Search Results (148)

Valsa canker, a destructive necrotrophic disease caused by Valsa species (V. mali and V. pyri), threatens global apple and pear production. Resistance breeding has gained much attention because of its environmental friendliness and effectiveness, making it extremely critical to obtain key disease resistance genes. In this study, we identified that the expression of PbeSTOP2, a C2H2-type transcription factor in ‘Duli-G03’ (Pyrus betulifolia, rootstock of pear), was induced in response to signals from Vp and Vp metabolites (VpM). Transient expression in fruits and stable overexpression in suspension cells demonstrated that PbeSTOP2 enhances resistance to Vp. In overexpressing cells, genes associated with jasmonic acid (JA) and pattern-triggered immunity (PTI) signaling were upregulated, and endogenous JA and auxin (IAA) levels increased. These results revealed that JA signaling was involved in the enhanced Valsa canker resistance conferred by PbeSTOP2 overexpression. This finding on host disease resistance should facilitate the breeding efforts for Valsa canker resistance. Full article

Due to its significant environmental impact, the built environment faces growing pressure to transition toward more sustainable practices. Biomimicry, a novel field of practice that entails design and innovation inspired by nature’s time-tested strategies, offers a promising pathway to enhance sustainability in the construction industry. Hence, this study examines the perceived benefits of applying biomimicry principles in the construction sector, aiming to identify the key dimensions that underpin its transformative potential. An exploratory factor analysis (EFA) was conducted using data collected through a structured questionnaire survey, which contained 18 indicators derived from a targeted literature synthesis. The questionnaire was administered to 120 purposively sampled, duly registered, practising construction and biomimicry professionals in South Africa. The instrument captured perceptions of the environmental, economic, and socio-functional benefits of adopting and implementing biomimicry. The EFA revealed four principal factors: socio-economic and health, ecological resilience, performance enhancement and green market efficiency. These four factors cumulatively accounted for approximately 70% of the total variance, indicating a strong internal structure of perceived benefits. The findings demonstrate that stakeholders perceive biomimicry as a tool for reducing environmental footprints and as a catalyst for innovation, circularity, and regenerative design practices in the built environment. This research contributes to the emerging discourse on biomimicry in the built environment by providing empirical evidence on its multifaceted value. It highlights the importance of integrating natural design intelligence into construction to foster more adaptive, efficient, resilient and sustainable systems. The paper recommends policy support, interdisciplinary collaboration, and further research to operationalise biomimicry within mainstream construction processes. Full article

Although the hub blockage effect is generally disregarded for large-sized horizontal axis wind machines, it can significantly affect the performance of small-sized turbines whose ratio between the hub and rotor radii can attain values up to 25–30%. This article proposes a generalisation of the Blade-Element/Momentum Theory (BE/M-T), accounting for the effects of the hub presence on the rotor performance. The new procedure relies on the quantitative evaluation of the radial distribution of the axial velocity induced by the hub all along the blade span. It is assumed that this velocity is scarcely influenced by the magnitude and type of the rotor load, and it is evaluated using a classical CFD approach applied to the bare hub. The validity and accuracy of the modified BE/M-T model are tested by comparing its results with those of a more advanced CFD-actuator-disk (CFD-AD) approach, which naturally and duly takes into account the hub blockage, the rotor presence, an and the wake divergence and rotation, and the results are validated against experimental data. The comparison shows that the correction for the hub blockage effects in the BE/M-T model significantly reduces the differences with the results of the reference method (CFD-AD) both in terms of global (power coefficient) and local (thrust and torque per unit length) quantities. Full article

