Search Results (242)

Contamination of agricultural soils with heavy metals, such as zinc (Zn), copper (Cu) and lead (Pb), is a major problem for food safety and environmental sustainability. The present study aimed to evaluate the efficiency of phytoremediation induced with Brassica juncea (Indian mustard) and ethylenediaminetetraacetic acid (EDTA) in reducing the content of heavy metals in contaminated soils. The experiment was carried out in a greenhouse, using soil polluted with Zn, Cu and Pb, to which different treatments were applied, using: the biological method (Indian mustard only), the chemical method (EDTA in three concentrations: 0.5–1.0–2.0 mmol·kg⁻¹) and the mixed method (Indian mustard and EDTA in three concentrations: 0.5–1.0–2.0 mmol·kg⁻¹). The determinations included the analysis of the residual metal content by atomic absorption spectroscopy, as well as the evaluation of the physiological parameters of the plants (biomass, chlorophyll content in leaves, humidity, height). The results of unifactorial and bifactorial ANOVA revealed highly significant differences (p < 0.001) between the treatments and the types of metals, confirming the synergistic interaction between the chelation and phytoextraction processes. The combined treatments Indian mustard and EDTA in concentrations of 1.0 mmol·kg⁻¹ and 2.0 mmol·kg⁻¹, ensured the highest decontamination efficiency, with reductions of 51.5% for Zn, 36.3% for Pb and 27.5% for Cu. In conclusion, the mixed method represents a viable, ecological and reproducible strategy for the remediation of soils contaminated with heavy metals. Full article

Mustard (Brassica juncea), an essential leaf and oil crop in China, exhibits notable yield potential and adaptability, both of which are influenced by the morphology of the leaf margin. Despite its agronomic importance, the genetic regulatory mechanisms governing this trait remain poorly understood, posing a challenge to molecular breeding efforts. In this study, mustard varieties with lobed and non-lobed leaf margins were used to systematically investigate the genetic basis of leaf margin differentiation through BSA-seq, RNA-seq, and bioinformatics analyses. BSA-seq screening identified four LMI1 homologous genes, including BjuOA10G33260, which may fissure the leaf margin by suppressing cytokinin signaling. RNA-seq analysis revealed significant enrichment of ethylene and growth hormone pathways during key stages of leaf development (at 12 days post-sowing). Integrated analysis of BSA-seq and RNA-seq data identified 15 genes involved in leaf morphogenesis, including BjuOB05G34700 (ADF4, an actin depolymerization factor), BjuOA08G35830 (GATA transcription factor 11), BjuOA09G42060 (ERF transcription factor), and BjuOA07G29650 (GATA transcription factor). Notably, BjuOA10G30380 (TGA2) and BjuOA10G34680 (LAX1) may regulate cytoskeletal dynamics and hormonal signaling, contributing to the development of leaf morphology. This study presents the first molecular network regulating the morphogenesis of the leaf edge in mustard, offering a theoretical foundation and valuable genetic resources for breeding new varieties with optimized leaf architecture. Full article

Microgreens are usually grown on non-edible substrates, whereas edible substrates enable a fully edible, ready-to-eat product. This study evaluated mustard (white, red and green) microgreens grown on an edible gellan gum substrate within a “farm on the fork” system. Agronomical parameters (dry weight, germination, cotyledon area) and bioactive properties (phenolics, antioxidant capacity, phytomelatonin) were assessed during germination. Essential oil supplementation was also evaluated for sensory and antifungal purposes. Gellan gum at 20 g/L was optimal, supporting high germination (red: 95.7%, green: 98.3%, white: 100% at 72 h) and growth without irrigation. After 9 days, white mustard showed the highest fresh weight (63.1 mg/seedling), hypocotyl length (3.52 cm) and cotyledon area (43.5 mm²), while red mustard had the greatest nutraceutical value, with the highest carotenoids (76.4 µg/g FW), flavonoids (4.56 mg/g FW), antioxidant capacity (9.02 µmol TE/g FW) and phytomelatonin (25.5 ng/g FW). Essential oils did not affect biometric traits or antioxidant profile at harvest, although transient rises in flavonoids (+0.34 mg/g FW), antioxidant capacity (+0.97 µmol TE/g FW) and phytomelatonin (two-fold) occurred at early stages (day 3–6). Overall, gellan gum—alone or with essential oils—enabled safe, effective production of ready-to-eat mustard microgreens without compromising growth or nutritional quality. Full article

