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31 Results Found

  • Article
  • Open Access
643 Views
13 Pages

Micropropagation of a Bulgarian Local Form of Prunus mahaleb L. in Agar-Solidified Culture and PlantFormTM TIS Bioreactor

  • Rumyana Valkova,
  • Lilyana Nacheva,
  • Ivaylo Tsvetkov,
  • Athanasia Nikolaou and
  • Mariyana Gozmanova

Prunus mahaleb L. is an important cherry rootstock because of its tolerance to drought and poor soils. Despite its horticultural importance, efficient in vitro multiplication of P. mahaleb remains challenging. Therefore, this study compared conventio...

(This article belongs to the Collection Application of Tissue Culture to Horticulture)
  • Article
  • Open Access
3 Citations
2,257 Views
16 Pages

21 August 2025

Rosa canina is one of the precious native rose rootstocks with a high reputation among plant producers, which has potential horticultural and pharmacological properties related to the cosmetic values and the production of secondary metabolites. Due t...

(This article belongs to the Special Issue Plant Tissue Culture and Plant Regeneration—2nd Edition)
  • Article
  • Open Access
9 Citations
3,822 Views
17 Pages

Cistus creticus L. (rockrose), a species of ecological and medicinal significance, constitutes a valuable component of the Mediterranean ecosystem. The present study investigated the effect of the inorganic salt concentration of Murashige and Skoog m...

(This article belongs to the Special Issue Seed Germination and Micropropagation of Ornamental Plants)
  • Article
  • Open Access
7 Citations
2,521 Views
14 Pages

Effect of Adding De-Oiled Kitchen Water on the Bioconversion of Kitchen Waste Treatment Residue by Black Soldier Fly Larvae

  • Zhuojun He,
  • Cheng Yang,
  • Yan Peng,
  • Taoze Liu and ,
  • Zhanghong Wang and
  • Chengcai Xiong
  • + 1 author+ 0 author

With the continuous development of society, the output of kitchen waste (KW) is fast increasing. De-oiled kitchen water (DKW) and kitchen waste treatment residue (KWTR), two main by-products of the KW treatment industry, are produced accordingly on a...

(This article belongs to the Special Issue Solid Waste Treatment, Biohazards, and Management)
  • Review
  • Open Access
127 Citations
37,024 Views
28 Pages

The Past, Present and Future of Cannabis sativa Tissue Culture

  • Adrian S. Monthony,
  • Serena R. Page,
  • Mohsen Hesami and
  • Andrew Maxwell P. Jones

19 January 2021

The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect-/disease-/virus-fr...

(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)
  • Article
  • Open Access
3 Citations
3,795 Views
19 Pages

In the present study, the effects of different types of sugars and cultivation medium pH levels on the micropropagation of Krymsk 5® cherry rootstock were investigated. During the proliferation stage, the effects of four sugars (sucrose, fructose...

(This article belongs to the Section Crop Production)
  • Article
  • Open Access
17 Citations
6,881 Views
17 Pages

High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

  • Xiong Yang,
  • Xiaoyu Yang,
  • Ting Guo,
  • Kai Gao and ,
  • Tianyun Zhao,
  • Zhong Chen and
  • Xinmin An
  • + 2 authors+ 0 author

13 December 2018

Research Highlights: In the current study, we established a method for plant regeneration via somatic embryogenesis (SE) in Koelreuteria paniculata Laxm. for the first time. Background and Objectives: K. paniculata is an important ornamental and medi...

(This article belongs to the Section Forest Ecophysiology and Biology)
  • Article
  • Open Access
7 Citations
3,072 Views
15 Pages

In Vitro Propagation of Several Valuable Selections of Robinia pseudoacacia L. as a Fast and Sustainable Source for Wood Production

  • Ruben Budău,
  • Mariana Bei,
  • Cristian Onet,
  • Eliza Agud and ,
  • Olimpia Smaranda Mintas,
  • Adrian Ioan Timofte,
  • Cristina Adriana Rosan,
  • Vasile Laslo and
  • Simona Ioana Vicas
  • + 4 authors+ 0 author

25 October 2023

There is an increasing trend in forest production towards planting rapid-growing trees as attractive, environmentally friendly energy sources. This study aimed to establish an alternative to the traditional propagation of a number of selections of Ro...

(This article belongs to the Special Issue Advances in Sustainability Research from the University of Oradea)
  • Article
  • Open Access
13 Citations
6,451 Views
13 Pages

In Vitro Prevention of Browning in Persian Walnut (Juglans regia L.) cv. Sulaiman

  • Suhail Nazir Bhat,
  • Aroosa Khalil,
  • Nowsheen Nazir,
  • Mohammad Amin Mir and ,
  • Imran Khan,
  • Syed Shoaib Mubashir,
  • Mohammad Saleem Dar,
  • Shabir Hussain Wani and
  • Mohammad Anwar Hossain
  • + 4 authors+ 0 author

25 August 2022

The present investigation was undertaken to standardize the media and the anti-browning regime in order to minimize the phenolic browning of an in vitro culture of Persian walnut cv. Sulaiman. The experiments involved two types of explants, forced an...