We investigate a combined conservative field, in which classical gravitational and electrostatic sources also exhibit mutual interactions. Hitherto neglected, the coupling between mass and charge may be necessary for constructing a unified conservative force field generated by a single underlying source. We determine the coupling constant of the cross-field components as the geometric mean (G-M) of Newton’s G and Coulomb’s K constants, in both SI units and dimensionless form. Consequently, for two identical objects, the cross-force ($F_{\times }$) is the G-M of the familiar Newton ($F_{\mathrm{g}}$) and Coulomb ($F_{\mathrm{e}}$) forces, so that $F_{\times }=\sqrt{F_{\mathrm{g}}{F}_{\mathrm{e}}}$, where $F_{\mathrm{g}}\ll F_{\times }\ll F_{\mathrm{e}}$. Remarkably, such cross-forces should be measurable in torsion balance experiments involving a suspended neutral mass interacting with a partially ionized gas. Furthermore, we apply our new formulation to estimate the dimensionless amplitude $\parallel {\mathcal{h}}_{\alpha \beta }^{\mathrm{TT}}\parallel$ of gravitational waves that are emitted by inspiraling Reissner–Nordström (RN) black hole binaries, expressed in terms of ratios of the four fundamental lengths of the problem: the distance to the binary D, the binary separation R, the Schwarzschild radius $R_S\propto 2M$ of mass M, and the RN charge (Q) length scale $L_Q\propto 2Q$. In this classical setting with speeds much lower than the speed of light c in vacuum, the surprising appearance of the maximum relativistic tension force $F_{\max }=c^4/\left(4G\right)$ is duly noted. Full article

The Ōbaku Edition of the Buddhist Canon, initiated and spearheaded by Tetsugen Dōkō (鐵眼道光 1630–1682), profoundly influenced Japanese Buddhism and printing culture. Although the Ōbaku Edition has long been recognized as a product of cross-border collaboration, earlier studies have primarily focused on its textual features and religious significance after publication. As a result, the specific mechanisms and significance of its transnational nature have remained underexplored. This study revisits the canon’s compilation as a complex trans-sectarian and transnational social practice. Drawing on a variety of sources, it provides new insights into the production of the Ōbaku Canon, showing that this trans-sectarian project was driven by the interplay of several key dynamics: transnational networks supplying its base text, intellectual contributions from Chinese migrants, local social needs in post-fire Nagasaki, and Tetsugen’s personal aim to use the canon in doctrinal debate. Tetsugen’s printing endeavor continued the medieval tradition of kanjin (fundraising), serving as a religious, educational, and institutional undertaking shaped by interactions with broader socio-economic support. Positioned within a wider social framework, Tetsugen’s cross-sectarian activities facilitated the successful circulation and popularization of the canon, promoting a shift in the use of Buddhist scriptures from prayer-centered practice to doctrinal study and reflecting a broader transformation in Japanese Buddhism, from state-supported Buddhism to more popular forms. Full article

We investigated the effect of Agrobacterium rhizogenes-mediated transformation mof rolB on adventitious root development and endogenous hormones in ‘duli’ (Pyrus betulaefolia) via transcriptomic analysis of wild-type (WT) and rolB-transformed plants. The formation of root primordia occurred earlier in transgenic ‘duli’ than in the WT plants. At seven days, 57% of the transgenic seedlings had formed root primordia, whereas root primordia first appeared at seven days in WT ‘duli’. The rooting rate of transgenic ‘duli’ and WT plants was 90% and 77.14%, respectively. rolB significantly promoted the formation of secondary roots. Within 20 days, auxin (IAA), gibberellic acid (GA₃), and zeatin riboside (ZR) were higher and abscisic acid (ABA) was lower in transgenic ‘duli’ than in WT plants. Gene Ontology analysis revealed high enrichment in signaling pathways and ADP binding, and Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that several differentially expressed genes were enriched in flavonoid and carotenoid-related pathways and plant hormone signal transduction. rolB induced changes in the expression patterns of several genes involved in hormone biosynthesis, metabolism, and signal transduction pathways in ‘duli’. Weighted gene co-expression network analysis identified the DEGs associated with endogenous hormone levels and indicated that the central genes of modules most strongly correlated with ABA, ZR, IAA, and GA₃ regulate protein synthesis, signaling, and root tissue meristem activity. Protein–protein interaction analysis yielded a co-expression network of physiological and transcriptomic data during rooting and identified key genes at the network core. These findings provide valuable insights into the regulatory mechanisms of rolB and its influence on root development in ‘duli’. Full article

