and 3249 × 10⁶/L; total neutrophils: between 2565 and 3306 × 10⁶/L; lymphocytes: between 9471 and 11,474 × 10⁶/L; and monocytes: between 154 and 296 × 10⁶/L. Age influenced MCV, MCH, leukocytes, eosinophils, and lymphocytes. Leukogram reference intervals of other countries could not be used for the breed of Mozambique without making gross errors. Full article

Polyaspartic acid (PASP), a biodegradable and eco-friendly fertilizer synergist that shows potential to enhance nutrient use efficiency in agricultural systems, has its integrative role with rhizosphere microorganisms remain insufficiently explored. This study integrated outdoor pot experiments, soil biochemical analysis, and microbiome sequencing to investigate the effects of co-application of PASP and the plant growth-promoting rhizobacterium (PGPR) Enterobacter asburiae S13 on potato growth, with four treatments set up including blank control (CK), sole application of PASP (S0P1), sole inoculation of PGPR (S1P0), and co-application of PASP and PGPR (S1P1), and 25 pots per treatment as replicates. The results showed that, compared with the S0P1 treatment, the S1P1 treatment significantly increased plant height (9.59%), stem diameter (28.39%), root length (38.61%), as well as root and shoot biomass (21.26% and 25.17%, respectively) (ANOVA, Duncan’s test, p < 0.05). It also enhanced ammonium nitrogen (40.00%), nitrate nitrogen (57.70%), available potassium (47.56%), and urease activity in the rhizosphere soil (ANOVA, Duncan’s test, p < 0.05). 16S rRNA sequencing revealed that the S1P1 treatment enriched beneficial taxa such as Paucibacter and Massilia, while suppressing competitive genera such as Duganella and Pedobacter. Redundancy analysis (RDA) indicated that available potassium and ammonium nitrogen were the key factors shaping the microbial community structure. In conclusion, combining PASP with PGPR synergistically improves soil nutrient availability and reshapes the rhizosphere microbiome, resulting in enhanced potato growth, thus demonstrating its potential as a dual-function biostimulant for eco-efficient and sustainable potato production systems. Full article

Urban and peri-urban farmers in Malawi increasingly use treated and untreated wastewater for vegetable production, but little is known about the extent of heavy metal accumulation in both exotic and indigenous vegetables, particularly with respect to differences between edible tissues (leaves vs. stems). This study addresses this gap by measuring the concentrations of cadmium (Cd), chromium (Cr), lead (Pb), zinc (Zn), and copper (Cu) in wastewater, soils, and six vegetables including three exotic and three indigenous irrigated with effluent from the Soche Wastewater Treatment Plant in Blantyre. Metal concentrations were determined using Atomic Absorption Spectrophotometry. Wastewater contained Zn (0.01 ± 0.001 mg/L) and Cu (0.02 ± 0.018 mg/L), both below World Health Organization (WHO) and Malawi Bureau of Standards (MBS) limits (Zn: 0.2 mg/L; Cu: 2 mg/L), while Cd, Cr, and Pb were below detection limit. In soils, Zn reached 56.4 ± 0.5 mg/kg, exceeding the WHO limit of 36 mg/kg; other metals remained within WHO permissible values. Vegetables showed species- and tissue-specific variation in metal accumulation: Cr reached 4.65 mg/kg in Cucurbita moschata stems, Cd up to 0.31 mg/kg in Amaranthus retro-flexus leaves, and Pb up to 4.09 mg/kg in Brassica rapa stems—all above FAO/WHO permissible limits (2.3, 0.2, and 0.3 mg/kg, respectively). Duncan’s post hoc analysis confirmed significant differences (p < 0.05) across matrices and plant parts, with leaves generally accumulating more Zn and Cu than stems. Principal component analysis (PCA) revealed that Zn, Cu, Cr, and Pb in the wastewater-soil-vegetable system largely share a common source, likely wastewater effluent and historical soil contamination, while Cd showed a more sporadic distribution, highlighting differential accumulation pathways. Health risk assessments revealed high Health Risk Index (HRI) values, with Brassica rapa stems (HRI = 92.3) and Brassica rapa subsp. chinensis leaves (HRI = 82.2) exceeding the safe threshold (HRI > 1), indicating potential chronic risks. This study reveals potential health risks associated with wastewater irrigation due to heavy metal accumulation in edible vegetables, and therefore recommends further research on metal speciation, seasonal variation, and bioaccumulation at different crop growth stages. Full article