  • Article
  • Open Access
14 Citations
5,280 Views
13 Pages

Potential and Constraints on In Vitro Micropropagation of Juniperus drupacea Labill.

  • Kostas Ioannidis,
  • Ioanna Tomprou,
  • Danae Panayiotopoulou,
  • Stefanos Boutsios and
  • Evangelia N. Daskalakou

12 January 2023

Juniperus drupacea Labill. (Cupressaceae) is a species with ecological and medicinal value. In Europe, it is native only in southern Greece, and is listed as endangered. Due to its uniqueness, this study attempted, for the first time, an in vitro pro...

(This article belongs to the Special Issue Application of Plant Biotechnology in Forestry)
  • Article
  • Open Access
15 Citations
7,483 Views
14 Pages

Establishment of an In Vitro Micropropagation System for Cannabis sativa ‘Cheungsam’

  • Sang-Cheol Baek,
  • Sang-Yoon Jeon,
  • Yoon-Jung Choi,
  • Bo-Hyun Byun and ,
  • Da-Hoon Kim,
  • Ga-Ram Yu,
  • Hyuck Kim and
  • Dong-Woo Lim
  • + 3 authors+ 0 author

Cannabis has been cultivated for thousands of years for a variety of purposes, including fiber, seeds, oil, and medicinal compounds. The cannabis industry is growing rapidly because several countries have recently legalized the use of cannabis. In th...

(This article belongs to the Special Issue Innovative Micropropagation of Horticultural and Medicinal Plants)
  • Article
  • Open Access
1 Citations
2,058 Views
15 Pages

5 September 2023

Catalpa bungei C. A. Mey is a unique and precious multi-purpose tree species that possesses great timber-related, ornamental and medicinal values. In this study, MS, N6 and DKW were used as basic media, and different concentrations of 6-BA and NAA we...

(This article belongs to the Special Issue Advances in Tree Germplasm Innovation and High-Efficiency Propagation)
  • Article
  • Open Access
8 Citations
2,633 Views
19 Pages

Groundwater Hydrogeochemical Processes and Potential Threats to Human Health in Fengfeng Coal Mining Area, China

  • Zhiqiang Zhang,
  • Haixue Li,
  • Fawang Zhang,
  • Jiazhong Qian and ,
  • Shuangbao Han and
  • Fenggang Dai
  • + 1 author+ 0 author

20 November 2023

The Fengfeng mining area is in the transition zone between the North China Plain and the Taihang Mountains, and groundwater is the main source of water supply in the district. Under the combined influence of human activities and natural geological co...

(This article belongs to the Special Issue Soil-Groundwater Pollution Investigations)
  • Article
  • Open Access
1 Citations
1,515 Views
17 Pages

Plant Regeneration via Somatic Embryogenesis in Juglans regia ‘Yunxin No. 14’

  • Jinwang Qu,
  • Xiurong Yang,
  • Linhe Xiang,
  • Bolin Wu and ,
  • Junzan Huang,
  • Chenyang Liang,
  • Aoao Cui,
  • Amenyogbe Mawuli Korsi,
  • Haigang Zhang and
  • Xinwu Xiong
  • + 7 authors+ 2 authors

The walnut cultivar ‘Yunxin No. 14’ is an early fruiting, high-yielding, and widely adaptable fruit tree with compact growth and superior nuts. Establishing a successful tissue culture system for this cultivar is crucial for its rapid clo...

(This article belongs to the Special Issue Innovative Tissue Culture Techniques for Sustainable Horticulture)
  • Article
  • Open Access
4 Citations
5,264 Views
13 Pages

An Efficient Agrobacterium-Mediated Genetic Transformation System for Persimmon (Diospyros kaki Thunb.)

  • Meng Zhang,
  • Sichao Yang,
  • Wenxing Chen,
  • Liqing Xu and ,
  • Dayong Guo,
  • Zhengrong Luo and
  • Qinglin Zhang
  • + 2 authors+ 0 author

Persimmon (Diospyros kaki Thunb.) is an important fruit crop in East Asia and has great commercial potential due to the high demand for fresh edible fruits or processed dried persimmon, as well as its being a source of raw materials for the chemical...