The fw2.2 (fruit weight 2.2) gene negatively regulates cell division and significantly influences fruit size, but its regulatory mechanisms in pears remain unclear. Here, we investigated how pear FWL (fw2.2-like) genes control cell division using Duli pear, Korla fragrant pear, and Yali pear. During the cell division phase, fluorescence in situ hybridization (FISH) revealed stronger expression of FWL1 and FWL5 in smaller fruits compared to larger ones, with both genes localized in the core and flesh tissues. Gene silencing experiments demonstrated that silencing of FWL5 leads to a significant increase in the number of cells, with a concomitant enlargement of the fruit. Yeast two-hybrid screening identified 147 proteins interacting with FWL5, showing substantial overlap with FWL1 interactors. Key candidates included metallothionein-like protein (MT) and Ole-e-1, with the latter displaying a positive correlation with fruit size during cell division. Bimolecular fluorescence complementation (BiFC) confirmed direct interactions between Ole-e-1 and both FWL1/FWL5. Functional analysis indicated the Ole-e-1 gene family has diverse roles in pear development. We propose that Ole-e-1 interacts with FWL genes to modulate cell division, thereby determining final fruit size. This study uncovers a novel regulatory axis linking cell cycle control and fruit size in pears. Full article

This study aimed to determine herd prevalence of tail alterations (deviated and shortened tails) on farms in Germany. In total, 86,355 cows on 765 dairy farms in three regions (North, South, East) were assessed for presence or absence of deviated and shortened tails. Mean herd prevalence of deviated tails was 10.00% with a median of 7.89%. The mean herd prevalence of shortened tails was 1.07% with a median of 0%. Herd size, energy-corrected milk yield (ECM), and husbandry system were associated with herd prevalence rates of tail deviations and shortened tails. Herd prevalence increased with herd size (Spearman’s ρ = 0.586) and median ECM (ρ = 0.404). 28.10% farms had a herd prevalence <5% tail alterations, while 26.41% had a herd prevalence of >15%. Our findings plead for including monitoring of tail alterations in animal welfare assessments of dairy farms. The National Dairy FARM Program in New Zealand aims to achieve a herd prevalence of less than 5%. A herd prevalence of >15% tail alterations is regarded as the critical threshold requiring clarification and elimination of causes. It is imperative that all dairy farms strive to achieve a herd prevalence of less than 5% and welfare protocols are duly adjusted. Full article

Fire blight, caused by Erwinia amylovora, is a devastating bacterial disease threatening apple, pear, and other Rosaceae species. In our prior study, transcriptome analysis identified a fire blight-resistant variety, Duli (Pyrus betulifolia Bunge), and highlighted the PR1 gene as a key resistance factor. Using Duli’s genomic data, we systematically identified and characterized the Pb-PR-1 gene family through bioinformatics analysis. A total of 31 Pb-PR-1 genes were found, encoding proteins of 123–341 amino acids. Phylogenetic analysis grouped these genes into four subfamilies, with 27 genes distributed across seven chromosomes, all contain a conserved CAP superfamily domain. Their promoter regions were enriched in hormone and stress-responsive elements. After inoculation with E. amylovora, susceptible Duli showed lesion development by day 2, with rapid disease progression, while resistant plants exhibited slower disease advancement and smaller lesions. Enzyme activity assays revealed that in resistant plants, PPO (polyphenol oxidase) and CAT (catalase) activities peaked on day 6, showing a 2.4-fold and 3.81-fold increase compared to susceptible Duli. At the same time, MDA (malondialdehyde) content decreased by 16.6%. The activities of SOD (superoxide dismutase) and PAL (phenylalanine ammonia-lyase) peaked on day 4, with increments of 34.32% and 47.1% over susceptible Duli. qRT-PCR showed significant differences in Pb-PR-1 gene family expression between resistant and susceptible plants post-inoculation. Notably, Pb-PR-1-11, Pb-PR-1-21, and Pb-PR-1-26 expression increased with infection duration, aligning with PPO and CAT activity trends. Other genes showed high early infection expression but declined by day 6. Pb-PR-1-3, Pb-PR-1-6, Pb-PR-1-8, Pb-PR-1-16, and Pb-PR-1-30 were upregulated 13.17-fold on average by day 2. In summary, the Pb-PR-1 family exhibited elevated expression during early infection and enhanced defense-related enzyme activities, improving plant resistance. This study provides a foundation for understanding the PR-1 family’s role in Duli and advancing fire blight resistance in Pyrus species. Full article