This study evaluated the suitability of three floodplain environments near Iquitos for cowpea cultivar recommendations and estimated the adaptability and phenotypic stability of 12 cowpea lines evaluated in 2004. Climatic conditions between 2004 and 2020–2024 were also compared. Three field trials used a randomized complete block design with 12 lines and two replications, assessing grain yield, number of pods per plant, days to flowering, days to 50% maturity, and days to harvest. Combined Analysis of Variance (ANOVA), Duncan’s means test, Genotype + Genotype Environment Interaction (GGE) biplot, and Additive Main Effects and Multiplicative Interactions Interaction (AMMI) analyses revealed that the three sites are contrasting. The Annicchiarico index, GGE biplot, and AMMI analyses identified line CAR 3010 as having superior adaptability and stability. Paired t-tests and Mann–Kendall analyses showed that climatic conditions in 2020–2024 differed significantly from 2004. Therefore, Muyuy, Rafael Belaunde, and San Miguel are suitable locations for testing advanced cowpea lines prior to cultivar recommendation. Line CAR 3010 is recommended for breeding programs in the Peruvian Amazon in response to ongoing climate change. This research addresses a critical knowledge gap as the first study evaluating cowpea adaptability and stability across multiple floodplain locations in the Peruvian Amazon. Full article

Background/Objectives: Selenoproteins play indispensable roles in embryonic development, with their dysregulation linked to various metabolic and neurological disorders. This study aims to systematically quantify the mRNA expression levels of all 24 selenoprotein genes in murine heart, brain, liver, and kidney tissues across embryonic (E8.5, E12.5, E18.5) and postnatal (P7, P30, P90) developmental stages, in order to elucidate the regulatory landscape of selenium metabolism during development. Methods: We collected tissues from mice at six developmental stages and performed RNA extraction followed by quantitative real-time PCR (qPCR) to measure the expression of all 24 selenoprotein genes. Data were normalized using the geometric mean of ActB and Gapdh, and statistical analyses were conducted using one-way ANOVA with Duncan’s post hoc test. Results: Our analysis reveals three principal findings: (1) Distinct expression patterns emerge among selenoprotein families—deiodinases (Dio1-3) and thioredoxin reductases (Txnrd1-3) exhibit limited embryonic expression (<20-fold changes), while glutathione peroxidases (Gpx1, Gpx3, Gpx4) and biosynthesis-related genes (Selenop, Msrb1) show substantial postnatal upregulation (up to 600-fold increases); (2) Selenoproteins essential for embryonic survival (Gpx4, Txnrd1, Txnrd2, Selenoi, Selenot) display expression profiles concordant with their essential developmental functions; (3) Selenop and Msrb1, involved in selenium transport and redox regulation, demonstrate early embryonic upregulation with further increases during postnatal development. Conclusions: These spatiotemporal expression patterns elucidate the regulatory landscape of selenium metabolism during development and provide mechanistic insights into the phenotypes associated with selenium deficiency. The findings offer valuable implications for human nutritional interventions and developmental health. Full article

The reliability analysis of slope stability is significantly influenced by the variance of the soil’s shear strength. Currently, the shear strength of coarse-grained soil is commonly determined using the Duncan formula, which establishes a relationship between the shear strength and confining pressure. Specifically, the parameters of the Duncan formula are estimated based on available test data using least squares regression, which mitigates the limitations associated with small sample sizes and significant errors in the classic grouped data method. However, hypothesis testing in mathematical statistics reveals that the residuals from the classic least squares estimation exhibit heteroscedasticity and correlation, violating the fundamental assumptions required for least squares regression. Consequently, parameter estimates obtained through classic least squares regression have large variances, leading to unreliable statistical inferences. To address this issue, we propose a generalized least squares method that eliminates the heteroscedasticity and correlation of the residuals. Triaxial test data for five different coarse-grained soils are analyzed using the proposed method. The results show that the mean values of the estimated parameters of the Duncan formula are close to those obtained using the classic method, while their variances are significantly reduced, demonstrating the effectiveness of the proposed approach. The reliability analysis of the anti-sliding stability of an infinitely long slope shows that the strength parameters estimated by the classical least squares method tend to underestimate the stability of the slope due to the large variance. Therefore, the Duncan nonlinear shear strength parameters of coarse-grained soils should be estimated using the generalized least squares method that eliminates the heteroscedasticity and correlation of the regression residuals. Full article