(This article belongs to the Section Genetics, Genomics, Breeding, and Biotechnology (G2B2))
  • Article
  • Open Access
15 Citations
4,082 Views
12 Pages

An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars

  • Vera Pavese,
  • Paola Ruffa,
  • Silvia Abbà,
  • Rita Lourenço Costa and ,
  • Elena Corredoira,
  • Cristian Silvestri,
  • Daniela Torello Marinoni and
  • Roberto Botta
  • + 3 authors+ 0 author

30 November 2022

Castanea sativa cv. ‘Garrone Rosso’ and ‘Marrone di Castel del Rio’ are two of the most prized varieties in Italy due to their valuable and healthy nuts used for fresh consumption and in the confectionery industry. Despite the...

(This article belongs to the Section Plant Molecular Biology)
  • Protocol
  • Open Access
1,455 Views
15 Pages

An Efficient High-Throughput Genetic Transformation Method for Hybrid Poplar

  • Gary Coleman,
  • Gen Li,
  • Reuben Tayengwa,
  • Simon Sretenovic and ,
  • Yiping Qi and
  • Edward Eisenstein
  • + 1 author+ 0 author

2 April 2026

A simple method for the efficient high-throughput transformation of hybrid poplar (Populus tremula x alba clone 717 1B) is described. Factors considered in developing the method included the ease and efficiency of preparing large numbers of explants...

(This article belongs to the Section Genetics and Molecular Biology)
  • Article
  • Open Access
23 Citations
5,672 Views
14 Pages

7 January 2023

Difficult-to-root plants often perform poorly during acclimatization and in vitro rooting can increase the survival and quality of plants. The influence of auxin application and mineral nutrition on in vitro rooting and subsequent effects on plant qu...

(This article belongs to the Special Issue Micropropagation and Cryopreservation of Plants)
  • Article
  • Open Access
22 Citations
6,341 Views
21 Pages

8 February 2022

American chestnut (Castanea dentata), a native species of eastern North America, is an economically important deciduous hardwood tree that has been designated as endangered in Canada. The population of American chestnut trees has dwindled significant...

(This article belongs to the Special Issue In Vitro Conservation of Endangered and Value-Added Plant Species)
  • Article
  • Open Access
6 Citations
3,678 Views
11 Pages

19 March 2025

Blue and supplemental far-red light were observed to affect in vitro shoot growth with Cannabis sativa (‘BaOx’ and ‘Cherry 1’) in RV750 vessels. A modified “hedging” and fed-batch system for multiple harvests using...

(This article belongs to the Section Plant Development and Morphogenesis)
  • Article
  • Open Access
921 Views
20 Pages

‘Korlaxiangli’ pear occupies a pivotal position in the pear industry of Xinjiang, with both its cultivation area and total output ranking first in the region. However, ‘Korlaxiangli’ is vulnerable to freezing injury and has su...

(This article belongs to the Section Propagation and Seeds)
  • Article
  • Open Access
3 Citations
6,191 Views
12 Pages

Natural populations of bigleaf maple (Acer macrophyllum Pursh) trees contain, at low frequency, individuals with stems that have attractive and valuable wavy grain in the wood. To maintain the genotype of these individuals, vegetative propagation is...

(This article belongs to the Section Propagation and Seeds)
  • Article
  • Open Access
8 Citations
3,229 Views
14 Pages

This study developed an efficient protocol for the in vitro propagation of giant reed (Arundo donax L.) biomass, defining a complete cycle of the induction of somatic embryogenesis from immature inflorescences, followed by the maturation of somatic e...

(This article belongs to the Special Issue In Vitro Propagation and Biotechnology of Horticultural Plants)
  • Article
  • Open Access
5 Citations
3,215 Views
21 Pages

Development of a Micropropagation Protocol for the Ex Situ Conservation of Nuttall’s Scrub Oak (Quercus dumosa)

  • Joseph Francis Ree,
  • Christy Powell,
  • Raquel Folgado,
  • Valerie C. Pence and ,
  • Christina Walters and
  • Joyce Maschinski
  • + 1 author+ 0 author

20 April 2024

Worldwide, oak species are threatened with extinction due to habitat loss, pathogens, and changing fire regimes. Ex situ conservation through tissue culture may protect the remaining genetic diversity of Quercus dumosa, or the coastal sage scrub oak,...

(This article belongs to the Section Plant Cell Biology)
  • Article
  • Open Access
3 Citations
1,410 Views
10 Pages

6 April 2025

In vitro hedging; combined with the fed-batch liquid media process is an innovative system that generates multiple sterile plants without the use of exogenous cytokinin. This combined process was demonstrated with Cannabis sativa (‘Cherry1&rsqu...