In order to ensure the continuous and reliable supply of electrical energy to the power grid, it is necessary to evaluate and monitor the degree of impairment of the insulation of electrical cables, as throughout its service life, insulation around cables suffers degradation due to numerous stress factors, which can arise from both environmental and operational causes. This aspect has aroused deep interest among energy professionals, as well as the industrial sector, with focus mainly placed on the undesirable effect caused by unexpected and sudden process stoppages, as well as their consequent financial and social impacts. That said, this article presents a methodology for evaluating the degree of insulation aging using the partial discharge progression curve. For this purpose, a thermal oven was duly constructed, in accordance with the technical premises presented in the literature, capable of homogeneously heating conductor samples. After thermal cycles, these conductors were aptly handled and tested in a controlled laboratory environment to determine the partial discharge progression curve. Through accurate data processing, a correlation was obtained between the degradation of the insulation and the rate of increase in partial discharge. The results are promising, as they provide support for maintenance agents’ ability to monitor and intervene regarding conductors. Full article

To duly and correctly deliver parcels, both the capacity and the delivery route of a delivery vehicle need to be considered. Thus, the delivery process of a delivery vehicle can be characterized as a capacitated vehicle routing problem with three-dimensional loading constraints (3L-CVRP), which is an NP-hard problem. To solve the problem, a mathematical model is established in this paper to minimize the total delivery distance and maximize the loading rate, simultaneously. Additionally, a hybrid algorithm that combines a three-dimensional (3D) packing algorithm based on the residual space optimized (RSO) strategy and an improved genetic algorithm (IGA) is proposed. Initially, the proposed hybrid algorithm employs a modified Clarke–Wright savings algorithm to generate a feasible set of route solutions. Furthermore, building upon the traditional genetic algorithm, an elite retention strategy is introduced, and an enhanced order crossover method is utilized to improve the stability of the hybrid algorithm and its global search capability for optimal solutions. Finally, during each iteration of the algorithm, the RSO algorithm is integrated to verify the feasibility of 3D packing scheme. Two comparative experiments are conducted on 22 modified benchmark instances and actual logistics data of a university against two other algorithms, demonstrating that the proposed RSO-IGA algorithm achieves superior solutions in delivery efficiency. Full article

Porphyrinoids are suitable sensitive materials for potentiometric electronic tongues. In this paper, we take advantage of these properties to develop an electronic tongue using an extended-gate field-effect transistor as a signal transducer. The sensitive films were made of different porphyrins and corroles electropolymerized in situ onto laser-induced graphene electrodes. The electronic tongue was duly characterized with respect to ascorbic acid, a common natural antioxidant. The sensors were shown to be sensitive and selective with respect to common interferents, such as dopamine and uric acid. Finally, the sensors were tested to detect ascorbic acid in artificial tears. Full article

The phosphate transporter (PHT) family plays an important role in the uptake and transport of P elements in plants. A total of 158 PbPHTs were identified from the genome of ‘duli’ (Pyrus betulifolia Bunge) in this study, including 70 PbPHT1s, 2 PbPHT2s, 70 PbPHT3s, 12 PbPHT4s, and 4 PbPHT5s. Among the 158 PHT genes, 150 were localized to 17 ‘duli’ chromosomes. Gene duplication analysis identified 18 tandemly duplicated gene pairs. The promoter analysis showed that there were a large number of cis-acting elements related to phytohormones, growth, development, stress, and light response in PbPHTs. qRT-PCR analysis revealed that most PHT genes in ‘duli’ were highly expressed in the fruits, flowers, leaves, stems, and roots, and 15 PbPHT genes were responsive to 5 μM, 0.5 mM, 5 mM H₂PO₄, NaCl, GR24 (synthetic SL analog), GA3 (gibberellin 3), ABA (abscisic acid), and IAA (indole-3-acetic acid). GR24, GA3, IAA, and 5 mM KH₂PO₄ treatments could increase the concentration, absorption, transport, and distribution of P elements in the rhizomes and leaves of ‘duli’, but 5 μM KH₂PO₄, NaCl, and ABA had the opposite effect. This study therefore provides a list of PbPHT genes with substantial roles in abiotic stress response, as well as important information to understand the functional characteristics of PbPHT during ‘duli’ abiotic stress tolerance, and explores the function of PbPHTs in exogenous hormones, phosphorus, and salt stress in the future. Full article