Although the upper nodal roots are vital for resource uptake in late-stage maize, their anatomical and physiological responses to varying nitrogen and light regimes remain unclear. In a field experiment, maize was grown under three nitrogen levels (0, 192, and 240 kg·ha⁻¹) and two light regimes (normal light and 50% shading). At flowering (R1), we selected the number, diameter, anatomy of nodal roots, root-bleeding sap composition, and grain yield. Sample sizes ranged from three to twelve replicates per treatment, depending on the trait measured. Data were analyzed using ANOVA and Duncan’s test (p < 0.05). Under normal light, N₁₉₂ and N₂₄₀ significantly enhanced stele and vessel diameters in the sixth and seventh nodal root whorls, vessel number and cortical cell layers in the fifth and seventh whorls, root-bleeding intensity, exudation rates of sucrose, abscisic acid, key free amino acids (Asn, Asp, Glu), and grain yield, compared to N₀. Shading markedly suppressed the nodal root anatomical structure, reducing root-bleeding intensity by 18.2–26.6% and yield by 30.6–40.8%; especially under SS-N0, which also notably increased the exudation of stress-related amino acids (particularly Asp and Glu). Correlation analysis revealed positive relationships of root-bleeding intensity with vessel area and grain yield, indicating that impaired root anatomy restricts resource transport under shading. Sufficient nitrogen partially alleviated these adverse effects. This study demonstrates that light and nitrogen synergistically regulate the upper nodal root anatomy, thereby modulating root-bleeding sap and ultimately influencing grain yield. These results provide a theoretical basis for high-yield maize cultivation and precision nitrogen management under low-light stress. Full article

This study is focused on the analysis of the level of geometric thinking of 15-year-old Slovak pupils in relation to the difficulty of geometric problems, their gender, and their assessment in mathematics. The main aim of this study was to determine the level of geometric thinking of 15-year-old Slovak pupils, to examine the relationship between their mathematics assessment and the level of geometric thinking, and to find out gender differences in relation to the different levels of geometric thinking. The van Hiele test was adapted and applied to a representative sample of 15-year-old Slovak pupils to determine the level of geometric thinking. We used reliability/item analysis. The reliability of the knowledge test (after adaptation) was assessed using Cronbach’s alpha (0.64). The validity of the test was demonstrated by the correlation of the Usiskin test results with pupils’ mathematics grades (Goodman–Kruskal’s gamma, p < 0.05). Statistical analysis showed that 15-year-old Slovak pupils achieve different levels of geometric thinking depending on the difficulty of the tasks. Pupil achievement declined significantly as task difficulty increased. Pupils had the greatest difficulty with tasks classified as the fifth (rigorous) and partly the fourth (deductive) van Hiele level, which require a deep understanding of geometric systems and the ability to prove logically. The lower-level tasks (visualization, analysis, and abstraction) were able to differentiate students according to different levels of geometric thinking. The results showed a significant positive relationship (Goodman–Kruskal’s gamma, p < 0.05) between the pupils’ overall mathematics scores (expressed as a grade) and their level of geometric thinking as detected by the van Hiele test. The analysis of gender differences (Duncan’s test, p < 0.05) showed that in the less challenging tasks, corresponding to the first three van Hiele levels (visualization, analysis, abstraction), girls performed statistically significantly better than boys. In the more challenging tasks, classified as the fourth (deductive) and fifth (rigorous) levels of geometric thinking, there were no statistically significant differences between boys and girls. In the more challenging tasks, the performances of both genders were comparable. The presented study identifies significant deficits in the development of higher levels of geometric thinking among 15-year-old Slovak pupils. These findings strongly imply the necessity for the transformation of the curriculum, textbooks, and didactic approaches with the aim of systematically developing deductive and rigorous reasoning, while it is essential to account for the demonstrated gender differences in performance. Full article