Between 2023 and 2025, we conducted experiments at the Laboratory Field of the Bio-technical Faculty in Ljubljana to study alternative methods for controlling wireworms in potato fields. The trials were arranged in three blocks with five first-order (Brassica carinata, Brassica juncea, Nemakil 330, Rasti Soil Tonic G, positive control) and five second-order treatments (entomopathogenic nematodes, entomopathogenic fungi, zeolite combined with half-doses of these products, positive control with tefluthrin, and negative control), giving twenty-five treatments per block. Foliar pests and diseases were managed with contact plant protection products. We measured total tuber yield and divided it into three size classes, then assessed wireworm damage (holes per tuber). The purpose of the soil excavations in the first-order treatments was to verify the abundance of wireworms in the soil. Most combinations reduced wireworm abundance. The lowest tuber damage comparable to the positive control occurred when using zeolite with half-doses of entomopathogenic nematodes and fungi. The highest yields across all three weather-distinct years resulted from combining Rasti Soil Tonic with zeolite and half-dose entomopathogenic products. Although Nemakil 330 increased soil phosphorus, it neither improved yield nor reduced wireworm damage. Overall, the tested environmentally acceptable methods show promising insecticidal potential for sustainable wireworm control in potatoes. Full article

This study evaluated the dissipation kinetics and dietary risk of etofenprox in kale (Brassica oleracea) and red mustard greens (Brassica juncea), leafy vegetables frequently reported to exceed residue limits in Korea. Field trials were conducted at three sites, and residues were analyzed using QuEChERS extraction followed by LC–MS/MS in accordance with MFDS and SANTE guidelines. The method validation parameters—specificity, linearity, limit of quantitation, accuracy, and precision—were within the acceptable criteria specified by the guidelines. The half-lives of etofenprox under greenhouse conditions were 2.2 days in kale and 3.1 days in red mustard greens, with dissipation rate constants of 0.3118 and 0.2232, respectively. Dietary risk assessment based on residue levels and consumption data confirmed that the %ADI values at the pre-harvest interval (PHI, 7 days) for were <1% the average consumer group and <4% for the high-intake group. Accordingly, the residue levels were considered safe, indicating that compliance with recommended application practices poses negligible health risk to consumers. Full article

Brassica juncea (L.) Coss. var. foliosa Bailey contains high glucosinolate (GSL) levels that define its flavor and defense properties. However, the regulatory mechanisms controlling GSL biosynthesis in Brassica crops remain unclear. Here, four MYB29 homologs were identified in allotetraploid Brassica juncea. These BjuMYB29 proteins localize to the nucleus and possess transcriptional activation activity. Evolutionary analysis suggests polyploidization-driven expansion of MYB genes contributed to GLS diversification in Brassica species. Expression profiling showed distinct spatiotemporal and herbivory-responsive patterns among BjuMYB29 homologs. Heterologous expression of BjuA03.MYB29 and BjuA10.MYB29 in Arabidopsis enhanced insect resistance via GSL accumulation. Although both homologs upregulate aliphatic GSL biosynthetic genes, they differentially regulate indolic GSLs, with BjuA03.MYB29 suppressing and BjuA10.MYB29 enhancing their accumulation, potentially through differential control of CYP79B2. These results reveal subfunctionalization among MYB29 homologs in GSL regulation. This functional diversification of MYB29 homologs offers novel targets for precision breeding of Brassica crops with customized GSL profiles to optimize pest resistance and nutritional quality. Full article