(This article belongs to the Section Plant Development and Morphogenesis)
  • Article
  • Open Access
1 Citations
1,062 Views
18 Pages

Establishment of an Organogenesis-Based Regeneration System and Induction of Somatic Embryogenesis in Catalpa ovata

  • Pingan Bao,
  • Xingping Huo,
  • Jingshuang Sun,
  • Guanzheng Qu and ,
  • Wenjun Ma,
  • Junhui Wang and
  • Ruiyang Hu
  • + 2 authors+ 0 author

10 April 2026

To overcome the seasonal constraints of explant availability and facilitate genetic improvement in Catalpa ovata, this study established a dual-pathway in vitro regeneration system (encompassing adventitious shoot organogenesis and somatic embryogene...

(This article belongs to the Special Issue Sexual and Asexual Reproduction in Forest Plants—2nd Edition)
  • Article
  • Open Access
1,736 Views
14 Pages

Rheological Flow Behavior of Six Gelling Agents and Their Relevance for In Vitro Culture Performance of Medicinal Plants

  • Doina Clapa,
  • Monica Hârţa,
  • Bernadette-Emőke Teleky,
  • Ana-Maria Radomir and ,
  • Adrian George Peticilă and
  • Dorin Ioan Sumedrea
  • + 1 author+ 0 author

12 February 2026

Gelling agents are widely used to solidify plant tissue culture media, yet differences among commercial products may influence the medium’s physical properties and in vitro development of explants. The aim of this study was to characterize the...

(This article belongs to the Special Issue Women’s Special Issue Series: Gels (2nd Edition))
  • Article
  • Open Access
4 Citations
3,653 Views
18 Pages

Optimizing Growth Regulator Concentrations for Cannabis sativa L. Micropropagation

  • Gabrielle A. Johnson,
  • Carissa L. Jackson,
  • Antonio Timoteo,
  • Papaiah Sardaru and ,
  • Michael H. Foland,
  • Purushothaman Natarajan and
  • Sadanand A. Dhekney
  • + 2 authors+ 0 author

20 August 2025

In this study, the effect of growth regulators on shoot proliferation and rooting were evaluated to develop an efficient micropropagation protocol for the Cannabis sativa L. cultivars ‘Cherry Soda’ and ‘Purple’. Apical meriste...

(This article belongs to the Special Issue Plant Tissue Culture and Plant Regeneration—2nd Edition)
  • Article
  • Open Access
7 Citations
3,206 Views
18 Pages

Successful Establishment of Somatic Embryogenesis and Shoot Organogenesis Systems in Catalpa bungei C.A.Mey

  • Jingshuang Sun,
  • Jiewen Li,
  • Mengnan Zhao,
  • Guangshun Zheng and ,
  • Jing Zhang,
  • Bao Di,
  • Wenjun Ma,
  • Junhui Wang and
  • Ruiyang Hu
  • + 4 authors+ 0 author

28 August 2025

Catalpa bungei C.A.Mey is an economically significant deciduous tree valued for timber production and landscaping applications. An efficient regeneration system is crucial for clonal propagation and serves as a foundation for future molecular breedin...

(This article belongs to the Special Issue Sexual and Asexual Reproduction in Forest Plants—2nd Edition)
  • Article
  • Open Access
5 Citations
2,605 Views
16 Pages

Selection for Sustainable Preservation through In Vitro Propagation of Mature Pyrus spinosa Genotypes Rich in Total Phenolics and Antioxidants

  • Styliani Alexandri,
  • Maria Tsaktsira,
  • Stefanos Hatzilazarou,
  • Stefanos Kostas and ,
  • Irini Nianiou-Obeidat,
  • Athanasios Economou,
  • Apostolos Scaltsoyiannes and
  • Parthena Tsoulpha
  • + 3 authors+ 0 author

2 March 2023

Pyrus spinosa Forssk. (almond-leaved pear) is a wild-growing native tree of the hilly countryside of Greece, which has recently gained interest for its valuable endogenous substances. In the present work, the determination of the total phenol content...

(This article belongs to the Special Issue Sustainable Exploitation of Neglected or Underutilized Phytogenetic Resources: From Wild-Growing Native Plants to New Domesticated Crops)
  • Article
  • Open Access
202 Views
38 Pages

Permutation-Centered Henze–Zirkler Screening with Ties: Exact Finite-Sample Centering, Controlled Pairwise Approximation, and Field Assessment

  • Nikita V. Martyushev,
  • Boris V. Malozyomov,
  • Denis V. Valuev,
  • Svetlana N. Sorokova and ,
  • Egor A. Efremenkov,
  • Anton Y. Demin and
  • Alexander V. Pogrebnoy
  • + 2 authors+ 0 author

29 September 2026

PCHZ-SIS (permutation-centered tie-aware Henze–Zirkler sure independence screening) is a specialized marginal screening method for mixed data containing continuous and ordered–discrete predictors with extensive ties. The method combines t...

(This article belongs to the Special Issue Computational Statistics and Applications for High-Dimensional Data Analysis)