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Veterinary Sciences
Special Issues
Animal Clinical Pathology: Focus on Hematology and Biochemical Diagnostics
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Animal Clinical Pathology: Focus on Hematology and Biochemical Diagnostics

A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Biomedical Sciences".

Deadline for manuscript submissions: 10 September 2025 | Viewed by 3003

Special Issue Editor

Prof. Dr. Ana Cristina Silvestre Ferreira

E-Mail Website
Guest Editor
Department of Veterinary Sciences, Animal and Veterinary Research Centre (CECAV), University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal
Interests: immunohematology; transfusion medicine; feline blood types
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Animal clinical pathology is a crucial field in veterinary medicine that focuses on the diagnosis and treatment of diseases in animals through the analysis of blood and other body fluids. Hematology and biochemical diagnostics are two key components of animal clinical pathology that play a vital role in understanding the health status of animals.

Hematology involves the study of blood and its components, including red blood cells (RBCs), white blood cells (WBCs), and platelets. It is an essential tool with which veterinarians can assess the overall health of an animal and diagnose conditions such as anemia, infections, and clotting disorders. Hematology is also essential for monitoring the progression of diseases and evaluating the response to treatment.

Biochemical diagnostics, on the other hand, focuses on the analysis of biochemical markers in body fluids such as blood serum or plasma. These markers provide valuable information about the function of various organs and body systems.

Understanding hematology and biochemical diagnostics is crucial in the effective diagnosis and management of animal health. These tests provide valuable insights into the physiological and pathological states of animals, enabling timely and accurate medical interventions.

We invite researchers, clinicians, and experts in the field to contribute original research articles, reviews, and case studies that focus on all areas of animal clinical pathology.

Prof. Dr. Ana Cristina Silvestre Ferreira
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Veterinary Sciences is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • animal clinical pathology

  • hematology
  • biochemical diagnostics
  • veterinary medicine
  • disease diagnosis and treatment
  • biomarkers
  • coagulation
  • laboratory techniques

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Published Papers (2 papers)

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Research

19 pages, 783 KiB  
Article
Variants in Nucleotide Sequences; Gene Expression; and Hematological, Immune, and Antioxidant Biomarkers Linked to Pneumonia Risk in Holstein Calves
by Ahmed El-Sayed, Attia Eissa, Doaa Ebrahim, Ahmed Ateya, Hossam Gadalla, Hanan M. Alharbi, Khairiah M. Alwutayd, Manal A. Babaker and Aya Aly Elzeer
Vet. Sci. 2025, 12(7), 620; https://doi.org/10.3390/vetsci12070620 - 26 Jun 2025
Viewed by 588
Abstract
Pneumonia is a major issue that affects calves’ health and performance and causes numerous losses despite treatment. Investigating genetic and molecular differences, as well as immunological and antioxidant responses, in calves at risk for pneumonia was the aim of this study. A total [...] Read more.
Pneumonia is a major issue that affects calves’ health and performance and causes numerous losses despite treatment. Investigating genetic and molecular differences, as well as immunological and antioxidant responses, in calves at risk for pneumonia was the aim of this study. A total of 225 calves were studied, including 180 Holstein calves with respiratory signs and 45 calves that were apparently healthy. Blood samples were collected for CBC, RNA extractions, and immunological and antioxidant analysis. In contrast to the control group, the pneumonic one showed a considerable (p < 0.05) increase in the expression levels of cytokines and antioxidant genes IL1α, IL-1β, IL-6, IFN-γ, TNF-α, and NOX4. In contrast, the values of IL10, PRDX6, ATG7, and NDUFS6 were in the opposite range. The pneumonic and healthy calves were found to differ in the nucleotide sequences of the genes under analysis. In pneumonic calves, a substantial (p ˂0.05) reduction was detected in RBCs, Hb count, PCV%, and lymphocytes count, and a notable (p ˂ 0.05) increase in WBCs and neutrophil count was correlated with healthy control calves. The findings of the serum profile showed that there was a meaningful (p ˂ 0.05) rise in the serum values of IL-1α, IL-1β, IL-6, TNF-α, IFN-γ, and MDA, with significant reductions in the SOD, GSH, TAC, and IL-10 in the pneumonic compared to the healthy calves. Our results provide valuable information about the nucleotide sequence, gene expression, and serum profile differences of putative indicators for pneumonia in calves. This could be applied in monitoring calves’ pneumonia through the discriminate breeding of naturally resistant animals. Full article
(This article belongs to the Special Issue Animal Clinical Pathology: Focus on Hematology and Biochemical Diagnostics)
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7 pages, 225 KiB  
Article
Dog Blood Type DEA 1 in Two Municipalities of Luanda Province of Angola (Sub-Saharan Africa)
by Ana C. Silvestre-Ferreira, Hugo Vilhena, Ana C. Oliveira, José R. Mendoza, Maria Garcia Aura and Josep Pastor
Vet. Sci. 2024, 11(9), 449; https://doi.org/10.3390/vetsci11090449 - 22 Sep 2024
Viewed by 1224
Abstract
In dogs, the risk of an acute hemolytic transfusion reaction at the first transfusion is negligible; however, mismatched transfusions may produce alloimmunization. To avoid fatal acute hemolytic reactions in subsequent blood transfusions, it is important to recognize blood groups and to blood type [...] Read more.
In dogs, the risk of an acute hemolytic transfusion reaction at the first transfusion is negligible; however, mismatched transfusions may produce alloimmunization. To avoid fatal acute hemolytic reactions in subsequent blood transfusions, it is important to recognize blood groups and to blood type both the donor and the recipient. Prevalence of dog blood groups varies geographically and between breeds. Our aim was to determine DEA 1 prevalence in a canine population in Luanda (Angola) and to assess alloimmunization risk after a mismatched blood transfusion. Blood samples were typed using an immunochromatographic strip technique. Of the 112 dogs tested (59 males; 53 females), 52.68% were DEA 1 positive and 47.32% DEA 1 negative. Females tended to be DEA 1 positive, and males DEA 1 negative (p = 0.0085). In a first-time mismatched blood transfusion, the calculated probability of a dog becoming sensitized was 24.9% and the probability of an acute hemolytic reaction following a second incompatible blood transfusion was 6.21%. DEA 1 prevalence obtained was similar to that reported worldwide, but differs from other African countries. The risk of alloimmunization and acute hemolytic transfusion reactions in mismatched blood transfusions is higher than that in other African regions. Blood typing is recommended prior to transfusion. Full article
(This article belongs to the Special Issue Animal Clinical Pathology: Focus on Hematology and Biochemical Diagnostics)
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