Salt stress profoundly affects plant growth and metabolism, whereas melatonin has emerged as an effective regulator that modulates plant responses to abiotic stress. In this study, we investigated the interactive effects between salinity (80 and 160 mM NaCl) and exogenous melatonin (100 μM) on the growth, metabolism, and antioxidant capacity of mustard (Brassica juncea) sprouts. The results revealed a synergistic interaction in which melatonin effectively mitigated the inhibitory effects of salinity and optimized the balance between growth and defense metabolism. Under moderate salinity, the combined treatment (MN1) significantly enhanced biomass accumulation, soluble sugars, proteins, and glucosinolate retention, while markedly increasing ascorbic acid, total phenolics, and antioxidant capacity. Principal component and membership function analyses confirmed that the melatonin × salinity interaction improved overall physiological performance more effectively than either factor alone. These results demonstrate that melatonin effectively enhances stress resilience and nutritional quality in mustard sprouts, providing a promising strategy for improving the functional value of sprouting vegetables under salinity conditions. Full article

Brassica juncea is an important leafy vegetable, and flowering time is a key determinant of its yield and quality. In this study, one significantly up-regulated gene, BjuAGL9-2, was identified from RNA-Seq data. qRT-PCR analysis confirmed that BjuAGL9-2 expression was significantly elevated in reproductive organs and reproductive stages. Further five BjuAGL9-2 over-expression (OE) lines were subsequently generated, which showed an early-flowering and pale-yellow leaf phenotype compared to the wild type. qRT-PCR assays found that the mRNA of core floral integrator genes was changed in Arabidopsis OE lines. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays indicated that BjuAGL9-2 interacted with BjuTUA5, BjuZFP7, BjuGSTU5, and BjuMAPK16 in vivo. Sub-cellular localization assays showed that BjuAGL9-2 localizes in the nucleus, whereas its interacting partners localize in the cytoplasm. qRT-PCR assays further revealed that BjuTUA5 and BjuGSTU5 were up-regulated in flower buds, while BjuZFP7 and BjuMAPK16 were down-regulated. During vegetative stages, all four genes were up-regulated in B. juncea. As for BjuAGL9-2 interaction protein-encoding homolog genes, except AtGSTU5, the other three genes were up-regulated in Arabidopsis OE lines. Additionally, qRT-PCR analysis of chlorophyll biosynthesis-related genes showed that 19 of 27 genes were up-regulated, while 8 genes were down-regulated, in Arabidopsis OE lines. Collectively, these findings suggest that BjuAGL9-2 promotes flowering and contributes to the pale-yellow phenotype by regulating its interacting protein-coding genes, floral integrators, and chlorophyll biosynthesis genes. Full article

Antimony (Sb), a toxic metalloid, inhibits plant growth and threatens human health. Yellow Stripe-Like (YSL) proteins play crucial roles in metal ion transport and cellular homeostasis. While the OPT gene family has been characterized in some species, its genome-wide organization and functional involvement in Sb stress response remain unexplored in Brassica juncea. Here, we identified 47 high-confidence BjOPT genes and combined transcriptomic approaches to elucidate their regulatory roles under Sb stress. Phylogenetic tree, conserved motifs, and gene structure analyses consistently distinguished the BjOPT and BjYSL subfamilies. Comparative and collinearity analyses indicated that OPT genes in Brassica species (including B. rapa, B. nigra, and B. juncea) expanded independently of whole-genome triplication events. Transcriptomic profiling revealed significant enrichment of differentially expressed genes (DEGs) related to key biological processes (oxidative and toxic stress response, metal ion transport, and auxin efflux) and pathways (glutathione metabolism, MAPK signaling, and phytohormone transduction), highlighting their roles in Sb detoxification and tolerance. Notably, three BjYSL3 (BjA10.YSL3, BjB02.YSL3, and BjB05.YSL3) genes exhibited strong up-regulation under Sb stress. Heterologous expression in yeast demonstrated that both BjA10.YSL3 and BjB02.YSL3 enhance Sb tolerance, suggesting their potential role in transporting Sb–nicotianamine (NA) or phytosiderophore (PS) complexes. These findings advance our understanding of Sb tolerance mechanisms and provide a basis for developing metal-resistant crops and phytoremediation strategies. Full article