Under the ideas of ‘hinges’ and ‘pictures’, as these relate to deep disagreement, Wittgenstein’s view of religious belief is a multifaceted challenge to conceptions of thought-world relations. In this article, I discuss Cora Diamond’s analysis of this challenge. Diamond herself is not particularly interested in hinges; I try to understand why. I first bring in a discussion between Michael Williams and Duncan Pritchard on how to read On Certainty. This allows me to identify Diamond’s perspective on deep disagreement and pictures: she concentrates on making sense, and not directly on knowledge. To further clarify her perspective, I introduce Hilary Putnam’s reading of the Lectures on Religious Belief, which proposes a cognitivist view of religion as ethics, centering on the notion of picture. Although Diamond is close to Putnam, for her, the most important challenge posed by religious belief lies not with epistemological issues of rational versus arational grounds of belief, or cognitivism versus non-cognitivism in ethics, but rather in making us drop the Fregean (and Tractarian) idea of the thinkableness of all thoughts, making place for ‘irreplaceable pictures’. I end by suggesting that Diamond’s analysis sheds light on often uncontested assumptions about the natures of thought and communication. Full article

The present study analyzed the vegetation diversity in three forests located in southern Romania and assessed their degree of similarity. Data were collected using frame quadrat sampling and species taxonomic identification. The methodology included the calculation of ecological indices (Shannon–Wiener, equitability, maximum entropy, Menhinick, Margalef, McIntosh, Gleason, and Simpson) and statistical analysis using ANOVA and Duncan tests (p < 0.05). Similarity between forests was evaluated using the Jaccard and Dice/Sørensen coefficients. The results showed that biodiversity increases with area size, and the forest ecosystem in Vlădila exhibited the highest number of woody and herbaceous species. Although the forest ecosystem in Studinița had the greatest floristic diversity, according to the Shannon–Wiener index, it also showed higher equitability (0.911 compared to 0.673 in Vlădila) due to a more uniform species distribution. The forest ecosystem in Studinița acted as an intermediate zone between those in Grădinile and Vlădila. Variations in diversity among the three areas reflect ecological differences influenced by location-specific factors such as soil type, climatic conditions, and human interventions. This suggests that ecological conditions and the physical characteristics of forests significantly impact the number and types of species that can coexist within an ecosystem. Full article

Alkali-activated binders (AAB) are a suitable and sustainable alternative to ordinary Portland cement (OPC), with reductions in natural resource usage and environmental emissions in regions where the necessary industrial residues are available. Despite its potential, the lack of mix design methods still limits its applications. This paper proposes a systematic parametric validation for AAB mix design applied to pastes and concretes, valorizing steel slag as precursors. The composed binders are based on coal fly ash (FA) and Basic Oxygen Furnace (BOF) steel slag. These precursors were activated with sodium silicate (Na₂SiO₃) and sodium hydroxide (NaOH) alkaline solutions. A parametric investigation was performed on the mix design parameters, sweeping the (i) alkali content from 6% to 10%, (ii) silica modulus (SiO₂/Na₂O) from 0.75 to 1.75, and (iii) ash-to-slag ratios in the proportions of 75:25 and 50:50, using parametric intervals retrieved from the literature. These variations were analyzed using response surface methodology (RSM) to develop a mechanical model of the compressive strength of the hardened paste. Flowability, yield stress, and setting time were evaluated. Statistical analyses, ANOVA and the Duncan test, validated the model and identified interactions between variables. The concrete formulation design was based on aggregates packing analysis with different paste contents (from 32% up to 38.4%), aiming at self-compacting concrete (SCC) with slump flow class 1 (SF1). The influence of the curing condition was evaluated, varying with ambient and thermal conditions, at 25 °C and 65 °C, respectively, for the initial 24 h. The results showed that lower silica modulus (0.75) achieved the highest compressive strength at 80.1 MPa (28 d) for pastes compressive strength, densifying the composite matrix. The concrete application of the binder achieved SF1 fluidity, with 575 mm spread, 64.1 MPa of compressive strength, and 26.2 GPa of Young’s modulus in thermal cure conditions. These findings demonstrate the potential for developing sustainable high-performance materials based on parametric design of AAB formulations and mix design. Full article