Cytokinin oxidases/dehydrogenases (CKXs) play a crucial role in modulating plant stress resistance by degrading cytokinins. The ‘duli’ pear (Pyrus betulifolia Bunge), a highly stress-resistant cultivar, is widely used as a rootstock in pear cultivation. This study aims to comprehensively identify and characterize the PbCKX gene family in ‘duli’. A total of 10 PbCKX genes were identified, which are unevenly distributed across five chromosomes and classified into four groups based on sequence similarity and phylogenetic relationships. The PbCKX genes exhibit a high degree of conservation in motifs and structural features, although exon structure variations are observed. Comparative analysis revealed 10 homologous gene pairs between ‘duli’ and Arabidopsis and 14 pairs between ‘duli’ and apple. Additionally, cis-acting elements related to abiotic stress, hormone responses, and light responsiveness were identified in the promoter regions of the PbCKX genes. RNA-seq analysis showed that PbCKX1 and PbCKX2 were predominantly expressed in roots, while PbCKX3 to PbCKX10 had higher expression in leaves. The PbCKX genes responded to both exogenous hormones and salt stress, with salt stress inducing a more pronounced response. Most abiotic stress treatments led to the downregulation of PbCKX4 and PbCKX9, while PbCKX6 and PbCKX8 were upregulated. Notably, treatments with Abscisic acid and NaCl significantly enhanced CKX enzyme activity in ‘duli’ over 20 days, reducing levels of zeatin and isopentenyladenine. Conversely, treatments with gibberellin, cytokinin, and auxin significantly reduced CKX enzyme activity and increased concentrations of zeatin and isopentenyladenine over the same period. These findings provide valuable insights for future studies on the functional role of PbCKX genes in abiotic stress responses in ‘duli’. Full article

SUPPRESSOR OF MAX2 1-LIKE (SMXL) proteins are negative regulators of strigolactone (SL) signal transduction that play an important role in regulating plant branching and responses to abiotic stress. Here, we studied the role of SMXL proteins in pear growth, development, and stress resistance. A total of 18 SMXL members were characterized in ‘duli’. All SMXL members were localized to chloroplasts. Chromosome mapping analysis showed that the members of this family were unevenly distributed on 14 chromosomes. Gene fragment replication analysis showed that there were no tandem repeat genes in PbSMXLs, and 12 pairs of homologous genes were fragment duplications. There were 30 pairs of homologous genes between ‘duli’ and apples, and 17 between ‘duli’ and Arabidopsis thaliana. Analysis of cis-acting elements showed that there was a large number of photo-effector elements, short-effector elements, hormone-responsive elements, and abiotic stress-responsive elements in the promoter sequences of this family. Analysis of enzyme activity and endogenous SL showed that β-carotenoid isomerase (D27), carotenoid cleavage dioxygenase 7 (CCD7), lateral branch oxidoreductase (LBO) levels, and SL content were higher in ‘duli’ roots and leaves compared in the control under exogenous GA₃ (gibberellin 3), IAA (indole-3-acetic acid), GR24 (synthetic SL analog), and NaCl. Most SMXL genes in ‘duli’ were highly expressed in branches and axillary lobes, but their expression was low in fruits. qRT-PCR analysis revealed that eight PbSMXL genes were responsive to GA₃, PAC (Paclobutrazol), IAA, ABA (abscisic acid), GR24, and Tis108 (SL biosynthesis inhibitor). PbSMXLs responded positively to salt stress. The expression of PbSMXL6 and PbSMXL15 was significantly induced under salt stress. The expression of PbSMXL7, PbSMXL10, and PbSMXL15 was significantly induced by Tis108 treatment. The results of this study enhance our understanding of the role of SMXL genes in the responses to plant growth regulators and salt stress. Our findings will also aid future studies of the functions of SMXL genes in ‘duli’. Full article