Brassica juncea var. multiceps (Xuelihong), a variety of B. juncea (L.) Czern., holds considerable nutritional and economic value. However, its complete chloroplast genome and the evolutionary relationships within Brassicaceae remain poorly characterized. Using Illumina NovaSeq 6000 high-throughput sequencing, we assembled and annotated the full chloroplast genome sequence of B. juncea var. multiceps. The genome is 153,483 bp in length, with 36.36% GC content, and encodes 132 genes. Codon usage analysis identified leucine (Leu) as the dominant amino acid. Thirty-one codons had relative synonymous codon usage (RSCU; a metric for codon preference) values greater than one, with 93.55% of these preferred codons ending in A/U. We detected 37 dispersed repeats (14 forward, 18 palindromic, 3 reverse, and 2 complementary) and 315 simple sequence repeats (SSRs), with mononucleotide SSRs dominating (72.70%). Analysis of the Ka/Ks ratio, a measure of selection pressure (where values greater than one indicate positive selection), indicated that ycf1, ycf2, and nadhF genes may have undergone positive selection. The nucleotide diversity analysis revealed five hypervariable hotspot-genomic regions with high mutation rates, which are critical for phylogenetic studies. Phylogenetic analysis of 26 Brassicaceae species revealed that B. juncea var. multiceps is closely related to B. juncea. Notably, this is the first complete chloroplast genome of B. juncea var. multiceps, with unique hypervariable regions not reported in other B. juncea varieties. These findings clarify evolutionary relationships in Brassicaceae, provide molecular markers for the genetic breeding of B. juncea var. multiceps, and enhance our understanding of chloroplast genome adaptation in Brassica. Full article

Leaf color is a key trait influencing photosynthetic efficiency in plants. This study investigates the photosynthetic characteristics of differently colored leaves in Brassica juncea L. using green-leaved (SWJ) and purple-red-leaved (RLJ) varieties, their reciprocal F₁ hybrids, and F₂ populations. The results show that the net photosynthetic rate and chlorophyll content of SWJ were significantly higher than those of RLJ, while F₁ hybrids exhibited intermediate photosynthetic performance. All five measured photosynthetic traits—net photosynthetic rate, stomatal conductance, intercellular CO₂ concentration, transpiration rate, and chlorophyll content—segregated significantly in the F₂ generation and were identified as quantitative traits. Notably, transpiration rate was positively correlated with leaf color, whereas no correlation was found with net photosynthetic rate or intercellular CO₂ concentration. A key finding is the occurrence of purple-leaved plants with high photosynthetic rates and green-leaved plants with low photosynthetic rates in the F₂ generation, indicating the potential to combine high photosynthesis with anthocyanin-rich purple leaves. This study provides new genetic insights and a theoretical basis for breeding high-yield, stress-tolerant Brassica juncea varieties. Full article

Environmental dissemination of antibiotics is a pressing global challenge, driving ecological imbalances and the proliferation of antibiotic resistance genes (ARGs). Conventional treatment technologies often fail to fully eliminate these micropollutants or are cost-prohibitive for widespread use. In this context, phytoremediation—using plants and their associated microbiota to remove, transform, or immobilize contaminants—has emerged as an effective and promising, low-impact, and nature-based approach for mitigating antibiotic pollution in aquatic and terrestrial environments. This review provides a comprehensive synthesis of the physiological, biochemical, and ecological mechanisms by which plants interact with antibiotics, including phytoextraction, phytodegradation, rhizodegradation, and phytostabilization. This review prioritizes phytoremediation goals, with attention to high-performing aquatic (e.g., Lemna minor, Eichhornia crassipes, Phragmites australis) and terrestrial plants (e.g., Brassica juncea, Zea mays) and their ability to remediate major classes of antibiotics. This study highlights the role of rhizosphere microbes and engineered systems in phytoremediation, while noting challenges such as variable efficiency, phytotoxicity risks, limited knowledge of by-products, and environmental concerns with antibiotic degradation. Future perspectives include the integration of genetic engineering, microbiome optimization, and smart monitoring technologies to enhance system performance and scalability. Plant-based solutions thus represent a vital component of next-generation remediation strategies aimed at reducing antibiotic burdens in the environment and curbing the rise in antimicrobial resistance. Full article