The existing stability analysis of Coulomb retaining walls is derived on the basis of four assumptions, and there is no clear description of the interaction mechanism among wedge-shaped bodies, retaining walls, and the two. This article proposes a new method for calculating the stability of Coulomb retaining walls. For soil wedges and retaining walls, numerical theoretical solutions for the stress distribution in soil wedges and retaining walls were obtained on the basis of stress balance differential equations, coordination equations, force boundary conditions, and macroscopic equilibrium. The boundary condition between the soil wedge and the retaining wall is that the resultant force and moment between the two are continuous. Assuming that the soil wedge and retaining wall satisfy the Duncan–Zhang model and the linear elastic Hooke constitutive model, respectively, the strain solutions of the soil wedge and retaining wall are obtained. Assuming that both the peak strength criteria for the soil wedges and the concrete retaining walls satisfy the Mohr–Coulomb criterion, the location of the first point of failure for the soil wedges and retaining walls is determined. Taking the garbage transfer station in Lvcongpo Town, Badong County, as an example, the analysis of the force and displacement of the retaining wall and years of operation show that the proposed method for calculating the stress and strain of the retaining wall and the new method for evaluating the stability of the retaining wall based on the point strength criterion are feasible. Full article

With the growing demand for mobility fueled by global population expansion and rapid urbanization, the intricate interplay between public transport and land use, along with their economic, environmental, and social externalities, has emerged as a critical concern for policymakers and the public alike. This study assembles publicly available academic literature, including papers, reports, books, and news articles, to construct a comprehensive database. Using CiteSpace 5.8.R3 software, this study conducts a visualized analysis of 10,470 scholarly works on public transport and land use published since 1932, identifying and synthesizing the researcher, research stages, research theories, research models, and research hotspots. Findings reveal that since Mitchell and Rapkin first introduced the transport–land use relationship in 1954, research in this field has steadily gained traction, particularly after the 1973 oil embargo crisis. The Journal of Transport and Land Use and institutions such as the University of Minnesota’s Transportation Research Center have played pivotal roles, particularly with the establishment of the World Society for Transport and Land Use Research (WSTLUR). In recent years, China’s high-speed rail expansion has further revitalized interest in this field. Prominent scholars in this domain include Robert Cervero, Reid Ewing, Michael Duncan, and Peter Calthorpe. Major theoretical frameworks encompass utility theory, urban economic theory, and the human–land system theory. Key modeling approaches include the spatial interaction model, the stochastic utility model, and urban economic models. Current research hotspots center on safety and public health, equity and valuation, environmental sustainability and energy efficiency, as well as transit-oriented development (TOD) and accessibility. This systematic literature review offers valuable insights to inform land use planning, enhance spatial structure, guide transportation project decision making, and optimize transport infrastructure and service provision. Full article

The study of the compression characteristics of loess in seasonal regions involves analyzing the mechanical properties and mesoscale damage evolution of intact loess subjected to dry–wet freeze–thaw cycles. This study meticulously examines the evolution of the stress–strain curve at the macroscale and the pore structure at the mesoscale of loess by consolidation and drainage triaxial shear tests, as well as nuclear magnetic resonance (NMR), under varying numbers of dry–wet freeze–thaw cycles. Then, utilizing the Duncan–Chang model (D-C), the damage model for intact loess is derived based on the principles of equivalent strain and Weibull distribution, with testing to verify its applicability. The results indicate that the stress–strain curve of undisturbed loess exhibits significant strain softening during the initial stage of the freeze–thaw dry–wet cycle. As the number of cycles increases, the degree of strain softening weakens and gradually exhibits a strain-hardening morphology; the volume strain also changes from dilatancy to shear contraction. According to the internal pore test data analysis, the undisturbed loess contributes two components to shear strength: cementation and friction during the shear process. The cementation component of the aggregate is destroyed after stress application, resulting in a gradual enlargement of the pore area, evidenced by the change from tiny pores into larger- and medium-sized pores. After 10 cycles, the internal pore area of the sample expands by nearly 35%, indicating that the localized damage caused by the dry–wet freeze–thaw cycle controls the macroscopic mechanical properties. Finally, a damage constitutive model is developed based on the experimental phenomena and mechanism analysis, and the model’s validity is verified by comparing the experimental data with theoretical predictions. Full article