Annually, about 2.4 million tons of superabsorbent polymers (SAPs) used in disposable diapers are thrown away, polluting our planet. This study aims to explore the potential for reusing SAPs removed from diapers to enhance soil water retention. To this end, the swelling and water retention properties of SAP gels from three different types of diapers were compared to those of an agricultural gel, Aquasorb. Sand was used as a model for soil. When mixed with sand, diaper gels have a swelling degree of ca. 100 g per gram of dried polymer, and a swelling pressure of 12–26 kPa, which are similar to those of Aquasorb gel. Using a synthesized poly(acrylamide-co-sodium acrylate) gel as an example, the correlation between the swelling pressure and the compression modulus of the swollen gel was demonstrated. Soil-hydrological constants were estimated from water retention curves obtained by equilibrium centrifugation of gel/sand mixtures. It was observed that adding 0.3 vol% of diaper gels to sand leads to a 3–4-fold increase in water range available to plants, which is close to that provided by agricultural gel Aquasorb. The water-holding properties were shown to be maintained during several swelling/deswelling cycles in the sand medium. The addition of diaper gels to soil had a significant positive impact on mustard (Brassica juncea L.) seed germination and seedling growth, similar to the agricultural gel Aquasorb. This suggests high potential for the reuse of SAPs from diaper waste to improve soil water retention and water accessibility to plants. This would provide both economic and environmental benefits, conserving energy and raw materials to produce new agricultural gels and limiting the amount of waste. Full article

Disease caused by Plasmodiophora brassicae severely disrupts cruciferous crops by altering root physiology and rhizosphere ecology. While pathogen-induced shifts in rhizosphere microbiomes are documented, the mechanisms linking root exudate reprogramming to microbial community remodeling remain poorly understood. Here, we integrated untargeted metabolomics and 16S rRNA sequencing to investigate how root exudates reshape the rhizosphere microbiome of tumorous stem mustard (Brassica juncea var. tumida) through P. brassicae infection. Metabolomic profiling identified 1718 root exudate metabolites, with flavones (e.g., apigenin 7-O-β-D-rutinoside, VIP > 1.5) and phenolic derivatives (e.g., gastrodin) being selectively enriched in infected plants. P. brassicae infection significantly increased rhizobacterial richness (ACE index, p < 0.05) and restructured the community composition, marked by enrichment of Paenibacillus (LDA score > 3.0). Procrustes analysis revealed tight coupling between microbial community shifts and metabolic reprogramming (M² = 0.446, p = 0.005), while Spearman correlations implicated pathogen-induced metabolites like geniposidic acid in recruiting beneficial Paenibacillus. Our results reveal that plant hosts dynamically secrete defense-related root metabolites to remodel the rhizosphere microbiome in response to P. brassicae infection. This dual-omics approach elucidates a chemical dialogue mediating plant–microbe–pathogen interactions, offering novel insights for engineering disease-suppressive microbiomes through root exudate manipulation. Full article

Heavy metal pollution due to mining activities poses a significant threat to agricultural production, ecosystem health, and food security in South Africa. This review integrates current knowledge on the use of mustard spinach (Brassica juncea (L.) Czern.) for the bioremediation of polluted water and soil, focusing on enhancing phytoremediation efficiency through the use of silicon-based biostimulant treatments. Mustard spinach is known for its capacity to accumulate and tolerate high levels of toxic metals, such as Pb, Cd, and Hg, owing to its strong physiological and biochemical defense mechanisms, including metal chelation, antioxidant activity, and osmotic adjustment. However, phytoremediation potential is often constrained by the negative impact of heavy metal stress on plant growth. Recent studies have shown that silicon-based biostimulants can alleviate metal toxicity by reducing metal bioavailability, increasing metal immobilization, and improving the antioxidative capacity and growth of plants. Combining silicon amendments with mustard spinach cultivation is a promising, eco-friendly approach to the remediation of mining-impacted soils and waters, potentially restoring agricultural productivity and reducing health risks to the resident populations. This review elucidates the multifaceted mechanisms by which silicon-enhanced phytoremediation operates, including soil chemistry modification, metal sequestration, antioxidant defense, and physiological resilience, while highlighting the practical, field-applicable benefits of this combined approach. Furthermore, it identifies urgent research priorities, such as field validation and the optimization of silicon application methods. Full article